猪伪狂犬病病毒gC基因的克隆及生物信息学分析
摘要
本实验提取了四川农业大学动物生物技术中心保存的Fa株、疫苗株783株、Bartha株和SA215株,四川野毒株SN株、SS株、SQ株、SL株和SCZ株,以及由韶关学院英东生物工程学院娄高明教授惠赠的北京BJ株、广东DG株、湖北HS株共计12株猪伪狂犬病病毒株的DNA并作为模板,应用PCR技术分别扩增得到了12条伪狂犬病毒完整的gC基因的片段。回收PCR产物,成功连接转化到感受态细胞大肠杆菌DH5α中,并通过菌落PCR和酶切鉴定正确后,送生物公司进行测序。
将所得12条序列连同GenBank中登录的6条gC基因全序列共18条基因序列,使用生物软件对gC基因核苷酸序列的同源性、突变区域的定位、遗传进化关系、酶切位点的差异、密码子偏爱性、氨基酸序列的同源性、蛋白质亲水性、抗原表位分析、二级结构和三级结构预测等生物信息学内容进行分析。结果表明:PRV-gC基因的开放阅读框的核苷酸长度在1437bp～1464bp之间,氨基酸长度在478～487个之间,核苷酸同源性在94.9%～100%之间,氨基酸的同源性在90.2%～100%之间;在核酸175～222位存在高变缺失区,其中共有8个碱基突变和21个碱基的插入或缺失;在遗传进化关系上,四川地区流行的毒株与日本、欧美毒株有较近的亲缘关系,而我国其它地区的毒株间的遗传相关性较高,但与日本、欧美洲毒株亲缘关系较远;在18条PRV-gC基因核苷酸序列中均没有发现HindⅢ酶切位点,同时Fa株、DG株、SA215株、BJ株、Min A株和Ea株也没有BamHⅠ位点,Yamagata S-81株则没有SalⅠ位点,其它酶切位点出现的次数和位置都有细小差异;而不同PRV毒株gC基因均对G、C有明显的偏爱性。在蛋白质疏水性、抗原表位和二、三级结构的预测方面,不同毒株间具有相似性,但仍表现出一定程度的差异。
We got DNA of Pseudorabies virus Fa strain, vaccine strain 783, Bartha and SA215, Sichuan wild strain SN, SS, SQ, SL and SCZ preservatived by Animal Biotechnology center of Sichuan Agriculture University, and Beijing BJ strain, Guangdong DG strain, Hubei HS strain donated by prof. Lou Gaoming of Shao Guan Institute Ying Dong Bioengineering College. DNA of the 12 Pseudorabies virus strains served as template, 12 strips of gC gene fragments of PRV were amplified by PCR. Reclaimed the product of PCR, ligated and allaxised into competent cell of E.coli DH5α, then sent to biology company to sequencing after accredited by colony PC R and biocatalyst cutting.
The 12 strips gC gene sequences of different PRV strains, as well as 6 strips gC genes sequences which were downloaded from GenBank were analyzed by bioinformatics software. Analyzed and predicted that, such as nucleotide homology, location of mutation area, relationship of heredity and evolution, discrepancy of biocatalyst cutting site, preference of codon, homology of amino acid sequence, protein hydrophilicity, epitope, secondary structure and tertiary structure. The results showed that the length of open reading frame of gC gene was between 1437bp and1464bp, the number of amino acid encoded correspondingly was between 478 and 487. The ribonucleotide autoploidy was between 94.9% and 100%, the deduced autoploidy of amino acid sequence was between 90.2 % and 100%.There was a concentrated mutation area and a nucleotide deletion area on the 175-222 location segment of the nucleotide sequence, 8 basic groups mutated and 21 basic groups inset or absented. To analyze on the heredity and evolution relationship, the genetic relationship of the pseudorabies vims strains prevalent in Sichuan were closer to the pseudorabies vims strains isolated in Japan, Europe and America, but distant to the strains isolated in some region of our country. There was no HindIII biocatalyst cutting site in all 18 strips of gC gene, and there was no BamHI biocatalyst cutting site in Fa strain, DG strain ,SA215 strain ,BJ strain ,Min A strain and Ea strain either.Yamagata S-81 strain had no SalI site. There were tiny discrepancy on the occurrence frequency and situs of other biocatalyst cutting sites, and the gC gene of all strains had apparente preference to G and C. There was resemblance in proteo-hydrophobicity, epitope and higher structure prediction among those strains, but also had some changes and difference.
