瓜果腐霉代谢产物中除草活性物质的分离与鉴定
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摘要
瓜果腐霉(Pythium aphanidermatum(Eds)Fitzp)属于卵菌门,寄主范围较广,可引起多种植物的猝倒病和瓜果腐烂病。木实验室从黑麦草上分离到一株具有一定除草活性的瓜果腐霉菌株PA1,本试验以PA1以及分离自黄瓜的PAC菌株为出发菌株,通过发酵对菌株的除草活性物质的产生、分离及结构鉴定进行了系统研究。
经对瓜果腐霉进行诱导、分离培养,得到了PAC、PAE等4个菌株,利用种子萌发法、生长抑制作用测定法对实验室保存的PA1及上述得到的4个菌株进行了除草活性测定,结果表明,来自黑麦草的PA1和来自黄瓜的PAC菌株分别对马唐和反枝苋表现出了较强的除草活性,其他3个菌株则活性不明显。
对菌株PA1和PAC进行紫外诱变,分别得到了31株和33株诱变菌株,并对其除草活性进行了测定,结果表明,PA1菌株诱变后只有少数菌株的除草活性增强,如PA1-M1和PA1-M4菌株,多数菌株活性变化不明显;PAC菌株经紫外诱变不同时间处理后得到的菌株数量不等,除草活性结果表明,除草活性增强的菌株较多,其中PAC-M2对反枝苋的生长抑制作用较强。
利用麦麸、小米、玉米等6种固体培养基质对瓜果腐霉的PA1-M1和PAC-M2菌株进行了固体发酵,经生物测定并结合TLC和HPLC,最终选定麦麸作为大量固体发酵的培养基质,在此基础上,又向麦麸培养基中加入适量的牛肉膏、蛋白胨和酵母膏,发现麦麸中加入蛋白胨可作为瓜果腐霉产生除草活性物质的最适培养基。
利用PD培养基对PA1-M1菌株进行了液体发酵,比较了震荡和静置两种培养方式,以及培养滤液初始pH对菌丝干重和培养滤液除草活性的影响,结果表明,振荡方式、初始pH为5～7时,菌丝的产生量最大,培养滤液的除草活性也最强。
对菌株PA1-M1和PAC-M2的培养滤液粗提物进行了除草活性测定,结果表明,PA1-M1培养滤液粗提物对马唐根芽的IC_(50)分别为0.36mg/mL和0.46mg/mL,PAC-M2培养滤液粗提物对反枝苋根和芽的IC_(50)分别为0.49mg/mL和0.36mg/mL。对PA1-M1菌株液体发酵后的培养滤液及粗提物进行了热稳定性、杀草谱的检测,发现该毒素对热稳定,对供试的马唐、反枝苋、藜等杂草均有较强的除草活性。两菌株菌丝的除草活性结果表明,PA1-M1菌丝对马唐根和芽的IC_(50)分别为7.36 mg/mL和4.09 mg/mL,PAC-M2菌丝粗提物对反枝苋根和芽和IC_(50)分别为3.74 mg/mL和4.76 mg/mL。
利用溶剂提取、树脂柱层析、TLC、硅胶柱层析、HPLC等分离技术,对瓜果腐霉PA1-M1菌株的液体发酵代谢产物进行了系统的研究。从菌丝中分离出具有除草活性的化合物1和化合物2:确定了培养滤液粗提物的洗脱方案为50%乙醇、95%乙醇、乙酸乙酯、丙酮依次洗脱;对所得的乙酸乙酯洗脱液,95%乙醇洗脱液和50%乙醇洗脱液分别采用不同的方法进行分离,得到了化合物3、化合物4和化合物5、化合物6,根据标准样品及其保留时间判断化合物4为邻苯二甲酸二甲酯。
对PA1-M1菌株的固体发酵产物利用乙酸乙酯提取,再经TLC、硅胶柱层析和HPLC分离,得到了化合物7、化合物8、化合物9、化合物10和化合物11。
利用红外光谱(IR)、核磁共振波谱(NMR)、液质联用(LC-MS)技术对所得到的化合物1、化合物2,化合物5～化合物10进行了结构分析,确定了化合物7、化合物8、化合物9、化合物10的结构分别为阿魏酸、2-(1-H-吲哚-3-基)丁酸乙酯、阿魏酸正丁酯和2-(1-H-吲哚-3-基)己酸乙酯。化合物1为含有两个羟基的不饱和长链脂肪酸,其分子量为578,分子式为C_(37)H_(70)O_4。化合物2与化合物1属于同一类。化合物5的分子量为306。
Pythium aphanidermatum is a kind of pathogen belongs to Oomycete,which can cause many diseases such as bed rot of many kinds of crops,melons and fruits. The previous results showed that PA1 of P.aphanidermatum,which was isolated from the infected Perennial ryegrass had herbicidal activity.In this study,PA1 and the other 4 isolates that isolated from different hosts were researched detailedly,such as the production,separation and structure identification of the herbicidal substances.
Different melons and fruits were used to isolate the pathogen P. aphanidermatum,and 4 isolates were obtained.Bioassy of the crude toxins produced by the 4 isolates and PA1 was tested by the methods of seed germination and inhibition on weed growth.The results showed that PA1 had the stronger herbicidal activity to Digitaria sanguinalis,and the isolate PAC had stronger activity to Amaranthus retroflexus.
31 and 33 mutant-isolates were obtained by irradiating the mycelia of PA1 and PAC under UV illumination and their herbicidal activity were researched.The results showed that only fewness mutant isolates as PA1-M1and PA1-M4 from PA1 had a increased herbicidal activity,and the activity of other isolates was nealy the same as PA1 or decreased.Different numbers of isolates were obtained from PAC when irradiated by different time,and the herbicidal activity of most of the mutant isolates was increased,and that of the isolate PAC-M2 was stronger to A.retroflexus.
The wheat bran,millet,rice,corn,oat and soybean meal were used as solid media to ferment PA1-M1 and PAC-M2,and the crude toxins were bioassyed and separated using the method of TLC and HPLC.The results showed that the herbicidal activity of the crude toxins from wheat bran was better than the others. HPLC analysis was done to the crude toxins from wheat bran after adding different nutration such as beef extracts,peptone,yeast extracts,and the results showed that the toxin production of that of peptone was highest,so the wheat bran,in which added some peptone was selected as the optimal solid culture for the PA1-M1 and PAC-M2.
PD media was used to ferment PA1 and PAC-M2,and the affection of culturing modes such as shaking and stillness,and pH of PD on the dry weight of mycelia and herbicidal activity of the filtrate were studied.The results showed that the more suitable condition for the producing of mycelia and filtrate were shaking culturing at intial pH5-7.
Herbicidal activity of filrate extracts and mycelia extracts produced by PA1-M1 and PAC-M2 was tested.The results showed that the IC_(50) of filtrate extracts from PA1-M1 to the root and bud of Digitaria sanguinalis was 0.36mg/mL and 0.46mg/mL respectively,while that from PAC-M2 were 0.49mg/mL and 0.36mg/mL respectively.The filtrate extracts were steady to heat and had herbicidal activity to the weeds tested.The IC(50) of mycelia extracts from PA1-M1 to the root and bud of Amaranthus retroflexus was 7.36 mg/mL and 4.09 mg/mL,and that from PAC-M2 was 3.74 mg/mL and 4.76 mg/mL respectively.
The metabolites produced by PA1 in liquid media was researched in detail using the method of solvent extacting,adsorbing by colophony,TLC,Silica Gel Column, Chromatography and HPLC.Compound 1 and compound 2 was obtained from the mycelia extracts.The eluting mode was determined as 50%ethanol,95%ethanol, ethyl acetate and acetone in turn,and the eluting fractions were separated with different methods.In this way,compound 3,compound 4,compound 5 and compound 6 were obtained respectively.The results of HPLC showed that compound 4 was determined as dimethyl 4-nitrophthalate.
Compound 7,compound 8,compound 9 and compound 10 were obtained by using ethyl acetate extrating,TLC,Silica Gel Column Chromatography and HPLC from the solid fermented extration.
