模拟喀斯特不同土壤生境胁迫对刺槐幼苗光合特性及干物质分配的影响
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摘要
为探讨刺槐对不同喀斯特环境的适应能力及其在生态系统构建的配置方式,通过室内水培实验,人工模拟干旱、高重碳酸盐、低营养、缺磷4种喀斯特土壤生境胁迫,研究刺槐幼苗的光合特性、碳酸酐酶活力、δ~(13)C值和干物质分配对不同喀斯特生境胁迫的响应及适应策略。结果表明:在干旱环境下,刺槐幼苗短期内(10天)最为敏感且受抑制最强,虽增加根冠比和水分利用效率,长期内(20天)能够维持生存;在高重碳酸盐环境下,因气孔导度降低,其通过骤增碳酸酐酶活力来增加溶液中HCO_3~-利用能力,以此提高茎叶干物质分配,长期保持较好生长力;在低营养环境下,短期内(10天)限制刺槐生长的主要因素为气孔限制,长期内(20天)通过上调碳酸酐酶活力增加对HCO_3~-的利用能力,提高根部干物质分配,但其生长仍受溶液无机元素的浓度限制;在缺磷环境下,刺槐通过上调碳酸酐酶活力来增加HCO_3~-利用能力,提高根部干物质分配,维持较高的生长能力。刺槐幼苗在不同喀斯特环境下表现出不同的适应机制,刺槐对4种模拟的喀斯特土壤环境胁迫适应性综合评价为:重碳酸盐>缺磷>低营养>干旱。
To explore the adaptability of Robinia pseudoacacia to different karst environments and its configuration in ecosystem construction,four kinds of karst soil habitat stresses,including drought,high-carbonate,low-nutrient,and phosphorus-deficiency,were artificially simulated under indoor hydroponic experiment. We measured the photosynthetic characteristics,carbonic anhydrase activity,δ~(13)C value and dry matter allocation of seedlings under different karst habitat stresses. The results showed that under drought environment,the seedlings of R. pseudoacacia were the most sensitive and inhibited in the short term( 10 days). Although the root-shoot ratio and water use efficiency were increased,the seedlings could survive in a longer term( 20 days).Under the high bicarbonate environment,R. pseudoacacia seedlings increased the utilization of HCO_3~- by increasing the activity of carbonic anhydrase due to the decrease of stomatal conductance,thereby improving dry matter allocation to stems and leaves and maintaining normal growth for a longer term. Under low nutrient environment,stomatal factor was the main limitation for the growth of R. pseudoacacia in a short term( 10 days). R. pseudoacacia increased the utilization of HCO_3~- in the solution by increasing the activity of carbonic anhydrase in the longer term( 20 days),and increased dry matter allocation to roots. However,the growth was still limited by the concentration of inorganic elements in the solution. Under phosphorus-deficient environment,the R. pseudoacacia seedlings increased the utilization of HCO_3~- in the solution by up-regulating the activity of carbonic anhydrase,increased dry matter allocation to roots,and maintained the high growth capacity. The adaptation strateiges of R. pseudoacacia seedlings differed under different karst environments. The results of comprehensive evaluation showed that the adaptation of R.pseudoacacia to four simulated karst soil stresses was in the order of high carbonate,phosphorus deficiency,low nutrition,and drought.
To explore the adaptability of Robinia pseudoacacia to different karst environments and its configuration in ecosystem construction,four kinds of karst soil habitat stresses,including drought,high-carbonate,low-nutrient,and phosphorus-deficiency,were artificially simulated under indoor hydroponic experiment. We measured the photosynthetic characteristics,carbonic anhydrase activity,δ~(13)C value and dry matter allocation of seedlings under different karst habitat stresses. The results showed that under drought environment,the seedlings of R. pseudoacacia were the most sensitive and inhibited in the short term( 10 days). Although the root-shoot ratio and water use efficiency were increased,the seedlings could survive in a longer term( 20 days).Under the high bicarbonate environment,R. pseudoacacia seedlings increased the utilization of HCO_3~- by increasing the activity of carbonic anhydrase due to the decrease of stomatal conductance,thereby improving dry matter allocation to stems and leaves and maintaining normal growth for a longer term. Under low nutrient environment,stomatal factor was the main limitation for the growth of R. pseudoacacia in a short term( 10 days). R. pseudoacacia increased the utilization of HCO_3~- in the solution by increasing the activity of carbonic anhydrase in the longer term( 20 days),and increased dry matter allocation to roots. However,the growth was still limited by the concentration of inorganic elements in the solution. Under phosphorus-deficient environment,the R. pseudoacacia seedlings increased the utilization of HCO_3~- in the solution by up-regulating the activity of carbonic anhydrase,increased dry matter allocation to roots,and maintained the high growth capacity. The adaptation strateiges of R. pseudoacacia seedlings differed under different karst environments. The results of comprehensive evaluation showed that the adaptation of R.pseudoacacia to four simulated karst soil stresses was in the order of high carbonate,phosphorus deficiency,low nutrition,and drought.
