丙酮酸乙酯联合顺铂作用裸鼠肝移植瘤的研究

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	作者：李超男 ; 许钰云 ; 黄任锦 ; 吴振兴 关键词：顺铂 ; 丙酮酸乙酯 ; 裸鼠 ; 肝癌移植瘤 英文关键词：Cisplatin;;Ethyl Pyruvate;;Nude mice;;Hepatocellular Carcinoma 中文刊名：科学技术创新 英文刊名：Scientific and Technological Innovation 机构：杭州医学院; 出版日期：2019-09-25 出版单位：科学技术创新 年：2019 期：27 基金：浙江省省教育厅科研一般项目（Y201636957）;; 杭州医学院校级博士启动金（2016B04）;; 浙江省大学生科技创新活动计划(新苗人才计划)(2019R425004) 语种：中文; 页：23-26 页数：4 CN：23-1600/N ISSN：2096-4390 分类号：R735.7

摘要

目的:研究丙酮酸乙酯(EP)与顺铂(CDDP)联合处理裸鼠对裸鼠肝癌HepG2移植瘤的作用效果,及对个体的副作用;方法:为裸鼠接种p-Gluc荧光标记的HepG2细胞,分为PBS处理组、CDDP(9.6mM)与EP(60μg)单独注射组、CDDP与EP联合注射组;对各组裸鼠的个体体重、移植瘤体积进行观察测量;结果:药物联合组的肿瘤体积显著小于对照组(*P=0.0146),而CDDP处理组无显著变化。药物联合组与CDDP组中肿瘤抑制率分别为36.8%和62.9%;药物联合组体重平均为21g,CDDP组为19g,EP组与对照组约为25g;结论:EP是CDDP增效剂,药物联合处理有望为肝癌治疗方案提出新的思路,并可能缓解化疗药物单独使用带来的副作用。
        Objective:To study the effect of ethyl pyruvate(EP) combined with cisplatin(CDDP) on HepG2 transplanted tumor in nude mice and the side effects on individuals; Method:Inoculating p-Gluc fluorescently labeled HepG2 cells into nude mice,then divided into PBS-treated group, CDDP(9.6 mM) and EP(60 μg) Separate injection group, and CDDP-EP combined injection group; Observing and measuring individual body weight and transplanted tumor volume of each group; Results:The tumor volume of CDDP-EP therapy group was significantly smaller than that of the control group(*P =0.0146), while there was no significant change in the CDDP therapy group. The tumor inhibition rates in CDDP-EP therapy group and the CDDP group were36.8% and 62.9%, respectively; the average body weight of CDDP-EP therapy group was 21 g, the CDDP group was 19 g, while the EP group and the control group were nearly 25 g;Conclusion:EP is a CDDP synergist, the drug combination therapy is expected to provide new ideas for the treatment of liver cancer, and may alleviate the side effects caused by the separate use of chemotherapy drugs.

引文

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