携Gelsolin单抗载紫杉醇PLGA微球对Hca-F荷瘤小鼠体内治疗及抑制淋巴结转移的研究
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摘要
目的制备一种以凝溶胶蛋白(gelsolin,GSN)为靶点载紫杉醇(paclitaxel,PTX)的PLGA (GSN-PTX-PLGA)微球,并研究其对Hca-F荷瘤小鼠体内治疗效果及抑制淋巴结转移的作用。方法采用4～6周龄雌性615小鼠建模将Hca-F细胞植入小鼠足垫,1周后观察小鼠成瘤情况。设对照组(生理盐水组)、PLGA微球组、PTX注射液组、PTX-PLGA微球组和GSN-PTX-PLGA微球组。分别对Hca-F细胞荷瘤小鼠尾静脉注射药物进行治疗、观察记录并绘制肿瘤体积生长曲线,给药4周后观察各组小鼠淋巴结转移情况。结果给药4周后GSN-PTX-PLGA微球组与PTX-PLGA微球组肿瘤生长曲线平缓,肿瘤体积较小与对照组比较(P<0.05),GSN-PTX-PLGA微球组小鼠淋巴结转移率(30%)与其他各组相比显著降低。结论 GSN-PTX-PLGA微球组与PTX-PLGA微球组对Hca-F细胞荷瘤小鼠的治疗有明显抑瘤效果,GSN-PTX-PLGA微球组对淋巴结转移有一定的抑制作用。
Objective To prepare a kind of gelsolin-targeted PLGA microspheres with paclitaxel loaded and to evaluate the treatment effect and the lymph node metastasis of Hca-F tumor-bearing mice.Methods The model of 615 female mice aged 4-6 weeks were used to implant the Hca-F cells into the mouse foot pad.The tumor growth of the mouse footpads were observed one week later.And the tail vein injection was done by using physiological saline,PLGA microspheres,paclitaxel,paclitaxel loaded PLGA(PTX-PLGA)microspheres and Gelsolin-targeted,paclitaxel loaded PLGA microspheres(GSN-PTX-PLGA).After intravenous injection of drugs for tumor formation in mice with Hca-F cells,the tumor growth curve was recorded and the lymph node metastasis of each group was observed after four weeks of treatment.Results After 4 weeks of treatment,the tumor size and volume in the group treated with GSNPTX-PLGA microspheres and PTX-PLGA microspheres were obviously decrease compared with other groups(P<0.05),and the lymph node metastasis rate in the group treated with GSN-PTX-PLGA microspheres was significantly less than those of other groups.Conclusions The GSN-PTX-PLGA microspheres and the PTX-PLGA microspheres have a significantly inhibitory effect on the tumors in the Hca-F cell bearing mice,and the GSN-PTX-PLGA microspheres has a certainly inhibition effect on lymph node metastasis.
Objective To prepare a kind of gelsolin-targeted PLGA microspheres with paclitaxel loaded and to evaluate the treatment effect and the lymph node metastasis of Hca-F tumor-bearing mice.Methods The model of 615 female mice aged 4-6 weeks were used to implant the Hca-F cells into the mouse foot pad.The tumor growth of the mouse footpads were observed one week later.And the tail vein injection was done by using physiological saline,PLGA microspheres,paclitaxel,paclitaxel loaded PLGA(PTX-PLGA)microspheres and Gelsolin-targeted,paclitaxel loaded PLGA microspheres(GSN-PTX-PLGA).After intravenous injection of drugs for tumor formation in mice with Hca-F cells,the tumor growth curve was recorded and the lymph node metastasis of each group was observed after four weeks of treatment.Results After 4 weeks of treatment,the tumor size and volume in the group treated with GSNPTX-PLGA microspheres and PTX-PLGA microspheres were obviously decrease compared with other groups(P<0.05),and the lymph node metastasis rate in the group treated with GSN-PTX-PLGA microspheres was significantly less than those of other groups.Conclusions The GSN-PTX-PLGA microspheres and the PTX-PLGA microspheres have a significantly inhibitory effect on the tumors in the Hca-F cell bearing mice,and the GSN-PTX-PLGA microspheres has a certainly inhibition effect on lymph node metastasis.
引文
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[7]于姣姣,赵云,刘朝奇,等.超声介导载紫杉醇微泡对小鼠H22皮下移植瘤抑制作用的实验研究[J].中国超声医学杂志,2016,32(9):833-836.
[2]QAZI A S,SUN M Z,HUANG Y H,et al.Subcellular proteomics:determination of specific location and expression levels of lymphatic metastasis associated proteins in hepatocellular carcinoma by subcellular fractionation[J].Biomed Pharmacother,2011,65(6):407-416.
[3]王薪懿,倪蓉,等.紫杉醇靶向微管的抗肿瘤机制的研究进展[J].国药学研究杂志,2012,3(1):56-60.
[4]NIU C,WANG Z,LU G,et al.Doxorubicin loaded superparamagnetic PLGA-iron oxide multifunctional microbubbles for dual-mode US/MR imaging and therapy of metastasis in lymph nodes[J].Biomaterials,2013,34(9):2307-2317.
[5]FIGUEIREDO M,ESENALIEV R.PLGA Nanoparticles for Ultrasound-Mediated Gene Delivery to Solid Tumors[J].Drug Deliv,2012,2012:767839.
[6]秦颢诚,武俊,周盟,等.携Gelsolin单抗靶向超声造影剂的制备及体外靶向实验[J].中国医学影像技术,2017,33(6):826-831.
[7]于姣姣,赵云,刘朝奇,等.超声介导载紫杉醇微泡对小鼠H22皮下移植瘤抑制作用的实验研究[J].中国超声医学杂志,2016,32(9):833-836.
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