连作植烟土壤酚酸类物质积累特征及其相互作用关系分析
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摘要
为探明连作植烟土壤酚酸类物质积累特征及其相互作用关系,利用高效液相色谱外标法检测了同一植烟区域不同种植年限土壤(4、6、8、14和16年)浸提液中酚酸类物质的种类和含量,并基于曲线估计方法和逐步回归模型,探讨了酚酸类物质在连作条件下的积累特征及其相互作用关系。结果表明,8种酚酸在不同连作植烟土壤中被检出,且其中6种具有明显的积累趋势;曲线估计分析表明不同酚酸积累特征存在差异,其中间苯三酚、阔马酸、对羟基苯甲酸、香草酸和香兰素以增长曲线趋势积累,阿魏酸含量以幂函数趋势积累。相关性分析和逐步回归分析表明,间苯三酚和阿魏酸含量之间存在互相促进关系,阿魏酸对阔马酸含量具有促进作用,香草酸对对羟基苯甲酸含量具有促进作用,香兰素和苯甲酸分别对香草酸含量存在促进和抑制作用;香草酸和苯甲酸对香兰素含量有促进作用。综上所述,连作导致植烟土壤酚酸类物质积累,且积累特征存在差异;酚酸类物质间存在不同程度促进或抑制作用,其作用方式取决于酚酸种类。
To identify the accumulation characteristics and interactions of phenolic acids in continuous cropping tobacco-planted soils,the types and contents of phenolic acids in the extracts of soil in different planted years(4,6,8,14 and 16 years)in the same tobacco planting area were collected and determined using high performance liquid chromatography(HPLC)external standard method,and the accumulation characteristics and interactions of phenolic acids under continuous cropping conditions were discussed based on the curve estimation method and stepwise regression model.Eight kinds of phenolic acids were detected in different continuous cropping tobacco-planted soils and six of them had obvious accumulation trend.Curve estimation analysis showed that there were differences in the accumulation characteristics of different phenolic acids,and among them the accumulative trend of the growth curve was found for phloroglucinol,coumalic acid,p-hydroxybenzoic acid,vanillic acid and vanillin,and the ferulic acid content accumulated as a power function.Correlation and stepwise regression analysis showed that there was a mutual promotion relationship between the content of phloroglucinol and ferulic acid,ferulic acid had a promoting effect on the content of lauric acid,and vanillic acid had a promoting effect on the content of p-hydroxybenzoic acid.Lanthanol and benzoic acid had effect of promoting and inhibiting vanillic acid content respectively,vanillic acid and benzoic acid had a promoting effect on vanillin content.Therefore,under the experimental conditions,continuous cropping resulted in the accumulation of phenolic acids in tobacco-planted soil and there were differences in their accumulation characteristics.Phenolic acids have different degrees of promotion or inhibition and their mode of action depends on the type of phenolic acids.
To identify the accumulation characteristics and interactions of phenolic acids in continuous cropping tobacco-planted soils,the types and contents of phenolic acids in the extracts of soil in different planted years(4,6,8,14 and 16 years)in the same tobacco planting area were collected and determined using high performance liquid chromatography(HPLC)external standard method,and the accumulation characteristics and interactions of phenolic acids under continuous cropping conditions were discussed based on the curve estimation method and stepwise regression model.Eight kinds of phenolic acids were detected in different continuous cropping tobacco-planted soils and six of them had obvious accumulation trend.Curve estimation analysis showed that there were differences in the accumulation characteristics of different phenolic acids,and among them the accumulative trend of the growth curve was found for phloroglucinol,coumalic acid,p-hydroxybenzoic acid,vanillic acid and vanillin,and the ferulic acid content accumulated as a power function.Correlation and stepwise regression analysis showed that there was a mutual promotion relationship between the content of phloroglucinol and ferulic acid,ferulic acid had a promoting effect on the content of lauric acid,and vanillic acid had a promoting effect on the content of p-hydroxybenzoic acid.Lanthanol and benzoic acid had effect of promoting and inhibiting vanillic acid content respectively,vanillic acid and benzoic acid had a promoting effect on vanillin content.Therefore,under the experimental conditions,continuous cropping resulted in the accumulation of phenolic acids in tobacco-planted soil and there were differences in their accumulation characteristics.Phenolic acids have different degrees of promotion or inhibition and their mode of action depends on the type of phenolic acids.
引文
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[12]Liu P,Liu Z H,Wang C B,et al.Effects of three longchain fatty acids present in peanut(Arachishypogaea L.)root exudates on its own growth and the soil enzymes activities[J].Allelopathy Journal,2012,29(1):13-24.
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[14]Qu X H,Wang J G.Effect of amendments with different phenolic acids on soil microbial biomass,activity,and community diversity[J].Applied Soil Ecology,2008,39(2):172-179.
[15]Lu X F,Zhang H,Lyu S S,et al.Effects of exogenous phenolic acids on photosystem functions and photosynthetic electron transport rate in strawberry leaves[J].Photosynthetica,2018,56(2):616-622.
