经轻胶囊对大鼠抑郁的干预机制及性激素对大鼠脑干TPH_2基因表达的影响
|
摘要
目的:分析性激素异常与精神情绪障碍之间的内在联系,用高剂量雌激素造成大鼠抑郁模型,探讨中药经轻胶囊的干预机制;分别用雌激素、孕激素、雄激素刺激雌鼠、雄鼠,进一步探索性激素对大鼠行为及脑干5-HT、TPH_2基因表达的影响,分析性激素与抑郁的相关性。方法:选择3—4月龄雌性和雄性SD大鼠,分别对每只雌鼠进行阴道脱落细胞涂片观察,逐只建立动情周期档案。在第一个实验中选择符合条件的大鼠,随机分为8组:雌鼠空白组、雄鼠空白组、雌鼠雌激素模型组、雄鼠雌激素模型组、利血平模型组、雌鼠雌激素模型+中药组、雄鼠雌激素模型+中药组、利血平模型组+中药组。选择大鼠的动情期,每日上午皮下注射苯甲酸雌二醇注射液,连续3天。造成实验大鼠体内高雌激素状态,按不同组别给予经轻胶囊干预,观察大鼠行为学、血清雌二醇、孕酮、睾酮、脑干神经递质5-HT的变化及TPH_2的基因表达。在第二个实验中,选择符合条件的大鼠随机分为6组:雌鼠空白组、雌鼠雌激素组、雌鼠孕激素组、雌鼠雄激素组、雄鼠空白组、雄鼠雌激素组。按不同组别分别于每日上午(雌鼠在动情期)给予高剂量的雌激素、孕激素、雄激素,连续3天,观察大鼠行为学、脑干神经递质5-HT及TPH_2基因表达的变化。结果:两个实验均表现出:高剂量雌激素可使大鼠反应性降低、迷宫总路程缩短,脑干5-羟色胺含量减少,Tph_2基因表达程度升高;实验大鼠表现出活动迟缓、反应淡漠、无抵抗、身体发软,说明在大鼠动情期给予外源性高剂量雌激素,可以造成实验大鼠出现抑郁状态。这种变化与利血平造模组变化趋同。经轻胶囊能改变动物的抑郁行为,增加大鼠迷宫总路程,降低E_2水平,增加脑干5-HT含量,降低Tph_2的基因表达。高剂量雄激素能升高大鼠行为总分,降低脑干5-HT灰度值,降低Tph_2基因表达程度;雌激素与雄激素对大鼠的影响有显著差异。结论:在大鼠动情期给予外源性高剂量雌二醇可造成大鼠抑郁状态,中药经轻胶囊可改善高剂量雌激素所造成抑郁状态大鼠的病理变化,纠正大鼠的抑郁行为。高剂量雌激素可降低脑干神经递质使大鼠出现抑郁,高剂量雄激素可增加脑干神经递质使大鼠出现兴奋。
Objective:The objective of this dissertation is to analyze the relationship between sex hormones and depression,to investigate intervention mechanisms of Jingqing capsules through the rat models of premenstrual depression state caused by exogenous high-dose-estrogen,to Explore inflection of sex hormone for rats' behaviors and 5-HT and gene expression of TPH_2 in brain stem,and analyze the relationship between menstrual depression and high-level-estrogen by injecting different sex hormones into different sex experimental rats.
Methods:We used animal model of three to four months old impregnated SD female and male rats, performed vaginal smear test for each female rats and set up the files of estrous cycle individually.We Choose rats according with demands,randomly divided the rats into eight different experimental groups,female rats contrast group,male rats contrast group,female rats Estradiol group,male rats Estradiol group,the reserpine model group,female rats Estradiol with Jingqing Capsule group,male rats Estradiol with Jingqing Capsule group,the reserpine model with Jingqing Capsule group.We injected Estradiol Benzoate into experimental rats on three consecutive mornings to introduce high-level-estrogen state,made the model during the estrous period.At the same time of modeling, given Jingqing Capsule for different group and observed changes of rats' behavioral,Estradiol, progestin,testosterone as well as changes of 5-HT and gene expression of TPH_2 in brain stem.Choose rats according with demands in the Second experiment,Randomly divided the rats into six different experimental groups:female rats contrast group,female rats Estradiol group,female rats progestagen group,female rats androgenic hormones group,.male rats contrast group,.male male Estradiol group. Given exogenous estrogen,progestagen and androgenic hormones on three consecutive mornings (during the estrous period of female).Observed changes of rats' behavioral changes as well as changes of 5-HT and gene expression of TPH_2 in brain stem.Results:high-dose-estrogen can decrease response degree to external stimulant,shorten rats' covered distance of y + labyrinth test,decrease the 5-HT content in brain stem,increase gene expression of TPH_2.Experiments rats are slowmoving, response is indifferent,body is soft and no resistance.These results show that exogenous high-dose-estrogen can cause depression in experimental rats when given during the late estrous period.These were also found in reserpine model group.Qingjing capsules can reduce depression in the animal,increase rats' covered distance of y + labyrinth test,decrease level of E_2.increase 5-HT content in the brain stem,decrease gene expression of TPH_2.High-dose-androgenic can increase rats' behavior total grade,decrease 5-HTgrey-number in the brain stem,decrease gene expression of TPH_2.These deferens between estradiol and androgenic hormones are obvious.Conclusion: Exogenous high-dose-estradiol during the estrous period can cause the premenstrual depression state in experimental rats,Jingqing capsules can reduce the pathological change,and hence the animal's depression can be relieved.Exogenous high-dose-estradiol can decrease experimental rats' quantities of neurotransmitter in the brain stem,hence experimental rats show depression.High-dose-androgenic hormones can increase rats' quantities of neurotransmitter in the brain stem,so experimental rats show excitation.
Objective:The objective of this dissertation is to analyze the relationship between sex hormones and depression,to investigate intervention mechanisms of Jingqing capsules through the rat models of premenstrual depression state caused by exogenous high-dose-estrogen,to Explore inflection of sex hormone for rats' behaviors and 5-HT and gene expression of TPH_2 in brain stem,and analyze the relationship between menstrual depression and high-level-estrogen by injecting different sex hormones into different sex experimental rats.
