SSR分子标记的研究进展
|
摘要
介绍SSR分子标记的开发方法,综述了SSR标记在通用性、遗传多样性与种质鉴定、遗传连锁图谱构建、基因定位与克隆、数量性状基因位点(QTLs)分析、DNA指纹图谱构建等研究中的应用及最新进展,对提高SSR分子标记电泳检测效率提出一些建议.同时展望了SSR分子标记的应用前景.
This paper introduced the development methods of SSR molecular markers, and summarized its application and recent new advances in the versatility, genetic diversity and germplasm identification, genetic linkage map construction, gene mapping and cloning, quantitative trait loci(QTLs) analysis and DNA fingerprinting construction. It also provided some suggestions for improving the efficiency of SSR molecular marker electrophoresis detection, and expected the application prospect of SSR molecular markers.
This paper introduced the development methods of SSR molecular markers, and summarized its application and recent new advances in the versatility, genetic diversity and germplasm identification, genetic linkage map construction, gene mapping and cloning, quantitative trait loci(QTLs) analysis and DNA fingerprinting construction. It also provided some suggestions for improving the efficiency of SSR molecular marker electrophoresis detection, and expected the application prospect of SSR molecular markers.
引文
[1] DING S,WANG S,HE K,et al.Large-scale analysis reveals that the genome features of simple sequence repeats are generally conserved at the family level in insects[J].BMC Genomics,2017,18(1):848.
[2] KAUR S,PANESAR P S,BERA M B,et al.Simple sequence repeat markers in genetic divergence and marker-assisted selection of rice cultivars:a review[J].Critical Reviews in Food Science & Nutrition,2015,55(1):41-49.
[3] KIM K S,SAPPINGTON T W.Microsatellite data analysis for population genetics[J].Methods Mol Biol,2013,1006:271-295.
[4] CHAMBERS G K,CURTIS C,MILLAR C D,et al.DNA fingerprinting in zoology:past,present,future[J].Investigative Genetics,2014,5(1):3.
[5] RASSMANN K,SCHL?TTERER C,TAUTZ D.Isolation of simple-sequence loci for use in polymerase chain reaction-based DNA fingerprinting[J].Electrophoresis,1991,12(2/3):113-118.
[6] KRESOVICH S,SZEWC-MCFADDEN A K,BLIEK S M,et al.Abundance and characterization of simple-sequence repeats (SSRs) isolated from a size-fractionated genomic library of Brassica napus,L.(rapeseed)[J].Theoretical and Applied Genetics,1995,91(2):206-211.
[7] SZEWC-MCFADDEN A K,KRESOVICH S,BLIEK S M,et al.Identification of polymorphic,conserved simple sequence repeats (SSRs) in cultivated Brassica species[J].Theoretical & Applied Genetics,1996,93(4):534-538.
[8] UJINO T,KAWAHARA T,TSUMURA Y,et al.Development and polymorphism of simple sequence repeat DNA markers for Shorea curtisii,and other Dipterocarpaceae species[J].Heredity,1999,99 (1/2):11-15.
[9] 敖日格乐,贾晓,葛台明.SSR分子标记的开发策略概述[J].湖北民族学院学报(自然科学版),2009,27(4):462-467.
[10] 孔杰,蒋晓红,周洲,等.磁珠富集法开发长臀鮠微卫星分子标记[J].贵州农业科学,2016,44(7):14-17.
[11] ZERBO G C,KONRAD H,OUEDRAOGO M,et al.Fourteen simple-sequence repeats newly developed for population genetic studies in Prosopis africana (Fabaceae-Mimosoideae)[J].Bmc Research Notes,2017,10(1):437.
[12] TANAKA K,OHTAKE R,YOSHIDA S,et al.Effective DNA fragmentation technique for simple sequence repeat detection with a microsatellite-enriched library and high-throughput sequencing[J].Biotechniques,2017,62(4):180-182.
[13] CIFARELLI R A,GALLITELLI M,CELLINI F.Random amplified hybridization microsatellites (RAHM):isolation of a new class of microsatellite-containing DNA clones[J].Nucleic Acids Research,1995,23(18):3802-3805.
[14] FISHER P J,GARDNER R C,RICHARDSON T E.Single locus microsatellites isolated using 5′ anchored PCR[J].Nucleic Acids Research,1996,24(21):4369-4371.
