纳米银/海藻酸钠复合薄膜的制备及其性能研究
摘要
纳米银优良的抗菌性能和潜在的选择性抑制细胞增生性能引起了人们的关注,希望除现有的体外应用外,还能够用于人体植入体如血管支架等,预防植入后局部细胞过度增生的问题。为了使纳米银粒子能应用于各种不同的植入材料表面,并且避免过多的自由粒子与人体接触后带来的安全隐患,本研究拟采用海藻酸钠作为载体,将纳米银固化在其中,制备出纳米银／海藻酸钠复合薄膜,通过缓控释作用将纳米银逐步释放出来,在局部维持一定的作用浓度,达到持续抑制细胞增生的同时,减轻纳米银的团聚和迁移带来的危害。
本文采用机械混合匀胶凝胶法制备出均匀完整,与玻片基底间具有一定结合强度的纳米银／海藻酸钠复合薄膜,采用x射线衍射(XRD)、X射线能谱分析(EDS)、扫描电子显微镜观察(SEM)、原子力显微镜观察(AFM)、紫外分光光度法测量、电化学法Ⅳ曲线测量等手段,对薄膜中纳米银的成份与分布,薄膜的表面形貌、电学性能、缓释性能等进行了研究；同时采用MTT、LDH法研究了复合薄膜对体外细胞培养增殖性能的影响,对纳米银／海藻酸钠复合薄膜的缓释机制以及对细胞增殖产生影响的原因进行了分析和讨论。
实验结果表明：采用凝胶匀胶法,利用含有不同浓度纳米银的2.5%的海藻酸钠溶胶,匀胶机转速为4000 r/min条件下,可在玻璃基底上制备出厚度约为1μm完整均匀的纳米银／海藻酸钠复合薄膜。复合薄膜具有欧姆特性,其电阻值随纳米银掺杂量的增加而增加,符合麦克斯韦的有效介质模型。薄膜表面不平整,均布着直径约为1μm左右的凹坑,纳米银以这些凹坑为分布形态,以物理混合的方式总体均匀地存在于薄膜中。复合薄膜以侵蚀作为主要的缓释机制,银的缓释量与掺入银的浓度、缓释液体环境有关。纳米银的掺杂量越大,在相同时间内释放出来的纳米银越多,其中部分银是包含在海藻酸钠碎屑中的。在类体液中薄膜的缓释速率最小,在无血清的培养基中薄膜的缓释速率次之,在有血清培养清中薄膜的缓释速率最大。纳米银／海藻酸钠复合薄膜对细胞具有显著的抑制作用,其作用主要来自于被释放出来的银粒子与细胞间的交互作用,以及侵蚀碎屑给细胞生长带来的影响。
The excellent antibacterial property of nano-Ag, as well as its selective-restrain effect on cell proliferation caused interests of applying it on human implants besides present application of external antimicrobial. For example, apply it on human vascular stent to avoid restenosis. In the sake of apply nano-Ag on different kinds of implant surface, and reduce the security problem caused by the use of nano particles, sodium alginate (AGS) was considered to be used as the carrier of nano-Ag in this study. Via controlling the releasing rate of nano-Ag from the film, the local concentration of Ag can be continuously maintained to a certain level, which is benefit to reducing the harm of aggregation and immigration of nano-Ag while inhibiting the cell proliferation.
In this paper, nano-Ag/AGS composite film is prepared on glass substrate by spin coating method, using the nano-Ag and 2.5 w.t.% AGS mixture as the sol. By using X-ray diffraction (XRD), X-ray energy spectra (EDS), Scan electronic microscopy (SEM), Atomic force microscopy (AFM), UV-Spectrophotometry, andⅣmeasurement method, the distribution of nano-Ag in the film, the surface morphology of the film, the electronic property and releasing property of the films are studied. Meanwhile, the cell proliferation behavior on the films was study by MTT assay, and the acute cytotoxicity of the films was studied by LDH as well. The releasing mechanism and the inhibition effect of the film are discussed in detail..
The results show that:The nano-Ag/AGS composite films prepared here are uniform, with the thickness of 1μm. The films have Ohmic contact property, whose resistance is increasing with the increase of Ag doping concentration, which matches the model of Maxwell's theory of effective media. The surface morphology is rough with concavities. The distribution of Ag is generally uniform and follows the shape of the concavities. The releasing mechanism of the films is erosion, and the releasing rate is related to the concentration of nano-Ag and the solution. The released nano-Ag partly exists in the chips peeled from film, and its total amount is increasing with its doping concentration. The releasing rate of Ag is different among simulate body fluid (SBF), cell culture medium without bovine albumin, and medium with bovine albumin, which is low in SFB and high in medium with bovine albumin. Nano-Ag/AGS films have significant effect on the restrain of cell proliferation, and the reason can be attributed to the interaction between the released nano-Ag particles, and the influence of the peeled chips.
