黑木耳凝集素的分离、纯化及抗肿瘤活性研究
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摘要
黑木耳是常见的食用菌,不仅营养丰富,还具有众多的生物活性。近年来对黑木耳多糖研究较多,对于其他成分研究较少,因此,本文利用(NH_4)_2SO_4沉淀和亲和层析的方法提取到黑木耳凝集素,对其性质和抗肿瘤活性进行了初步探讨。结果表明,黑木耳凝集素耐酸,不耐碱,不具备热稳定性,具有糖特异性;对肺癌(A549)和乳腺癌细胞(MCF-7)都有抑制性。此研究为今后黑木耳凝集素的活性研究提供基础。
Auricularia auricula is a common edible fungus. Auricularia auricula is rich in a variety of nutritional components and physiological active ingredients. In recent years, there have been many studies on polysaccharides from Auricularia auricula, and there are few studies on other components. Therefore,(NH_4)_2SO_(4 ) precipitation and affinity chromatography were used to extract Auricularia auricula lectin, the properties of Auricularia auricula lectin and the antitumor activity were initially discussed. The results showed that the Auricularia auricula lectin was acid-resistant, alkali-resistant, non-thermally stable, and sugar-specific; it was inhibited against lung cancer(A549) and breast cancer cells(MCF-7). It will provide a basis for future research on the activity of Auricularia auricula lectin.
Auricularia auricula is a common edible fungus. Auricularia auricula is rich in a variety of nutritional components and physiological active ingredients. In recent years, there have been many studies on polysaccharides from Auricularia auricula, and there are few studies on other components. Therefore,(NH_4)_2SO_(4 ) precipitation and affinity chromatography were used to extract Auricularia auricula lectin, the properties of Auricularia auricula lectin and the antitumor activity were initially discussed. The results showed that the Auricularia auricula lectin was acid-resistant, alkali-resistant, non-thermally stable, and sugar-specific; it was inhibited against lung cancer(A549) and breast cancer cells(MCF-7). It will provide a basis for future research on the activity of Auricularia auricula lectin.
引文
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[14] PRASANNA V K,VENKATESH Y P.Characterization of onion lectin (Allium cepa agglutinin) as an immunommodulatory protein inducing Th1-type immune response in vitro [J].International Immunopharmacology,2015,26(2):304-313.
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[19] 杨雅轩,田勇,唐宇,等.食用菌功能活性蛋白通过TLRs信号通路的免疫调节作用研究进展 [J].食品科学,2017,38(19):278-284.
[20] 黄海涛,李楠,柯国萍,等.内源凝集素的肿瘤靶向性研究进展 [J].华西药学杂志,2012,27(3):339-341.
[21] SOUZA M A,CARVALHO F C,RUAS L P,et al.The immunomodulatory effect of plant lectins:a review with emphasis on ArtinM properties [J].Glycoconjugate Journal,2013,30(7):641-657.
[22] LIU Z,LUO Y,ZHOU T T,et al.Could plant lectins become promising anti-tumour drugs for causing autophagic cell death [J].Cell Proliferation,2013,46(5):509-515.
[23] SUN Y Y,LIU L,LI J.Three novel B-type mannose-specific lectins of Cynoglossus semilaevis possess varied antibacterial activities against Gram-negative and Gram-positive bacteria [J].Developmental and Comparative Immunology,2015,55(2):194-202.
[24] GONDIM A C S,ROMERO I,NASCIMENTO K S,et al.A potent anticancer metallo-lectin from dioclea lasiocarpa [J].Journal of Biological Inorganic Chemistry,2017,22(7):179-189.
[25] LACERDA R R E,DO NASCIMENTO E S,DE LACERDA J T J G,et al.Lectin from seeds of a Brazilian lima bean variety (Phaseolus lunatus L.var.cascavel) presents antioxidant,antitumour and gastroprotective activities [J].International Journal of Biological Macromolecules,2017,95(2):1072-1081.
[26] LIAO J H,CHIEN C H,WU H Y,et al.A multivalent marine lectin from Crenomytilus grayanus possesses anti-cancer activity through recognizing globotriose Gb3 [J].Journal of the American Chemical Society,2016,138(14):4787-4795.
[2] CHEN G,LUO Y C,JI B P,et al.Effect of polysaccharide from Auricularia auricula on blood lipid metabolism and lipoprotein lipase activity of ICR mice fed a cholesterol-enriched diet [J].Journal of Food Science,2008,73(6):H103-H108.
[3] OHIRI R C,BASSEY E E.Evaluation and characterization of nutritive properties of the jelly ear culinary-medicinal mushroom Auricularia auricula-judae (Agaricomycetes) from Nigeria [J].International Journal of Medicinal Mushrooms,2017,19(2):173-177.
[4] SUN S,ZHANG X,CHEN W,et al.Production of natural edible melanin by Auricularia auricula and its physicochemical properties [J].Food Chemistry,2016,196:486-492.
