基于SSR对火炬松×加勒比松杂种形质性状的QLT定位分析
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摘要
对火炬松×加勒比松杂种部分形质性状的QTL进行定位分析,为杂种育种和株型培育提供借鉴。以火炬松1.5代种子园优良单株T1为母本,加勒比松第一代种子园优良单株C1为父本,杂交产生F1代113个单株。使用Join Map?3.0作图软件构建火炬松母本和加勒比松父本的SSR标记遗传图谱,并用MapQTL4.0?作图软件对形质性状的QTL进行定位。火炬松母本图谱总长度293.9 cM,标记间距范围为3.7～47.9 cM,标记平均间距15.5 cM。加勒比松父本图谱总长度245.5 cM,标记间距范围为6.8～49.8 cM,标记平均间距13.6 cM。检测到4个与形质性状有关的QTL位点,其中有2个QTL定位于火炬松染色体LG4上。4个形质性状的QTLs解释方差为8.6%～13.6%,可以利用这些QTLs进行特定株型的培育,如分枝角度的控制培育。
In order to provide a reference for hybrid breeding and plant type breeding, QTL positioning analysis of some form quality traits of Pinus taeda × Pinus caribaea was conducted. A Pinus taeda × P. caribaea F1 population consists of 113 individuals was used to construct linkage maps for both the parents based on SSR makers. The map of female parent spanned 293.9 cM and the distance between loci varied from 3.7 to 47.9 cM,with a average distance of 15.5 cM. The distance between loci varied from 6.8 to 49.8 cM with a total length of245.5 cM and an average maker interval of 13.6 cM in the map of the male parent. Using the MapQTL4.0?, four QTLs for form quality traits were detected, of which two QTLs were located on the chromosome LG4 of Pinus taeda. The QTLs variance explained of the four QTLs for form quality traits was 8.6%-13.6%, which could be used for specific plant type cultivation, such as branch angle control cultivation.
In order to provide a reference for hybrid breeding and plant type breeding, QTL positioning analysis of some form quality traits of Pinus taeda × Pinus caribaea was conducted. A Pinus taeda × P. caribaea F1 population consists of 113 individuals was used to construct linkage maps for both the parents based on SSR makers. The map of female parent spanned 293.9 cM and the distance between loci varied from 3.7 to 47.9 cM,with a average distance of 15.5 cM. The distance between loci varied from 6.8 to 49.8 cM with a total length of245.5 cM and an average maker interval of 13.6 cM in the map of the male parent. Using the MapQTL4.0?, four QTLs for form quality traits were detected, of which two QTLs were located on the chromosome LG4 of Pinus taeda. The QTLs variance explained of the four QTLs for form quality traits was 8.6%-13.6%, which could be used for specific plant type cultivation, such as branch angle control cultivation.
引文
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[14]郭利建,王竹林,汪世娟,等.基于SRAP和SSR标记的小麦品质相关性状的QTL定位[J].麦类作物学报,2016,36(10):1275-1282.
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[20]方宣钧.作物DNA标记辅助育种[M].北京:科学出版社,2001.
[21]马明,杨克强,郭起荣,等.改良CTAB法提取林木树种基因组DNA的研究[J].生物技术,2007,17(3):36-38.
[22]ZHOU Y,BUI T,AUCKLAND L D,et al.Direct fluorescent primers are superior to M13-tailed primers for Pinus taeda microsatellites[J].Biotechniques,2002,32(1):46-52.
[23]VAN OOIJEN J W,VOORRIPS R E.Software for the calculation of genetic linkage maps[BD/OL].Join Map3.0.Wageningen,the Netherlands:Plant Research International,2001.
[24]VAN OOIJEN J W V,BOER M P,JANSEN R C,et al.Map QTL 4.0:Software for the calculation of QTL positions on genetic maps(user manual)[J].Order-a Journal on the Theory of Ordered Sets&Its Applications,2002,501(12):2412.
[25]VOORRIPS R E.Map Chart:software for the graphical presentation of linkage maps and QTLs[J].Journal of Heredity,2002,93(1):77-78.
[26]ZHANG Y X,WANG L H,XIN H G,et al.Construction of a high-density genetic map for sesame based on large scale marker development by specific length amplified fragment(SLAF)sequencing[J].BMC Plant Biol,2013,13(1):141.
[27]ECHT C S,SAHA S,KRUTOVSKY K V,et al.An annotated genetic map of loblolly pine based on microsatellite and c DNA markers[J].BMC Genet,2011,12(1):1-16.
[28]李义良,赵奋成,张应中,等.分子标记在松树遗传与进化研究中的应用[J].分子植物育种,2009,7(5):1004-1009.
[29]黄少伟.松树分子标记辅助育种研究进展[J].林业科学研究,2006,19(6):799-806.
[2]ACHERéV,FAIVRE-RAMPANT P,JEANDROZ S,et al.A full saturated linkage map of Picea abies including AFLP,SSR,ESTP,5S r DNA and morphological markers[J].Theor Appl Genet,2004,108(8):1602-1613.
