无机源性与生物源性骨组织替代材料成骨效果的动物实验研究
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摘要
目的：通过动物实验,评价无机源性与生物源性骨组织替代材料,在骨缺损再生修复中引导骨组织再生的能力和特点及其差异。
方法：对18只兔颅骨骨缺损区采用无机源性与生物源性骨粉填充并用胶原生物膜覆盖通过X线检查、骨组织切片苏木素-伊红染色(HE染色)和Masson三色染色图像分析观察骨缺损区不同实验程期(12、16、24周)的组织修复状况。
结果：无机源性与生物源性骨替代材料在兔颅骨的骨缺损修复中,具有良好的生物相容性和引导骨组织再生性和操作性。
结论：无机源性与生物源性骨粉均可起到引导新骨再生,完全修复骨缺损的作用。但生物源性骨组织替代材料的成骨性能优于无机源性骨组织替代材料的成骨性能。
Objective:The study which was carried through animal experiment evaluated the ability, feature and differences in guiding new bony tissue regeneration of two bony substitutional material inorganic-derived and bio-derived bone in their reparation of bony defects.
Methods:Filled the bony defect areas in 18 rabbits' calvarium with inorganic-derived or bio-derived bone meal and then cover with collagen membrane. The repairing situation is observed and analysed by X-ray, HE and Masson's trichrome staining on the bone tissue slices at 12,16 and 24 weeks after the operation.
Results:Both of inorganic-derived and bio-derived bone meal have biocompatibility, the ability to guid bony tissue regeneration and the possibility to operate in the process of repairing rabbits' calvarium defects.
Conclusion:Both of inorganic-derived and bio-derived bone meal can both guid bony tissue regeneration and completely cover the calvarium defects. And, the bio-derived is better.
方法：对18只兔颅骨骨缺损区采用无机源性与生物源性骨粉填充并用胶原生物膜覆盖通过X线检查、骨组织切片苏木素-伊红染色(HE染色)和Masson三色染色图像分析观察骨缺损区不同实验程期(12、16、24周)的组织修复状况。
结果：无机源性与生物源性骨替代材料在兔颅骨的骨缺损修复中,具有良好的生物相容性和引导骨组织再生性和操作性。
结论：无机源性与生物源性骨粉均可起到引导新骨再生,完全修复骨缺损的作用。但生物源性骨组织替代材料的成骨性能优于无机源性骨组织替代材料的成骨性能。
Objective:The study which was carried through animal experiment evaluated the ability, feature and differences in guiding new bony tissue regeneration of two bony substitutional material inorganic-derived and bio-derived bone in their reparation of bony defects.
Methods:Filled the bony defect areas in 18 rabbits' calvarium with inorganic-derived or bio-derived bone meal and then cover with collagen membrane. The repairing situation is observed and analysed by X-ray, HE and Masson's trichrome staining on the bone tissue slices at 12,16 and 24 weeks after the operation.
Results:Both of inorganic-derived and bio-derived bone meal have biocompatibility, the ability to guid bony tissue regeneration and the possibility to operate in the process of repairing rabbits' calvarium defects.
Conclusion:Both of inorganic-derived and bio-derived bone meal can both guid bony tissue regeneration and completely cover the calvarium defects. And, the bio-derived is better.
引文
1 Stricker A, Voss PJ, Gutwald R, et al. Maxillary sinus floor augmention with autogenous bone grafts to enable placement of SLA-surfaced implants:preliminary results after 15-40 months. Clin Oral Implants Res,2003,14(2):207-212
2 Scarano A, Degidi M, Iezzi G, et al. Maxillary sinus augmentation with diferent biomaterials:a comparative histologic and histomorphometric study in man. Implant Dent,2006,15(2):197-207
3 Stellingsma K, Bouma J, Stegenga B, et al. Satisfaction and psychosocial aspects of patients with an extremely resorbed mandible treated with implant-retained overdentures. A prospective, comparative study. Clin Oral Implants Res,2003; 14(2):166-172
4 Haddad, Albert J, Peel, Sean A. F. Clokie, Cameron M. L. Sandor, George K. B.;Closure of Rabbit Calvarial Critical-Sized Defects Using Protective Composite Allogeneic and Alloplastic Bone Substitutes; Journal of Craniofacial Surgery; 17(5),2006.9; 926-934
5 Duda M, Pajak J. The issue of bioresorption of the Bio-Oss xenogeneic bone substitute in bone defects. Ann Univ Mariae Curie Sklodowska[Med].2004,59(1):269-77.
