铜绿假单胞菌检测能力验证结果与分析
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摘要
目的 分析实验室对饮用水中铜绿假单胞菌检测能力验证结果。方法 依据能力验证作业指导书幵参照GB4789.2-2016对样品进行稀释,根据GB8538-2016进行检测,同时对可疑菌落使用API20NE与VITEK2进行辅助鉴定。结果 样品18-N361、18-U838能力验证结果为满意。结论 通过此次能力验证,解决了饮用水样品的稀释问题,验证了API 20NE与VITEK2对可疑菌落进行辅助鉴定的准确性。VITEK2能缩短检验时间。
Objective To analyze the detection capability verification results of Pseudomonas aeruginosa in drinking water in laboratory. Methods The samples were tested according to GB 8538-2016, which were diluted according to the competency verification instruction and GB 4789.2-2016. The suspected colonies were identified by API 20 NE and VITEK2. Results The capability verification results of 18-N361 and 18-U838 were satisfactory.Conclusion Through this capability verification, the dilution problem of drinking water samples is solved, and the accuracy of API 20 NE and VITEK2 in assistant identification of suspected colonies is verified. VITEK2 can shorten the inspection time.
Objective To analyze the detection capability verification results of Pseudomonas aeruginosa in drinking water in laboratory. Methods The samples were tested according to GB 8538-2016, which were diluted according to the competency verification instruction and GB 4789.2-2016. The suspected colonies were identified by API 20 NE and VITEK2. Results The capability verification results of 18-N361 and 18-U838 were satisfactory.Conclusion Through this capability verification, the dilution problem of drinking water samples is solved, and the accuracy of API 20 NE and VITEK2 in assistant identification of suspected colonies is verified. VITEK2 can shorten the inspection time.
引文
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[3]王莉,李明,江志红,等.543株铜绿假单胞菌的临床分布及耐药性[J].解放军预防医学杂志,2017,(4):355-356,365.Wang L,Li M,Jiang ZH,et al.Clinical distribution and drug resistance of543 strains of Pseudomonas aeruginosa[J].J Prev Med Chin People's Liber Army,2017,(4):355-356,365.
[4]段江丼,胡汝源,杨红菊.大理州市售食品细菌性污染情冴分析[J].中国卫生检验杂志,2017,28(7):890-892.Duan JL,Hu RY,Yang HJ.Bacterial contamination analysis of food sold in Dali[J].Chin J Health Inspect,2017,28(7):890-892.
[5]周臣清,张娟,黄宝莹,等.广州市售瓶(桶)装水中铜绿假单胞菌假单胞菌污染现状及其耐药现象研究[J].中国酿造,2017,36(12):168-171.Zhou CQ,Zhang J,Huang BY,et al.Study on the contamination status and drug resistance of Pseudomonas aeruginosa in bottled(barreled)water in Guangzhou[J].Brew China,2017,36(12):168-171.
[6]刘思超,徐励琴,罗泽燕,等.223份桶装天然矿泉水和包装饮用水中铜绿假单胞菌假单胞菌的检测结果分析[J].预防医学情报杂志,2016,32(10):1071-1075.Liu SC,Xu LQ,Luo ZY,et al.Analysis of the detection results of Pseudomonas aeruginosa in 223 barreled natural mineral water and packaged drinking water[J].J Prev Med Inform,2016,32(10):1071-1075.
[7]郭辽朴,仁蕴慧,牛世文.2013-2014年漯河市桶装饮用水中铜绿假单胞菌假单胞菌污染状冴调查[J].中国卫生工程学,2017,(1):33-35.Guo LP,Ren YH,Niu SW.Investigation on the contamination of Pseudomonas aeruginosa in barreled drinking water in Luohe city from2013 to 2014[J].China Health Eng,2017,(1):33-35.
[8]苗杨.2014年-2015年沈阳市康平县桶装饮用水中铜绿假单胞菌假单胞菌的污染监测分析[J].航空航天医学杂志,2016,27(10):1286-1288.Miao Y.Surveillance and analysis of Pseudomonas aeruginosa contamination in barreled drinking water in Kangping county[J].J Aerosp Med,2016,27(10):1286-1288.
[9]曾晓琮,汪廷彩,周露,等.2015年广东省桶装饮用水中铜绿假单胞菌的污染调查和药敏性分析[J].食品安全质量检测学报,2018,(12):2965-2969.Zeng XZ,Wang TC,Zhou L,et al.Investigation and drug sensitivity analysis of Pseudomonas aeruginosa in barreled drinking water of Guangdong province in 2015[J].J Food Saf Qual,2018,(12):2965-2969.
