鞘氨醇单胞杆菌Sphigomonas sp.WG产威兰胶的提取工艺研究
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摘要
选用乙醇作为沉淀剂,对鞘氨醇单胞杆菌Sphigomonas sp.WG产威兰胶的提取工艺进行设计和优化。考察了发酵液加热处理、固液分离方式、乙醇用量、沉淀温度和沉淀时间对威兰胶提取量的影响,同时研究了3种不同干燥方式对威兰胶溶液粘度的影响。优化后的威兰胶醇沉工艺为:发酵液与乙醇的混合比例为1∶5.5(体积比),乙醇沉淀温度和时间分别为20℃和6 h,威兰胶沉淀物采用过滤分离,最后经真空冷冻干燥获得成品。
The extraction process of Welan gum from Sphigomonas sp. WG was designed and optimized with ethanol as a precipitator. The influences of fermentation liquor heating treatment, solid-liquid separation method, ethanol dosage, precipitation temperature and time on Welan gum yields were investigated. In addition, the effects of three drying methods on the viscosity of Welan gum were also examined. The optimized extraction process was set as follows. The mixing ratio between fermentation liquor and ethanol was 1∶5.5(volume ratio), the precipitation temperature and time were 20 ℃ and 6 h respectively. Welan gum precipitation was separated by filtration, and the finished product of Welan gum was obtained by vacuum freeze-drying the samples.
The extraction process of Welan gum from Sphigomonas sp. WG was designed and optimized with ethanol as a precipitator. The influences of fermentation liquor heating treatment, solid-liquid separation method, ethanol dosage, precipitation temperature and time on Welan gum yields were investigated. In addition, the effects of three drying methods on the viscosity of Welan gum were also examined. The optimized extraction process was set as follows. The mixing ratio between fermentation liquor and ethanol was 1∶5.5(volume ratio), the precipitation temperature and time were 20 ℃ and 6 h respectively. Welan gum precipitation was separated by filtration, and the finished product of Welan gum was obtained by vacuum freeze-drying the samples.
引文
[1]LI H,XU H,XU H,et al.Biosynthetic pathway of sugar nucleotides essential for Welan gum production in Alcaligenes sp.CGMCC2428[J].Applied Microbiology and Biotechnology,2010,86(1):295-303.
[2]TAKO M,KIRAKI M.Rheological properties of Welan gum in aqueous media[J].Agricultural & Biological Chemistry,1990,54(12):771-776.
[3]ASUBIARO A,SHAH S N.Rheological and hydraulic properties of Welan gum fluids in straight and coiled tubings[J].Journal of Fluids Engineering Transactions of the ASME,2008,130(8):920-926.
[4]许晓鹏,郑志永,朱莉,等.Sphingomonas sp.生产威兰胶分批发酵动力学[J].食品与生物技术学报,2015,34(5):507-511.XU Xiaopeng,ZHENG Zhiyong,ZHU Li,et al.Study on Welan gum fermentation dynamics of Sphingomonas sp.[J].Journal of Food Science and Biotechnology,2015,34(5):507-511.
[5]CAMPANA S,ANDRADE C,MILAS M,et al.Polyelectrolyte and rheological studies on the polysaccharide Welan[J].International Journal of Biological Macromolecules,1991,12(6):379-384.
[6]FIALHO A M,MOREIRA L M,GRANJA A T,et al.Occurrence,production,and applications of gellan:Current state and perspectives[J].Applied Microbiology and Biotechnology,2008,79(6):889-900.
[7]曹花.微生物多糖威兰胶的提取工艺研究[D].无锡:江南大学,2010:4-6.
[8]JASSON PE,WIDMALM G.Welan gum(S-130) contains repeating units with randomly distributed L-mannose and L-rhamnosyl terminal groups as determined by FABMS[J].Carbohydrate Research,1994,256(2):327-330.
[9]MWN H,MORRIS E R.Solubility,solution rheology and salt-induced gelation of Welan polysaccharide in organic solvents [J].Carbohydrate Polymers,1995,27(1):23-36.
[10]LI H,JIAO X,SUN Y W,et al.The preparation and characterization of a novel sphingan WL from marine Sphingomonas sp.WG[J/OL].Scientic Reports,2016,6:37899.
[11]LI H,FENG Z M,SUN Y J,et al.Draft genome sequence of Sphingomonas sp.WG,a Weilan gum-producing strain[J/OL].Genome Announcements,2016,4(1):e01709-15.
[12]LOPES L,MILAS M,RINAUDO M.Influence of the method of purification on some solution properties of Weilan gum[J].International Journal of Biological Macromolecules,1994,16(5):253-258.
[13]刘莎.微生物多糖的制备和应用研究[D].大连:大连工业大学,2010:3-5.
[14]周浩然.微生物多糖威兰胶生产工艺的研究[D].无锡:江南大学,2009:9-15.
[15]陈芳.威兰胶生产工艺初探及流变特性研究[D].南昌:南昌大学,2007:15-27.
[2]TAKO M,KIRAKI M.Rheological properties of Welan gum in aqueous media[J].Agricultural & Biological Chemistry,1990,54(12):771-776.
[3]ASUBIARO A,SHAH S N.Rheological and hydraulic properties of Welan gum fluids in straight and coiled tubings[J].Journal of Fluids Engineering Transactions of the ASME,2008,130(8):920-926.
[4]许晓鹏,郑志永,朱莉,等.Sphingomonas sp.生产威兰胶分批发酵动力学[J].食品与生物技术学报,2015,34(5):507-511.XU Xiaopeng,ZHENG Zhiyong,ZHU Li,et al.Study on Welan gum fermentation dynamics of Sphingomonas sp.[J].Journal of Food Science and Biotechnology,2015,34(5):507-511.
[5]CAMPANA S,ANDRADE C,MILAS M,et al.Polyelectrolyte and rheological studies on the polysaccharide Welan[J].International Journal of Biological Macromolecules,1991,12(6):379-384.
[6]FIALHO A M,MOREIRA L M,GRANJA A T,et al.Occurrence,production,and applications of gellan:Current state and perspectives[J].Applied Microbiology and Biotechnology,2008,79(6):889-900.
[7]曹花.微生物多糖威兰胶的提取工艺研究[D].无锡:江南大学,2010:4-6.
[8]JASSON PE,WIDMALM G.Welan gum(S-130) contains repeating units with randomly distributed L-mannose and L-rhamnosyl terminal groups as determined by FABMS[J].Carbohydrate Research,1994,256(2):327-330.
[9]MWN H,MORRIS E R.Solubility,solution rheology and salt-induced gelation of Welan polysaccharide in organic solvents [J].Carbohydrate Polymers,1995,27(1):23-36.
[10]LI H,JIAO X,SUN Y W,et al.The preparation and characterization of a novel sphingan WL from marine Sphingomonas sp.WG[J/OL].Scientic Reports,2016,6:37899.
[11]LI H,FENG Z M,SUN Y J,et al.Draft genome sequence of Sphingomonas sp.WG,a Weilan gum-producing strain[J/OL].Genome Announcements,2016,4(1):e01709-15.
[12]LOPES L,MILAS M,RINAUDO M.Influence of the method of purification on some solution properties of Weilan gum[J].International Journal of Biological Macromolecules,1994,16(5):253-258.
[13]刘莎.微生物多糖的制备和应用研究[D].大连:大连工业大学,2010:3-5.
[14]周浩然.微生物多糖威兰胶生产工艺的研究[D].无锡:江南大学,2009:9-15.
[15]陈芳.威兰胶生产工艺初探及流变特性研究[D].南昌:南昌大学,2007:15-27.
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