6刘超,周正国,孔庆海.Bio-Oss和邻近自体碎骨骨移植在牙种植术中的应用[J].徐州医学院学报,2005,(4)：333-335.
7 Kay SA, Lynch LW, Marxer M, et al. Practic Periodont Aesthetic Dentistry,1997,9(2):185-194
8 John PS, Teffery OH. The Critical Size Defect as an Experimental Model for Craniomandibulofacial Nonunions [J]. Clinical orvlopaedics and Related Research,1986, 205 (1):299-308
9陈刚、赵士杰.用于评价骨修复材料的临界性骨缺损[J].国外医学口腔医学分册,2001,3(28)：71-73
10 Nociti Jr FH, Machado MA, Stefani CM, et al. Absorbable versus nonabsorbable membranes and bone grafts in the treatment of ligature-included peri-implantitis defects in dogs. Part I. Aclinical investigation[J]. Clin Oral Imp 1 Res,2001,12 (1): 115-120
11 Hockers T, AbensurD, Valentini P, et al. The combined use of bioresorbable membranes and xenogragts or autografts in the treatment of bone defects around imp lants:a study in beagle dogs[J]. Clin Oral Impl Res,1999, 10 (1):487-498
12 Hammerle CHF, lang NP. Single stage surgery combining transmu-cosal implant placement with guide bone regeneration and bioresorbable materials[J]. Clin Oral Imp 1 Res,2001,12 (1):9-18
13龚飞飞,周东生,李容新,等.天机活性元素组织工程支架重建修复山羊颌骨缺损的扫描电镜观察[J].口腔医学研究,2008,24(1)：12-14
14 Chen Y, Chen ZX, Xu H, Wang WZ. Modern research on mechanism of bone fracture healing. Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu 2008;12(50):9951-9956
15 Fang L, Wang W, Shen G, et al. Bone tissue engineering scaffolds. sheng wu yi xue Gong cheng xue za zhi,2003,20 (1):148-152
16 Sundarammoorthi R, Shakespeare WC, Keenan TP, et al. Bone-targeted Src kinase inhibitors:novelk pyrrolo-andkpyrazolo-pyrimidine analogues. Bioorg Med Chem Lett,2003,13 (18):3063-3066
17.Steinberg EL, Golomb D, Salama R, et al. Radial bead and neck fractures in childen[J]. Pediatrorthop,1998,8 (1): 35-40
18 PeetzM. Characterization Of Xenogeneic BoneMaterial. In Boyne PJ, eds. Osseous Recontruction of theMaxilla and theMandible.1st ed [M]. Quintessence Publishing:Gemany, 1997,87-100
19耿威,宿玉成,徐刚,等.无机牛骨结合可吸收性胶原膜修复种植牙骨缺损的定量分析[J].口腔医学研究,2003,19(2)：280-283
20 SpectorM. Anorganic bovine bone and ceramic analogs of bone mineral as imp lants to facilitate bone regeneration[J]. Clin Plast Surg,1994,21:437-444
21 Benhayoune Hdalot E, Laquerriere P, et al. Integration of dense HA rods into cortical bone. Biomaterials,2000,21 (3): 235-242
22 So K, Fujibayashi S, Neo M, et al. Accelerated degradation and improved bone-bonding ability of hydroxyapatite ceramics by the addition of glass. Biomatedals,2006, 27(27):4738-4744
23 Chen ZF, Darvell BW, Leung VW. Hydroxyapatite solubility in simple inorganic solutions. Arch Oral Biol,2004,49(5): 359-367
1 Stricker A, Voss PJ, Gutwald R, et al. Maxillary sinus floor augmention with autogenous bone grafts to enable placement of SLA-surfaced implants:preliminary results after 15-40 months. Clin Oral Implants Res,2003,14(2):207-212
2 Scarano A, Degidi M, Iezzi G, et al. Maxillary sinus augmentation with diferent biomaterials:a comparative histologic and histomorphometric study in man. Implant Dent,2006,15(2):197-207
3 Stellingsma K, Bouma J, Stegenga B, et al. Satisfaction and psychosocial aspects of patients with an extremely resorbed mandible treated with implant-retained overdentures. A prospective, comparative study. Clin Oral Implants Res,2003; 14(2):166-172
4 Haddad, Albert J, Peel, Sean A. F. Clokie, Cameron M. L. Sandor, George K. B.;Closure of Rabbit Calvarial Critical-Sized Defects Using Protective Composite Allogeneic and Alloplastic Bone Substitutes;Journal of Craniofacial Surgery; 17(5),2006.9; 926-934
5马振国,周树夏,刘宝林.不同脱矿时间皮质骨强度矿物质含量及骨诱导能力的关系[J].中华骨科杂志,1993,(2)：117.
