中长链脂肪酸为碳源的Pseudomonas veronii和Pseudomonas alcaliphila反硝化途径亚硝酸盐累积特性
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摘要
厌氧-厌氧氨氧化组合工艺作为低能耗脱氮工艺,如何提供适宜比例的亚硝酸盐成为研究的关键问题之一.部分反硝化为稳定提供厌氧氨氧化所需的亚硝酸盐提供了可行途径.本文重点针对厌氧工艺中可能产生的中长链脂肪酸对反硝化过程的影响进行研究,筛选出两株具有反硝化能力的细菌Pseudomonas veronii(W-22)和Pseudomonas alcaliphila(W-39),通过批次试验,考察了中长链脂肪酸和常用碳源对菌株反硝化性能的影响.结果表明,在硝酸盐浓度为100 mg·L~(-1),C/N=15,30℃条件下,W-22利用葡萄糖、W-39利用乙醇和葡萄糖,可在36 h内达到稳定的亚硝酸盐累积,亚硝酸盐最大累积速率(R_m)分别为2.50、5.56和8.35 mg·L~(-1)·h~(-1),亚硝酸盐浓度分别维持在57.11、82.14和80.16 mg·L~(-1);W-39利用己酸钠为碳源的R_m为0.99 mg·L~(-1)·h~(-1),亚硝酸盐浓度逐渐升高至72.34 mg·L~(-1);W-22和W-39利用辛酸钠的反应迟滞期在57 h以上,后期伴随硝酸盐浓度降低和亚硝酸盐浓度升高,R_m分别为0.97和7.17 mg·L~(-1)·h~(-1).在本研究条件下,碳源类型对菌株反硝化进程的影响存在差异.
The appropriate nitrite proportion has become one of the key issues for anaerobic-ANAMMOX process, and a feasible way to provide stable nitrite for ANAMMOX is via partial denitrification. This study was investigated on the influence of fatty acids as carbon sources on the denitrification process. Strain W-22 and W-39 with denitrification ability were isolated and were identified as Pseudomonas veronii and Pseudomonas alcaliphila, respectively. Under the condition that initial nitrate concentration was 100 mg·L~(-1), C/N=15 and the operation temperature was 30 ℃, stable nitrite accumulation can be observed within 36 h for W-22 using glucose, and for W-39 using both ethanol and glucose, with the maximum accumulation rate(R_m) of 2.50、5.56 and 8.35 mg·L~(-1)·h~(-1), and the nitrite concentration were 57.11, 82.14 and 82.16 mg·L~(-1),respectively. When sodium caproate was used in W-39, the nitrite concentration gradually increased to 72.34 mg·L~(-1) with the R_m of 0.99 mg·L~(-1)·h~(-1). When sodium caprylate was used for W-22 and W-39, the lag phase was up to 57 h, followed by the nitrite concentration increased with the R_m were 0.97 and 7.17 mg·L~(-1)·h~(-1). The influence of carbon source on denitrification process of bacterial strain was different.
The appropriate nitrite proportion has become one of the key issues for anaerobic-ANAMMOX process, and a feasible way to provide stable nitrite for ANAMMOX is via partial denitrification. This study was investigated on the influence of fatty acids as carbon sources on the denitrification process. Strain W-22 and W-39 with denitrification ability were isolated and were identified as Pseudomonas veronii and Pseudomonas alcaliphila, respectively. Under the condition that initial nitrate concentration was 100 mg·L~(-1), C/N=15 and the operation temperature was 30 ℃, stable nitrite accumulation can be observed within 36 h for W-22 using glucose, and for W-39 using both ethanol and glucose, with the maximum accumulation rate(R_m) of 2.50、5.56 and 8.35 mg·L~(-1)·h~(-1), and the nitrite concentration were 57.11, 82.14 and 82.16 mg·L~(-1),respectively. When sodium caproate was used in W-39, the nitrite concentration gradually increased to 72.34 mg·L~(-1) with the R_m of 0.99 mg·L~(-1)·h~(-1). When sodium caprylate was used for W-22 and W-39, the lag phase was up to 57 h, followed by the nitrite concentration increased with the R_m were 0.97 and 7.17 mg·L~(-1)·h~(-1). The influence of carbon source on denitrification process of bacterial strain was different.