IR,NMR,LC-MS were used to identify and analysis the chemical structure of the compounds obtained.The structure of compound 7,8,9 and 10 was ferulic acid, 2-(1H-indol-3-yl)ethyl butyrate,butyl-3-(3-methoxy-4-methylphenyl)acrylate,and 2-(1H-indol-3-yl)ethyl hexanoate,respectively.
Compound 1 was an unsaturated fatty acid with 2 hydroxyls and the molecular weight and molecular formula was 578 and C_(37)H_(70)O_4 respectively.Compound 2 was concluded to be the same type with componud 1,and MS spectrum indicated the molecular weight of compound 5 was 306.
经对瓜果腐霉进行诱导、分离培养,得到了PAC、PAE等4个菌株,利用种子萌发法、生长抑制作用测定法对实验室保存的PA1及上述得到的4个菌株进行了除草活性测定,结果表明,来自黑麦草的PA1和来自黄瓜的PAC菌株分别对马唐和反枝苋表现出了较强的除草活性,其他3个菌株则活性不明显。
对菌株PA1和PAC进行紫外诱变,分别得到了31株和33株诱变菌株,并对其除草活性进行了测定,结果表明,PA1菌株诱变后只有少数菌株的除草活性增强,如PA1-M1和PA1-M4菌株,多数菌株活性变化不明显;PAC菌株经紫外诱变不同时间处理后得到的菌株数量不等,除草活性结果表明,除草活性增强的菌株较多,其中PAC-M2对反枝苋的生长抑制作用较强。
利用麦麸、小米、玉米等6种固体培养基质对瓜果腐霉的PA1-M1和PAC-M2菌株进行了固体发酵,经生物测定并结合TLC和HPLC,最终选定麦麸作为大量固体发酵的培养基质,在此基础上,又向麦麸培养基中加入适量的牛肉膏、蛋白胨和酵母膏,发现麦麸中加入蛋白胨可作为瓜果腐霉产生除草活性物质的最适培养基。
利用PD培养基对PA1-M1菌株进行了液体发酵,比较了震荡和静置两种培养方式,以及培养滤液初始pH对菌丝干重和培养滤液除草活性的影响,结果表明,振荡方式、初始pH为5～7时,菌丝的产生量最大,培养滤液的除草活性也最强。
对菌株PA1-M1和PAC-M2的培养滤液粗提物进行了除草活性测定,结果表明,PA1-M1培养滤液粗提物对马唐根芽的IC_(50)分别为0.36mg/mL和0.46mg/mL,PAC-M2培养滤液粗提物对反枝苋根和芽的IC_(50)分别为0.49mg/mL和0.36mg/mL。对PA1-M1菌株液体发酵后的培养滤液及粗提物进行了热稳定性、杀草谱的检测,发现该毒素对热稳定,对供试的马唐、反枝苋、藜等杂草均有较强的除草活性。两菌株菌丝的除草活性结果表明,PA1-M1菌丝对马唐根和芽的IC_(50)分别为7.36 mg/mL和4.09 mg/mL,PAC-M2菌丝粗提物对反枝苋根和芽和IC_(50)分别为3.74 mg/mL和4.76 mg/mL。
利用溶剂提取、树脂柱层析、TLC、硅胶柱层析、HPLC等分离技术,对瓜果腐霉PA1-M1菌株的液体发酵代谢产物进行了系统的研究。从菌丝中分离出具有除草活性的化合物1和化合物2:确定了培养滤液粗提物的洗脱方案为50%乙醇、95%乙醇、乙酸乙酯、丙酮依次洗脱;对所得的乙酸乙酯洗脱液,95%乙醇洗脱液和50%乙醇洗脱液分别采用不同的方法进行分离,得到了化合物3、化合物4和化合物5、化合物6,根据标准样品及其保留时间判断化合物4为邻苯二甲酸二甲酯。
对PA1-M1菌株的固体发酵产物利用乙酸乙酯提取,再经TLC、硅胶柱层析和HPLC分离,得到了化合物7、化合物8、化合物9、化合物10和化合物11。
利用红外光谱(IR)、核磁共振波谱(NMR)、液质联用(LC-MS)技术对所得到的化合物1、化合物2,化合物5～化合物10进行了结构分析,确定了化合物7、化合物8、化合物9、化合物10的结构分别为阿魏酸、2-(1-H-吲哚-3-基)丁酸乙酯、阿魏酸正丁酯和2-(1-H-吲哚-3-基)己酸乙酯。化合物1为含有两个羟基的不饱和长链脂肪酸,其分子量为578,分子式为C_(37)H_(70)O_4。化合物2与化合物1属于同一类。化合物5的分子量为306。
Pythium aphanidermatum is a kind of pathogen belongs to Oomycete,which can cause many diseases such as bed rot of many kinds of crops,melons and fruits. The previous results showed that PA1 of P.aphanidermatum,which was isolated from the infected Perennial ryegrass had herbicidal activity.In this study,PA1 and the other 4 isolates that isolated from different hosts were researched detailedly,such as the production,separation and structure identification of the herbicidal substances.
Different melons and fruits were used to isolate the pathogen P. aphanidermatum,and 4 isolates were obtained.Bioassy of the crude toxins produced by the 4 isolates and PA1 was tested by the methods of seed germination and inhibition on weed growth.The results showed that PA1 had the stronger herbicidal activity to Digitaria sanguinalis,and the isolate PAC had stronger activity to Amaranthus retroflexus.
31 and 33 mutant-isolates were obtained by irradiating the mycelia of PA1 and PAC under UV illumination and their herbicidal activity were researched.The results showed that only fewness mutant isolates as PA1-M1and PA1-M4 from PA1 had a increased herbicidal activity,and the activity of other isolates was nealy the same as PA1 or decreased.Different numbers of isolates were obtained from PAC when irradiated by different time,and the herbicidal activity of most of the mutant isolates was increased,and that of the isolate PAC-M2 was stronger to A.retroflexus.
The wheat bran,millet,rice,corn,oat and soybean meal were used as solid media to ferment PA1-M1 and PAC-M2,and the crude toxins were bioassyed and separated using the method of TLC and HPLC.The results showed that the herbicidal activity of the crude toxins from wheat bran was better than the others. HPLC analysis was done to the crude toxins from wheat bran after adding different nutration such as beef extracts,peptone,yeast extracts,and the results showed that the toxin production of that of peptone was highest,so the wheat bran,in which added some peptone was selected as the optimal solid culture for the PA1-M1 and PAC-M2.
PD media was used to ferment PA1 and PAC-M2,and the affection of culturing modes such as shaking and stillness,and pH of PD on the dry weight of mycelia and herbicidal activity of the filtrate were studied.The results showed that the more suitable condition for the producing of mycelia and filtrate were shaking culturing at intial pH5-7.
Herbicidal activity of filrate extracts and mycelia extracts produced by PA1-M1 and PAC-M2 was tested.The results showed that the IC_(50) of filtrate extracts from PA1-M1 to the root and bud of Digitaria sanguinalis was 0.36mg/mL and 0.46mg/mL respectively,while that from PAC-M2 were 0.49mg/mL and 0.36mg/mL respectively.The filtrate extracts were steady to heat and had herbicidal activity to the weeds tested.The IC(50) of mycelia extracts from PA1-M1 to the root and bud of Amaranthus retroflexus was 7.36 mg/mL and 4.09 mg/mL,and that from PAC-M2 was 3.74 mg/mL and 4.76 mg/mL respectively.
The metabolites produced by PA1 in liquid media was researched in detail using the method of solvent extacting,adsorbing by colophony,TLC,Silica Gel Column, Chromatography and HPLC.Compound 1 and compound 2 was obtained from the mycelia extracts.The eluting mode was determined as 50%ethanol,95%ethanol, ethyl acetate and acetone in turn,and the eluting fractions were separated with different methods.In this way,compound 3,compound 4,compound 5 and compound 6 were obtained respectively.The results of HPLC showed that compound 4 was determined as dimethyl 4-nitrophthalate.
Compound 7,compound 8,compound 9 and compound 10 were obtained by using ethyl acetate extrating,TLC,Silica Gel Column Chromatography and HPLC from the solid fermented extration.
