用户名: 密码: 验证码:
半夏对遮阴和高温响应机理的研究
详细信息    本馆镜像全文|  推荐本文 |  |   获取CNKI官网全文
摘要
半夏(Pinellia ternate (thunb.) Breit.)为天南星科半夏属植物,以干燥块茎入药,属大宗中药材,需求量较大。为满足需求,各地开始引种栽培,但人工栽培长期以来产量低而不稳。半夏栽培研究越来越受重视。本实验以三个不同生态类型产区(江苏省泰州市、山东省潍坊市、河北省安国市)来源的栽培半夏为材料,在同一环境条件下栽培,研究半夏对遮阴和高温胁迫的生理响应,及遮阴对半夏块茎产量和总生物碱含量的影响。遮阴试验设置四个遮阴处理:0%、30%、50%、70%,遮阴度通过不同密度遮阳网调节,将三来源半夏种植于同一试验地。高温试验设置四个温度处理(昼/夜):25℃/20℃、30℃/20℃、35℃/20℃、40℃/20℃,材料种植于苗木育苗盘中,待叶片展开后移入光照培养箱进行温度处理。主要结论:
     1.随着遮阴程度的增加,半夏倒苗期持续时间增长。不同来源半夏倒苗持续时间亦有差异,山东和江苏半夏的持续时间较短,河北半夏的较长。
     2.遮阴显著降低了半夏的总产量,但有利于半夏珠芽的产生。随着遮阴程度的增加,一级块茎所占比例显著降低,四级块茎所占比例显著增加。三来源半夏的产量山东>江苏>河北,差异显著。半夏在同一地区连续种植存在品种退化现象,引种时应引用地理生态环境相近产区的半夏资源,以免造成减产。
     3.在0%-50%遮阴处理下,半夏叶片净光合速率日变化呈双峰曲线,有明显的“光合午休”;在70%处理下则呈单峰曲线,避免了“光合午休”。随着遮阴程度的增加,净光合速率日变化最大值降低且出现时间延迟。半夏叶片日光合值和日平均值随遮阴程度增加而降低。三来源半夏均有此表现。随着生育期的推进,光合速率随遮阴程度增加而降低。
     4.遮阴提高了叶片叶绿素及其组分的含量。随着光照强度的增加,半夏叶片出现光抑制,但这种抑制是可逆的,随着午后光照强度的降低,荧光参数均能恢复到早晨时的状态。
     5.遮阴降低了半夏叶片和块茎中可溶性糖和淀粉的含量,其关键酶活性也随遮阴程度增加而降低。同时遮阴处理下可溶性糖的合成和向块茎中的运送受到抑制,使得半夏块茎中淀粉含量降低,影响了半夏的产量形成。
     6.遮阴降低了半夏叶片和块茎中SOD、POD、CAT酶活性,第一次倒苗到第二次出苗间,处理间差异不显著,之前和之后处理间差异显著。生育前期,空气温度和土壤温度较高,随遮阴程度增加叶片和块茎中保护酶活性升高从而使MDA含量较低;生育后期,随遮阴程度增加MDA含量显著增加。
     7.遮阴显著提高了半夏块茎总生物碱含量,但总生物碱产量则随遮阴程度增加而显著减低。生物碱含量与块茎大小有负相关关系,其机理有待进一步研究。半夏总生物碱产量山东半夏>江苏半夏>河北半夏。
     8.随着胁迫温度的升高和时间的延长,半夏叶片的光合速率迅速降低。高温胁迫对光系统产生了不可逆的破坏,加速了叶片的衰老。高温显著降低了最大荧光、最大光化学效率、PSⅡ实际光化学效率。初始荧光、非光化学猝灭随着温度的升高先显著增加,后随叶片衰老而降低。三来源半夏均有此表现。
     9.高温胁迫破坏了半夏叶片和块茎中的保护酶系统。叶片中SOD、CAT酶活性随处理温度的升高和处理时间延长先升高后显著降低,POD酶活性则持续降低;块茎中保护酶活性则均随温度升高和处理时间延长先升高后降低,但块茎中酶活性降低的时间晚于叶片中的。MDA含量随温度升高和处理时间延长而显著增加,前期增加幅度小于后期。三来源半夏均有此表现。
     10.对照处理下半夏叶片和块茎中淀粉含量变化不显著,高温胁迫下淀粉含量显著降低。可溶性糖含量随着胁迫时间的延长先增加后显著降低。高温胁迫下SS、SPS、SSS酶失活,活性显著降低。高温胁迫降低了同化物的积累,从而加剧了其消耗。三来源半夏均有此表现。
Pinellia ternate (thunb.) Breit. is herbaceous monocots of Aareeae Pnielll'a. It is one ofthe numeorus traditional Chinese l herbs,with its tubers used for medicine and there is ahigher requirement for it in recent years.Men has cultivated Pinellia ternate (thunb.) Breit.for30years.The growth of Pinellia ternate (thunb.) Breit. is greatly affected by cultivationconditions.In addition, the cost of cultivation is high and many diseases affect the tuber yieldof Pinellia ternate (thunb.) Breit. The research objects in the paper are Pinellias from threedifferent ecological production areas (Taizhou of Jiangsu, Weifang of Shandong, Anguo ofHebei), cultivated under the same environmental condition.Studies were taken that thePinellia's physiological response to shading and high tempreture stress,and that the effect onmedicinal ingredients of Pinellia ternata Breit. tubers of shading conditions. Shading test setfour shading degrees, as follows:0%,30%,50%,70%, adjusting with different shade nets ofdifferent densities. The research materials were cultivated under the same environmental plots.High temperature test set four temperature treatments (day/night):25°C/20°C,30°C/20°C,35°C/20°C,40°C/20°C.The materials were planted in a seedling nursery andmoved into light incubato until the leaf unfolding for tempreture treatments.The main resultsare as follows:
     1.With the increasing of shading degree, the duration of sprout tumble of the Pinelliaternate (thunb.) Breit. become longer. The result was different in three P. ternata.s fromdifferent areas.The duration of P. ternata.from Shandong and Jiangsu was shorter,and the theduration of P. ternata.from Hebei was longer.
     Shade significantly reduced the total output of Pinellia, but it is conducive to thegeneration of Pinellia bulbils. With the increase of shade, the proportion of first level tuberwas significantly lower, fourth level tubers significantly increased.The output order of Pinelliafrom three sources was Shandong,Jiangsu,Hebei;and the difference was significant. Plantingin the same area continuously cause the degradation of the species. In order to avoidcuts,Breit in producing areas of the similar geographical environment should be selected forthe introduction.
     In0%-50%relatively light intensity conditions, the curve of leaf net photosynthetic dailyvariation of Pinellia ternata(Thunb.) Breit had two peaks and obvious phenomena of middaydepression.In70%relatively light intensity conditions, the diurnal net photosynthetic rate(NPR) exhibited a single peak curve to avoid phenomena of midday depression. Thegreater degree of shading,the maximum of diurnal net photosynthetic rate(NPR) was lowerwith time delay. Leaf total Pn/day and mean Pn/day of Pinellia ternata(Thunb.) Breit wassmaller with degree of shading increaseing. Pinellia ternate (Thunb.) Breit from three sourceshad the same result. With the growth of plant, the photosynthetic rate was lessening with thedegree of shading increasing.
     Shade could improve total chlorophyll content and the value of chlorophyll component.The greater degree of shading,photoinhibition had appeared in Pinellia ternate (Thunb.) Breitleaf. But this photoinhibition was reversible, the state of Chlorophyll fluorescence parametersrestored to its former good condition in the morning with reducing degree of shading aftermidday.
     Shading can reduce the content of soluble sugar and starch in leaves and tubers. And thekey enzyme's activity also decreased along with the increase of shading degree. At the sametime, the synthesis of soluble sugar and the transport to the tubers would be restrained. So itreduced the starch content and influenced the yield.
     Shading reduced the SOD、POD and CAT enzymes' activity of leaves and tubers. Therewas no significant difference in the treatments between the first sprout tumble and the secondtime. In the early time of the growth period, the air temperature and soil temperature werehigher. With the more shading degree leaves and tubers' protection enzymes' activity wouldincrease and the MDA content was lower. And in the later time of the growth period with themore shading degree the MDA content was higher.
     Shading increased the tuber's total alkaloid content significantly, but the total alkaloidyield decreased along with the increase of shading degree. Alkaloid content and tuber's sizehave negative correlation and its mechanism needs further research. Alkaloid yield in thethree places were the relationship that Shandong> Jiangsu> Hebei.