[10]CNAS-RL02:2018能力验证觃则[S].CNAS-RL02:2018 Capability verification rules[S].
[11]GB 8538-2016食品安全国家标准饮用天然矿泉水检验斱法[S].GB 8538-2016 National food safety standard-Drinking natural mineral water inspection method[S].
[12]GB 4789.2-2016食品安全国家标准食品微生物学检验菌落总数测定[S].GB 4789.2-2016 National food safety standards-Food microbiology testing-Total number of colonies[S].
[13]ASAC-PT551(2018)矿泉水中铜绿假单胞菌的检测能力验证参试指导书[Z].ASAC-PT551(2018)Reference guidelines for verification of detection capability of pseudomonas aeruginosa in mineral water[Z].
[14]王立平,蔡雪凤.微生物实验室参加FEPAS能力验证计划的质量控制及对策[J].食品科技,2011,(7):256-260.Wang LP,Cai XF.Quality control and countermeasure of microbiology laboratory participating in FEPAS capability verification program[J].Food Sci Technol,2011,(7):256-260.
[15]甄珍.微生物能力验证实验结果分析[J].黑龙江农业科学,2011,(6):202-203.Zhen Z.Analysis of the results of microbial proficiency test[J].Heilongjiang Agric Sci,2011,(6):202-203.
[16]吉彦莉,郭勇峰,王敬辉,等.食品微生物学检测能力验证分析[J].公共卫生与预防医学,2015,(3):110-112.Ji YL,Guo YF,Wang JH,et al.Validation and analysis of food microbiology detection ability[J].Public Health Prev Med,2015,(3):110-112.
[17]贾东,李宏,王金玱,等.食品微生物学能力验证过程质量控制的研究[J].现代测量与实验室管理,2012,(6):49-52.Jia D,Li H,Wang JL,et al.Quality control of food microbiology proficiency verification process[J].Mod Meas Lab Manag,2012,(6):49-52
[18]蔡标,戴陈伟,吴纳新,等.沙门氏菌的分离与鉴定能力验证[J].食品安全质量检测学报,2018,(21):134-138.Cai B,Dai CW,Wu NX,et al.Isolation and identification of Salmonella[J].J Food Saf Qual,2018,(21):134-138.
[19]劳嘉倩.浅谈食品检测实验室质量控制技术[J].广东化工,2018,(21):56-57.Lao JQ.Talking about the quality control technology of food testing laboratory[J].Guangdong Chem Ind,2018,(21):56-57.
[20]汪桃花,代真真,王新文,等.能力验证对提升实验室技术水平的影响[J].现代食品,2018,(16):19-22.Wang TH,Dai ZZ,Wang XW,et al.The effect of proficiency testing on improving laboratory technology[J].Mod Food,2018,(16):19-22.
[2]魏光,叶英,沈继录.2009~2012年铜绿假单胞菌耐药性分析[J].中华医院感染学杂志,2013,(24):6115-6117.Wei G,Ye Y,Shen JL.Resistance analysis of Pseudomonas aeruginosa from 2009 to 2012[J].Chin J Hosp Infect,2013,(24):6115-6117.
[3]王莉,李明,江志红,等.543株铜绿假单胞菌的临床分布及耐药性[J].解放军预防医学杂志,2017,(4):355-356,365.Wang L,Li M,Jiang ZH,et al.Clinical distribution and drug resistance of543 strains of Pseudomonas aeruginosa[J].J Prev Med Chin People's Liber Army,2017,(4):355-356,365.
[4]段江丼,胡汝源,杨红菊.大理州市售食品细菌性污染情冴分析[J].中国卫生检验杂志,2017,28(7):890-892.Duan JL,Hu RY,Yang HJ.Bacterial contamination analysis of food sold in Dali[J].Chin J Health Inspect,2017,28(7):890-892.
[5]周臣清,张娟,黄宝莹,等.广州市售瓶(桶)装水中铜绿假单胞菌假单胞菌污染现状及其耐药现象研究[J].中国酿造,2017,36(12):168-171.Zhou CQ,Zhang J,Huang BY,et al.Study on the contamination status and drug resistance of Pseudomonas aeruginosa in bottled(barreled)water in Guangzhou[J].Brew China,2017,36(12):168-171.
[6]刘思超,徐励琴,罗泽燕,等.223份桶装天然矿泉水和包装饮用水中铜绿假单胞菌假单胞菌的检测结果分析[J].预防医学情报杂志,2016,32(10):1071-1075.Liu SC,Xu LQ,Luo ZY,et al.Analysis of the detection results of Pseudomonas aeruginosa in 223 barreled natural mineral water and packaged drinking water[J].J Prev Med Inform,2016,32(10):1071-1075.
[7]郭辽朴,仁蕴慧,牛世文.2013-2014年漯河市桶装饮用水中铜绿假单胞菌假单胞菌污染状冴调查[J].中国卫生工程学,2017,(1):33-35.Guo LP,Ren YH,Niu SW.Investigation on the contamination of Pseudomonas aeruginosa in barreled drinking water in Luohe city from2013 to 2014[J].China Health Eng,2017,(1):33-35.
[8]苗杨.2014年-2015年沈阳市康平县桶装饮用水中铜绿假单胞菌假单胞菌的污染监测分析[J].航空航天医学杂志,2016,27(10):1286-1288.Miao Y.Surveillance and analysis of Pseudomonas aeruginosa contamination in barreled drinking water in Kangping county[J].J Aerosp Med,2016,27(10):1286-1288.
[9]曾晓琮,汪廷彩,周露,等.2015年广东省桶装饮用水中铜绿假单胞菌的污染调查和药敏性分析[J].食品安全质量检测学报,2018,(12):2965-2969.Zeng XZ,Wang TC,Zhou L,et al.Investigation and drug sensitivity analysis of Pseudomonas aeruginosa in barreled drinking water of Guangdong province in 2015[J].J Food Saf Qual,2018,(12):2965-2969.
[10]CNAS-RL02:2018能力验证觃则[S].CNAS-RL02:2018 Capability verification rules[S].
[11]GB 8538-2016食品安全国家标准饮用天然矿泉水检验斱法[S].GB 8538-2016 National food safety standard-Drinking natural mineral water inspection method[S].
[12]GB 4789.2-2016食品安全国家标准食品微生物学检验菌落总数测定[S].GB 4789.2-2016 National food safety standards-Food microbiology testing-Total number of colonies[S].
[13]ASAC-PT551(2018)矿泉水中铜绿假单胞菌的检测能力验证参试指导书[Z].ASAC-PT551(2018)Reference guidelines for verification of detection capability of pseudomonas aeruginosa in mineral water[Z].
[14]王立平,蔡雪凤.微生物实验室参加FEPAS能力验证计划的质量控制及对策[J].食品科技,2011,(7):256-260.Wang LP,Cai XF.Quality control and countermeasure of microbiology laboratory participating in FEPAS capability verification program[J].Food Sci Technol,2011,(7):256-260.
[15]甄珍.微生物能力验证实验结果分析[J].黑龙江农业科学,2011,(6):202-203.Zhen Z.Analysis of the results of microbial proficiency test[J].Heilongjiang Agric Sci,2011,(6):202-203.
[16]吉彦莉,郭勇峰,王敬辉,等.食品微生物学检测能力验证分析[J].公共卫生与预防医学,2015,(3):110-112.Ji YL,Guo YF,Wang JH,et al.Validation and analysis of food microbiology detection ability[J].Public Health Prev Med,2015,(3):110-112.
[17]贾东,李宏,王金玱,等.食品微生物学能力验证过程质量控制的研究[J].现代测量与实验室管理,2012,(6):49-52.Jia D,Li H,Wang JL,et al.Quality control of food microbiology proficiency verification process[J].Mod Meas Lab Manag,2012,(6):49-52
[18]蔡标,戴陈伟,吴纳新,等.沙门氏菌的分离与鉴定能力验证[J].食品安全质量检测学报,2018,(21):134-138.Cai B,Dai CW,Wu NX,et al.Isolation and identification of Salmonella[J].J Food Saf Qual,2018,(21):134-138.
[19]劳嘉倩.浅谈食品检测实验室质量控制技术[J].广东化工,2018,(21):56-57.Lao JQ.Talking about the quality control technology of food testing laboratory[J].Guangdong Chem Ind,2018,(21):56-57.
[20]汪桃花,代真真,王新文,等.能力验证对提升实验室技术水平的影响[J].现代食品,2018,(16):19-22.Wang TH,Dai ZZ,Wang XW,et al.The effect of proficiency testing on improving laboratory technology[J].Mod Food,2018,(16):19-22.
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