[16]符建国,易建华,贾志红,等.轮作与连作烟田根际土壤酸性有机组分的初步分离与鉴定[J].中国烟草科学,2011,32(6):67-71.
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[18]陈冬梅,黄锦文,柯文辉,等.连作烟草根际土壤化感潜力评价及化感物质鉴定[J].中国烟草学报,2012,18(1):46-52.
[19]尤垂淮,高峰,王峰吉,等.连作对云南烤烟根际微生态及烟叶产质量的影响[J].中国烟草学报,2015,21(1):60-67.
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[22]徐婷,沈方科,石保峰,等.烟草根系分泌的酚酸及毒性[C]//广西烟草学会2013年学术年会,2013.
[23]张克勤,徐婷,沈方科,等.烟草根系分泌的酚酸及自毒效应[J].西南农业学报,2013,26(6):2552-2557.
[24]Greven S,Kneib T.Noncrossing quantile regression curve estimation[J].Biometrika,2010,97(4):825.
[25]Blum U.Allelopathic interactions involving phenolic acids[J].Journal of Nematology,1996,28(3):259-267.
[26]李亮亮,李天来,张恩平,等.四种酚酸物质在土壤中降解的研究[J].土壤通报,2010,(6):1460-1465.
[27]Song D.Who says rookie not data analysis(SPSS)(full color)(Chinese Edition)[M].Beijing:Publishing House of Electronic Industry,2016.
[28]李培栋,王兴祥,李奕林,等.连作花生土壤中酚酸类物质的检测及其对花生的化感作用[J].生态学报,2010,30(8):2128-2134.
[29]孙海兵,毛志泉,朱树华.环渤海湾地区连作苹果园土壤中酚酸类物质变化[J].生态学报,2011,31(1):90-97.
[30]李贺勤,刘奇志,张林林,等.草莓连作土壤酚酸类物质积累对土壤线虫的影响[J].生态学杂志,2014,33(1):169-175.
[31]谭秀梅,王华田,孔令刚,等.杨树人工林连作土壤中酚酸积累规律及对土壤微生物的影响[J].山东大学学报(理学版),2008,43(1):14-19.
[32]Li X G,Ding C F,Hua K,et al.Soil sickness of peanuts is attributable to modifications in soil microbes induced by peanut root exudates rather than to direct allelopathy[J].Soil Biology&Biochemistry,2014,78:149-159.
[33]Liu J,Wang X,Zhang T,et al.Assessment of active bacteria metabolizing phenolic acids in the peanut(Arachis hypogaea L.)rhizosphere[J].Microbiological Research,2017,205:118-124.
[34]Li X,Boer W D,Zhang Y,et al.Suppression of soil-borne Fusarium pathogens of peanut by intercropping with the medicinal herb Atractylodes lancea[J].Soil Biology&Biochemistry,2018,116:120-130.
[35]刘艳霞,李想,蔡刘体,等.烟草根系分泌物酚酸类物质的鉴定及其对根际微生物的影响[J].植物营养与肥料学报,2016,22(2):418-428.
[36]王璞,赵秀琴.几种化感物质对棉花种子萌发及幼苗生长的影响[J].中国农业大学学报,2001,6(3):26-31.
[37]沈荔花.外来植物加拿大一枝黄花(Solidagocanadensis L.)入侵的化感作用机制研究[D].福州:福建农林大学,2007.
[2]Bozhinova R.Effect of long-term potassium fertilization on the chemical composition of Oriental tobacco[J].Journal of Central European Agriculture,2012,13(3):510-518.
[3]贾健,朱金峰,杜修智,等.不同种植模式对烤烟根际土壤微生物、土壤养分和烟叶质量的影响[J].西南农业学报,2016,29(10):2300-2306.
[4]She S,Niu J,Zhang C,et al.Significant relationship between soil bacterial community structure and incidence of bacterial wilt disease under continuous cropping system[J].Archives of Microbiology,2017,199(2):1-9.
[5]岳冰冰.烤烟连作改变了根际土壤微生物的多样性[D].哈尔滨:东北林业大学,2012.
[6]张翼,张长华,王振民,等.连作对烤烟生长和烟地土壤酶活性的影响[J].中国农学通报,2007,23(12):211-215.
[7]李鑫,张秀丽,孙冰玉,等.烤烟连作对耕层土壤酶活性及微生物区系的影响[J].土壤,2012,44(3):456-460.
[8]邓阳春,黄建国.长期连作对烤烟产量和土壤养分的影响[J].植物营养与肥料学报,2010,16(4):840-845.
[9]邵孝侯,刘旭,周永波,等.生物有机肥改良连作土壤及烤烟生长发育的效应[J].中国土壤与肥料,2011,(2):65-67.
[10]Chen S L,Zhou B L,Lin S S,et al.Accumulation of cinnamic acid and vanillin in eggplant root exudates and the relationship with continuous cropping obstacle[J].African Journal of Biotechnology,2011,10(14):2659-2665.
[11]Xiao C L,Zheng J H,Zou L Y.Autotoxic effects of root exudates of soybean[J].Allelopathy Journal,2006,18(1):121-127.
[12]Liu P,Liu Z H,Wang C B,et al.Effects of three longchain fatty acids present in peanut(Arachishypogaea L.)root exudates on its own growth and the soil enzymes activities[J].Allelopathy Journal,2012,29(1):13-24.
[13]Liu P,Wan S B,Jiang L H.Autotoxic potential of root exudates of peanut(Arachishypogaea L.)[J].Allelopathy Journal,2010,26(2):197-206.
[14]Qu X H,Wang J G.Effect of amendments with different phenolic acids on soil microbial biomass,activity,and community diversity[J].Applied Soil Ecology,2008,39(2):172-179.
[15]Lu X F,Zhang H,Lyu S S,et al.Effects of exogenous phenolic acids on photosystem functions and photosynthetic electron transport rate in strawberry leaves[J].Photosynthetica,2018,56(2):616-622.
[16]符建国,易建华,贾志红,等.轮作与连作烟田根际土壤酸性有机组分的初步分离与鉴定[J].中国烟草科学,2011,32(6):67-71.
[17]陈冬梅,杨宇虹,晋艳,等.连作烤烟根际土壤自毒物质成分分析[J].草业科学,2011,28(10):1766-1769.
[18]陈冬梅,黄锦文,柯文辉,等.连作烟草根际土壤化感潜力评价及化感物质鉴定[J].中国烟草学报,2012,18(1):46-52.
[19]尤垂淮,高峰,王峰吉,等.连作对云南烤烟根际微生态及烟叶产质量的影响[J].中国烟草学报,2015,21(1):60-67.
[20]刘艳霞.土传烟草青枯病的生物防控及其机理研究[D].南京:南京农业大学,2012.
[21]吴文祥.烟草自毒物质及其对根际土壤微生物影响的研究[D].福州:福建农林大学,2010.
[22]徐婷,沈方科,石保峰,等.烟草根系分泌的酚酸及毒性[C]//广西烟草学会2013年学术年会,2013.
[23]张克勤,徐婷,沈方科,等.烟草根系分泌的酚酸及自毒效应[J].西南农业学报,2013,26(6):2552-2557.
[24]Greven S,Kneib T.Noncrossing quantile regression curve estimation[J].Biometrika,2010,97(4):825.
[25]Blum U.Allelopathic interactions involving phenolic acids[J].Journal of Nematology,1996,28(3):259-267.
[26]李亮亮,李天来,张恩平,等.四种酚酸物质在土壤中降解的研究[J].土壤通报,2010,(6):1460-1465.
[27]Song D.Who says rookie not data analysis(SPSS)(full color)(Chinese Edition)[M].Beijing:Publishing House of Electronic Industry,2016.
[28]李培栋,王兴祥,李奕林,等.连作花生土壤中酚酸类物质的检测及其对花生的化感作用[J].生态学报,2010,30(8):2128-2134.
[29]孙海兵,毛志泉,朱树华.环渤海湾地区连作苹果园土壤中酚酸类物质变化[J].生态学报,2011,31(1):90-97.
[30]李贺勤,刘奇志,张林林,等.草莓连作土壤酚酸类物质积累对土壤线虫的影响[J].生态学杂志,2014,33(1):169-175.
[31]谭秀梅,王华田,孔令刚,等.杨树人工林连作土壤中酚酸积累规律及对土壤微生物的影响[J].山东大学学报(理学版),2008,43(1):14-19.
[32]Li X G,Ding C F,Hua K,et al.Soil sickness of peanuts is attributable to modifications in soil microbes induced by peanut root exudates rather than to direct allelopathy[J].Soil Biology&Biochemistry,2014,78:149-159.
[33]Liu J,Wang X,Zhang T,et al.Assessment of active bacteria metabolizing phenolic acids in the peanut(Arachis hypogaea L.)rhizosphere[J].Microbiological Research,2017,205:118-124.
[34]Li X,Boer W D,Zhang Y,et al.Suppression of soil-borne Fusarium pathogens of peanut by intercropping with the medicinal herb Atractylodes lancea[J].Soil Biology&Biochemistry,2018,116:120-130.
[35]刘艳霞,李想,蔡刘体,等.烟草根系分泌物酚酸类物质的鉴定及其对根际微生物的影响[J].植物营养与肥料学报,2016,22(2):418-428.
[36]王璞,赵秀琴.几种化感物质对棉花种子萌发及幼苗生长的影响[J].中国农业大学学报,2001,6(3):26-31.
[37]沈荔花.外来植物加拿大一枝黄花(Solidagocanadensis L.)入侵的化感作用机制研究[D].福州:福建农林大学,2007.
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