     2.High temperature stress significantly reduced the photosynthetic rate of Pinellia;withthe increase of temperature stress and the extension of time, leaf photosynthetic rate rapidlyreduced. High temperature reduced the content of chlorophyll, exacerbated the decompositionof chlorophyll, caused the light system damage irreversible, leaf senescence accelerating. Thehigh temperature significantly reduced the initial fluorescence,maximal fluorescence,maximum photochemical efficiency and PS Ⅱactual photochemical efficiency.Non-photochemical quenching increased significantly with temperatures rising and thendeclined with leaf senescence.
     High temperature stress destroyed protective enzyme system of the leaves and the tubers.The SOD and CAT enzymes' activity in the leaves would increase at first and thensignificantly reduce with the increasing of the processing temperature and time but the PODenzyme's activity continued to decrease. The protection enzymes' activity of the tubers wouldincrease at first and then reduce with the increasing of the processing temperature and time.But the activity of the enzyme was reducing later in tubers than in leaves. MDA's contentincreased significantly with the processing temperature and time. The increasing rate was lessin the early time than late. The there sources were all having this performance.
     High temperature significantly reduced the starch content in the Pinellia leaves andtubers.soluble sugar content increased first and then reduced significantly and SS, SPS, SSSactivity significantly reduced. High temperature stress reduces the accumulation ofassimilates, exacerbating their consumption, resulting in lower yields.
引文
安慧,上官周平.光照强度和氮水平对白三叶幼苗生长与光合生理特性的影响[J].生态学报,2009,29(11):6017-6024
    白岗栓,杜社妮,雒聪,等.仁用杏早春遮阴对开花坐果的影响[J].园艺学报,2005,32(6):985-989
    陈佰鸿,曹孜义,赵长增,等.温度和光照对‘红地球’葡萄试管苗光合特性的影响[J].园艺学报,2009,36(4):571-576
    陈华新,安沙舟,李卫军,等.NaCl胁迫增强杂交酸模(Rumex K-l)幼苗叶片光系统Ⅱ的耐热性[J].植物生理与分子生物学学报,2004,30(3):345-350.
    陈顺钦,袁媛,罗毓健,等.光照对黄芩黄酮类活性成分积累及其相关基因表达的影响[J].中国中药杂志,2010,35(6):682-685
    陈效杰.半夏优化栽培模式研究初报[J].甘肃农业科技,1998,4(4):19
    陈燕,郑小林,曾富华,等.高温干旱下两种冷季型草坪草叶片细胞超微结构的变化[J].西北植物学报,2003,23(2):304-308
    陈贻竹,彭长连.盐藻的叶绿素荧光测定.中科院华南植物所集刊,1994(9):45-101
    邓素芳,杨旸,赖钟雄.朱砂根愈伤组织分化根的研究[J].中国农学通报,2010,26(6):189-192
    杜娟,马小军,李学东.半夏不同种质资源AFLP指纹系谱分析及其应用[J].中国中药杂志,2006,31(1):30-33
    杜玮炜,姚小洪,黄宏文.环境胁迫对雷公藤中雷公藤红素含量的影响[J].植物生态学报,2009,33(1):180-185
    杜永臣.园艺作物高温逆境生理的研究进展.园艺学年评,1996,2:1-13
    冯礼斌,谢永江.半夏茎腐病综合治理措施[J].四川农业科技,2008,(7):41
    付振书,赵世杰,孟庆伟,等.高温强光下耐热性不同的两个甘蓝品种幼苗光合作用差异的研究.园艺学报,2005,31(l):25-29.
    龚成文.3种肥料在半夏上的肥效比较试验[J].甘肃农业科技,2000,(5):32-33
    龚成文.半夏综合栽培技术研究[J].中国中药杂志,2005,30(16):1240-1242
    顾德兴,郭巧生.半夏群体生物学特性的研究[J].南京农业大学学报,1990,13(2):11-16
    顾德兴.南京两种半夏群体水平变异式样的比较[J].植物分类学报,1991,29(5):423-430
    郭培国,李荣华.夜间高温胁迫对水稻叶片光合机构的影响.植物学报,2000,42(7):673-678.
    郭巧生,段金廒,贺善安.半夏不同居群3种化学成分的动态比较研究[J].中国中药杂志,2001,26(5):296-299
    郭巧生,贺善安,刘丽.半夏种内不同居群生长节律的研究[J].中国中药杂志,2001,26(4):233-237
    郭巧生,沈文飚,刘丽,等.半夏种内不同居群酯酶和超氧化物歧化酶同工酶酶谱特征分析[J].植物资源与环境学报,2001,10(2):42-46
    郭巧生,王庆亚,史红专.半夏种内不同居群花粉粒形态比较研究[J].中国中药杂志,2006,31(1):27-30
    郭巧生,张国泰,徐来武.不同繁殖材料对半夏产量的影响[J].中国中药杂志,1993,18(3):140-143
    郭巧生,张君毅,易茜茜.半夏不同居群根系活力和生化指标对高温胁迫的响应[J].中国中药杂志,2007,32(15):1508-1510
    郭巧生.半夏研究进展[J].中药研究与信息.2000,2(10):15-20
    郭巧生.半夏种内形态变异的模糊聚类分析[J].植物资源与环境,1997,6(3):29-34
    国家药典委员会.中华人民共和国药典[S].一部,北京:化学工业出版社,2005:78
    郝燕燕,李文来,黄卫东.高温锻炼对苹果果皮适应突发性强光能力的影响[J].园艺学报,2007,34(6):1347-1352
    何凤梨,王飞,魏钦平,等.桃树冠层相对光照分布与果实产量品质关系的研究[J].中国农业科学,2008,41(2):502-507
    何金明,肖艳辉,王羽梅,等.光照长度对茴香植株生长及精油含量和组分的影响[J].生态学报,2010,30(3):0652-0658
    何亚丽,刘友良,陈权,等.水杨酸和热锻炼诱导的高羊茅幼苗的耐热性与抗氧化的关系[J].植物生理与分子生物学报,2002,28(2):89-95.
    侯典云,崔向波,王荔.不同底肥施用量对组培半夏产量的影响[J].河南农业科学,2006,(5):94-95
    侯典云,王荔,杨艳琼.半夏不同居群的细胞学研究[J].云南农业大学学报,2005,20(2):159-162
    胡玉涛,王沫,肖平阔.半夏的生物学特性研究概况[J].湖北林业科技,2006,(6):38-41
    黄成林,吴泽民,姚永康,等.遮阴条件下绞股蓝光合作用特点的研究[J].应用生态学报,2004,15(11)∶2099-2103
    黄俊,郭世荣,蒋芳玲,等.遮阴处理及恢复光照对白菜生长及活性氧代谢的影响[J].园艺学报,2008,35(5):753-756
    黄卫东,吴兰坤,战吉宬.中国矮樱桃叶片生长和光合作用对弱光环境的适应性调节[J].中国农业科学,2004,37(12):1981-1985
    霍常富,政权,孙海龙,等.光照和氮交互作用对水曲柳幼苗生长、生物量和氮分配的影响[J].应用生态学报,2008,19(8):1658-1664
    贾士芳,董树亭,王空军,等.弱光胁迫对玉米产量及光合特性的影响[J].应用生态学报,2007,18(11):2456-2461
    贾士芳,李从锋,董树亭,等.花后不同时期遮光对玉米粒重及品质影响的细胞学研究[J].中国农业科学,2010,43(5):911-921
    蒋立昶,潘炳文,许卫东,等.半夏高产栽培新技术,山东:鲁荷新闻出版社,1999.37
    缴丽莉,路丙社,周如久,等.遮光对青榨槭光合速率及叶绿素荧光参数的影响[J].园艺学报,2007,34(1):173-178
    李霞,王洋,阎秀峰.光强对黄檗幼苗三种生物碱含量的影响[J].生态学报,2009,29(4):1655-1660
    李潮海,栾丽敏,尹飞,等.弱光胁迫对不同基因型玉米生长发育和产量的影响[J].生态学报,2005,25(4):824-830
    李晶,阎秀峰,祖元刚.2000.低温胁迫下红松幼苗活性氧的产生及保护酶的变化.植物学报,42(2):148-152.
    李名旺.半夏属若干变异式样及其演化[J].武汉植物学研究,1997,15(4):317-322
    李鹏民,高辉远.快速叶绿素荧光诱导动力学分析在光合作用研究中的应用.植物生理与分子生物学报,2005,31(6):559-566.