6贺文,齐秋长,徐梅,等.大段异体骨支撑复合髂骨移植修复肱骨良性骨缺损[J].解剖与临床,2002,(1P2)：46-47.
7 Muramatsu K, Bishop AT, Sunagawa T, Fate of donor cells in vascularized bone grafts:identification of systemic chimerism by the polymerase chain reaction. Plast Reconstr Surg.2003 Feb,111(2):763-72; discussion 773-4.
8 Minichetti JC, D'Amore JC, Hong AY. Three-year analysis of Tapered Screw-Vent implants placed into extraction sockets grafted with mineralized bone allograft. J Oral Implantol, 2005,31(6):283-293
9张志鹏,闫冰,闰景龙.同种异体微小颗粒骨P磷酸钙骨水泥复合物修复骨缺损的实验研究[J].中国康复医学杂志,2005,(7)：514-516.
10 Duda M, Pajak J. The issue of bioresorption of the Bio-Oss xenogeneic bone substitute in bone defects. Ann Univ Mariae Curie Sklodowska[Med].2004,59(1):269-77.
11刘超,周正国,孔庆海.Bio-Oss和邻近自体碎骨骨移植在牙种植术中的应用[J].徐州医学院学报,2005,(4)：333-335.
12 Kay SA, Lynch LW, Marxer M, et al. Practic Periodont Aesthetic Dentistry,1997,9(2):185-194
13 PeetzM. Characterization Of Xenogeneic BoneMaterial. In Boyne PJ, eds. Osseous Recontruction of theMaxilla and theMandible.1st ed [M]. Quintessence Publishing:Gemany, 1997,87-100
14耿威,宿玉成,徐刚,等.无机牛骨结合可吸收性胶原膜修复种植牙骨缺损的定量分析[J].口腔医学研究,2003,19(2)：280-283
15 SpectorM. Anorganic bovine bone and ceramic analogs of bone mineral as imp lants to facilitate bone regeneration [J]. Clin Plast Surg,1994,21:437-444
16 Guillemin G, Patat JL. The use of coral as a bone graft substitute. J Biomed Mater Res,1987,21 (5):557
17 Louisia S, Stromboni M,Meunier A, et al. Coral grafting supplemented with bone marrow. J Bone Joint Surg Br,1999,81 (4):719
18 Sous M,Bareille R, Rouais F, et al. Cellular biocopatibility and resistance compression of macroporous beta-tricalcium phosphate ceramics [J]. Biomaterials,1998,19 (23);2147-53.
19 Ganeles J,Listgatrten MA,Evian CI. Ultrastructure of durapate periodontal tissue interfice in human intrabony defects[J]. J Periodontal,1986,57:133-134.
20 Piecuch J F. Exyraskeletal implantion of porous hydroxyapatite ceramic[J]. J Dent Res,1982,61:1458--60
21 Turnbull RS, Amler MH. Histological comparison of hydroxyapatite and betatricalcium phosphate implants in the rat parietal bone[J]. J Dent Res,1985,64:218-219.
