流式细胞术研究细胞增殖的方法与技术
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摘要
流式细胞术是检测细胞增殖的一种重要手段,较以往的检测方法,流式细胞仪的优点在于能够实现多荧光指标同时检测,并且可以对混合样品中的特定细胞进行增殖分析,本文就目前常用细胞增殖检测染料的检测原理,应用范围及染色方法作进行阐述。
Flow cytometry is an important means to detect cell proliferation. Compared with previous detection methods, flow cytometry has the advantages of simultaneous detection of multiple fluorescence indicators, and can perform proliferation analysis on specific cells in mixed samples. At present, the detection principle, application range and dyeing method of cell proliferation detection dyes are commonly used.
Flow cytometry is an important means to detect cell proliferation. Compared with previous detection methods, flow cytometry has the advantages of simultaneous detection of multiple fluorescence indicators, and can perform proliferation analysis on specific cells in mixed samples. At present, the detection principle, application range and dyeing method of cell proliferation detection dyes are commonly used.
引文
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[20]Luzyanina T,Cupovic J,Ludewig B,et al.Mathematical models for CFSE labelled lymphocyte dynamics:asymmetry and time-lag in division[J].Journal of Mathematical Biology,2014,69(6-7):1547-1583.
[2]Dapena C,Bravo I,Cuadrado A,et al.Nuclear and Cell Morphological Changes during the Cell Cycle and Growth of the Toxic Dinoflagellate Alexandrium minutum[J].Protist,2015,66(1):146-160.
[3]Uzlikova M,Nohynkova E.The effect of metronidazole on the cell cycle and DNA in metronidazole-susceptible and resistant Giardia cell lines[J].Molecular&Biochemical Parasitology,2014,198(2):75-81.
[4]柏春玲,廖泽容,张巧,等.应用流式细胞术快速测定细胞周期的DNA一步法[J].昆明医科大学学报,2017,38(2):10-13.
[5]Carbonari M.New use for an old reagent:Cell cycle analysis of DNA content using flow cytometry in formamide treated cells[J].Cytometry,2016,89(5):498-503.
[6]户乃丽,徐晓雪,邹林樾,等.利用405nm激光激发Hoechst33342染色细胞DNA的流式细胞技术[J].现代生物医学进展,2015,15(33):6406-6409.
[7]Buzina AR,Pinto FE,Nieschke K,et al.Replacement of specific markers for apoptosis and necrosis by nuclear morphology for affordable cytometry[J].Journal of Immunological Methods,2015,420(3):24-30.
[8]申雪沂,郭佳倩,牛长敏,等.Stra8过表达精原细胞的细胞周期同步化方法的建立[J].中国男科杂志,2017,31(6):3-7.
[9]Medina-Reyes EI,Bucio-López L,Freyre-Fonseca V,et al.Cell cycle synchronization reveals greater G2/M-phase accumulation of lung epithelial cells exposed to titanium dioxide nanoparticles[J].Environ Sci Pollut Res,2015,22(9):3976-3982.
[10]Tong J,Sun D,Yang C,et al.Serum starvation and thymidine double blocking achieved efficient cell cycle synchronization and altered the expression of p27, p53, bcl-2 in canine breast cancer cells[J]. Research in Veterinary Science,2016(4):10-14.
[11]邹林樾,徐晓雪,户乃丽.鸡红细胞标准品在胶质瘤细胞DNA周期检测中的意义[J].继续医学教育,2015,29(10):152-153.
[12]Massey AJ.Multiparametric Cell Cycle Analysis Using the Operetta High-Content Imager and Harmony Software with PhenoLOGIC[J].PLOS ONE,2015,10(7):e0134306.
[13]Zhao P,Fu JL,Yao BY,et al.S phase cell percentage normalized BrdU incorporation rate,a new parameter for determining S arrest[J].Biomed Environ Sci,2014,27(3):215-219.
[14]Harris L,Zalucki O,Piper M.BrdU/EdU dual labeling to determine the cell-cycle dynamics of defined cellular subpopulations[J].Journal of Molecular Histology,2018,49(3):229-234.
[15]Pereira PD,Serra-Caetano A,Cabrita M,et al.Quantification of cell cycle kinetics by EdU(5-ethynyl-2'-deoxyuridine)-coupled-fluorescence-intensity analysis[J].Oncotarget,2017,8(25):40514-40532.
[16]Bocharov G,Luzyanina T,Cupovic J,et al.Asymmetry of cell division in CFSE-based lymphocyte proliferation analysis[J].Front Immunol,2013,4(9):264.
[17]Rodríguez-Pardo VM,Vernot JP.Mesenchymal stem cells promote a primitive phenotype CD34+c-kit in human cord blood-derived hematopoietic stem cells during ex vivo expansion[J].Cell Mol Biol Lett,2013,18(1):11-33.
[18]杜振兰,陈鹏,封志纯.比较不同浓度CFSE荧光标记脐血细胞后存活率的实验研究[J].中华临床医师杂志,2016,10(3):368-372.
[19]Lyons AB,Blake SJ,Doherty KV.Flow Cytometric Analysis of Cell Division by Dilution of CFSE and Related Dyes[J].Current Protocols in Cytometry,2013(9):64.
[20]Luzyanina T,Cupovic J,Ludewig B,et al.Mathematical models for CFSE labelled lymphocyte dynamics:asymmetry and time-lag in division[J].Journal of Mathematical Biology,2014,69(6-7):1547-1583.
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