蜡样芽孢杆菌CP-1对Cr(Ⅵ)还原效果研究
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摘要
为了提高蜡样芽孢杆菌CP-1菌株对Cr(Ⅵ)的还原效果,采用单因素和正交试验,通过摇瓶发酵培养,对影响蜡样芽孢杆菌CP-1菌株还原Cr(Ⅵ)的发酵培养基成分和培养条件进行了优化,并研究了最佳发酵条件下的蜡样芽孢杆菌CP-1对Cr(Ⅵ)的还原效果。结果表明,蜡样芽孢杆菌CP-1菌株还原Cr(Ⅵ)的最佳培养基组成为:1%甘露醇, 3%的大豆蛋白胨, 0.05%KCl, 0.1%CuSO4,在此基础上的最佳培养条件为:pH7.0、6%接种量、45℃培养3 d,在此条件下,Cr(Ⅵ)初始浓度为100mg·L~(-1)时,对Cr(Ⅵ)的还原率达99.75%。在Cr(Ⅵ)污染的土壤中添加蜡样芽孢杆菌CP-190d后,土壤中的Cr(Ⅵ)含量降低55.15%左右。
In order to improve the Cr(Ⅵ) reduction by Bacillus cereus CP-1, single-factor and orthogonal experiments were used to study the effects of fermentation and culture conditions on Cr(Ⅵ) reduction, and the reduction effects of B. cereus CP-1 on Cr(Ⅵ) were evaluated under the optimal fermentation conditions. The results showed that the optimal medium for the Cr(Ⅵ) reduction by B. cereus CP-1 was 1% mannitol, 3% soy peptone, 0.05% KCl and 0.1% CuSO4. The reduction rate of Cr(Ⅵ) was 99.75% based on the best culture condition of pH7, 6% inoculation amount and 45℃ for 3 d when the initial concentration of Cr(Ⅵ) was 100 mg·L~(-1). Specially, the Cr(Ⅵ) content in Cr(Ⅵ)-contaminated soil was decreased 55.15%after adding B. cereus CP-1 for 90 d.
In order to improve the Cr(Ⅵ) reduction by Bacillus cereus CP-1, single-factor and orthogonal experiments were used to study the effects of fermentation and culture conditions on Cr(Ⅵ) reduction, and the reduction effects of B. cereus CP-1 on Cr(Ⅵ) were evaluated under the optimal fermentation conditions. The results showed that the optimal medium for the Cr(Ⅵ) reduction by B. cereus CP-1 was 1% mannitol, 3% soy peptone, 0.05% KCl and 0.1% CuSO4. The reduction rate of Cr(Ⅵ) was 99.75% based on the best culture condition of pH7, 6% inoculation amount and 45℃ for 3 d when the initial concentration of Cr(Ⅵ) was 100 mg·L~(-1). Specially, the Cr(Ⅵ) content in Cr(Ⅵ)-contaminated soil was decreased 55.15%after adding B. cereus CP-1 for 90 d.
引文
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[22]SHAKOORI A R,MAKHDOOM M,HAQ R U.Hexavalent chromium reduction by a dichromate-resistant gram-positive bacterium isolated from effluents of tanneries[J].Applied Microbiology and Biotechnology,2000,53(3):348-351.
[2]KANMANI P,ARAVIND J,PRESTON D.Remediation of chromium contaminants using bacteria[J].International Journal of Environmental Science&Technology,2012,9(1):183-193.
[3]DONMEZ G and KOCBERBER N.Bioaccumulation of hexavalent chromium by enriched microbial cultures obtained from molasses and NaCl containing media[J].Process Biochemistry,2005,40(7):2493-2498.
[4]ZHAQ C,YANG Q,CHEN W,et al.Removal of hexavalent chromium in tannery wastewater by Bacillus cereus[J].Canadian Journal of Microbiology,2012,58(1):23-28.