将所得12条序列连同GenBank中登录的6条gC基因全序列共18条基因序列,使用生物软件对gC基因核苷酸序列的同源性、突变区域的定位、遗传进化关系、酶切位点的差异、密码子偏爱性、氨基酸序列的同源性、蛋白质亲水性、抗原表位分析、二级结构和三级结构预测等生物信息学内容进行分析。结果表明:PRV-gC基因的开放阅读框的核苷酸长度在1437bp～1464bp之间,氨基酸长度在478～487个之间,核苷酸同源性在94.9%～100%之间,氨基酸的同源性在90.2%～100%之间;在核酸175～222位存在高变缺失区,其中共有8个碱基突变和21个碱基的插入或缺失;在遗传进化关系上,四川地区流行的毒株与日本、欧美毒株有较近的亲缘关系,而我国其它地区的毒株间的遗传相关性较高,但与日本、欧美洲毒株亲缘关系较远;在18条PRV-gC基因核苷酸序列中均没有发现HindⅢ酶切位点,同时Fa株、DG株、SA215株、BJ株、Min A株和Ea株也没有BamHⅠ位点,Yamagata S-81株则没有SalⅠ位点,其它酶切位点出现的次数和位置都有细小差异;而不同PRV毒株gC基因均对G、C有明显的偏爱性。在蛋白质疏水性、抗原表位和二、三级结构的预测方面,不同毒株间具有相似性,但仍表现出一定程度的差异。
We got DNA of Pseudorabies virus Fa strain, vaccine strain 783, Bartha and SA215, Sichuan wild strain SN, SS, SQ, SL and SCZ preservatived by Animal Biotechnology center of Sichuan Agriculture University, and Beijing BJ strain, Guangdong DG strain, Hubei HS strain donated by prof. Lou Gaoming of Shao Guan Institute Ying Dong Bioengineering College. DNA of the 12 Pseudorabies virus strains served as template, 12 strips of gC gene fragments of PRV were amplified by PCR. Reclaimed the product of PCR, ligated and allaxised into competent cell of E.coli DH5α, then sent to biology company to sequencing after accredited by colony PC R and biocatalyst cutting.
The 12 strips gC gene sequences of different PRV strains, as well as 6 strips gC genes sequences which were downloaded from GenBank were analyzed by bioinformatics software. Analyzed and predicted that, such as nucleotide homology, location of mutation area, relationship of heredity and evolution, discrepancy of biocatalyst cutting site, preference of codon, homology of amino acid sequence, protein hydrophilicity, epitope, secondary structure and tertiary structure. The results showed that the length of open reading frame of gC gene was between 1437bp and1464bp, the number of amino acid encoded correspondingly was between 478 and 487. The ribonucleotide autoploidy was between 94.9% and 100%, the deduced autoploidy of amino acid sequence was between 90.2 % and 100%.There was a concentrated mutation area and a nucleotide deletion area on the 175-222 location segment of the nucleotide sequence, 8 basic groups mutated and 21 basic groups inset or absented. To analyze on the heredity and evolution relationship, the genetic relationship of the pseudorabies vims strains prevalent in Sichuan were closer to the pseudorabies vims strains isolated in Japan, Europe and America, but distant to the strains isolated in some region of our country. There was no HindIII biocatalyst cutting site in all 18 strips of gC gene, and there was no BamHI biocatalyst cutting site in Fa strain, DG strain ,SA215 strain ,BJ strain ,Min A strain and Ea strain either.Yamagata S-81 strain had no SalI site. There were tiny discrepancy on the occurrence frequency and situs of other biocatalyst cutting sites, and the gC gene of all strains had apparente preference to G and C. There was resemblance in proteo-hydrophobicity, epitope and higher structure prediction among those strains, but also had some changes and difference.
引文
[1]殷震,刘景华.动物病毒学[M].北京:科技出版社,1997.329-340;998-1002
[2]Pensaert,M.B.and J.P.Kluge.1989.Pseudorabies virus(Aujeszky's disease)p39-64.In M.B Pensaert(ed.),Virus infections of porcines,Elsevier Science Publishing,BV,Amsterdam,The Netherlands.
[3]蔡宝祥.家畜传染病学[M].北京:中国农业出版社,1996.207-209
[4]郭万柱,颜其贵.伪狂犬病预防控制研究进展[A].畜禽重大疫病生物技术防制研究[C].北京:中国农业大学出版社,1998.156-166
[5]陈斌.伪狂犬病病毒Fa株胸苷激酶基因克隆及PCR扩增诊断伪狂犬病病毒的研究[D].雅安,四川农业大学,1996
[6]童光玉.猪伪狂犬病的诊断和防治[J].中国兽医杂志,1993,19(8):18-19
[7]李树春.仔猪伪狂犬病的诊断[J].中国兽医杂志,1995,8:21
[8]韦华姜.湖北首次暴发猪伪狂犬病的报告[J].中国畜禽传染病,1994,4:36-38
[9]朱庆虎.近10年我国伪狂犬病研究进展[J].中国畜禽传染病,1996,6:59-61
[10]Enquist,L.W.,P.J.Husak,B.W.Banfield and G.A.Smith.1999.Infection and spread of alphaherpesviruses in the nervous system.Adv.Virus Res.51:237-347
[11]Mettenleiter,T.C.2000.Aujeszky's disease(pseudorabies)virus:the virus and molecular pathogenesis-state of the art,June 1999.Vet.Res.31:99-115
[12]Mettenleiter,T.C.Herpesvirus assembly and egress.J.Virol.2002,76:1537-1547
[13]Mettenleiter,T.C.Initiation and spread of alpha-herpesvirus infections.Trends Microbiol.1994,2:2-4
[14]何启盖,陈焕春.猪伪狂犬病新的流行病学特征.华中农业大学学报.2000,增刊.
[15]Christensen L S.Evidence of long distance airborne transmission of Aujeszky's Disease (Pseudorabies) virus.Vet Rec,1990,127:471-474
[16]Gloster J,Donaldson A 1,Hough M N.Analysis of a series of outbreaks of Aujeszky's Disease in Yorkshire in 1981-82:The possibitity of airborne disease spread.Vet Rec,1984(114):234-239
[17]Schoenbaum M A,Zimmerman J J,Beran C Wet al.Survival of Psendorabies virus in aerosol.AJVR,1990(51):331-333
[18]程道祥.猪伪狂犬病的感染、传播和流行.福建畜牧兽医,1991(1):34
[19]刘正飞.伪狂犬病TK/gE基因缺失疫苗研究.武汉:华中农业大学,2002
[20]李成,刘树森,王继科等.畜禽病毒图谱[M].北京农业出版社,1987,1:5-16
[21]郭万柱,吴彤,陈瑶先.动物微生物学[M].成都:四川科学技术出版社,2001,327-328
[22]郭万柱,刘亚刚,娄高明.兽医病毒学[M].成都:四川科学技术出版社,2003,140-142
[23]李三星.关于伪狂犬病[J].河北农业大学学报,1989,12(1):145-150
[24]许雁峰,郭万柱,石谦等.猪伪狂犬病病毒gD基因的研究进展[J].四川畜牧兽医,2005,7:19-20
[25]陈世界,郭万柱.伪狂犬病病毒分子生物学[A].畜禽重大疫病生物技术防制研究[C].北京:中国农业大学出版社,1998.167.172
[26]Glenn ERall,Sabina Kupershmidt et al.Functions of the sequences at the ends of the inverted repeats of pseudorabies virus[J].Journal of Virology,Mar 1992,1506-1519
[27]Barbara G.Klupp,Christoph J.Hengarmer,Complete,Annotated Sequence of the Pseudorabi-es Virus Genome[J].Journal of Virology,2004(1):424-440
[28]余树民,郭万柱.伪狂犬病基因工程疫苗的研究现状[A].畜禽重大疫病生物技术防制研究[C].北京:中国农业大学出版社,1998,179一184
[29]王勤.伪狂犬病病毒Fa株gE基因的克隆与序列的比较分析[D].雅安,四川农业大学,2000
[30]Menttenleiter.T.C.Molecular biology of pseudorabies(Aujeszky's disease) virus.1991,14:11-163
[31]马相如.伪狂犬病病毒宿主关闭蛋白的基因克隆、表达及其结构分析[D].武汉,华中农业大学,2005
[32]Mayra E M Campbell et al.DNA sequences which regulate the expression of pseudorabies virus major immediate early gene[J].Virolgy(Vol157) 1994,307-326
[33]K L Glazenburg,B.RH.Peeters et al,Construction and properties of pseudorabies virus recombinants with altered control of immediate early gene expression[J].Journal of virology,jane 1995,189-197
[34]Suzette A,Priola Jack G Stevers.The 5'and 3'limits of transcription in the pseudorabies virus latency associated transcription unit[J].Virology,1991,182:852-856
[35]Andrew K Cheung et al.Pseudorabies virus deletion mutants involving the EP0 and LLT genes,US Patent,Oct 1994,No.5352596
[36]Gibson W.Structure and assembly of the virion[J].Inter virology,1996,39:389-400
[37]Fuchs W,Klupp B.G.,GranzowH,Hengartner C,Brack A,Mundt A,Enquist L.W,Metenleiter T C.Physicalin teraction between envelop glycoproteinE and M of pseudorabies virus and the major tegument protein UL49[J].J Virol,2002c,76(16):8208-8217
[38]Gershon A.A.,Sherman D.L.,Zhu Z,Gabel C.A.,Ambron R.T.,Gershon M.D.Intracellular transport of newly synthesized varieellazoster virus:f'mal envelopment in the trans-Golgi network[J].J Virol,2000,6 8:6372-6390
[39]Newcornb W N,Homa F L,Thomsen D R.Assembly of the herpes simplex virus procapsid from purified components and identification of small complexes containing the major capsid and scaffolding proteins[J].J Virol,1999,73:423-425
[40]Zhou Z,Chen D,Jakana J.Visualization of tegument-capsid interactions and DNA in intact herpes simplex virus type I virions[J].J Virol,1999,73:3210-3218
[41]娄高明,杜伟贤.郭万柱伪狂犬病病毒糖蛋白研究进展[J]广东畜牧兽医科技1999,24(4):3-6
[42]陈斌.伪狂犬病病毒及其免疫研究进展(上)[J].四川畜牧兽医2001,28(9):32-34
[43]Takad A,Kida H.Induction of protective antibody responwew against pseudorabies virus buintranasal vaccination with glycoproteinB inmice[J].Arch-virol,1995,140(9):1629-1635
[44]张志,范伟兴.赵宏坤伪狂犬病病毒囊膜糖蛋白的研究进展[J].山东农业大学学报(自然科学版),2001,32(1):85-89
[45]王勤.猪伪狂犬病病毒gE基因的研究进展[J].畜牧与兽医,2002,34(10):42-44
[46]范伟兴,赵宏坤.伪狂犬病病毒分子生物学研究新近展[A].中国畜牧兽医学会家畜传染病学分会第九次学术讨论会[C].2001
[47]Dijkstra.J.M.et al.Identification and characterization of pseudorabies virus glycoprotein gM as a nonessential virion component J.Virol.1996,70:5684-5688
[48]Jons.A et al.Gycoproteins M and N of pseudorabies virus form a disulfide-linked complex.J.Virol,1998,72:550-557
[49]王琴,郭万柱,余广海.伪狂犬病毒胸苷激酶基因缺失株的构建和应用的研究进展[J].中国畜禽传染病.1996,(3):61-64
[50]崔尚金,卫秀余,宋长绪等.当前猪伪狂犬病国内流行情况.动物科学与动物医学,2005(4):14-17
[51]金梅林,方六荣.伪狂犬病毒鄂A株的电镜形态观察.华中农业大学学报,2000,增刊
[52]李晓慧,孙柯楠,赵岭乐等.吉林省猪伪狂犬病诊断及病毒的分离与鉴定[J].吉林畜牧兽医,2006,27(9):9-10
[53]Stewart,W C.et al.Detection of pseudorabies vires by immunofluorescence.JAVMA,1967,151:747
[54]Allan G Met al.Rapid diagnosis of Aujeszky's disease in pig by immunofluorescence.Res. Vet.Sci.1984,36(2):235-239
[55]黄骏明,李亚香,朱庆虎等.应用免疫荧光抗体技术快速检测猪伪狂病病毒[J].中国畜禽传染病,1995,85(6):38-42
[56]Belaert F et al.Prevalence of herds with young sows seropositive to pseudorabies in northern Belgium[J].Prev Vet Med,1999,41(4):239-255
[57]方六荣,陈焕春,何启盖等.应用微量中和试验进行伪狂犬病血清学调查[J].中国畜禽传染病,1998,20(3):151-153
[58]李晓慧,胡业平,王为等.吉林省猪伪狂犬病血清学调查[J].中国畜牧兽医学会家畜传染病学分会第十次学术研讨会论文集,2003,431-432
[59]吴斌,陈焕春,方六荣等.乳胶凝集试验与血清中和试验检测猪伪狂犬病血清抗体的比较[J].中国兽医科技,1997,27(5):23 24
[60]程由铨,吴平,林天龙等.4种检测伪狂犬病病毒抗原方法的的比较[J].中国兽医杂志.1992,18(1):6-9
[61]娄高明,陈志荣,郭万柱等.双抗体夹心ELISA检测伪狂犬病病毒的研究[J].中国畜禽传染病,1998,20(4):236-240
[62]Morenkov O Set al.Indirect ELISAs based on recombinant and affinity purified glycoprotein E of Aujeszky's disease virus to differentiate between vaccinated and infected animals[J].Acta Vet Hung,1999,47(1):137-150
[63]郭万柱,冯炳芳,曹军等.应用~(32)p标记DNA探针检测伪狂犬病病病毒的研究[J].四川农业大学报.1991,9(I):52-56
[64]王琴,郭万柱.应用生物素标记DNA探针检测伪狂犬病病毒的研究[J].中国病毒学.1996,(11)3:284-285
[65]李学伍,陈焕春,周复春等.用地高辛标记的DNA探针检测伪狂犬病毒[J].畜牧与兽医.1997,29(6):270-271
[66]Belak S,L inne T.Rap id detection of Aujeszky's disease(pseudorabies) virus infection of p-igs by direct filter hybridisation of nasal and tonsillar specimens[J].Res Vet Sci,1988,44:303-308
[67]Maes R K,Sussman M D,Recent.Developments in Iatency and recombination of Aujeszky's disease (pseudorabies) virus[J].Vet Microbiol.1997,55:13-27
[68]Echeverria M G et al.Rapid diagnosis of pseudorabies virus infection in swine tissues using the polymerase chain reaction(PCR)[J].Rev Argent Microbiol,2000,32(3):109-155
[69]Harding M J et al.Specificity and nucleotyping studies of a gp50 based polymerase chain reaction assay for detection of pseudorabies virus[J].Can J Vet Res,1997,61:157-160
[70]石建平,宣华,卢强等.伪狂犬病PCR检测方法的建立及初步应用[J].中国畜禽传染病,1996,5:16-20
[71]吴斌,陈焕春,方六荣等.聚合酶链式反应在猪伪狂犬病临床诊断中的应用[J].中国兽医科技,1998,28(1):3-4
[72]李学伍,陈焕春,杨艳艳等.PCR法对伪狂犬病病猪不同部位的检测及敏感性试验[J]冲国畜禽传染病,1998,20(6):365-368
[73]陈钟鸣,张定东.猪伪狂犬病PCR诊断方法的建立[J].动物医学进展,2006,27(2):83-85
[74]Katz J Bet al.Molecular analysis of pseudorabies virus vaccines and their rapid differentiation from wild type isolates using DNA amplified glycoprotein E and thymidine kinase gene segment polymorphism[J].Biological,1992,20:187-195
[75]冉光智,童光志,孔令达等.利用复合多聚合酶链反应方法快速鉴别伪狂犬弱毒苗和野毒[J]冲国兽医杂志,1999,25(5):3-4
[76]刘丽娜,何启盖,陈焕春等.用多重PCR鉴别猪伪狂犬病野毒与疫苗毒的研究[J].中国兽医科技,2005,35(2):95-98
[77]Flynn S J.Ryan P A.heterologous heparin-binding domain can promote functional attachment pseudorabies virus gC mutant to cell surface[J].J-virol,1995,69(2):834-839
[78]Flynn S J.Ryan P.The receptor-binding domain of pseudotabies glycoprotein gC is composed of multiple discrete units that are functionlly redundant[J].J-viro 1,1996,70(3):1355-1364
[79]肖明.生物信息学及其在病毒研究中的应用[J].上海师范大学学报(自然科学版),2003,32(3),96-102
[80]Zhang G H,Ding D F.Search and Databank establishment[J].Acta of Biochemistry and Biophysics,1995,27(4):367-373
[81]王槐春.蛋白质与核酸序列分析的基础[M].北京:人民军队出版社,1994
[82]Henikoffs,Hehikoff J C.Amirto acid substitution matrices protein blocks[J].Proc Natl Acad Sci USA,1992,89:10915-10919
[83]Hehikoff J C,Pietrokovski S,Hehikoffs.Recent enhancements the Blocks Database servers [J].Nucleic Acids Research,1997,25(1):222-225
[84]Barroch A,Bucher P,Hafmamk.The PROSITE databases,its Etatus in1997[J]Nucleic Acids Research.1997,25(1):217-221
[85]Nevll-Manning C G,WU T D,Brutlag D L.Highly specific protein sequence motifs for genomes analysis[J].Proc.Natl.Acad.Sci.USA,1998,95:5865-5871
[86]Tatusov R L,Altschuil S F,Koonin E V.Detection of conserved segments in protein:Iterative scanning of sequence database with alignment blocks[J].Proc.Natl.Acad.Sci.USA,1994,91:12091-12095
[87]Ithimure D.DNA analysis.-New kids on the Block[J].Science,1999,285:355-356
[88]Strauss J H,Strauss E G.Viral RNA replication:with a little from the host[J].Science,1999,283:802-804
[89]Cardon L R,Storomo G D.Expectation maximization algorithm for identifying protern-binding sites with variable lengths from unaligned DNA fragments[J].J.Mol.Biol.1992,223:159-170
[90]Gribskow M,Mclachlan A D,Eiserberg D.Profileanalysis:detection of distantly related protein[J].Proc Natl Acad Sci USA,1987,84:4355-4358
[91]Schneider T D,Siormo G D,Gold L,et al.Information content of binding sites or nucleotide sequences[J].J.Mol.Biol,1986,188:415-431
[92]BercoD G,Von Hippel P.H,Selection of DNA binding sites by regulatory proteins:statistical:statistical-mechanicaltheoryandapplicationtooperationand promotes[J].J.Mol.Biol,1987,193:723-750
[93]Stormo G D,Hartzell G W.Identifying protein-binding sites from unaligned DNA fagments[J].Proc Natl Acad Sci USA,1989,86:1183-1187
[94]Stormo G D,Fields D S.Specificity,free energy and information content protein DNA interactionns[J].Trends in Biochemical Sciences,1998,23:109-113
[95]Fields D S,HE Y-YUAN,AL-UZRI A Y,et al.Quantitative specificity of the Mnt repressor[J].J Mol Bio1,1997,271:178-194
[96]Gelfand M S,Koonin E V,Mironov A A.Predication of transcription regulatory sites in Archace by a comparative genomic approach[J].Nucleic Acids Research,2000,28(3):695-705
[97]Mewes H W,Albermannk,Bahr M,et al.Overview of the yeast genome[J].Namre,1997,387(6632suppl):7-65
[98]Rota P A,Oberste M S,Monroe S S,et al.Characteraction of a novel cononavirus associated with severeacute respiratory syndrome[J].Science,2003,300:1394-1398
[99]Marra M A,Jones S J M,Astell C R.et al.The genome sequence of the sars-associated comnavirus [J].Science,2003,300:1399-1404
[100]Barbara G.Klupp,Christoph J.Hengartner,Complete,Annotated Sequence of the Pseudorabies Virus Genome[J].Journal of Virology,2004(1):424-440
[101]杨丽,郭万柱,殷华平等.伪狂犬病病毒密码子用法特点分[J].中国预防兽医学报,2007,29(2):103-106
[102]TrybaLa E,et al.Interaction between pseudorabies virus and heparin/heparan sulfate.J Biol.Chem,1998,237(9):5047-5052
[103]娄高明,敖敬群,廖筱萍等.伪狂犬病病毒闽A株糖蛋白gc基因全序列的克隆与序列分析[A].中国畜牧兽医学会畜牧兽医生物技术学分会第六次学术研讨会论文集[C].2001
[2]Pensaert,M.B.and J.P.Kluge.1989.Pseudorabies virus(Aujeszky's disease)p39-64.In M.B Pensaert(ed.),Virus infections of porcines,Elsevier Science Publishing,BV,Amsterdam,The Netherlands.
[3]蔡宝祥.家畜传染病学[M].北京:中国农业出版社,1996.207-209
[4]郭万柱,颜其贵.伪狂犬病预防控制研究进展[A].畜禽重大疫病生物技术防制研究[C].北京:中国农业大学出版社,1998.156-166
[5]陈斌.伪狂犬病病毒Fa株胸苷激酶基因克隆及PCR扩增诊断伪狂犬病病毒的研究[D].雅安,四川农业大学,1996
[6]童光玉.猪伪狂犬病的诊断和防治[J].中国兽医杂志,1993,19(8):18-19
[7]李树春.仔猪伪狂犬病的诊断[J].中国兽医杂志,1995,8:21
[8]韦华姜.湖北首次暴发猪伪狂犬病的报告[J].中国畜禽传染病,1994,4:36-38
[9]朱庆虎.近10年我国伪狂犬病研究进展[J].中国畜禽传染病,1996,6:59-61
[10]Enquist,L.W.,P.J.Husak,B.W.Banfield and G.A.Smith.1999.Infection and spread of alphaherpesviruses in the nervous system.Adv.Virus Res.51:237-347
[11]Mettenleiter,T.C.2000.Aujeszky's disease(pseudorabies)virus:the virus and molecular pathogenesis-state of the art,June 1999.Vet.Res.31:99-115
[12]Mettenleiter,T.C.Herpesvirus assembly and egress.J.Virol.2002,76:1537-1547
[13]Mettenleiter,T.C.Initiation and spread of alpha-herpesvirus infections.Trends Microbiol.1994,2:2-4
[14]何启盖,陈焕春.猪伪狂犬病新的流行病学特征.华中农业大学学报.2000,增刊.
[15]Christensen L S.Evidence of long distance airborne transmission of Aujeszky's Disease (Pseudorabies) virus.Vet Rec,1990,127:471-474
[16]Gloster J,Donaldson A 1,Hough M N.Analysis of a series of outbreaks of Aujeszky's Disease in Yorkshire in 1981-82:The possibitity of airborne disease spread.Vet Rec,1984(114):234-239
[17]Schoenbaum M A,Zimmerman J J,Beran C Wet al.Survival of Psendorabies virus in aerosol.AJVR,1990(51):331-333
[18]程道祥.猪伪狂犬病的感染、传播和流行.福建畜牧兽医,1991(1):34
[19]刘正飞.伪狂犬病TK/gE基因缺失疫苗研究.武汉:华中农业大学,2002
[20]李成,刘树森,王继科等.畜禽病毒图谱[M].北京农业出版社,1987,1:5-16
[21]郭万柱,吴彤,陈瑶先.动物微生物学[M].成都:四川科学技术出版社,2001,327-328
[22]郭万柱,刘亚刚,娄高明.兽医病毒学[M].成都:四川科学技术出版社,2003,140-142
[23]李三星.关于伪狂犬病[J].河北农业大学学报,1989,12(1):145-150
[24]许雁峰,郭万柱,石谦等.猪伪狂犬病病毒gD基因的研究进展[J].四川畜牧兽医,2005,7:19-20
[25]陈世界,郭万柱.伪狂犬病病毒分子生物学[A].畜禽重大疫病生物技术防制研究[C].北京:中国农业大学出版社,1998.167.172
[26]Glenn ERall,Sabina Kupershmidt et al.Functions of the sequences at the ends of the inverted repeats of pseudorabies virus[J].Journal of Virology,Mar 1992,1506-1519
[27]Barbara G.Klupp,Christoph J.Hengarmer,Complete,Annotated Sequence of the Pseudorabi-es Virus Genome[J].Journal of Virology,2004(1):424-440
[28]余树民,郭万柱.伪狂犬病基因工程疫苗的研究现状[A].畜禽重大疫病生物技术防制研究[C].北京:中国农业大学出版社,1998,179一184
[29]王勤.伪狂犬病病毒Fa株gE基因的克隆与序列的比较分析[D].雅安,四川农业大学,2000
[30]Menttenleiter.T.C.Molecular biology of pseudorabies(Aujeszky's disease) virus.1991,14:11-163
[31]马相如.伪狂犬病病毒宿主关闭蛋白的基因克隆、表达及其结构分析[D].武汉,华中农业大学,2005
[32]Mayra E M Campbell et al.DNA sequences which regulate the expression of pseudorabies virus major immediate early gene[J].Virolgy(Vol157) 1994,307-326
[33]K L Glazenburg,B.RH.Peeters et al,Construction and properties of pseudorabies virus recombinants with altered control of immediate early gene expression[J].Journal of virology,jane 1995,189-197
[34]Suzette A,Priola Jack G Stevers.The 5'and 3'limits of transcription in the pseudorabies virus latency associated transcription unit[J].Virology,1991,182:852-856
[35]Andrew K Cheung et al.Pseudorabies virus deletion mutants involving the EP0 and LLT genes,US Patent,Oct 1994,No.5352596
[36]Gibson W.Structure and assembly of the virion[J].Inter virology,1996,39:389-400
[37]Fuchs W,Klupp B.G.,GranzowH,Hengartner C,Brack A,Mundt A,Enquist L.W,Metenleiter T C.Physicalin teraction between envelop glycoproteinE and M of pseudorabies virus and the major tegument protein UL49[J].J Virol,2002c,76(16):8208-8217
[38]Gershon A.A.,Sherman D.L.,Zhu Z,Gabel C.A.,Ambron R.T.,Gershon M.D.Intracellular transport of newly synthesized varieellazoster virus:f'mal envelopment in the trans-Golgi network[J].J Virol,2000,6 8:6372-6390
[39]Newcornb W N,Homa F L,Thomsen D R.Assembly of the herpes simplex virus procapsid from purified components and identification of small complexes containing the major capsid and scaffolding proteins[J].J Virol,1999,73:423-425
[40]Zhou Z,Chen D,Jakana J.Visualization of tegument-capsid interactions and DNA in intact herpes simplex virus type I virions[J].J Virol,1999,73:3210-3218
[41]娄高明,杜伟贤.郭万柱伪狂犬病病毒糖蛋白研究进展[J]广东畜牧兽医科技1999,24(4):3-6
[42]陈斌.伪狂犬病病毒及其免疫研究进展(上)[J].四川畜牧兽医2001,28(9):32-34
[43]Takad A,Kida H.Induction of protective antibody responwew against pseudorabies virus buintranasal vaccination with glycoproteinB inmice[J].Arch-virol,1995,140(9):1629-1635
[44]张志,范伟兴.赵宏坤伪狂犬病病毒囊膜糖蛋白的研究进展[J].山东农业大学学报(自然科学版),2001,32(1):85-89
[45]王勤.猪伪狂犬病病毒gE基因的研究进展[J].畜牧与兽医,2002,34(10):42-44
[46]范伟兴,赵宏坤.伪狂犬病病毒分子生物学研究新近展[A].中国畜牧兽医学会家畜传染病学分会第九次学术讨论会[C].2001
[47]Dijkstra.J.M.et al.Identification and characterization of pseudorabies virus glycoprotein gM as a nonessential virion component J.Virol.1996,70:5684-5688
[48]Jons.A et al.Gycoproteins M and N of pseudorabies virus form a disulfide-linked complex.J.Virol,1998,72:550-557
[49]王琴,郭万柱,余广海.伪狂犬病毒胸苷激酶基因缺失株的构建和应用的研究进展[J].中国畜禽传染病.1996,(3):61-64
[50]崔尚金,卫秀余,宋长绪等.当前猪伪狂犬病国内流行情况.动物科学与动物医学,2005(4):14-17
[51]金梅林,方六荣.伪狂犬病毒鄂A株的电镜形态观察.华中农业大学学报,2000,增刊
[52]李晓慧,孙柯楠,赵岭乐等.吉林省猪伪狂犬病诊断及病毒的分离与鉴定[J].吉林畜牧兽医,2006,27(9):9-10
[53]Stewart,W C.et al.Detection of pseudorabies vires by immunofluorescence.JAVMA,1967,151:747
[54]Allan G Met al.Rapid diagnosis of Aujeszky's disease in pig by immunofluorescence.Res. Vet.Sci.1984,36(2):235-239
[55]黄骏明,李亚香,朱庆虎等.应用免疫荧光抗体技术快速检测猪伪狂病病毒[J].中国畜禽传染病,1995,85(6):38-42
[56]Belaert F et al.Prevalence of herds with young sows seropositive to pseudorabies in northern Belgium[J].Prev Vet Med,1999,41(4):239-255
[57]方六荣,陈焕春,何启盖等.应用微量中和试验进行伪狂犬病血清学调查[J].中国畜禽传染病,1998,20(3):151-153
[58]李晓慧,胡业平,王为等.吉林省猪伪狂犬病血清学调查[J].中国畜牧兽医学会家畜传染病学分会第十次学术研讨会论文集,2003,431-432
[59]吴斌,陈焕春,方六荣等.乳胶凝集试验与血清中和试验检测猪伪狂犬病血清抗体的比较[J].中国兽医科技,1997,27(5):23 24
[60]程由铨,吴平,林天龙等.4种检测伪狂犬病病毒抗原方法的的比较[J].中国兽医杂志.1992,18(1):6-9
[61]娄高明,陈志荣,郭万柱等.双抗体夹心ELISA检测伪狂犬病病毒的研究[J].中国畜禽传染病,1998,20(4):236-240
[62]Morenkov O Set al.Indirect ELISAs based on recombinant and affinity purified glycoprotein E of Aujeszky's disease virus to differentiate between vaccinated and infected animals[J].Acta Vet Hung,1999,47(1):137-150
[63]郭万柱,冯炳芳,曹军等.应用~(32)p标记DNA探针检测伪狂犬病病病毒的研究[J].四川农业大学报.1991,9(I):52-56
[64]王琴,郭万柱.应用生物素标记DNA探针检测伪狂犬病病毒的研究[J].中国病毒学.1996,(11)3:284-285
[65]李学伍,陈焕春,周复春等.用地高辛标记的DNA探针检测伪狂犬病毒[J].畜牧与兽医.1997,29(6):270-271
[66]Belak S,L inne T.Rap id detection of Aujeszky's disease(pseudorabies) virus infection of p-igs by direct filter hybridisation of nasal and tonsillar specimens[J].Res Vet Sci,1988,44:303-308
[67]Maes R K,Sussman M D,Recent.Developments in Iatency and recombination of Aujeszky's disease (pseudorabies) virus[J].Vet Microbiol.1997,55:13-27
[68]Echeverria M G et al.Rapid diagnosis of pseudorabies virus infection in swine tissues using the polymerase chain reaction(PCR)[J].Rev Argent Microbiol,2000,32(3):109-155
[69]Harding M J et al.Specificity and nucleotyping studies of a gp50 based polymerase chain reaction assay for detection of pseudorabies virus[J].Can J Vet Res,1997,61:157-160
[70]石建平,宣华,卢强等.伪狂犬病PCR检测方法的建立及初步应用[J].中国畜禽传染病,1996,5:16-20
[71]吴斌,陈焕春,方六荣等.聚合酶链式反应在猪伪狂犬病临床诊断中的应用[J].中国兽医科技,1998,28(1):3-4
[72]李学伍,陈焕春,杨艳艳等.PCR法对伪狂犬病病猪不同部位的检测及敏感性试验[J]冲国畜禽传染病,1998,20(6):365-368
[73]陈钟鸣,张定东.猪伪狂犬病PCR诊断方法的建立[J].动物医学进展,2006,27(2):83-85
[74]Katz J Bet al.Molecular analysis of pseudorabies virus vaccines and their rapid differentiation from wild type isolates using DNA amplified glycoprotein E and thymidine kinase gene segment polymorphism[J].Biological,1992,20:187-195
[75]冉光智,童光志,孔令达等.利用复合多聚合酶链反应方法快速鉴别伪狂犬弱毒苗和野毒[J]冲国兽医杂志,1999,25(5):3-4
[76]刘丽娜,何启盖,陈焕春等.用多重PCR鉴别猪伪狂犬病野毒与疫苗毒的研究[J].中国兽医科技,2005,35(2):95-98
[77]Flynn S J.Ryan P A.heterologous heparin-binding domain can promote functional attachment pseudorabies virus gC mutant to cell surface[J].J-virol,1995,69(2):834-839
[78]Flynn S J.Ryan P.The receptor-binding domain of pseudotabies glycoprotein gC is composed of multiple discrete units that are functionlly redundant[J].J-viro 1,1996,70(3):1355-1364
[79]肖明.生物信息学及其在病毒研究中的应用[J].上海师范大学学报(自然科学版),2003,32(3),96-102
[80]Zhang G H,Ding D F.Search and Databank establishment[J].Acta of Biochemistry and Biophysics,1995,27(4):367-373
[81]王槐春.蛋白质与核酸序列分析的基础[M].北京:人民军队出版社,1994
[82]Henikoffs,Hehikoff J C.Amirto acid substitution matrices protein blocks[J].Proc Natl Acad Sci USA,1992,89:10915-10919
[83]Hehikoff J C,Pietrokovski S,Hehikoffs.Recent enhancements the Blocks Database servers [J].Nucleic Acids Research,1997,25(1):222-225
[84]Barroch A,Bucher P,Hafmamk.The PROSITE databases,its Etatus in1997[J]Nucleic Acids Research.1997,25(1):217-221
[85]Nevll-Manning C G,WU T D,Brutlag D L.Highly specific protein sequence motifs for genomes analysis[J].Proc.Natl.Acad.Sci.USA,1998,95:5865-5871
[86]Tatusov R L,Altschuil S F,Koonin E V.Detection of conserved segments in protein:Iterative scanning of sequence database with alignment blocks[J].Proc.Natl.Acad.Sci.USA,1994,91:12091-12095
[87]Ithimure D.DNA analysis.-New kids on the Block[J].Science,1999,285:355-356
[88]Strauss J H,Strauss E G.Viral RNA replication:with a little from the host[J].Science,1999,283:802-804
[89]Cardon L R,Storomo G D.Expectation maximization algorithm for identifying protern-binding sites with variable lengths from unaligned DNA fragments[J].J.Mol.Biol.1992,223:159-170
[90]Gribskow M,Mclachlan A D,Eiserberg D.Profileanalysis:detection of distantly related protein[J].Proc Natl Acad Sci USA,1987,84:4355-4358
[91]Schneider T D,Siormo G D,Gold L,et al.Information content of binding sites or nucleotide sequences[J].J.Mol.Biol,1986,188:415-431
[92]BercoD G,Von Hippel P.H,Selection of DNA binding sites by regulatory proteins:statistical:statistical-mechanicaltheoryandapplicationtooperationand promotes[J].J.Mol.Biol,1987,193:723-750
[93]Stormo G D,Hartzell G W.Identifying protein-binding sites from unaligned DNA fagments[J].Proc Natl Acad Sci USA,1989,86:1183-1187
[94]Stormo G D,Fields D S.Specificity,free energy and information content protein DNA interactionns[J].Trends in Biochemical Sciences,1998,23:109-113
[95]Fields D S,HE Y-YUAN,AL-UZRI A Y,et al.Quantitative specificity of the Mnt repressor[J].J Mol Bio1,1997,271:178-194
[96]Gelfand M S,Koonin E V,Mironov A A.Predication of transcription regulatory sites in Archace by a comparative genomic approach[J].Nucleic Acids Research,2000,28(3):695-705
[97]Mewes H W,Albermannk,Bahr M,et al.Overview of the yeast genome[J].Namre,1997,387(6632suppl):7-65
[98]Rota P A,Oberste M S,Monroe S S,et al.Characteraction of a novel cononavirus associated with severeacute respiratory syndrome[J].Science,2003,300:1394-1398
[99]Marra M A,Jones S J M,Astell C R.et al.The genome sequence of the sars-associated comnavirus [J].Science,2003,300:1399-1404
[100]Barbara G.Klupp,Christoph J.Hengartner,Complete,Annotated Sequence of the Pseudorabies Virus Genome[J].Journal of Virology,2004(1):424-440
[101]杨丽,郭万柱,殷华平等.伪狂犬病病毒密码子用法特点分[J].中国预防兽医学报,2007,29(2):103-106
[102]TrybaLa E,et al.Interaction between pseudorabies virus and heparin/heparan sulfate.J Biol.Chem,1998,237(9):5047-5052
[103]娄高明,敖敬群,廖筱萍等.伪狂犬病病毒闽A株糖蛋白gc基因全序列的克隆与序列分析[A].中国畜牧兽医学会畜牧兽医生物技术学分会第六次学术研讨会论文集[C].2001
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