本文采用机械混合匀胶凝胶法制备出均匀完整,与玻片基底间具有一定结合强度的纳米银／海藻酸钠复合薄膜,采用x射线衍射(XRD)、X射线能谱分析(EDS)、扫描电子显微镜观察(SEM)、原子力显微镜观察(AFM)、紫外分光光度法测量、电化学法Ⅳ曲线测量等手段,对薄膜中纳米银的成份与分布,薄膜的表面形貌、电学性能、缓释性能等进行了研究；同时采用MTT、LDH法研究了复合薄膜对体外细胞培养增殖性能的影响,对纳米银／海藻酸钠复合薄膜的缓释机制以及对细胞增殖产生影响的原因进行了分析和讨论。
实验结果表明：采用凝胶匀胶法,利用含有不同浓度纳米银的2.5%的海藻酸钠溶胶,匀胶机转速为4000 r/min条件下,可在玻璃基底上制备出厚度约为1μm完整均匀的纳米银／海藻酸钠复合薄膜。复合薄膜具有欧姆特性,其电阻值随纳米银掺杂量的增加而增加,符合麦克斯韦的有效介质模型。薄膜表面不平整,均布着直径约为1μm左右的凹坑,纳米银以这些凹坑为分布形态,以物理混合的方式总体均匀地存在于薄膜中。复合薄膜以侵蚀作为主要的缓释机制,银的缓释量与掺入银的浓度、缓释液体环境有关。纳米银的掺杂量越大,在相同时间内释放出来的纳米银越多,其中部分银是包含在海藻酸钠碎屑中的。在类体液中薄膜的缓释速率最小,在无血清的培养基中薄膜的缓释速率次之,在有血清培养清中薄膜的缓释速率最大。纳米银／海藻酸钠复合薄膜对细胞具有显著的抑制作用,其作用主要来自于被释放出来的银粒子与细胞间的交互作用,以及侵蚀碎屑给细胞生长带来的影响。
The excellent antibacterial property of nano-Ag, as well as its selective-restrain effect on cell proliferation caused interests of applying it on human implants besides present application of external antimicrobial. For example, apply it on human vascular stent to avoid restenosis. In the sake of apply nano-Ag on different kinds of implant surface, and reduce the security problem caused by the use of nano particles, sodium alginate (AGS) was considered to be used as the carrier of nano-Ag in this study. Via controlling the releasing rate of nano-Ag from the film, the local concentration of Ag can be continuously maintained to a certain level, which is benefit to reducing the harm of aggregation and immigration of nano-Ag while inhibiting the cell proliferation.
In this paper, nano-Ag/AGS composite film is prepared on glass substrate by spin coating method, using the nano-Ag and 2.5 w.t.% AGS mixture as the sol. By using X-ray diffraction (XRD), X-ray energy spectra (EDS), Scan electronic microscopy (SEM), Atomic force microscopy (AFM), UV-Spectrophotometry, andⅣmeasurement method, the distribution of nano-Ag in the film, the surface morphology of the film, the electronic property and releasing property of the films are studied. Meanwhile, the cell proliferation behavior on the films was study by MTT assay, and the acute cytotoxicity of the films was studied by LDH as well. The releasing mechanism and the inhibition effect of the film are discussed in detail..
The results show that:The nano-Ag/AGS composite films prepared here are uniform, with the thickness of 1μm. The films have Ohmic contact property, whose resistance is increasing with the increase of Ag doping concentration, which matches the model of Maxwell's theory of effective media. The surface morphology is rough with concavities. The distribution of Ag is generally uniform and follows the shape of the concavities. The releasing mechanism of the films is erosion, and the releasing rate is related to the concentration of nano-Ag and the solution. The released nano-Ag partly exists in the chips peeled from film, and its total amount is increasing with its doping concentration. The releasing rate of Ag is different among simulate body fluid (SBF), cell culture medium without bovine albumin, and medium with bovine albumin, which is low in SFB and high in medium with bovine albumin. Nano-Ag/AGS films have significant effect on the restrain of cell proliferation, and the reason can be attributed to the interaction between the released nano-Ag particles, and the influence of the peeled chips.
引文
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