引文
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Heylen K,Gevers D,Vanparys B,et al.2006.The incidence of nirS and nirK and their genetic heterogeneity in cultivated denitrifiers[J].Environmental Microbiology,8(11):2012-2021
Kartal B,Kuenen J G,van Loosdrecht M C M.2010.Sewage Treatment with Anammox[J].Science,328(5979):702-703
康鹏亮,陈胜男,黄廷林,等.2018.好/厌氧条件下反硝化细菌脱氮特性与功能基因[J].环境科学,39(8):3789-3796
Lackner S,Gilbert E M,Vlaeminck S E,et al.2014.Full-scale partial nitritation/anammox experiences——an application survey[J].Water Research,55:292-303
Ma B,Wang S,Cao S,et al.2016.Biological nitrogen removal from sewage via anammox:Recent advances[J].Bioresource Technology,200:981-990
Miao H,Wang S,Zhao M,et al.2014.Codigestion of Taihu blue algae with swine manure for biogas production[J].Energy Conversion & Management,77(1):643-649
Palleroni N J,Doudoroff M,Stanier R Y,et al.1970.Taxonomy of the aerobic Pseudomonads:the properties of the pseudomonas stutzeri group[J].Microbiology,60(2):215-231
Philippot L.2002.Denitrifying genes in bacterial and Archaeal genomes[J].BBA-Gene Structure and Expression,1577(3):355-376
Si Z,Peng Y,Yang A,et al.2018.Rapid nitrite production via partial denitrification:pilot-scale operation and microbial community analysis[J].Environmental Science:Water Research & Technology,4(1):80-86
Strous M,Heijnen J J,Kuenen J G,et al.1998.The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammonium-oxidizing microorganisms[J].Applied Microbiology and Biotechnology,50(5):589-596
邵留,徐祖信,尹海龙.2007.污染水体脱氮工艺中外加碳源的研究进展[J].工业水处理,27(12):10-14
沈萍,陈向东.2007.微生物学实验[M].北京:高等教育出版社
Torà J A,Baeza J A,Carrera J,et al.2011.Denitritation of a high-strength nitrite wastewater in a sequencing batch reactor using different organic carbon sources[J].Chemical Engineering Journal,172(2/3):994-998
王淑莹,殷芳芳,侯红勋,等.2009.以甲醇作为外碳源的生物反硝化[J].北京工业大学学报,35(11):1521-1526
徐亚同.1994.不同碳源对生物反硝化的影响[J].环境科学,(2):29-32+44+93
徐亚同.1995.挥发性脂肪酸碳源生物反硝化研究[J].华东师范大学学报(自然科学版),(2):70-76
阎宁,金雪标,张俊清.2002.甲醇与葡萄糖为碳源在反硝化过程中的比较[J].上海师范大学学报(自然科学版),(3):41-44
燕艳,邹德勋,王小伟,等.2013.餐厨垃圾厌氧消化残余物污染特征分析[C].2013中国环境科学学会学术年会,昆明
张永梅,王晓昌,程喆,等.2017.厨余垃圾短程发酵产物的性质及其反硝化性能[J].环境工程学报,11(5):3017-3023
曾庆武,梁运祥,葛向阳.2008.反硝化细菌的分离筛选及其反硝化特性的初步研究[J].华中农业大学学报,27(5):616-620
丁伟军.2018.纤维素酶解反应动力学的研究[J].化工与医药工程,39(4):8-14
Elomari M,Coroler L,Hoste B,et al.1996.DNA relatedness among pseudomonas strains isolated from natural mineral waters and proposal of pseudomonas veronii sp.nov[J].International Journal of Systematic and Evolutionary Microbiology,46(4):1138-1144
Gómez M A,González-López J,Hontoria-Garci A E.2000.Influence of carbon source on nitrate removal of contaminated groundwater in a denitrifying submerged filter[J].Journal of Hazardous Materials,80(1):69-80