IR,NMR,LC-MS were used to identify and analysis the chemical structure of the compounds obtained.The structure of compound 7,8,9 and 10 was ferulic acid, 2-(1H-indol-3-yl)ethyl butyrate,butyl-3-(3-methoxy-4-methylphenyl)acrylate,and 2-(1H-indol-3-yl)ethyl hexanoate,respectively.
Compound 1 was an unsaturated fatty acid with 2 hydroxyls and the molecular weight and molecular formula was 578 and C_(37)H_(70)O_4 respectively.Compound 2 was concluded to be the same type with componud 1,and MS spectrum indicated the molecular weight of compound 5 was 306.
引文
[1]李永泉,庄晓峰.微生物除草剂研究进展[N].农药论坛(第二辑),2000.12.
[2]强胜.杂草学[M].北京:中国农业出版社,2001.1-5.132-163.
[3]夫·夫·察乌思,布·夫·普亚切茨基著.李宝华译.远东应用物理除草机械防除大豆出杂草的前景[J].大豆通报,2002,1:25.
[4]徐汉虹.植物化学保护[M].北京:中国农业出版社.2007.168.
[5]Fischer A J,Ateh C M,Bayer D E,et al.Herbicide-resistant Echinochloa oryzoids and E.Phyllopgon in California,Oryza sativa fields[J].Weed Science,2000,48:225-230.
[6]Vasilakoglou I B,Eleftherohorinos I G,Dhima K V.Propanil-resistant bamyardgrass(Echinochloa crusgalli) biotypes found in Greece[J].Weed Technology,2000,(14):524-529.
[7]马晓渊.农田杂草抗药性的发生为害原因与治理[J].杂草科学,2002,(1):5-9.
[8]苏少泉.稻田杂草对除草剂的抗性及其防治[J].农药,2001,40(7):11-14.
[9]Yasuor H,TenBrook P L,Tjeerdema R S,et al.Responses to clomazone and 5-ketoclomazone by Echinochloa phyllopogon resistant to multiple herbicides in Californian rice fields[J].Pest Management Science.2008,64(10):1031-1039.
[10]赵永根,卞觉时,蔡良华,等.除草剂药害事故的发生与应对策略[J].农药科学与管理,2008,29(8):51-55.
[11]马娟,董金皋.微生物除草剂与生物安全[J].植物保护,2006,32(1):9-12.
[12]董玉霞.灰葡萄孢除草相关基因的克隆与功能分析[D].保定:河北农业大学,2008.
[13]向梅梅,李华平,姜子德,等.微生物除草剂研究现状与展望[J].仲恺农业技术学院学报,2005,18(4):64-69.
[14]吴文君,高希武.生物农药及其应用[M].北京:化学工业出版社.2004.
[15]Duke S O,Dayan F E,Romagni J G,et al.Natural products as sources of herbicides:current status and future trends[J].Weed Research,2000,40:99-110.
[16]强胜.生物除草剂的最新研究进展[J].杂草科学,1997,(4):9-10.
[17]李海涛,王金信,杨合同,等.微生物除草剂的研究现状和应用前景[J].山东科学,2005,18(1):41-46.
[18]陈勇.尖角突脐孢菌作为真菌除草剂防除稗草潜力的研究[D].北京:中国农业科学研究院.2001.
[19]王晓艳.生防菌株SF-193对空心莲子草的防除作用及应用[D].扬州:扬州大学硕士学位论文.2007,8-10
[20]张金林.坪草腐霉病菌除草活性毒素组分的分离鉴定与突变菌株的DDRT差异显示分析[D].保定:河北农业大学,2005.
[21]李扬汉.张宗俭,王建书,等.有关真菌除草剂研究的进展[J].生物防治通报,1994,10(1):35-39.
[22]吴保峰,刁治民,熊亚.微生物除草剂的研究现状及应用前景[J].青海草业,2004,13(2): 34-39.
[23]赵永贵,郭超群,韩恒芬.微生物农药研究的现状及展望[J].河南农业大学学报,1995,29(3):304-310.
[24]强胜.生物除草剂的研究概况[J].杂草科学,1996(2):11-14.
[25]Boyetchko S M.Principles of biological weed control with microorganism[J].Hort Science,1997,32(2):201-205.
[26]雷松僧.谈生物除草剂[J].农药,1991,30(6):30-33.
[27]顾成波,赵长山.微生物及其天然产物防治杂草的发展及展望[J].农药科学与管理,2003,24(2):19-21.
[28]De Jone M D,Scheepens P C,Zadoks J C.Risk analysis for biological control:a dutch case study in biocont rol of Prunus serotina by t he fungus Chondrostereum purpureum[J].Plant Disease,1990,74:189-194.
[29]http://www.bfs.wur.nl/NR/rdonlyres/E158C 176-C828-44D7-B278-89B36EE4CAA9/10478/Bioh erbicidearticleFinalVersionMeindert1.doc
[30]http://www.bd-st.gov.cn/ccj.doc
[31]Saxena S,Pandey A K.Evaluation of an indigenous isolate of Alternaria alternata(LC#508) for use as a mycoherbicide for Lantana camara L[J].Crop Protection.2002.21(1):71-73.
[32]李铷,董锦艳,向梅梅.微生物源除草剂的研究、应用现状及展望[J],杂草科学,2004(4):1-7.
[33]罗小勇.微生物除草剂的发展现状与展望[J].甘肃科学学报,1995,3:54-60.
[34]黄世文.余柳青,Watson,A K.利用稗草病原菌开发微生物除草剂的研究.微生物农药及其产业化[M].北京:科学出版社,2000:260-266.
[35]Kempenaar C.Studies on biological control of chenoopodium album by Ascochyta caulina [D].Wageningen,The Netherlands:Thesis University of Agriculture,1995.
[36]Kilian M,et al.Biological Herbicides-Mycoherbicides[A].In:Fungal iotechnology[M].Chapman& Hall,Weinheim,1997,51-64.(seereference)
[37]Charudattan,R.The mycoherbicide approach with plant pathogens[M].New York:Academic Press,1991.331-394.
[38]Trujillo E E,Latterell F M.Colletotrichum glocosporioides,a possible biological control agent for Clidemiahirta in Hawaiian forests[J].Plant Disease,1986,70:974-976.
[39]曾青,强胜.波斯婆婆纳生防菌株QZ-97a的分离鉴定与致病性研究[J].南京农业大学学报,2000,23(3):21-24.
[40]强胜.胶孢炭疽菌波斯婆婆纳专化型及其侵染过程[J].植物保护学报,2001,28(3):279-284.
[41]YangY K,Kim S O,Chung H S,et al.Use of Colletotrichum graminicola KA001 tocontrol barnyard grass[J].Plant Disease,2000,84(1):55-59.
[42]Kilgore E M,Sugiyama L S,Barreto R.Prospective biological control ofmiconia calvescens in HAWA I'I with a non-indigenous fungus Colletotrichum gloeosporides(Penz.) Sacc.f.sp.miconiae[R].Proceedings of the Firsr Regonal Conference on M iconia Control,1997.
[43]Barreto R,Charudattan R,Pomella A,et al.Biological control of neotropical aquatic weeds with fungi[J].Crop Protection,2000,19:697-703.
[44]Barreto R W,TorresA N L.Nimbya alternantherae and Cercospora alternantherae:two new records of fungal pathogens on Alternanthera philoxeroides(allegatorweed) in Brazil [J].Australasian Plant Pathology,1999,28:103-107.
[45]Anon.Discovery and development of plant pathogens for biological control of weeds[A].In:Reg Res Proj SRCS 8801(S-136 ).Plant Pathology Department of University of Florida.Gainesrille,1989.44.
[46]Kadir J B,Charudattan R,Stall W M,et al.Field efficacy ofDactylaria higginsii as a bioherbicide for the control ofpurple nutsedge(Cyperusrotundus)[J].Weed technology,2000,14(1):1-6.
[47]陈勇,倪汉文.中国稗草病原真菌对稗草及水稻的致病性[J].中国生物防治,1999,15(2):73-76.
