赖氨酸席夫碱对进入绵羊小肠养分流量及瘤胃消化代谢的影响
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摘要
本文研究了赖氨酸席夫碱的体外抑菌活性,添喂赖氨酸、赖氨酸席夫碱对到达绵羊小肠蛋白和游离氨基酸流量及对绵羊瘤胃消化代谢的影响。
采用赖氨酸和水杨醛合成了赖氨酸席夫碱,并运用倍比稀释法进行大肠杆菌、金黄色葡萄球菌和沙门氏杆菌的最小抑菌活性试验,同时根据以上试验测定了在3倍最小抑菌浓度时的抑菌曲线,结果表明,赖氨酸席夫碱对上述试验菌的最小抑菌浓度分别为786.4、1228.8和983μg/mL,3倍最小抑菌曲线显示在4h后对各试验菌的抑菌活性趋于相近。
选用4只体重在40kg左右,安装永久性十二指肠瘘管的中国美利奴(新疆型)细毛羊,采用拉丁方设计,分别添加3.42g和6.84g赖氨酸席夫碱(分别含赖氨酸为2g、4g)、2.56g和5.12g赖氨酸盐酸盐作为对照(分别含赖氨酸为2g、4g),以研究对进入绵羊小肠蛋白和游离氨基酸的流量。结果表明,在赖氨酸实际添加水平都为4g/d时,赖氨酸席夫碱使绵羊小肠液中游离Lys、Glu和Tyr的流量分别提高了25.17%、38.35%和33.88%(P<0.05),对其他游离氨基酸流量的影响不明显;添加赖氨酸席夫碱不影响绵羊的粗蛋白采食量、瘤胃微生物蛋白的合成和进入小肠的瘤胃非降解蛋白。
选用4只体重在40kg左右,安装永久性十二指肠瘘管的中国美利奴(新疆型)细毛羊,采用拉丁方设计,分别添加3.42g和6.84g赖氨酸席夫碱(分别含赖氨酸为2g、4g)、2.56g和5.12g赖氨酸盐酸盐作为对照(分别含赖氨酸为2g、4g),以研究对绵羊瘤胃消化代谢的影响。结果显示,添加6.84g赖氨酸席夫碱时绵羊瘤胃液乙酸含量降低了9.44%(P<0.05),随添加量由3.42g增加至6.84g时,丁酸含量增加了10.64%(P<0.05)。试验各组绵羊的平均采食量、瘤胃pH值、NH3-N含量和瘤胃原虫数量分别为1374.21～1419.01g/d、6.28～6.40、16.44～18.31mg/100mL、86.31×10~4～89.92×10~4,各组间差异不显著;对瘤胃内干物质、有机物、粗纤维和纤维素的表观消化率无显著影响。
本文研究表明,添喂6.84g赖氨酸席夫碱(含赖氨酸4g)可显著提高每日到达绵羊小肠游离Lys、Glu和Tyr的流量,同时具有降低瘤胃液中的乙酸含量和总挥发性脂肪酸浓度的作用。
The in vitro antibacterial activity of the Schiff’s base of lysine, the effects of supplementing lysine and Schiff’s base of lysine were studied on flow of protein, liquid free amino acids to the small intestine and rumen digestion and metabolism.
The Schiff’s base of lysine was synthesized by lysine and salicylaldehyde. The minimum inhibiting concentration against E.coli,D. aureds and Salmonella were determined by concentration dilution method. Bactericidal curve was made by three times MIC of Schiff bases of lysine. The results showed that the minimum inhibiting concentration were 786.4, 1228.8 and 983μg/mL, respectively. Antibacterial activity of three times MIC tended to be similar after 4h.
Four male China merino(Xinjiang type) fitted with ruminal and duodenal cannulae were used in a 4×4 Latin square design trial to study the effect of supplemental Schiff’s base of lysine 3.42g/d, 6.84g/d(the content of lysine is 2g and 4g respectively) and L-lysine-HCL 2.56g , 5.12g/d(the content of lysine is 2g and 4g respectively) on the flow of protein and liquid free amino acids to the small intestine. The results indicated that 6.84g/sheep/d Schiff’s base of lysine equal to 4g free lysine increased flow of Lys, Glu and Tyr to the small intestine by 25.17%, 38.35% and 33.88%(P<0.05) respectively. Flows of other free amino acids to the small intestine were not affected by supplementing with Schiff’s base of lysine. Supplementing Schiff’s base of lysine did not affect crude protein intake, microbial protein synthesis and flow of rumen undegradable protein to the small intestine.
Four male China merino (Xinjiang type) fitted with ruminal and duodenal cannulae were used in a 4×4 Latin square design to study the effect of supplementing Schiff’s base of lysine 3.42g/d and 6.84g/d (the content of lysine is 2g and 4g respectively) and L-lysine-HCL 2.56g and 5.12g/d(the content of lysine is 2g and 4g respectively) on rumen digestion and metabolism. The results showed that Schiff’s base of lysine with 4g lysine decreased the concentration of the total volatile fatty acids and acetic acid by 7.24% and 9.44% (P<0.05), respectively. The average concentration of butyric acid increased 10.64% (P<0.05) with supplementing Schiff’s base of lysine from 3.42g/sheep/d to 6.84g/sheep/d. The average dry matter intake , pH, concentration of NH3-N and rumen protozoa in the rumen were 1374.21～1419.01g/d, 6.28～6.40, 16.44 mg/100ml～18.31 mg/100ml and 86.31×10~4～89.91×10~4 respectively, with no significant difference among groups. Rumen apparent digestibility of dry matter, organic matter, cellulose and hemicellulose were not affected by supplementing Schiff’s base of lysine.