国家环境保护总局.2002.水和废水监测分析方法[M].北京:中国环境科学出版社
Ge S,Peng Y,Wang S,et al.2012.Nitrite accumulation under constant temperature in anoxic denitrification process:The effects of carbon sources and COD/NO3-N[J].Bioresource Technology,114:137-143
Graaf A A V D,Mulder A,Bruijn P D,et al.1995.Anaerobic oxidation of ammonium is a biologically mediated process[J].Applied & Environmental Microbiolog,61(4):1246
Heylen K,Gevers D,Vanparys B,et al.2006.The incidence of nirS and nirK and their genetic heterogeneity in cultivated denitrifiers[J].Environmental Microbiology,8(11):2012-2021
Kartal B,Kuenen J G,van Loosdrecht M C M.2010.Sewage Treatment with Anammox[J].Science,328(5979):702-703
康鹏亮,陈胜男,黄廷林,等.2018.好/厌氧条件下反硝化细菌脱氮特性与功能基因[J].环境科学,39(8):3789-3796
Lackner S,Gilbert E M,Vlaeminck S E,et al.2014.Full-scale partial nitritation/anammox experiences——an application survey[J].Water Research,55:292-303
Ma B,Wang S,Cao S,et al.2016.Biological nitrogen removal from sewage via anammox:Recent advances[J].Bioresource Technology,200:981-990
Miao H,Wang S,Zhao M,et al.2014.Codigestion of Taihu blue algae with swine manure for biogas production[J].Energy Conversion & Management,77(1):643-649
Palleroni N J,Doudoroff M,Stanier R Y,et al.1970.Taxonomy of the aerobic Pseudomonads:the properties of the pseudomonas stutzeri group[J].Microbiology,60(2):215-231
Philippot L.2002.Denitrifying genes in bacterial and Archaeal genomes[J].BBA-Gene Structure and Expression,1577(3):355-376
Si Z,Peng Y,Yang A,et al.2018.Rapid nitrite production via partial denitrification:pilot-scale operation and microbial community analysis[J].Environmental Science:Water Research & Technology,4(1):80-86
Strous M,Heijnen J J,Kuenen J G,et al.1998.The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammonium-oxidizing microorganisms[J].Applied Microbiology and Biotechnology,50(5):589-596
邵留,徐祖信,尹海龙.2007.污染水体脱氮工艺中外加碳源的研究进展[J].工业水处理,27(12):10-14
沈萍,陈向东.2007.微生物学实验[M].北京:高等教育出版社
Torà J A,Baeza J A,Carrera J,et al.2011.Denitritation of a high-strength nitrite wastewater in a sequencing batch reactor using different organic carbon sources[J].Chemical Engineering Journal,172(2/3):994-998
王淑莹,殷芳芳,侯红勋,等.2009.以甲醇作为外碳源的生物反硝化[J].北京工业大学学报,35(11):1521-1526
徐亚同.1994.不同碳源对生物反硝化的影响[J].环境科学,(2):29-32+44+93
徐亚同.1995.挥发性脂肪酸碳源生物反硝化研究[J].华东师范大学学报(自然科学版),(2):70-76
阎宁,金雪标,张俊清.2002.甲醇与葡萄糖为碳源在反硝化过程中的比较[J].上海师范大学学报(自然科学版),(3):41-44
燕艳,邹德勋,王小伟,等.2013.餐厨垃圾厌氧消化残余物污染特征分析[C].2013中国环境科学学会学术年会,昆明
张永梅,王晓昌,程喆,等.2017.厨余垃圾短程发酵产物的性质及其反硝化性能[J].环境工程学报,11(5):3017-3023
曾庆武,梁运祥,葛向阳.2008.反硝化细菌的分离筛选及其反硝化特性的初步研究[J].华中农业大学学报,27(5):616-620
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