[5]焦仕林,朱培蕾,姜朴,等.蜡样芽孢杆菌还原六价铬效果分析[J].中国公共卫生,2016,32(10):1326-1329
[6]AMOOZEGAR M A,GHASEMI A,RAZAVI M R,et al.Evaluation of Hexavalent chromium reduction by chromate resistant moderate halophile Nesterenkonia sp.strain MF2[J].Process Biochemistry,2007,42(10):1475-1479.
[7]SONI S K,SINGH R,AWASTHI A,et al.A Cr(VI)-reducing Microbacterium sp.strain SUCR140 enhances growth and yield of Zea mays in Cr(VI)amended soil through reduced chromium toxicity and improves colonization of arbuscular mycorrhizal fungi[J].Environmental Science and Pollution Research,2014,21(3):1971-1979.
[8]HE Zhiguo,GAO Fengling,SHA Tao,et al.Isolation and characterization of a Cr(VI)reduction Ochrobactrum sp.strain CSCr-3 from chromium landfill[J].Journal of Hazardous Materials,2009,163(2/3):869-873.
[9]VILLEGAS L B,FERNANDEZ P M,AMOROSO M J,et al.Chromate removal by yeasts isolated from sediment of a tanning factory and a mine site in Argentina[J].Biometals,2008,21(5):591-600.
[10]ELANGOVAN R,PHILIP L and CHANDRARAJ K.Hexavalent chromium reduction by free and immobilized cell-free extract of Arthrobacter rhombi-RE[J].Applied Biochemistry and Biotechnology,2010,160(1):81-97.
[11]THACKER U,PARIKH R,SHOUCHE Y,et al.Reduction of chromate by cell-free extract of Brucella sp.isolated from Cr(VI)contaminated sites[J].Bioresource Technology,2007,98(8):1541-1547.
[12]HE Da,ZHENG Maosheng,MA Tao,et al.Interaction of Cr(VI)reduction and denitrification by strain Pseudomonas aeruginosa PCN-2 under aerobic conditions[J].Bioresource Technology,2015,185:346-352.
[13]SRINATH T,VERMA T,RAMTEKE P W.Cr(VI)biosorption and bioaccumulation by chromate resistant bacteria[J].Chemosphere,2002,48(4):427-435.
[14]ABOU-SHANAB R A I,ANGLE J S,VAN-BERKUM P.Chromate tolerant bacteria for enhanced metal uptake by Eichhornia crassipes(MART)[J].International Journal of Phytoremediation,2007,9(2):91-105.
[15]BAI R S,ABRAHAM T E.Studies on Chromium(VI)adsorption-desorption using immobilized fungal biomass[J].Bioresource Technology,2003,87(1):17-26.
[16]国家环境保护总局.水和溶液检测分析方法[M].北京:中国环境科学出版社,2002:344-351.
[17]黄世臣,李熙英,王娟.高效吸附铬的菌株筛选及优化条件[J].东北林业大学学报,2010,38(4):105-106.
[18]ZHOU Ximin,DONG Lanlan,DENG Peng,et al.Reducing capacity and enzyme activity of chromate reductase in a ChrT-engineered strain[J].Experimental and Therapeutic Medicine,2017,14(3):2361-2366.
[19]MCLEAN J,BEVERIDGE T J,PHIPPS D.Isolation and characterization of a chromium-reducing bacterium from a chromated copper arsenate contaminated site[J].Environmental Microbiology,2000,2(6):611-619.
[20]杨文玲,王继雯,慕琦,等.耐Cr(VI)菌株的筛选及条件优化[J].河南科学,2013,31(8):1175-1179.
[21]LIU Yunguo,XU Weihua,ZENG Guangming.Experimental study on reduction Cr(VI)by Pseudomonas aeruginosa[J].Journal of Environmental Sciences,2004,16(5):797-801.
[22]SHAKOORI A R,MAKHDOOM M,HAQ R U.Hexavalent chromium reduction by a dichromate-resistant gram-positive bacterium isolated from effluents of tanneries[J].Applied Microbiology and Biotechnology,2000,53(3):348-351.
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