[48]王拱辰,叶琪铭.镰刀菌在生物防治中的作用[J].生物防治通报,1990,6(2):80-84.
[49]王之樾,朱广冀,马德成.应用镰刀菌防治瓜列当[J].生物防治通报,1985,1(1):24-26.
[50]Amsellem Z,Kleifeld Y,Kerenyi Z,et al.Isolation,identification,and Activity of mycoherbicidal pathogens from Juvenile Broomrape[J].Biological Control,2001,21:274-284.
[51]Thomas H,Sauerborn J,Muller-Stover D,et al.Fusarium oxysporum f.sp.orthoceras,a potential mycoherbicide,parasitizesseeds of Orobanche Cumana(sunflower broomrape):A cytological study[J].Annals of Botany(London),1999,83:453-458.
[52]付颖,叶非,王常波,等.生物源除草剂研究与使用进展[J].农药,2002,41(5):7-10.
[53]向梅梅,刘任,曾永三.空心莲子草叶斑病菌研究初报[J].吉林农业大学学报,1998,20(增刊):124.
[54]向梅梅,曾永三,刘任,等.莲子草假隔链格孢的寄主范围及对空心莲子草的控制作用[J].植物病理学报.2002,32(3):286-287.
[55]Grace B S,Muller-Scharer H.Biological control of Senecio vulgaris in carrots(Daucus carota)with the rust fungus Puccinia lagenophorae[J].Basic and Applied Ecology,2003,4(4):375-384.
[56]Gupta V P,Kumar V,Mishra R K,et al.Puccinia romagnoliana Marie&Sacc.-a potential bioherbicide agent for biocontrol of purple nutsedge(Cyperus rotundus L.) in mulberry[J].Journal of Phytopathology,2002,150(4-5):263-270.
[57]Peng G,Byer K N,Bailey K L.Pyricularia setariae:a potential bioherbicide agent for control of greenfoxtail(Setaria viridis)[J].Weed Science,2004,52(1):105-114.
[58]El-Sayed W,Kang Z,Hurle K.Infection process of Phomopsis convolvulus as a mycoherbicide for Convolvulus arvensis L[J].Zeitschrift fur Pflanzenkrankheiten und Pflanzenaschutz,2002,109 (2):180-192.
[59]Hofnteister F M,Charudattan R.Psudocercospra nigricans,a pathogen ofsicklepod(Cassia obtusifolia) with biobontrol potential[J].Plant Disease,1987,71:44-46.
[60]Guntli D,St(?)phane B,Ingeborg K,et al.Biological control of hedge bindweed(Calystegia sepium) with Stagonospora convoluvuli strain LA39 in combination with competition from red clover(Triflolium pratense)[J]Biocontrol,1999,15:252-258.
[61]沈寅初,张一斌.生物农药[M].北京:化学工业出版社,2000.
[62]Strobel G,Kenfield D.Phytotoxins as potential herbicides[J].Experientia,1991,47:819-826.
[63]董金皋,李树正.植物病原真菌毒素研究进展(第一卷)[M].北京:中国科学技术出版社,1997:227-232.
[64]Abbas H K,Duke S O.Phytotoxins from plant pathogens as potential herbicides[J].Journal of Toxicology - Toxin Reviews,1995,14:523-543.
[65]Antonio E,Renato C,Adele C,et al.Ascaulitoxin,a phytotoxic bis-aminoacid N-glucoside from Ascochyta caulina[J].Phytochemistry,1998,48(7):1131-1137,1998.
[66]Evidente A,Andolfi A,Vurro M,et al.Trans-4-aminoproline,a phytotoxic metabolite with herbicidal activity produced by Ascochy tacaulina[J].Phytochemistry,2000(53):231 237.
[67]Cutler H G,Jacyno J M,Harwood J S,et al.Botcinolide:a biologically active natural product from Botrytis cinerea[J].Biosci Biotech Biochem,1993,57(11):1980-1982.
[68]John M,Jacyno,John S,et al.Structure and solution-state conformation of botcinolide,a new biologically active metabolite from the fungus Botrytis cinerea[J].Tetrahedron,1994,50(40):11585-11592
[69]Wang H,Dong J,Shang H.An ABAGS-Like metabolite ofBotrytis cinerea isolate BC4 and its inhibitory activity to seed germination of weeds[J].Agricultural Sciences in China,2005,4(11):845-850.
[70]Wang H,Dong J,Shang H.An ABAGE-Like metabolite ofBotrytis cinerea isolate BC4 inhibited the growth of hypocotyls and roots of Amaranthus retroflexus seedlings[J].Agricultural Sciences inChina,2005,4(8):548-588.
[71]Levy L M.Indole alkaloids from a culture of the fungus Aporpium caryae[J].Phytochemistry,2000,54(8):941-943.
[72]Zeng R,Luo S,Shi M,et al.Physiological and biochemical mechanism of allelopathy of secalonic acid on higher plants of Aspergillus japonicus on crops[J].Agronomy Journal,2001,93(1):60-64.
[73]Arnone A,De Gregorio C,Nasini G,et al.Isolation and structure elucidation of Tsugicolines FH,novel furosesquiterpenes,and Tsugicoline I from the fungus Laurilia tsugicola[J].Tetrahedron,1998,54:10199-10204.
[74]刘常林,李玉新,王晓毅,等.结构新颖的微生物源除草剂化合物及其作用特点[J].湖南化工,1999,29(1):4-6.
[75]Nakajiama M,Itoi K,Takamatsu Y,et al.Comexistin:a new fungal metabolite with herbicidal activity[J].Journal of Antibiotics,1989,44:1065-1072.
[76]Stierle A,Upadhyay R,Strobel G,et al.Cyperine,a phytotoxin produced by Ascochyta cypericola,a fungal pathogen of Cyperus rot undus[J].Phytochemistry,1991,30(7):2191-2192.
[77]Arai M,Haneishi T,Kitahara N,et al.Herbicidins A and B,two new antibiotics with herbicidal activity.1.Producing organism and biological activities[J].Journal of Antibiotics,1976,9:863-869.
[78]Isaac B G,Ayer S W,Elliott R C,et al.Herboxidiene:a potent phytotoxic polyketide from Streptomycessp.A7847[J].Journal of Organic Chemistry,1992,57:7220-7226.
[79]Nakajima M,Itoi K,Takamatsu K,et al.Hydantocidin:a new compound with herbicidal activity from Streptomyces hygroscopicus[J].J.Antibiot,1991,44:293-300.
[80]Worthing C R.British Crop Protection Council.The Pesticide Manual,6th Edition[M].Croydon,1979.349.
[81]Gerwick B C,Fields S S,Graupner P R,et al.Pyridazocidin,a new microbial phytotoxin with activity in the mehler reaction[J].Weed Science,1997,45:654-657.
[82]宗兆锋,康振生.植物病理学原理[M].中国农业出版社,2002,46.
[83]刘大群,董金皋.植物病理学导论[M].科学出版社,北京,2007,30.
[84]Shimada A,Takeuchi S,Nakajima A,et al.Phytotoxicity ofindole-3-acetic acid produced by the fungus,Pythium aphanidermatum[J].Bioscience Biotechnology and Biochemistry,1999,64(1):187-189.
[85]陈捷,高洪敏,纪明山,等.玉米茎基腐病菌产生的细胞壁降解酶的致病作用[J].植物病报,199,28(3):221-226.
[86]马娟.灰葡萄孢除草活性菌株的诱变及其相关基因的差异显示[D].保定:河北农业大学,2005.
[87]陈年春.农药生物测定技术[M].北京:北京农业大学出版社,1991.96-217.
[88]Strobel G A,Kenfield D,Bunkers G,et al.Phytotoxins as potential herbicides[J].Experientia,1991,47:819-826.
[89]Mohamed A,Abouzeid,Angela B.et al.Toxicity profiles of potential biocontrol agents of Orobanche ramosa[J],Weed Science,2004,52:326-332.
[90]齐丽宁,杨霞,任志琴.除草剂生物活性测定.天津师大学报(自然科学版)[J],1999,19(4):6-11.
[91]宋小玲,马波,皇甫超河,等.除草剂生物测定方法[J].杂草科学,2004,(3):1-6.
