小麦种子蛋白及赤霉病抗性相关基因的分子鉴定
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摘要
依据功能不同可将种子蛋白划分为贮藏功能的谷醇溶蛋白和维持正常代谢功能的非醇溶性蛋白两类。高分子量谷蛋白亚基(HMW-GS)属于种子醇溶性蛋白,优质HMW-GS可明显改善小麦加工品质。小麦近缘属种中广泛存在的HMW-GS等位变异基因可能成为小麦品质改良的重要基因资源。麦类作物种子中还富含属于种子非醇溶性蛋白中清蛋白的α-淀粉酶抑制因子(AAI),能有效抑制内源或外源α-淀粉酶活性,控制种子穗发芽、抑制昆虫繁殖。赤霉病是小麦主要病害之一,易感染开花期的小穗和发育早期的籽粒。赤霉菌会扰乱种子贮藏蛋白的合成或者消耗种子中的蛋白、糖来供自己繁殖,从而破坏发育中的籽粒。本文对小麦种子蛋白HMW-GS、AAI进行了基因克隆、与进化分析,并对抗赤霉菌材料的基因表达谱进行了分子鉴定,主要结果如下:
1.从二倍体长穗偃麦草中分离并鉴定了4个新型HMW-GS编码基因,分别编码x-型亚基(Ee2.1、Ee1.9)与y-型亚基(Ee1.8、Ee1.5)。其中,Ee2.1和Ee1.5各存在1个与普通小麦HMW-GS相比特殊的Cys,推测这两个亚基在小麦品质育种中具有潜在利用价值。对小麦族不同物种HMW-GS的N-端序列进行比对分析发现,x-型和y-型HMW-GS均具有很高的同源性,但y-型HMW-GS变异程度比x-型亚基小。依据是否存在15bp或24bp的碱基插入将x-型HMW-GS及其编码基因划分为3种类型。系统进化分析表明,HMW-GS编码基因在漫长的基因复制过程中相对独立,且一直保持着其生物学功能。来自普通小麦A、B、D基因组的HMW-GS存在明显差异,具有染色体组特异性。
2.从普通小麦及近缘物种中克隆获得与昆虫抗性有关的635个二聚α-淀粉酶抑制因子编码基因(WDAI),377个单聚α-淀粉酶抑制因子(WMAI)和20个四聚α-淀粉酶抑制因子(WTAI)编码基因序列。对α-淀粉酶抑制因子序列与功能分析,探讨了该家族成员间的理化、功能(如等电点、迁移率、对蛋白酶的抑制范围和活性)差异的分子基础。建立了一种利用序列中SNP位点开发出特异标记引物将功能基因定位于普通六倍体小麦具体基因组的方法,由此将来自普通小麦的WDAI基因定位在3BS和3DS上。虽然从A基因组一粒系小麦中得到了WDAI、WMAI编码基因序列,但分子证据揭示普通小麦A基因组不含有WDAI编码基因,而是否含有WMAI编码基因则需要进一步研究。依据序列特征,推测小麦及其近缘物种中WDAI源于共同的祖先基因,而小麦及山羊草的WMAI也可能由一共同的祖先基因通过复制与突变进化而来,但是WMAI相似性明显比WDAI高,变异程度更低。
3.依据序列分析发现二倍体一粒系小麦由于碱基插入导致无α-淀粉酶抑制因子活性的分子基础。一粒系小麦中WDAI编码基因差异与基因组来源有关,与地理来源关系不明显。A~m基因组的WDAI基因与A~u基因组的基因相比进化速率更慢、变异程度更低。依据普通小麦B基因组和拟斯卑尔托山羊草组S(S、S~l、S~s、S~(sh)和S~b)基因组159个WDAI基因序列中SNP位点差异,共发现59种呈“星”形分布的单倍型,并可归为5个主要的单倍型组。普通小麦和野生二粒小麦B基因组上的WDAI基因与来自拟斯卑尔脱山羊草S基因组上的WDAI基因最相似,但是推测多个拟斯卑尔托山羊草组物种可能通过种间杂交时的基因渐渗参与了多倍体小麦B基因组WDAI基因位点的形成。小麦族11个属20个基因组的WDAI基因的分子系统进化分析表明,WDAI编码基因差异与基因组系统进化有关,且在基因组分化以前该位点就已经存在。这些结果为深入了解WDAI基因位点上基因重组、整合事件及发生过程提供了重要信息,有助于加深对WDAI功能的了解。
4.对以色列野生二粒小麦α-淀粉酶抑制因子基因的遗传结构、分化程度及其与生态因子间的关系进行了系统研究。WMAI序列所包含遗传信息较低;WTAI序列同样非常一致。无论是在居群内还是居群间,野生二粒小麦WDAI基因位点都存在变异,居群间遗传距离与地理来源关系密切。野生二粒小麦WDAI基因序列ω值(Ka/Ks)大于1,表明该基因位点为正选择位点,存在明显的选择压力导致WDAI基因序列产生多样性及遗传和功能差异。WDAI编码基因中SNP标记所揭示的16个居群遗传多样性指数(P)、Nei's基因多样性(He)和Shannon指数(I)分别为0.887、0.404和0.589。SNP服从于自然选择,生态因子单独或联合作用导致WDAI基因序列中SNP位点的多样性,特别是几个水分因子对SNP位点有极显著影响,推测水分作为主要选择压力通过影响昆虫数量间接影响α-淀粉酶抑制因子遗传结构变异、分化程度。
5.获得与过敏性哮喘相关的小麦种子潜在变应原编码基因。利用生物信息学手段对小麦种子蛋白质变应原与公认主要变应原α-淀粉酶抑制因子进行了比较,并预测编码蛋白质高级结构。由于过敏哮喘与由IgE介导的超敏反应有关,推测这些蛋白质具有能与IgE结合相似的结构域。
6.对一套中国春-长穗偃麦草附加系、代换系、端体异附加系材料的赤霉病抗性进行了评价,发现7E附加系(CS-7E)和7ES端体异附加系(CS-YES)抗性最强,推测7E染色体上存在潜在的赤霉病抗性基因或能诱导/抑制普通小麦CS上抗性/易感基因表达的调控基因。利用基因芯片对CS、CS-7E、CS-7ES在赤霉菌诱导0-96小时后的表达谱进行了分析,初步筛选出70个赤霉病菌诱导差异表达的抗性相关基因;生物信息学比对和功能注解发现,候选基因所编码蛋白质或是属于某些特定的生理生化代谢途径关键酶、或是已知功能真菌诱导抗性应答蛋白、或与生物或非生物胁迫应答有关。利用实时定量PCR对16个候选基因的表达水平进行了验证,与芯片数据相比基因表达水平变化的方向(上调或下调)一致,但Q-PCR所检测到的差异要大于芯片所显示的表达差异。
A major class of wheat storage proteins is the high-molecular-weight(HMW) glutenins,which play a crucial role in determining the quality of wheat due to its contribution to the dough visco-elastic properties.The visco-elastic properties of bread wheat flour are associated with the formation of disulphide bridge-linked aggregates, through several cysteines(Cys)of HMW gluten peptides in the terminal,non-repetitive domains of the polypeptide,and the central domain composed of repeats of short peptide motifs.It is well established that there were multiple forms of proteins active on exogenous or endogenousα-amylases in wheat kernel,and proteinaceous dimericα-amylase inhibitors with inhibitory activity were againstα-amylase of various origins.It was known that the bulk of wheat albumins consisted of a few amylase isoinhibitor families very likely phylogenetically related and coded by a small number of parental genes.For weevil control,α-amylase inhibitors could be used through plant genetic engineering.However,many insects have severalα-amylases that differ in specificity,and successful utilization of a food source is dependent on the presence of aα-amylase for which there is no specific inhibitor.The structure and function of dimericα-amylase inhibitor genes with different cSNPs by direct sequencing,which is the most direct way to identify SNP polymorphisms, from cultivated wheat varieties and its diploid putative progenitor,were investigated. Fusarium head blight(FHB)is a major disease of cereals associated with at least seventeen Fusarium species,although there is no strong evidence for race-specific resistance.The disease can cause significant yield losses and reduce grain quality due to the production of mycotoxins.Breeding for resistance has taken a high priority worldwide,as genetic resistance,although partial,is considered to offer the most promising tool for the control of associated mycotoxin contamination in harvested grain.The mechanisms of resistance to FHB are complex and as yet,not fully understood and consequently breeding has been hampered by the difficulty of incorporating resistance into adapted high-yielding cultivars. The precise reasons for the lack of progress in incorporating exotic resistances into well-adapted commercial cultivars are not known.However,this lack of progress has led to a greater interest in understanding FHB resistance mechanisms.Our results about storage proteins were described as follows:
1.Four high-molecular-weight glutenin subunit(HMW-GS)genes from Elytrigia elongata(Host)Nevski were characterized by determining the coding sequences of two x-type subunit genes Ee2.1 and Ee1.9,and two y-type subunit gene Ee1.8 and Ee1.5 with 2082,1938 1788 and 1488bp,respectively.The numbers of amino acids in the central repeat domains of these E genome glutenin subunits were considerably fewer than the other known HMW-GSs with substitutions,insertions and/or deletions involving a single or more amino acid residues.In spite of the high similarity at both 5' and 3' regions,Ee1.5 had only 1492 bp with several major deletions in its middle region.Thus,Ee1.5 is one of the smallest known HMW-GS genes.The N-terminal domain of Ee1.5 has 105 amino acid residues,while the other known y-type HMWGSs all have 104 amino acid residues. Comparison of Ee1.5 and 1Dy10 showed that Ee1.5 had a Cys residue at the middle of repetitive domain,while the Cys at the last of repetitive domain,which was common in other y-type HMW-GSs,was lost in Ee1.5.The altered Cys at the repetitive domain may likely have impact on the inter- or intra-molecular disulphide bonds.Moreover,an extra cysteine(Cys)was found in the repeated domain of x-type subunit Ee2.1.The difference in the number and position of Cys residues might be associated with the good dough quality. The extra Cys in the good-quality subunit Dx5 was at the beginning of the repetitive domain,while the extra Cys in Ee2.1 was at the central repetitive domain.
To investigate the molecular evolution of the high-molecular-weight glutenin subunits (HMW-GSs)in Triticeae,multiple sequence alignment was carried out on the N-terminal sequences of 77 HMW-GSs.The sequence of N-terminal domain showed high homology in both x- and y-type subunits.Sequence alignment of the N-terminal domain of y-type subunits suggested that they shared similar primary structure with each other,not only in the length variation of the N-terminal domain but also they have less variation in amino acids in this domain than those of x-type subunits.Analysis of the x-type HMW-GSs genes revealed that there were three conservative HMW-GSs gene groups with distinct fragments at the beginning of the N-terminal domains of ORFs,defined by the presence or absence of 24 bp fragment,and the presence of 15 bp fragment,respectively.The frequency of mutations in the x-type and y-type HMW-GSs genes was 1.89 and 1.25 out of 10 bases, respectively.Sequence analysis revealed that the y-type HMW-GSs did not contain enough variation for evolutionary analyses.It is obvious that the HMW-GSs encoded by genes of A,B and D genomes in common wheat formed three clusters.The x-type HMW-GSs from Aegilops bicornis,Aegilops comosa,Aegilops cylindrica,Aegilops umbellulata and Aegilops uniaristata were more closely related to the subunits from the D genome rather than those from the B genome.
2.α-Amylases inhibitors are attractive candidates for the control of seed weevils as these insects are highly dependent on starch as an energy source.For weevil control,α-amylases inhibitors and their genes could be used to genetically engineer weevil resistant seeds.
Seventeen new genes encoding 24 kDa family dimericα-amylases inhibitors had been characterized from cultivated wheat and its diploid putative progenitors.And the differentα-amylases inhibitors in this family,which were determined by coding regions single nucleotide polymorphisms(cSNPs)of their genes,were investigated.The amino acid sequences of 24 kDaα-amylases inhibitors shared very high coherence(91.2%).It indicated that the dimericα-amylases inhibitors in the 24 kDa family were derived from common ancestral genes by phylogenetic analysis.Eightα-amylases inhibitor genes were characterized from one hexaploid wheat variety,and clustered into four subgroups, indicating that the 24 kDa dimericα-amylases inhibitors in cultivated wheat were encoded by multi-gene.Forty-five cSNPs,including 35 transitions and 10 transversions,were found, and resulted in a total of ten amino acid changes.The cSNPs at the first site of a codon cause much more nonsynonymous(92.9%)than synonymous mutations,while nonsynonymous and synonymous mutations were almost equal when the cSNPs were at the third site.It was observed that there was Ile105 instead of Val105 at the active region Vai104-Val105-Asp106-Ala107 of theα-amylases inhibitor by cSNPs in some inhibitors from Aegilops speltoides,diploid and hexaploid wheats.
Thirty genes encoding dimericα-amylases inhibitors were isolated from Triticum aestivum L.'Chinese Spring' and characterized by nucleotide and amino acid sequence analysis.Eleven representativeα-amylases inhibitor genes were identified and the deduced amino acid sequences of these genes were of high coherence(95.1%).These inhibitors and others obtained from the wheat EST database were clustered into three groups,the genes from 'Chinese Spring' were present in each group.Specific primer sets were designed for each group,based on the SNPs of these genes,and the chromosome locations of each group of inhibitor genes investigated by amplification of the 'Chinese Spring' ditelosomic lines.There were two and one groups of inhibitor genes on chromosomes 3BS and 3DS, respectively,whereas no group of inhibitor genes was found on chromosome 3AS.Thus, the primer set for each group of inhibitor genes was genome allele-specific.The two known inhibitors,0.53 and 0.19,were located on chromosomes 3BS and 3DS,respectively. The validity of the three genome allele-specific primer sets was confirmed by amplifications in 15 accessions of Triticum urartu,Triticum monococcum,Aegilops tauschii and Triticum dicoccoides.These results gave further support at the molecular level, that the 24 kDa dimericα-amylases inhibitors in cultivated wheat are encoded by a multigene family.
Seventy-three gene sequences encoding monomericα-amylase inhibitors were characterized from cultivated wheat "Chinese Spring",group 6 nullisomic-tetrasomic lines of "Chinese Spring" and diploid putative progenitors of common wheat.The monomericα-amylase inhibitors from the different sources shared very high homology(99.54%).The differentα-amylase inhibitors,which were determined by the 24 single nucleotide polymorphisms(SN-Ps)of their gene sequences,were investigated.A total of 15 haplotypes were defined by sequence alignment,among which 9 haplotypes were found with only one single sequence sample.Haplotype H02 was found to be the main haplotype occurring in 83 WMAI sequence samples,followed by haplotype H11.The median-joining network for the 15 haplotypes of monomericα-amylase inhibitor gene sequences from hexaploid wheats was star like,and at least two subclusters emerged.Furthermore evidence of homologous recombination was found between the haplotypes.The relationship between nucleotide substitutions and the amino acid changes in WMAI of hexaploid wheats was summarized.It was clear that only 5 polymorphic sites in the nucleotide sequence of WMAI resulted in amino acid variations,and that should be the reason for different structure and function of inhibitors.However,little evidence could be found that there were WMAI genes in the A genome of hexaploid wheat,whereas it could conclude from our results that the A genome diploid wheat had WMAI genes.The overall information on the monomericα-amylase inhibitors from wheat and Aegilops strongly support the view that these inhibitors have evolved from a common ancestral gene through duplication and mutation.
3.Eighty dimericα-amylases inhibitor genes were characterized from 68 accessions of the einkorn wheats Triticum urartu,T.boeoticum,and T.monococcum.The mature protein coding sequences of WDAI genes were analyzed.Nucleotide sequence variations in these regions resulted from base substitution and/or indel mutations.Most of the WDAI gene sequences from T.boeoticum and all sequences from T.monococcum had one nucleotide insertion in the coding region,such that theseα-amylases inhibitor sequences could not encode the correct mature proteins.We identified 21 distinct haplotypes from the diploid wheat WDAI gene sequences.A main haplotype was found in 15 gene samples from the Au genome and 35 gene samples from the Am genome.The T.monococcum and T. boeoticum accessions shared the same main haplotype,with 25 samples from T. monococcum and 10 from T.boeoticum.The WDAI gene sequences from the Au and Am genomes could be obviously clustered into two clades,but the sequences from the Am genome of T.boeoticum and T.monococcum could not be clearly distinguished.The phylogenetic analysis revealed that the WDAI gene sequences from the Am genome had accumulated fewer variations and evolved at a slower rate than the sequences from the Au genome.Although some accessions from only one or two areas had unique mutations at the same position,the diversity of WDAI gene sequences in diploid wheat showed little relationship to the origin of the accessions.
One hundred and fifty-nine sequences encoding dimericα-amylases inhibitors were characterized from Triticum and Aegilops.These sequences had 375 nucleotides in length, among which there were 255 conserved sites,50 singleton variable sites(the nucleotide polymorphism only observed in a single accession)and 70 polymorphic sites(the polymorphisms found in two or more accessions).The frequency of SNPs in the B(a.k.a.S) genomes codingα-amylases inhibitor genes was 1.7 out of 10 bases.A total of 59 haplotypes were defined,among which 4 main haplotypes occurring in more than 10 genes and 36 haplotypes with single gene were found,indicating that the dimericα-amylases inhibitors might derive from a very limited number of ancestral genes.The phylogenetic median-joining network of the 59 haplotypes was highly star like with 5 haplotype groups, and at least 2 sub clusters emerged.Evolutionary distances of the 159 genes were calculated,and subjected to the construction of neighbour-joining trees that showed that theα-amylases inhibitor genes were divided into 5 groups,and each group had at least 2 subgroups.The neighbour-joining tree of the species indicated that the genes from common wheat and Triticum dicoccoides were closely related to those from Aegilops speltoides,and it was further supported by the median-joining networks analysis of the 59 haplotypes.These results revealed the important information on genome shaping events and processes occurring at dimericα-amylases inhibitor genes loci and contributed to our understanding of functional aspects of the dimericα-amylases inhibitor genes,as well as phylogenetic relationships between species.
4.In this study,we aimed to reveal the structure and diversity of dimericα-amylase inhibitor genes in wild emmer wheat from Israel and to elucidate the relationship between the emmer wheat genes and ecological factors using single nucleotide polymorphism(SNP) markers.Another objective of this study was to find out whether there were any correlations between SNPs in functional protein-coding genes and the environment.The influence of ecological factors on the genetic structure of dimericα-amylase inhibitor genes was evaluated by specific SNP markers.A total of 244 dimericα-amylase inhibitor genes were obtained from 13 accessions in 10 populations.Seventy-five polymorphic positions and 74 haplotypes were defined by sequence analysis.Sixteen out of the 75 SNP markers were designed to detect SNP variations in wild emmer wheat accessions from different populations in Israel.The proportion of polymorphic loci P(5%),the expected heterozygosity He,and Shannon's information index in the 16 populations were 0.887, 0.404,and 0.589,respectively.The populations of wild emmer wheat showed great diversity in gene loci both between and within populations.Based on the SNP marker data, the genetic distance of pair-wise comparisons of the 16 populations displayed a sharp genetic differentiation over long geographic distances.The values of P,He,and Shannon's reformation index were negatively correlated with three climatic moisture factors,whereas the same values were positively correlated by Spearman rank correlation coefficients' analysis with some of the other ecological factors.The populations of wild emmer wheat showed a wide range of diversity in dimericα-amylase inhibitors,both between and within populations.We suggested that SNP markers are useful for the estimation of genetic diversity of functional genes in wild emmer wheat.These results show significant correlations between SNPs in theα-amylase inhibitor genes and ecological factors affecting diversity.Ecological factors,singly or in combination,explained a significant proportion of the variations in the SNPs,and the SNPs could be classified into several categories as ecogeographical predictors.It was suggested that the SNPs in theα-amylase inhibitor genes have been subjected to natural selection,and ecological factors had an important evolutionary influence on gene differentiation at specific loci.
5.Genes encoding five allergens(WMAI,WDAI,WTAI,Fructose bisphosphate aldolase and Glycerinaldehyde-3-phosphate dehydrogenases)associated with baker's asthma were obtained from wheat seed.Compared with other known allergens,the protein structures were calculated by bioinformatics analysis.They had different biology function in plant.Only the three amylase inhibitors had similar amino acid sequences and 3D structure,while others were different.For the baker's asthma was IgE-mediated hypersensitivity,it could be concluded that these allergens had similar domain combined with IgE.
6.Fusarium head blight(FHB)is a major disease of cereals associated with at least seventeen Fusarium species,although there is no strong evidence for race-specific resistance.Here we report the evaluation of many of wheat-Thinopyrum addition lines, substitution lines and ditelosomic addition lines in cv.Chinese Spring(CS)for resistance to the spread of Fusarium graminearum in wheat heads.The lines CS-7E and CS-7ES showed the highest resistance levels.To gain some insights about the molecular mechanism(s)of resistance in those lines,large scale RNA profiling was performed on CS-7E,CS-7ES and CS.
We analyzed gene expression changes in spike of Fusarium infected and uninfected at 3 time points during disease development.829 genes(probe sets)were found to be mote than 5 fold differentially expressed in at least one time point from at least one wheat lines. It was clear that more up-regulated expression genes were found than down-regulated genes after Fusarium infection.Obvious numerical features that could be seen in the Figureure were that the numbers of up- and down-regulated genes were same in CS,but the down-regulated genes in CS-7E(S)were 2.5 fold more than up-regulated genes.To identify groups of genes with similar expression patterns,a hierarchical clustering algorithm to the 70 genes with most different expression level that induced at any of the time points in response to fungal infection were carried out.The 70 genes were clustered into 5 groups by their expression level,and belonged to many different function categories. It was found some candidate genes were belong to specific biochemical pathways or known to have functional associations and responding to fungal infection.Many changes in potential phenylpropanoid pathway genes were observed including genes associated with the salicylic acid(SA)mediated response.Genes involved in the biosynthesis of Jasmonic acid(JA)were prominently found to responsive to fungal stress treatment in CS, CS-7E and CS-7ES in this study too.