引文
安显金,李维.2018.基于WOS数据库和CSCD的全球喀斯特研究动态.贵州师范大学学报:自然科学版,36(3):14-22.
杜振宇,张树岩.2012.刺槐抗逆性研究进展.山东林业科技,42(3):116-121.
高小锋,王进鑫,张波,等.2010.不同生长期干旱胁迫对刺槐幼树干物质分配的影响.生态学杂志,29(6):1103-1108.
杭红涛.2015.喀斯特逆境下两种植物的碳酸酐酶基因表达及无机碳利用(博士学位论文).北京:中国科学院大学.
杭红涛,吴沿友,邢德科,等.2018.贵州玉舍国家森林公园三种造林植物光合生理特征研究.广西植物,38(1):36-47.
刘长成,刘玉国,郭柯.2011.四种不同生活型植物幼苗对喀斯特生境干旱的生理生态适应性.植物生态学报,35(10):1070-1082.
刘成刚,吴永波,薛建辉,等.2012.刺槐滇柏混交林及纯林土壤酶与养分相关性研究.土壤通报,43(6):1427-1431.
卢耀如.2010.中国喀斯特.北京:高等教育出版社.
容丽,王世杰,卢红梅.2008.喀斯特峡谷区石漠化过程中土壤的生物地球化学特征.矿物岩石地球化学通报,27(s1):501-503.
王丁,姚健,杨雪,等.2011.干旱胁迫条件下6种喀斯特主要造林树种苗木叶片水势及吸水潜能变化.生态学报,31(8):2216-2226.
王天华,李玫,王沙生,等.1995.不同营养条件下杨槐混交对生长和杨树光合作用的影响.北京林业大学学报,17(4):62-66.
魏兴萍,刘文.2014.当前国际岩溶洞穴学界研究热点-袁道先院士访谈录.重庆师范大学学报:自然科学版,31(1):1-5.
吴沿友.2011.喀斯特适生植物固碳增汇策略.中国岩溶,30(4):461-465.
邢德科,吴沿友,吴沿胜,等.2016.麻疯树和枫杨幼苗对5种模拟喀斯特逆境的光合生理响应.中国岩溶,35(6):649-656.
姚永慧.2014.中国西南喀斯特石漠化研究进展与展望.地理科学进展,33(1):76-84.
郑元,赵忠,周慧,等.2010.刺槐树冠光合作用的空间异质性.生态学报,30(23):6399-6408.
朱东方.2010.刺槐种质资源评价及良种选育(硕士学位论文).泰安:山东农业大学.
Deng B,Du W,Liu C,et al.2012.Antioxidant response to drought,cold and nutrient stress in two ploidy levels of tobacco plants:Low resource requirement confers polytolerance in polyploids.Plant Growth Regulation,66:37-47.
Hang HT,Wu YY.2016.Quantification of photosynthetic inorganic carbon utilisation via a bidirectional stable carbon isotope tracer.Acta Geochimica,35:130-137.
Hu H.2010.Carbonic anhydrases are upstream regulators of CO2-controlled stomatal movements in guard cells.Nature Cell Biology,12:87-93.
Keenan TF,Hollinger DY,Bohrer G,et al.2013.Increase in forest water-use efficiency as atmospheric carbon dioxide concentrations rise.Nature,499:324-327.
Leuning R,Kelliher FM,Pury DGGD,et al.2010.Leaf nitrogen,photosynthesis,conductance and transpiration:Scaling from leaves to canopies.Plant,Cell&Environment,18:1183-1200.