[15] 李明芳,郑学勤.荔枝SSR标记的研究[J].遗传,2004,26(6):911-916.
[16] 艾呈祥,余贤美,张力思,等.甜樱桃SSR标记的选择性扩增微卫星(SAM)法筛选(英文)[J].园艺学报,2007,34(2):311-316.
[17] 程小毛,黄晓霞.SSR标记开发及其在植物中的应用[J].中国农学通报,2011,27(5):304-307.
[18] 罗兵,孙海燕,徐港明,等.SSR分子标记研究进展[J].安徽农业科学,2013(12):5210-5212.
[19] KANTETY R V,ROTA M L,MATTHEWS D E,et al.Data mining for simple sequence repeats in expressed sequence tags from barley,maize,rice,sorghum and wheat[J].Plant Molecular Biology,2002,48(5/6):501-510.
[20] 刘列钊,林呐.油菜简单重复序列SSR(simple sequence repeat)研究进展[J].生命科学,2004,16(3):173-176.
[21] KIRUNGU J N,DENG Y,CAI X,et al.Simple sequence repeat (SSR) genetic linkage map of D genome diploid cotton derived from an interspecific cross between gossypium davidsonii and gossypium klotzschianum[J].International Journal of Molecular Sciences,2018,19(1):204.
[22] FRASER L G ,HARVEY C F ,CROWHURST R N ,et al.EST-derived microsatellites from Actinidiaspecies and their potential for mapping[J].Theoretical and Applied Genetics,2004,108(6):1010-1016.
[23] SAHA M C,MIAN M A R,EUJAYL I,et al.Tall fescue EST-SSR markers with transferability across several grass species[J].Theoretical & Applied Genetics,2004,109(4):783-791.
[24] ZALAPA J E,SIMON P.Using next-generation sequencing approaches to isolate simple sequence repeat (SSR) loci in the plant science[J].American Journal of Botany,2012,99(2):193-208.
[25] 闫蕊,阮成江,杜维,等.RNA-seq技术开发油茶目的基因SSR标记[J].分子植物育种,2018,16(8):142-150.
[26] BHARTI R,KUMAR S,PAREKH M J.Development of genomic simple sequence repeat (gSSR) markers in cumin and their application in diversity analyses and cross-transferability[J].Industrial Crops and Products,2018,111:158-164.
[27] ABUZAYED M A,GOKTAY M,ALLMER J,et al.Development of genomic simple sequence repeat markers in faba bean by next-generation sequencing[J].Plant Molecular Biology Reporter,2017,35(1):61-71.
[28] DECROOCQ V,FAVé M G,HAGEN L,et al.Development and transferability of apricot and grape EST microsatellite markers across taxa[J].Theoretical & Applied Genetics,2003,106(5):912-922.
[29] 陈仕勇,马啸,张新全,等.不同来源SSR和EST-SSR在披碱草属和鹅观草属物种中的通用性分析[J].草业学报,2016,25(2):132-140.
[30] PAGAR T A,AKHARE A A,GAHUKAR S J,et al.DNA fingerprinting of soybean (Glycine max l.) Genotypes by using simple sequence repeats (SSR) markers[J].International Journal of Chemical Studies,2017,5(5):674-679.
[31] 卢玉飞,蒋建雄,易自力.玉米SSR引物和甘蔗EST-SSR引物在芒属中的通用性研究[J].草业学报,2012,21(5):86-95.
[32] MUHAMMAD R W,QAYYUM A,AHMAD M Q,et al.Characterization of maize genotypes for genetic diversity on the basis of inter simple sequence repeats[J].Genetics & Molecular Research,2017,16(1).doi:10.4238/gmr16019438.
[33] 郭林林.丹参基因组SSR标记的开发及其在连锁图谱构建的应用[D].泰安:山东农业大学,2016.
[34] 熊立仲,王石平,刘克德,等.微卫星DNA和AFLP标记在水稻分子标记连锁图上的分布[J].植物生态学报(英文版),1998(7):605-614.
[35] 高美玲,朱子成,高鹏,等.甜瓜重组自交系群体SSR遗传图谱构建及纯雌性基因定位[J].园艺学报,2011,38(7):1308-1316.