1.从二倍体长穗偃麦草中分离并鉴定了4个新型HMW-GS编码基因,分别编码x-型亚基(Ee2.1、Ee1.9)与y-型亚基(Ee1.8、Ee1.5)。其中,Ee2.1和Ee1.5各存在1个与普通小麦HMW-GS相比特殊的Cys,推测这两个亚基在小麦品质育种中具有潜在利用价值。对小麦族不同物种HMW-GS的N-端序列进行比对分析发现,x-型和y-型HMW-GS均具有很高的同源性,但y-型HMW-GS变异程度比x-型亚基小。依据是否存在15bp或24bp的碱基插入将x-型HMW-GS及其编码基因划分为3种类型。系统进化分析表明,HMW-GS编码基因在漫长的基因复制过程中相对独立,且一直保持着其生物学功能。来自普通小麦A、B、D基因组的HMW-GS存在明显差异,具有染色体组特异性。
2.从普通小麦及近缘物种中克隆获得与昆虫抗性有关的635个二聚α-淀粉酶抑制因子编码基因(WDAI),377个单聚α-淀粉酶抑制因子(WMAI)和20个四聚α-淀粉酶抑制因子(WTAI)编码基因序列。对α-淀粉酶抑制因子序列与功能分析,探讨了该家族成员间的理化、功能(如等电点、迁移率、对蛋白酶的抑制范围和活性)差异的分子基础。建立了一种利用序列中SNP位点开发出特异标记引物将功能基因定位于普通六倍体小麦具体基因组的方法,由此将来自普通小麦的WDAI基因定位在3BS和3DS上。虽然从A基因组一粒系小麦中得到了WDAI、WMAI编码基因序列,但分子证据揭示普通小麦A基因组不含有WDAI编码基因,而是否含有WMAI编码基因则需要进一步研究。依据序列特征,推测小麦及其近缘物种中WDAI源于共同的祖先基因,而小麦及山羊草的WMAI也可能由一共同的祖先基因通过复制与突变进化而来,但是WMAI相似性明显比WDAI高,变异程度更低。
3.依据序列分析发现二倍体一粒系小麦由于碱基插入导致无α-淀粉酶抑制因子活性的分子基础。一粒系小麦中WDAI编码基因差异与基因组来源有关,与地理来源关系不明显。A~m基因组的WDAI基因与A~u基因组的基因相比进化速率更慢、变异程度更低。依据普通小麦B基因组和拟斯卑尔托山羊草组S(S、S~l、S~s、S~(sh)和S~b)基因组159个WDAI基因序列中SNP位点差异,共发现59种呈“星”形分布的单倍型,并可归为5个主要的单倍型组。普通小麦和野生二粒小麦B基因组上的WDAI基因与来自拟斯卑尔脱山羊草S基因组上的WDAI基因最相似,但是推测多个拟斯卑尔托山羊草组物种可能通过种间杂交时的基因渐渗参与了多倍体小麦B基因组WDAI基因位点的形成。小麦族11个属20个基因组的WDAI基因的分子系统进化分析表明,WDAI编码基因差异与基因组系统进化有关,且在基因组分化以前该位点就已经存在。这些结果为深入了解WDAI基因位点上基因重组、整合事件及发生过程提供了重要信息,有助于加深对WDAI功能的了解。
4.对以色列野生二粒小麦α-淀粉酶抑制因子基因的遗传结构、分化程度及其与生态因子间的关系进行了系统研究。WMAI序列所包含遗传信息较低;WTAI序列同样非常一致。无论是在居群内还是居群间,野生二粒小麦WDAI基因位点都存在变异,居群间遗传距离与地理来源关系密切。野生二粒小麦WDAI基因序列ω值(Ka/Ks)大于1,表明该基因位点为正选择位点,存在明显的选择压力导致WDAI基因序列产生多样性及遗传和功能差异。WDAI编码基因中SNP标记所揭示的16个居群遗传多样性指数(P)、Nei's基因多样性(He)和Shannon指数(I)分别为0.887、0.404和0.589。SNP服从于自然选择,生态因子单独或联合作用导致WDAI基因序列中SNP位点的多样性,特别是几个水分因子对SNP位点有极显著影响,推测水分作为主要选择压力通过影响昆虫数量间接影响α-淀粉酶抑制因子遗传结构变异、分化程度。
5.获得与过敏性哮喘相关的小麦种子潜在变应原编码基因。利用生物信息学手段对小麦种子蛋白质变应原与公认主要变应原α-淀粉酶抑制因子进行了比较,并预测编码蛋白质高级结构。由于过敏哮喘与由IgE介导的超敏反应有关,推测这些蛋白质具有能与IgE结合相似的结构域。
6.对一套中国春-长穗偃麦草附加系、代换系、端体异附加系材料的赤霉病抗性进行了评价,发现7E附加系(CS-7E)和7ES端体异附加系(CS-YES)抗性最强,推测7E染色体上存在潜在的赤霉病抗性基因或能诱导/抑制普通小麦CS上抗性/易感基因表达的调控基因。利用基因芯片对CS、CS-7E、CS-7ES在赤霉菌诱导0-96小时后的表达谱进行了分析,初步筛选出70个赤霉病菌诱导差异表达的抗性相关基因;生物信息学比对和功能注解发现,候选基因所编码蛋白质或是属于某些特定的生理生化代谢途径关键酶、或是已知功能真菌诱导抗性应答蛋白、或与生物或非生物胁迫应答有关。利用实时定量PCR对16个候选基因的表达水平进行了验证,与芯片数据相比基因表达水平变化的方向(上调或下调)一致,但Q-PCR所检测到的差异要大于芯片所显示的表达差异。
A major class of wheat storage proteins is the high-molecular-weight(HMW) glutenins,which play a crucial role in determining the quality of wheat due to its contribution to the dough visco-elastic properties.The visco-elastic properties of bread wheat flour are associated with the formation of disulphide bridge-linked aggregates, through several cysteines(Cys)of HMW gluten peptides in the terminal,non-repetitive domains of the polypeptide,and the central domain composed of repeats of short peptide motifs.It is well established that there were multiple forms of proteins active on exogenous or endogenousα-amylases in wheat kernel,and proteinaceous dimericα-amylase inhibitors with inhibitory activity were againstα-amylase of various origins.It was known that the bulk of wheat albumins consisted of a few amylase isoinhibitor families very likely phylogenetically related and coded by a small number of parental genes.For weevil control,α-amylase inhibitors could be used through plant genetic engineering.However,many insects have severalα-amylases that differ in specificity,and successful utilization of a food source is dependent on the presence of aα-amylase for which there is no specific inhibitor.The structure and function of dimericα-amylase inhibitor genes with different cSNPs by direct sequencing,which is the most direct way to identify SNP polymorphisms, from cultivated wheat varieties and its diploid putative progenitor,were investigated. Fusarium head blight(FHB)is a major disease of cereals associated with at least seventeen Fusarium species,although there is no strong evidence for race-specific resistance.The disease can cause significant yield losses and reduce grain quality due to the production of mycotoxins.Breeding for resistance has taken a high priority worldwide,as genetic resistance,although partial,is considered to offer the most promising tool for the control of associated mycotoxin contamination in harvested grain.The mechanisms of resistance to FHB are complex and as yet,not fully understood and consequently breeding has been hampered by the difficulty of incorporating resistance into adapted high-yielding cultivars. The precise reasons for the lack of progress in incorporating exotic resistances into well-adapted commercial cultivars are not known.However,this lack of progress has led to a greater interest in understanding FHB resistance mechanisms.Our results about storage proteins were described as follows:
1.Four high-molecular-weight glutenin subunit(HMW-GS)genes from Elytrigia elongata(Host)Nevski were characterized by determining the coding sequences of two x-type subunit genes Ee2.1 and Ee1.9,and two y-type subunit gene Ee1.8 and Ee1.5 with 2082,1938 1788 and 1488bp,respectively.The numbers of amino acids in the central repeat domains of these E genome glutenin subunits were considerably fewer than the other known HMW-GSs with substitutions,insertions and/or deletions involving a single or more amino acid residues.In spite of the high similarity at both 5' and 3' regions,Ee1.5 had only 1492 bp with several major deletions in its middle region.Thus,Ee1.5 is one of the smallest known HMW-GS genes.The N-terminal domain of Ee1.5 has 105 amino acid residues,while the other known y-type HMWGSs all have 104 amino acid residues. Comparison of Ee1.5 and 1Dy10 showed that Ee1.5 had a Cys residue at the middle of repetitive domain,while the Cys at the last of repetitive domain,which was common in other y-type HMW-GSs,was lost in Ee1.5.The altered Cys at the repetitive domain may likely have impact on the inter- or intra-molecular disulphide bonds.Moreover,an extra cysteine(Cys)was found in the repeated domain of x-type subunit Ee2.1.The difference in the number and position of Cys residues might be associated with the good dough quality. The extra Cys in the good-quality subunit Dx5 was at the beginning of the repetitive domain,while the extra Cys in Ee2.1 was at the central repetitive domain.
To investigate the molecular evolution of the high-molecular-weight glutenin subunits (HMW-GSs)in Triticeae,multiple sequence alignment was carried out on the N-terminal sequences of 77 HMW-GSs.The sequence of N-terminal domain showed high homology in both x- and y-type subunits.Sequence alignment of the N-terminal domain of y-type subunits suggested that they shared similar primary structure with each other,not only in the length variation of the N-terminal domain but also they have less variation in amino acids in this domain than those of x-type subunits.Analysis of the x-type HMW-GSs genes revealed that there were three conservative HMW-GSs gene groups with distinct fragments at the beginning of the N-terminal domains of ORFs,defined by the presence or absence of 24 bp fragment,and the presence of 15 bp fragment,respectively.The frequency of mutations in the x-type and y-type HMW-GSs genes was 1.89 and 1.25 out of 10 bases, respectively.Sequence analysis revealed that the y-type HMW-GSs did not contain enough variation for evolutionary analyses.It is obvious that the HMW-GSs encoded by genes of A,B and D genomes in common wheat formed three clusters.The x-type HMW-GSs from Aegilops bicornis,Aegilops comosa,Aegilops cylindrica,Aegilops umbellulata and Aegilops uniaristata were more closely related to the subunits from the D genome rather than those from the B genome.
2.α-Amylases inhibitors are attractive candidates for the control of seed weevils as these insects are highly dependent on starch as an energy source.For weevil control,α-amylases inhibitors and their genes could be used to genetically engineer weevil resistant seeds.
Seventeen new genes encoding 24 kDa family dimericα-amylases inhibitors had been characterized from cultivated wheat and its diploid putative progenitors.And the differentα-amylases inhibitors in this family,which were determined by coding regions single nucleotide polymorphisms(cSNPs)of their genes,were investigated.The amino acid sequences of 24 kDaα-amylases inhibitors shared very high coherence(91.2%).It indicated that the dimericα-amylases inhibitors in the 24 kDa family were derived from common ancestral genes by phylogenetic analysis.Eightα-amylases inhibitor genes were characterized from one hexaploid wheat variety,and clustered into four subgroups, indicating that the 24 kDa dimericα-amylases inhibitors in cultivated wheat were encoded by multi-gene.Forty-five cSNPs,including 35 transitions and 10 transversions,were found, and resulted in a total of ten amino acid changes.The cSNPs at the first site of a codon cause much more nonsynonymous(92.9%)than synonymous mutations,while nonsynonymous and synonymous mutations were almost equal when the cSNPs were at the third site.It was observed that there was Ile105 instead of Val105 at the active region Vai104-Val105-Asp106-Ala107 of theα-amylases inhibitor by cSNPs in some inhibitors from Aegilops speltoides,diploid and hexaploid wheats.
Thirty genes encoding dimericα-amylases inhibitors were isolated from Triticum aestivum L.'Chinese Spring' and characterized by nucleotide and amino acid sequence analysis.Eleven representativeα-amylases inhibitor genes were identified and the deduced amino acid sequences of these genes were of high coherence(95.1%).These inhibitors and others obtained from the wheat EST database were clustered into three groups,the genes from 'Chinese Spring' were present in each group.Specific primer sets were designed for each group,based on the SNPs of these genes,and the chromosome locations of each group of inhibitor genes investigated by amplification of the 'Chinese Spring' ditelosomic lines.There were two and one groups of inhibitor genes on chromosomes 3BS and 3DS, respectively,whereas no group of inhibitor genes was found on chromosome 3AS.Thus, the primer set for each group of inhibitor genes was genome allele-specific.The two known inhibitors,0.53 and 0.19,were located on chromosomes 3BS and 3DS,respectively. The validity of the three genome allele-specific primer sets was confirmed by amplifications in 15 accessions of Triticum urartu,Triticum monococcum,Aegilops tauschii and Triticum dicoccoides.These results gave further support at the molecular level, that the 24 kDa dimericα-amylases inhibitors in cultivated wheat are encoded by a multigene family.
Seventy-three gene sequences encoding monomericα-amylase inhibitors were characterized from cultivated wheat "Chinese Spring",group 6 nullisomic-tetrasomic lines of "Chinese Spring" and diploid putative progenitors of common wheat.The monomericα-amylase inhibitors from the different sources shared very high homology(99.54%).The differentα-amylase inhibitors,which were determined by the 24 single nucleotide polymorphisms(SN-Ps)of their gene sequences,were investigated.A total of 15 haplotypes were defined by sequence alignment,among which 9 haplotypes were found with only one single sequence sample.Haplotype H02 was found to be the main haplotype occurring in 83 WMAI sequence samples,followed by haplotype H11.The median-joining network for the 15 haplotypes of monomericα-amylase inhibitor gene sequences from hexaploid wheats was star like,and at least two subclusters emerged.Furthermore evidence of homologous recombination was found between the haplotypes.The relationship between nucleotide substitutions and the amino acid changes in WMAI of hexaploid wheats was summarized.It was clear that only 5 polymorphic sites in the nucleotide sequence of WMAI resulted in amino acid variations,and that should be the reason for different structure and function of inhibitors.However,little evidence could be found that there were WMAI genes in the A genome of hexaploid wheat,whereas it could conclude from our results that the A genome diploid wheat had WMAI genes.The overall information on the monomericα-amylase inhibitors from wheat and Aegilops strongly support the view that these inhibitors have evolved from a common ancestral gene through duplication and mutation.
3.Eighty dimericα-amylases inhibitor genes were characterized from 68 accessions of the einkorn wheats Triticum urartu,T.boeoticum,and T.monococcum.The mature protein coding sequences of WDAI genes were analyzed.Nucleotide sequence variations in these regions resulted from base substitution and/or indel mutations.Most of the WDAI gene sequences from T.boeoticum and all sequences from T.monococcum had one nucleotide insertion in the coding region,such that theseα-amylases inhibitor sequences could not encode the correct mature proteins.We identified 21 distinct haplotypes from the diploid wheat WDAI gene sequences.A main haplotype was found in 15 gene samples from the Au genome and 35 gene samples from the Am genome.The T.monococcum and T. boeoticum accessions shared the same main haplotype,with 25 samples from T. monococcum and 10 from T.boeoticum.The WDAI gene sequences from the Au and Am genomes could be obviously clustered into two clades,but the sequences from the Am genome of T.boeoticum and T.monococcum could not be clearly distinguished.The phylogenetic analysis revealed that the WDAI gene sequences from the Am genome had accumulated fewer variations and evolved at a slower rate than the sequences from the Au genome.Although some accessions from only one or two areas had unique mutations at the same position,the diversity of WDAI gene sequences in diploid wheat showed little relationship to the origin of the accessions.
One hundred and fifty-nine sequences encoding dimericα-amylases inhibitors were characterized from Triticum and Aegilops.These sequences had 375 nucleotides in length, among which there were 255 conserved sites,50 singleton variable sites(the nucleotide polymorphism only observed in a single accession)and 70 polymorphic sites(the polymorphisms found in two or more accessions).The frequency of SNPs in the B(a.k.a.S) genomes codingα-amylases inhibitor genes was 1.7 out of 10 bases.A total of 59 haplotypes were defined,among which 4 main haplotypes occurring in more than 10 genes and 36 haplotypes with single gene were found,indicating that the dimericα-amylases inhibitors might derive from a very limited number of ancestral genes.The phylogenetic median-joining network of the 59 haplotypes was highly star like with 5 haplotype groups, and at least 2 sub clusters emerged.Evolutionary distances of the 159 genes were calculated,and subjected to the construction of neighbour-joining trees that showed that theα-amylases inhibitor genes were divided into 5 groups,and each group had at least 2 subgroups.The neighbour-joining tree of the species indicated that the genes from common wheat and Triticum dicoccoides were closely related to those from Aegilops speltoides,and it was further supported by the median-joining networks analysis of the 59 haplotypes.These results revealed the important information on genome shaping events and processes occurring at dimericα-amylases inhibitor genes loci and contributed to our understanding of functional aspects of the dimericα-amylases inhibitor genes,as well as phylogenetic relationships between species.
4.In this study,we aimed to reveal the structure and diversity of dimericα-amylase inhibitor genes in wild emmer wheat from Israel and to elucidate the relationship between the emmer wheat genes and ecological factors using single nucleotide polymorphism(SNP) markers.Another objective of this study was to find out whether there were any correlations between SNPs in functional protein-coding genes and the environment.The influence of ecological factors on the genetic structure of dimericα-amylase inhibitor genes was evaluated by specific SNP markers.A total of 244 dimericα-amylase inhibitor genes were obtained from 13 accessions in 10 populations.Seventy-five polymorphic positions and 74 haplotypes were defined by sequence analysis.Sixteen out of the 75 SNP markers were designed to detect SNP variations in wild emmer wheat accessions from different populations in Israel.The proportion of polymorphic loci P(5%),the expected heterozygosity He,and Shannon's information index in the 16 populations were 0.887, 0.404,and 0.589,respectively.The populations of wild emmer wheat showed great diversity in gene loci both between and within populations.Based on the SNP marker data, the genetic distance of pair-wise comparisons of the 16 populations displayed a sharp genetic differentiation over long geographic distances.The values of P,He,and Shannon's reformation index were negatively correlated with three climatic moisture factors,whereas the same values were positively correlated by Spearman rank correlation coefficients' analysis with some of the other ecological factors.The populations of wild emmer wheat showed a wide range of diversity in dimericα-amylase inhibitors,both between and within populations.We suggested that SNP markers are useful for the estimation of genetic diversity of functional genes in wild emmer wheat.These results show significant correlations between SNPs in theα-amylase inhibitor genes and ecological factors affecting diversity.Ecological factors,singly or in combination,explained a significant proportion of the variations in the SNPs,and the SNPs could be classified into several categories as ecogeographical predictors.It was suggested that the SNPs in theα-amylase inhibitor genes have been subjected to natural selection,and ecological factors had an important evolutionary influence on gene differentiation at specific loci.
5.Genes encoding five allergens(WMAI,WDAI,WTAI,Fructose bisphosphate aldolase and Glycerinaldehyde-3-phosphate dehydrogenases)associated with baker's asthma were obtained from wheat seed.Compared with other known allergens,the protein structures were calculated by bioinformatics analysis.They had different biology function in plant.Only the three amylase inhibitors had similar amino acid sequences and 3D structure,while others were different.For the baker's asthma was IgE-mediated hypersensitivity,it could be concluded that these allergens had similar domain combined with IgE.
6.Fusarium head blight(FHB)is a major disease of cereals associated with at least seventeen Fusarium species,although there is no strong evidence for race-specific resistance.Here we report the evaluation of many of wheat-Thinopyrum addition lines, substitution lines and ditelosomic addition lines in cv.Chinese Spring(CS)for resistance to the spread of Fusarium graminearum in wheat heads.The lines CS-7E and CS-7ES showed the highest resistance levels.To gain some insights about the molecular mechanism(s)of resistance in those lines,large scale RNA profiling was performed on CS-7E,CS-7ES and CS.
We analyzed gene expression changes in spike of Fusarium infected and uninfected at 3 time points during disease development.829 genes(probe sets)were found to be mote than 5 fold differentially expressed in at least one time point from at least one wheat lines. It was clear that more up-regulated expression genes were found than down-regulated genes after Fusarium infection.Obvious numerical features that could be seen in the Figureure were that the numbers of up- and down-regulated genes were same in CS,but the down-regulated genes in CS-7E(S)were 2.5 fold more than up-regulated genes.To identify groups of genes with similar expression patterns,a hierarchical clustering algorithm to the 70 genes with most different expression level that induced at any of the time points in response to fungal infection were carried out.The 70 genes were clustered into 5 groups by their expression level,and belonged to many different function categories. It was found some candidate genes were belong to specific biochemical pathways or known to have functional associations and responding to fungal infection.Many changes in potential phenylpropanoid pathway genes were observed including genes associated with the salicylic acid(SA)mediated response.Genes involved in the biosynthesis of Jasmonic acid(JA)were prominently found to responsive to fungal stress treatment in CS, CS-7E and CS-7ES in this study too.
引文
1. Aarts MGM, Keijzer CJ, Stiekema WJ, Pereira A. Molecular Characterization of the CER7 Gene of Arabidopsis Involved in Epicuticular Wax Biosynthesis and Pollen Fertility. Plant Cell, 1995, 7: 2115-2127
2. Aboul-Soud MAM, Yun B-W, Harrier LA, Loake GJ. Transformation of Fusarium oxysporum by particle bombardment and characterisation of the resulting transformants expressing a GFP transgene. Mycopathologia, 2004, 158: 475-482
3. Adams DJ, Causier BE, Mellor KJ, Keer V, Milling R, Dada J. Regulation of chitin sinthase and chitinase in fungi. In: Muzzzarelli, RAA (ed.), Chitin Enzymology. 1993, pp 15-26. European Chitin Society, Lyon, France; Ancona, Italy
4. Alagiri S, Singh TP. Stability and kinetics of a bifunctional amylase/trypsin inhibitor. Biochim. Biophys. Acta, 1993, 1203: 77-84.
5. Allaby RG, Banerjee M, Brown TA. Evolution of the high molecular weight glutenin loci of A, B, D, and G genomes of wheat. Genome, 1999,42: 296-307
6. Altabella T, Chrispeels MJ. Tobacco plants transformed with αAI gene express an inhibitor of insect a-amylase in their seeds. Plant Physiol, 1990, 93: 805-810
7. Amano M, Ogawa H, Kojima K, Kamidaira T, Suetsugu S, Yoshihama M, et al. Identification of the major allergens in wheat flour responsible for baker's asthma. Biochem J., 1998, 330: 1229-1234
8. Anderson OD, Greene FC. The characterization and comparative analysis of High-molecular-weight glutenin genes from genome A and B of a hexaploid bread wheat. Theor. Appl. Genet., 1989, 77 : 689-700
9. Aragoncillo C, Rodriguez L, Carbonero P, Garcia-Olmedo F. Chromosomal control of non-gliadin proteins from 70% ethanol extract of wheat endosperm, Theor. Appl. Genet., 1975,45:322-326
10. Armentia A, Sanchez-Monge R, Gomez L, Barber D, Salcedo G. In vivo allergenic activities of eleven purified members of a major allergen family from wheat and barley flour. Clin. Exp. Allergy, 1993, 23: 410-415
11. Arocho A, Chen BY, Ladanyi M, Pan QL. Validation of the 2 (-Delta Delta Ct) calculation as an alternate method of data analysis for quantitative PCR of BCR-ABL P210 transcripts. Diagnostic Molecular Pathology, 2006, 15: 56-61
12. Asins MJ, Carbonell EA. A comparative study on variability and phylogeny of Triticum species. Theor. Appl. Genet., 1986, 72: 551-558
13. Badaeva ED, Friebe B, Gill BS. Genome differentiation in Aegilops. 1. Distribution of highly repetitive DNA sequences on chromosomes of diploid species. Genome, 1996, 39: 293-306
14. Bai GH, Shaner G. Management of wheat and barley to Fusarium head blight. Annu. Rev. Phytopathol., 2004,42: 135-161
15. Bai GH, Shaner G. Scab of wheat: prospects for control. Plant Dis., 1994, 78: 760-765
16. Bai GH, Shaner G. Variation in Fusarium graminearum and cultivar resistance to wheat scab. Plant Dis., 1996,80: 975-979
17. Baker JE. Interaction of partially-purified amylases from larval anagasta kuehniella (lepidoptera: pyralidae) with amylase inhibitors from wheat, Comp. Biochem. PhysioL, 1989, 9311: 239-246
18. Ban T. Evaluation of resistance to Fusarium head blight in indigenous Japanese species of Agropyron (Elymus) . Euphytica, 1997, 97: 39-44.
19. Bandelt HJ, Forster P, Rohl A. Median-joining networks for inferring intraspecific phytogenies. Mol. Biol. Evol., 1999,16: 37-48
20. Barber D, Sanchez-Monge R, Mendez E, Lazaro A, Garcia-Olmedo F, Salcedo G. New and trypsin inhibitors among the CM-proteins of barley (Hordeum vulgare). Biochim. Biophys. Acta, 1986, 869: 115-118
21. Bartels D, Thompson RD. The characterization of cDNA clones coding for wheat storage proteins. Nucleic Acids Res., 1983,11: 2961-2977
22. Battershell VG, Henry RJ. High performance liquid chromatography of α-amylases from germinating wheat and complexes with the α-amylase inhibitor from barley. J. Cereal Sci., 1990, 12: 73-81
23. Baud F, Pebay-Peyroula E, Cohen-Addad C, Odani S, Lehmann MS. Crystal structure of hydrophobic protein from soybean: a member of a new cysteine-rich family. J. Mol. Biol., 1993,231:877-887
24. Baum BR, Bailey LG. The origin of the A genome donor of wheats (Triticum: Poaceae) : A perspective based on the sequence variation of the 5S DNA gene units. Genet. Resour. Crop Evol., 2004, 51: 183-196
25. Baur X, Posch A. Characterized allergens causing bakers' asthma. Allergy, 1998, 53: 562-566
26. Baur X, Weiss W, Sauer W et al. Baking agents as a contributory cause of baker's asthma. Dtsch Med Wochenschr, 1988,113: 1275-1278
27. Baur X. Baker's asthma: causes and prevention. Int Arch Occup. Environ Health., 1999, 72: 292-296
28. Bean SR, Tilley M. Sepration of water-soluble proteins from cereals by high-performance capillary electrophoresis (HPCE) . Cereal Chem., 2003, 80: 505- 510
29. Bechtel DB, Kaleikau LA, Gaines RL, Seitz LM. The effects of Fusarium graminearum infection on wheat kernels. Cereal Chem., 1985, 62: 191-197
30. Bedetti C, Bozzini A, Silano V, Luciano V. Amylase protein inhibitors and the role of Aegilops species in polyploid wheat speciation. Biochim. Biophys. Acta, 1974, 362 (2) : 299-307
31. Belton PS. On the elasticity of wheat gluten. J. Cereal Sci, 1999, 29: 103-107
32. Bernardo A, Bai G, Guo P, Xiao K, Guenzi AC, Ayoubi P. Fusarium graminearum-induced changes in gene expression between Fusarium head blight-resistant and susceptible wheat cultivars. Funct Integr Genomics, 2007, 7: 67-77
33. Bishop JG, Dean AM, Mitchell-Olds T. Rapid evolution in plant chitinases: molecular targets of selection in plant-pathogen co-evolution. Proc. Natl Acad. Sci. USA. 2000, 97: 5322-5327
34. Blake NK, Lehfeldt BR, Hemphill A, Shan X, Talbert LE. DNA sequence analysis suggests a monophyletic origin of the wheat B genome. Proc. 9th Wheat Genet. Symp. 1998,2:14-16
35. Blake NK, Lehfeldt BR, Lavin M, Talbert LE. Phylogenetic reconstruction based on low copy DNA sequence data in an allopolyploid: The B genome of wheat. Genome, 1999,42:351-360
36. Blanco A, Resta P, Simeone R, Parmar S, Shewry PR, Sabelli PW, Lafiandra D. Chromosomal location of seed storage protein genes in the genome of Daspyrum villosum (L.) Candargy. Theor. Appl. Genet, 1991, 82: 358-362
37. Blunenthal C, Gras PW, Bakes F, Barlow EWR, Wrigley CW. Possible role for the Glu-D1 locus with respect to tolerance to dough-quality change after heat stress. Cereal Chem, 1995, 72:135-136
38. Bock SA. Prospective appraisal of complaint of adverse reaction to foods in children during the first 3 yeras of life. Pediatrics, 1987, 79: 683-688
39. Boisen S, Djurtoft R. Trypsin inhibitor from rye endosperm: purification and properties. Cereal. Chem., 1981a, 58: 194-198
40. Boisen S, Djurtoft R. Trypsin inhibitor from wheat endosperm: purification and characterization. Cereal. Chem., 1981b, 58:460-463
41. Boutilier K, Hattori J, Baum BR, Miki BL. Evolution of 2S albumin seed storage protein genes in the Brassicaceae. Biochem. Syst. Evol., 1999,27: 223-234
42. Boutrot F, Guirao A, Alary R, Joudrier P, Gautier M. Wheat non-specific lipid transfer protein genes display a complex pattern of expression in developing seeds. Biochim. Biophys. Acta., 2005, 1730: 114-125
43. Brandolini A, Vaccino P, Boggini G, Ozkan H, Kilian B, Salamini F. Quantification of genetic relationships among A genomes of wheats. Genome, 2006,49: 297-305
44. Breiman A, Bogher M, Sternberg H, Graur D. Variability and uniformity of mitochondrial DNA in populations of putative diploid ancestors of common wheat. Theor. Appl. Genet., 1991, 82: 201-208
45. Breiteneder H, Ebner C. Molecular and biochemical classification of plant-derived food allergens. Journal of Allergy and Clinical Immunology, 2000,106:27-36
46. Bryant D, Moulton V. Neighbor-Net: An agglomerative method for the construction of phylogenetic networks. Mol. Biol. Evol., 2004, 21: 255-265
47. Bundock PC, Henry RJ. Single nucleotide polymorphism, haplotype diversity and recombination in the Isa gene of barley. Theor Appl Genet, 2004,109: 543-551
48. Buonocore V, Biasi MGD, Giardina P, Poreio E, Silano V. Purification and properties of an a-amylase tetrameric inhibitor from wheat kernel. Biochim. Biophys. Acta, 1985, 831:40-48
49. Buonocore V, Petrucci T, Silano V. Wheat protein inhibitors of α-amylase. Phytochemistry, 1977,16: 811-820
50. Burhenne K, Kristensen BK, Rasmussen SK. A New Class of N-Hydroxy-cinnamoyltransferases. J. Biological. Chemistry, 2003,278: 13919-13927
51. Bustos AD, Jouve N. Characterization and phylogenetic analysis of the genes coding for high molecular weight glutenin subunits in three diploid specied of Aegilops. International Journal of Plant Sciences, 2006,167: 359-366
52. Bustos AD, Rubio P, Jouve N. Molecular characterization of the inactive allele of the gene Glu-Al and the development of a set of AS-PCR markers for HMW glutenins of wheat. Theor Appl Genet, 2000,100: 1085-1094
53. Carbonero P, Garcila-Olmedo F. in Seed Proteins (Shewry PR & Casey R eds), 1999, pp. 617-633, Kluwer Academic Publishers, Dordrecht
54. Carrano L, Nitti G, Buonocore V, Caporale C, Poerio E. An effective purification procedure of amylase and trypsin inhibitors from wheat flour; isolation of a new water-soluble. Plant Scienc, 1989, 65: 25-31
55. Castagna R, Maga G, Pernzin M, Heun M, Salamini F. RFLP-based genetic relationship of Einkorn wheats. Theor. Appl. Genet., 1994, 88: 818-823
56. Chantret N, Salse J, Sabot F, Rahman S, Bellec A, Laubin B, Dubois I, Dossat C, Sourdille P, Joudrier P, Gautier M-F, Cattolico L, Beckert M, Aubourg S, Weissenbach J, Caboche M, Bernard M, Leroy P, Chalhoubb B. Molecular Basis of Evolutionary Events That Shaped the Hardness Locus in Diploid and Polyploid Wheat Species (Triticum and Aegilops) . Plant Cell, 2005, 17: 1033-1045
57. Chelkowski J, Cierniewska A, Wakuliriski W. Mycotoxins in cereal grain Part 14. Histochemical examination of fusarium-damaged wheat kernels. Nahrung, 1990, 34: 357-361
58. Chen P, Liu D, Sun W. New countermeasures of breeding for scab resistance. In: Duben HJ, Gilchrist, Reeves J and McNab A (eds) , Fusarium Head Scab: Global Status and Future Prospects, CIMMYT, Mexico DF, 1997, pp. 86-92.
59. Chen ZY, Brown LR, Lax AR, Guo BZ, Cleveland TE, Russin JS. Resistance to Aspergillus flavus in corn kernels is associated with a 14-kDa protein. Phytopathology, 1998,88:276-281
60. Cho S, Chen W, Muehlbauer FJ. Constitutive expression of the Flavanone 3-hydroxylase gene related to pathotype-specific ascochyta blight resistance in Cicer arietinum L. Physiological and Molecular Plant Pathology, 2005, 67: 100-107
61. Chrispeels MJ, Grossi-de-Sa MF, Higgins TJV. Genetic engineering with α-amylase inhibitors seeds resistant to bruchids. Seed Sci. Res., 1998, 8: 257-263
62. Christeller JT. Evolutionary mechanisms acting on proteinase inhibitor variability. FEBS Journal, 2005, 272: 5710-5722
63. Cipollini D, Enright S, Traw MB, Bergelson J. Salicylic acid inhibits jasmonic acid-induced resistance of Arabidopsis thaliana to Spodoptera exigua. Mol. Ecol., 2004,13: 1643-1653
64. Collins FS, Guyer MS. Chakravarti A Variations on a theme: Cataloging human DNA sequence variations. Science, 1997, 278: 1580-1581
65. Cooper DN, Krawczak M. The mutational spectrum of single base-pair substitutions causing human genetic disease: patterns and predictions. Hum. Genet., 1990, 85: 55-74
66. Cooper DN, Youssoufian H. The CpG dinucleotide and human genetic disease. Hum. Genet., 1988, 78: 151-155
67. Cronin JK, Bundock PC, Henry RJ, Nevo E. Adaptive climatic molecular evolution in wild barley at the Isa defense locus. Proc Natl Acad Sci U.S.A., 2007,104: 2773-2778
68. D'Egidio MG, Nardi S, Vallega V. Grain, Flour, and Dough Characteristics of Selected Strains of Diploid Wheat, Triticum monococcum L. Cereal Chemisty, 1993, 70: 298-303
69. Damude HG, Zhang H., Farrall L., Ripp KG, Tomb J-F, Hollerbach D, Yadav NS. Identification of bifunctional △12/ω3 fatty acid desaturases for improving the ratio of ω3 to ω6 fatty acids in microbes and plants. PNAS, 2006,109: 9446-9451
70. Daud HM, Gustafson JP. Molecular evidence for Triticum speltoides as a B-genome progenitor of wheat (Triticum aestivum) . Genome, 1996, 39: 543-548
71. David A. Nelsen JR. Gluten-Sensitive Enteropathy (Celiac Disease) : More Common Than You Think. American Family Physican 2002, 66: 2259-2266
72. Davis JM, Wu H, Cooke JEK, Reed JM, Luce KS, Michler CH. Pathogen Challenge, Salicylic Acid, and Jasmonic Acid Regulate Expression of Chitinase Gene Homologs in Pine. MPMI, 2002b, 15: 380-387
73. Davis P. Mills C. Shewry P. Plant Food Allergens. Biochemical Society Transactions, 2002a, 30: 901-906
74. De Bustos A , Rubio P , Jouve N. Characterization of two gene subunits on the 1R chromosome of rye as orthologs of each of the Glu-1 genes of hexaploid wheat. Theor. Appl. Genet, 2001, 76 .513-529
75. De Bustos A, Jouve N. Characterization and analysis of new HMW-glutenin alleles encoded by the Glu-R1 locus of Secale cereale. Theor. Appl. Genet., 2003, 107: 74-83
76. De Leo F, Volpicella M, Licciulli F, Liuni S, Gallerani R, Ceci LR. PLANT-PIs: a database for plant protease inhibitors and their genes, Nucleic Acids Res., 2002, 30: 347-348
77. Dempsey DA, Shah J, Klessig DF. Salicylic acid and disease resistance in plants. Crit. Rev. Plant Sci., 1999,18: 547-575
78. Deponte R, Parlamenti R, Petrucci V, Silano V, Tomasi M. Albumin α-amylase inhibitor families from wheat flour. Cereal Chem., 1976, 53: 805-820
79. Derera NF, Bhatt GM, Mcmaster GJ. On the problem of preharvest sprouting of wheat. Euphytica, 1977,26: 299-308
80. Desmyter A, Spinelli S, Payan F, Lauwereys M, Wyns L, Muyldermans S, Cambillau C. Three camelid VHH domains in complex with porcine pancreatic α-amylase. J. Biol. Chem., 2002, 277: 23645-23650
81. Dilbirligi M, Erayman M, Campbell BT, Randhawa HS, Baenziger PS, Dweikat I, Gill KS. High-density mapping and comparative analysis of agronomically important traits on wheat chromosome 3A, Genomics, 2006, 88: 74-87
82. Doares SH, Narvaez-Vasquez J, Conconi A, Ryan CA. Salicylic acid inhibits synthesis of proteinase inhibitors in tomato leaves induced by systemin and jasmonic acid. Plant. Physiol, 1995, 108: 1741-1745
83. Dong Y-C ,Zheng D-S. Genetic resource of Chinese wheat. Pekin: China agricultural publishing company, 1998
84. Douliez JP, Michon T, Elmorjani K, Marion D. Structure, biology and technological functions of lipid transfer proteins and indolines, the major lipid binding proteins from cereal kernels. J. Cereal Sci., 2000, 32: 1-20
85. D'ovidio R, Masci S, Porcedu E. Development of a set of oligonucletide primers specific for genes at the Glu-1 complex loci of wheat. Theor. Appl. Genet., 1995, 91: 189-194
86. D'ovidio R, Porceddu E, Lafiandra D, et al. PCR analysis of gene encoding allelic variants of high molecular weight glutenin subunits at the Glu-Dl locus. Theor. Appl. Genet, 1994,88:175-180
87. Dowd C, Wilson IW, McFadden H. Gene Expression Profile Changes in Cotton Root and Hypocotyl Tissues in Response to Infection with Fusarium oxysporum f. sp. Vasinfectum. MPMI, 2004, 17: 654-667
88. Dubcovsky J, Luo MC, Dvorak J. Differentiation between homoeologous chromosomes 1A of wheat and 1Am of Triticum monococcum and its recognition by the wheat Ph1 locus. Proc. Natl. Acad. Sci. U.S.A., 1995, 92: 6645-6649
89. Dvorak J, Kasarda D D, Dietler M D, et al. Chromosomal location of seed storage protein genes in the genome of Elytrugia elongata. Can.J.Genet.Cytol, 1986, 28: 818-830
90. Dvorak J, Knott DR. Disomic and ditelosomic additions of diploid Agropyron elongatum chromosomes to triticum aestivum. Can. J. Genet. Cytol., 1974, 16: 399-417
91. Dvorak J, Tetizi P, Zhang HB, Resta P. The evolution of polyploid wheats: Identification of the A genome donor species. Genome, 1993, 36: 21-31
92. Dvorak J. Homology between Agropyron elongatum chromosomes and triticum aestivum chromosomes. Can. J. Genet. Cytol., 1980,22: 237-259
93. El-Araby MM, Ahmed HFS. Differential effect of jasmonic acid on the defense of faba bean against Fusarium wilt: changes in protein and DNA patterns, peroxidase and esterase isozymes. International Journal of Agriculture and Biology, 2004, 6: 226-232
94. Engelberth J, Koch T, Schuler G, Bachmann N, Rechtenbach J, Boland W. Ion channel-forming alamethicin is a potent elicitor of volatile biosynthesis and tendril coiling. Cross talk between jasmonate and salicylate signaling in lima bean. Plant Physiol, 2001, 125: 369-377
95. Epple P, Apel K, Bohlmann H. Overexpression of an endogenous thionin enhances resistance of Arabidopsis against Fusarium oxysporum. Plant Cell, 1997, 9: 509-520
96. Erayman M, Sandhu D, Sidhu D, Dilbirligi M, Baenziger PS, Gill KS. Demarcating the gene-rich regions of the wheat genome. Nucleic. Acids Res., 32: 2004, 3546-3565
97. Erlich PR, Raven PH. Butterflies and plants: a study in co-evolution. Evolution, 1964, 1:18
98. Fahima T, Roder MS, Wendehake VM, Nevo E. Microsatellite polymorphism in antural populations of wild emmer wheat, Triticum dicocoides, in Israel. Theor. Appl. Genet, 2002,104:17-29
99. Fahima T, Sun GL, Beharav A, Krugman T, Beiles A, Nevo E. RAPD polymorphism of wild emmer wheat populations, Triticum dicocoides, in Israel. Theor. Appl. Genet., 1999,98:434-447
100.Faris J, Sirikhachornkit A, Haselkorn R, Gill B, Gornicki P. Chromosome Mapping and Phylogenetic Analysis of the Cytosolic Acetyl-CoA Carboxylase Loci in Wheat. Mol. Bio. Evol, 2001, 18: 1720-1733
101.Fedak G, Cao W, Han F, Savard M, Gilbert J, Xue A. Germplasm enhancement for FHB resistance in spring wheat through alien introgression. In: S.M. Canty, T. Boring, K. Versdahl, J. Wardwell & R.W. Ward (Eds.), Proc. 2nd Int Symp on Fusarium Head Blight, Orlando, Michigan State University, East Lansing, MI, 2004, pp. 56-57.
102.Fedak G Sources of resistance to Fusarium head blight. In: W.J. Raupp, Z. Ma, P.D. Chen & D.J. Liu (Eds.) , Proc Int Symp Wheat Improv Scab Resist, Suzhou and Nanjing, China, 2000, pp. 4.
103.Feldman M. Origin of cultivated wheat, in: A.P. Bonjean, W.J. Angus (eds.) . The world Wheat Book: A History of Wheat Breeding, Intercept Ltd, London, Paris, New York, 2000, pp. 3-56
104.Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution, 1985,39:783-791
105.Felsenstein J. PHYLIP. (Phylogeny Inference Package) version 3.6. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle. Version 3.61. 2004, available at http://evolution.gs.washington.edu/phylip.html
106.Felton GW. Nutritive quality of plant protein: sources of variation and insect herbivore response. Arch Insect Biochem Physiol, 1996, 32: 107-130.
107.Feng GH, Richardson M, Chen MS, Kramer KJ, Morgan TD, Reeck GR. α-Amylase inhibitors from wheat: a sequences and patterns of inhibition of insect and human α-amylases. Insect Biochem. Mol. Bio., 1996,26: 419-426
108.Finnie C, Melchior S, Roepstorff P, Svensson B. Proteome analysis of grain filling and seed maturation in barley. Plant Physiol., 2002, 129: 1308-1319
109.Fischer WN, Andre B, Rentsch D, Krolkiewicz S, Tegeder M, Breitkreuz K, Frommer WB. Amino acid transport in plants. Trend in Plant Science, 1998, 3: 188-195
110.Flavell RB, Goldsborough AP. Robert LS. Schnick D. Thompson RD. Genetic variation in wheat HMW glutenin subunits and the molecular basis of bread-making quality. Biotechnology, 1989, 7: 1281-1285
111.Franco OL, Ridgen DJ, Melo FR, Bloch C Jr., Silva CP, Grossi de Sa MF. Activity of wheat α-amylase inhibitors toward bruchid a-amylases and structural explanation of observed specificities. Eur. J. Biochem., 2000, 267: 2166-2173
112.Franco OL, Rigden DJ, Melo FR, Grossi-de-Sa MF. Plant a-amylase inhibitors and their interaction with insect α-amylases Structure, function and potential for crop protection. Eur. J. Biochem., 2002, 269: 397-412
113.Franken J, Stephan U, Meyer HE, Konig W. Identification of alpha-amylase inhibitor as a major allergen of wheat flour. Int. Arch. Allergy Immunol., 1994, 104: 171-174
114.Friebe B, Tuleen N, Jiang J, Gill BS. Standard karyotype of Triticum longissimum and its cytogenetic relationship with T. aestivum. Genome, 1993, 36: 731-742
115.Gale MD, Law CN, Chojecki AJ. Genetic control of a-amylase production in wheat. Theor. Appl. Genet., 1983, 64: 309-316
116.Galili S, Avivi Y, Millet E, Feldman M. RFLP based analysis of three RbcS subfamilies in diploid and polyploid species of wheat. Mol. Gen. Genet., 2000, 263: 674-680
117.Garbay B, Tautu MT, Costaglioli P. Low level of pathogenesis-related protein 1 mRNA expression in 15-day-old Arabidopsis cer6 and cer2 eceriferum mutants. Plant Science, 2007,172:299-305
118.Garcia-Casado G, Armentia A, Sanchez-Monge R, Malpica JM, Salcedo G. Rye flour allergens associated with baker's asthma. Correlation between in vivo and in vitro activities and comparison with their wheat and barley homologues. Clinical and Experimental Allergy., 1996,26: 428-435
119.Garcia-Casado G, Sanchez-Monge R, L6pez-Otin C, Salcedo G. Rye chromosome arm 3RS encodes a homodimeric inhibitor of insect α-amylase. Theor. Appl. Genet., 1994a, 89: 60-63
120.Garcia-Casado G, Sanchez-Monge R, Lopez-Otin C, Salcedo G Rye Inhibitors of Animal α-amylases Show Different Specifities, Aggregative Properties and IgE-binding Capacities than Their Homologues from Wheat and Barley European Journal of Biochemistry, 1994b, 224 (2) :525-530
121.Garcia-Maroto F, Cabonero P, Garcia-Olmedo F. Site-directed mutagenesis and expression in Escherichia coli of WMAI-1, a wheat monomeric inhibitor of insect α-amylase. Plant Mol. Biol., 1991,17:1005-1011
122.Garcia-Olmedo F, Salcedo G, Sanchez-Monge R, Gomez L, Royo J, Carbonero P. Plant proteinaceous inhibitors of proteinases and a-amylases. Oxford Surv. Plant Mol. Cell Biol., 1987,4:275-334
123.Garg K, Green P, Nickerson DA. Identification of candidate coding region single nucleotide polymorphisms in 165 human genes using assembled expressed sequence tags. Genome Research, 1999, 9: 1087-1092
124.Gatehouse AMR, Boulter D, Hilder VA. Potential of plant-derived genes in the genetic manipulation of crops for insect resistance. In Biotechnology in Agriculture, Plant Genetic Manipulation for Crop Protection. 1992, Vol. 7, pp. 155-181. CAB International, Wallingford, UK.
125.Gatehouse AMR, Gatehouse JA. Identifying proteins with insecticidal activity: use of encoding genes to produce insect-resistant transgenic crops. Pest Sci., 1998, 52: 165-175
126.Gianibelli MC, Solomon RG. A novel y-type high Mr glutenin subunit (12.4~t) present in Triticum tauschii. J. Cereal Sci., 2003, 37: 253-256.
127.Gilbert J, Tekauz A. Review: Recent developments in research on Fusarium head blight of wheat in Canada. Can. J. Plant Pathol., 2000,22: 1-8
128.Golkari S, Gilbert J, Parshar S, Procunier JD. Microarray analysis of Fusarium graminearum-induced wheat genes, identification of organ-specific and differentially expressed genes. Plant Biotechnology Journal, 2007, 5: 38-49
129.Gomez L, Sanchez-Monge R, Lopez-Otin C, et al. Wheat inhibitors of heterologous α-amylases. Plant Physiol., 1991, 96: 768-774
130.Gorinstein S, Zemser M, Fliess A, Shnitman I, Paredes-Lopez O, Yamamoto K, Kobayashi S, Taniguchi H. Computational analysis of the amino acid residue sequences of amaranth and some other proteins. Biosci. Biotechnol. Biochem., 1998, 62: 1845-1851
131.Gu YQ, Coleman-Derr D, Kong X, Anderson OD. Rapid genome evolution revealed by comparative sequence analysis of orthologous region from four Triticeae genomes. Plant Physiology, 2004a, 135: 459-470
132.Gu YQ, Crossman C, Kong X, Luo MC, You FM, Coleman-Derr D, Dubcovsky J, Anderson OD. Genomic organization of the complex α-gliadin gene loci in wheat, Theor. Appl. Genet., 2004b, 109: 648-657
133.Guo ZF, Yan ZH, Wang JR, Wei YM, Zheng YL. Characterization of HMW prolamines and their coding sequences from Crithopsis delileana. Hereditas, 2005, 142:56-64
134.Gupta RB, Khan K, MacRitchie F. Biochemical basis of flour properties in bread wheats. I . Effects of variation in the quantity and size distribution of polymeric protein. J. Cereal Sci., 1993,18: 23-41
135.Gupta RB, Popineau Y, Lefebvre J, Cornect M, Lawrence GJ, MacRitchie F. Biochemical basis of flour properties in bread wheats. Ⅱ. Changes in polymeric protein formation and dough/gluten properties associated with the loss of low Mr or high Mr glutenin subunits. J. Cereal Sci., 1995, 21: 103-116
136.Gupta RB, Shepherd KW, MacRitchie F. Genetic control and biochemical properties of some high molecular weight albumins in bread wheat. Journal of Cereal Science, 1991,13:221-235
137.Gupta RB, Sphepherd KW. Production of multiple wheat-rye 1RS translocation stocks and genetic analysis of LMW subunits of glutenin and gliadins in wheat using these stocks. Theor. Appl. Genet., 1993, 85:719-728
138.Halford NG, Forde J, Anderson OD, Greene FC, Shewry PR. The nucleotide and deduced amino-acid sequences of an HMWglutenin subunit gene from chromosome 1B of bread wheat (Triticum aestivum L.) and comparison with those of genes from chromosomes 1A and 1D. Theor. Appl. Genet, 1987,75:117-126
139.Hammer K, Filatenko AA, Korzun V. Microsatellite markers: A new tool for distinguishing diploid wheat species. Genet. Resour. Crop Evol., 2000,47: 497-505
140.Han FP, Fedak G Molecular characterization of partial amphiploids from Triticum durum x tetraploid Thinopyrum elongatum as novel source of resistance to wheat Fusarium head blight. In: Proceeding of the 10th International Wheat Genetics Symposium, 2003, Paestum Italy. Edited by Pogna NE et al. pp 1148-1150
141.Hanson DG, Vaz N, Maia L, et al. Inhibition of specific immune response by feeding protein antigen. Int. Arch Allergy Appl. Immunol, 1977, 55: 1518-1524
142.Harding RM, Fullerton SM, Griffiths RC, Bond J, Cox MJ, Schneider JA, Moulin DS, Clegg JB. Archaic African and Asian lineages in the genetic ancestry of modern humans. Am. J. Hum. Genet., 1997, 60: 772-789
143.Harlan JR, Zohary D. Distribution of wild wheat and barley. Science, 1966, 153: 1074-1080
144.Hart LP, Pestka JJ, Liu MF. Efect of kenel development and period on production of deoxynivalenol in wheat infected with Gibberella zeae. Phytopathology, 1984, 74: 1415-1418
145.Hejgaard J, Bjorn SE, Nielson G Rye chromosomes carrying structure genes for the major grain protease inhibitor. Hereditas, 1984, 101: 257-259
146.Hilder V, Gatehouse A, Sheerman S, Barker R, Boulter D. A novelmechanismof insect resistance engineered into tobacco. Nature, 1987, 330: 160-163
147.Hillis DM, Bull JJ. An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis. Systematic Biology, 1993,42: 182-192
148.Himer A, Ladwig F, Stransky H, Okumoto S, Keinath M, Harms A, Frommer WB, Koch W. Arabidopsis LHT1 Is a High-Affinity Transporter for Cellular Amino Acid Uptake in Both Root Epidermis and Leaf Mesophyll. Plant Cell, 2006, 18: 1931-1946
149.Hoffmann L, Maury S, Martz F, Geofrroy P, Legrand M. Purification, cloning, and properties of an acyltransferase controlling shikimate and quinate ester intermediates in phenylpropanoid metabolism. J. Biol. Chem., 2003,278: 95-103
150.Holliday R, Girgg WG DNA methylation mutation. Mutation Res., 1993,285: 61-67
151.Holliday RA. Mechanism for gene conversion in fungi. Genet. Res., 1964, 5: 282-304
152.Houba R, Doekes G, Heederik D. Occupational respiratory allergy in bakery workers: a review of the literature. Am. J. Respir. Crit. Care. Med., 1998, 158: 1499-1503
153.Hsiao C, Chatterton NJ, Asay KH. Phylogenetic relationships of the monogenomic species of the wheat tribe, Triticeae (Poaceae) , inferred from nuclear rDNA (internal transcribed spacer) sequences. Genome, 1995, 38: 211-231
154.Huang S, Sirikhachornkit A, Fans JD, Su X, Gill BS, Haselkorn R, Gornicki P. Phylogenetic analysis of the acetyl-CoA carboxylase and 3-phosphoglycerate kinase loci in wheat and other grasses. Plant Mol. Biol., 2002b, 48: 805-820
155.Huang S, Sirikhachornkit A, Su X, Faris J, Gill B, Haselkorn R, Gornicki P. Genes encoding plastid acetyl-CoA carboxylase and 3-phosphoglycerate kinase of the Triticum/Aegilops complex and the evolutionary history of polyploid wheat, Proc. Natl. Acad. Sci. USA. 2002a, 99: 8133-8138
156.Huebner FR, Bietz JA. Improvements in wheat protein analysis and quality prediction by reversed phase high performance liquid chromatography. cereal chemistry, 1987. 64: 15-20
157.Huelsenbeck JP, Ronquist F. MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics, 2001,17: 754-755
158.Hughes DA, Mills C. Food allergy: a problem on the increase. Biologist (London) , 2001,48:201-204
159.Husby S, Mestecky J, Moldozeanu Z, et al. Oral tolerance in humans T cell but not B cell tolerance after antigen feeding. J. Immunol., 1994, 152: 4463-4470
160.Huson DH, Bryant D. User manual for SplitsTree 4. 2005, available at http ://www. splits tree. org
161.Irizarry RA, Hobbs B, Collin F, Beazer-Barclay YD, Antonellis KJ, Scherf U. Speed TP. Exploration, normalization and summaries of high density oligonucleotide array probe level data. Biostatistics, 2002, 4: 249-264
162.Iulek J, Franco OL, Silva M, Slivinski CT, Bloch C Jr., Rigden DJ, Grossi de Sa MR Purification, biochemical characterization and partial primary structure of a new α-amylase inhibitor from Secale cereale (rye) . Int. J. Biochem. Cell Biol., 2000, 32: 1195-1204
163.Jackowiak H, Packa D, Wiwart M, Perkowski J, Busko M, Borusiewicz A. Scanning electron microscopy of mature wheat kernels infected with Fusarium culmorum. J. Appl. Genet., 2002,43A: 167-176
164.Jarrett SJ, Marschke RJ, Symons MH, Gibson CE, Fox GP, Henry RJ. Alpha-amylase/subtilisin inhibitor levels in Australian barleys. J. Cereal Sci., 1997,25: 261-266
165.Jauhar PP, Peterson TS. Hybrids between durum wheat and Thinopyrum junceiforme: Prospects for breeding for scab resistance. Euphytica, 2001, 118: 127-136
166.Jiang J, Gill BS. Different species-specific chromosome translocations in Triticum timopheevi and Triticum turgidum support the diphyletic origin of polyploid wheats. Chromo Research, 1994,2: 59-64
167.Jiang QT, Wei YM, Wang JR, Yan ZH, Zheng YL. Isolation and Sequence Analysis of HMW Glutenin Subunit 1Dy10.1 Ecoding Gene from Xinjiang Wheat (Triticum petropavlovskyi Udacz. et Migusch). Agricultral Science in China, 2006, 5(2): 81-89
168.Jobb G. TreeFinder Version of October 2005. Munich, Germany. Distributed by the author at http://www.treefinder.de
169.Johnson BL, Dhaliwal HS. Reproduction isolation of Triticum boeoticum and Triticum urartu and the origin of the tetraploid wheats. Am. J. Bot., 1976, 63: 1088-1094
170.Jouanin L, Bonade-Bottino M, Girard C, Morrot G, Giband M. Transgenic plants for insect resistance. Plant Sci., 1998,131:1-11
171.Kang Z, Buchenauer H. Ultrastructural and immunocytochemical investigation of pathogen development and host responses in resistant and susceptible wheat spikes infected by Fusarium culmorum. Physiol. Mol. Plant Pathol., 2000b, 57: 255-268
172.Kashkush K, Feldman M, Levy AA. Gene loss, silencing and activation in a newly synthesized wheat allotetraploid. Genetics, 2002,160: 1651-1659
173.Kashkush K, Feldman M, Levy AA. Transcriptional activation of retrotransposons alters the expression of adjacent genes in wheat. Nature Genetics, 2003, 33: 102-106
174.Keck B, Kohler P, Wieser H. Disulfate bonds in wheat gluten: cystine peptides derived from gluten proteins following peptic and thermolytic digestion. Z. Lebensm.Unters. Forsch 1995, 200: 432-439
175.Kihara H. Wheat studies-restrospect and prospects. Crop Science, 1982, 40: 1742-1749
176.Kohler P, Belitz H-D, Wieser H. Disulfate bonds in wheat gluten: further cysteine peptides from high molecular weight (HMW) and low molecular weight (LMW) subunits of glutenin and γ-gliadins. Z Lebensm Unters Forsch, 1993, 196: 239-247
177.Kolattukudy PE, Rogers LM, Li D, Hwang C-S, Flaishman MA. Surface signalling in pathogenesis. Proc. Natl. Acad. Sci. USA, 1995, 92: 4080-4087
178.Kolderup F. Effects of temperature, photoperiod and light quantity on protein production in wheat grains. J. Sci. Food Agric. 1975, 26: 583-592
179.Konarev AV. Interaction of insect digestive enzymes with plant protein inhibitors and host-parasite co-evolution. Euphytica, 1996, 92: 89-94
180.Kreis M, Shewry PR, Forde BG, Forde J, Miflin BJ. Structure and evolution of seed storage proteins and their genes with particular reference to those of wheat, barley and rye. Oxf Surv Plant Mol Cell Biol, 1985,2: 253-317
181.Kumar S, Tamura K, Nei M. MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform, 2004, 5: 150-163
182.Lankisch M, Layer P, Rizza RA. DiMagno EP. Acute Postprandial Gastrointestinal and Metabolic Effects of Wheat Amylase Inhibitor (WAI) in Normal, Obese, and Diabetic Humans. Pancreas, 1998, 17 (2) : 176-181
183.Larsson S. Stressful times for the plant stress-insect performance hypothesis. Oikos, 1989,56:277-283.
184.Lawrence GJ, Shepherd KW. Chromosomal location of genes controlling seed proteins in species related to wheat. Theor. Appl. Genet., 1981, 59: 25-31
185.LeCorre V, Bernard M. Assessment of the type and degree of restriction fragment length polymorphism (RFLP) in diploid species of the genus Triticum. Theor. Appl. Genet, 1995,90: 1063-1067
186.Lehtio J, Teeri TT, Nygren P-A. Alpha-amylase inhibitors selected from a combinatorial library of a cellulose binding domain scaffold. Proteins, 2000, 41: 316-322
187.Lerche MH, Poulsen FM. Solution structure of barley lipid transfer protein complexed with palmitate. Two different binding modes of palmitate in the homologous maize and barley nonspecific lipid transfer proteins. Protein Sci., 1998, 7: 2490-2498
188.Levy AA, Feldman M. The impact of polyploidy on grass genome evolution. Plant Physiology, 2002, 130: 1587-1593
189.Lew EJL, Kuzmicky DD, Kasarda DD. Characterization of low molecular weight glutenin subunits by reversed-phase high-performance liquid chromatography, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and N-terminal amino acid sequencing. Cereal Chem, 1992, 69: 508-515
190.Lindsay MP, Tamas L, Appels R, Skerritt JH. Direct evidence that the number and location of cysteine residues affect glutenin polymer structure. J. Cereal Sci., 2000, 31: 321-333
191.Liu B, Segal G, Rong JK, Feldman M. A chromosome-specific sequence common to the B genome of polyploid wheat and Aegilops searsii. Plant Syst. Evol., 2003b, 241: 55-66
192.Liu Z, Yan Z, Wan Y, Liu K , Zheng Y, Wang D. Analysis of HMW glutenin subunits and their coding sequences in two dipliod Aegilops species. Theor Appl Genet, 2003a, 106:1368-1378
193.Lorito HJM, Brederode FT, Hayes CK, Woo SL, Noviello C, Williamas DL, Harman GE. Proteinase inhibitors from plants as a novel class of fungicides. Mol. Plant-Microbe Interact., 1994, 7: 525-527 .
194.Louda SM, Collinge SK. Plant resistance to insect herbivores: a field test of the environmental stress hypothesis. Ecology 1992, 73:153-169.
195.Lowney E. Suppression of contact sensirization in man by prior feeding of antigen. J Invest Dermatol, 1973, 61: 90-93
196.Lu S, Deng P, Liu X, Luo J, Han R, Gu X, Liang S, Wang X, Li F, Lozanov V, Patthy A, Pongor S. Solution structure of the major α-amylase inhibitor of the crop plant amaranth. J. Biol. Chem., 1999,274: 20473-20478
197.Luo MC, Yang ZL, Kota RS, Dvorak J. Recombination of chromosomes 3Am and 5 Am of Triticum monococcum with homoeologous chromosomes 3A and 5A of wheat: The distribution of recombination across chromosomes. Genetics, 2000, 154: 1301-1308
198.Lyons A, Richardson M, Tatham AS, Shewry PR. Characterization of homologous inhibitors of trypsin and the a-amylase. Biochim. Biophys. Acta, 1987, 915: 305-313
199.MacGregor EA, Bazin SL, Ens EW, Lahnstein J, Macri LJ, Shirley NJ, MacGregor AW. Structural models of limit dextrinase inhibitors from barley. J. Cereal Sci., 2000, 31:79-90
200.MacGregor EA, Janecek S, Svensson B. Relationship of sequence and structure to specificity in the a-amylase family of enzymes. Biochim. Biophys. Acta, 2001, 1546: 1-20
201.Mackie AM, Lagudah ES, Sharp PJ. Molecular and biochemical characterization of HMW glutenin subunits from T. tauschii and the D genome of hexapoid wheat . J. Cereal Sci., 1996,23:213-225
202.Maddison WP, Maddison DR. Mesquite: a modular system for evolutionary analysis. Version 1.06, 2005, available at http://mesquiteproject.org
203.Maeda K, Kakabayashi S, Matubara H. Complete amino acid sequence of an α-amylase inhibitor in wheat kernel (0.19-inhibitor) . Biochim. Biophys. Acta, 1985, 828:213-221
204.Maeda K. The complete amino-acid sequence of the endogenous a-amylase inhibitor in wheat. Biochem. Biophys. Acta, 1983, 871: 250-256
205.Maestra B, Naranjo T. Homoeologous relationships of Aegilops speltoides chromosomes to bread wheat. Theor. Appl. Genet, 1998, 97: 181-186
206.Maestra B, Naranjo T. Homoeologous relationships of Triticum sharonense chromosomes to T. aestivum. Theor. Appl. Genet., 1997, 94: 657-663
207.Mandal S. Mandal RK. Seed storage proteins and approaches for improvement of their nutritional quality by genetic engineering. Current Science, 2000, 79: 576-589
208.Marth GT, Korf I, Yandell MD, Yeh RT, Gu Z, Zakeri H, Stiziel NO, Hillier L, Kwok PY, Gish WR. A general approach to single-nucleotide polymorphism discovery. Nature Genet., 1999, 23: 452-456
209.Maruyama N, Ichise K, Katsube T, Kishimoto T, Kawase S, Matsumura Y, et al. Identification of major wheat allergens by means of the Escherichia coli expression system. Eur. J. Biochem, 1998,255: 739-745
210.Masojc P, Zawitowski J, Howes NK. Polymorphim and chromosomal location of endogenousa-amylase inhibitor genes in common wheat. Theor Appl. Genet., 1993, 85: 1043-1048
211.Matsuo H, Kohno K, Niihara H, Morita E. Specific IgE Determination to Epitope Peptides of ω-Gliadin and High Molecular Weight Glutenin Subunit Is a Useful Tool for Diagnosis of Wheat-Dependent Exercise-Induced Anaphylaxis. The Journal of Immunology, 2005, 175: 8116-8122
212.Matsuo H, Morita E, Tatham AS, Morimoto K, Horikawa T, Osuna H, Ikezawa Z, Kaneko S, Kohno K, Dekio S. Identification of the IgE-binding epitope in γ-5 gliadin, a major allergen in wheat-dependent exerciseinduced anaphylaxis. J. Biol. Chem., 2004,279: 12135-12140
213.Mattson WJ, Haack RA. The role of drought in outbreaks of plant-eating insects. BioScience, 1987, 37: 110-118
214.McEvoy PB. Insect-plant interactions on a planet of weeds. Entomologia Experimentalis et Applicata, 2002, 104: 165-179
215.McMullen M, Jones R, Gallenberg D. Scab of wheat and barley: A re-emerging disease of devastating impact. Plant Dis., 1997, 81: 1340-1348
216.Mena M, Sanchez-Monge R, Gomez L, Salcedo G. Carbonero P. Arnajor barley allergen associated with baker's asthma disease is a glycosylated monomeric inhibitor of insect alpha-amylase: cDNA cloning and chromosomal location of the gene. Plant Mol.Biol., 1992,20:451-458
217.Mentewab A, Rezanoor HN, Gosman N, Worland AJ, Nicholson P. Chromosomal location of Fusarium head blight resistance genes and analysis of the relationship between resistance to head blight and brown foot rot. Plant Breed., 2000, 119: 15-20
218.Mesterhazy A. Types and components of resistance to Fusarium head blight of wheat. Plant Breed, 1995, 114: 337-386
219.Metakovsky EV, Baboev SK. Polymorphism of gliadin and unusual gliadin alleles in Triticum boeoticum. Genome, 1992,35: 1007-1012
220.Miedaner T. Review. Breeding wheat and rye for resistance to Fusarium diseases. Plant Breed., 1997, 116: 201-220
221.Miller T E. Systematicas and evolution. In: Wheat Breeding-its scientific basis. Edited by Lupton F G, Chapman and Hall Ltd. 1987, 1-28
222.Millerd A. Biochemistry of legume seed proteins. Ann. Rev. Plant Physiol., 1975, 26: 53-72
223.Miyashita NT, Mori N, Tsunewaki K. Molecular variation in chloroplast DNA regions in ancestral species of wheat. Genetics, 1994,137: 883-889
224.Moghaddam M, Ehdaie B, Waines JG. Genetic diversity in populations of wild wheat Triticum urartu Tum.ex.Gandil. revealed by isozyme markers. Genetic Resources and Crop Evolution, 2000,47: 323-334
225.Molodchenkova OO, Adamovskaya VG, Levitskii YA, Gontarenko OV, Sokolov VM. Maize Response to Salicylic Acid and Fusarium moniliforme. Applied Biochemistry and Microbiology, 2002, 38: 381-385
226.Montebove L, De Pace C, Jan CC, Scarascia Mugnozza GT, Qualset CO. Chromosomal location of isozyme and seed storage protein genes in Dasypyrum villosum (L.) Candargy. Theor. Appl. Genet, 1987, 73: 836-845
227.Morita E, Matsuo H, Mihara S, Morimoto K, Savage AW, Tatham AS. Fast γ-gliadin is a-major allergen in wheat-dependent exercise-induced anaphylaxis. J. Dermatol. Sci, 2003, 33:99-104
228.Morris LD, Raupp WJ, Gill BS. Isolation of Ht genome chromosome additions from polyploid Elymus trachycaulus (StStHtHt) into common wheat (Triticum aestivum) . Genome, 1990,33: 16-22
229.Mundy J, Hejgaard J, Svendsen I. Characterization of a bifunctional wheat inhibitor of endogenous α-amylase and subtilisin. FEBS Lett, 1984, 167: 210-214
230.Mundy J, Svendsen I, Heigaard J. Barley α-amylase/subtilisin inhibitor. I . Isolation and characterization. Carlsberg Res. Commun., 1983,48: 81-90
231.Murray M, Thompson WF. Rapid isolation of high molecular weight plant DNA. Nucleic. Acids Res., 1980, 8: 4321-4325
232.Mustalahti K, Lohiniemi S, Collin P, Vuolteenaho N, Laippala P. Gluten-Free Diet and Quality of Life in Patients with Screen-Detected Celiac Disease. Effective Clinical Practice, 2002, 5: 105-113
233.Nagasue A, Fukamachi H, Ikenaga H, Funatsu G. The amino acid sequence of barley rootlet trypsin inhibitor. Agric. Biol. Chem., 1988, 52: 1505-1514
234.Naranjo T. Chromosome structure of Triticum longissimum relative to wh(?)at. Theor. Appl. Genet., 1995,91: 105-109
235.Nawrath C, Heck S, Parinthawong N, Metraux J-P. EDS5, an essential component of salicylic acid-dependent signaling for disease resistance in Arabidopsis, is a member of the MATE transporter family. Plant Cell, 2002, 14: 275-286
236.Nelson JC, Van Deynez AE, Autrique E, Sorrells ME, Lu YH, Negre S, Bernard M, Leroy P. Molecular mapping of wheat: homoeologous group 3. Genome, 1995, 38: 525-533
237.Nevo E, Beiles A, Krugman T. Natural selection of allozyme polymorphisms: a microgeographic climatic differentiation in wild emmer wheat (Triticum dicoccoides). Theor. Appl. Genet., 1988b, 75: 529-538
238.Nevo E, Beiles A, Krugman T. Natural selection of allozyme polymorphisms: a microgeographical differentiation by edaphic, topographical, and temporal factors in wild emmer wheat (Triticum dicoccoides) . Theor. Appl. Genet., 1988c, 76:737-752
239.Nevo E, Beiles A. Genetic diversity of wild emmer wheat in Israel and Turkey: Structure, evolution and application in breeding. Theor. Appl. Genet., 1989, 77: 421-455
240.Nevo E, Carver BF, Beiles A. Photosynthetic performance in wild emmer wheat, Triticum dicoccoides: ecological and genetic predictability. Theor. Appl. Genet., 1991b, 81:445-460
241.Nevo E, Golenberg E, Beiles A. Genetic diversity and environmental associations of wild wheat, Triticum dicoccoides, in Israel. Theor. Appl. Genet., 1982,62: 241-254
242.Nevo E, Nishikawa K, Furuta Y, Gonokami Y, Beiles A. Genetic polymorphisms of a- and β-amylase in wild emmer wheat, Triticum dicoccoides, in Israel. Theor. Appl. Genet, 1993,85: 1029-1042
243.Nevo E, Noy-Meir I, Beiles A, Krugman T, Agami M. Natural selection of allozyme polymorphisms: mocro-geographical spatial and temporal ecological differentiations in wild emmer wheat. Israel J. Botany, 1991a, 40: 419-449
244.Nevo E, Pagnotta MA, Beiles A, Porceddu E. Wheat storage proteins: glutenin diversity in wild emmer, Triticum dicoccoides, in Israel and Turkey. 3. Environmental correlates and allozymic associations. Theor. Appl. Genet., 1995, 91: 415-420
245.Nevo E, Payne PI. Wheat storage proteins: glutenin diversity in wild emmer, Triticum dicoccoides, in Israel and Turkey. 1. Geographical patterns and ecological predictability. Theor. Appl. Genet., 1987, 74: 827-836
246.Nevo E. Beiles A. and Storch N. 1983.Microgeographic edaphic differentiation in hordein polymorphisms of wild barley.Theor .Appl.Genet, 64:123 132
247.Nevo E. Evolution of genome-phenome diversity under environmental stress. Proc. Natl. Acad. Sci. U.S.A., 2001, 98: 6233-6240
248.Nevo E. Genetic diversity in nature: patterns and theory. Evol. Biol., 1988a, 23: 217-246
249.Nevo E. Molecular evolution and ecological stress at global, regional and local scales: the Israeli perspective. J. Exp. Zool, 1998,282: 95-119
250.Newton CR, Graham LE, Heptinstall LE, Powell SJ, Summers C, Kalsheker N, Smith JC, Markham AF. Analysis of any point mutation in DNA. The amplification refractory mutation system (ARMS) . Nucleic Acids Research, 1989,17: 2503-2516
251.Nickerson DA, Taylor SL, Weiss KM, et al. DNA sequence diversity in a 9.7 kb region of the human lipoprotein lipase gene. Nature Genetics, 1998,19: 233-240
252.Nightingale MJ, Marchylo BA, Clear RM, Dexter JE, Preston KR. Fusarium head blight: effect of fungal proteases on wheat storage proteins. Cereal Chem., 1999, 76: 150-158
253.Nishiuchi T., Masuda D., Nakashita H., Ichimura K., Shinozaki K., Yoshida S., Kimura M., Yamaguchi I., Yamaguchi K. Fusarium phytotoxin trichothecenes have an elicitor-like activity in arabidopsis thaliana, but the activity differed significantly among their molecular species. MPMI 2006,19: 512-520
254.Oda Y, Matsunaga T, Fukuyama K, Miyazaki T, Morimoto T. Tertiary and quaternary structures of 0.19 alpha-amylase inhibitor from wheat kernel determined by X-ray analysis at 2.06 A resolution. Biochemistry, 1997, 36 (44) : 13503-13511
255.Ogihara Y, Tsunewaki K. Diversity and evolution of chloroplast DNA in Triticum and Aegilops as revealed by restriction fragment analysis. Theor. Appl. Genet., 1988, 76: 321-332
256.Okuda M, Satoh T, Sakurai N, Shibuya K, Kaji H, Samejima T. Overexpression in Escherichia coli of chemically synthesized gene for active 0.19 alpha-amylase inhibitor from wheat kernel. J. Biochemistry, 1997, 122 (5) : 918-926
257.Oliver PE, Cai X, Xu SS, Chen X, Stack RW. Wheat-Alien Species Derivatives: A Novel Source of Resistance to Fusarium Head Blight in Wheat. Crop Sci. 2005, 45: 1353-1360
258.Oliver R, Osbourn A. Molecular dissection of fungal phytopathogenicity. Microbiol., 1995, 141: 1-9
259.Olivera PE, Steffenson B, Anikster Y. Reaction of Aegilops sharonesis to Fusarium head blight. In: S.C. Canty, J. Lewis & R.W. Ward (Eds.) , 2003 National Fusarium Head Blight Forum Proc, U.S. Wheat & Barley Scab Initiative, 2003, pp. 226 (abstract) .
260.Orth RA, Bushuk W. A comparative study of the proteins of wheat of diverse baking quality. Cereal Chem., 1972,49: 268-275.
261.Orth RA, Bushuk W. Studies on glutenin: 11 Relation of variety location of growth and baking quality to molecular weight distribution of subunits.Cereal Chem., 1973, 50: 191-197
262.Osbeme TB. The protein of the wheat kernel. Mashington: publication of the Carnegie institute, 1907
263.Pagnotta MA, Nevo E, Beiles A, Porceddu E. Wheat storage proteins: glutenin diversity in wild emmer, Triticum dicoccoides, in Israel and Turkey. 2. DNA diversity detected by PCR. Theor. Appl. Genet., 1995, 91: 409-414.
264.Pandya MJ, Sessions RB, Williams PB, Dempsey CE, Tatham AS, Shewry PR, Clarke AR. Structural characterization of a methionine-rich, emulsifying protein from sunflower seed. Proteins, 2000, 38 (3) : 341-349
265.Parry DW, Jenkinson P, McLeod L. Fusarium ear blight (scab) in small grain cereals- a review. Plant Pathol., 1995, 44: 207-238
266.Payan F. Structural basis for the inhibition of mammalian and insect α-amylase by plant inhibitors. Biochim. Biophys. Acta, 2004,1696: 171-180
267.Payne PI, Corfield KG. Subunit composition of wheat glutenin proteins isolated by gel filtration in a dissociating medium. Planta, 1979,145: 83-33
268.Payne PI, Lawrence GJ. Catalogue of alleles for the complex gene loci, Glu-A1, Glu-B1 and Glu-D\ which code for high molecular weight subunits of glutenin in hexaploid wheat. Cereal Res Commun, 1983,11: 29-35
269.Payne PI. Genetics of wheat storage proteins and the effect of allelic variation on breadmaking quality. Annu Rev Plant Physiol., 1987b, 38: 141-153
270.Payne PL The relationship between HMW glutein subunit composition and the bread-making quality of British-grown wheat varieties. J. Sci. Food Agric, 1987a, 40: 51-65
271.Pekkarinen AI, Jones BL. Trypsin-like proteinase produced by Fusarium culmorum grown on grain proteins. J. Agric. Food Chem., 2002, 50: 3849-3855
272.Peraica M, Radic B, Lucic A, Pavlovic M. Toxic effects of mycotoxins in humans. Bull. World Health Org., 1999, 77: 754-766
273.Petersen G, Seberg O, Yde M, Berthelsen K. Phylogenetic relationships of Triticum and Aegilops and evidence for the origin of the A, B, and D genomes of common wheat (Triticum aestivum) . Mol. Phyl. Evol., 2006, 39: 70-82
274.Petrucci T, Rab A, Tomasi M, Silano V. Further characterization studies of the α-amylase protein inhibitor of gel electrophoretic mobility 0.19 from wheat kernel. Biochim. Biophys. Acta, 1976,420: 288-297
275.Petrucci T, Sannia G, Parlamenti R, et al. Structural studies of wheat monomeric and dimeric protein inhibitors of alph a-amylase. Biochem J., 1978, 173: 229-235
276.Petrucci T, Tomasi M, Cantagalli P, Silano V. Comparison of wheat albumin inhibitors of a-amylase inhibitors and trypsin. Phytochemistry, 1974,13: 2487-2495
277.Pfeil T, Schwabl U, Ulmer WT, Konig W. Western blot analysis of water-soluble wheat flour (Triticum vulgaris) allergens. Int. Allergy. Appl. Immunol., 1990, 91: 224-231
278.Picoult-Newberg L, Ideker TE, Pohl MG, Taylor SL, Donaldson MA, Nickerson DA, Boyce-Jacino M. Mining SNPs from EST databases, Genome Research, 1999, 9: 167-174
279.Placinta CM, D'Mello JPF, Macdonald AMC. A review of worldwide contamination of cereal grains and animal feed with Fusarium mycotoxins. Anim. Feed Sci. Technol., 1999,78:21-37
280.Poerio E, Caporale C, Carrano L, Pucci P, Buonocore V. Assignment of the five disulphide bridges in an alphα-amylase inhibitor from wheat kernel by FAB/MS and Edman degradation. Eur. J. Biochem., 1991, 199: 595-600
281.Posada D, Crandall KA. Modeltest: Testing the model of DNA substitution. Bioinformatics, 1998, 14: 817-818
282.Posch A, Weiss W, Wheeler C, Dunn MJ, Gorg A. Sequence analysis of wheat grain allergens separated by two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis, 1995,16: 1115-1119
283.Prakash B, Selvaraj S, Murthy MRN, Sreerama YN, Rajagopal RD, Gowda LR. Analysis of the amino acid sequences of plant Bowman-Birk inhibitors. J. Mol. Evol., 1996,42:560-569
284.Price J, Root T. Ecosystem Functioning Under Abrupt Climate Change, Potential Surprises and Adaptations. 2004, [http://www.accstrategy.org/presentdrafts.html]
285.Pritsch C, Vance CP, Bushnell WR, Somers DA, Hohn TM, Muehlbauer GJ. Systemic expression of defense response genes in wheat spikes as a response to Fusarium graminearum infection. Physiol. Mol. Plant Pathol., 2001, 58: 1-12
286.Rafalski A. Aplications of single nucleotide polymorphisms in crop genetics, Plant Biology, 2002a, 5:94-100
287.Rafalski A. Novel genetic mapping tools in plants: SNPs and LD-based approaches. Plant Sci., 2002b, 162: 329-333
288.Rahman S, Jolly CJ, Skerritt JH, Wallosheck A. Cloning of a wheat 15 kDa grain softness protein (GSP) . GSP is a mixture of puroindoline-like polypeptides. Eur. J. Biochem., 1994,223:917-925
289.Rambaut A, Drummond A. 2005, Tracer, available at http://evolve.zoo.ox.ac.uk
290.Raskina O, Belyyev A, Nevo E. Repetitive DNAs of wide emmer wheat Triticum dicocoides and their relation to S-genome species: molecular-cytogenetic analysis. Genome, 2002,45 (2) : 391-401
291.Reddy P, Appels R. Analysis of a genomic DNA segment carrying the high-molecular wheat (HMW) glutenin Bx 17 subunit and its use as an RFLP marker. Theor. Appl. Genet., 1993, 85: 616-624
292.Remington DL, Thornsberry JM, Matsuoka Y, Wilson LM, Whitt SR, Doebley J, Kresovich S, Goodman MM, Buckler ES. Structure of linkage disequilibrium and phenotypic associations in the maize genome.Proc Natl Acad Sci U.S.A.,2001,98:11479-11484
293.Riichiro U,Ayako N,Shoichi T,Kouichi K,Koichiro T,Nobuyuki Y.New enzymatic synthesis of 63-modified maltooligosaccharides and their inhibitory activities for human α-amylases.Carbohydrate Research,1998,307:69-76
294.Ryals JA,Neuenschwander UH,Willits MG,Molina A,Steiner H-Y,Hunt MD.Systemic acquired resistance.Plant Cell,1996,8:1809-1819
295.Ryan CA,Pearce G.Systemin:a polypeptide signal for plant defensive genes.Annu.Rev.Cell Dev.Biol.,1998,14:1-17
296.Ryan CA.Protease inhibitors in plants:genes for improving defenses against insect and pathogens.Annu.Rev.Phytophathol,1990,28:425-449
297.Ryan CA.The systemin signaling pathway:differential activation of plant defensive genes.Biochim.Biophys.Acta,2000,1477:112-121
298.Sales MP,Gerhardt IR,Grossi-de-SaA MF,Xavier-Filho J.Do legumes storage proteins play a role in defending seeds against bruchids? Plant Physiol.,2000,124,515-522
299.Sampson HA.Food allergy.I.Immunopathogenesis and clinical disorders.J.Allergy Clin.Immunol.,1999,103:717-728
300.Sanchez-Monge R,Barber D,Mendez E,Garcia-Olmedo F,Salcedo G.Gene encoding alpha-amylase inhibitors are located in the short arms of Chromosomes 3B,3D and 6D of wheat(Triticum aestivum L.).Theor.Appl.Genet.,1986,72:108-113
301.Sanchez-Monge R,Garcia-Casado G,Lopez-Otin C,Armentia A,Salcedo G.Wheat flour peroxidase is a prominent allergen associated with baker's asthma.Clin.Exp.Allergy,1997,27:1130-1137
302.Sanchez-Monge R,Gomez L,Barber D,Lopez-Otin C,Armentia A,Salcedo G.Wheat and barley allergens associated with baker's asthma.Glycosylated subunits of the alpha-amylase-inhibitor family have enhanced IgE-binding capacity.Biochem.J.,1992,281:401-405
303.Sanchez-Monge R,Gomez L,Garcia-Olmedo F,Salcedo G.New dimeric inhibitor of heterologous alpha-amylase encoded by a duplicated gene in the short arm of chromosome 3B of wheat(Triticum aestivum L.).European Journal of Biochemistry,1989,183:37-40
304.Sander I,Merget R,Degens PO,Goldscheid N,Bruning T,Raulf-Heimsoth M. Comparison of wheat and rye flour skin prick test solutions for diagnosis of baker's asthma. Allergy, 2004, 59 (1) : 95-98
305.Sander I, Merget R, Leonhardt L, Chen Z, Raulf-Heimsoth M, Baur X. Prevalence of allergen-specific IgE in baker's asthma. Allergy, 1999, 54 (Suppl 52) : 50-51
306.Sandiford CP, Tatham AS, Fido R, Welch JA, Jones MG, Tee RD, et al. Identification of the major water/salt insoluble wheat proteins involved in cereal hypersensitivity. Clin. Exp. Allergy, 1997, 27: 1120-1129
307.Sano H, Ohashi Y. Involvement of small GTP-binding protein in defense signal-transduction pathways of higher plants. PNAS, 1995, 92: 4138-4144
308.Sasanuma T, Miyashita NT, Tsunewaki K. Wheat phylogeny determined by RFLP analysis of nuclear DNA. 3. Intra- and interspecific variations of five Aegilops Sitopsis species. Theor. Appl. Genet., 1996, 92: 928-934
309.Schroeder HE, Gollasch S, Moore A, Tabe LM, Craig S, Hardie DC, Chrispeels MJ, Spencer D, Higgins TJV. Bean [alpha]-Amylase Inhibitor Confers Resistance to the Pea Weevil (Bruchus pisorum) in Transgenic Peas (Pisum sativum L.) Plant Physiol, 1995,107: 1233-1239
310.Schroeder HW, Christensen JJ. Factors affecting resistance of wheat to scab caused by Gibberella zeae. Phytopathology, 1963, 53: 831-838
311.Schuler HT, Poppy MG, Kerry BR, Denholm L. Insect-resistant transgenic plants. Trends Biotechnol., 1998, 16: 168-174
312.Schwarz G, Sift A, Wenzel G, Mohler V. DHOLC scoring of a SNP between promoter sequences of HMW glutenin x-type alleles at the Glu-D1 locus in wheat. Journal of Agricultural and Food Chemistry, 2003, 51: 4263-4267
313.Schwarz PB. Impact of Fusarium head blight on malting and brewing quality of barley. In: Leonard, K.J. & Bushnell W.R. (eds.) Fusarium Head Blight of Wheat and Barley. St Paul: American Phytopathological Society Press, 2003, Pp. 395-419
314.Shen X, Kong L, Ohm H (2004) Fusarium head blight resistance in hexaploid wheat (Triticum aestivum) -Lophopyrum genetic lines and tagging of the alien chromatin by PCR markers. Theor. Appl. Genet., 108: 808-813
315.Shen X, Ohm H. Fusarium head blight resistance derived from Lophopyrum elongatum chromosome 7E and its augmentation with Fhb1 in wheat. Plant Breeding 2006,125: 424-429
316.Shen X, Ohm H. Molecular mapping of Thinopyrum-derived Fusarium head blight resistance in common wheat. Mol Breeding, 2007,20:131-140
317.Shewry PR, Halford NG, Belton PS, Tatham AS. The structure and properties of gluten: an elastic protein from wheat grain. Philosophical Transactions of the Royal Society of London: Biological Sciences, 2002c, 357: 133-142
318.Shewry PR, Halford NG, Tatham AS. High-molecular-weight subunits of wheat glutenin. J. Cereal Sci., 1992, 15: 105-120
319. She wry PR, Halford NG Cereal seed storage proteins: structures,properties and role in grain utilization. Journal of Experimental Botany, 2002a, 53: 947-958.
320.Shewry PR, Miflin BJ. Seed storage proteins of economically important cereals. Adv. Cereal Sci. Technol., 1985, 7:1 -84
321 .Shewry PR, Pandya MJ. in Seed Proteins (Shewry, P. R. and Casey, R., eds), 1999, pp. 563-586, Kluwer Academic Publishers, Dordrecht
322.Shewry PR, Tatham AS, Barro P, Lazzeri P. Biotechnology of bread making: unraveling and manipulating the multi-protein gluten complex. Bioltechnology, 1995b, 13:1185-1190
323.Shewry PR, Tatham AS. Disulphide Bonds in Wheat Gluten Proteins. Journal of Cereal Science, 1997,25: 207-227
324.Shewry PR. Beaudoin F. Jenkins J. Griffiths-Jones S. Mills NC. Plant protein families and their relationships to food allergy. Biochemical Society Transactions 2002b, 30: 906-910
325.Shewry PR. Napier JA. Tatham AS. Seed storage proteins: structures and biosynthesis. The Plant Cell, 1995a, 7: 945-956
326.Shewry PR. Plant storage proteins. Biol. Rev., 1995, 70: 375-426
327.Sicherev SH, DeSimone J, Furlong TJ. Peanut and tree nut allergic reactions in restaurants and food establishments. J. Allergy Clin. Immunol., 2001,108: 867-870
328.Silano V, Poerio E, Buonocore V. A model for the interaction of wheat monomeric and dimeric protein inhibitors with α-amylase. Molecular and Cellular Biochemistry, 1977, 18:87-91
329.Silva MC, Da M, Grossi de sa MF, Chrispeels MJ, Togawa RC, Neshich G. Analysis of structural and physico-chemical parameters involved in the specificity of binding between α-amylases and their inhibitors. Protein Engineering, 2000, 13:167-177
330.Simeone M, Gedye K, Mason-Gamer R, Gill B, Morris C. Conserved regulatory elements identified from a comparative puroindoline gene sequence survey of Triticum and Aegilops diploid taxa. J. Cereal Sci., 2006, 44: 21-33
331.Singh J, Appels R, Sharp PJ, Sherritt JH: Albumin polymorphism and mapping of a dimeric α-amylase inhibitor in wheat. Aust. J. Agric. Res., 2001b, 52: 1173-1179
332.Singh J, Blundell M, Tanner G, Skerritt JH. Albumin and globulin proteins of wheat flour: immunological and N-terminal sequence characterization. Journal of Cereal Science, 2001a, 34:85-103
333.Singh J, Skerritt JH. Chromosomal control of albumins and globulins in wheat grain assessed using different fractionation procedures. Journal of Cereal Science, 2001, 33: 163-181
334.Singh NK, Shepherd KW. Solubility behavior, synthesis, degradation and subcellular location of a new class of disulphide-linked proteins in wheat endosperm. Australian Journal of Plant Physiology, 1987, 14: 245-252
335.Smith-Huerta NL, Huerta AJ, Barnhart D, Waines JG. Genetic variation in wild diploid wheats Triticum monococcum var. boeoticum and T. urartu (Poaceae) . Theor. Appl. Genet, 1989, 78: 260-264
336.Solemimani VD, Baum BR, Johnson DA. Efficient validation of single nucleotide polymorphisms in plants by Allele-Specific PCR, with an example from Barley, Plant Molecular Biology Reporter, 2003, 21: 281-288
337.Somers DJ, Kirkpatrick R, Moniwa M, Walsh A. Mining single-nucleotide polymorphisms from hexaploid wheat ESTs. Genome, 2003,46: 431-437
338.Sommer SS, Groszbar AR, Bottema CDK. PCR amplification of specific alleles (PASA) is a general method for rapidly detecting known single base-pair changes. Biotechniques, 1992, 12: 82-87
339.Spena A, Viotti A, Pirrota V. A homologous repetitive block structure underlies the heterogeneity of heavy and light chain zein genes. EMBO J., 1982, 1: 1589-1594
340. StatSoft, Inc. Electronic Statistics Textbook. Tulsa, OK: StatSoft. [http://www.statsoft.com/textbook/stathome.html] 2006.
341.Steffenson BJ. Fusarium head blight of barley: impact, epidemics, management, and strategies for identifying and utilizing genetic resistance. In: Leonard, K.J. & Bushnell W.R. (eds.) Fusarium Head Blight of Wheat and Barley. St Paul: American Phytopathological Society Press, 2003. Pp. 241-295
342.Stoessl A, Unwin CH. The antifungal factors in barley. V. Antifungal activity of the hordatines. Can. J. Bot., 1978,48: 465-470
343.Stone PJ, Nicolas ME. Varietals differences in mature protein composition of wheat resulted from different rates of polymeraccumulation during grain filling, Aust. J. Plant Physiol., 1996,23: 727-737
344.Strobl S, Muhlhahn P, Bernstein R, Wiltscheck R, Maskos K, Wunderlich M, Huber R, Glockshuber R, Holak TA. Determination of the three-dimensional structure of the bifunctional alpha-amylase/trypsin inhibitor from ragi seeds by NMR spectroscopy. Biochemistry, 1995, 34: 8281-8293
345.Struck C, Mueller E, Martin H, Lohaus G The Uromyces fabae UfAAT3 gene encodes a general amino acid permease that prefers uptake of in planta scarce amino acids. Molecular Plant Pathology, 2004, 5: 183-189
346.Svensson B. Fukuda K. Nielsen PK. B0nsager.BC. Proteinaceous a-amylase inhibitors. BBA, 2004, 1696:145-156
347.Swofford DL. PAUP~*. Phylogenetic Analysis Using Parsimony ( ~*and Other Methods) . Version 4. Sinauer Associates, Sunderland. Massachusetts. 1998
348.Tai SSK, Wu LSH, Chen ECF, Tzen JTC. Molecular cloning of 11S globulin and 2S albumin, the two major seed storage proteins in sesame. J. Agric. Food Chem., 1999, 47:4932-4938
349.Takase K. Site-directed mutagenesis reveals critical importance of the catalytic site in binding of a-amylase by wheat proteinaceous inhibitor. Biochemistry, 1994, 33: 7925-7930
350.Talbert LE, Blake TK, Storlie EW, Levin M. Variability in wheat based on low-copy DNA sequence comparisons, Genome, 1995,38: 951-957
351.Tao HP, Adalsteins AE, Kasarda DD. Intermolecular disulfide bonds link specific high-molecular-weight glutenin subunits in wheat endosperm. Biochimica Biophysica Acta, 1992,1159: 13-21
352.Tenaillon MI, Swakins MC, Long AD, Gaut RL, Doebley JF, Gaut BS. Patterns of DNA sequence polymorphism along chromosome 1 of maize (Zea mays ssp. mays L.) . Proc. Natl. Acad. Sci. U.S.A., 2001,98: 9161-9166.
353.Terras FRG, Schoofs HME, Thevissen K, Osborn RW, Vanderleyden J, Cammue BPA, Broekaert WF. Synergistic enhancement of the antifungal activity of wheat and barley thionins by radish and oilseed rape 2S albumins and by barley trypsin inhibitors. Plant Physiol., 1993, 103: 1311-1319
354.Thouraya M, Emmanuelle B. Proteomic analysis of the efect of heat stress on hexaploid wheat grain: Characterization of heat-responsive from non-prolamins fraction. Proteomics, 2004,4: 505-513
355.Tiffin P, Gaut BS. Molecular Evolution of the Wound-Induced Serine Protease Inhibitor wipl in Zea and Related Genera. Mol. Biol. Evol., 2001, 18: 2092-2101
356.Titarenko E, Chrispeels MJ. cDNA cloning, bio-chemical characterization and inhibition by plant inhibitors of the α-amylases of the Western corn rootworm, Diabrotica vir-gifera virgifera. Insect Biochem. Mol. Biol., 2000, 30: 979-990
357.Tuleen NA, Hart GE. Isolation and characterization of wheat-Elytrigia elongata chromosome 3E and 5E addition and substitution lines. Genome, 1988, 30: 519-524
358.Van Slageren MW. Wild wheats: a monograph of Aegilops L. and Amblyopyrum (Jaub. & Spach) Eig (Poaceae). Wageningen Agric Univ Press, Wageningen 1994
359.Vittozzi L, Morisi G, Silano V. Continuous automated assay of alphα-amylase inhibitors. J. Sci. Food Agric, 1976,27 (5) : 449-453
360.Walia H, Wilson C, Wahid A, Condamine P, Cui X, Close TJ. Expression analysis of barley (Hordeum vulgare L.) during salinity stress. Func. Integr. Genomics, 2006,6: 143-156
361.Walsh BJ, Howden ME. A method for the detection of IgE binding sequences of allergens based on a modification of epitope mapping. J. Immunol. Methods, 1989, 121:275-280
362.Walton JD. Deconstructing the cell wall. Plant Physiol., 1994, 104: 1113-1118
363.Wan Y, Wang D , Shewry PR, Halford NG. Isolation and characterization of five novel high molecular weight subunit of glutenin genes from Triticum timopheevi and Aegilops cylindrica. Theor Appl Genet, 2002,104: 828-839
364. Wan YF, Yen C, Yang JL. The diversity of head-scab resistance in Triticeae and their relation to ecological conditions. Euphytica, 1997, 97: 277-281
365.Wang DG, Fan JB, Siao CJ, Berno A, Young P, Sapolsky R, Ghandour G, Perkins N, Winchester E, Spencer J, Kruglyak L, Stein L, Topaloglou T, Hubble E, Robinson E, Mittmann M, Morris MS, Shen N, Kilburn D, Rioux J, Nusbaum C, Rozen S, Hudson TJ, Lander ES. Large-scale identification, mapping, and genotyping of single-nucleotide polymorphisms in the human genome. Science, 1998, 280: 1077-1082
366.Wang JR, Wei YM, Yan ZH, Nevo E, Baum BR, Zheng YL. Molecular characterization of dimeric a-amylase inhibitor genes in wheat and development of genome allele-specific primers for the genes located on chromosome 3BS and 3DS. Journal of Cereal Science 2006b 43:360-368
367.Wang JR, Wei YM, Yan ZH, Zheng YL. Detection of single nucleotide polymorphisms in the 24 kDa dimeric α-amylase inhibitors from cultivated wheat and its diploid putative progenitors. Biochim. Biophys. Acta 2005 1723: 309-320
368.Wang JR, Wei YM, Yan ZH, Zheng YL. Genetic mapping of the wheat dimeric α-amylase inhibitor multi-gene family using allele-specific primers based on intergenomic SNPs. Plant molecular biology report, 2006c, 4 (3) : 1-8
369.Wang JR, Wei YM, Yan ZH, Zheng YL. SNP analysis and haplotype identification of the genes encoding monomeric a-amylase inhibitors. Genetica 2008, (online)
370.Wang JR, Yan ZH, Wei YM, Zheng YL. A novel HMW glutenin subunit gene Ee1.5 from Elytrigia elongata (Host) Nevski. Journal of cereal Science, 2004, 40: 289-294
371.Wang JR, Yan ZH, Wei YM, Zheng YL. Characterization of High-Molecular-Weight Glutenin Subunits Genes from Elytrigia elongata (Host) Nevski. Plant Breeding 2006a 125: 89-95
372.Wang JR, Zhang L, Wei YM, Yan ZH, Baum BR, Nevo E, Zheng YL. Sequence polymorphisms and relationships of dimeric a-amylase inhibitor genes in the B genomes of Triticum and S genomes of Aegilops. Plant Science, 2007a, 173: 1-11
373 .Wang L, Zhang K, Zhang L. Perfect phylogenetic networks with recombination. J. Comp. Bio., 2001, 8: 69-78
374.Wang RR-C, von Bothmer R, Dvorak J, Fedak G, Linde-Laursen I, Muramatsu M. Genome symbols in the Triticeae (Poaceae), in Proc. 2nd Int. Triticeae Symp., edited by Wang RR-C, Jensen KB, Jaussi C, Utah State Univ., Logan 1996, pp. 29-34
375.Warchalewski JR, Gralik J, Winiecki Z, et al. The effect of wheat a-amylase inhibitors incorporated into wheat-based artificial diets on development of Sitophilus granarius L., Tribolium confusum Duv., and Ephestia kuehniella Zell. J. Appl. Ent., 2002, 126: 161-168
376.Weegels PL, Hamer RJ, Schofield JD. Functional properties of wheat glutenin. Cereal Sci., 1996,23:1-1
377.Weiss W, Huber G, Engel KH, Pethran A, Dunn MJ, Gooley AA, et al. Identification and characterization of wheat grain albumin/globulin allergens. Electrophoresis, 1997, 18:826-33
378.Wendel JF. Genome evolution in polyploids. Plant Molecular Biology, 2000, 42: 225-249
379.Whitaker JR. a-amylase inhibitors of higher plants and microorganisms. in: J.E. Kinsella, W.G. Soucie (Eds.) , Proc. Protein Co-prod. Symp., Am. Oil Chem. Soc, Champaign, IL, 1988, pp. 354-380
380.Wieser H, Antes S, Seilmeier W. Quantitative determination of gluten protein types in wheat flour by reversed-phase high performance liquid chromatography. Cereal Chem, 1998,75 (5) : 644-650
381.Wiesner H, Seilmeier W, Belitz HD. Vergleichende Untersuehungen uber partiele Aminosauresequenzen yon Prolaminen und Glutelinen versehriedener Getreidearten. Z. bensm Unters Forseh, 1980, 170: 17
382.Wisniewska H, Chelkowski J. Influence of exogenic salicylic acid on Fusarium seedling blight reduction in barley. Acta Physiologiae Plantarum, 1999, 21: 63-66
383.Worland AJ, Gale MD, Law CN. wheat genetics. Wheat breeding, 1987, 2(1): 129-171
384.Wrigley CW. Giant protein with flour power. Nature, 1996, 381: 738-739
385.Xiao SH, Zhang XY, Yan CS, Lin H Germplasm improvement for pre-harvest sprouting resistance in Chinese white-grained wheat: An overview of the current strategy. Euphytica, 2002,126: 35-38
386.Xie DX, Freys BF, James S, Nieto-Rostro M, Tuner JG. COI1: an Arabidopsis gene required for jasmonate-regulated defence and ferticility. Science, 1998, 280: 1091-1094.
387.Yan ZH, Wan YF, Liu KF, Zheng YL, Wang DW. Identification of a novel HMW glutenin subunit and comparison of its amino acid sequence with those of homologous subunits. Chinese Science Bulletin., 2002,47 (3) : 220-225
388.Yan ZH, Wei YM, Wang JR, L DC, Dai SF, Zheng YL. Characterization of two HMW glutenin subunit genes from Taenitherum Nevski. Genetica, 2006, 127: 267-276
389.Yeh FC, Yang RC, Boyle T, Ye ZH, Mao JX. POPGENE, the user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Center, University of Alberta, Canada. 1977
390.Zawistowska U, Bietz JA, Bushuk W. Characterization of low-molecular-weight protein with high aflqnity for flour lipid from twowheat classes. Cereal Chem, 1986, 63: 414-419
391.Zhang H, Reader SM, Liu X, Jia JZ, Gale MD, Devos KM. Comparative genetic analysis of the Aegilops longissima and Ae. sharonensis genomes with common wheat, Theor.Appl.Genet.,2001,103:518-525
392.Zhang LQ,L.Dengcai,Y.Zehong,L.Xiujin,Z.Youliang,Z.Yonghong,Rapid changes of microsatellite flanking sequence in the allopolyploidization of new synthesized hexaploid wheat.Science in China Series C-Life Sciences,2004,47:553-561
393.Zheng Z,Mosher SL,Fan B,Klessig DF,Chen Z.Functional analysis of Arabidopsis WRKY25 transcription factor in plant defense against Pseudomonas syringae.BMC Plant Biol.2007,7:2
394.冯英,薛庆中.抗虫基因工程及其安全性.遗传,2001,23:571-576
395.陆维忠,程顺和,王裕中主编.小麦赤霉病研究,科学出版社2001,序
396.毛伯韧,吴兆苏.小麦种子休眠特性的遗传及其机理研究.中国农业科学,1983,6:53-58
397.王爱丽,裴玉贺,张倩,晏月明,小麦非醇溶性蛋白(non.prolamins)的研究进展.首都师范大学学报(自然科学版),2006,27:68-74
398.肖世和,陈孝,徐慧君,林志珊,杜丽璞.大麦α-淀粉酶抑制基因对小麦α-淀粉酶的抑制作用研究.作物学报,1998,24:763-767
399.肖世和,戴大庆,蒋华仁.抗收获前发芽小麦品种的初步研究.四川农业大学学报,1986,4:219-224
400.杨学举,卢少源,张荣芝.小麦籽粒蛋白质组分与面包烘焙品质性状关系的研究.中国粮油学报,1999,14:1-5
401.曾雄生.论小麦在古代中国之扩张.中国饮食文化,2005,1:1
2. Aboul-Soud MAM, Yun B-W, Harrier LA, Loake GJ. Transformation of Fusarium oxysporum by particle bombardment and characterisation of the resulting transformants expressing a GFP transgene. Mycopathologia, 2004, 158: 475-482
3. Adams DJ, Causier BE, Mellor KJ, Keer V, Milling R, Dada J. Regulation of chitin sinthase and chitinase in fungi. In: Muzzzarelli, RAA (ed.), Chitin Enzymology. 1993, pp 15-26. European Chitin Society, Lyon, France; Ancona, Italy
4. Alagiri S, Singh TP. Stability and kinetics of a bifunctional amylase/trypsin inhibitor. Biochim. Biophys. Acta, 1993, 1203: 77-84.
5. Allaby RG, Banerjee M, Brown TA. Evolution of the high molecular weight glutenin loci of A, B, D, and G genomes of wheat. Genome, 1999,42: 296-307
6. Altabella T, Chrispeels MJ. Tobacco plants transformed with αAI gene express an inhibitor of insect a-amylase in their seeds. Plant Physiol, 1990, 93: 805-810
7. Amano M, Ogawa H, Kojima K, Kamidaira T, Suetsugu S, Yoshihama M, et al. Identification of the major allergens in wheat flour responsible for baker's asthma. Biochem J., 1998, 330: 1229-1234
8. Anderson OD, Greene FC. The characterization and comparative analysis of High-molecular-weight glutenin genes from genome A and B of a hexaploid bread wheat. Theor. Appl. Genet., 1989, 77 : 689-700
9. Aragoncillo C, Rodriguez L, Carbonero P, Garcia-Olmedo F. Chromosomal control of non-gliadin proteins from 70% ethanol extract of wheat endosperm, Theor. Appl. Genet., 1975,45:322-326
10. Armentia A, Sanchez-Monge R, Gomez L, Barber D, Salcedo G. In vivo allergenic activities of eleven purified members of a major allergen family from wheat and barley flour. Clin. Exp. Allergy, 1993, 23: 410-415
11. Arocho A, Chen BY, Ladanyi M, Pan QL. Validation of the 2 (-Delta Delta Ct) calculation as an alternate method of data analysis for quantitative PCR of BCR-ABL P210 transcripts. Diagnostic Molecular Pathology, 2006, 15: 56-61
12. Asins MJ, Carbonell EA. A comparative study on variability and phylogeny of Triticum species. Theor. Appl. Genet., 1986, 72: 551-558
13. Badaeva ED, Friebe B, Gill BS. Genome differentiation in Aegilops. 1. Distribution of highly repetitive DNA sequences on chromosomes of diploid species. Genome, 1996, 39: 293-306
14. Bai GH, Shaner G. Management of wheat and barley to Fusarium head blight. Annu. Rev. Phytopathol., 2004,42: 135-161
15. Bai GH, Shaner G. Scab of wheat: prospects for control. Plant Dis., 1994, 78: 760-765
16. Bai GH, Shaner G. Variation in Fusarium graminearum and cultivar resistance to wheat scab. Plant Dis., 1996,80: 975-979
17. Baker JE. Interaction of partially-purified amylases from larval anagasta kuehniella (lepidoptera: pyralidae) with amylase inhibitors from wheat, Comp. Biochem. PhysioL, 1989, 9311: 239-246
18. Ban T. Evaluation of resistance to Fusarium head blight in indigenous Japanese species of Agropyron (Elymus) . Euphytica, 1997, 97: 39-44.
19. Bandelt HJ, Forster P, Rohl A. Median-joining networks for inferring intraspecific phytogenies. Mol. Biol. Evol., 1999,16: 37-48
20. Barber D, Sanchez-Monge R, Mendez E, Lazaro A, Garcia-Olmedo F, Salcedo G. New and trypsin inhibitors among the CM-proteins of barley (Hordeum vulgare). Biochim. Biophys. Acta, 1986, 869: 115-118
21. Bartels D, Thompson RD. The characterization of cDNA clones coding for wheat storage proteins. Nucleic Acids Res., 1983,11: 2961-2977
22. Battershell VG, Henry RJ. High performance liquid chromatography of α-amylases from germinating wheat and complexes with the α-amylase inhibitor from barley. J. Cereal Sci., 1990, 12: 73-81
23. Baud F, Pebay-Peyroula E, Cohen-Addad C, Odani S, Lehmann MS. Crystal structure of hydrophobic protein from soybean: a member of a new cysteine-rich family. J. Mol. Biol., 1993,231:877-887
24. Baum BR, Bailey LG. The origin of the A genome donor of wheats (Triticum: Poaceae) : A perspective based on the sequence variation of the 5S DNA gene units. Genet. Resour. Crop Evol., 2004, 51: 183-196
25. Baur X, Posch A. Characterized allergens causing bakers' asthma. Allergy, 1998, 53: 562-566
26. Baur X, Weiss W, Sauer W et al. Baking agents as a contributory cause of baker's asthma. Dtsch Med Wochenschr, 1988,113: 1275-1278
27. Baur X. Baker's asthma: causes and prevention. Int Arch Occup. Environ Health., 1999, 72: 292-296
28. Bean SR, Tilley M. Sepration of water-soluble proteins from cereals by high-performance capillary electrophoresis (HPCE) . Cereal Chem., 2003, 80: 505- 510
29. Bechtel DB, Kaleikau LA, Gaines RL, Seitz LM. The effects of Fusarium graminearum infection on wheat kernels. Cereal Chem., 1985, 62: 191-197
30. Bedetti C, Bozzini A, Silano V, Luciano V. Amylase protein inhibitors and the role of Aegilops species in polyploid wheat speciation. Biochim. Biophys. Acta, 1974, 362 (2) : 299-307
31. Belton PS. On the elasticity of wheat gluten. J. Cereal Sci, 1999, 29: 103-107
32. Bernardo A, Bai G, Guo P, Xiao K, Guenzi AC, Ayoubi P. Fusarium graminearum-induced changes in gene expression between Fusarium head blight-resistant and susceptible wheat cultivars. Funct Integr Genomics, 2007, 7: 67-77
33. Bishop JG, Dean AM, Mitchell-Olds T. Rapid evolution in plant chitinases: molecular targets of selection in plant-pathogen co-evolution. Proc. Natl Acad. Sci. USA. 2000, 97: 5322-5327
34. Blake NK, Lehfeldt BR, Hemphill A, Shan X, Talbert LE. DNA sequence analysis suggests a monophyletic origin of the wheat B genome. Proc. 9th Wheat Genet. Symp. 1998,2:14-16
35. Blake NK, Lehfeldt BR, Lavin M, Talbert LE. Phylogenetic reconstruction based on low copy DNA sequence data in an allopolyploid: The B genome of wheat. Genome, 1999,42:351-360
36. Blanco A, Resta P, Simeone R, Parmar S, Shewry PR, Sabelli PW, Lafiandra D. Chromosomal location of seed storage protein genes in the genome of Daspyrum villosum (L.) Candargy. Theor. Appl. Genet, 1991, 82: 358-362
37. Blunenthal C, Gras PW, Bakes F, Barlow EWR, Wrigley CW. Possible role for the Glu-D1 locus with respect to tolerance to dough-quality change after heat stress. Cereal Chem, 1995, 72:135-136
38. Bock SA. Prospective appraisal of complaint of adverse reaction to foods in children during the first 3 yeras of life. Pediatrics, 1987, 79: 683-688
39. Boisen S, Djurtoft R. Trypsin inhibitor from rye endosperm: purification and properties. Cereal. Chem., 1981a, 58: 194-198
40. Boisen S, Djurtoft R. Trypsin inhibitor from wheat endosperm: purification and characterization. Cereal. Chem., 1981b, 58:460-463
41. Boutilier K, Hattori J, Baum BR, Miki BL. Evolution of 2S albumin seed storage protein genes in the Brassicaceae. Biochem. Syst. Evol., 1999,27: 223-234
42. Boutrot F, Guirao A, Alary R, Joudrier P, Gautier M. Wheat non-specific lipid transfer protein genes display a complex pattern of expression in developing seeds. Biochim. Biophys. Acta., 2005, 1730: 114-125
43. Brandolini A, Vaccino P, Boggini G, Ozkan H, Kilian B, Salamini F. Quantification of genetic relationships among A genomes of wheats. Genome, 2006,49: 297-305
44. Breiman A, Bogher M, Sternberg H, Graur D. Variability and uniformity of mitochondrial DNA in populations of putative diploid ancestors of common wheat. Theor. Appl. Genet., 1991, 82: 201-208
45. Breiteneder H, Ebner C. Molecular and biochemical classification of plant-derived food allergens. Journal of Allergy and Clinical Immunology, 2000,106:27-36
46. Bryant D, Moulton V. Neighbor-Net: An agglomerative method for the construction of phylogenetic networks. Mol. Biol. Evol., 2004, 21: 255-265
47. Bundock PC, Henry RJ. Single nucleotide polymorphism, haplotype diversity and recombination in the Isa gene of barley. Theor Appl Genet, 2004,109: 543-551
48. Buonocore V, Biasi MGD, Giardina P, Poreio E, Silano V. Purification and properties of an a-amylase tetrameric inhibitor from wheat kernel. Biochim. Biophys. Acta, 1985, 831:40-48
49. Buonocore V, Petrucci T, Silano V. Wheat protein inhibitors of α-amylase. Phytochemistry, 1977,16: 811-820
50. Burhenne K, Kristensen BK, Rasmussen SK. A New Class of N-Hydroxy-cinnamoyltransferases. J. Biological. Chemistry, 2003,278: 13919-13927
51. Bustos AD, Jouve N. Characterization and phylogenetic analysis of the genes coding for high molecular weight glutenin subunits in three diploid specied of Aegilops. International Journal of Plant Sciences, 2006,167: 359-366
52. Bustos AD, Rubio P, Jouve N. Molecular characterization of the inactive allele of the gene Glu-Al and the development of a set of AS-PCR markers for HMW glutenins of wheat. Theor Appl Genet, 2000,100: 1085-1094
53. Carbonero P, Garcila-Olmedo F. in Seed Proteins (Shewry PR & Casey R eds), 1999, pp. 617-633, Kluwer Academic Publishers, Dordrecht
54. Carrano L, Nitti G, Buonocore V, Caporale C, Poerio E. An effective purification procedure of amylase and trypsin inhibitors from wheat flour; isolation of a new water-soluble. Plant Scienc, 1989, 65: 25-31
55. Castagna R, Maga G, Pernzin M, Heun M, Salamini F. RFLP-based genetic relationship of Einkorn wheats. Theor. Appl. Genet., 1994, 88: 818-823
56. Chantret N, Salse J, Sabot F, Rahman S, Bellec A, Laubin B, Dubois I, Dossat C, Sourdille P, Joudrier P, Gautier M-F, Cattolico L, Beckert M, Aubourg S, Weissenbach J, Caboche M, Bernard M, Leroy P, Chalhoubb B. Molecular Basis of Evolutionary Events That Shaped the Hardness Locus in Diploid and Polyploid Wheat Species (Triticum and Aegilops) . Plant Cell, 2005, 17: 1033-1045
57. Chelkowski J, Cierniewska A, Wakuliriski W. Mycotoxins in cereal grain Part 14. Histochemical examination of fusarium-damaged wheat kernels. Nahrung, 1990, 34: 357-361
58. Chen P, Liu D, Sun W. New countermeasures of breeding for scab resistance. In: Duben HJ, Gilchrist, Reeves J and McNab A (eds) , Fusarium Head Scab: Global Status and Future Prospects, CIMMYT, Mexico DF, 1997, pp. 86-92.
59. Chen ZY, Brown LR, Lax AR, Guo BZ, Cleveland TE, Russin JS. Resistance to Aspergillus flavus in corn kernels is associated with a 14-kDa protein. Phytopathology, 1998,88:276-281
60. Cho S, Chen W, Muehlbauer FJ. Constitutive expression of the Flavanone 3-hydroxylase gene related to pathotype-specific ascochyta blight resistance in Cicer arietinum L. Physiological and Molecular Plant Pathology, 2005, 67: 100-107
61. Chrispeels MJ, Grossi-de-Sa MF, Higgins TJV. Genetic engineering with α-amylase inhibitors seeds resistant to bruchids. Seed Sci. Res., 1998, 8: 257-263
62. Christeller JT. Evolutionary mechanisms acting on proteinase inhibitor variability. FEBS Journal, 2005, 272: 5710-5722
63. Cipollini D, Enright S, Traw MB, Bergelson J. Salicylic acid inhibits jasmonic acid-induced resistance of Arabidopsis thaliana to Spodoptera exigua. Mol. Ecol., 2004,13: 1643-1653
64. Collins FS, Guyer MS. Chakravarti A Variations on a theme: Cataloging human DNA sequence variations. Science, 1997, 278: 1580-1581
65. Cooper DN, Krawczak M. The mutational spectrum of single base-pair substitutions causing human genetic disease: patterns and predictions. Hum. Genet., 1990, 85: 55-74
66. Cooper DN, Youssoufian H. The CpG dinucleotide and human genetic disease. Hum. Genet., 1988, 78: 151-155
67. Cronin JK, Bundock PC, Henry RJ, Nevo E. Adaptive climatic molecular evolution in wild barley at the Isa defense locus. Proc Natl Acad Sci U.S.A., 2007,104: 2773-2778
68. D'Egidio MG, Nardi S, Vallega V. Grain, Flour, and Dough Characteristics of Selected Strains of Diploid Wheat, Triticum monococcum L. Cereal Chemisty, 1993, 70: 298-303
69. Damude HG, Zhang H., Farrall L., Ripp KG, Tomb J-F, Hollerbach D, Yadav NS. Identification of bifunctional △12/ω3 fatty acid desaturases for improving the ratio of ω3 to ω6 fatty acids in microbes and plants. PNAS, 2006,109: 9446-9451
70. Daud HM, Gustafson JP. Molecular evidence for Triticum speltoides as a B-genome progenitor of wheat (Triticum aestivum) . Genome, 1996, 39: 543-548
71. David A. Nelsen JR. Gluten-Sensitive Enteropathy (Celiac Disease) : More Common Than You Think. American Family Physican 2002, 66: 2259-2266
72. Davis JM, Wu H, Cooke JEK, Reed JM, Luce KS, Michler CH. Pathogen Challenge, Salicylic Acid, and Jasmonic Acid Regulate Expression of Chitinase Gene Homologs in Pine. MPMI, 2002b, 15: 380-387
73. Davis P. Mills C. Shewry P. Plant Food Allergens. Biochemical Society Transactions, 2002a, 30: 901-906
74. De Bustos A , Rubio P , Jouve N. Characterization of two gene subunits on the 1R chromosome of rye as orthologs of each of the Glu-1 genes of hexaploid wheat. Theor. Appl. Genet, 2001, 76 .513-529
75. De Bustos A, Jouve N. Characterization and analysis of new HMW-glutenin alleles encoded by the Glu-R1 locus of Secale cereale. Theor. Appl. Genet., 2003, 107: 74-83
76. De Leo F, Volpicella M, Licciulli F, Liuni S, Gallerani R, Ceci LR. PLANT-PIs: a database for plant protease inhibitors and their genes, Nucleic Acids Res., 2002, 30: 347-348
77. Dempsey DA, Shah J, Klessig DF. Salicylic acid and disease resistance in plants. Crit. Rev. Plant Sci., 1999,18: 547-575
78. Deponte R, Parlamenti R, Petrucci V, Silano V, Tomasi M. Albumin α-amylase inhibitor families from wheat flour. Cereal Chem., 1976, 53: 805-820
79. Derera NF, Bhatt GM, Mcmaster GJ. On the problem of preharvest sprouting of wheat. Euphytica, 1977,26: 299-308
80. Desmyter A, Spinelli S, Payan F, Lauwereys M, Wyns L, Muyldermans S, Cambillau C. Three camelid VHH domains in complex with porcine pancreatic α-amylase. J. Biol. Chem., 2002, 277: 23645-23650
81. Dilbirligi M, Erayman M, Campbell BT, Randhawa HS, Baenziger PS, Dweikat I, Gill KS. High-density mapping and comparative analysis of agronomically important traits on wheat chromosome 3A, Genomics, 2006, 88: 74-87
82. Doares SH, Narvaez-Vasquez J, Conconi A, Ryan CA. Salicylic acid inhibits synthesis of proteinase inhibitors in tomato leaves induced by systemin and jasmonic acid. Plant. Physiol, 1995, 108: 1741-1745
83. Dong Y-C ,Zheng D-S. Genetic resource of Chinese wheat. Pekin: China agricultural publishing company, 1998
84. Douliez JP, Michon T, Elmorjani K, Marion D. Structure, biology and technological functions of lipid transfer proteins and indolines, the major lipid binding proteins from cereal kernels. J. Cereal Sci., 2000, 32: 1-20
85. D'ovidio R, Masci S, Porcedu E. Development of a set of oligonucletide primers specific for genes at the Glu-1 complex loci of wheat. Theor. Appl. Genet., 1995, 91: 189-194
86. D'ovidio R, Porceddu E, Lafiandra D, et al. PCR analysis of gene encoding allelic variants of high molecular weight glutenin subunits at the Glu-Dl locus. Theor. Appl. Genet, 1994,88:175-180
87. Dowd C, Wilson IW, McFadden H. Gene Expression Profile Changes in Cotton Root and Hypocotyl Tissues in Response to Infection with Fusarium oxysporum f. sp. Vasinfectum. MPMI, 2004, 17: 654-667
88. Dubcovsky J, Luo MC, Dvorak J. Differentiation between homoeologous chromosomes 1A of wheat and 1Am of Triticum monococcum and its recognition by the wheat Ph1 locus. Proc. Natl. Acad. Sci. U.S.A., 1995, 92: 6645-6649
89. Dvorak J, Kasarda D D, Dietler M D, et al. Chromosomal location of seed storage protein genes in the genome of Elytrugia elongata. Can.J.Genet.Cytol, 1986, 28: 818-830
90. Dvorak J, Knott DR. Disomic and ditelosomic additions of diploid Agropyron elongatum chromosomes to triticum aestivum. Can. J. Genet. Cytol., 1974, 16: 399-417
91. Dvorak J, Tetizi P, Zhang HB, Resta P. The evolution of polyploid wheats: Identification of the A genome donor species. Genome, 1993, 36: 21-31
92. Dvorak J. Homology between Agropyron elongatum chromosomes and triticum aestivum chromosomes. Can. J. Genet. Cytol., 1980,22: 237-259
93. El-Araby MM, Ahmed HFS. Differential effect of jasmonic acid on the defense of faba bean against Fusarium wilt: changes in protein and DNA patterns, peroxidase and esterase isozymes. International Journal of Agriculture and Biology, 2004, 6: 226-232
94. Engelberth J, Koch T, Schuler G, Bachmann N, Rechtenbach J, Boland W. Ion channel-forming alamethicin is a potent elicitor of volatile biosynthesis and tendril coiling. Cross talk between jasmonate and salicylate signaling in lima bean. Plant Physiol, 2001, 125: 369-377
95. Epple P, Apel K, Bohlmann H. Overexpression of an endogenous thionin enhances resistance of Arabidopsis against Fusarium oxysporum. Plant Cell, 1997, 9: 509-520
96. Erayman M, Sandhu D, Sidhu D, Dilbirligi M, Baenziger PS, Gill KS. Demarcating the gene-rich regions of the wheat genome. Nucleic. Acids Res., 32: 2004, 3546-3565
97. Erlich PR, Raven PH. Butterflies and plants: a study in co-evolution. Evolution, 1964, 1:18
98. Fahima T, Roder MS, Wendehake VM, Nevo E. Microsatellite polymorphism in antural populations of wild emmer wheat, Triticum dicocoides, in Israel. Theor. Appl. Genet, 2002,104:17-29
99. Fahima T, Sun GL, Beharav A, Krugman T, Beiles A, Nevo E. RAPD polymorphism of wild emmer wheat populations, Triticum dicocoides, in Israel. Theor. Appl. Genet., 1999,98:434-447
100.Faris J, Sirikhachornkit A, Haselkorn R, Gill B, Gornicki P. Chromosome Mapping and Phylogenetic Analysis of the Cytosolic Acetyl-CoA Carboxylase Loci in Wheat. Mol. Bio. Evol, 2001, 18: 1720-1733
101.Fedak G, Cao W, Han F, Savard M, Gilbert J, Xue A. Germplasm enhancement for FHB resistance in spring wheat through alien introgression. In: S.M. Canty, T. Boring, K. Versdahl, J. Wardwell & R.W. Ward (Eds.), Proc. 2nd Int Symp on Fusarium Head Blight, Orlando, Michigan State University, East Lansing, MI, 2004, pp. 56-57.
102.Fedak G Sources of resistance to Fusarium head blight. In: W.J. Raupp, Z. Ma, P.D. Chen & D.J. Liu (Eds.) , Proc Int Symp Wheat Improv Scab Resist, Suzhou and Nanjing, China, 2000, pp. 4.
103.Feldman M. Origin of cultivated wheat, in: A.P. Bonjean, W.J. Angus (eds.) . The world Wheat Book: A History of Wheat Breeding, Intercept Ltd, London, Paris, New York, 2000, pp. 3-56
104.Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution, 1985,39:783-791
105.Felsenstein J. PHYLIP. (Phylogeny Inference Package) version 3.6. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle. Version 3.61. 2004, available at http://evolution.gs.washington.edu/phylip.html
106.Felton GW. Nutritive quality of plant protein: sources of variation and insect herbivore response. Arch Insect Biochem Physiol, 1996, 32: 107-130.
107.Feng GH, Richardson M, Chen MS, Kramer KJ, Morgan TD, Reeck GR. α-Amylase inhibitors from wheat: a sequences and patterns of inhibition of insect and human α-amylases. Insect Biochem. Mol. Bio., 1996,26: 419-426
108.Finnie C, Melchior S, Roepstorff P, Svensson B. Proteome analysis of grain filling and seed maturation in barley. Plant Physiol., 2002, 129: 1308-1319
109.Fischer WN, Andre B, Rentsch D, Krolkiewicz S, Tegeder M, Breitkreuz K, Frommer WB. Amino acid transport in plants. Trend in Plant Science, 1998, 3: 188-195
110.Flavell RB, Goldsborough AP. Robert LS. Schnick D. Thompson RD. Genetic variation in wheat HMW glutenin subunits and the molecular basis of bread-making quality. Biotechnology, 1989, 7: 1281-1285
111.Franco OL, Ridgen DJ, Melo FR, Bloch C Jr., Silva CP, Grossi de Sa MF. Activity of wheat α-amylase inhibitors toward bruchid a-amylases and structural explanation of observed specificities. Eur. J. Biochem., 2000, 267: 2166-2173
112.Franco OL, Rigden DJ, Melo FR, Grossi-de-Sa MF. Plant a-amylase inhibitors and their interaction with insect α-amylases Structure, function and potential for crop protection. Eur. J. Biochem., 2002, 269: 397-412
113.Franken J, Stephan U, Meyer HE, Konig W. Identification of alpha-amylase inhibitor as a major allergen of wheat flour. Int. Arch. Allergy Immunol., 1994, 104: 171-174
114.Friebe B, Tuleen N, Jiang J, Gill BS. Standard karyotype of Triticum longissimum and its cytogenetic relationship with T. aestivum. Genome, 1993, 36: 731-742
115.Gale MD, Law CN, Chojecki AJ. Genetic control of a-amylase production in wheat. Theor. Appl. Genet., 1983, 64: 309-316
116.Galili S, Avivi Y, Millet E, Feldman M. RFLP based analysis of three RbcS subfamilies in diploid and polyploid species of wheat. Mol. Gen. Genet., 2000, 263: 674-680
117.Garbay B, Tautu MT, Costaglioli P. Low level of pathogenesis-related protein 1 mRNA expression in 15-day-old Arabidopsis cer6 and cer2 eceriferum mutants. Plant Science, 2007,172:299-305
118.Garcia-Casado G, Armentia A, Sanchez-Monge R, Malpica JM, Salcedo G. Rye flour allergens associated with baker's asthma. Correlation between in vivo and in vitro activities and comparison with their wheat and barley homologues. Clinical and Experimental Allergy., 1996,26: 428-435
119.Garcia-Casado G, Sanchez-Monge R, L6pez-Otin C, Salcedo G. Rye chromosome arm 3RS encodes a homodimeric inhibitor of insect α-amylase. Theor. Appl. Genet., 1994a, 89: 60-63
120.Garcia-Casado G, Sanchez-Monge R, Lopez-Otin C, Salcedo G Rye Inhibitors of Animal α-amylases Show Different Specifities, Aggregative Properties and IgE-binding Capacities than Their Homologues from Wheat and Barley European Journal of Biochemistry, 1994b, 224 (2) :525-530
121.Garcia-Maroto F, Cabonero P, Garcia-Olmedo F. Site-directed mutagenesis and expression in Escherichia coli of WMAI-1, a wheat monomeric inhibitor of insect α-amylase. Plant Mol. Biol., 1991,17:1005-1011
122.Garcia-Olmedo F, Salcedo G, Sanchez-Monge R, Gomez L, Royo J, Carbonero P. Plant proteinaceous inhibitors of proteinases and a-amylases. Oxford Surv. Plant Mol. Cell Biol., 1987,4:275-334
123.Garg K, Green P, Nickerson DA. Identification of candidate coding region single nucleotide polymorphisms in 165 human genes using assembled expressed sequence tags. Genome Research, 1999, 9: 1087-1092
124.Gatehouse AMR, Boulter D, Hilder VA. Potential of plant-derived genes in the genetic manipulation of crops for insect resistance. In Biotechnology in Agriculture, Plant Genetic Manipulation for Crop Protection. 1992, Vol. 7, pp. 155-181. CAB International, Wallingford, UK.
125.Gatehouse AMR, Gatehouse JA. Identifying proteins with insecticidal activity: use of encoding genes to produce insect-resistant transgenic crops. Pest Sci., 1998, 52: 165-175
126.Gianibelli MC, Solomon RG. A novel y-type high Mr glutenin subunit (12.4~t) present in Triticum tauschii. J. Cereal Sci., 2003, 37: 253-256.
127.Gilbert J, Tekauz A. Review: Recent developments in research on Fusarium head blight of wheat in Canada. Can. J. Plant Pathol., 2000,22: 1-8
128.Golkari S, Gilbert J, Parshar S, Procunier JD. Microarray analysis of Fusarium graminearum-induced wheat genes, identification of organ-specific and differentially expressed genes. Plant Biotechnology Journal, 2007, 5: 38-49
129.Gomez L, Sanchez-Monge R, Lopez-Otin C, et al. Wheat inhibitors of heterologous α-amylases. Plant Physiol., 1991, 96: 768-774
130.Gorinstein S, Zemser M, Fliess A, Shnitman I, Paredes-Lopez O, Yamamoto K, Kobayashi S, Taniguchi H. Computational analysis of the amino acid residue sequences of amaranth and some other proteins. Biosci. Biotechnol. Biochem., 1998, 62: 1845-1851
131.Gu YQ, Coleman-Derr D, Kong X, Anderson OD. Rapid genome evolution revealed by comparative sequence analysis of orthologous region from four Triticeae genomes. Plant Physiology, 2004a, 135: 459-470
132.Gu YQ, Crossman C, Kong X, Luo MC, You FM, Coleman-Derr D, Dubcovsky J, Anderson OD. Genomic organization of the complex α-gliadin gene loci in wheat, Theor. Appl. Genet., 2004b, 109: 648-657
133.Guo ZF, Yan ZH, Wang JR, Wei YM, Zheng YL. Characterization of HMW prolamines and their coding sequences from Crithopsis delileana. Hereditas, 2005, 142:56-64
134.Gupta RB, Khan K, MacRitchie F. Biochemical basis of flour properties in bread wheats. I . Effects of variation in the quantity and size distribution of polymeric protein. J. Cereal Sci., 1993,18: 23-41
135.Gupta RB, Popineau Y, Lefebvre J, Cornect M, Lawrence GJ, MacRitchie F. Biochemical basis of flour properties in bread wheats. Ⅱ. Changes in polymeric protein formation and dough/gluten properties associated with the loss of low Mr or high Mr glutenin subunits. J. Cereal Sci., 1995, 21: 103-116
136.Gupta RB, Shepherd KW, MacRitchie F. Genetic control and biochemical properties of some high molecular weight albumins in bread wheat. Journal of Cereal Science, 1991,13:221-235
137.Gupta RB, Sphepherd KW. Production of multiple wheat-rye 1RS translocation stocks and genetic analysis of LMW subunits of glutenin and gliadins in wheat using these stocks. Theor. Appl. Genet., 1993, 85:719-728
138.Halford NG, Forde J, Anderson OD, Greene FC, Shewry PR. The nucleotide and deduced amino-acid sequences of an HMWglutenin subunit gene from chromosome 1B of bread wheat (Triticum aestivum L.) and comparison with those of genes from chromosomes 1A and 1D. Theor. Appl. Genet, 1987,75:117-126
139.Hammer K, Filatenko AA, Korzun V. Microsatellite markers: A new tool for distinguishing diploid wheat species. Genet. Resour. Crop Evol., 2000,47: 497-505
140.Han FP, Fedak G Molecular characterization of partial amphiploids from Triticum durum x tetraploid Thinopyrum elongatum as novel source of resistance to wheat Fusarium head blight. In: Proceeding of the 10th International Wheat Genetics Symposium, 2003, Paestum Italy. Edited by Pogna NE et al. pp 1148-1150
141.Hanson DG, Vaz N, Maia L, et al. Inhibition of specific immune response by feeding protein antigen. Int. Arch Allergy Appl. Immunol, 1977, 55: 1518-1524
142.Harding RM, Fullerton SM, Griffiths RC, Bond J, Cox MJ, Schneider JA, Moulin DS, Clegg JB. Archaic African and Asian lineages in the genetic ancestry of modern humans. Am. J. Hum. Genet., 1997, 60: 772-789
143.Harlan JR, Zohary D. Distribution of wild wheat and barley. Science, 1966, 153: 1074-1080
144.Hart LP, Pestka JJ, Liu MF. Efect of kenel development and period on production of deoxynivalenol in wheat infected with Gibberella zeae. Phytopathology, 1984, 74: 1415-1418
145.Hejgaard J, Bjorn SE, Nielson G Rye chromosomes carrying structure genes for the major grain protease inhibitor. Hereditas, 1984, 101: 257-259
146.Hilder V, Gatehouse A, Sheerman S, Barker R, Boulter D. A novelmechanismof insect resistance engineered into tobacco. Nature, 1987, 330: 160-163
147.Hillis DM, Bull JJ. An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis. Systematic Biology, 1993,42: 182-192
148.Himer A, Ladwig F, Stransky H, Okumoto S, Keinath M, Harms A, Frommer WB, Koch W. Arabidopsis LHT1 Is a High-Affinity Transporter for Cellular Amino Acid Uptake in Both Root Epidermis and Leaf Mesophyll. Plant Cell, 2006, 18: 1931-1946
149.Hoffmann L, Maury S, Martz F, Geofrroy P, Legrand M. Purification, cloning, and properties of an acyltransferase controlling shikimate and quinate ester intermediates in phenylpropanoid metabolism. J. Biol. Chem., 2003,278: 95-103
150.Holliday R, Girgg WG DNA methylation mutation. Mutation Res., 1993,285: 61-67
151.Holliday RA. Mechanism for gene conversion in fungi. Genet. Res., 1964, 5: 282-304
152.Houba R, Doekes G, Heederik D. Occupational respiratory allergy in bakery workers: a review of the literature. Am. J. Respir. Crit. Care. Med., 1998, 158: 1499-1503
153.Hsiao C, Chatterton NJ, Asay KH. Phylogenetic relationships of the monogenomic species of the wheat tribe, Triticeae (Poaceae) , inferred from nuclear rDNA (internal transcribed spacer) sequences. Genome, 1995, 38: 211-231
154.Huang S, Sirikhachornkit A, Fans JD, Su X, Gill BS, Haselkorn R, Gornicki P. Phylogenetic analysis of the acetyl-CoA carboxylase and 3-phosphoglycerate kinase loci in wheat and other grasses. Plant Mol. Biol., 2002b, 48: 805-820
155.Huang S, Sirikhachornkit A, Su X, Faris J, Gill B, Haselkorn R, Gornicki P. Genes encoding plastid acetyl-CoA carboxylase and 3-phosphoglycerate kinase of the Triticum/Aegilops complex and the evolutionary history of polyploid wheat, Proc. Natl. Acad. Sci. USA. 2002a, 99: 8133-8138
156.Huebner FR, Bietz JA. Improvements in wheat protein analysis and quality prediction by reversed phase high performance liquid chromatography. cereal chemistry, 1987. 64: 15-20
157.Huelsenbeck JP, Ronquist F. MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics, 2001,17: 754-755
158.Hughes DA, Mills C. Food allergy: a problem on the increase. Biologist (London) , 2001,48:201-204
159.Husby S, Mestecky J, Moldozeanu Z, et al. Oral tolerance in humans T cell but not B cell tolerance after antigen feeding. J. Immunol., 1994, 152: 4463-4470
160.Huson DH, Bryant D. User manual for SplitsTree 4. 2005, available at http ://www. splits tree. org
161.Irizarry RA, Hobbs B, Collin F, Beazer-Barclay YD, Antonellis KJ, Scherf U. Speed TP. Exploration, normalization and summaries of high density oligonucleotide array probe level data. Biostatistics, 2002, 4: 249-264
162.Iulek J, Franco OL, Silva M, Slivinski CT, Bloch C Jr., Rigden DJ, Grossi de Sa MR Purification, biochemical characterization and partial primary structure of a new α-amylase inhibitor from Secale cereale (rye) . Int. J. Biochem. Cell Biol., 2000, 32: 1195-1204
163.Jackowiak H, Packa D, Wiwart M, Perkowski J, Busko M, Borusiewicz A. Scanning electron microscopy of mature wheat kernels infected with Fusarium culmorum. J. Appl. Genet., 2002,43A: 167-176
164.Jarrett SJ, Marschke RJ, Symons MH, Gibson CE, Fox GP, Henry RJ. Alpha-amylase/subtilisin inhibitor levels in Australian barleys. J. Cereal Sci., 1997,25: 261-266
165.Jauhar PP, Peterson TS. Hybrids between durum wheat and Thinopyrum junceiforme: Prospects for breeding for scab resistance. Euphytica, 2001, 118: 127-136
166.Jiang J, Gill BS. Different species-specific chromosome translocations in Triticum timopheevi and Triticum turgidum support the diphyletic origin of polyploid wheats. Chromo Research, 1994,2: 59-64
167.Jiang QT, Wei YM, Wang JR, Yan ZH, Zheng YL. Isolation and Sequence Analysis of HMW Glutenin Subunit 1Dy10.1 Ecoding Gene from Xinjiang Wheat (Triticum petropavlovskyi Udacz. et Migusch). Agricultral Science in China, 2006, 5(2): 81-89
168.Jobb G. TreeFinder Version of October 2005. Munich, Germany. Distributed by the author at http://www.treefinder.de
169.Johnson BL, Dhaliwal HS. Reproduction isolation of Triticum boeoticum and Triticum urartu and the origin of the tetraploid wheats. Am. J. Bot., 1976, 63: 1088-1094
170.Jouanin L, Bonade-Bottino M, Girard C, Morrot G, Giband M. Transgenic plants for insect resistance. Plant Sci., 1998,131:1-11
171.Kang Z, Buchenauer H. Ultrastructural and immunocytochemical investigation of pathogen development and host responses in resistant and susceptible wheat spikes infected by Fusarium culmorum. Physiol. Mol. Plant Pathol., 2000b, 57: 255-268
172.Kashkush K, Feldman M, Levy AA. Gene loss, silencing and activation in a newly synthesized wheat allotetraploid. Genetics, 2002,160: 1651-1659
173.Kashkush K, Feldman M, Levy AA. Transcriptional activation of retrotransposons alters the expression of adjacent genes in wheat. Nature Genetics, 2003, 33: 102-106
174.Keck B, Kohler P, Wieser H. Disulfate bonds in wheat gluten: cystine peptides derived from gluten proteins following peptic and thermolytic digestion. Z. Lebensm.Unters. Forsch 1995, 200: 432-439
175.Kihara H. Wheat studies-restrospect and prospects. Crop Science, 1982, 40: 1742-1749
176.Kohler P, Belitz H-D, Wieser H. Disulfate bonds in wheat gluten: further cysteine peptides from high molecular weight (HMW) and low molecular weight (LMW) subunits of glutenin and γ-gliadins. Z Lebensm Unters Forsch, 1993, 196: 239-247
177.Kolattukudy PE, Rogers LM, Li D, Hwang C-S, Flaishman MA. Surface signalling in pathogenesis. Proc. Natl. Acad. Sci. USA, 1995, 92: 4080-4087
178.Kolderup F. Effects of temperature, photoperiod and light quantity on protein production in wheat grains. J. Sci. Food Agric. 1975, 26: 583-592
179.Konarev AV. Interaction of insect digestive enzymes with plant protein inhibitors and host-parasite co-evolution. Euphytica, 1996, 92: 89-94
180.Kreis M, Shewry PR, Forde BG, Forde J, Miflin BJ. Structure and evolution of seed storage proteins and their genes with particular reference to those of wheat, barley and rye. Oxf Surv Plant Mol Cell Biol, 1985,2: 253-317
181.Kumar S, Tamura K, Nei M. MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform, 2004, 5: 150-163
182.Lankisch M, Layer P, Rizza RA. DiMagno EP. Acute Postprandial Gastrointestinal and Metabolic Effects of Wheat Amylase Inhibitor (WAI) in Normal, Obese, and Diabetic Humans. Pancreas, 1998, 17 (2) : 176-181
183.Larsson S. Stressful times for the plant stress-insect performance hypothesis. Oikos, 1989,56:277-283.
184.Lawrence GJ, Shepherd KW. Chromosomal location of genes controlling seed proteins in species related to wheat. Theor. Appl. Genet., 1981, 59: 25-31
185.LeCorre V, Bernard M. Assessment of the type and degree of restriction fragment length polymorphism (RFLP) in diploid species of the genus Triticum. Theor. Appl. Genet, 1995,90: 1063-1067
186.Lehtio J, Teeri TT, Nygren P-A. Alpha-amylase inhibitors selected from a combinatorial library of a cellulose binding domain scaffold. Proteins, 2000, 41: 316-322
187.Lerche MH, Poulsen FM. Solution structure of barley lipid transfer protein complexed with palmitate. Two different binding modes of palmitate in the homologous maize and barley nonspecific lipid transfer proteins. Protein Sci., 1998, 7: 2490-2498
188.Levy AA, Feldman M. The impact of polyploidy on grass genome evolution. Plant Physiology, 2002, 130: 1587-1593
189.Lew EJL, Kuzmicky DD, Kasarda DD. Characterization of low molecular weight glutenin subunits by reversed-phase high-performance liquid chromatography, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and N-terminal amino acid sequencing. Cereal Chem, 1992, 69: 508-515
190.Lindsay MP, Tamas L, Appels R, Skerritt JH. Direct evidence that the number and location of cysteine residues affect glutenin polymer structure. J. Cereal Sci., 2000, 31: 321-333
191.Liu B, Segal G, Rong JK, Feldman M. A chromosome-specific sequence common to the B genome of polyploid wheat and Aegilops searsii. Plant Syst. Evol., 2003b, 241: 55-66
192.Liu Z, Yan Z, Wan Y, Liu K , Zheng Y, Wang D. Analysis of HMW glutenin subunits and their coding sequences in two dipliod Aegilops species. Theor Appl Genet, 2003a, 106:1368-1378
193.Lorito HJM, Brederode FT, Hayes CK, Woo SL, Noviello C, Williamas DL, Harman GE. Proteinase inhibitors from plants as a novel class of fungicides. Mol. Plant-Microbe Interact., 1994, 7: 525-527 .
194.Louda SM, Collinge SK. Plant resistance to insect herbivores: a field test of the environmental stress hypothesis. Ecology 1992, 73:153-169.
195.Lowney E. Suppression of contact sensirization in man by prior feeding of antigen. J Invest Dermatol, 1973, 61: 90-93
196.Lu S, Deng P, Liu X, Luo J, Han R, Gu X, Liang S, Wang X, Li F, Lozanov V, Patthy A, Pongor S. Solution structure of the major α-amylase inhibitor of the crop plant amaranth. J. Biol. Chem., 1999,274: 20473-20478
197.Luo MC, Yang ZL, Kota RS, Dvorak J. Recombination of chromosomes 3Am and 5 Am of Triticum monococcum with homoeologous chromosomes 3A and 5A of wheat: The distribution of recombination across chromosomes. Genetics, 2000, 154: 1301-1308
198.Lyons A, Richardson M, Tatham AS, Shewry PR. Characterization of homologous inhibitors of trypsin and the a-amylase. Biochim. Biophys. Acta, 1987, 915: 305-313
199.MacGregor EA, Bazin SL, Ens EW, Lahnstein J, Macri LJ, Shirley NJ, MacGregor AW. Structural models of limit dextrinase inhibitors from barley. J. Cereal Sci., 2000, 31:79-90
200.MacGregor EA, Janecek S, Svensson B. Relationship of sequence and structure to specificity in the a-amylase family of enzymes. Biochim. Biophys. Acta, 2001, 1546: 1-20
201.Mackie AM, Lagudah ES, Sharp PJ. Molecular and biochemical characterization of HMW glutenin subunits from T. tauschii and the D genome of hexapoid wheat . J. Cereal Sci., 1996,23:213-225
202.Maddison WP, Maddison DR. Mesquite: a modular system for evolutionary analysis. Version 1.06, 2005, available at http://mesquiteproject.org
203.Maeda K, Kakabayashi S, Matubara H. Complete amino acid sequence of an α-amylase inhibitor in wheat kernel (0.19-inhibitor) . Biochim. Biophys. Acta, 1985, 828:213-221
204.Maeda K. The complete amino-acid sequence of the endogenous a-amylase inhibitor in wheat. Biochem. Biophys. Acta, 1983, 871: 250-256
205.Maestra B, Naranjo T. Homoeologous relationships of Aegilops speltoides chromosomes to bread wheat. Theor. Appl. Genet, 1998, 97: 181-186
206.Maestra B, Naranjo T. Homoeologous relationships of Triticum sharonense chromosomes to T. aestivum. Theor. Appl. Genet., 1997, 94: 657-663
207.Mandal S. Mandal RK. Seed storage proteins and approaches for improvement of their nutritional quality by genetic engineering. Current Science, 2000, 79: 576-589
208.Marth GT, Korf I, Yandell MD, Yeh RT, Gu Z, Zakeri H, Stiziel NO, Hillier L, Kwok PY, Gish WR. A general approach to single-nucleotide polymorphism discovery. Nature Genet., 1999, 23: 452-456
209.Maruyama N, Ichise K, Katsube T, Kishimoto T, Kawase S, Matsumura Y, et al. Identification of major wheat allergens by means of the Escherichia coli expression system. Eur. J. Biochem, 1998,255: 739-745
210.Masojc P, Zawitowski J, Howes NK. Polymorphim and chromosomal location of endogenousa-amylase inhibitor genes in common wheat. Theor Appl. Genet., 1993, 85: 1043-1048
211.Matsuo H, Kohno K, Niihara H, Morita E. Specific IgE Determination to Epitope Peptides of ω-Gliadin and High Molecular Weight Glutenin Subunit Is a Useful Tool for Diagnosis of Wheat-Dependent Exercise-Induced Anaphylaxis. The Journal of Immunology, 2005, 175: 8116-8122
212.Matsuo H, Morita E, Tatham AS, Morimoto K, Horikawa T, Osuna H, Ikezawa Z, Kaneko S, Kohno K, Dekio S. Identification of the IgE-binding epitope in γ-5 gliadin, a major allergen in wheat-dependent exerciseinduced anaphylaxis. J. Biol. Chem., 2004,279: 12135-12140
213.Mattson WJ, Haack RA. The role of drought in outbreaks of plant-eating insects. BioScience, 1987, 37: 110-118
214.McEvoy PB. Insect-plant interactions on a planet of weeds. Entomologia Experimentalis et Applicata, 2002, 104: 165-179
215.McMullen M, Jones R, Gallenberg D. Scab of wheat and barley: A re-emerging disease of devastating impact. Plant Dis., 1997, 81: 1340-1348
216.Mena M, Sanchez-Monge R, Gomez L, Salcedo G. Carbonero P. Arnajor barley allergen associated with baker's asthma disease is a glycosylated monomeric inhibitor of insect alpha-amylase: cDNA cloning and chromosomal location of the gene. Plant Mol.Biol., 1992,20:451-458
217.Mentewab A, Rezanoor HN, Gosman N, Worland AJ, Nicholson P. Chromosomal location of Fusarium head blight resistance genes and analysis of the relationship between resistance to head blight and brown foot rot. Plant Breed., 2000, 119: 15-20
218.Mesterhazy A. Types and components of resistance to Fusarium head blight of wheat. Plant Breed, 1995, 114: 337-386
219.Metakovsky EV, Baboev SK. Polymorphism of gliadin and unusual gliadin alleles in Triticum boeoticum. Genome, 1992,35: 1007-1012
220.Miedaner T. Review. Breeding wheat and rye for resistance to Fusarium diseases. Plant Breed., 1997, 116: 201-220
221.Miller T E. Systematicas and evolution. In: Wheat Breeding-its scientific basis. Edited by Lupton F G, Chapman and Hall Ltd. 1987, 1-28
222.Millerd A. Biochemistry of legume seed proteins. Ann. Rev. Plant Physiol., 1975, 26: 53-72
223.Miyashita NT, Mori N, Tsunewaki K. Molecular variation in chloroplast DNA regions in ancestral species of wheat. Genetics, 1994,137: 883-889
224.Moghaddam M, Ehdaie B, Waines JG. Genetic diversity in populations of wild wheat Triticum urartu Tum.ex.Gandil. revealed by isozyme markers. Genetic Resources and Crop Evolution, 2000,47: 323-334
225.Molodchenkova OO, Adamovskaya VG, Levitskii YA, Gontarenko OV, Sokolov VM. Maize Response to Salicylic Acid and Fusarium moniliforme. Applied Biochemistry and Microbiology, 2002, 38: 381-385
226.Montebove L, De Pace C, Jan CC, Scarascia Mugnozza GT, Qualset CO. Chromosomal location of isozyme and seed storage protein genes in Dasypyrum villosum (L.) Candargy. Theor. Appl. Genet, 1987, 73: 836-845
227.Morita E, Matsuo H, Mihara S, Morimoto K, Savage AW, Tatham AS. Fast γ-gliadin is a-major allergen in wheat-dependent exercise-induced anaphylaxis. J. Dermatol. Sci, 2003, 33:99-104
228.Morris LD, Raupp WJ, Gill BS. Isolation of Ht genome chromosome additions from polyploid Elymus trachycaulus (StStHtHt) into common wheat (Triticum aestivum) . Genome, 1990,33: 16-22
229.Mundy J, Hejgaard J, Svendsen I. Characterization of a bifunctional wheat inhibitor of endogenous α-amylase and subtilisin. FEBS Lett, 1984, 167: 210-214
230.Mundy J, Svendsen I, Heigaard J. Barley α-amylase/subtilisin inhibitor. I . Isolation and characterization. Carlsberg Res. Commun., 1983,48: 81-90
231.Murray M, Thompson WF. Rapid isolation of high molecular weight plant DNA. Nucleic. Acids Res., 1980, 8: 4321-4325
232.Mustalahti K, Lohiniemi S, Collin P, Vuolteenaho N, Laippala P. Gluten-Free Diet and Quality of Life in Patients with Screen-Detected Celiac Disease. Effective Clinical Practice, 2002, 5: 105-113
233.Nagasue A, Fukamachi H, Ikenaga H, Funatsu G. The amino acid sequence of barley rootlet trypsin inhibitor. Agric. Biol. Chem., 1988, 52: 1505-1514
234.Naranjo T. Chromosome structure of Triticum longissimum relative to wh(?)at. Theor. Appl. Genet., 1995,91: 105-109
235.Nawrath C, Heck S, Parinthawong N, Metraux J-P. EDS5, an essential component of salicylic acid-dependent signaling for disease resistance in Arabidopsis, is a member of the MATE transporter family. Plant Cell, 2002, 14: 275-286
236.Nelson JC, Van Deynez AE, Autrique E, Sorrells ME, Lu YH, Negre S, Bernard M, Leroy P. Molecular mapping of wheat: homoeologous group 3. Genome, 1995, 38: 525-533
237.Nevo E, Beiles A, Krugman T. Natural selection of allozyme polymorphisms: a microgeographic climatic differentiation in wild emmer wheat (Triticum dicoccoides). Theor. Appl. Genet., 1988b, 75: 529-538
238.Nevo E, Beiles A, Krugman T. Natural selection of allozyme polymorphisms: a microgeographical differentiation by edaphic, topographical, and temporal factors in wild emmer wheat (Triticum dicoccoides) . Theor. Appl. Genet., 1988c, 76:737-752
239.Nevo E, Beiles A. Genetic diversity of wild emmer wheat in Israel and Turkey: Structure, evolution and application in breeding. Theor. Appl. Genet., 1989, 77: 421-455
240.Nevo E, Carver BF, Beiles A. Photosynthetic performance in wild emmer wheat, Triticum dicoccoides: ecological and genetic predictability. Theor. Appl. Genet., 1991b, 81:445-460
241.Nevo E, Golenberg E, Beiles A. Genetic diversity and environmental associations of wild wheat, Triticum dicoccoides, in Israel. Theor. Appl. Genet., 1982,62: 241-254
242.Nevo E, Nishikawa K, Furuta Y, Gonokami Y, Beiles A. Genetic polymorphisms of a- and β-amylase in wild emmer wheat, Triticum dicoccoides, in Israel. Theor. Appl. Genet, 1993,85: 1029-1042
243.Nevo E, Noy-Meir I, Beiles A, Krugman T, Agami M. Natural selection of allozyme polymorphisms: mocro-geographical spatial and temporal ecological differentiations in wild emmer wheat. Israel J. Botany, 1991a, 40: 419-449
244.Nevo E, Pagnotta MA, Beiles A, Porceddu E. Wheat storage proteins: glutenin diversity in wild emmer, Triticum dicoccoides, in Israel and Turkey. 3. Environmental correlates and allozymic associations. Theor. Appl. Genet., 1995, 91: 415-420
245.Nevo E, Payne PI. Wheat storage proteins: glutenin diversity in wild emmer, Triticum dicoccoides, in Israel and Turkey. 1. Geographical patterns and ecological predictability. Theor. Appl. Genet., 1987, 74: 827-836
246.Nevo E. Beiles A. and Storch N. 1983.Microgeographic edaphic differentiation in hordein polymorphisms of wild barley.Theor .Appl.Genet, 64:123 132
247.Nevo E. Evolution of genome-phenome diversity under environmental stress. Proc. Natl. Acad. Sci. U.S.A., 2001, 98: 6233-6240
248.Nevo E. Genetic diversity in nature: patterns and theory. Evol. Biol., 1988a, 23: 217-246
249.Nevo E. Molecular evolution and ecological stress at global, regional and local scales: the Israeli perspective. J. Exp. Zool, 1998,282: 95-119
250.Newton CR, Graham LE, Heptinstall LE, Powell SJ, Summers C, Kalsheker N, Smith JC, Markham AF. Analysis of any point mutation in DNA. The amplification refractory mutation system (ARMS) . Nucleic Acids Research, 1989,17: 2503-2516
251.Nickerson DA, Taylor SL, Weiss KM, et al. DNA sequence diversity in a 9.7 kb region of the human lipoprotein lipase gene. Nature Genetics, 1998,19: 233-240
252.Nightingale MJ, Marchylo BA, Clear RM, Dexter JE, Preston KR. Fusarium head blight: effect of fungal proteases on wheat storage proteins. Cereal Chem., 1999, 76: 150-158
253.Nishiuchi T., Masuda D., Nakashita H., Ichimura K., Shinozaki K., Yoshida S., Kimura M., Yamaguchi I., Yamaguchi K. Fusarium phytotoxin trichothecenes have an elicitor-like activity in arabidopsis thaliana, but the activity differed significantly among their molecular species. MPMI 2006,19: 512-520
254.Oda Y, Matsunaga T, Fukuyama K, Miyazaki T, Morimoto T. Tertiary and quaternary structures of 0.19 alpha-amylase inhibitor from wheat kernel determined by X-ray analysis at 2.06 A resolution. Biochemistry, 1997, 36 (44) : 13503-13511
255.Ogihara Y, Tsunewaki K. Diversity and evolution of chloroplast DNA in Triticum and Aegilops as revealed by restriction fragment analysis. Theor. Appl. Genet., 1988, 76: 321-332
256.Okuda M, Satoh T, Sakurai N, Shibuya K, Kaji H, Samejima T. Overexpression in Escherichia coli of chemically synthesized gene for active 0.19 alpha-amylase inhibitor from wheat kernel. J. Biochemistry, 1997, 122 (5) : 918-926
257.Oliver PE, Cai X, Xu SS, Chen X, Stack RW. Wheat-Alien Species Derivatives: A Novel Source of Resistance to Fusarium Head Blight in Wheat. Crop Sci. 2005, 45: 1353-1360
258.Oliver R, Osbourn A. Molecular dissection of fungal phytopathogenicity. Microbiol., 1995, 141: 1-9
259.Olivera PE, Steffenson B, Anikster Y. Reaction of Aegilops sharonesis to Fusarium head blight. In: S.C. Canty, J. Lewis & R.W. Ward (Eds.) , 2003 National Fusarium Head Blight Forum Proc, U.S. Wheat & Barley Scab Initiative, 2003, pp. 226 (abstract) .
260.Orth RA, Bushuk W. A comparative study of the proteins of wheat of diverse baking quality. Cereal Chem., 1972,49: 268-275.
261.Orth RA, Bushuk W. Studies on glutenin: 11 Relation of variety location of growth and baking quality to molecular weight distribution of subunits.Cereal Chem., 1973, 50: 191-197
262.Osbeme TB. The protein of the wheat kernel. Mashington: publication of the Carnegie institute, 1907
263.Pagnotta MA, Nevo E, Beiles A, Porceddu E. Wheat storage proteins: glutenin diversity in wild emmer, Triticum dicoccoides, in Israel and Turkey. 2. DNA diversity detected by PCR. Theor. Appl. Genet., 1995, 91: 409-414.
264.Pandya MJ, Sessions RB, Williams PB, Dempsey CE, Tatham AS, Shewry PR, Clarke AR. Structural characterization of a methionine-rich, emulsifying protein from sunflower seed. Proteins, 2000, 38 (3) : 341-349
265.Parry DW, Jenkinson P, McLeod L. Fusarium ear blight (scab) in small grain cereals- a review. Plant Pathol., 1995, 44: 207-238
266.Payan F. Structural basis for the inhibition of mammalian and insect α-amylase by plant inhibitors. Biochim. Biophys. Acta, 2004,1696: 171-180
267.Payne PI, Corfield KG. Subunit composition of wheat glutenin proteins isolated by gel filtration in a dissociating medium. Planta, 1979,145: 83-33
268.Payne PI, Lawrence GJ. Catalogue of alleles for the complex gene loci, Glu-A1, Glu-B1 and Glu-D\ which code for high molecular weight subunits of glutenin in hexaploid wheat. Cereal Res Commun, 1983,11: 29-35
269.Payne PI. Genetics of wheat storage proteins and the effect of allelic variation on breadmaking quality. Annu Rev Plant Physiol., 1987b, 38: 141-153
270.Payne PL The relationship between HMW glutein subunit composition and the bread-making quality of British-grown wheat varieties. J. Sci. Food Agric, 1987a, 40: 51-65
271.Pekkarinen AI, Jones BL. Trypsin-like proteinase produced by Fusarium culmorum grown on grain proteins. J. Agric. Food Chem., 2002, 50: 3849-3855
272.Peraica M, Radic B, Lucic A, Pavlovic M. Toxic effects of mycotoxins in humans. Bull. World Health Org., 1999, 77: 754-766
273.Petersen G, Seberg O, Yde M, Berthelsen K. Phylogenetic relationships of Triticum and Aegilops and evidence for the origin of the A, B, and D genomes of common wheat (Triticum aestivum) . Mol. Phyl. Evol., 2006, 39: 70-82
274.Petrucci T, Rab A, Tomasi M, Silano V. Further characterization studies of the α-amylase protein inhibitor of gel electrophoretic mobility 0.19 from wheat kernel. Biochim. Biophys. Acta, 1976,420: 288-297
275.Petrucci T, Sannia G, Parlamenti R, et al. Structural studies of wheat monomeric and dimeric protein inhibitors of alph a-amylase. Biochem J., 1978, 173: 229-235
276.Petrucci T, Tomasi M, Cantagalli P, Silano V. Comparison of wheat albumin inhibitors of a-amylase inhibitors and trypsin. Phytochemistry, 1974,13: 2487-2495
277.Pfeil T, Schwabl U, Ulmer WT, Konig W. Western blot analysis of water-soluble wheat flour (Triticum vulgaris) allergens. Int. Allergy. Appl. Immunol., 1990, 91: 224-231
278.Picoult-Newberg L, Ideker TE, Pohl MG, Taylor SL, Donaldson MA, Nickerson DA, Boyce-Jacino M. Mining SNPs from EST databases, Genome Research, 1999, 9: 167-174
279.Placinta CM, D'Mello JPF, Macdonald AMC. A review of worldwide contamination of cereal grains and animal feed with Fusarium mycotoxins. Anim. Feed Sci. Technol., 1999,78:21-37
280.Poerio E, Caporale C, Carrano L, Pucci P, Buonocore V. Assignment of the five disulphide bridges in an alphα-amylase inhibitor from wheat kernel by FAB/MS and Edman degradation. Eur. J. Biochem., 1991, 199: 595-600
281.Posada D, Crandall KA. Modeltest: Testing the model of DNA substitution. Bioinformatics, 1998, 14: 817-818
282.Posch A, Weiss W, Wheeler C, Dunn MJ, Gorg A. Sequence analysis of wheat grain allergens separated by two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis, 1995,16: 1115-1119
283.Prakash B, Selvaraj S, Murthy MRN, Sreerama YN, Rajagopal RD, Gowda LR. Analysis of the amino acid sequences of plant Bowman-Birk inhibitors. J. Mol. Evol., 1996,42:560-569
284.Price J, Root T. Ecosystem Functioning Under Abrupt Climate Change, Potential Surprises and Adaptations. 2004, [http://www.accstrategy.org/presentdrafts.html]
285.Pritsch C, Vance CP, Bushnell WR, Somers DA, Hohn TM, Muehlbauer GJ. Systemic expression of defense response genes in wheat spikes as a response to Fusarium graminearum infection. Physiol. Mol. Plant Pathol., 2001, 58: 1-12
286.Rafalski A. Aplications of single nucleotide polymorphisms in crop genetics, Plant Biology, 2002a, 5:94-100
287.Rafalski A. Novel genetic mapping tools in plants: SNPs and LD-based approaches. Plant Sci., 2002b, 162: 329-333
288.Rahman S, Jolly CJ, Skerritt JH, Wallosheck A. Cloning of a wheat 15 kDa grain softness protein (GSP) . GSP is a mixture of puroindoline-like polypeptides. Eur. J. Biochem., 1994,223:917-925
289.Rambaut A, Drummond A. 2005, Tracer, available at http://evolve.zoo.ox.ac.uk
290.Raskina O, Belyyev A, Nevo E. Repetitive DNAs of wide emmer wheat Triticum dicocoides and their relation to S-genome species: molecular-cytogenetic analysis. Genome, 2002,45 (2) : 391-401
291.Reddy P, Appels R. Analysis of a genomic DNA segment carrying the high-molecular wheat (HMW) glutenin Bx 17 subunit and its use as an RFLP marker. Theor. Appl. Genet., 1993, 85: 616-624
292.Remington DL, Thornsberry JM, Matsuoka Y, Wilson LM, Whitt SR, Doebley J, Kresovich S, Goodman MM, Buckler ES. Structure of linkage disequilibrium and phenotypic associations in the maize genome.Proc Natl Acad Sci U.S.A.,2001,98:11479-11484
293.Riichiro U,Ayako N,Shoichi T,Kouichi K,Koichiro T,Nobuyuki Y.New enzymatic synthesis of 63-modified maltooligosaccharides and their inhibitory activities for human α-amylases.Carbohydrate Research,1998,307:69-76
294.Ryals JA,Neuenschwander UH,Willits MG,Molina A,Steiner H-Y,Hunt MD.Systemic acquired resistance.Plant Cell,1996,8:1809-1819
295.Ryan CA,Pearce G.Systemin:a polypeptide signal for plant defensive genes.Annu.Rev.Cell Dev.Biol.,1998,14:1-17
296.Ryan CA.Protease inhibitors in plants:genes for improving defenses against insect and pathogens.Annu.Rev.Phytophathol,1990,28:425-449
297.Ryan CA.The systemin signaling pathway:differential activation of plant defensive genes.Biochim.Biophys.Acta,2000,1477:112-121
298.Sales MP,Gerhardt IR,Grossi-de-SaA MF,Xavier-Filho J.Do legumes storage proteins play a role in defending seeds against bruchids? Plant Physiol.,2000,124,515-522
299.Sampson HA.Food allergy.I.Immunopathogenesis and clinical disorders.J.Allergy Clin.Immunol.,1999,103:717-728
300.Sanchez-Monge R,Barber D,Mendez E,Garcia-Olmedo F,Salcedo G.Gene encoding alpha-amylase inhibitors are located in the short arms of Chromosomes 3B,3D and 6D of wheat(Triticum aestivum L.).Theor.Appl.Genet.,1986,72:108-113
301.Sanchez-Monge R,Garcia-Casado G,Lopez-Otin C,Armentia A,Salcedo G.Wheat flour peroxidase is a prominent allergen associated with baker's asthma.Clin.Exp.Allergy,1997,27:1130-1137
302.Sanchez-Monge R,Gomez L,Barber D,Lopez-Otin C,Armentia A,Salcedo G.Wheat and barley allergens associated with baker's asthma.Glycosylated subunits of the alpha-amylase-inhibitor family have enhanced IgE-binding capacity.Biochem.J.,1992,281:401-405
303.Sanchez-Monge R,Gomez L,Garcia-Olmedo F,Salcedo G.New dimeric inhibitor of heterologous alpha-amylase encoded by a duplicated gene in the short arm of chromosome 3B of wheat(Triticum aestivum L.).European Journal of Biochemistry,1989,183:37-40
304.Sander I,Merget R,Degens PO,Goldscheid N,Bruning T,Raulf-Heimsoth M. Comparison of wheat and rye flour skin prick test solutions for diagnosis of baker's asthma. Allergy, 2004, 59 (1) : 95-98
305.Sander I, Merget R, Leonhardt L, Chen Z, Raulf-Heimsoth M, Baur X. Prevalence of allergen-specific IgE in baker's asthma. Allergy, 1999, 54 (Suppl 52) : 50-51
306.Sandiford CP, Tatham AS, Fido R, Welch JA, Jones MG, Tee RD, et al. Identification of the major water/salt insoluble wheat proteins involved in cereal hypersensitivity. Clin. Exp. Allergy, 1997, 27: 1120-1129
307.Sano H, Ohashi Y. Involvement of small GTP-binding protein in defense signal-transduction pathways of higher plants. PNAS, 1995, 92: 4138-4144
308.Sasanuma T, Miyashita NT, Tsunewaki K. Wheat phylogeny determined by RFLP analysis of nuclear DNA. 3. Intra- and interspecific variations of five Aegilops Sitopsis species. Theor. Appl. Genet., 1996, 92: 928-934
309.Schroeder HE, Gollasch S, Moore A, Tabe LM, Craig S, Hardie DC, Chrispeels MJ, Spencer D, Higgins TJV. Bean [alpha]-Amylase Inhibitor Confers Resistance to the Pea Weevil (Bruchus pisorum) in Transgenic Peas (Pisum sativum L.) Plant Physiol, 1995,107: 1233-1239
310.Schroeder HW, Christensen JJ. Factors affecting resistance of wheat to scab caused by Gibberella zeae. Phytopathology, 1963, 53: 831-838
311.Schuler HT, Poppy MG, Kerry BR, Denholm L. Insect-resistant transgenic plants. Trends Biotechnol., 1998, 16: 168-174
312.Schwarz G, Sift A, Wenzel G, Mohler V. DHOLC scoring of a SNP between promoter sequences of HMW glutenin x-type alleles at the Glu-D1 locus in wheat. Journal of Agricultural and Food Chemistry, 2003, 51: 4263-4267
313.Schwarz PB. Impact of Fusarium head blight on malting and brewing quality of barley. In: Leonard, K.J. & Bushnell W.R. (eds.) Fusarium Head Blight of Wheat and Barley. St Paul: American Phytopathological Society Press, 2003, Pp. 395-419
314.Shen X, Kong L, Ohm H (2004) Fusarium head blight resistance in hexaploid wheat (Triticum aestivum) -Lophopyrum genetic lines and tagging of the alien chromatin by PCR markers. Theor. Appl. Genet., 108: 808-813
315.Shen X, Ohm H. Fusarium head blight resistance derived from Lophopyrum elongatum chromosome 7E and its augmentation with Fhb1 in wheat. Plant Breeding 2006,125: 424-429
316.Shen X, Ohm H. Molecular mapping of Thinopyrum-derived Fusarium head blight resistance in common wheat. Mol Breeding, 2007,20:131-140
317.Shewry PR, Halford NG, Belton PS, Tatham AS. The structure and properties of gluten: an elastic protein from wheat grain. Philosophical Transactions of the Royal Society of London: Biological Sciences, 2002c, 357: 133-142
318.Shewry PR, Halford NG, Tatham AS. High-molecular-weight subunits of wheat glutenin. J. Cereal Sci., 1992, 15: 105-120
319. She wry PR, Halford NG Cereal seed storage proteins: structures,properties and role in grain utilization. Journal of Experimental Botany, 2002a, 53: 947-958.
320.Shewry PR, Miflin BJ. Seed storage proteins of economically important cereals. Adv. Cereal Sci. Technol., 1985, 7:1 -84
321 .Shewry PR, Pandya MJ. in Seed Proteins (Shewry, P. R. and Casey, R., eds), 1999, pp. 563-586, Kluwer Academic Publishers, Dordrecht
322.Shewry PR, Tatham AS, Barro P, Lazzeri P. Biotechnology of bread making: unraveling and manipulating the multi-protein gluten complex. Bioltechnology, 1995b, 13:1185-1190
323.Shewry PR, Tatham AS. Disulphide Bonds in Wheat Gluten Proteins. Journal of Cereal Science, 1997,25: 207-227
324.Shewry PR. Beaudoin F. Jenkins J. Griffiths-Jones S. Mills NC. Plant protein families and their relationships to food allergy. Biochemical Society Transactions 2002b, 30: 906-910
325.Shewry PR. Napier JA. Tatham AS. Seed storage proteins: structures and biosynthesis. The Plant Cell, 1995a, 7: 945-956
326.Shewry PR. Plant storage proteins. Biol. Rev., 1995, 70: 375-426
327.Sicherev SH, DeSimone J, Furlong TJ. Peanut and tree nut allergic reactions in restaurants and food establishments. J. Allergy Clin. Immunol., 2001,108: 867-870
328.Silano V, Poerio E, Buonocore V. A model for the interaction of wheat monomeric and dimeric protein inhibitors with α-amylase. Molecular and Cellular Biochemistry, 1977, 18:87-91
329.Silva MC, Da M, Grossi de sa MF, Chrispeels MJ, Togawa RC, Neshich G. Analysis of structural and physico-chemical parameters involved in the specificity of binding between α-amylases and their inhibitors. Protein Engineering, 2000, 13:167-177
330.Simeone M, Gedye K, Mason-Gamer R, Gill B, Morris C. Conserved regulatory elements identified from a comparative puroindoline gene sequence survey of Triticum and Aegilops diploid taxa. J. Cereal Sci., 2006, 44: 21-33
331.Singh J, Appels R, Sharp PJ, Sherritt JH: Albumin polymorphism and mapping of a dimeric α-amylase inhibitor in wheat. Aust. J. Agric. Res., 2001b, 52: 1173-1179
332.Singh J, Blundell M, Tanner G, Skerritt JH. Albumin and globulin proteins of wheat flour: immunological and N-terminal sequence characterization. Journal of Cereal Science, 2001a, 34:85-103
333.Singh J, Skerritt JH. Chromosomal control of albumins and globulins in wheat grain assessed using different fractionation procedures. Journal of Cereal Science, 2001, 33: 163-181
334.Singh NK, Shepherd KW. Solubility behavior, synthesis, degradation and subcellular location of a new class of disulphide-linked proteins in wheat endosperm. Australian Journal of Plant Physiology, 1987, 14: 245-252
335.Smith-Huerta NL, Huerta AJ, Barnhart D, Waines JG. Genetic variation in wild diploid wheats Triticum monococcum var. boeoticum and T. urartu (Poaceae) . Theor. Appl. Genet, 1989, 78: 260-264
336.Solemimani VD, Baum BR, Johnson DA. Efficient validation of single nucleotide polymorphisms in plants by Allele-Specific PCR, with an example from Barley, Plant Molecular Biology Reporter, 2003, 21: 281-288
337.Somers DJ, Kirkpatrick R, Moniwa M, Walsh A. Mining single-nucleotide polymorphisms from hexaploid wheat ESTs. Genome, 2003,46: 431-437
338.Sommer SS, Groszbar AR, Bottema CDK. PCR amplification of specific alleles (PASA) is a general method for rapidly detecting known single base-pair changes. Biotechniques, 1992, 12: 82-87
339.Spena A, Viotti A, Pirrota V. A homologous repetitive block structure underlies the heterogeneity of heavy and light chain zein genes. EMBO J., 1982, 1: 1589-1594
340. StatSoft, Inc. Electronic Statistics Textbook. Tulsa, OK: StatSoft. [http://www.statsoft.com/textbook/stathome.html] 2006.
341.Steffenson BJ. Fusarium head blight of barley: impact, epidemics, management, and strategies for identifying and utilizing genetic resistance. In: Leonard, K.J. & Bushnell W.R. (eds.) Fusarium Head Blight of Wheat and Barley. St Paul: American Phytopathological Society Press, 2003. Pp. 241-295
342.Stoessl A, Unwin CH. The antifungal factors in barley. V. Antifungal activity of the hordatines. Can. J. Bot., 1978,48: 465-470
343.Stone PJ, Nicolas ME. Varietals differences in mature protein composition of wheat resulted from different rates of polymeraccumulation during grain filling, Aust. J. Plant Physiol., 1996,23: 727-737
344.Strobl S, Muhlhahn P, Bernstein R, Wiltscheck R, Maskos K, Wunderlich M, Huber R, Glockshuber R, Holak TA. Determination of the three-dimensional structure of the bifunctional alpha-amylase/trypsin inhibitor from ragi seeds by NMR spectroscopy. Biochemistry, 1995, 34: 8281-8293
345.Struck C, Mueller E, Martin H, Lohaus G The Uromyces fabae UfAAT3 gene encodes a general amino acid permease that prefers uptake of in planta scarce amino acids. Molecular Plant Pathology, 2004, 5: 183-189
346.Svensson B. Fukuda K. Nielsen PK. B0nsager.BC. Proteinaceous a-amylase inhibitors. BBA, 2004, 1696:145-156
347.Swofford DL. PAUP~*. Phylogenetic Analysis Using Parsimony ( ~*and Other Methods) . Version 4. Sinauer Associates, Sunderland. Massachusetts. 1998
348.Tai SSK, Wu LSH, Chen ECF, Tzen JTC. Molecular cloning of 11S globulin and 2S albumin, the two major seed storage proteins in sesame. J. Agric. Food Chem., 1999, 47:4932-4938
349.Takase K. Site-directed mutagenesis reveals critical importance of the catalytic site in binding of a-amylase by wheat proteinaceous inhibitor. Biochemistry, 1994, 33: 7925-7930
350.Talbert LE, Blake TK, Storlie EW, Levin M. Variability in wheat based on low-copy DNA sequence comparisons, Genome, 1995,38: 951-957
351.Tao HP, Adalsteins AE, Kasarda DD. Intermolecular disulfide bonds link specific high-molecular-weight glutenin subunits in wheat endosperm. Biochimica Biophysica Acta, 1992,1159: 13-21
352.Tenaillon MI, Swakins MC, Long AD, Gaut RL, Doebley JF, Gaut BS. Patterns of DNA sequence polymorphism along chromosome 1 of maize (Zea mays ssp. mays L.) . Proc. Natl. Acad. Sci. U.S.A., 2001,98: 9161-9166.
353.Terras FRG, Schoofs HME, Thevissen K, Osborn RW, Vanderleyden J, Cammue BPA, Broekaert WF. Synergistic enhancement of the antifungal activity of wheat and barley thionins by radish and oilseed rape 2S albumins and by barley trypsin inhibitors. Plant Physiol., 1993, 103: 1311-1319
354.Thouraya M, Emmanuelle B. Proteomic analysis of the efect of heat stress on hexaploid wheat grain: Characterization of heat-responsive from non-prolamins fraction. Proteomics, 2004,4: 505-513
355.Tiffin P, Gaut BS. Molecular Evolution of the Wound-Induced Serine Protease Inhibitor wipl in Zea and Related Genera. Mol. Biol. Evol., 2001, 18: 2092-2101
356.Titarenko E, Chrispeels MJ. cDNA cloning, bio-chemical characterization and inhibition by plant inhibitors of the α-amylases of the Western corn rootworm, Diabrotica vir-gifera virgifera. Insect Biochem. Mol. Biol., 2000, 30: 979-990
357.Tuleen NA, Hart GE. Isolation and characterization of wheat-Elytrigia elongata chromosome 3E and 5E addition and substitution lines. Genome, 1988, 30: 519-524
358.Van Slageren MW. Wild wheats: a monograph of Aegilops L. and Amblyopyrum (Jaub. & Spach) Eig (Poaceae). Wageningen Agric Univ Press, Wageningen 1994
359.Vittozzi L, Morisi G, Silano V. Continuous automated assay of alphα-amylase inhibitors. J. Sci. Food Agric, 1976,27 (5) : 449-453
360.Walia H, Wilson C, Wahid A, Condamine P, Cui X, Close TJ. Expression analysis of barley (Hordeum vulgare L.) during salinity stress. Func. Integr. Genomics, 2006,6: 143-156
361.Walsh BJ, Howden ME. A method for the detection of IgE binding sequences of allergens based on a modification of epitope mapping. J. Immunol. Methods, 1989, 121:275-280
362.Walton JD. Deconstructing the cell wall. Plant Physiol., 1994, 104: 1113-1118
363.Wan Y, Wang D , Shewry PR, Halford NG. Isolation and characterization of five novel high molecular weight subunit of glutenin genes from Triticum timopheevi and Aegilops cylindrica. Theor Appl Genet, 2002,104: 828-839
364. Wan YF, Yen C, Yang JL. The diversity of head-scab resistance in Triticeae and their relation to ecological conditions. Euphytica, 1997, 97: 277-281
365.Wang DG, Fan JB, Siao CJ, Berno A, Young P, Sapolsky R, Ghandour G, Perkins N, Winchester E, Spencer J, Kruglyak L, Stein L, Topaloglou T, Hubble E, Robinson E, Mittmann M, Morris MS, Shen N, Kilburn D, Rioux J, Nusbaum C, Rozen S, Hudson TJ, Lander ES. Large-scale identification, mapping, and genotyping of single-nucleotide polymorphisms in the human genome. Science, 1998, 280: 1077-1082
366.Wang JR, Wei YM, Yan ZH, Nevo E, Baum BR, Zheng YL. Molecular characterization of dimeric a-amylase inhibitor genes in wheat and development of genome allele-specific primers for the genes located on chromosome 3BS and 3DS. Journal of Cereal Science 2006b 43:360-368
367.Wang JR, Wei YM, Yan ZH, Zheng YL. Detection of single nucleotide polymorphisms in the 24 kDa dimeric α-amylase inhibitors from cultivated wheat and its diploid putative progenitors. Biochim. Biophys. Acta 2005 1723: 309-320
368.Wang JR, Wei YM, Yan ZH, Zheng YL. Genetic mapping of the wheat dimeric α-amylase inhibitor multi-gene family using allele-specific primers based on intergenomic SNPs. Plant molecular biology report, 2006c, 4 (3) : 1-8
369.Wang JR, Wei YM, Yan ZH, Zheng YL. SNP analysis and haplotype identification of the genes encoding monomeric a-amylase inhibitors. Genetica 2008, (online)
370.Wang JR, Yan ZH, Wei YM, Zheng YL. A novel HMW glutenin subunit gene Ee1.5 from Elytrigia elongata (Host) Nevski. Journal of cereal Science, 2004, 40: 289-294
371.Wang JR, Yan ZH, Wei YM, Zheng YL. Characterization of High-Molecular-Weight Glutenin Subunits Genes from Elytrigia elongata (Host) Nevski. Plant Breeding 2006a 125: 89-95
372.Wang JR, Zhang L, Wei YM, Yan ZH, Baum BR, Nevo E, Zheng YL. Sequence polymorphisms and relationships of dimeric a-amylase inhibitor genes in the B genomes of Triticum and S genomes of Aegilops. Plant Science, 2007a, 173: 1-11
373 .Wang L, Zhang K, Zhang L. Perfect phylogenetic networks with recombination. J. Comp. Bio., 2001, 8: 69-78
374.Wang RR-C, von Bothmer R, Dvorak J, Fedak G, Linde-Laursen I, Muramatsu M. Genome symbols in the Triticeae (Poaceae), in Proc. 2nd Int. Triticeae Symp., edited by Wang RR-C, Jensen KB, Jaussi C, Utah State Univ., Logan 1996, pp. 29-34
375.Warchalewski JR, Gralik J, Winiecki Z, et al. The effect of wheat a-amylase inhibitors incorporated into wheat-based artificial diets on development of Sitophilus granarius L., Tribolium confusum Duv., and Ephestia kuehniella Zell. J. Appl. Ent., 2002, 126: 161-168
376.Weegels PL, Hamer RJ, Schofield JD. Functional properties of wheat glutenin. Cereal Sci., 1996,23:1-1
377.Weiss W, Huber G, Engel KH, Pethran A, Dunn MJ, Gooley AA, et al. Identification and characterization of wheat grain albumin/globulin allergens. Electrophoresis, 1997, 18:826-33
378.Wendel JF. Genome evolution in polyploids. Plant Molecular Biology, 2000, 42: 225-249
379.Whitaker JR. a-amylase inhibitors of higher plants and microorganisms. in: J.E. Kinsella, W.G. Soucie (Eds.) , Proc. Protein Co-prod. Symp., Am. Oil Chem. Soc, Champaign, IL, 1988, pp. 354-380
380.Wieser H, Antes S, Seilmeier W. Quantitative determination of gluten protein types in wheat flour by reversed-phase high performance liquid chromatography. Cereal Chem, 1998,75 (5) : 644-650
381.Wiesner H, Seilmeier W, Belitz HD. Vergleichende Untersuehungen uber partiele Aminosauresequenzen yon Prolaminen und Glutelinen versehriedener Getreidearten. Z. bensm Unters Forseh, 1980, 170: 17
382.Wisniewska H, Chelkowski J. Influence of exogenic salicylic acid on Fusarium seedling blight reduction in barley. Acta Physiologiae Plantarum, 1999, 21: 63-66
383.Worland AJ, Gale MD, Law CN. wheat genetics. Wheat breeding, 1987, 2(1): 129-171
384.Wrigley CW. Giant protein with flour power. Nature, 1996, 381: 738-739
385.Xiao SH, Zhang XY, Yan CS, Lin H Germplasm improvement for pre-harvest sprouting resistance in Chinese white-grained wheat: An overview of the current strategy. Euphytica, 2002,126: 35-38
386.Xie DX, Freys BF, James S, Nieto-Rostro M, Tuner JG. COI1: an Arabidopsis gene required for jasmonate-regulated defence and ferticility. Science, 1998, 280: 1091-1094.
387.Yan ZH, Wan YF, Liu KF, Zheng YL, Wang DW. Identification of a novel HMW glutenin subunit and comparison of its amino acid sequence with those of homologous subunits. Chinese Science Bulletin., 2002,47 (3) : 220-225
388.Yan ZH, Wei YM, Wang JR, L DC, Dai SF, Zheng YL. Characterization of two HMW glutenin subunit genes from Taenitherum Nevski. Genetica, 2006, 127: 267-276
389.Yeh FC, Yang RC, Boyle T, Ye ZH, Mao JX. POPGENE, the user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Center, University of Alberta, Canada. 1977
390.Zawistowska U, Bietz JA, Bushuk W. Characterization of low-molecular-weight protein with high aflqnity for flour lipid from twowheat classes. Cereal Chem, 1986, 63: 414-419
391.Zhang H, Reader SM, Liu X, Jia JZ, Gale MD, Devos KM. Comparative genetic analysis of the Aegilops longissima and Ae. sharonensis genomes with common wheat, Theor.Appl.Genet.,2001,103:518-525
392.Zhang LQ,L.Dengcai,Y.Zehong,L.Xiujin,Z.Youliang,Z.Yonghong,Rapid changes of microsatellite flanking sequence in the allopolyploidization of new synthesized hexaploid wheat.Science in China Series C-Life Sciences,2004,47:553-561
393.Zheng Z,Mosher SL,Fan B,Klessig DF,Chen Z.Functional analysis of Arabidopsis WRKY25 transcription factor in plant defense against Pseudomonas syringae.BMC Plant Biol.2007,7:2
394.冯英,薛庆中.抗虫基因工程及其安全性.遗传,2001,23:571-576
395.陆维忠,程顺和,王裕中主编.小麦赤霉病研究,科学出版社2001,序
396.毛伯韧,吴兆苏.小麦种子休眠特性的遗传及其机理研究.中国农业科学,1983,6:53-58
397.王爱丽,裴玉贺,张倩,晏月明,小麦非醇溶性蛋白(non.prolamins)的研究进展.首都师范大学学报(自然科学版),2006,27:68-74
398.肖世和,陈孝,徐慧君,林志珊,杜丽璞.大麦α-淀粉酶抑制基因对小麦α-淀粉酶的抑制作用研究.作物学报,1998,24:763-767
399.肖世和,戴大庆,蒋华仁.抗收获前发芽小麦品种的初步研究.四川农业大学学报,1986,4:219-224
400.杨学举,卢少源,张荣芝.小麦籽粒蛋白质组分与面包烘焙品质性状关系的研究.中国粮油学报,1999,14:1-5
401.曾雄生.论小麦在古代中国之扩张.中国饮食文化,2005,1:1
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