Li H,Cheng Z.2015.Hoagland nutrient solution promotes the growth of cucumber seedlings under light-emitting diode light.Acta Agriculturae Scandinavica,65:74-82.
Li KR,Wang HH,Han G,et al.2008.Effects of brassinolide on the survival,growth and drought resistance of Robinia pseudoacacia seedlings under water-stress.New Forests,35:255-266.
Müller WEG,Qiang L,Schr9der HC,et al.2014.Carbonic anhydrase:A key regulatory and detoxifying enzyme for Karst plants.Planta,239:213-229.
Nie YP,Chen HS,Wang KL,et al.2014.Rooting characteristics of two widely distributed woody plant species growing in different karst habitats of southwest China.Plant Ecology,215:1099-1109.
Perez-Martin A,Michelazzo C,TorresRuiz JM,et al.2014.Regulation of photosynthesis and stomatal and mesophyll conductance under water stress and recovery in olive trees:Correlation with gene expression of carbonic anhydrase and aquaporins.Journal of Experimental Botany,65:3143-3156.
Sensua BM.2015.Spatial and short-temporal variability ofδ13Candδ15N and water-use efficiency in pine needles of the three forests along the most industrialized part of Poland.Water,Air&Soil Pollution,226:1-13.
Turan M,Güllüce M,akmak R,et al.2013.Effect of plant growth-promoting rhizobacteria strain on freezing injury and antioxidant enzyme activity of wheat and barley.Journal of Plant Nutrition,36:731-748.
Wang SJ,Liu QM,Zhang DF.2004.Karst rocky desertification in southwestern China:Geomorphology,landuse,impact and rehabilitation.Land Degradation&Development,15:115-121.
Wang Z,Wang M,Liu L,et al.2013.Physiological and proteomic responses of diploid and tetraploid black locust(Robinia pseudoacacia L.)subjected to salt stress.International Journal of Molecular Sciences,14:20299-20325.
Wang R,Wu YY,Xing DK,et al.2017.Biomass production of three biofuel energy plants’use of a new carbon resource by carbonic anhydrase in simulated karst soils:Mechanism and capacity.Energies,10:1370-1383.
Wu YY,Shi QQ,Wang K,et al.2011.An electrochemical approach coupled with Sb microelectrode to determine the activities of carbonic anhydrase in the plant leaves.Future Intelligent Information Systems,86:87-94.
Yu W,Liu Y,Song L,et al.2016.Effect of differential light quality on morphology,photosynthesis,and antioxidant enzyme activity in Camptotheca acuminata seedlings.Journal of Plant Growth Regulation,36:1-13.
杜振宇,张树岩.2012.刺槐抗逆性研究进展.山东林业科技,42(3):116-121.
高小锋,王进鑫,张波,等.2010.不同生长期干旱胁迫对刺槐幼树干物质分配的影响.生态学杂志,29(6):1103-1108.
杭红涛.2015.喀斯特逆境下两种植物的碳酸酐酶基因表达及无机碳利用(博士学位论文).北京:中国科学院大学.
杭红涛,吴沿友,邢德科,等.2018.贵州玉舍国家森林公园三种造林植物光合生理特征研究.广西植物,38(1):36-47.
刘长成,刘玉国,郭柯.2011.四种不同生活型植物幼苗对喀斯特生境干旱的生理生态适应性.植物生态学报,35(10):1070-1082.
刘成刚,吴永波,薛建辉,等.2012.刺槐滇柏混交林及纯林土壤酶与养分相关性研究.土壤通报,43(6):1427-1431.
卢耀如.2010.中国喀斯特.北京:高等教育出版社.
容丽,王世杰,卢红梅.2008.喀斯特峡谷区石漠化过程中土壤的生物地球化学特征.矿物岩石地球化学通报,27(s1):501-503.
王丁,姚健,杨雪,等.2011.干旱胁迫条件下6种喀斯特主要造林树种苗木叶片水势及吸水潜能变化.生态学报,31(8):2216-2226.
王天华,李玫,王沙生,等.1995.不同营养条件下杨槐混交对生长和杨树光合作用的影响.北京林业大学学报,17(4):62-66.
魏兴萍,刘文.2014.当前国际岩溶洞穴学界研究热点-袁道先院士访谈录.重庆师范大学学报:自然科学版,31(1):1-5.