[36] 向道权,曹海河,曹永国,等.玉米SSR遗传图谱的构建及产量性状基因定位[J].遗传学报,2001,28(8):778-784.
[37] 王凤格,刘贤德,王振华,等.玉米抗甘蔗花叶病毒QTL的初步研究[J].作物学报,2003,29(1):69-74.
[38] 杜虎.分子标记辅助选择转移抗稻瘟病和白叶枯病基因改良水稻恢复系的研究[D].南昌:江西农业大学,2012.
[39] 梁凤山,邓启云,王岳光,等.应用SSR方法对野生稻高产基因的分子标记辅助选择[C]//全国作物遗传育种学术研讨会,合肥,2003.
[40] 尹玲,张晨,向江,等.我国新育成葡萄品种SSR指纹图谱的建立[J].果树学报,2015,32(3):366-373.
[41] 范建光.基于SSR标记的西瓜DNA指纹图谱库的构建[D].泰安:山东农业大学,2013.
[42] 李新海,焦少杰.两种凝胶电泳系统对SSR标记多态性的影响[J].华北农学报,2001,16(2):43-48.
[43] 穆建新,李殿荣,郭蔼光,等.用SSR分子标记检测杂交油菜秦优7号的种子纯度[J].西北农业学报,2010,19(11):64-68.
[44] 汤天泽,肖小余,税红霞.SSR标记与形态学方法鉴定杂交油菜纯度的比较研究[J].分子植物育种,2008,6(2):377-380.
[45] 梅德圣,李云昌,胡琼,等.甘蓝型油菜中油杂8号种子纯度的SSR鉴定[J].中国农学通报,2006,22(5):49-52.
[46] 宋贤勇,包月红,杨俊涛,等.利用SSR标记鉴定甘蓝型油菜秦优11号种子纯度[J].分子植物育种,2010,8(3):497-500.
[47] 胡依依,隋正红,彭冲,等.聚丙烯酰胺凝胶与毛细管电泳检测龙须菜SSR位点的比较研究[J].中国海洋大学学报(自然科学版),2018(3):65-72.
[48] 李新海,焦少杰.两种凝胶电泳系统对SSR标记多态性的影响[J].华北农学报,2001,16(2):43-48.
[49] CHEN Y Q,LI F X,LI X K,et al.Fluorescent detection and capillary electrophoresis in tobacco SSR[J].Chinese Tobacco Science,2011,32(2):66-70.
[50] MEI Y I,WANG F G,ZHAO J R,et al.Comparison of two maize SSR detection methods:capillary electrophoresis with fluorescence detection method and denaturing PAGE silver-staining detection method[J].Acta Agriculturae Boreali-Sinica,2006,21(5):64-67.
[51] HAO C,WANG L,JIA J,et al.Comparison of fluorescence and silver--staining detection systems of microsatellite markers[J].Acta Agronomica Sinica,2005,31(2):144-149.
[52] 李学军,曹丽华,王辉,等.半子粒小麦高分子量麦谷蛋白亚基的SDS-PAGE分析方法[J].河南农业大学学报,2003,37(3):209-212.
[53] 胡建斌,李静,袁鸣,等.PAGE凝胶制备方法和银染方法的改进[J].江西农业大学学报,2009,31(4):742-745.
[54] 梁宏伟,王长忠,李忠,等.聚丙烯酰胺凝胶快速、高效银染方法的建立[J].遗传,2008,30(10):1379-1382.
[55] 张艺萍,罗文富,杨艳丽.马铃薯和番茄晚疫病菌全基因组DNA的RAPD多态性分析[J].云南农业大学学报,2007,22(3):352-357.
[56] GHIMIRE S R,HYDE K D,HODGKISS I J,et al.Phenotypes of Phytophthora infestans in Nepal:mating types and metalaxyl sensitivity[J].Potato Research,2001,44(4):337-347.
[57] 刘蕊,钟进良,饶小珍,等.番茄和马铃薯晚疫病菌的SSR基因型分析[J].广东农业科学,2010,37(10):149-151.
[58] 李立群,王培,王小利,等.SSR标记变性聚丙烯酰胺凝胶电泳方法改进[J].安徽农业科学,2012,40(34):16541-16544.
[59] 韩永亮,常金华.聚丙烯酰胺凝胶电泳的两种染色方法对SSR标记的影响[J].园艺与种苗,2006,26(3):176-177.