22 Szpalski M, Gunzburg R. Application of calcium phosphate-based cancellous bone void fillers on trauma surgery. Orthopedics,2002,25(5 Suppl):597-600.
23 Wiltfang J, Merten HA, Schlegel KA, Degradation characteristics of alpha and beta tri-calcium-phosphate (TCP) in minipigs. J Biomed Mater Res. 2002,63(2):115-121.
24 Kishimoto M, Kanemaru S, Yamashita M, et al. Cranial boneregeneration using a composite scaffold of Beta-tricalcium phosphate, collagen, and autologous bone fragments. Laryngoscope 2006;116(2):212-216
25 Chazono M, Tanaka T, Komaki H, et al. Bone formation and bioresorption'after implantation of injectable beta-tricalcium phosphate granules-hyaluronate complex in rabbit bone defects. J Biomed Mater Res 200; 70 (4):542-549
26 Ogose A, Hotta T, Kawashima H, et al. Comparison of hydroxyapatite and beta tricalcium phosphate as bone substitutes after excision of bone tumors. J Biomed Mater Res 2005;72 (1):94-101
27 Zerbo IR, Zijderveld SA, de Boer A, et al. Histomorphometry of human sinus floor augm entation using a porous β-tricalcium phosphate:a prospective study. Clin Oral Implants Res,2004,15(6):724-732
28 Zerbo IR, Bronckem AL, de Lange G, et al. Localisation of osteogenic and osteoclastic cells in po rous beta-tricalcium phosphate particles used for human maxillary sinus flor elevation. Biomaterials,2005,26(12): 1445-1451
29 Suba Z, Takacs D, Matusovits D, et al. Maxillary sinus floor grafting with beta-tricalcium phosphate in humans:density and microarchitecture of the newly formed bone. Clin Oral Implants Res,2006,17(1):102-108
2 Scarano A, Degidi M, Iezzi G, et al. Maxillary sinus augmentation with diferent biomaterials:a comparative histologic and histomorphometric study in man. Implant Dent,2006,15(2):197-207
3 Stellingsma K, Bouma J, Stegenga B, et al. Satisfaction and psychosocial aspects of patients with an extremely resorbed mandible treated with implant-retained overdentures. A prospective, comparative study. Clin Oral Implants Res,2003; 14(2):166-172
4 Haddad, Albert J, Peel, Sean A. F. Clokie, Cameron M. L. Sandor, George K. B.;Closure of Rabbit Calvarial Critical-Sized Defects Using Protective Composite Allogeneic and Alloplastic Bone Substitutes; Journal of Craniofacial Surgery; 17(5),2006.9; 926-934
5 Duda M, Pajak J. The issue of bioresorption of the Bio-Oss xenogeneic bone substitute in bone defects. Ann Univ Mariae Curie Sklodowska[Med].2004,59(1):269-77.
6刘超,周正国,孔庆海.Bio-Oss和邻近自体碎骨骨移植在牙种植术中的应用[J].徐州医学院学报,2005,(4)：333-335.
7 Kay SA, Lynch LW, Marxer M, et al. Practic Periodont Aesthetic Dentistry,1997,9(2):185-194
8 John PS, Teffery OH. The Critical Size Defect as an Experimental Model for Craniomandibulofacial Nonunions [J]. Clinical orvlopaedics and Related Research,1986, 205 (1):299-308
9陈刚、赵士杰.用于评价骨修复材料的临界性骨缺损[J].国外医学口腔医学分册,2001,3(28)：71-73
10 Nociti Jr FH, Machado MA, Stefani CM, et al. Absorbable versus nonabsorbable membranes and bone grafts in the treatment of ligature-included peri-implantitis defects in dogs. Part I. Aclinical investigation[J]. Clin Oral Imp 1 Res,2001,12 (1): 115-120
11 Hockers T, AbensurD, Valentini P, et al. The combined use of bioresorbable membranes and xenogragts or autografts in the treatment of bone defects around imp lants:a study in beagle dogs[J]. Clin Oral Impl Res,1999, 10 (1):487-498
12 Hammerle CHF, lang NP. Single stage surgery combining transmu-cosal implant placement with guide bone regeneration and bioresorbable materials[J]. Clin Oral Imp 1 Res,2001,12 (1):9-18
13龚飞飞,周东生,李容新,等.天机活性元素组织工程支架重建修复山羊颌骨缺损的扫描电镜观察[J].口腔医学研究,2008,24(1)：12-14
14 Chen Y, Chen ZX, Xu H, Wang WZ. Modern research on mechanism of bone fracture healing. Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu 2008;12(50):9951-9956
15 Fang L, Wang W, Shen G, et al. Bone tissue engineering scaffolds. sheng wu yi xue Gong cheng xue za zhi,2003,20 (1):148-152
16 Sundarammoorthi R, Shakespeare WC, Keenan TP, et al. Bone-targeted Src kinase inhibitors:novelk pyrrolo-andkpyrazolo-pyrimidine analogues. Bioorg Med Chem Lett,2003,13 (18):3063-3066
17.Steinberg EL, Golomb D, Salama R, et al. Radial bead and neck fractures in childen[J]. Pediatrorthop,1998,8 (1): 35-40
18 PeetzM. Characterization Of Xenogeneic BoneMaterial. In Boyne PJ, eds. Osseous Recontruction of theMaxilla and theMandible.1st ed [M]. Quintessence Publishing:Gemany, 1997,87-100
19耿威,宿玉成,徐刚,等.无机牛骨结合可吸收性胶原膜修复种植牙骨缺损的定量分析[J].口腔医学研究,2003,19(2)：280-283
20 SpectorM. Anorganic bovine bone and ceramic analogs of bone mineral as imp lants to facilitate bone regeneration[J]. Clin Plast Surg,1994,21:437-444
21 Benhayoune Hdalot E, Laquerriere P, et al. Integration of dense HA rods into cortical bone. Biomaterials,2000,21 (3): 235-242
22 So K, Fujibayashi S, Neo M, et al. Accelerated degradation and improved bone-bonding ability of hydroxyapatite ceramics by the addition of glass. Biomatedals,2006, 27(27):4738-4744
23 Chen ZF, Darvell BW, Leung VW. Hydroxyapatite solubility in simple inorganic solutions. Arch Oral Biol,2004,49(5): 359-367
1 Stricker A, Voss PJ, Gutwald R, et al. Maxillary sinus floor augmention with autogenous bone grafts to enable placement of SLA-surfaced implants:preliminary results after 15-40 months. Clin Oral Implants Res,2003,14(2):207-212
2 Scarano A, Degidi M, Iezzi G, et al. Maxillary sinus augmentation with diferent biomaterials:a comparative histologic and histomorphometric study in man. Implant Dent,2006,15(2):197-207
3 Stellingsma K, Bouma J, Stegenga B, et al. Satisfaction and psychosocial aspects of patients with an extremely resorbed mandible treated with implant-retained overdentures. A prospective, comparative study. Clin Oral Implants Res,2003; 14(2):166-172
4 Haddad, Albert J, Peel, Sean A. F. Clokie, Cameron M. L. Sandor, George K. B.;Closure of Rabbit Calvarial Critical-Sized Defects Using Protective Composite Allogeneic and Alloplastic Bone Substitutes;Journal of Craniofacial Surgery; 17(5),2006.9; 926-934
5马振国,周树夏,刘宝林.不同脱矿时间皮质骨强度矿物质含量及骨诱导能力的关系[J].中华骨科杂志,1993,(2)：117.
6贺文,齐秋长,徐梅,等.大段异体骨支撑复合髂骨移植修复肱骨良性骨缺损[J].解剖与临床,2002,(1P2)：46-47.
7 Muramatsu K, Bishop AT, Sunagawa T, Fate of donor cells in vascularized bone grafts:identification of systemic chimerism by the polymerase chain reaction. Plast Reconstr Surg.2003 Feb,111(2):763-72; discussion 773-4.
8 Minichetti JC, D'Amore JC, Hong AY. Three-year analysis of Tapered Screw-Vent implants placed into extraction sockets grafted with mineralized bone allograft. J Oral Implantol, 2005,31(6):283-293
9张志鹏,闫冰,闰景龙.同种异体微小颗粒骨P磷酸钙骨水泥复合物修复骨缺损的实验研究[J].中国康复医学杂志,2005,(7)：514-516.