[5] DA SILVA P M,DE MOURA M C,GOMES F S,et al.PgTeL,the lectin found in Punica granatum juice,is an antifungal agent against Candida albicans and Candida krusei [J].International Journal of Biological Macromolecules,2018,108(3):391-400.
[6] 刘主,肖仔君,何智丹,等.草菇凝集素 VL-2的分离纯化及其凝血活性研究 [J].江苏农业科学,2015,43(5):273-275.
[7] 杨爱霞,李应彪,许程剑.阿魏菇凝集素分离纯化及理化性质的研究 [J].食品与生物技术学报,2014,33(1):34-40.
[8] 张迪,林勇,杨晓钦,等.双孢蘑菇2796凝集素的提取及其部分理化性质研究 [J].福建农业学报,2011,26(3):457-461.
[9] 陈建华,孙捷,吕福娇,等.桑黄发酵液中凝集素SHL24的分离与生物活性研究 [J].微生物学通报,2018,45(2):420-427.
[10] 尹昭晔,孔炯炜,刘湘,等.柳蘑的化学成分及药理作用研究进展 [J].食用菌,2019,41(1):1-3.
[11] CHAKKERE SHIVAMADHU M,SRINIVAS B K,JAYARAMA S,et al.Anti-cancer and anti-angiogenic effects of partially purified lectin from Praecitrullus fistulosus fruit on in vitro and in vivo model [J].Biomedicing & Pharmacotherapy,2017,96(11):1299-1309.
[12] LACERDA R R E,DO NASCIMENTO E S,DE LACERDA J T J G,et al.Lectin from seeds of a Brazilian lima bean variety (Phaseolus lunatus L.var.cascavel) presents antioxidant,antitumour and gastroprotective activities [J].International Journal of Biological Macromolecules,2017,95(2):1072-1081.
[13] GONDIM A C S,ROMERO-CANELON I,SOUSA E H S,et al.The potent anti-cancer activity of Dioclea lasiocarpa lectin [J].Journal of Inorganic Biochemistry,2017,175(7):179-189.
[14] PRASANNA V K,VENKATESH Y P.Characterization of onion lectin (Allium cepa agglutinin) as an immunommodulatory protein inducing Th1-type immune response in vitro [J].International Immunopharmacology,2015,26(2):304-313.
[15] LI Y R,ZHANG G Q,NG T B,et al.A novel lectin with antiproliferative and HIV-1 reverse transcriptase inhibitory activities from dried fruiting bodies of the monkey head mushroom Hericium erinaceum [J].Journal of Biomedicine and Biotechnology,2010,2010:716515.
[16] 孙慧,赵辰光,仝鑫,等.一种新杨树菇(Agrocybe aegerita)凝集素的纯化及生化特性 [J].中国生物化学与分子生物学报,2003,19(1):96-102.
[17] 张国庆,陈青君,赵爽,等.红菇属两种凝集素的分离纯化与比较研究 [J].菌物学报,2012,31(1):110-118.
[18] 林玉满,苏爱华.牛舌菌凝集素的分离、性质及细胞凝集活性 [J].天然产物研究与开发,2004,16 (5):420-425.
[19] 杨雅轩,田勇,唐宇,等.食用菌功能活性蛋白通过TLRs信号通路的免疫调节作用研究进展 [J].食品科学,2017,38(19):278-284.
[20] 黄海涛,李楠,柯国萍,等.内源凝集素的肿瘤靶向性研究进展 [J].华西药学杂志,2012,27(3):339-341.
[21] SOUZA M A,CARVALHO F C,RUAS L P,et al.The immunomodulatory effect of plant lectins:a review with emphasis on ArtinM properties [J].Glycoconjugate Journal,2013,30(7):641-657.
[22] LIU Z,LUO Y,ZHOU T T,et al.Could plant lectins become promising anti-tumour drugs for causing autophagic cell death [J].Cell Proliferation,2013,46(5):509-515.
[23] SUN Y Y,LIU L,LI J.Three novel B-type mannose-specific lectins of Cynoglossus semilaevis possess varied antibacterial activities against Gram-negative and Gram-positive bacteria [J].Developmental and Comparative Immunology,2015,55(2):194-202.
[24] GONDIM A C S,ROMERO I,NASCIMENTO K S,et al.A potent anticancer metallo-lectin from dioclea lasiocarpa [J].Journal of Biological Inorganic Chemistry,2017,22(7):179-189.
[25] LACERDA R R E,DO NASCIMENTO E S,DE LACERDA J T J G,et al.Lectin from seeds of a Brazilian lima bean variety (Phaseolus lunatus L.var.cascavel) presents antioxidant,antitumour and gastroprotective activities [J].International Journal of Biological Macromolecules,2017,95(2):1072-1081.
[26] LIAO J H,CHIEN C H,WU H Y,et al.A multivalent marine lectin from Crenomytilus grayanus possesses anti-cancer activity through recognizing globotriose Gb3 [J].Journal of the American Chemical Society,2016,138(14):4787-4795.
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