马齿苋总黄酮的超声波辅助提取工艺优化及其抗氧化、抗肿瘤活性研究
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摘要
优化马齿苋中总黄酮的甲醇浸提-超声波辅助提取最佳工艺,并评价其体外抗氧化、抗肿瘤活性。以甲醇浓度、提取温度、料液比和超声功率为因素,以总黄酮提取率为指标,通过单因素试验与正交设计优选马齿苋总黄酮的超声波辅助提取条件;测定其对DPPH·和·OH的清除能力评价抗氧化活性;MTT法测定其对人肺癌细胞A549体外增殖的影响。结果表明:马齿苋总黄酮的最佳提取工艺为:料液比1:50,温度80℃,超声功率200 W,甲醇浓度90%,提取30 min,提取2次,总黄酮提取率为6.37%。马齿苋总黄酮对DPPH·清除率在4.0 mg/mL为89.80%,对·OH清除率在20.0 mg/mL为86.07%;其质量浓度为420μg/mL时对细胞A549增殖的抑制率为74.62%,与阳性药组无显著性差异。该优化工艺准确可靠,提取率高;马齿苋总黄酮具有较强的抗氧化和抗肿瘤活性。
To explore the optimum extraction process of flavonoids from Portulaca oleracea L. by methanol leaching-ultrasonic assistance and evaluate its antioxidant and anti-cancer activities. Based on single factor experiment, methanol concentration, extraction temperature, solid-liquid ratio and ultrasonic power were enrolled in this experiment, and the extracting ratio of total flavonoids was used to evaluate the extraction process. The extraction process of total flavonoids from P. oleracea L. was optimized by orthogonal experiment.Meanwhile, the antioxidant activity of flavonoids was assessed by scavenging assays of DPPH· and ·OH. Theviability of human lung cell line A549 was detected by MTT assay. The optimal extraction process was asfollows: liquid-solid ratio of 1:50, water bath temperature of 80℃, ultrasonic power of 200 W, 90% methanolas extraction solvent, extraction time of 30 min, extraction of 2 times. The extraction rate of total flavonoidsfrom P. oleracea L. was 6.37% under the optimal condition. The scavenging rate of DPPH · was 89.80% at4.0 mg/mL, while the scavenging rate of · OH was 86.07% at 20 mg/mL. The inhibition rate of cell A549 proliferation was 74.62% at 420 μg/mL, which had no significant difference from that of the positive druggroup. The flavonoids from P. oleracea L. are extracted efficiently by the above optimized technology and the total flavonoids exhibit antioxidant and antitumor activities.
To explore the optimum extraction process of flavonoids from Portulaca oleracea L. by methanol leaching-ultrasonic assistance and evaluate its antioxidant and anti-cancer activities. Based on single factor experiment, methanol concentration, extraction temperature, solid-liquid ratio and ultrasonic power were enrolled in this experiment, and the extracting ratio of total flavonoids was used to evaluate the extraction process. The extraction process of total flavonoids from P. oleracea L. was optimized by orthogonal experiment.Meanwhile, the antioxidant activity of flavonoids was assessed by scavenging assays of DPPH· and ·OH. Theviability of human lung cell line A549 was detected by MTT assay. The optimal extraction process was asfollows: liquid-solid ratio of 1:50, water bath temperature of 80℃, ultrasonic power of 200 W, 90% methanolas extraction solvent, extraction time of 30 min, extraction of 2 times. The extraction rate of total flavonoidsfrom P. oleracea L. was 6.37% under the optimal condition. The scavenging rate of DPPH · was 89.80% at4.0 mg/mL, while the scavenging rate of · OH was 86.07% at 20 mg/mL. The inhibition rate of cell A549 proliferation was 74.62% at 420 μg/mL, which had no significant difference from that of the positive druggroup. The flavonoids from P. oleracea L. are extracted efficiently by the above optimized technology and the total flavonoids exhibit antioxidant and antitumor activities.
引文
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[16]翟硕莉.超声波法和微波法提取马齿苋中总黄酮的比较研究[J].中国酿造,2012,31(8):120-122.
[17]赵文英,陈丽娜,徐艳丽,等.马齿苋黄酮提取方法比较及工艺优化[J].青岛科技大学学报:自然科学版,2017,38(2):38-41.
[18]陈国妮,孙飞龙,闫亚茹.马齿苋类黄酮提取工艺及抑菌效果的研究[J].包装与食品机械,2016,34(1):6-10.
[19]翟硕莉.超临界CO2萃取马齿苋中总黄酮的工艺研究[J]北方园艺,2012(12):45-47.
[20]李玉萍,曾宪伟,叶军,等.马齿苋活性成分体内外抗癌作用的初步筛选[J].时珍国医国药,2009,20(11):2726-2728.
