摘要
环境激素又称为内分泌干扰物,是一种由于人类的生产和生活活动而释放到周围环境中的,对人体内和动物体内原本营造的正常激素功能施加影响,从而影响内分泌系统的化学物质,使人类和生物的持续生存和繁衍受到威胁。因此建立一套操作简便省时省力的痕量检测方法及研究出它们与DNA的反应模式,不仅有一定的现实意义也为从分子水平上认识环境激素的致毒机理和评估危害提供依据。
本文用溶剂浮选法首先对甲氰菊酯、溴氰菊酯、联苯菊酯、氯氰菊酯、氰戊菊酯、氯菊酯、氟胺氰菊酯、氟氯氰菊酯及双酚A、烷基酚、辛基酚进行分离富集,然后用高效液相色谱法进行定性定量分析。分别考察了浮选溶剂、试液pH、氮气流速及浮选时间等因素对溶剂浮选效率的影响,应用本文所建立的溶剂浮选-高效液相色谱法,实现了对燕山石化地区水体中菊酯类、烷基酚和双酚A环境激素的测定,实验结果令人满意。同时,用荧光光谱法和紫外光谱法,研究了辛基酚、壬基酚、双酚A、甲氰菊酯、溴氰菊酯、联苯菊酯与DNA相互作用的机理,考察了猝灭剂、磷酸根的影响,结果表明:辛基酚、壬基酚、双酚A、甲氰菊酯、溴氰菊酯、联苯菊酯与DNA均形成加合物,从而会对生物体的DNA产生化学损伤最终导致基因突变。
Environmental hormones, also was called environmental endocrine disruption chemicals, acted as estrogens in human and animal bodies. Most of them were discharged into the environment with the production and the life of the humanity, which would pose a threat to the sustaind living and proliferation of humans and animals. So accomplishing fast monitoring of the environmental contamination analytical methods and studying the interaction mechanism of ctDNA with them are not only practical significance but also providing basis with poison mechanism of environmental hormones.
In this paper, fenpropathrin (FP), deltamethrin (DL), bifenthrin (BF), cypermethrin (CM), fenvalerate (FV), permethrin (PM), fluvalinate (FL), cyhalothrin (CH) and bisphenol A, octyphenol and nonylphenol in water by solvent sublation and their determination by high performance liquid chromatography has been developed. The effects of sublation solvent, pH of the solution, nitrogen flow rate and sublation time were discussed; on the sublation efficiency were investigated in detail, and the optimal conditions of the solvent sublation were obtained. The proposed method was used to determine the contents of pyrethroid insecticides, bisphenol A, octyphenol and nonylphenol in the water sample from Beijing Yanshan Petrochemical District. The results show that this method is satisfactory. While, the interaction mechanism of ctDNA with fenpropathrin (FP), deltamethrin (DL), bifenthrin (BF), bisphenol A (BPA), octyphenol (OP) and nonylphenol (NP) was studied by fluorescence and UV spectrometry. The influence of phosphate, quencher and interaction force was discussed. The results demonstrate that they can interact with ctDNA, which implied that they could bind to ctDNA to produce DNA adducts. This might result in the gene mutation and bring the chemical trauma to the DNA of the biologic bodies.
引文
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