Methods:We used animal model of three to four months old impregnated SD female and male rats, performed vaginal smear test for each female rats and set up the files of estrous cycle individually.We Choose rats according with demands,randomly divided the rats into eight different experimental groups,female rats contrast group,male rats contrast group,female rats Estradiol group,male rats Estradiol group,the reserpine model group,female rats Estradiol with Jingqing Capsule group,male rats Estradiol with Jingqing Capsule group,the reserpine model with Jingqing Capsule group.We injected Estradiol Benzoate into experimental rats on three consecutive mornings to introduce high-level-estrogen state,made the model during the estrous period.At the same time of modeling, given Jingqing Capsule for different group and observed changes of rats' behavioral,Estradiol, progestin,testosterone as well as changes of 5-HT and gene expression of TPH_2 in brain stem.Choose rats according with demands in the Second experiment,Randomly divided the rats into six different experimental groups:female rats contrast group,female rats Estradiol group,female rats progestagen group,female rats androgenic hormones group,.male rats contrast group,.male male Estradiol group. Given exogenous estrogen,progestagen and androgenic hormones on three consecutive mornings (during the estrous period of female).Observed changes of rats' behavioral changes as well as changes of 5-HT and gene expression of TPH_2 in brain stem.Results:high-dose-estrogen can decrease response degree to external stimulant,shorten rats' covered distance of y + labyrinth test,decrease the 5-HT content in brain stem,increase gene expression of TPH_2.Experiments rats are slowmoving, response is indifferent,body is soft and no resistance.These results show that exogenous high-dose-estrogen can cause depression in experimental rats when given during the late estrous period.These were also found in reserpine model group.Qingjing capsules can reduce depression in the animal,increase rats' covered distance of y + labyrinth test,decrease level of E_2.increase 5-HT content in the brain stem,decrease gene expression of TPH_2.High-dose-androgenic can increase rats' behavior total grade,decrease 5-HTgrey-number in the brain stem,decrease gene expression of TPH_2.These deferens between estradiol and androgenic hormones are obvious.Conclusion: Exogenous high-dose-estradiol during the estrous period can cause the premenstrual depression state in experimental rats,Jingqing capsules can reduce the pathological change,and hence the animal's depression can be relieved.Exogenous high-dose-estradiol can decrease experimental rats' quantities of neurotransmitter in the brain stem,hence experimental rats show depression.High-dose-androgenic hormones can increase rats' quantities of neurotransmitter in the brain stem,so experimental rats show excitation.
引文
1.曹泽毅主编.中华妇产科学[M].北京:人民卫生出版社,2000:2222
2.王淑贞主编.实用妇产科学[M].北京:人民卫生出版社,1990:806
3.丰有吉主编.妇产科学[M].北京:人民卫生出版社,2002:322-324
4.靡若然主编.妇产科疾病诊断治疗学[M].中国医学科学技术出版社,2000:838
5.FrankRT.Hormonal cause of premenstrual tension[J].Arch Neurol psychiatry,1931,26:1053
6.乔明琦,张珍玉,徐旭杰,等.经前期综合症候分布规律的流行病学调查研究[J].中国中医基础医学杂志,1997,3(3):31-33
7.张金良,王超,赵茜,等.育龄女性月经前紧张综合征症状的初步调查[J].中国健康教育,2006,22(11):837-840
8.张德利,岳文浩,宋希俊,等.经前期综合征的心理症状研究[J].泰山医学院学报,2000,21(2):115-116
9.Lurics,BorensteinR.Premenstrualsyndrome[J].Obstel Gynecol Sury,1990,45(3):220
10.张学菊,宋博.经前期综合征与口服有机磷自杀关系的调查[J].护理学杂志,2003,8(1):61
11.赵勇.生物反馈治疗经前期综合征的研究进展[J].国外医学精神病学分册,2002,29(1):53-55
12.傅赛萍,王贤斌.经前安泰汤治疗经前紧张综合症228例[J].陕西中医,1997,18(6):241
13.王优快.经前平颗粒治疗经前期综合征60例临床体会[J].实用临床医药杂志,2003,7(1):82
14.苏巧荣,苏林雁,耿耀国.性激素及其受体基因多态性与青春期首发抑郁障碍[J].国际精神病学杂志,2006,33(1):59-63
15.姚芳传主编.情感性精神障碍[M].长沙:湖南科学技术出版社,1999:25-44
16.蒋先胜.雌激素与情感障碍(综述)[J].国外医学精神病学分册,2000,27(2):119-122
17.喻东山.性激素和抑郁症[J].国外医学精神病学分册,1999,26(2):111-112
18.徐文炜.雌激素与相关精神疾病[J].中华精神科杂志,2005,38(2):124-125
19.张郭莺,杨彦春.雌激素、单胺递质与抑郁症[J].国际精神病学杂志,2005,32(3):150-153
20.McEwen BS,Alves SE,Bulloch K,et al.Ovarian steroids and the brain:implication for cognition and aging.Neurology,1997,48(5):8-15
21.Thompson KS,Kulkami[J].Sergejew AA.Extrapyramidal symptoms and oestrogen.Acta pyschiatr scand,2000,101:130-134
22.Halbreich U,Rojansky N,Palter S,et al.Estrogen augments serotonergic activity in postmenopausal women[J].Biol Psychiatry,1995,37:434-441
23.McEwen BS,Alves SE,Bulloch K,et al.Ovarian steroids and the brain:implication for cognition and aging.Neurology,1997,48(5):8-15
24.Thompson KS,Kulkami J.Sergejew AA.Extrapyramidal symptoms and oestrogen.Acta pyschiatr scand,2000,101:130-134
25.刘春喜.发现第二种色胺酸羟化酶[J].生理科学进展,2003,34(3):254
26.张郭莺,杨彦春.雌激素、单胺递质与抑郁症[J].国际精神病学杂志,2005,32(3):150-153
27.相宇.经轻胶囊治疗高雌激素导致的类经前期综合征的实验研究.成都中医药大学博士学位,2004
28.朱鸿秋.雌二醇导致经行头痛的实验及经轻胶囊干预机制初探.成都中医药大学博士学位,2005
29.胡心伟.经轻胶囊对雌二醇诱发的经行抑郁状态干预机制的实验研究.成都中医药大学博士学位,2006
30.肖新春.经轻胶囊抗经前高雌抑郁的初步药效研究.成都中医药大学博士学位论文,2006
31.付金英.经轻胶囊干预动情后期高雌大鼠抑郁状态的实验研究.成都中医药大学博士学位论文,2006
32.刘屏.陈世铭.侯津详.高架十字迷宫和Vogel饮水冲突法用于吗啡依赖动物焦虑评价[J].中国药理学通报,1997,13(6):563-565
33.王欣,谢鸣.酸枣仁汤对高架十字迷宫大鼠行为学影响的量效关系评价[J].中国实验方剂学杂志,2004,10(1):35-37
34.乐杰主编.妇产科学[M].北京:人民卫生出版社,2005:236-238
35.龚绍麟.抑郁症[M].北京:人民卫生出版社,2003:127-131
36.Goodwin FK,Jamison KR et al.Manic Depressive illness[M].New York:Oxford Univerisity Press,1990:416-441
37.Schildkraut J.The catecholamine hypothesis of affective disorders:a review of supporting evidence[J].Am J Psychiatry.1965,122:509-512
38.McEwen BS,Alves SE,Bulloch K,et al.Ovarian steroids and the brain:implication for cognition and aging.Neurology,1997,48(5):8-15
39.Thompson KS,Kulkami J,Sergejew AA.Extrapyramidal symptoms and oestrogen.Acta pyschiatr scand,2000,101:130-134
40.李焕德,彭文兴.抑郁症药物治疗新进展[J].国外医学·精神病学分册,1997,24(3):129-133
41.蔡焯基.抑郁症基础与临床[M].北京:科学出版社,1997:1-76
42.郑伟,汪宝军,叶立民.灰度值、光学密度值与免疫组化片阳性表达强弱的关系[J].临床与实验病理学杂志,2003,19(5):566-567
43.许良中主编.实用肿瘤病理方法学[M].上海:上海医科大学出版社,1997:562-563
44.徐根兴.定量细胞学和细胞化学技术[M].吉林:吉林科学技术出版社,1993:14-25
45.宋景春,李志超,黄国明等.端粒酶逆转酶在五羟色胺诱导的大鼠肺动脉平滑肌细胞增殖过程中的作用[J].中华心血管病杂志,2006,34(9):837-842
46.McEwen BS,Alves SE,Bulloch K,et al.Ovarian steroids and the brain:implication for cognition and aging.Neurology,1997,48(5):8-15
47.Thompson KS,Kulkami J,Sergejew AA.Extrapyramidal symptoms and oestrogen.Acta pyschiatr scand,2000,101:130-134
48.Halbreich U,Rojansky N,Palter S,et al.Estrogen augments serotonergic activity in postmenopausal women[J].Biol Psychiatry,1995,37:434-441
49.Thompson KS,Kulkami J,Sergejew AA.Extrapyramidal symptoms and oestrogen.Acta pyschiatr scand,2000,101:130-134
50.张郭莺,杨彦春.雌激素、单胺递质与抑郁症[J].国际精神病学杂志,2005,32(3):150-153
51.苏巧荣,苏林雁,耿耀国.性激素及其受体基因多态性与青春期首发抑郁障碍[J].国际精神病学杂志,2006,33(1):59-63
52.Halbreich U.el al:Role of estrogen in the aetiology and treatment of moon disorders.CNS Drugs,2001,15(10):797-817
53.乐杰主编.妇产科学[M].北京:人民卫生出版社,1980:20-21
54.张郭莺,杨彦春.雌激素、单胺递质与抑郁症[J].国际精神病学杂志,2005,32(3):150-153
55.徐文炜.雌激素与相关精神疾病[J].中华精神科杂志,2005,38(2):124-125
56.Thompson KS,Kulkami J,Sergejew AA.Extrapyramidal symptoms and oestrogen.Acta pyschiatr scand,2000,101:130-134
57.Kimberly A,Yonkers MD:special issues related to the treatment of depression in women.J Clin Psychiatry,2003,84,(suppl 18):8-2
58.Rohr UD.The impact of testosterone imbalance on depression and women's health.Maturitas.2002,41(suppl 1):25-46
59.Ladisich w.Influence of progesterone on serotonin metabolism:a possible causal factor for mood changes.Psychoneuroen—docrinology,1977,290:257-266
60.Booth A,Johnson DR,Granger DA.Testosterone and men'sdepression:the role of social behavior.J Health Soc Behav 1999,40(2):130-40
61.李一云等.围绝经期综合症有关情绪障碍的研究进展[J].国际精神病学杂志,2005,32(4):229-232
62.Margolese 62Margolese H C.The male menopause and mood:testosterone decline and depression in the aging male-is there a link J Geriatr Psychiatry Neurol.2000,13(2):93-101
63.Hohlagsch wandtner M,Husslein P,Klier C,et al.Correlation between serum testosterone levels and peripartal mood states.Acta obstet Gymecol Scand.2001,80(4):326-30
64.ndrome to menopause.A hypotheses and review of supporting data.J Repord med.1996,41(9):633-9.
65.崔晓萍.论“女子以肝为先天”之内涵[J].陕西中医学院学报,1995,18(1):8-10
66.李晓秋,许晶.抑郁动物模型的研究进展[J].中华精神科杂志,2002,35(3):184-186
67.郭建友,李昌煜,葛卫红.抑郁症动物模型研究进展[J].中国临床康复,2004,8(10):1932-1933,
68.Porsolt RD,Lepichon M,Jalfre MI Depression.a new animal model sensitive to antidepressant treatmentl Nature,1977,266:7302-7321
69.Katz R,Hersch S1 Amitriptyline and scopolamine in an anima model of depressionl Neurosci Biobehav Rev,1981,5:2652-2711
70.金光亮,周东丰.慢性利血平抑郁模型大鼠脑内游离钙离子、钙调素和环核苷酸含量的变化[J].中华精神科杂志,1997,30(2):67-69
71.王海玲.复方降压片致抑郁症3例临床观察[J].中国医师杂志,2003,256
72.孙长友.复方降压片导致抑郁状态18例临床分析[J].临床精神医学杂志,1997,7(4):237
73.崔晓萍,张庆文.雌雄激素与中医阴阳及抑郁障碍的相关性研究[J].陕西中医,2007,28(7):862-864
74.孙广仁主编.全国高等中医药院校规划教材.中医基础理论[M].北京:中国中医药出版社,2002:33-43
75.程彤,阮金秀,袁淑兰,等.助阳类中草药对利血平化小鼠脑内儿茶酚胺递质和体征的影响[J].中草药,1992,23(12):657
76.沈政.生理心理学[M].北京:北京大学出版社,1994:641-642
77.徐淑云.药理实验方法学[M].北京:人民卫生出版社,1993:11
78.崔晓萍,张庆文.雌雄激素与中医阴阳及抑郁障碍的相关性研究[J].中国中医基础医学杂志,2008,2
79.Hohlagsch wandtner M,Husslein P,Klier C,et al.Correlation between serum testosterone levels and peripartal mood states.Acta obstet Gymecol Scand.2001,80(4):326-30
80.付金英.经轻胶囊干预动情后期高雌大鼠抑郁状态的实验研究.成都中医药大学 博士学位论文,2006,6
81.程彤,阮金秀,袁淑兰,等.助阳类中草药对利血平化小鼠脑内儿茶酚胺递质和体征的影响[J].中草药,1992,23(12):657
82.钱菁.“助阳解郁汤”治疗经前期紧张综合征32例[J].江苏中医,1999,20(12):28
83.孙广仁主编.全国高等中医药院校规划教材.中医基础理论[M].北京:中国中医药出版社,2002:225-229
1.曹泽毅主编.中华妇产科学[M].北京:人民卫生出版社,2000:2222
2.王淑贞主编.实用妇产科学[M].北京:人民卫生出版社,1990:806
3.丰有吉主编.妇产科学[M].人民卫生出版社,2002:322-324
4.靡若然主编.妇产科疾病诊断治疗学[M].中国医学科学技术出版社,2000:838
5.FrankRT.Hormonal cause of premenstrual tension[J].Arch Neural psychiatry,1931,26:1053
6.world health organization international classification of disease[R].Genev a:WHO,1996.2
7.Frackiewicz AJ and ShiovitzTM.Evaluation and management of premenstrual syndrome and premenstrual dysphonic disorder[J].J of American pharmaceutical association,2000,41(3):437-447
8.Lurics,BorensteinR.Premenstrual syndrome[J].Obstel Gynecol Sury,1990,45(3):220
9.乔明琦,张珍玉,徐旭杰,等.经前期综合症候分布规律的流行病学调查研究[J].中国中医基础医学杂志,1997,3(3):31-33
10.张金良,王超,赵茜等.育龄女性月经前紧张综合征症状的初步调查[J].中国健康教育,2006,22(11):837-840
11.张德利,岳文浩,宋希俊,等.经前期综合征的心理症状研究[J].泰山医学院学 报,2000,21(2):115-116
12.张学菊,宋博.经前期综合征与口服有机磷自杀关系的调查[J].护理学杂志,2003,18(1):61
13.乔明琦,张珍玉.经前期紧张综合征证候分布规律的流行病学调查研究[J].中国中医基础医学杂志,1997,3(3):31-32
14.张金良,王超,赵茜,等.育龄女性月经前紧张综合征症状的初步调查[J].中国健康教育,2006,22(11):837-840
15.张德利,岳文浩,宋希俊,等.经前期综合征的心理症状研究[J].泰山医学院学报,2000,21(2):115-116
16.孙晓玲,许丽绵.经前期综合征的中医学研究进展[J].中医药信息,2006,23(3):9-11
17.张艳辉.经前期综合征的中医药治疗研究进展[J].吉林中医药,2003,23(11):55-56
18.李书文.逍遥散加减治疗经前期综合征46例[J].河南中医,2006,26(5):66
19.钱菁.“助阳解郁汤”治疗经前期紧张综合征32例[J].江苏中医,1999,20(12):28
20.MarilynI,etal:ClinicalObstetricsandGynecology,1997,40(3):564-576
21.张郭莺,杨彦春.雌激素、单胺递质与抑郁症[J].国际精神病学杂志,2005,32(3):150-153
22.McEwen BS,Alves SE,Bulloch K,et al.Ovarian steroids and the brain:implication for cognition and aging.Neurology,1997,48(5):8-15
23.Thompson KS,Kulkami J,Sergejew AA.Extrapyramidal symptoms and oestrogen.Acta pyschiatr scand,2000,101:130-134
24.张翠莲,张伟琪,朱珠.盐酸氟西汀—治疗经前期紧张综合征[J].中国药学杂志,2001,36(9):642-643
25.李元敏.选择性5-羟色胺重摄取抑制剂对严重的经前期综合征的疗效[J].国外医学药学分册,2001,2(2)122-123
26.王海军,乔明琦,张惠云.经前期综合征(PMS)肝气逆证猕猴造模及用药血清中性激素检测与分析[J].中药药理与临床,2006,22(5)58,
27.魏霞,张惠云,乔明琦.经前平颗粒对经前期综合征肝气逆证患者血清E_((2))、P峰值的影响[J].山东中医药大学学报,2006,30(3):198-200
28.傅赛萍,王贤斌.经前安泰汤治疗经前紧张综合症228例[J].陕西中医,1997,18(6):241
29.王优快.经前平颗粒治疗经前期综合征60例临床体会[J].实用临床医药杂志,2003,7(1):82
30.孙广仁主编.中医基础理论[M].北京:中国中医药出版社,2002:225-229
1.乐杰主编.妇产科学[M].北京:人民卫生出版社,2005:236-238.
2.张学菊,宋博.经前期综合征与口服有机磷自杀关系的调查[J].护理学杂志,2003,18(1):61
3.乔明琦,张珍玉.经前期紧张综合征证候分布规律的流行病学调查研究[J].中国中医基础医学杂志,1997,3(3):31-32
4.Kimberly A,Yonkers MD:special issues related to the treatment of depression in women.J Clin Psychiatry,2003,64,(suppl 18):8-2
5.乐杰主编.妇产科学[M].人民卫生出版社,1980:20-21
6.徐文炜.雌激素与相关精神疾病[J].中华精神科杂志,2005,38(2):124-125.
7.McEwen BS,Alves SE,Bulloch K,et al.Ovarian steroids and the brain:implication for cognition and aging.Neurology,1997,48(57):8-15
8.Thompson KS,Kulkami J,Sergejew AA.Extrapyramidal symptoms and oestrogen.Acta pyschiatr scand,2000,101:130-134
9.张郭莺,杨彦春.雌激素、单胺递质与抑郁症[J].国际精神病学杂志,2005,32(3):150-153
10.苏巧荣,苏林雁,耿耀国.性激素及其受体基因多态性与青春期首发抑郁障碍[J].国际精神病学杂志.2006,33(1):59-63
11.Halbreich U.el al:Role of estrogen in the aetiology and treatment of moon disorders.CNS Drugs,2001,15(10):797-817
1.苏巧荣,苏林雁,耿耀国.性激素及其受体基因多态性与青春期首发抑郁障碍.国际精神病学杂志,2006,33(1):59-63
2.姚芳传主编.情感性精神障碍[M].湖南:湖南科学技术出版社,1999:25-44
3.蒋先胜.雌激素与情感障碍(综述[J]).国外医学精神病学分册,2000,27(2):119-122
4.徐文炜.雌激素与相关精神疾病[J].中华精神科杂志,2005,38(2):124-125
5.张郭莺,杨彦春:雌激素、单胺递质与抑郁症.国际精神病学杂志.2005,32(3):150-153
6.Booth A,Johnson DR,Granger DA.Testosterone and men'sdepression:the role of social behavior.J Health Soc Behav 1999,40(2):130
7.Margolese H C.The male menopause and mood:testosterone decline and depression in the aging male-is there a link J Geriatr Psychiatry Neurol.2000,13(2):93-101
8.Hohlagsch wandtner M,Husslein P,Klier C,et al.Correlation between serum testosterone levels and peripartal mood states.Acta obstet Gymecol Scand.2001,80(4):326
9.Arpels JC.The female brain hypoestrogenic continuum from the premenstrual syndrome to menopause.A hypotheses and review of supporting data.J Repord med.1996,41(9):633
10.McEwen BS,Alves SE,Bulloch K,et al.Ovarian steroids and the brain:implication for cognition and aging.Neurology,1997,48(5):8-15
11.Thompson KS,Kulkami J,Sergejew AA.Extrapyramidal symptoms and oestrogen.Acta pyschiatr scand,2000,101:130-134
12.张郭莺,杨彦春.雌激素、单胺递质与抑郁症[J].国际精神病学杂志,2005:32(3):150-153
13.Halbreich U.el al:Role of estrogen in the aetiology and treatment of moon disorders.CNS Drugs,2001,15(10):797-817
14.乐杰主编.妇产科学[M].北京:人民卫生出版社,1980:20-21
15.Kimberly A,Yonkers MD:special issues related to the treatment of depression in women.J Clin Psychiatry,2003,64,(suppl 18):8-2
16.Rohr UD:The impact of testosterone imbalance on depression and women's health.Maturitas.2002,41(suppl 1):25-46
17.Ladisich w:Influence of progesterone on serotonin metabolism:a possible causal factor for mood changes.Psychoneuroen-docrinology,1977,290:257-266
18.李一云等.围绝经期综合症有关情绪障碍的研究进展[J].国际精神病学杂志,2005,32(4):229-232
19.孙广仁主编。全国高等中医药院校规划教材.中医基础理论[M].北京:中国中医药出版社,2002:33-43
20.付金英.经轻胶囊干预动情后期高雌大鼠抑郁状态的实验研究.成都中医药大学博士学位论文,2006,6
2.王淑贞主编.实用妇产科学[M].北京:人民卫生出版社,1990:806
3.丰有吉主编.妇产科学[M].北京:人民卫生出版社,2002:322-324
4.靡若然主编.妇产科疾病诊断治疗学[M].中国医学科学技术出版社,2000:838
5.FrankRT.Hormonal cause of premenstrual tension[J].Arch Neurol psychiatry,1931,26:1053
6.乔明琦,张珍玉,徐旭杰,等.经前期综合症候分布规律的流行病学调查研究[J].中国中医基础医学杂志,1997,3(3):31-33
7.张金良,王超,赵茜,等.育龄女性月经前紧张综合征症状的初步调查[J].中国健康教育,2006,22(11):837-840
8.张德利,岳文浩,宋希俊,等.经前期综合征的心理症状研究[J].泰山医学院学报,2000,21(2):115-116
9.Lurics,BorensteinR.Premenstrualsyndrome[J].Obstel Gynecol Sury,1990,45(3):220
10.张学菊,宋博.经前期综合征与口服有机磷自杀关系的调查[J].护理学杂志,2003,8(1):61
11.赵勇.生物反馈治疗经前期综合征的研究进展[J].国外医学精神病学分册,2002,29(1):53-55
12.傅赛萍,王贤斌.经前安泰汤治疗经前紧张综合症228例[J].陕西中医,1997,18(6):241
13.王优快.经前平颗粒治疗经前期综合征60例临床体会[J].实用临床医药杂志,2003,7(1):82
14.苏巧荣,苏林雁,耿耀国.性激素及其受体基因多态性与青春期首发抑郁障碍[J].国际精神病学杂志,2006,33(1):59-63
15.姚芳传主编.情感性精神障碍[M].长沙:湖南科学技术出版社,1999:25-44
16.蒋先胜.雌激素与情感障碍(综述)[J].国外医学精神病学分册,2000,27(2):119-122
17.喻东山.性激素和抑郁症[J].国外医学精神病学分册,1999,26(2):111-112
18.徐文炜.雌激素与相关精神疾病[J].中华精神科杂志,2005,38(2):124-125
19.张郭莺,杨彦春.雌激素、单胺递质与抑郁症[J].国际精神病学杂志,2005,32(3):150-153
20.McEwen BS,Alves SE,Bulloch K,et al.Ovarian steroids and the brain:implication for cognition and aging.Neurology,1997,48(5):8-15
21.Thompson KS,Kulkami[J].Sergejew AA.Extrapyramidal symptoms and oestrogen.Acta pyschiatr scand,2000,101:130-134
22.Halbreich U,Rojansky N,Palter S,et al.Estrogen augments serotonergic activity in postmenopausal women[J].Biol Psychiatry,1995,37:434-441
23.McEwen BS,Alves SE,Bulloch K,et al.Ovarian steroids and the brain:implication for cognition and aging.Neurology,1997,48(5):8-15
24.Thompson KS,Kulkami J.Sergejew AA.Extrapyramidal symptoms and oestrogen.Acta pyschiatr scand,2000,101:130-134
25.刘春喜.发现第二种色胺酸羟化酶[J].生理科学进展,2003,34(3):254
26.张郭莺,杨彦春.雌激素、单胺递质与抑郁症[J].国际精神病学杂志,2005,32(3):150-153
27.相宇.经轻胶囊治疗高雌激素导致的类经前期综合征的实验研究.成都中医药大学博士学位,2004
28.朱鸿秋.雌二醇导致经行头痛的实验及经轻胶囊干预机制初探.成都中医药大学博士学位,2005
29.胡心伟.经轻胶囊对雌二醇诱发的经行抑郁状态干预机制的实验研究.成都中医药大学博士学位,2006
30.肖新春.经轻胶囊抗经前高雌抑郁的初步药效研究.成都中医药大学博士学位论文,2006
31.付金英.经轻胶囊干预动情后期高雌大鼠抑郁状态的实验研究.成都中医药大学博士学位论文,2006
32.刘屏.陈世铭.侯津详.高架十字迷宫和Vogel饮水冲突法用于吗啡依赖动物焦虑评价[J].中国药理学通报,1997,13(6):563-565
33.王欣,谢鸣.酸枣仁汤对高架十字迷宫大鼠行为学影响的量效关系评价[J].中国实验方剂学杂志,2004,10(1):35-37
34.乐杰主编.妇产科学[M].北京:人民卫生出版社,2005:236-238
35.龚绍麟.抑郁症[M].北京:人民卫生出版社,2003:127-131
36.Goodwin FK,Jamison KR et al.Manic Depressive illness[M].New York:Oxford Univerisity Press,1990:416-441
37.Schildkraut J.The catecholamine hypothesis of affective disorders:a review of supporting evidence[J].Am J Psychiatry.1965,122:509-512
38.McEwen BS,Alves SE,Bulloch K,et al.Ovarian steroids and the brain:implication for cognition and aging.Neurology,1997,48(5):8-15
39.Thompson KS,Kulkami J,Sergejew AA.Extrapyramidal symptoms and oestrogen.Acta pyschiatr scand,2000,101:130-134
40.李焕德,彭文兴.抑郁症药物治疗新进展[J].国外医学·精神病学分册,1997,24(3):129-133
41.蔡焯基.抑郁症基础与临床[M].北京:科学出版社,1997:1-76
42.郑伟,汪宝军,叶立民.灰度值、光学密度值与免疫组化片阳性表达强弱的关系[J].临床与实验病理学杂志,2003,19(5):566-567
43.许良中主编.实用肿瘤病理方法学[M].上海:上海医科大学出版社,1997:562-563
44.徐根兴.定量细胞学和细胞化学技术[M].吉林:吉林科学技术出版社,1993:14-25
45.宋景春,李志超,黄国明等.端粒酶逆转酶在五羟色胺诱导的大鼠肺动脉平滑肌细胞增殖过程中的作用[J].中华心血管病杂志,2006,34(9):837-842
46.McEwen BS,Alves SE,Bulloch K,et al.Ovarian steroids and the brain:implication for cognition and aging.Neurology,1997,48(5):8-15
47.Thompson KS,Kulkami J,Sergejew AA.Extrapyramidal symptoms and oestrogen.Acta pyschiatr scand,2000,101:130-134
48.Halbreich U,Rojansky N,Palter S,et al.Estrogen augments serotonergic activity in postmenopausal women[J].Biol Psychiatry,1995,37:434-441
49.Thompson KS,Kulkami J,Sergejew AA.Extrapyramidal symptoms and oestrogen.Acta pyschiatr scand,2000,101:130-134
50.张郭莺,杨彦春.雌激素、单胺递质与抑郁症[J].国际精神病学杂志,2005,32(3):150-153
51.苏巧荣,苏林雁,耿耀国.性激素及其受体基因多态性与青春期首发抑郁障碍[J].国际精神病学杂志,2006,33(1):59-63
52.Halbreich U.el al:Role of estrogen in the aetiology and treatment of moon disorders.CNS Drugs,2001,15(10):797-817
53.乐杰主编.妇产科学[M].北京:人民卫生出版社,1980:20-21
54.张郭莺,杨彦春.雌激素、单胺递质与抑郁症[J].国际精神病学杂志,2005,32(3):150-153
55.徐文炜.雌激素与相关精神疾病[J].中华精神科杂志,2005,38(2):124-125
56.Thompson KS,Kulkami J,Sergejew AA.Extrapyramidal symptoms and oestrogen.Acta pyschiatr scand,2000,101:130-134
57.Kimberly A,Yonkers MD:special issues related to the treatment of depression in women.J Clin Psychiatry,2003,84,(suppl 18):8-2
58.Rohr UD.The impact of testosterone imbalance on depression and women's health.Maturitas.2002,41(suppl 1):25-46
59.Ladisich w.Influence of progesterone on serotonin metabolism:a possible causal factor for mood changes.Psychoneuroen—docrinology,1977,290:257-266
60.Booth A,Johnson DR,Granger DA.Testosterone and men'sdepression:the role of social behavior.J Health Soc Behav 1999,40(2):130-40
61.李一云等.围绝经期综合症有关情绪障碍的研究进展[J].国际精神病学杂志,2005,32(4):229-232
62.Margolese 62Margolese H C.The male menopause and mood:testosterone decline and depression in the aging male-is there a link J Geriatr Psychiatry Neurol.2000,13(2):93-101
63.Hohlagsch wandtner M,Husslein P,Klier C,et al.Correlation between serum testosterone levels and peripartal mood states.Acta obstet Gymecol Scand.2001,80(4):326-30
64.ndrome to menopause.A hypotheses and review of supporting data.J Repord med.1996,41(9):633-9.
65.崔晓萍.论“女子以肝为先天”之内涵[J].陕西中医学院学报,1995,18(1):8-10
66.李晓秋,许晶.抑郁动物模型的研究进展[J].中华精神科杂志,2002,35(3):184-186
67.郭建友,李昌煜,葛卫红.抑郁症动物模型研究进展[J].中国临床康复,2004,8(10):1932-1933,
68.Porsolt RD,Lepichon M,Jalfre MI Depression.a new animal model sensitive to antidepressant treatmentl Nature,1977,266:7302-7321
69.Katz R,Hersch S1 Amitriptyline and scopolamine in an anima model of depressionl Neurosci Biobehav Rev,1981,5:2652-2711
70.金光亮,周东丰.慢性利血平抑郁模型大鼠脑内游离钙离子、钙调素和环核苷酸含量的变化[J].中华精神科杂志,1997,30(2):67-69
71.王海玲.复方降压片致抑郁症3例临床观察[J].中国医师杂志,2003,256
72.孙长友.复方降压片导致抑郁状态18例临床分析[J].临床精神医学杂志,1997,7(4):237
73.崔晓萍,张庆文.雌雄激素与中医阴阳及抑郁障碍的相关性研究[J].陕西中医,2007,28(7):862-864
74.孙广仁主编.全国高等中医药院校规划教材.中医基础理论[M].北京:中国中医药出版社,2002:33-43
75.程彤,阮金秀,袁淑兰,等.助阳类中草药对利血平化小鼠脑内儿茶酚胺递质和体征的影响[J].中草药,1992,23(12):657
76.沈政.生理心理学[M].北京:北京大学出版社,1994:641-642
77.徐淑云.药理实验方法学[M].北京:人民卫生出版社,1993:11
78.崔晓萍,张庆文.雌雄激素与中医阴阳及抑郁障碍的相关性研究[J].中国中医基础医学杂志,2008,2
79.Hohlagsch wandtner M,Husslein P,Klier C,et al.Correlation between serum testosterone levels and peripartal mood states.Acta obstet Gymecol Scand.2001,80(4):326-30
80.付金英.经轻胶囊干预动情后期高雌大鼠抑郁状态的实验研究.成都中医药大学 博士学位论文,2006,6
81.程彤,阮金秀,袁淑兰,等.助阳类中草药对利血平化小鼠脑内儿茶酚胺递质和体征的影响[J].中草药,1992,23(12):657
82.钱菁.“助阳解郁汤”治疗经前期紧张综合征32例[J].江苏中医,1999,20(12):28
83.孙广仁主编.全国高等中医药院校规划教材.中医基础理论[M].北京:中国中医药出版社,2002:225-229
1.曹泽毅主编.中华妇产科学[M].北京:人民卫生出版社,2000:2222
2.王淑贞主编.实用妇产科学[M].北京:人民卫生出版社,1990:806
3.丰有吉主编.妇产科学[M].人民卫生出版社,2002:322-324
4.靡若然主编.妇产科疾病诊断治疗学[M].中国医学科学技术出版社,2000:838
5.FrankRT.Hormonal cause of premenstrual tension[J].Arch Neural psychiatry,1931,26:1053
6.world health organization international classification of disease[R].Genev a:WHO,1996.2
7.Frackiewicz AJ and ShiovitzTM.Evaluation and management of premenstrual syndrome and premenstrual dysphonic disorder[J].J of American pharmaceutical association,2000,41(3):437-447
8.Lurics,BorensteinR.Premenstrual syndrome[J].Obstel Gynecol Sury,1990,45(3):220
9.乔明琦,张珍玉,徐旭杰,等.经前期综合症候分布规律的流行病学调查研究[J].中国中医基础医学杂志,1997,3(3):31-33
10.张金良,王超,赵茜等.育龄女性月经前紧张综合征症状的初步调查[J].中国健康教育,2006,22(11):837-840
11.张德利,岳文浩,宋希俊,等.经前期综合征的心理症状研究[J].泰山医学院学 报,2000,21(2):115-116
12.张学菊,宋博.经前期综合征与口服有机磷自杀关系的调查[J].护理学杂志,2003,18(1):61
13.乔明琦,张珍玉.经前期紧张综合征证候分布规律的流行病学调查研究[J].中国中医基础医学杂志,1997,3(3):31-32
14.张金良,王超,赵茜,等.育龄女性月经前紧张综合征症状的初步调查[J].中国健康教育,2006,22(11):837-840
15.张德利,岳文浩,宋希俊,等.经前期综合征的心理症状研究[J].泰山医学院学报,2000,21(2):115-116
16.孙晓玲,许丽绵.经前期综合征的中医学研究进展[J].中医药信息,2006,23(3):9-11
17.张艳辉.经前期综合征的中医药治疗研究进展[J].吉林中医药,2003,23(11):55-56
18.李书文.逍遥散加减治疗经前期综合征46例[J].河南中医,2006,26(5):66
19.钱菁.“助阳解郁汤”治疗经前期紧张综合征32例[J].江苏中医,1999,20(12):28
20.MarilynI,etal:ClinicalObstetricsandGynecology,1997,40(3):564-576
21.张郭莺,杨彦春.雌激素、单胺递质与抑郁症[J].国际精神病学杂志,2005,32(3):150-153
22.McEwen BS,Alves SE,Bulloch K,et al.Ovarian steroids and the brain:implication for cognition and aging.Neurology,1997,48(5):8-15
23.Thompson KS,Kulkami J,Sergejew AA.Extrapyramidal symptoms and oestrogen.Acta pyschiatr scand,2000,101:130-134
24.张翠莲,张伟琪,朱珠.盐酸氟西汀—治疗经前期紧张综合征[J].中国药学杂志,2001,36(9):642-643
25.李元敏.选择性5-羟色胺重摄取抑制剂对严重的经前期综合征的疗效[J].国外医学药学分册,2001,2(2)122-123
26.王海军,乔明琦,张惠云.经前期综合征(PMS)肝气逆证猕猴造模及用药血清中性激素检测与分析[J].中药药理与临床,2006,22(5)58,
27.魏霞,张惠云,乔明琦.经前平颗粒对经前期综合征肝气逆证患者血清E_((2))、P峰值的影响[J].山东中医药大学学报,2006,30(3):198-200
28.傅赛萍,王贤斌.经前安泰汤治疗经前紧张综合症228例[J].陕西中医,1997,18(6):241
29.王优快.经前平颗粒治疗经前期综合征60例临床体会[J].实用临床医药杂志,2003,7(1):82
30.孙广仁主编.中医基础理论[M].北京:中国中医药出版社,2002:225-229
1.乐杰主编.妇产科学[M].北京:人民卫生出版社,2005:236-238.
2.张学菊,宋博.经前期综合征与口服有机磷自杀关系的调查[J].护理学杂志,2003,18(1):61
3.乔明琦,张珍玉.经前期紧张综合征证候分布规律的流行病学调查研究[J].中国中医基础医学杂志,1997,3(3):31-32
4.Kimberly A,Yonkers MD:special issues related to the treatment of depression in women.J Clin Psychiatry,2003,64,(suppl 18):8-2
5.乐杰主编.妇产科学[M].人民卫生出版社,1980:20-21
6.徐文炜.雌激素与相关精神疾病[J].中华精神科杂志,2005,38(2):124-125.
7.McEwen BS,Alves SE,Bulloch K,et al.Ovarian steroids and the brain:implication for cognition and aging.Neurology,1997,48(57):8-15
8.Thompson KS,Kulkami J,Sergejew AA.Extrapyramidal symptoms and oestrogen.Acta pyschiatr scand,2000,101:130-134
9.张郭莺,杨彦春.雌激素、单胺递质与抑郁症[J].国际精神病学杂志,2005,32(3):150-153
10.苏巧荣,苏林雁,耿耀国.性激素及其受体基因多态性与青春期首发抑郁障碍[J].国际精神病学杂志.2006,33(1):59-63
11.Halbreich U.el al:Role of estrogen in the aetiology and treatment of moon disorders.CNS Drugs,2001,15(10):797-817
1.苏巧荣,苏林雁,耿耀国.性激素及其受体基因多态性与青春期首发抑郁障碍.国际精神病学杂志,2006,33(1):59-63
2.姚芳传主编.情感性精神障碍[M].湖南:湖南科学技术出版社,1999:25-44
3.蒋先胜.雌激素与情感障碍(综述[J]).国外医学精神病学分册,2000,27(2):119-122
4.徐文炜.雌激素与相关精神疾病[J].中华精神科杂志,2005,38(2):124-125
5.张郭莺,杨彦春:雌激素、单胺递质与抑郁症.国际精神病学杂志.2005,32(3):150-153
6.Booth A,Johnson DR,Granger DA.Testosterone and men'sdepression:the role of social behavior.J Health Soc Behav 1999,40(2):130
7.Margolese H C.The male menopause and mood:testosterone decline and depression in the aging male-is there a link J Geriatr Psychiatry Neurol.2000,13(2):93-101
8.Hohlagsch wandtner M,Husslein P,Klier C,et al.Correlation between serum testosterone levels and peripartal mood states.Acta obstet Gymecol Scand.2001,80(4):326
9.Arpels JC.The female brain hypoestrogenic continuum from the premenstrual syndrome to menopause.A hypotheses and review of supporting data.J Repord med.1996,41(9):633
10.McEwen BS,Alves SE,Bulloch K,et al.Ovarian steroids and the brain:implication for cognition and aging.Neurology,1997,48(5):8-15
11.Thompson KS,Kulkami J,Sergejew AA.Extrapyramidal symptoms and oestrogen.Acta pyschiatr scand,2000,101:130-134
12.张郭莺,杨彦春.雌激素、单胺递质与抑郁症[J].国际精神病学杂志,2005:32(3):150-153
13.Halbreich U.el al:Role of estrogen in the aetiology and treatment of moon disorders.CNS Drugs,2001,15(10):797-817
14.乐杰主编.妇产科学[M].北京:人民卫生出版社,1980:20-21
15.Kimberly A,Yonkers MD:special issues related to the treatment of depression in women.J Clin Psychiatry,2003,64,(suppl 18):8-2
16.Rohr UD:The impact of testosterone imbalance on depression and women's health.Maturitas.2002,41(suppl 1):25-46
17.Ladisich w:Influence of progesterone on serotonin metabolism:a possible causal factor for mood changes.Psychoneuroen-docrinology,1977,290:257-266
18.李一云等.围绝经期综合症有关情绪障碍的研究进展[J].国际精神病学杂志,2005,32(4):229-232
19.孙广仁主编。全国高等中医药院校规划教材.中医基础理论[M].北京:中国中医药出版社,2002:33-43
20.付金英.经轻胶囊干预动情后期高雌大鼠抑郁状态的实验研究.成都中医药大学博士学位论文,2006,6
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |