不同碳氮源对Paracoccus sp.QD15-1降解邻苯二甲酸二甲酯的影响
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摘要
邻苯二甲酸二甲酯(Dimethyl phthalate, DMP)是一种高毒性的有机污染物,Paracoccus sp.QD15-1能够高效降解DMP,碳氮源是微生物需求量最大的生长要素。本实验以Paracoccus sp.QD15-1为研究对象,在基础无机盐(MSM)培养基中分别外加不同碳氮源(碳源分别为5 g·L~(-1)的葡萄糖、蔗糖、乳糖和淀粉;氮源分别为5 g·L~(-1)的硫酸铵、硝酸钠、硝酸铵和尿素),研究Paracoccus sp.QD15-1生长和降解DMP能力的变化。结果表明,外加4种碳源后,菌株生物量和降解DMP能力均显著提高,其中外加淀粉处理的最大比生长率达到0.13 lg(cfu)·h~(-1),36 h降解率为78.2%,DMP半衰期为15.6 h。外加4种氮源后,降解菌的生物量均有所上升,其中外加尿素,36 h降解率为62.8%。研究表明,Paracoccus sp.QD15-1在不同碳氮源中均能够较好地降解DMP,且外加淀粉作为碳源和外加尿素作为氮源的降解效果更佳,在实际工程应用中具有良好前景。
Dimethyl phthalate(DMP)is a highly toxic organic pollutant. Paracoccus sp.QD15-1 can degrade DMP efficiently. The carbon and nitrogen sources are the important nutrients for the growth of microorganisms. In this study, the growth and DMP degradation of Paracoccus sp. QD15-1 were detected in the basic inorganic salt medium by addition of different carbon and nitrogen sources. The results showed that the growth and capacity for degrading DMP of Paracoccus sp.QD15-1 were significantly improved by four carbon sources. With starch addition, the maximum specific growth rate reached to 0.13 lg(cfu)·d~(-1), the degrading rate of DMP reached 78.2%, and half-life of DMP reached at 15.6 h. The biomass of Paracoccus sp.QD15-1 was improved significantly by the addition of nitrogen sources. With urea addition, the degrading rate of DMP reached 62.8% at 36 h. Therefore, the addition of carbon and nitrogen sources could improve the capacity of Paracoccus sp.QD15-1 for degrading DMP, and Paracoccus sp.QD15-1 would have good prospects in practical engineering applications.
Dimethyl phthalate(DMP)is a highly toxic organic pollutant. Paracoccus sp.QD15-1 can degrade DMP efficiently. The carbon and nitrogen sources are the important nutrients for the growth of microorganisms. In this study, the growth and DMP degradation of Paracoccus sp. QD15-1 were detected in the basic inorganic salt medium by addition of different carbon and nitrogen sources. The results showed that the growth and capacity for degrading DMP of Paracoccus sp.QD15-1 were significantly improved by four carbon sources. With starch addition, the maximum specific growth rate reached to 0.13 lg(cfu)·d~(-1), the degrading rate of DMP reached 78.2%, and half-life of DMP reached at 15.6 h. The biomass of Paracoccus sp.QD15-1 was improved significantly by the addition of nitrogen sources. With urea addition, the degrading rate of DMP reached 62.8% at 36 h. Therefore, the addition of carbon and nitrogen sources could improve the capacity of Paracoccus sp.QD15-1 for degrading DMP, and Paracoccus sp.QD15-1 would have good prospects in practical engineering applications.
引文
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[16]岳黎,唐赟,宋嫣,等.不同碳、氮源对苯酚降解菌XH-10的生长和苯酚降解的影响[J].西华师范大学学报(自然科学版),2012,33(2):146-153.YUE Li,TANG Yun,SONG Yan,et al.Effects of different carbon and nitrogen sources on growth and phenol degradation of phenol degrading bacterium XH-10[J].Journal of China West Normal University(Natural Science Edition),2012,33(2):146-153.
[17]冉晓频.土壤/污泥中邻苯二甲酸酯类化合物的研究进展[J].广州化工,2017,45(15):27-29.RAN Xiao-pin.Research progress on phthalic acid esters(PAEs)in soil,sludges and sediments[J].Guangzhou Chemical Industry,2017,45(15):27-29.
[18]王凯荣,崔明明.农业土壤中邻苯二甲酸酯污染研究进展[J].应用生态学报,2013,24(9):2699-2708.WANG Kai-rong,CUI Ming-ming.Phthalic acid esters(PAEs)pollution in farmland soils:A review[J].Chinese Journal of Applied Ecology,2013,24(9):2699-2708.
[19]Wang J,Luo Y,Teng Y,et al.Soil contamination by phthalate esters in Chinese intensive vegetable production systems with different modes of use of plastic film[J].Environmental Pollution,2013,180(3):265-273.
[20]Niu L,Yang X,Chao X,et al.Status of phthalate esters contamination in agricultural soils across China and associated health risks[J].Environmental Pollution,2014,195:16-23.
[21]刘丹丹,刘畅,刘长风,等.降解菌Pseudomonas sp.对阿特拉津的降解条件优化[J].贵州农业科学,2016,44(10):46-49.LIU Dan-dan,LIU Chang,LIU Chang-feng,et al.Optimization for degradation conditions of Pseudomonas sp.strain with degrading atrazine ability[J].Guizhou Agricultural Sciences,2016,44(10):46-49.
[22]张欢,柴立元,朱咏华,等.3株细菌降解木质素的条件调控研究[J].中南大学学报(自然科学版),2011,42(10):2922-2928.ZHANG Huan,CHAI Li-yuan,ZHU Yong-hua,et al.Cultural condition regulations for three lignin degrading bacteria[J].Journal of Central South University(Science and Technology),2011,42(10):2922-2928.
[23]Jin L,Sun X M,Zhang X J,et al.Co-metabolic biodegradation of DBP by Paenibacillus sp.S-3 and H-2[J].Current Microbiology,2014,68(6):708-716.
[2]张惠.邻苯二甲酸二甲酯(DMP)对黄瓜生育期毒性效应研究[D].哈尔滨:东北农业大学,2015.ZHANG Hui.Toxic effect of dimethy phthalate(DMP)on cucumber in growth stage[D].Harbin:Northeast Agricultural University,2015.
[3]Dickson-Spillmann M,Siegrist M,Keller C,et al.Phthalate exposure through food and consumers′risk perception of chemicals in food[J].Risk Anal,2009,29(8):1170-1181.
[4]聂湘平,吴志辉,李凯彬,等.不同暴露方式下酞酸酯在黄斑篮子鱼体中的富集[J].中国环境科学,2007,27(4):467-471.NIE Xiang-ping,WU Zhi-hui,LI Kai-bin,et al.Effects of different exposure ways upon the bioaccumulation of phathalate esters in Siganus oramin[J].Chinese Environmental Science,2007,27(4):467-471.
[5]Kapanen A,Stephen R,Brüggemann J,et al.Diethyl phthalate in compost:Ecotoxicological effects and response of the microbial community[J].Chemosphere,2007,67:2201-2209.
[6]赵晓松,王志刚,王春龙,等.采用光谱学技术分析邻苯二甲酸酯与DNA互作机制[J].生态毒理学报,2017,12(4):385-391.ZHAO Xiao-song,WANG Zhi-gang,WANG Chun-long,et al.Bonding mechanism between phthalic acid esters and DNA by spectroscopy technologies[J].Asian Journal of Ecotoxicology,2017,12(4):385-391.
[7]张宝峰.设施菜地土壤中邻苯二甲酸酯累积及吸附特征研究[D].青岛:青岛农业大学,2014.ZHANG Bao-feng.Research of phthalates′accumulation in greenhouse soil and adsorb characteristics[D].Qingdao:Qingdao Agricultural University,2014.
[8]Han D,Li J,Cao H,et al.Theoretical investigation on the mechanisms and kinetics of OH-initiated photo oxidation of dimethyl phthalate(DMP)in atmosphere[J].Chemosphere,2014,95:50-57.
[9]严佳丽,陈湖星,杨杨,等.一株高效DEHP降解菌的分离、鉴定及其降解特性[J].微生物学通报,2014,41(8):1532-1540.YAN Jia-li,CHEN Hu-xing,YANG Yang,et al.Isolation and characterization of a highly efficient DEHP-degrading bacterium[J].Microbiology,2014,41(8):1532-1540.
[10]李建龙,邵向丽,刘书亮,等.邻苯二甲酸二丁酯降解菌株的筛选鉴定及其降解特性研究[J].现代食品科技,2014(10):108-114.LI Jian-long,SHAO Xiang-li,LIU Shu-liang,et al.Isolation and identification of dibutyl phthalate-degrading strain and its degradation characteristics[J].Modern Food Science and Technology,2014(10):108-114.
[11]刘帅,王志刚,胡云龙,等.一株黑土生境中DMP降解菌的分离鉴定和降解条件研究[J].浙江农业学报,2015,27(10):1807-1812.LIU Shuai,WANG Zhi-gang,HU Yun-long,et al.Identification of a DMP-degraded strain in black soil and the optimation of degradation conditions[J].Acta Agriculturae Zhejiangensis,2015,27(10):1807-1812.
[12]徐伟慧,刘帅,王志刚,等.一株DMP降解菌的分离鉴定及特性研究[J].农业环境科学学报,2018,37(8):1724-1732.XU Wei-hui,LIU Shuai,WANG Zhi-gang,et al.Isolation,identification,and degradation characteristics of a DMP-degrading strain[J].Journal of Agro-Environment Science,2018,37(8):1724-1732.
[13]严佳丽.高效DEHP降解菌的分离鉴定及降解基因的研究[D].武汉:华中师范大学,2015.YAN Jia-li.Isolation and identification of efficient DEHP degrading bacteria and study on degrading genes[D].Wuhan:Huazhong Normal University,2015.
[14]Chun L.Isolation identification and soil remediation of atrazine-degrading strain T3AB1[J].Chemosphere,2010,50(12):1642-1650.
[15]范寰,梁军锋,赵润,等.碳氮源对复合木质素降解菌木质素降解能力及相关酶活的影响[J].农业环境科学学报,2010,29(7):1394-1398.FAN Huan,LIANG Jun-feng,ZHAO Run,et al.Effect of carbon and nitrogen sources on lignin degradation ability and enzyme activity of dual-cultured,lignin-degrading fungi[J].Journal of Agro-Environment Science,2010,29(7):1394-1398.
[16]岳黎,唐赟,宋嫣,等.不同碳、氮源对苯酚降解菌XH-10的生长和苯酚降解的影响[J].西华师范大学学报(自然科学版),2012,33(2):146-153.YUE Li,TANG Yun,SONG Yan,et al.Effects of different carbon and nitrogen sources on growth and phenol degradation of phenol degrading bacterium XH-10[J].Journal of China West Normal University(Natural Science Edition),2012,33(2):146-153.
[17]冉晓频.土壤/污泥中邻苯二甲酸酯类化合物的研究进展[J].广州化工,2017,45(15):27-29.RAN Xiao-pin.Research progress on phthalic acid esters(PAEs)in soil,sludges and sediments[J].Guangzhou Chemical Industry,2017,45(15):27-29.
[18]王凯荣,崔明明.农业土壤中邻苯二甲酸酯污染研究进展[J].应用生态学报,2013,24(9):2699-2708.WANG Kai-rong,CUI Ming-ming.Phthalic acid esters(PAEs)pollution in farmland soils:A review[J].Chinese Journal of Applied Ecology,2013,24(9):2699-2708.
[19]Wang J,Luo Y,Teng Y,et al.Soil contamination by phthalate esters in Chinese intensive vegetable production systems with different modes of use of plastic film[J].Environmental Pollution,2013,180(3):265-273.
[20]Niu L,Yang X,Chao X,et al.Status of phthalate esters contamination in agricultural soils across China and associated health risks[J].Environmental Pollution,2014,195:16-23.
[21]刘丹丹,刘畅,刘长风,等.降解菌Pseudomonas sp.对阿特拉津的降解条件优化[J].贵州农业科学,2016,44(10):46-49.LIU Dan-dan,LIU Chang,LIU Chang-feng,et al.Optimization for degradation conditions of Pseudomonas sp.strain with degrading atrazine ability[J].Guizhou Agricultural Sciences,2016,44(10):46-49.
[22]张欢,柴立元,朱咏华,等.3株细菌降解木质素的条件调控研究[J].中南大学学报(自然科学版),2011,42(10):2922-2928.ZHANG Huan,CHAI Li-yuan,ZHU Yong-hua,et al.Cultural condition regulations for three lignin degrading bacteria[J].Journal of Central South University(Science and Technology),2011,42(10):2922-2928.
[23]Jin L,Sun X M,Zhang X J,et al.Co-metabolic biodegradation of DBP by Paenibacillus sp.S-3 and H-2[J].Current Microbiology,2014,68(6):708-716.
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