[92]吴声敢,王强,赵学平.稗草对7种除草剂的敏感性及其生物测定方法的研究[J].浙江农业学报,2007,19(1):37-41.
[93]唐伟,孙军德,张翠霞.农用抗生素产生菌种选育的研究进展[J].微生物学杂志,2004,24(4):42-45.
[94]吴焕利,冯贵颖,赵淑艳,等.紫外诱变选育高效微生物絮凝剂产生菌及其应用[J].西北农林科技大学学报(自然科学版),2008,36(8):113-118.
[95]李薇,劳兴珍,郑珩,等.高产谷胱甘肽酵母菌的诱变选育以及发酵条件的初步研究[J].药物生物技术,2008,15(1):48-50.
[96]俞俊堂,唐孝宣.生物工艺学(上册)[M].上海:华东理工大学出版社,1991.82-84.
[97]Werner E G,Anette R,Johannes M,et al.Regulation of enzymes involved in anthocyanin biosynthesis in carrot cell cultures in response to treatment with ultraviolet light and fungal elicitors[J].Planta,1998,204:490-498.
[98]朱芬,陈军,师亮.灵芝三萜高产菌株原生质体紫外诱变选育[J].南京农业大学学报 2008,31(1):37-41.
[99]黄乾明,杨婉身,陈华萍.粗毛栓菌诱变菌株SAH-12漆酶的分离纯化及酶学性质研究[J],菌物学报,2007,26(4):539-548.
[100]庄惠如,陈必链,陈荣,等.雨生红球藻的紫外、激光复合诱变育种[J].激光生物学报,2001,10(2):135-139.
[101]黄永春,曹仁林,彭祎.紫外诱变吸水链霉菌选育高产农用抗生素菌株[J].安徽农业科学,2008,36(16):6847-6848.
[102]王惠.灰葡萄孢生物活性代谢产物的研究.西北农林科技大学[D].中国 杨凌.2004
[103]万佐玺,强胜,徐尚成.链格孢菌的产毒培养条件及其毒素的致病范围[J].中国生物防治,2001,17(1):10-15.
[104]李荣金,强胜.百目草链格孢菌粗毒素的生产、提取及稳定性的研究[J].中国生物工程杂志,2006,26(8):67-71.
[105]Boyette C D,Quimby P C Jr,et al.Progress in the production,formulation and application of mycoherbicides[M].In:Tebeest,D.V.ed.Microbial Control of Weeds.Chapman and Hall,1991,209-222.
[106]张金林,庞民好,刘颖超,等.坪草腐霉病菌毒素产生除草活性物质的条件优化[J].河北农业大学学报,2005(28)4:84-88.
[107]韩庆莉.灰葡萄孢菌不同发酵方式次生代谢产物的生物活性[J],西南林学院学报,2006,25(4):5-8.
[108]李国友.五种毛壳霉属和曲霉属真菌代谢产物及细胞毒活性[D].中国科学院研究生院博士学位论文,成都,2007.
[109]陶玉贵.生物农药固态发酵条件的研究[J].安徽师范大学学报(自然科学版),2001,24(1):31-33.
[110]张正波.除草微生物禾长蠕孢的菌种改良与制剂的研究.中国农业科学院博士学位论文.中国水稻所.2007.
[111]耿锐梅,傅扬,张文明,等.麦根腐平脐蠕孢和薏苡平脐蠕孢防治稻田稗草的生物活性和安仝性[J].中国水稻科学,2008,22,(3):307-312.
[112]Junko O,Kenji M.Production of the phytotoxic metabolite.Ferricrocin,by the fungus Colletotrichum gloeosporioides[J].Bioscience Biotechnology and Biochemistry,1995,59(1):113-114.
[113]Evidente A,Lanzetta R,Capasso R,et al.Putaminoxins B and C from Phoma putaminum[J].Phytochemistry,1997,144(6):1041-1045.
[114]Sugawara R,Strobel G,Fisher L E,et al.Bipolaroxin,a selective phytotoxin produced by Bipolaris cynodontis[J],Proceedings of the National Academy of Sciences.U.S.A,1985,82:8291-8294.
[115]Venkatasubbaiah P,Baudoin ABA,Chiiton W S.Leaf spot of hemp dogbane caused by Stagonospora apocyni,and its phytotoxins[J].Journal of Phytopathogy,1992,135:309-316.
[116]Venkatasubbaiah P,Chilton W S.Phytotoxins of Ascochyta hyalosopora,causal agent of lambsqua-rters leaf spot[J].Journal of Natural Products.1992.55:461-467.
[117]王明旭,罗宽.稗叶枯菌及其毒素的研究[J].湖南农学院学报,1991,17(1):34-41.
[118]张金林,董金皋,樊慕贞,等.葱紫斑病菌毒素的纯化及除草活性研究[J].植物保护学报,2000,27(3):285-28.
[119]张金林,董金皋,樊慕贞,等.葱叶枯病菌毒素的分离与除草活性研究[J].农药学学报,2001,3(2):60-66.
[120]姜述君,范文艳,鞠世杰,等.狭卵链格孢菌株AAEC05-3及其毒素对稗草的致病性[J].植物保护学报,2007,34(3):282-287.
[121]王惠,张凤云,董金皋,等.灰葡萄孢及其毒素的生物活性测定[J].西北农林科技大学学报(自然科学版),2003,31(4):119-122.
[122]汪茂田,谢培山,王忠东,等.天然有机化合物提取分离与结构鉴定[M].机械工业出版社.2004,100-105,133,75.
[123]屠式玫.SIP系列大孔吸附树脂的性能及应用[J].医药工业,1987,18(4):177.
[124]刘峰群,肖小河,史成和,等.大孔吸附树脂在药物分离中的研究应用[J].中国医院药学杂志2000,20(3):166-167.
[125]张利辉,王毅靖,张金林,董金皋.树脂吸附法分离瓜果腐霉毒素除草活性物质[J].微生物学通报,2008,35(9):1505-1509.
[126]张利辉,张洋,司贺龙,董金皋.瓜果腐霉毒素除草活性物质的初步分离[J].河北农业大学学报2008,31(5):71-74.
[127]苏少泉.生物除草剂的研究与开发[J].农药,2004,43(3):97-100.
[128]Pachlatko J P.Natural products in crop protection.Second International Electronic Conference on Synthetic Organic Chemistry(ECSOC-2).ECSOC,Basel,Switzerland.[Online.]1998.http://www.ecsoc2.hec.ru/d1001/d1001.htm.(See Reference)
[129]赵善欢.植物化学保护(第三版)[M].北京:中国农业出版社,2000.301-312.
[130]高菊芳.生物农药的作用、应用与功效[J].世界农药,2001,23(1):1-6.
[131]宁永成.有机化合物结构鉴定与有机波谱学[M].科学出版社,北京.2000,485-499.
[132]李拥军,陈实,李春远,等.薇甘菊化感活性成分的分离与鉴定[J].华南农业大学学报,2008,29(4):26-30.
[133]薛枫,欧仕益,汪勇.双酶法降解细胞壁物质制备低聚木糖和阿魏酸研究进展[J].粮食 与饲料工业,2005,5:28-30.
[134]孙玉琴,陈中坚,韦美丽等.阿魏酸对三七化感作用的初步研究.特产研究,2008,(2):39-41.
[135]Faulds C B,Aliwan F O.Chemical and thermal stability of ferulic acid(feruloyl) esterases from Aspergillus[J].Progress in Biotechnology,1998,15:41-46.
[136]张璟.黑曲霉液体发酵法产酶制备阿魏酸和低聚糖的研究[D].暨南大学.2004.
[137]Ahmed Eid Fazary,Yi-Hsu Ju.Production,partial purification and characterization of feruloyl esterase by Aspergillus awamori in submerged fermentation[J].Biotechnology Journal,2008,3(9-10):1264-1275.
[138]Ou S Y,Kwok K C.Review ferulic acid:pharmaceutical functions,preparation and applications in foods[J].Journal of the Science of Food and Agriculture,2004,84:1261-1269.
[139]Topakas E,Stamatis H.Purification and characterization of a feruloyl esterase from Fusarium oxysporum catalyzing esterification of phenolic acids in ternary water organic solventixtures[J].Journal of Biotechnology,2003,102:33-44.
[140]Topakas E,Kalogeris E.Production and partial characterisation of feruloyi esterase by Porotrichum thermophile in solidstate fermentation[J].Process Biochemistry,2003,38:1539-1543.
[2]强胜.杂草学[M].北京:中国农业出版社,2001.1-5.132-163.
[3]夫·夫·察乌思,布·夫·普亚切茨基著.李宝华译.远东应用物理除草机械防除大豆出杂草的前景[J].大豆通报,2002,1:25.
[4]徐汉虹.植物化学保护[M].北京:中国农业出版社.2007.168.
[5]Fischer A J,Ateh C M,Bayer D E,et al.Herbicide-resistant Echinochloa oryzoids and E.Phyllopgon in California,Oryza sativa fields[J].Weed Science,2000,48:225-230.
[6]Vasilakoglou I B,Eleftherohorinos I G,Dhima K V.Propanil-resistant bamyardgrass(Echinochloa crusgalli) biotypes found in Greece[J].Weed Technology,2000,(14):524-529.
[7]马晓渊.农田杂草抗药性的发生为害原因与治理[J].杂草科学,2002,(1):5-9.
[8]苏少泉.稻田杂草对除草剂的抗性及其防治[J].农药,2001,40(7):11-14.
[9]Yasuor H,TenBrook P L,Tjeerdema R S,et al.Responses to clomazone and 5-ketoclomazone by Echinochloa phyllopogon resistant to multiple herbicides in Californian rice fields[J].Pest Management Science.2008,64(10):1031-1039.
[10]赵永根,卞觉时,蔡良华,等.除草剂药害事故的发生与应对策略[J].农药科学与管理,2008,29(8):51-55.
[11]马娟,董金皋.微生物除草剂与生物安全[J].植物保护,2006,32(1):9-12.
[12]董玉霞.灰葡萄孢除草相关基因的克隆与功能分析[D].保定:河北农业大学,2008.
[13]向梅梅,李华平,姜子德,等.微生物除草剂研究现状与展望[J].仲恺农业技术学院学报,2005,18(4):64-69.
[14]吴文君,高希武.生物农药及其应用[M].北京:化学工业出版社.2004.
[15]Duke S O,Dayan F E,Romagni J G,et al.Natural products as sources of herbicides:current status and future trends[J].Weed Research,2000,40:99-110.
[16]强胜.生物除草剂的最新研究进展[J].杂草科学,1997,(4):9-10.
[17]李海涛,王金信,杨合同,等.微生物除草剂的研究现状和应用前景[J].山东科学,2005,18(1):41-46.
[18]陈勇.尖角突脐孢菌作为真菌除草剂防除稗草潜力的研究[D].北京:中国农业科学研究院.2001.
[19]王晓艳.生防菌株SF-193对空心莲子草的防除作用及应用[D].扬州:扬州大学硕士学位论文.2007,8-10
[20]张金林.坪草腐霉病菌除草活性毒素组分的分离鉴定与突变菌株的DDRT差异显示分析[D].保定:河北农业大学,2005.
[21]李扬汉.张宗俭,王建书,等.有关真菌除草剂研究的进展[J].生物防治通报,1994,10(1):35-39.
[22]吴保峰,刁治民,熊亚.微生物除草剂的研究现状及应用前景[J].青海草业,2004,13(2): 34-39.
[23]赵永贵,郭超群,韩恒芬.微生物农药研究的现状及展望[J].河南农业大学学报,1995,29(3):304-310.
[24]强胜.生物除草剂的研究概况[J].杂草科学,1996(2):11-14.
[25]Boyetchko S M.Principles of biological weed control with microorganism[J].Hort Science,1997,32(2):201-205.
[26]雷松僧.谈生物除草剂[J].农药,1991,30(6):30-33.
[27]顾成波,赵长山.微生物及其天然产物防治杂草的发展及展望[J].农药科学与管理,2003,24(2):19-21.
[28]De Jone M D,Scheepens P C,Zadoks J C.Risk analysis for biological control:a dutch case study in biocont rol of Prunus serotina by t he fungus Chondrostereum purpureum[J].Plant Disease,1990,74:189-194.
[29]http://www.bfs.wur.nl/NR/rdonlyres/E158C 176-C828-44D7-B278-89B36EE4CAA9/10478/Bioh erbicidearticleFinalVersionMeindert1.doc
[30]http://www.bd-st.gov.cn/ccj.doc
[31]Saxena S,Pandey A K.Evaluation of an indigenous isolate of Alternaria alternata(LC#508) for use as a mycoherbicide for Lantana camara L[J].Crop Protection.2002.21(1):71-73.
[32]李铷,董锦艳,向梅梅.微生物源除草剂的研究、应用现状及展望[J],杂草科学,2004(4):1-7.
[33]罗小勇.微生物除草剂的发展现状与展望[J].甘肃科学学报,1995,3:54-60.
[34]黄世文.余柳青,Watson,A K.利用稗草病原菌开发微生物除草剂的研究.微生物农药及其产业化[M].北京:科学出版社,2000:260-266.
[35]Kempenaar C.Studies on biological control of chenoopodium album by Ascochyta caulina [D].Wageningen,The Netherlands:Thesis University of Agriculture,1995.
[36]Kilian M,et al.Biological Herbicides-Mycoherbicides[A].In:Fungal iotechnology[M].Chapman& Hall,Weinheim,1997,51-64.(seereference)
[37]Charudattan,R.The mycoherbicide approach with plant pathogens[M].New York:Academic Press,1991.331-394.
[38]Trujillo E E,Latterell F M.Colletotrichum glocosporioides,a possible biological control agent for Clidemiahirta in Hawaiian forests[J].Plant Disease,1986,70:974-976.
[39]曾青,强胜.波斯婆婆纳生防菌株QZ-97a的分离鉴定与致病性研究[J].南京农业大学学报,2000,23(3):21-24.
[40]强胜.胶孢炭疽菌波斯婆婆纳专化型及其侵染过程[J].植物保护学报,2001,28(3):279-284.
[41]YangY K,Kim S O,Chung H S,et al.Use of Colletotrichum graminicola KA001 tocontrol barnyard grass[J].Plant Disease,2000,84(1):55-59.
[42]Kilgore E M,Sugiyama L S,Barreto R.Prospective biological control ofmiconia calvescens in HAWA I'I with a non-indigenous fungus Colletotrichum gloeosporides(Penz.) Sacc.f.sp.miconiae[R].Proceedings of the Firsr Regonal Conference on M iconia Control,1997.
[43]Barreto R,Charudattan R,Pomella A,et al.Biological control of neotropical aquatic weeds with fungi[J].Crop Protection,2000,19:697-703.
[44]Barreto R W,TorresA N L.Nimbya alternantherae and Cercospora alternantherae:two new records of fungal pathogens on Alternanthera philoxeroides(allegatorweed) in Brazil [J].Australasian Plant Pathology,1999,28:103-107.
[45]Anon.Discovery and development of plant pathogens for biological control of weeds[A].In:Reg Res Proj SRCS 8801(S-136 ).Plant Pathology Department of University of Florida.Gainesrille,1989.44.
[46]Kadir J B,Charudattan R,Stall W M,et al.Field efficacy ofDactylaria higginsii as a bioherbicide for the control ofpurple nutsedge(Cyperusrotundus)[J].Weed technology,2000,14(1):1-6.
[47]陈勇,倪汉文.中国稗草病原真菌对稗草及水稻的致病性[J].中国生物防治,1999,15(2):73-76.
[48]王拱辰,叶琪铭.镰刀菌在生物防治中的作用[J].生物防治通报,1990,6(2):80-84.
[49]王之樾,朱广冀,马德成.应用镰刀菌防治瓜列当[J].生物防治通报,1985,1(1):24-26.
[50]Amsellem Z,Kleifeld Y,Kerenyi Z,et al.Isolation,identification,and Activity of mycoherbicidal pathogens from Juvenile Broomrape[J].Biological Control,2001,21:274-284.
[51]Thomas H,Sauerborn J,Muller-Stover D,et al.Fusarium oxysporum f.sp.orthoceras,a potential mycoherbicide,parasitizesseeds of Orobanche Cumana(sunflower broomrape):A cytological study[J].Annals of Botany(London),1999,83:453-458.
[52]付颖,叶非,王常波,等.生物源除草剂研究与使用进展[J].农药,2002,41(5):7-10.
[53]向梅梅,刘任,曾永三.空心莲子草叶斑病菌研究初报[J].吉林农业大学学报,1998,20(增刊):124.
[54]向梅梅,曾永三,刘任,等.莲子草假隔链格孢的寄主范围及对空心莲子草的控制作用[J].植物病理学报.2002,32(3):286-287.
[55]Grace B S,Muller-Scharer H.Biological control of Senecio vulgaris in carrots(Daucus carota)with the rust fungus Puccinia lagenophorae[J].Basic and Applied Ecology,2003,4(4):375-384.
[56]Gupta V P,Kumar V,Mishra R K,et al.Puccinia romagnoliana Marie&Sacc.-a potential bioherbicide agent for biocontrol of purple nutsedge(Cyperus rotundus L.) in mulberry[J].Journal of Phytopathology,2002,150(4-5):263-270.
[57]Peng G,Byer K N,Bailey K L.Pyricularia setariae:a potential bioherbicide agent for control of greenfoxtail(Setaria viridis)[J].Weed Science,2004,52(1):105-114.
[58]El-Sayed W,Kang Z,Hurle K.Infection process of Phomopsis convolvulus as a mycoherbicide for Convolvulus arvensis L[J].Zeitschrift fur Pflanzenkrankheiten und Pflanzenaschutz,2002,109 (2):180-192.
[59]Hofnteister F M,Charudattan R.Psudocercospra nigricans,a pathogen ofsicklepod(Cassia obtusifolia) with biobontrol potential[J].Plant Disease,1987,71:44-46.
[60]Guntli D,St(?)phane B,Ingeborg K,et al.Biological control of hedge bindweed(Calystegia sepium) with Stagonospora convoluvuli strain LA39 in combination with competition from red clover(Triflolium pratense)[J]Biocontrol,1999,15:252-258.
[61]沈寅初,张一斌.生物农药[M].北京:化学工业出版社,2000.
[62]Strobel G,Kenfield D.Phytotoxins as potential herbicides[J].Experientia,1991,47:819-826.
[63]董金皋,李树正.植物病原真菌毒素研究进展(第一卷)[M].北京:中国科学技术出版社,1997:227-232.
[64]Abbas H K,Duke S O.Phytotoxins from plant pathogens as potential herbicides[J].Journal of Toxicology - Toxin Reviews,1995,14:523-543.
[65]Antonio E,Renato C,Adele C,et al.Ascaulitoxin,a phytotoxic bis-aminoacid N-glucoside from Ascochyta caulina[J].Phytochemistry,1998,48(7):1131-1137,1998.
[66]Evidente A,Andolfi A,Vurro M,et al.Trans-4-aminoproline,a phytotoxic metabolite with herbicidal activity produced by Ascochy tacaulina[J].Phytochemistry,2000(53):231 237.
[67]Cutler H G,Jacyno J M,Harwood J S,et al.Botcinolide:a biologically active natural product from Botrytis cinerea[J].Biosci Biotech Biochem,1993,57(11):1980-1982.
[68]John M,Jacyno,John S,et al.Structure and solution-state conformation of botcinolide,a new biologically active metabolite from the fungus Botrytis cinerea[J].Tetrahedron,1994,50(40):11585-11592
[69]Wang H,Dong J,Shang H.An ABAGS-Like metabolite ofBotrytis cinerea isolate BC4 and its inhibitory activity to seed germination of weeds[J].Agricultural Sciences in China,2005,4(11):845-850.
[70]Wang H,Dong J,Shang H.An ABAGE-Like metabolite ofBotrytis cinerea isolate BC4 inhibited the growth of hypocotyls and roots of Amaranthus retroflexus seedlings[J].Agricultural Sciences inChina,2005,4(8):548-588.
[71]Levy L M.Indole alkaloids from a culture of the fungus Aporpium caryae[J].Phytochemistry,2000,54(8):941-943.
[72]Zeng R,Luo S,Shi M,et al.Physiological and biochemical mechanism of allelopathy of secalonic acid on higher plants of Aspergillus japonicus on crops[J].Agronomy Journal,2001,93(1):60-64.
[73]Arnone A,De Gregorio C,Nasini G,et al.Isolation and structure elucidation of Tsugicolines FH,novel furosesquiterpenes,and Tsugicoline I from the fungus Laurilia tsugicola[J].Tetrahedron,1998,54:10199-10204.
[74]刘常林,李玉新,王晓毅,等.结构新颖的微生物源除草剂化合物及其作用特点[J].湖南化工,1999,29(1):4-6.
[75]Nakajiama M,Itoi K,Takamatsu Y,et al.Comexistin:a new fungal metabolite with herbicidal activity[J].Journal of Antibiotics,1989,44:1065-1072.
[76]Stierle A,Upadhyay R,Strobel G,et al.Cyperine,a phytotoxin produced by Ascochyta cypericola,a fungal pathogen of Cyperus rot undus[J].Phytochemistry,1991,30(7):2191-2192.
[77]Arai M,Haneishi T,Kitahara N,et al.Herbicidins A and B,two new antibiotics with herbicidal activity.1.Producing organism and biological activities[J].Journal of Antibiotics,1976,9:863-869.
[78]Isaac B G,Ayer S W,Elliott R C,et al.Herboxidiene:a potent phytotoxic polyketide from Streptomycessp.A7847[J].Journal of Organic Chemistry,1992,57:7220-7226.
[79]Nakajima M,Itoi K,Takamatsu K,et al.Hydantocidin:a new compound with herbicidal activity from Streptomyces hygroscopicus[J].J.Antibiot,1991,44:293-300.
[80]Worthing C R.British Crop Protection Council.The Pesticide Manual,6th Edition[M].Croydon,1979.349.
[81]Gerwick B C,Fields S S,Graupner P R,et al.Pyridazocidin,a new microbial phytotoxin with activity in the mehler reaction[J].Weed Science,1997,45:654-657.
[82]宗兆锋,康振生.植物病理学原理[M].中国农业出版社,2002,46.
[83]刘大群,董金皋.植物病理学导论[M].科学出版社,北京,2007,30.
[84]Shimada A,Takeuchi S,Nakajima A,et al.Phytotoxicity ofindole-3-acetic acid produced by the fungus,Pythium aphanidermatum[J].Bioscience Biotechnology and Biochemistry,1999,64(1):187-189.
[85]陈捷,高洪敏,纪明山,等.玉米茎基腐病菌产生的细胞壁降解酶的致病作用[J].植物病报,199,28(3):221-226.
[86]马娟.灰葡萄孢除草活性菌株的诱变及其相关基因的差异显示[D].保定:河北农业大学,2005.
[87]陈年春.农药生物测定技术[M].北京:北京农业大学出版社,1991.96-217.
[88]Strobel G A,Kenfield D,Bunkers G,et al.Phytotoxins as potential herbicides[J].Experientia,1991,47:819-826.
[89]Mohamed A,Abouzeid,Angela B.et al.Toxicity profiles of potential biocontrol agents of Orobanche ramosa[J],Weed Science,2004,52:326-332.
[90]齐丽宁,杨霞,任志琴.除草剂生物活性测定.天津师大学报(自然科学版)[J],1999,19(4):6-11.
[91]宋小玲,马波,皇甫超河,等.除草剂生物测定方法[J].杂草科学,2004,(3):1-6.
[92]吴声敢,王强,赵学平.稗草对7种除草剂的敏感性及其生物测定方法的研究[J].浙江农业学报,2007,19(1):37-41.
[93]唐伟,孙军德,张翠霞.农用抗生素产生菌种选育的研究进展[J].微生物学杂志,2004,24(4):42-45.
[94]吴焕利,冯贵颖,赵淑艳,等.紫外诱变选育高效微生物絮凝剂产生菌及其应用[J].西北农林科技大学学报(自然科学版),2008,36(8):113-118.
[95]李薇,劳兴珍,郑珩,等.高产谷胱甘肽酵母菌的诱变选育以及发酵条件的初步研究[J].药物生物技术,2008,15(1):48-50.
[96]俞俊堂,唐孝宣.生物工艺学(上册)[M].上海:华东理工大学出版社,1991.82-84.
[97]Werner E G,Anette R,Johannes M,et al.Regulation of enzymes involved in anthocyanin biosynthesis in carrot cell cultures in response to treatment with ultraviolet light and fungal elicitors[J].Planta,1998,204:490-498.
[98]朱芬,陈军,师亮.灵芝三萜高产菌株原生质体紫外诱变选育[J].南京农业大学学报 2008,31(1):37-41.
[99]黄乾明,杨婉身,陈华萍.粗毛栓菌诱变菌株SAH-12漆酶的分离纯化及酶学性质研究[J],菌物学报,2007,26(4):539-548.
[100]庄惠如,陈必链,陈荣,等.雨生红球藻的紫外、激光复合诱变育种[J].激光生物学报,2001,10(2):135-139.
[101]黄永春,曹仁林,彭祎.紫外诱变吸水链霉菌选育高产农用抗生素菌株[J].安徽农业科学,2008,36(16):6847-6848.
[102]王惠.灰葡萄孢生物活性代谢产物的研究.西北农林科技大学[D].中国 杨凌.2004
[103]万佐玺,强胜,徐尚成.链格孢菌的产毒培养条件及其毒素的致病范围[J].中国生物防治,2001,17(1):10-15.
[104]李荣金,强胜.百目草链格孢菌粗毒素的生产、提取及稳定性的研究[J].中国生物工程杂志,2006,26(8):67-71.
[105]Boyette C D,Quimby P C Jr,et al.Progress in the production,formulation and application of mycoherbicides[M].In:Tebeest,D.V.ed.Microbial Control of Weeds.Chapman and Hall,1991,209-222.
[106]张金林,庞民好,刘颖超,等.坪草腐霉病菌毒素产生除草活性物质的条件优化[J].河北农业大学学报,2005(28)4:84-88.
[107]韩庆莉.灰葡萄孢菌不同发酵方式次生代谢产物的生物活性[J],西南林学院学报,2006,25(4):5-8.
[108]李国友.五种毛壳霉属和曲霉属真菌代谢产物及细胞毒活性[D].中国科学院研究生院博士学位论文,成都,2007.
[109]陶玉贵.生物农药固态发酵条件的研究[J].安徽师范大学学报(自然科学版),2001,24(1):31-33.
[110]张正波.除草微生物禾长蠕孢的菌种改良与制剂的研究.中国农业科学院博士学位论文.中国水稻所.2007.
[111]耿锐梅,傅扬,张文明,等.麦根腐平脐蠕孢和薏苡平脐蠕孢防治稻田稗草的生物活性和安仝性[J].中国水稻科学,2008,22,(3):307-312.
[112]Junko O,Kenji M.Production of the phytotoxic metabolite.Ferricrocin,by the fungus Colletotrichum gloeosporioides[J].Bioscience Biotechnology and Biochemistry,1995,59(1):113-114.
[113]Evidente A,Lanzetta R,Capasso R,et al.Putaminoxins B and C from Phoma putaminum[J].Phytochemistry,1997,144(6):1041-1045.
[114]Sugawara R,Strobel G,Fisher L E,et al.Bipolaroxin,a selective phytotoxin produced by Bipolaris cynodontis[J],Proceedings of the National Academy of Sciences.U.S.A,1985,82:8291-8294.
[115]Venkatasubbaiah P,Baudoin ABA,Chiiton W S.Leaf spot of hemp dogbane caused by Stagonospora apocyni,and its phytotoxins[J].Journal of Phytopathogy,1992,135:309-316.
[116]Venkatasubbaiah P,Chilton W S.Phytotoxins of Ascochyta hyalosopora,causal agent of lambsqua-rters leaf spot[J].Journal of Natural Products.1992.55:461-467.
[117]王明旭,罗宽.稗叶枯菌及其毒素的研究[J].湖南农学院学报,1991,17(1):34-41.
[118]张金林,董金皋,樊慕贞,等.葱紫斑病菌毒素的纯化及除草活性研究[J].植物保护学报,2000,27(3):285-28.
[119]张金林,董金皋,樊慕贞,等.葱叶枯病菌毒素的分离与除草活性研究[J].农药学学报,2001,3(2):60-66.
[120]姜述君,范文艳,鞠世杰,等.狭卵链格孢菌株AAEC05-3及其毒素对稗草的致病性[J].植物保护学报,2007,34(3):282-287.
[121]王惠,张凤云,董金皋,等.灰葡萄孢及其毒素的生物活性测定[J].西北农林科技大学学报(自然科学版),2003,31(4):119-122.
[122]汪茂田,谢培山,王忠东,等.天然有机化合物提取分离与结构鉴定[M].机械工业出版社.2004,100-105,133,75.
[123]屠式玫.SIP系列大孔吸附树脂的性能及应用[J].医药工业,1987,18(4):177.
[124]刘峰群,肖小河,史成和,等.大孔吸附树脂在药物分离中的研究应用[J].中国医院药学杂志2000,20(3):166-167.
[125]张利辉,王毅靖,张金林,董金皋.树脂吸附法分离瓜果腐霉毒素除草活性物质[J].微生物学通报,2008,35(9):1505-1509.
[126]张利辉,张洋,司贺龙,董金皋.瓜果腐霉毒素除草活性物质的初步分离[J].河北农业大学学报2008,31(5):71-74.
[127]苏少泉.生物除草剂的研究与开发[J].农药,2004,43(3):97-100.
[128]Pachlatko J P.Natural products in crop protection.Second International Electronic Conference on Synthetic Organic Chemistry(ECSOC-2).ECSOC,Basel,Switzerland.[Online.]1998.http://www.ecsoc2.hec.ru/d1001/d1001.htm.(See Reference)
[129]赵善欢.植物化学保护(第三版)[M].北京:中国农业出版社,2000.301-312.
[130]高菊芳.生物农药的作用、应用与功效[J].世界农药,2001,23(1):1-6.
[131]宁永成.有机化合物结构鉴定与有机波谱学[M].科学出版社,北京.2000,485-499.
[132]李拥军,陈实,李春远,等.薇甘菊化感活性成分的分离与鉴定[J].华南农业大学学报,2008,29(4):26-30.
[133]薛枫,欧仕益,汪勇.双酶法降解细胞壁物质制备低聚木糖和阿魏酸研究进展[J].粮食 与饲料工业,2005,5:28-30.
[134]孙玉琴,陈中坚,韦美丽等.阿魏酸对三七化感作用的初步研究.特产研究,2008,(2):39-41.
[135]Faulds C B,Aliwan F O.Chemical and thermal stability of ferulic acid(feruloyl) esterases from Aspergillus[J].Progress in Biotechnology,1998,15:41-46.
[136]张璟.黑曲霉液体发酵法产酶制备阿魏酸和低聚糖的研究[D].暨南大学.2004.
[137]Ahmed Eid Fazary,Yi-Hsu Ju.Production,partial purification and characterization of feruloyl esterase by Aspergillus awamori in submerged fermentation[J].Biotechnology Journal,2008,3(9-10):1264-1275.
[138]Ou S Y,Kwok K C.Review ferulic acid:pharmaceutical functions,preparation and applications in foods[J].Journal of the Science of Food and Agriculture,2004,84:1261-1269.
[139]Topakas E,Stamatis H.Purification and characterization of a feruloyl esterase from Fusarium oxysporum catalyzing esterification of phenolic acids in ternary water organic solventixtures[J].Journal of Biotechnology,2003,102:33-44.
[140]Topakas E,Kalogeris E.Production and partial characterisation of feruloyi esterase by Porotrichum thermophile in solidstate fermentation[J].Process Biochemistry,2003,38:1539-1543.
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