[60] 张芳,权军利,单卫星.聚丙烯酰胺凝胶电泳检测致病疫霉菌SSR标记方法改良[J].安徽农业科学,2011,39(27):3-5.
[61] 郭培国,刘文杰,李海洋,等.一种快速有效检测SSR标记的非变性聚丙烯酰胺凝胶的银染方法[J].广州大学学报(自然科学版),2016,15(4):8-12.
[62] LI L,FANG Z,ZHOU J,et al.An accurate and efficient method for large-scale SSR genotyping and applications[J].Nucleic Acids Research,2017,45(10):e88.doi:10.1093/nar/gkx093.
[2] KAUR S,PANESAR P S,BERA M B,et al.Simple sequence repeat markers in genetic divergence and marker-assisted selection of rice cultivars:a review[J].Critical Reviews in Food Science & Nutrition,2015,55(1):41-49.
[3] KIM K S,SAPPINGTON T W.Microsatellite data analysis for population genetics[J].Methods Mol Biol,2013,1006:271-295.
[4] CHAMBERS G K,CURTIS C,MILLAR C D,et al.DNA fingerprinting in zoology:past,present,future[J].Investigative Genetics,2014,5(1):3.
[5] RASSMANN K,SCHL?TTERER C,TAUTZ D.Isolation of simple-sequence loci for use in polymerase chain reaction-based DNA fingerprinting[J].Electrophoresis,1991,12(2/3):113-118.
[6] KRESOVICH S,SZEWC-MCFADDEN A K,BLIEK S M,et al.Abundance and characterization of simple-sequence repeats (SSRs) isolated from a size-fractionated genomic library of Brassica napus,L.(rapeseed)[J].Theoretical and Applied Genetics,1995,91(2):206-211.
[7] SZEWC-MCFADDEN A K,KRESOVICH S,BLIEK S M,et al.Identification of polymorphic,conserved simple sequence repeats (SSRs) in cultivated Brassica species[J].Theoretical & Applied Genetics,1996,93(4):534-538.
[8] UJINO T,KAWAHARA T,TSUMURA Y,et al.Development and polymorphism of simple sequence repeat DNA markers for Shorea curtisii,and other Dipterocarpaceae species[J].Heredity,1999,99 (1/2):11-15.
[9] 敖日格乐,贾晓,葛台明.SSR分子标记的开发策略概述[J].湖北民族学院学报(自然科学版),2009,27(4):462-467.
[10] 孔杰,蒋晓红,周洲,等.磁珠富集法开发长臀鮠微卫星分子标记[J].贵州农业科学,2016,44(7):14-17.
[11] ZERBO G C,KONRAD H,OUEDRAOGO M,et al.Fourteen simple-sequence repeats newly developed for population genetic studies in Prosopis africana (Fabaceae-Mimosoideae)[J].Bmc Research Notes,2017,10(1):437.
[12] TANAKA K,OHTAKE R,YOSHIDA S,et al.Effective DNA fragmentation technique for simple sequence repeat detection with a microsatellite-enriched library and high-throughput sequencing[J].Biotechniques,2017,62(4):180-182.
[13] CIFARELLI R A,GALLITELLI M,CELLINI F.Random amplified hybridization microsatellites (RAHM):isolation of a new class of microsatellite-containing DNA clones[J].Nucleic Acids Research,1995,23(18):3802-3805.
[14] FISHER P J,GARDNER R C,RICHARDSON T E.Single locus microsatellites isolated using 5′ anchored PCR[J].Nucleic Acids Research,1996,24(21):4369-4371.
[15] 李明芳,郑学勤.荔枝SSR标记的研究[J].遗传,2004,26(6):911-916.
[16] 艾呈祥,余贤美,张力思,等.甜樱桃SSR标记的选择性扩增微卫星(SAM)法筛选(英文)[J].园艺学报,2007,34(2):311-316.
[17] 程小毛,黄晓霞.SSR标记开发及其在植物中的应用[J].中国农学通报,2011,27(5):304-307.
[18] 罗兵,孙海燕,徐港明,等.SSR分子标记研究进展[J].安徽农业科学,2013(12):5210-5212.
[19] KANTETY R V,ROTA M L,MATTHEWS D E,et al.Data mining for simple sequence repeats in expressed sequence tags from barley,maize,rice,sorghum and wheat[J].Plant Molecular Biology,2002,48(5/6):501-510.
[20] 刘列钊,林呐.油菜简单重复序列SSR(simple sequence repeat)研究进展[J].生命科学,2004,16(3):173-176.
[21] KIRUNGU J N,DENG Y,CAI X,et al.Simple sequence repeat (SSR) genetic linkage map of D genome diploid cotton derived from an interspecific cross between gossypium davidsonii and gossypium klotzschianum[J].International Journal of Molecular Sciences,2018,19(1):204.
[22] FRASER L G ,HARVEY C F ,CROWHURST R N ,et al.EST-derived microsatellites from Actinidiaspecies and their potential for mapping[J].Theoretical and Applied Genetics,2004,108(6):1010-1016.
[23] SAHA M C,MIAN M A R,EUJAYL I,et al.Tall fescue EST-SSR markers with transferability across several grass species[J].Theoretical & Applied Genetics,2004,109(4):783-791.
[24] ZALAPA J E,SIMON P.Using next-generation sequencing approaches to isolate simple sequence repeat (SSR) loci in the plant science[J].American Journal of Botany,2012,99(2):193-208.
[25] 闫蕊,阮成江,杜维,等.RNA-seq技术开发油茶目的基因SSR标记[J].分子植物育种,2018,16(8):142-150.
[26] BHARTI R,KUMAR S,PAREKH M J.Development of genomic simple sequence repeat (gSSR) markers in cumin and their application in diversity analyses and cross-transferability[J].Industrial Crops and Products,2018,111:158-164.
[27] ABUZAYED M A,GOKTAY M,ALLMER J,et al.Development of genomic simple sequence repeat markers in faba bean by next-generation sequencing[J].Plant Molecular Biology Reporter,2017,35(1):61-71.
[28] DECROOCQ V,FAVé M G,HAGEN L,et al.Development and transferability of apricot and grape EST microsatellite markers across taxa[J].Theoretical & Applied Genetics,2003,106(5):912-922.
[29] 陈仕勇,马啸,张新全,等.不同来源SSR和EST-SSR在披碱草属和鹅观草属物种中的通用性分析[J].草业学报,2016,25(2):132-140.
[30] PAGAR T A,AKHARE A A,GAHUKAR S J,et al.DNA fingerprinting of soybean (Glycine max l.) Genotypes by using simple sequence repeats (SSR) markers[J].International Journal of Chemical Studies,2017,5(5):674-679.
[31] 卢玉飞,蒋建雄,易自力.玉米SSR引物和甘蔗EST-SSR引物在芒属中的通用性研究[J].草业学报,2012,21(5):86-95.
[32] MUHAMMAD R W,QAYYUM A,AHMAD M Q,et al.Characterization of maize genotypes for genetic diversity on the basis of inter simple sequence repeats[J].Genetics & Molecular Research,2017,16(1).doi:10.4238/gmr16019438.
[33] 郭林林.丹参基因组SSR标记的开发及其在连锁图谱构建的应用[D].泰安:山东农业大学,2016.
[34] 熊立仲,王石平,刘克德,等.微卫星DNA和AFLP标记在水稻分子标记连锁图上的分布[J].植物生态学报(英文版),1998(7):605-614.
[35] 高美玲,朱子成,高鹏,等.甜瓜重组自交系群体SSR遗传图谱构建及纯雌性基因定位[J].园艺学报,2011,38(7):1308-1316.
[36] 向道权,曹海河,曹永国,等.玉米SSR遗传图谱的构建及产量性状基因定位[J].遗传学报,2001,28(8):778-784.
[37] 王凤格,刘贤德,王振华,等.玉米抗甘蔗花叶病毒QTL的初步研究[J].作物学报,2003,29(1):69-74.
[38] 杜虎.分子标记辅助选择转移抗稻瘟病和白叶枯病基因改良水稻恢复系的研究[D].南昌:江西农业大学,2012.
[39] 梁凤山,邓启云,王岳光,等.应用SSR方法对野生稻高产基因的分子标记辅助选择[C]//全国作物遗传育种学术研讨会,合肥,2003.