吴沿友.2011.喀斯特适生植物固碳增汇策略.中国岩溶,30(4):461-465.
邢德科,吴沿友,吴沿胜,等.2016.麻疯树和枫杨幼苗对5种模拟喀斯特逆境的光合生理响应.中国岩溶,35(6):649-656.
姚永慧.2014.中国西南喀斯特石漠化研究进展与展望.地理科学进展,33(1):76-84.
郑元,赵忠,周慧,等.2010.刺槐树冠光合作用的空间异质性.生态学报,30(23):6399-6408.
朱东方.2010.刺槐种质资源评价及良种选育(硕士学位论文).泰安:山东农业大学.
Deng B,Du W,Liu C,et al.2012.Antioxidant response to drought,cold and nutrient stress in two ploidy levels of tobacco plants:Low resource requirement confers polytolerance in polyploids.Plant Growth Regulation,66:37-47.
Hang HT,Wu YY.2016.Quantification of photosynthetic inorganic carbon utilisation via a bidirectional stable carbon isotope tracer.Acta Geochimica,35:130-137.
Hu H.2010.Carbonic anhydrases are upstream regulators of CO2-controlled stomatal movements in guard cells.Nature Cell Biology,12:87-93.
Keenan TF,Hollinger DY,Bohrer G,et al.2013.Increase in forest water-use efficiency as atmospheric carbon dioxide concentrations rise.Nature,499:324-327.
Leuning R,Kelliher FM,Pury DGGD,et al.2010.Leaf nitrogen,photosynthesis,conductance and transpiration:Scaling from leaves to canopies.Plant,Cell&Environment,18:1183-1200.
Li H,Cheng Z.2015.Hoagland nutrient solution promotes the growth of cucumber seedlings under light-emitting diode light.Acta Agriculturae Scandinavica,65:74-82.
Li KR,Wang HH,Han G,et al.2008.Effects of brassinolide on the survival,growth and drought resistance of Robinia pseudoacacia seedlings under water-stress.New Forests,35:255-266.
Müller WEG,Qiang L,Schr9der HC,et al.2014.Carbonic anhydrase:A key regulatory and detoxifying enzyme for Karst plants.Planta,239:213-229.
Nie YP,Chen HS,Wang KL,et al.2014.Rooting characteristics of two widely distributed woody plant species growing in different karst habitats of southwest China.Plant Ecology,215:1099-1109.
Perez-Martin A,Michelazzo C,TorresRuiz JM,et al.2014.Regulation of photosynthesis and stomatal and mesophyll conductance under water stress and recovery in olive trees:Correlation with gene expression of carbonic anhydrase and aquaporins.Journal of Experimental Botany,65:3143-3156.
Sensua BM.2015.Spatial and short-temporal variability ofδ13Candδ15N and water-use efficiency in pine needles of the three forests along the most industrialized part of Poland.Water,Air&Soil Pollution,226:1-13.
Turan M,Güllüce M,akmak R,et al.2013.Effect of plant growth-promoting rhizobacteria strain on freezing injury and antioxidant enzyme activity of wheat and barley.Journal of Plant Nutrition,36:731-748.
Wang SJ,Liu QM,Zhang DF.2004.Karst rocky desertification in southwestern China:Geomorphology,landuse,impact and rehabilitation.Land Degradation&Development,15:115-121.
Wang Z,Wang M,Liu L,et al.2013.Physiological and proteomic responses of diploid and tetraploid black locust(Robinia pseudoacacia L.)subjected to salt stress.International Journal of Molecular Sciences,14:20299-20325.
Wang R,Wu YY,Xing DK,et al.2017.Biomass production of three biofuel energy plants’use of a new carbon resource by carbonic anhydrase in simulated karst soils:Mechanism and capacity.Energies,10:1370-1383.
Wu YY,Shi QQ,Wang K,et al.2011.An electrochemical approach coupled with Sb microelectrode to determine the activities of carbonic anhydrase in the plant leaves.Future Intelligent Information Systems,86:87-94.
Yu W,Liu Y,Song L,et al.2016.Effect of differential light quality on morphology,photosynthesis,and antioxidant enzyme activity in Camptotheca acuminata seedlings.Journal of Plant Growth Regulation,36:1-13.
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