It was concluded that 6.84g of Schiff’s base of lysine increased the flow of Lys, Glu and Tyr to the small intestine, and reduced concentrations of acetic acid and total volatile fatty acids in rumen.
采用赖氨酸和水杨醛合成了赖氨酸席夫碱,并运用倍比稀释法进行大肠杆菌、金黄色葡萄球菌和沙门氏杆菌的最小抑菌活性试验,同时根据以上试验测定了在3倍最小抑菌浓度时的抑菌曲线,结果表明,赖氨酸席夫碱对上述试验菌的最小抑菌浓度分别为786.4、1228.8和983μg/mL,3倍最小抑菌曲线显示在4h后对各试验菌的抑菌活性趋于相近。
选用4只体重在40kg左右,安装永久性十二指肠瘘管的中国美利奴(新疆型)细毛羊,采用拉丁方设计,分别添加3.42g和6.84g赖氨酸席夫碱(分别含赖氨酸为2g、4g)、2.56g和5.12g赖氨酸盐酸盐作为对照(分别含赖氨酸为2g、4g),以研究对进入绵羊小肠蛋白和游离氨基酸的流量。结果表明,在赖氨酸实际添加水平都为4g/d时,赖氨酸席夫碱使绵羊小肠液中游离Lys、Glu和Tyr的流量分别提高了25.17%、38.35%和33.88%(P<0.05),对其他游离氨基酸流量的影响不明显;添加赖氨酸席夫碱不影响绵羊的粗蛋白采食量、瘤胃微生物蛋白的合成和进入小肠的瘤胃非降解蛋白。
选用4只体重在40kg左右,安装永久性十二指肠瘘管的中国美利奴(新疆型)细毛羊,采用拉丁方设计,分别添加3.42g和6.84g赖氨酸席夫碱(分别含赖氨酸为2g、4g)、2.56g和5.12g赖氨酸盐酸盐作为对照(分别含赖氨酸为2g、4g),以研究对绵羊瘤胃消化代谢的影响。结果显示,添加6.84g赖氨酸席夫碱时绵羊瘤胃液乙酸含量降低了9.44%(P<0.05),随添加量由3.42g增加至6.84g时,丁酸含量增加了10.64%(P<0.05)。试验各组绵羊的平均采食量、瘤胃pH值、NH3-N含量和瘤胃原虫数量分别为1374.21～1419.01g/d、6.28～6.40、16.44～18.31mg/100mL、86.31×10~4～89.92×10~4,各组间差异不显著;对瘤胃内干物质、有机物、粗纤维和纤维素的表观消化率无显著影响。
本文研究表明,添喂6.84g赖氨酸席夫碱(含赖氨酸4g)可显著提高每日到达绵羊小肠游离Lys、Glu和Tyr的流量,同时具有降低瘤胃液中的乙酸含量和总挥发性脂肪酸浓度的作用。
The in vitro antibacterial activity of the Schiff’s base of lysine, the effects of supplementing lysine and Schiff’s base of lysine were studied on flow of protein, liquid free amino acids to the small intestine and rumen digestion and metabolism.
The Schiff’s base of lysine was synthesized by lysine and salicylaldehyde. The minimum inhibiting concentration against E.coli,D. aureds and Salmonella were determined by concentration dilution method. Bactericidal curve was made by three times MIC of Schiff bases of lysine. The results showed that the minimum inhibiting concentration were 786.4, 1228.8 and 983μg/mL, respectively. Antibacterial activity of three times MIC tended to be similar after 4h.
Four male China merino(Xinjiang type) fitted with ruminal and duodenal cannulae were used in a 4×4 Latin square design trial to study the effect of supplemental Schiff’s base of lysine 3.42g/d, 6.84g/d(the content of lysine is 2g and 4g respectively) and L-lysine-HCL 2.56g , 5.12g/d(the content of lysine is 2g and 4g respectively) on the flow of protein and liquid free amino acids to the small intestine. The results indicated that 6.84g/sheep/d Schiff’s base of lysine equal to 4g free lysine increased flow of Lys, Glu and Tyr to the small intestine by 25.17%, 38.35% and 33.88%(P<0.05) respectively. Flows of other free amino acids to the small intestine were not affected by supplementing with Schiff’s base of lysine. Supplementing Schiff’s base of lysine did not affect crude protein intake, microbial protein synthesis and flow of rumen undegradable protein to the small intestine.
Four male China merino (Xinjiang type) fitted with ruminal and duodenal cannulae were used in a 4×4 Latin square design to study the effect of supplementing Schiff’s base of lysine 3.42g/d and 6.84g/d (the content of lysine is 2g and 4g respectively) and L-lysine-HCL 2.56g and 5.12g/d(the content of lysine is 2g and 4g respectively) on rumen digestion and metabolism. The results showed that Schiff’s base of lysine with 4g lysine decreased the concentration of the total volatile fatty acids and acetic acid by 7.24% and 9.44% (P<0.05), respectively. The average concentration of butyric acid increased 10.64% (P<0.05) with supplementing Schiff’s base of lysine from 3.42g/sheep/d to 6.84g/sheep/d. The average dry matter intake , pH, concentration of NH3-N and rumen protozoa in the rumen were 1374.21～1419.01g/d, 6.28～6.40, 16.44 mg/100ml～18.31 mg/100ml and 86.31×10~4～89.91×10~4 respectively, with no significant difference among groups. Rumen apparent digestibility of dry matter, organic matter, cellulose and hemicellulose were not affected by supplementing Schiff’s base of lysine.
It was concluded that 6.84g of Schiff’s base of lysine increased the flow of Lys, Glu and Tyr to the small intestine, and reduced concentrations of acetic acid and total volatile fatty acids in rumen.
引文
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