[40] 尹玲,张晨,向江,等.我国新育成葡萄品种SSR指纹图谱的建立[J].果树学报,2015,32(3):366-373.
[41] 范建光.基于SSR标记的西瓜DNA指纹图谱库的构建[D].泰安:山东农业大学,2013.
[42] 李新海,焦少杰.两种凝胶电泳系统对SSR标记多态性的影响[J].华北农学报,2001,16(2):43-48.
[43] 穆建新,李殿荣,郭蔼光,等.用SSR分子标记检测杂交油菜秦优7号的种子纯度[J].西北农业学报,2010,19(11):64-68.
[44] 汤天泽,肖小余,税红霞.SSR标记与形态学方法鉴定杂交油菜纯度的比较研究[J].分子植物育种,2008,6(2):377-380.
[45] 梅德圣,李云昌,胡琼,等.甘蓝型油菜中油杂8号种子纯度的SSR鉴定[J].中国农学通报,2006,22(5):49-52.
[46] 宋贤勇,包月红,杨俊涛,等.利用SSR标记鉴定甘蓝型油菜秦优11号种子纯度[J].分子植物育种,2010,8(3):497-500.
[47] 胡依依,隋正红,彭冲,等.聚丙烯酰胺凝胶与毛细管电泳检测龙须菜SSR位点的比较研究[J].中国海洋大学学报(自然科学版),2018(3):65-72.
[48] 李新海,焦少杰.两种凝胶电泳系统对SSR标记多态性的影响[J].华北农学报,2001,16(2):43-48.
[49] CHEN Y Q,LI F X,LI X K,et al.Fluorescent detection and capillary electrophoresis in tobacco SSR[J].Chinese Tobacco Science,2011,32(2):66-70.
[50] MEI Y I,WANG F G,ZHAO J R,et al.Comparison of two maize SSR detection methods:capillary electrophoresis with fluorescence detection method and denaturing PAGE silver-staining detection method[J].Acta Agriculturae Boreali-Sinica,2006,21(5):64-67.
[51] HAO C,WANG L,JIA J,et al.Comparison of fluorescence and silver--staining detection systems of microsatellite markers[J].Acta Agronomica Sinica,2005,31(2):144-149.
[52] 李学军,曹丽华,王辉,等.半子粒小麦高分子量麦谷蛋白亚基的SDS-PAGE分析方法[J].河南农业大学学报,2003,37(3):209-212.
[53] 胡建斌,李静,袁鸣,等.PAGE凝胶制备方法和银染方法的改进[J].江西农业大学学报,2009,31(4):742-745.
[54] 梁宏伟,王长忠,李忠,等.聚丙烯酰胺凝胶快速、高效银染方法的建立[J].遗传,2008,30(10):1379-1382.
[55] 张艺萍,罗文富,杨艳丽.马铃薯和番茄晚疫病菌全基因组DNA的RAPD多态性分析[J].云南农业大学学报,2007,22(3):352-357.
[56] GHIMIRE S R,HYDE K D,HODGKISS I J,et al.Phenotypes of Phytophthora infestans in Nepal:mating types and metalaxyl sensitivity[J].Potato Research,2001,44(4):337-347.
[57] 刘蕊,钟进良,饶小珍,等.番茄和马铃薯晚疫病菌的SSR基因型分析[J].广东农业科学,2010,37(10):149-151.
[58] 李立群,王培,王小利,等.SSR标记变性聚丙烯酰胺凝胶电泳方法改进[J].安徽农业科学,2012,40(34):16541-16544.
[59] 韩永亮,常金华.聚丙烯酰胺凝胶电泳的两种染色方法对SSR标记的影响[J].园艺与种苗,2006,26(3):176-177.
[60] 张芳,权军利,单卫星.聚丙烯酰胺凝胶电泳检测致病疫霉菌SSR标记方法改良[J].安徽农业科学,2011,39(27):3-5.
[61] 郭培国,刘文杰,李海洋,等.一种快速有效检测SSR标记的非变性聚丙烯酰胺凝胶的银染方法[J].广州大学学报(自然科学版),2016,15(4):8-12.
[62] LI L,FANG Z,ZHOU J,et al.An accurate and efficient method for large-scale SSR genotyping and applications[J].Nucleic Acids Research,2017,45(10):e88.doi:10.1093/nar/gkx093.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |