小麦条锈菌PsMAPK1，PsCdc2，PsFuz7基因的克隆及PsMAPK1基因功能验证
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摘要
本论文研究了在小麦与条锈菌互作过程中,条锈菌催分裂原活化蛋白激酶基因PsMAPK1、细胞分裂周期基因PsCdc2及催分裂原活化蛋白激酶的激酶基因PsFuz7的转录表达情况;分析了PsMAPK1和PsFuz7基因在小麦条锈菌不同的生理小种中氨基酸序列的差异;另外,借助小麦赤霉病菌和稻瘟病菌突变体对PsMAPK1基因进行基因功能的验证。
本论文首次从已构建的文库中筛选出小麦条锈菌催分裂原活化蛋白激酶基因PsMAPK1、细胞周期分裂基因PsCdc2和催分裂原活化蛋白激酶的激酶基因PsFuz7。分别克隆得到三个基因的cDNA和基因组DNA的全长,其中PsMAPK1基因组DNA全长2343bp,cDNA序列全长1834 bp,ORF编码408个氨基酸,由8个外显子和7个内含子组成;PsCdc2基因组DNA长2279bp ,cDNA序列全长1395 bp,编码294个氨基酸,由11个外显子和10个内含子组成;PsFuz7基因组DNA全长2661bp,cDNA序列全长2093 bp,编码419个氨基酸,含由8个外显子和7个内含子组成。
定量表达分析表明,PsMAPK1在条锈菌侵染小麦前期呈上调表达,其中24h时表达量最高约为夏孢子中表达量的5.32倍,侵染36 h后表达量明显下降。PsCdc2在条锈菌夏孢子及病菌侵染后各个时间点均有表达,其中侵染12 h之前表达量称上升趋势,侵染12 h时表达量最高,约为夏孢子中表达量的1.62倍,侵染18 h之后表达量呈下降趋势,侵染36 h之后表达量均低于夏孢子中的表达量。PsFuz7在条锈菌侵染小麦后12 h前呈上调表达趋势,侵染后12 h时的表达量最高,约为夏孢子中表达量的1.95倍,侵染后6 h时表达量约为夏孢子中表达量的1.79倍,侵染18 h后表达量下降,表达量均低于夏孢子中的表达量。小麦条锈菌不同生理小种间序列分析发现,PsMAPK1和PsFuz7在条锈菌生理小种CYR23、CYR25、CYR29、CYR31、CYR32、CYR33中均非常保守,核苷酸序列间无大的差异,PsMAPK1有9个氨基酸位点可能为易变位点,PsMAPK1有12个位点可能为易变位点。
功能互补分析表明,PsMAPK1基因能够部分恢复小麦赤霉病菌△map1突变体的生长特性和致病性,能够部分恢复稻瘟菌突变体附着孢的形成和致病性,结合定量分析推测PsMAPK1基因具有与其他真菌MAPK基因功能上的一致性和保守性,为条锈病菌上一个致病相关基因。同时,通过对生物信息学分析及定量分析,推测PsFuz7、PsCdc2均为小麦条锈菌上重要的致病相关基因。PsFuz7可能通过催分裂原活化蛋白激酶级联反应,在将细胞外信号传递到细胞内,最终导致转录因子的激活从而调控致病性;而PsCdc2可能通过调节条锈菌的细胞周期,参与条锈菌侵染小麦前期菌丝的生长。
In this paper, some explanations have been given about the transcriptional profilings of mitogen-activated protein kinase (PsMAPK1), cell division cycle 2 (PsCdc2) and mitogen-activated protein kinase kinase PsFUZ7. The differentiation of PsMAPK1 and PsFUZ7 amino acid sequences in stripe rust CYR23, CYR25, CYR29, CYR31 CYR32 and CYR33 were also reported. In addition, we described the gene potential function of PsMAPK1 by complementation of the Fusarium graminearum mutant and Magnaporthe grisea mutant.
Mitogen-activated protein kinase MAPK1, cell division cycle Cdc2 and mitogen-activated protein kinase kinase FUZ7 from Puccinia striiformis f.sp. tritici (Pst) were firstly isolated through screeming the DNA library. We tentatively designated these three gene as PsMAPK1, PsCdc2 and PsFUZ7. The cDNA sequence of PsMAPK1 is 1834 bp contain a 1227 bp open reading frame and encoded a putative protein composed of 408 amino acids. The DNA sequence of PsMAPK1 is 2343 bp, comprised of 8 extrons and 7 introns. The cDNA sequence of PsCdc2 is 1395 bp contain a 885 bp open reading frame and encoded a putative protein composed of 294 amino acids. The DNA sequence of PsMAPK1 is 2279 bp, comprised of 8 extrons and 7 introns.
The cDNA sequence of PsFuz7 is 2093 bp contain a 1260 bp open reading frame and encoded a putative protein composed of 419 amino acids. The DNA sequence of PsMAPK1 is 2661 bp, comprised of 8 extrons and 7 introns. Real time RT-PCR analysis showed that PsMAPK1 was up-regulated at early stage of infection, the maximum induction occurred at 24 hpi, at which transcripts were 5.32 fold over that in urediniospore. The accumulation of transcripts decreased obviously after 36 hpi. The maximum induction of PsCdc2 occurred at 12 hpi, the transcripts were 1.62 fold over that in urediniospore. The accumulation of transcripts decreased steadily after 12 hpi. PsFuz7 was up-regulated at early infection(before 12 hpi), The maximum induction were 1.95 fold over that in urediniospore., which occurred at 12 hpi. The induction at 6 hpi was 1.79 fold over that in urediniospore, and after 18 hpi, the induction were all lower than that in urediniospore. Sequences analysis of PsMAPK in different stripe rust race, CYR23, CYR25, CYR29, CYR31, CYR32 and CYR33, showed that there was no obvious differentiations, but there might be 9 changeable mutant locations. PsFuz7 also had no obvious differentiations, but there might be 12 changeable mutant locations. In addition, PsMAPK1 can complement the the Fusarium graminearum mutant and Magnaporthe grisea mutant. PsMAPK1 might be essennntial for pathogenicity and infectious growth in stripe rust fungi.
本论文首次从已构建的文库中筛选出小麦条锈菌催分裂原活化蛋白激酶基因PsMAPK1、细胞周期分裂基因PsCdc2和催分裂原活化蛋白激酶的激酶基因PsFuz7。分别克隆得到三个基因的cDNA和基因组DNA的全长,其中PsMAPK1基因组DNA全长2343bp,cDNA序列全长1834 bp,ORF编码408个氨基酸,由8个外显子和7个内含子组成;PsCdc2基因组DNA长2279bp ,cDNA序列全长1395 bp,编码294个氨基酸,由11个外显子和10个内含子组成;PsFuz7基因组DNA全长2661bp,cDNA序列全长2093 bp,编码419个氨基酸,含由8个外显子和7个内含子组成。
定量表达分析表明,PsMAPK1在条锈菌侵染小麦前期呈上调表达,其中24h时表达量最高约为夏孢子中表达量的5.32倍,侵染36 h后表达量明显下降。PsCdc2在条锈菌夏孢子及病菌侵染后各个时间点均有表达,其中侵染12 h之前表达量称上升趋势,侵染12 h时表达量最高,约为夏孢子中表达量的1.62倍,侵染18 h之后表达量呈下降趋势,侵染36 h之后表达量均低于夏孢子中的表达量。PsFuz7在条锈菌侵染小麦后12 h前呈上调表达趋势,侵染后12 h时的表达量最高,约为夏孢子中表达量的1.95倍,侵染后6 h时表达量约为夏孢子中表达量的1.79倍,侵染18 h后表达量下降,表达量均低于夏孢子中的表达量。小麦条锈菌不同生理小种间序列分析发现,PsMAPK1和PsFuz7在条锈菌生理小种CYR23、CYR25、CYR29、CYR31、CYR32、CYR33中均非常保守,核苷酸序列间无大的差异,PsMAPK1有9个氨基酸位点可能为易变位点,PsMAPK1有12个位点可能为易变位点。
功能互补分析表明,PsMAPK1基因能够部分恢复小麦赤霉病菌△map1突变体的生长特性和致病性,能够部分恢复稻瘟菌突变体附着孢的形成和致病性,结合定量分析推测PsMAPK1基因具有与其他真菌MAPK基因功能上的一致性和保守性,为条锈病菌上一个致病相关基因。同时,通过对生物信息学分析及定量分析,推测PsFuz7、PsCdc2均为小麦条锈菌上重要的致病相关基因。PsFuz7可能通过催分裂原活化蛋白激酶级联反应,在将细胞外信号传递到细胞内,最终导致转录因子的激活从而调控致病性;而PsCdc2可能通过调节条锈菌的细胞周期,参与条锈菌侵染小麦前期菌丝的生长。
In this paper, some explanations have been given about the transcriptional profilings of mitogen-activated protein kinase (PsMAPK1), cell division cycle 2 (PsCdc2) and mitogen-activated protein kinase kinase PsFUZ7. The differentiation of PsMAPK1 and PsFUZ7 amino acid sequences in stripe rust CYR23, CYR25, CYR29, CYR31 CYR32 and CYR33 were also reported. In addition, we described the gene potential function of PsMAPK1 by complementation of the Fusarium graminearum mutant and Magnaporthe grisea mutant.
Mitogen-activated protein kinase MAPK1, cell division cycle Cdc2 and mitogen-activated protein kinase kinase FUZ7 from Puccinia striiformis f.sp. tritici (Pst) were firstly isolated through screeming the DNA library. We tentatively designated these three gene as PsMAPK1, PsCdc2 and PsFUZ7. The cDNA sequence of PsMAPK1 is 1834 bp contain a 1227 bp open reading frame and encoded a putative protein composed of 408 amino acids. The DNA sequence of PsMAPK1 is 2343 bp, comprised of 8 extrons and 7 introns. The cDNA sequence of PsCdc2 is 1395 bp contain a 885 bp open reading frame and encoded a putative protein composed of 294 amino acids. The DNA sequence of PsMAPK1 is 2279 bp, comprised of 8 extrons and 7 introns.
The cDNA sequence of PsFuz7 is 2093 bp contain a 1260 bp open reading frame and encoded a putative protein composed of 419 amino acids. The DNA sequence of PsMAPK1 is 2661 bp, comprised of 8 extrons and 7 introns. Real time RT-PCR analysis showed that PsMAPK1 was up-regulated at early stage of infection, the maximum induction occurred at 24 hpi, at which transcripts were 5.32 fold over that in urediniospore. The accumulation of transcripts decreased obviously after 36 hpi. The maximum induction of PsCdc2 occurred at 12 hpi, the transcripts were 1.62 fold over that in urediniospore. The accumulation of transcripts decreased steadily after 12 hpi. PsFuz7 was up-regulated at early infection(before 12 hpi), The maximum induction were 1.95 fold over that in urediniospore., which occurred at 12 hpi. The induction at 6 hpi was 1.79 fold over that in urediniospore, and after 18 hpi, the induction were all lower than that in urediniospore. Sequences analysis of PsMAPK in different stripe rust race, CYR23, CYR25, CYR29, CYR31, CYR32 and CYR33, showed that there was no obvious differentiations, but there might be 9 changeable mutant locations. PsFuz7 also had no obvious differentiations, but there might be 12 changeable mutant locations. In addition, PsMAPK1 can complement the the Fusarium graminearum mutant and Magnaporthe grisea mutant. PsMAPK1 might be essennntial for pathogenicity and infectious growth in stripe rust fungi.
引文
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