10 Duda M, Pajak J. The issue of bioresorption of the Bio-Oss xenogeneic bone substitute in bone defects. Ann Univ Mariae Curie Sklodowska[Med].2004,59(1):269-77.
11刘超,周正国,孔庆海.Bio-Oss和邻近自体碎骨骨移植在牙种植术中的应用[J].徐州医学院学报,2005,(4)：333-335.
12 Kay SA, Lynch LW, Marxer M, et al. Practic Periodont Aesthetic Dentistry,1997,9(2):185-194
13 PeetzM. Characterization Of Xenogeneic BoneMaterial. In Boyne PJ, eds. Osseous Recontruction of theMaxilla and theMandible.1st ed [M]. Quintessence Publishing:Gemany, 1997,87-100
14耿威,宿玉成,徐刚,等.无机牛骨结合可吸收性胶原膜修复种植牙骨缺损的定量分析[J].口腔医学研究,2003,19(2)：280-283
15 SpectorM. Anorganic bovine bone and ceramic analogs of bone mineral as imp lants to facilitate bone regeneration [J]. Clin Plast Surg,1994,21:437-444
16 Guillemin G, Patat JL. The use of coral as a bone graft substitute. J Biomed Mater Res,1987,21 (5):557
17 Louisia S, Stromboni M,Meunier A, et al. Coral grafting supplemented with bone marrow. J Bone Joint Surg Br,1999,81 (4):719
18 Sous M,Bareille R, Rouais F, et al. Cellular biocopatibility and resistance compression of macroporous beta-tricalcium phosphate ceramics [J]. Biomaterials,1998,19 (23);2147-53.
19 Ganeles J,Listgatrten MA,Evian CI. Ultrastructure of durapate periodontal tissue interfice in human intrabony defects[J]. J Periodontal,1986,57:133-134.
20 Piecuch J F. Exyraskeletal implantion of porous hydroxyapatite ceramic[J]. J Dent Res,1982,61:1458--60
21 Turnbull RS, Amler MH. Histological comparison of hydroxyapatite and betatricalcium phosphate implants in the rat parietal bone[J]. J Dent Res,1985,64:218-219.
22 Szpalski M, Gunzburg R. Application of calcium phosphate-based cancellous bone void fillers on trauma surgery. Orthopedics,2002,25(5 Suppl):597-600.
23 Wiltfang J, Merten HA, Schlegel KA, Degradation characteristics of alpha and beta tri-calcium-phosphate (TCP) in minipigs. J Biomed Mater Res. 2002,63(2):115-121.
24 Kishimoto M, Kanemaru S, Yamashita M, et al. Cranial boneregeneration using a composite scaffold of Beta-tricalcium phosphate, collagen, and autologous bone fragments. Laryngoscope 2006;116(2):212-216
25 Chazono M, Tanaka T, Komaki H, et al. Bone formation and bioresorption'after implantation of injectable beta-tricalcium phosphate granules-hyaluronate complex in rabbit bone defects. J Biomed Mater Res 200; 70 (4):542-549
26 Ogose A, Hotta T, Kawashima H, et al. Comparison of hydroxyapatite and beta tricalcium phosphate as bone substitutes after excision of bone tumors. J Biomed Mater Res 2005;72 (1):94-101
27 Zerbo IR, Zijderveld SA, de Boer A, et al. Histomorphometry of human sinus floor augm entation using a porous β-tricalcium phosphate:a prospective study. Clin Oral Implants Res,2004,15(6):724-732
28 Zerbo IR, Bronckem AL, de Lange G, et al. Localisation of osteogenic and osteoclastic cells in po rous beta-tricalcium phosphate particles used for human maxillary sinus flor elevation. Biomaterials,2005,26(12): 1445-1451
29 Suba Z, Takacs D, Matusovits D, et al. Maxillary sinus floor grafting with beta-tricalcium phosphate in humans:density and microarchitecture of the newly formed bone. Clin Oral Implants Res,2006,17(1):102-108
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