红树林菌株对毛用活性红染料的降解和脱色研究
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摘要
通过梯度驯化,从土壤中分离筛选出能以毛用活性红PW-6g为唯一碳源的4株菌株,对其进行偶氮还原酶、苯胺双加氧酶、苯甲酸-1,2-双加氧酶、邻苯二酚-1,2-双加氧酶、邻苯二酚-2,3-双加氧酶活性测定,确定综合酶活性较好的菌株,鉴定其为Bacillus subtilis spp.菌株。对该菌株的脱色反应条件进行研究,结果表明,当PW-6g初始质量浓度为600 mg/L时,在pH为7.5、温度为35℃、接种量为4%、盐质量浓度小于40 g/L的条件下,Mabhk-3脱色48 h对PW-6g的脱色降解率可接近100%。通过HPLC分析,均检测不出脱色液中含有苯二胺、苯胺、邻苯二甲酸、苯甲酸和邻苯二酚,达到排放要求。
Through gradient acclination,four strains that could use a kind of lanasol active red dye(PW-6 g) as sole carbon source were screened from natural mangrove soil. Of them,the activities of azoreductase,aniline dioxygenase,benzoate-1,2-dioxygenase,catechol-1,2-dioxygenase and catechol-2,3-dioxygenase were measured. Based on the prominent comprehensive enzymes activities,the strain was selected and identified as Bacillus subtilis spp.. The results from the study showed that the optimum initial concentration of PW-6 g was 600 mg/L,the optimum temperature for decolorization was 35 ℃,optimum pH was 7.5,optimum inoculating dose was 4%,and salt concentration was less than 40 g/L. Under the optimum conditions,the decolorization and degradation rate was nearly 100% after the treatment of PW-6 g with the strain Mabhk-3. It was found that p-phenylenediamine,aniline,phthalic acid,benzoic acid and catechol in the decolorization solution can not be detected by HPLC analysis and the decolorization solution met the emission specifications.
Through gradient acclination,four strains that could use a kind of lanasol active red dye(PW-6 g) as sole carbon source were screened from natural mangrove soil. Of them,the activities of azoreductase,aniline dioxygenase,benzoate-1,2-dioxygenase,catechol-1,2-dioxygenase and catechol-2,3-dioxygenase were measured. Based on the prominent comprehensive enzymes activities,the strain was selected and identified as Bacillus subtilis spp.. The results from the study showed that the optimum initial concentration of PW-6 g was 600 mg/L,the optimum temperature for decolorization was 35 ℃,optimum pH was 7.5,optimum inoculating dose was 4%,and salt concentration was less than 40 g/L. Under the optimum conditions,the decolorization and degradation rate was nearly 100% after the treatment of PW-6 g with the strain Mabhk-3. It was found that p-phenylenediamine,aniline,phthalic acid,benzoic acid and catechol in the decolorization solution can not be detected by HPLC analysis and the decolorization solution met the emission specifications.
引文
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[11] Xu G R,Zhang Y P,Li G B,et al. Degradation of azo dye active brilliant red X-3B by composite ferrate solution[J]. Journal of Hazardous Materials,2009,161(2/3):1299-1305.
[12]刘志培,杨惠芳,周培谨.微生物降解苯胺的特性及其降解代谢途径[J].应用与环境微生物学报,1999,5(Z1):5-9.
[2]章杰.毛用活性染料的发展和应用[J].染整技术,2014,36(3):43-48.
[3] Ali H. Biodegradation of synthetic dyes-a review[J]. Water Air and Soil Pollution,2010,213(1/2/3/4):251-273.
[4] Alvarez M S,Moscoso F,Rodríguez A,et al. Novel physico-biological treatment for the remediation of textile dyes-containing industrial effluents[J]. Bioresource Technology,2013,146(4):689-695.
[5] Kurade M B,Waghmode T R,Jadhav M U,et al. Bacterial-yeast consortium as an effective biocatalyst for biodegradation of sulphonated azo dye Reactive Red 198[J]. RSC Advances,2015,5(29):23046-23056.
[6] Chen Y,Feng L,Li H,et al. Biodegradation and detoxification of Direct Black G textile dye by a newly isolated thermophilic microflora[J]. Bioresource Technology,2018,250:650-657.
[7]花莉,解井坤,朱超,等.微生物对偶氮染料脱色降解的研究进展[J].西北农林科技大学学报(自然科学版),2015,43(10):103-113.
[8]司美茹,苏涛,杨革.一株高效广谱染料降解细菌的分离鉴定及脱色特性研究[J].中国生物工程杂志,2010,30(6):70-76.
[9]冉华文.天津德凯公司系列毛用活性染料[J].染料与染色,2006,43(5):15,60.
[10]都林娜,李刚,王阳,等.细菌降解偶氮染料的研究进展[J].工业水处理,2015,35(12):1-4.
[11] Xu G R,Zhang Y P,Li G B,et al. Degradation of azo dye active brilliant red X-3B by composite ferrate solution[J]. Journal of Hazardous Materials,2009,161(2/3):1299-1305.
[12]刘志培,杨惠芳,周培谨.微生物降解苯胺的特性及其降解代谢途径[J].应用与环境微生物学报,1999,5(Z1):5-9.
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