[21]陈国妮,孙飞龙,闫亚茹.马齿苋总黄酮的提取纯化工艺研究[J].中国饲料,2015(23):26-29.
[22]陈智坤,梁呈元,李维林,等.薄荷不同溶剂提取物抗氧化活性的研究[J].食品工业科技,2013,34(3):100-103.
[23]汪荔,王征,张娇,等.传统工艺与膜分离技术联合对马齿苋多糖的提取分离与抗氧化活性研究[J].中草药,2016,47(10):1676-1681.
[24]郝红英,牛美兰,王研,等.乙醇-硫酸铵双水相提取马齿苋总黄酮工艺研究[J].安徽农业科学,2016,44(11):145-147.
[2]冯彦.马齿苋的药理作用及营养保健作用[J].中医临床研究,2017,9(3):117-118.
[3]金天云,沈涛,周明星,等.马齿苋的化学成分及其生物活性研究[J].中国药学:英文版,2016,25(12):898-905.
[4]王天宁,刘玉婷,肖凤琴,等.马齿苋化学成分及药理活性的现代研究整理[J].中国实验方剂学杂志,2018,24(6):224-234.
[5]雷红伟,陆付耳,徐丽君,等.紫外分光光度法测定马齿苋总黄酮的含量[J].中西医结合研究,2010,2(3):126-128.
[6]郑智音,贾晓斌,舒娈,等.鲜马齿苋多糖、生物碱和多酚类组分的制备及其降血糖活性研究[J].中草药,2014,45(18):2673-2677.
[7]陈国妮,孙飞龙,闫亚茹.马齿苋类黄酮提取工艺及抑菌效果的研究[J].包装与食品机械,2016,34(1):6-10.
[8]詹雁,王立珍,徐超群,等.马齿苋不同提取部位抗氧化活性的比较[J].中药药理与临床,2012,28(5):126-128.
[9]谢晓凤,童莲花,童德胜,等.马齿苋总黄酮的提取及其浓缩汁抗氧化性研究[J].食品科技,2013,38(2):192-197.
[10]潘晓丽,熊永爱,谭玉柱,等.马齿苋总黄酮对肝纤维化大鼠转化生长因子-β1信号因子表达的影响[J].医药导报,2014,33(9):1140-1143.
[11]崔丽敏,张学武.马齿苋提取物抑制肺癌A549细胞增殖的实验研究[J].四川中医杂志,2007,25(12):15-16.
[12]Boskabady M H,Hashemzehi M,Khazdair M R,et al.Hydroethanolic extract of Portulaca oleracea affects Beta-adrenoceptors of Guinea pig tracheal smooth muscle[J].Iranian Journal of Pharmaceutical Research Ijpr,2016,15(4):867-874.
[13]Yue T,Wen X S,Qi R R,et al.The effects of Portulaca oleracea on hypoxia-induced pulmonary edema in mice[J].High Altitude Medicine&Biology,2015,16(1):43-51.
[14]Eidi A,Mortazavi P,Moghadam J Z,et al.Hepatoprotective effects of Portulaca oleracea extract against CCl4-induced damage in rats[J].Pharmaceutical Biology,2014,53(7):1042-1051.
[15]李亚,金晶,杨艳,等.马齿苋中总黄酮提取工艺优化及其抗氧化活性研究[J].化学与生物工程,2015,32(4):33-37.
[16]翟硕莉.超声波法和微波法提取马齿苋中总黄酮的比较研究[J].中国酿造,2012,31(8):120-122.
[17]赵文英,陈丽娜,徐艳丽,等.马齿苋黄酮提取方法比较及工艺优化[J].青岛科技大学学报:自然科学版,2017,38(2):38-41.
[18]陈国妮,孙飞龙,闫亚茹.马齿苋类黄酮提取工艺及抑菌效果的研究[J].包装与食品机械,2016,34(1):6-10.
[19]翟硕莉.超临界CO2萃取马齿苋中总黄酮的工艺研究[J]北方园艺,2012(12):45-47.
[20]李玉萍,曾宪伟,叶军,等.马齿苋活性成分体内外抗癌作用的初步筛选[J].时珍国医国药,2009,20(11):2726-2728.
[21]陈国妮,孙飞龙,闫亚茹.马齿苋总黄酮的提取纯化工艺研究[J].中国饲料,2015(23):26-29.
[22]陈智坤,梁呈元,李维林,等.薄荷不同溶剂提取物抗氧化活性的研究[J].食品工业科技,2013,34(3):100-103.
[23]汪荔,王征,张娇,等.传统工艺与膜分离技术联合对马齿苋多糖的提取分离与抗氧化活性研究[J].中草药,2016,47(10):1676-1681.
[24]郝红英,牛美兰,王研,等.乙醇-硫酸铵双水相提取马齿苋总黄酮工艺研究[J].安徽农业科学,2016,44(11):145-147.
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