不同暴露方式下TBBPA对日本虎斑猛水蚤的毒性影响的比较研究
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摘要
四溴双酚A (TBBPA)是一种持久性有机污染物(Persistent Organic Pollutants),广泛存在于空气、水体、土壤以及沉积物中。本研究选取海洋桡足类日本虎斑猛水蚤(Tigriopus japonicus)为模式生物,以1/10 96h-LC_(50)为实验浓度,研究了饵料暴露和水体暴露两种暴露方式下TBBPA对日本虎斑猛水蚤的毒性影响。结果显示,两种暴露方式下日本虎斑猛水蚤无节幼虫变态率均在90%以上(P>0.05)。水体暴露组日本虎斑猛水蚤的发育和生殖没有受到显著影响(P>0.05),而饵料暴露组日本虎斑猛水蚤无节幼虫发育至成体的时间、第一只挂卵雌体出现的时间以及产卵量均受到显著影响(P<0.05)。这表明TBBPA能对日本虎斑猛水蚤的发育繁殖产生影响,暴露方式会影响其毒性效应,日本虎斑猛水蚤更易受到饵料中TBBPA的影响。
Tetrabromobisphenol A(TBBPA) is the most widely used brominated flame retardant in the world.It is a potential persistent organic pollutant and has toxic effects on organisms.In the paper,Tigriopus japonicus was selected as a model organism to study the effects of TBBPA on the reproduction and development of the T. japonicus in different exposure methods. The 96 h-LC_(50) of TBBPA effect on T. japonicus was 3.106 mg/L.The experiment was divided into three groups: the control group, the algae exposure group and the water exposure group. The study found that the water exposure group had no significant effect on the development and reproduction of the T. japonicus(P>0.05), However, the algae exposure group significantly shortened the development time of Tigriopus japonicus, the time of emergence of the first ovipositor and the significant increase of the fecundity of T. japonicus(P<0.05). The accumulation of TBBPA in T. japonicus be affected by exposure methods,and the main way T. japonicus accumulates TBBPA is algae exposure method.
Tetrabromobisphenol A(TBBPA) is the most widely used brominated flame retardant in the world.It is a potential persistent organic pollutant and has toxic effects on organisms.In the paper,Tigriopus japonicus was selected as a model organism to study the effects of TBBPA on the reproduction and development of the T. japonicus in different exposure methods. The 96 h-LC_(50) of TBBPA effect on T. japonicus was 3.106 mg/L.The experiment was divided into three groups: the control group, the algae exposure group and the water exposure group. The study found that the water exposure group had no significant effect on the development and reproduction of the T. japonicus(P>0.05), However, the algae exposure group significantly shortened the development time of Tigriopus japonicus, the time of emergence of the first ovipositor and the significant increase of the fecundity of T. japonicus(P<0.05). The accumulation of TBBPA in T. japonicus be affected by exposure methods,and the main way T. japonicus accumulates TBBPA is algae exposure method.
引文
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[24] 林汝榕,李少菁.铜、镉对中华哲水蚤氨基酸含量影响的实验研究[J].海洋与湖沼,1991,22(3):242-248.Lin R R,Li S J.Effects of copper and cadmium on amino acids in Calanus sinicus[J].Chinese Journal of Oceanology and Limnology,1991,22(3):242-248.
[25] 王晓敏,朱丽岩,陈学超,等.六溴环十二烷对拟长腹剑水蚤的急性毒性和生殖发育毒性效应[J].中国海洋大学学报(自然科学版),2017,47(1):82-88.Wang X M,Zhu L Y,Chen X C,et al.Toxic Effect of HBCD on the acute toxicity,development and reproduction of Oithona Similes[J].Periodical of Ocean University of China,2017,47(1):82-88.
[26] 巩文静.四溴双酚A(TBBPA)对河口常见桡足类的毒性效应及其在中国北方海域的污染分布状况[D].青岛:中国海洋大学,2016.Gong W J.Toxic Effects of Tetrabromobisphenol A (TBBPA) on Copepods in the Estuary and Its Pollution Distribution in Northern China[D].Qingdao:Ocean University of China,2016.
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[28] Davies R,Zou E.Polybrominated diphenyl ethers disrupt molting in neonatal Daphnia magna[J].Ecotoxicology,2012,21(5):137-138.
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[2] Jakobsson K,Thuresso K,Rylander L,et al.Exposureto polybrominated diphenyl ethers and tetrabromobis-phenol A among computer technicians [J].Chemosphere,2002,46(5):709- 716.
[3] Morris S,Allchin C R,Zegers B N,et al.Distribution and fate of HBCD and TBBPA brominated flame retardants in North Sea estuaries and aquatic food webs[J].Environmental Science & Technology,2004,38(21):5497-504.
[4] 薛银刚,王晓蓉,顾雪元,等.四溴双酚A对赤子爱胜蚓的急性毒性及抗氧化防御系统酶的影响[J].生态毒理学报,2009,4(1):93-100.Xue Y G,Wang X R,Gu X Y,et al.Acute toxicity of tetrabromobisphenol-A to Earthworms Eisenia fetida and its effects on antioxidant defense system enzymes [J].Asian Journal of Ecotoxicology,2009,4(1):93-100.
[5] 杜苗苗,张娴,颜昌宙.溴系阻燃剂的毒理学研究进展[J].生态毒理学报,2012,7(6):575-584.Du M M,Zhang X,Yan C Y.Advance in researches on toxicology of brominated flame retardants[J].Asian Journal of Ecotoxicology,2012,7(6):575-584.
[6] 郑重,李少菁,许振祖.海洋浮游生物学[M].北京:海洋出版社,1984.Zhang Z,Li S Z,Xu Z Z.Marine Planktology[M].Beijing:China Ocean Press,1984.
[7] Kwok K W H,Leung K M Y.Toxicity of antifouling biocides to the intertidal harpacticoid copepod Tigriopus japonicus,(Crustacea,Copepoda):Effects of temperature and salinity[J].Marine Pollution Bulletin,2005,51(8-12):830-837.
[8] Li L,Guo D H,Zhu X M.Study on acute toxicity of triazophos and acephate on marine copepods[J].Journal of Xiamen University,2008.
[9] Raisuddin S,Kwok K W H,Leung K M Y,et al.The copepod Tigriopus:A promising marine model organism for ecotoxicology andenvironmental genomics[J].Aquatic Toxicology,2007,83(3):161-173.
[10] Andersson P L,Berg A H,Bjerselius R,et al.Bioaccumulation of selected PCBs in zebrafish,three-spined stickleback,and arctic char after three different routes of exposure[J].Archives of Environmental Contamination & Toxicology,2001,40(4):519-30.
[11] Zhou H Y,Cheung R Y H,Wong M H.Bioaccumulation of organochlorines in freshwater fish with different feeding modes cultured in treated wastewater[J].Water Research,1999,33(12):2747-2756.
[12] 刘树苗.四溴双酚 A 对中华哲水蚤(Calanus sinicus)摄食、代谢及抗氧化防御系统的影响[D].青岛:中国海洋大学,2012:17-18.Liu S M.The Effects of Tetrabromobisphenol-A on Ingest-Ion,Metabolism and Antioxidant Defense System of Calanus sinicus[D].Qingdao:Ocean University of China,2012:17-18.
[13] 巩文静,朱丽岩,郝雅,等.四溴双酚 A 对太平洋真宽水蚤的急性毒性及氧化胁迫[J].生态毒理学报,2016(4):232-238.Gong W J,Zhu L Y,Hao Y,et al.Acute toxicity and oxidative stress of tetrabromobis-phenol A to Eurytemora pacifica [J].Asian Journal of Ecotoxicology,2016(4):232-238
[14] Kwok K W H.,Leung K M Y.Toxicity of antifouling biocides to the intertidal harpacticoid copepod Tigriopus japonicus (Crustacea,Copepoda):Effects of temperature and salinity[J].Marine Pollution Bulletin,2005,51(8):830-837.
[15] 梁永莉,季宏兵,朱毅.莱茵衣藻的双酚A毒性效应及生物富集和降解作用研究[J].环境科学与技术,2014,37(6):25-31,96.Liang Y L,Ji H B,Zhu Y.Toxic effect for bisphenol A to Chlamydomonas reinhardtii and its bioaccumulation and biodegradation[J].Environmental Science & Technology,2014,37(6):25-31,96.
[16] 张普青.巢湖鱼体内四溴双酚A与多溴二苯醚暴露水平的研究[D].哈尔滨:东北林业大学,2011.Zhang P Q.The Research of Exposure Level of Tetrabromobisphenol-A and Polybrominated Diphenyl Ethers in the Chaohu Lake Fish [D].Haerbin:Northeast Forestry University,2011.
[17] Shi H,Wang X,Yi L,et al.Electron paramagnetic resonance evidence of hydroxyl radical generation and oxidative damage induced by tetrabromobisphenol-A in Carassius auratus[J].Aquatic Toxicology,2005,74(4):365-371.
[18] WHO.Environmental Health Criteria 172:Tetrabromobisphenol A and Derivatives[S].Geneva,Switzerland:International Programme on Chemical Safety,1995,172:25-47.
[19] 国家环境保护总局.HJ/T153-2004新化学物质危害评估导则[S].北京:中华人民共和国环境保护部,2004.State Environmental Protection Administration.HJ/T153-2004 New Chemical Hazard Assessment Guidelines[S].Beijing:Ministry of Ecology and Environment of the People's Republic of China,2004
[20] 韩萃,朱丽岩,江田田,等.不同温度盐度下TBBPA对拟长腹剑水蚤的毒性效应[J].中国海洋大学学报(自然科学版),2018,48(1):49-56.HAN C,ZHU L Y,JIANU T T,et al.The effect of TBBPA on copepod(Oithona similis) under different salinity and temperature [J].Periodical of Ocean University of China,2018,48(1):49-56.
[21] 徐风风,朱丽岩,巩文静,等.四溴联苯醚(BDE- 47)对两种海洋桡足类动物的毒性效应[J].生态毒理学报,2013,8(5):737-747.Xu F F,Zhu L Y,Gong W J,et al.Toxic effects of BDE-47 on two marine copepods [J].Asian Journal of Ecotoxicology,2013,8(5):737-747.
[22] Rajendran N,Venugopalan V K.Toxicity of organochlorine pesticides to zooplankton of Vellar Estuary[J].Indian Journal of Marine Science,1988,17(2):168-169.
[23] Peterson W T.Patterns in stage duration and development among marine and freshwater calanoid and cyclopoid copepods:A review of rules,physiological constraints,and evolutionary significance[J].Hydrobiologia,2001,453-454(1):91-105.
[24] 林汝榕,李少菁.铜、镉对中华哲水蚤氨基酸含量影响的实验研究[J].海洋与湖沼,1991,22(3):242-248.Lin R R,Li S J.Effects of copper and cadmium on amino acids in Calanus sinicus[J].Chinese Journal of Oceanology and Limnology,1991,22(3):242-248.
[25] 王晓敏,朱丽岩,陈学超,等.六溴环十二烷对拟长腹剑水蚤的急性毒性和生殖发育毒性效应[J].中国海洋大学学报(自然科学版),2017,47(1):82-88.Wang X M,Zhu L Y,Chen X C,et al.Toxic Effect of HBCD on the acute toxicity,development and reproduction of Oithona Similes[J].Periodical of Ocean University of China,2017,47(1):82-88.
[26] 巩文静.四溴双酚A(TBBPA)对河口常见桡足类的毒性效应及其在中国北方海域的污染分布状况[D].青岛:中国海洋大学,2016.Gong W J.Toxic Effects of Tetrabromobisphenol A (TBBPA) on Copepods in the Estuary and Its Pollution Distribution in Northern China[D].Qingdao:Ocean University of China,2016.
[27] 郑重.浮游生物学概论[M].北京:科学出版社,1965.Zheng Z.Essential of Planktology[M].Beijing:Science Press,1965.
[28] Davies R,Zou E.Polybrominated diphenyl ethers disrupt molting in neonatal Daphnia magna[J].Ecotoxicology,2012,21(5):137-138.
[29] Wollenberger L,Dinan L,Breitholtz M.Brominated flame retardants:Activities in a crustacean development test and in an ecdysteroid screening assay[J].Environmental Toxicology and Chemistry,2005,24(2):400-407
[30] Nakajima A,Saigusa D,Tetsu N,et al.Neurobehavioral effects of tetrabromobisphenol A,a brominated flame retardant,in mice[J].Toxicology Letters,2009,189(1):78-83.
[31] Andesen H R,Hailing Sorensen B,Kusk K O.A parameter for detecting estrogenic exposure in the copepod Acartia tonsa[J].Ecotoxicology and Encironmental Safety,1999,44:56-61.
[32] Walthall W K,Stark J D.A comparison of acute mortality and population growth rate as endpoints of toxicological effect[J].Ecotoxicol Environ Saf,1997,37(1):45-52.
[33] Michalek-Wagner K,Willis B L.Impacts of bleaching on the soft coral Lobophytum compactum.I.Fecundity,fertilization and offspring viability[J].Coral Reefs,2001,19(3):231-239.
[34] 季更生,钱海丰,刘光兴.铜离子对克氏纺锤水蚤影响的初步研究[J].海洋通报,2001,20(1):92-96.Ji G S,Qian H F,Liu G X.Effect of copper ions on Daphnia magna [J].Marine Science Bulletin,2001,20(1):92-96.
[35] Siu W H,Hung C L,Wong H L,et al.Exposure and time dependent DNA strand breakage in hepatopancreas of green-lipped mussels (Perna viridis) exposed to Aroclor 1254,and mixtures of B[a]P and Aroclor 1254[J].Marine Pollution Bulletin,2003,46(10):1285-1293.
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