质子和太空辐射对金鱼草的诱变效应
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摘要
以金鱼草为试材,采用太空诱变和质子辐射的方式,研究了辐射对金鱼草表观性状和分子水平的变化,以生物学统计的方法对金鱼草发芽率、株高等数据进行分析。用RAPD和Qpcrd方法对金鱼草基因组DNA和花青素调控基因Del和Rosea的表达量进行研究。结果表明:2种诱变方式使红色金鱼草发芽率显著降低,黄色金鱼草发芽率基本不变;对株高影响不显著;叶片由对照的矩圆状变为太空诱变后的长椭圆形和质子辐射的披针状;RAPD结果显示太空诱变后产生的多态性比率大于质子辐射,黄色金鱼草的多态性比率稍高于红色金鱼草;qPCR结果显示Del基因和Rosea基因在红色金鱼草中的表达量太空诱变和质子辐射后都高于对照,且太空诱变对红色金鱼草基因的相对表达量高于质子辐射,Del基因和Rosea基因在辐射后黄色鱼草中的表达量都低于对照。
The effects of space mutation and proton radiation on the apparent properties and molecular level of Antirrhinum majus were studied.The germination rate and plant height of Antirrhinum majus were analyzed by biological statistics.RAPD and Qpcr methods were used to study the expression of genomic DNA and anthocyanin regulatory genes Del and Rosea in Antirrhinum majus.The results showed that the two mutagenesis methods significantly reduced the germination rate of red Antirrhinum majus,while the germination rate of yellow Antirrhinum majus remained basically unchanged,and had no significant effect on plant height.The blades changed from the control rectangular circle to the long oval shape and the Antirrhinum majus shape of proton radiation after space mutation.The results showed that the rate of polymorphism produced by space mutation was higher than that of proton radiation,and the polymorphism rate of yellow croaker grass was slightly higher than that of red Antirrhinum majus.The results of qPCR showed that the expression levels of Del gene and Rosea gene were higher than those of the control after space mutation and proton radiation,and the relative expression of Del gene and Rosea gene was higher than that of proton radiation.The expression levels of Del gene and Rosea gene were lower than those of the control after space mutation.
The effects of space mutation and proton radiation on the apparent properties and molecular level of Antirrhinum majus were studied.The germination rate and plant height of Antirrhinum majus were analyzed by biological statistics.RAPD and Qpcr methods were used to study the expression of genomic DNA and anthocyanin regulatory genes Del and Rosea in Antirrhinum majus.The results showed that the two mutagenesis methods significantly reduced the germination rate of red Antirrhinum majus,while the germination rate of yellow Antirrhinum majus remained basically unchanged,and had no significant effect on plant height.The blades changed from the control rectangular circle to the long oval shape and the Antirrhinum majus shape of proton radiation after space mutation.The results showed that the rate of polymorphism produced by space mutation was higher than that of proton radiation,and the polymorphism rate of yellow croaker grass was slightly higher than that of red Antirrhinum majus.The results of qPCR showed that the expression levels of Del gene and Rosea gene were higher than those of the control after space mutation and proton radiation,and the relative expression of Del gene and Rosea gene was higher than that of proton radiation.The expression levels of Del gene and Rosea gene were lower than those of the control after space mutation.
引文
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[23] 李谨,耿金鹏,曹天光,等.太空环境对仙客来诱变效应的研究[J].北方园艺,2015(4):112-115.
[24] 白昌军,刘国道,姚庆群,等.太空搭载‘热研2号’柱花草后代RAPD多态性分析[J].草地学报,2008,16(4):336-340.
[25] 许志茹,李春雷,崔国新,等.植物花青素合成中的MYB蛋白[J].植物生理学报,2008,44(3):597-604.
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[2] 聂鑫,陈国平,张利,等.花青素合成转录因子表达载体的构建及遗传转化油菜研究[J].广东农业科学,2014,41(11):149-153.
[3] 李多芳,董亚净,耿金鹏,等.快中子辐射百日草当代的生物学效应[J].北方园艺,2015(12):58-62.
[4] 李谨.太空电离辐射诱发植物变异的初步研究[D].天津:河北工业大学,2016.
[5] 李佳,周瑜,张启明,等.质子辐照对金花葵生长发育的影响及其基因组变异的RAPD分析[J].广东农业科学,2015,42(14):41-47.
[6] 王雁,李潞滨,韩蕾.空间诱变技术及其在我国花卉育种上的应用[J].林业科学研究,2002,15(2):229-234.
[7] 梅再胜,柯晓华,王维.黄石市太空莲36号子莲高产栽培技术[J].长江蔬菜,2016(23):34-35.
[8] 梁祖培,张燕,熊波,等.天然植物中原花青素提取和纯化方法研究进展[J].食品安全质量检测学报,2017,8(8):3029-3036.
[9] 梁立军,杨祎辰,王二欢,等.植物花青素生物合成与调控研究进展[J].安徽农业科学,2018,46(21):18-24.
[10] 亓燕红.彩色玉米籽粒中参与花青素合成基因的表达分析[D].泰安:山东农业大学,2011.
[11] 孙婧文,吕立堂,赵德刚.花青素合成转录因子Vlmyb2遗传转化烟草的研究[J].山地农业生物学报,2018,37(1):7-11.
[12] 马文瑞,邹弯,魏玉洁,等.酿酒葡萄品种SSR-PCR体系的优化与建立[J].食品与发酵工业,2018,44(3):52-57.
[13] 吴福川,胡秀,郑思乡.不同倍性金鱼草基因组DNA随机扩增多态性研究[J].云南农业大学学报(自然科学版),2005(4):482-485.
[14] 阳成波,印遇龙,黄瑞林,等.实时定量RT-PCR的原理及方法[J].免疫学杂志,2003(s1):145-150.
[15] RAJEEVAN M S,RANAMUKHAARACHCHI D G,VERNON S D,et al.Use of real-time quantitative PCR to validate the results of cDNA array and differential display PCR technologies[J].Methods,2001,25(4):443-451.
[16] 罗文龙,陈立凯,王慧,等.“神舟八号”搭载‘航恢173’种子的当代生物效应及SSR分析[J].中国农学通报,2014,30(15):11-16.
[17] 张晨冰.航空搭载对薰衣草种子萌发的影响[J].安徽农业科学,2017,45(3):35-37.
[18] 李谨,耿金鹏,曹天光,等.太空环境对四季薰衣草的诱变效应[J].生物物理学报,2014,30(3):207-215.
[19] 李多芳,田安然,耿金鹏,等.航天搭载诱导百日草的生物学效应[J].北方园艺,2015(23):97-100.
[20] 钱林庆,周云龙,孙凡捷,等.质子束辐射金花葵种子和花粉的生物学效应[J].中国农业科技导报,2015,17(6):37-43.
[21] 郭建秋,吴存祥,冷建田,等.质子束诱变大豆的能量和剂量效应[J].核农学报,2009,23(1):49-53.
[22] 薛淮,刘敏,张纯花,等.卫星搭载的花卉RAPD分子检测[J].北京园林,2002(3):31-32.
[23] 李谨,耿金鹏,曹天光,等.太空环境对仙客来诱变效应的研究[J].北方园艺,2015(4):112-115.
[24] 白昌军,刘国道,姚庆群,等.太空搭载‘热研2号’柱花草后代RAPD多态性分析[J].草地学报,2008,16(4):336-340.
[25] 许志茹,李春雷,崔国新,等.植物花青素合成中的MYB蛋白[J].植物生理学报,2008,44(3):597-604.
[26] SCHWINN K,VENAIL J,SHANG Y,et al.A small family of MYB-regulatory genes controls floral pigmentation intensity and patterning in the genus Antirrhinum[J].Plant Cell,2006,18(4):831-851.
[27] ALMEIDA J,CARPENTER R,ROBBINS T P,et al.Genetic interactions underlying flower color patterns in Antirrhinum majus[J].Genes Dev,1989,3(11):1758-1767.
[28] CATHIE M,ROSEMARY C C,HANS S,et al.Molecular analysis of instability in flower pigmentation of Antirrhinum majus,following isolation of the pallida locus by transposon tagging[J].Embo Journal,1985,4(7):1625-1630.
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