[3]GOSSELIN I,ZHOU Y,BOUSQUET J,et al.Megagametophyte-derived linkage maps of white spruce(Picea glauca)based on RAPD,SCAR and ESTP markers[J].Theor Appl Genet,2002,104(6/7):987-997.
[4]黄秦军,苏晓华,张香华.利用AFLP和SSR标记构建美洲黑杨×青杨遗传图谱[J].林业科学研究,2004,17(3):291-299.
[5]林艳,黄少伟,毛积鹏,等.湿地松×加勒比松杂种木材密度QTL定位分析[J].江西农业大学学报,2018,40(1):109-117.
[6]宋婉,陈晓阳,续九如,等.林木遗传连锁图谱构建研究进展与发展方向[J].遗传,2003,25(6):749-756.
[7]DEVEY M E,CARSON S D,NOLAN M F,et al.QTLassociations for density and diameter in Pinus radiata and the potential for marker-aided selection[J].Theor Appl Genet,2004,108(3):516-524.
[8]贯春雨.日本落叶松×兴安落叶松RAPD、SSR遗传图谱构建与QTL定位[D].哈尔滨:东北林业大学,2010.
[9]刘龙超,周云,贺苗苗,等.四倍体马铃薯SSR遗传图谱的构建及晚疫病抗性QTL初步定位[J].植物病理学报,2016,46(1):84-90.
[10]LIND M,K?LLMAN T,CHEN J,et al.A Picea abies linkage map based on SNP markers identifies QTLs for four aspects of resistance to Heterobasidion parviporum infection[J].PLo S One,2014,9(7):e101049.
[11]HAYASHI E,KONDO T,TERADA K,et al.Identification of AFLP markers linked to a resistance gene against pine needle gall midge in Japanese black pine[J].Theor Appl Genet,2004,108(6):1177-1181.
[12]KAYA H B,CETIN O,KAYA H S,et al.Association mapping in turkish olive cultivars revealed significant markers related to some important agronomic traits[J].Biochem Genet,2016,54(4):506-533.
[13]韩明丽,刘永立,郑小艳,等.梨遗传连锁图谱的构建及部分果实性状QTL的定位[J].果树学报,2010,27(4):496-503.
[14]郭利建,王竹林,汪世娟,等.基于SRAP和SSR标记的小麦品质相关性状的QTL定位[J].麦类作物学报,2016,36(10):1275-1282.
[15]刘金义,崔海荣,王龙,等.梨果实酸/低酸性状的SSR分析[J].果树学报,2011,28(3):389-393.
[16]李娟,郭斌,安新民,等.欧美杨与藏川杨杂交子代苗期性状QTLs定位分析[J].西南林业大学学报,2016,36(5):10-15.
[17]贺丹,王晓娇,刘阳,等.紫薇几个表型性状的QTLs定位[J].东北林业大学学报,2014,42(7):65-68.
[18]蔡长福,刘改秀,成仿云,等.牡丹遗传作图最适F1分离群体的选择[J].北京林业大学学报,2015,37(3):139-147.
[19]王明庥.林木遗传育种学[M].北京:中国林业出版社,2001.
[20]方宣钧.作物DNA标记辅助育种[M].北京:科学出版社,2001.
[21]马明,杨克强,郭起荣,等.改良CTAB法提取林木树种基因组DNA的研究[J].生物技术,2007,17(3):36-38.
[22]ZHOU Y,BUI T,AUCKLAND L D,et al.Direct fluorescent primers are superior to M13-tailed primers for Pinus taeda microsatellites[J].Biotechniques,2002,32(1):46-52.
[23]VAN OOIJEN J W,VOORRIPS R E.Software for the calculation of genetic linkage maps[BD/OL].Join Map3.0.Wageningen,the Netherlands:Plant Research International,2001.
[24]VAN OOIJEN J W V,BOER M P,JANSEN R C,et al.Map QTL 4.0:Software for the calculation of QTL positions on genetic maps(user manual)[J].Order-a Journal on the Theory of Ordered Sets&Its Applications,2002,501(12):2412.
[25]VOORRIPS R E.Map Chart:software for the graphical presentation of linkage maps and QTLs[J].Journal of Heredity,2002,93(1):77-78.
[26]ZHANG Y X,WANG L H,XIN H G,et al.Construction of a high-density genetic map for sesame based on large scale marker development by specific length amplified fragment(SLAF)sequencing[J].BMC Plant Biol,2013,13(1):141.
[27]ECHT C S,SAHA S,KRUTOVSKY K V,et al.An annotated genetic map of loblolly pine based on microsatellite and c DNA markers[J].BMC Genet,2011,12(1):1-16.
[28]李义良,赵奋成,张应中,等.分子标记在松树遗传与进化研究中的应用[J].分子植物育种,2009,7(5):1004-1009.
[29]黄少伟.松树分子标记辅助育种研究进展[J].林业科学研究,2006,19(6):799-806.
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