    李西文,陈士林.遮阴下高原濒危药用植物川贝母(Fritillaria cirrhosa)光合作用和叶绿素荧光特征[J].生态学报,2008,28(7):3438-3446
    李衍素,高俊杰,陈民生,等.2007.高温胁迫对豇豆幼苗叶片膜伤害与保护性物质的影响.山东农业大学学报,自然科学版,38(3):378-382
    李永庚,于振文,梁晓芳,等.小麦产量和品质对灌浆期不同阶段低光照强度的响应[J].植物生态学报,2005,29(5):807-81
    梁艳荣,胡晓红,张颖力,等.植物过氧化物酶生理功能研究进展.内蒙古农业大学学报,2003,24(2):110-113
    刘波,王荔,陈疏影.36份不同居群半夏同工酶研究[J].云南农业大学学报,2008,23(1):11-14
    刘婷,秦彩玲,张毅.5个不同产地半夏镇咳作用的实验研究[J].中国实验方剂学杂志,2007,13(9):35-37
    刘伯坤,李华芬.半夏的休眠观察[J].中药材,1989,12(4):9-10
    刘承训.半夏的种植管理与加工[J].四川农业科技,2008(7):40
    刘国顺,赵献章,韦凤杰,等.旺长期遮光及光照转换对不同烟草品种光合效率的影响[J].中国农业科学,2007,40(10):2368-2375
    刘启先,朱秀芳.宿半夏倒苗机理及预防技术研究[J].安徽农业科学,2003,31(4):692-693
    刘文海,高东升,束怀瑞.不同光强处理对设施桃树光合及荧光特性的影响[J].中国农业科学,2006,39(10):2069-2075
    刘奕清,陈泽雄,杨婉晴.高温和干旱胁迫对尾巨桉幼苗生理特性的影响[J].园艺学报,2008,35(5):761-764
    刘悦萍,黄卫东,张俊环.高温锻炼和水杨酸预处理对热激下葡萄叶肉细胞超微结构的影响[J].园艺学报,2006,33(3):491-495
    刘悦秋,孙向阳,王勇,等.遮阴对异株荨麻光合特性和荧光参数的影响[J].生态学报,2007,27(8):3457-3464
    罗丽兰,石雷,姜闯道,等.不同温度下新铁炮百合幼苗的光合特性及其保护机制[J].园艺学报,2008,35(1):131-136
    马德华,庞金安,霍振荣,等.黄瓜对不同温度逆境的抗性研究[J].中国农业科学,1999,32(5):28-35
    马德华,庞金安,李淑菊,等.1998.温度逆境锻炼对高温下黄瓜幼苗生理的影响.园艺学报,25(4):350-355.
    马德华,孙其信.温度逆境对不同品种黄瓜幼苗膜保护系统的影响.西北植物学报,2001,21(4):656-666.
    马开森,丁季春,钟国跃,等.不同来源地的半夏种源对比栽培试验[J].中国中药杂志,2004,29(2):184-185
    马银山,王晓芬,张作亮,等.光照强度和肥力变化对冷地早熟禾生长的影响[J].中国农业科学,2009,42(10):3475-3484
    毛炜光,吴震,黄俊,等.水分和光照对厚皮甜瓜苗期植株生理生态特性的影响[J].应用生态学报,2007,18(11):2475-2479
    毛子成,彭正松.半夏研究进展[J].江西科学,2002,20(1):42-46
    孟祥春,彭建宗,王小菁.光和糖对非洲菊花色素苷积累及CHS、DFR基因表达的影响[J].园艺学报,2007,34(1):227-230
    莫英,傅华龙,卿人韦,等.生长温度对盾叶薯蓣POD活性等生理特性的影响[J].应用与环境生物学报,2005,11(2):168-170
    潘炳文,蒋立昶,沙启营,等.夏季覆盖麦秸(糠)预防半夏倒苗实验观察[J].中国中药杂志,1996,21(9):532-533
    潘远智,江明艳.遮阴对盆栽一品红光合特性及生长的影响[J].园艺学报,2006,33(1):95-100
    彭长连,林植芳,林桂珠.光氧化胁迫下几种植物叶片的超氧自由基产生速率和光合特性.植物生理学报,2000,26(2):81-87.
    冉懋雄.名贵中药材绿色栽培技术[M].北京:科学技术文献出版社,2002:12-13
    任坚毅,林玥,岳明.太白山红桦种子的萌发特性[J].植物生态学报,2008,32(4):883-890
    沈立荣,薛小红,陈集双.半夏病毒性缩叶病鉴定初报[J].中国中药杂志,1992,17(4):209
    沈立荣,薛小红.红天蛾的初步研究[J].植物保护,1993,(5):9-10;沈立荣,薛小红.芋双线天蛾的研究[J].中药材,1991,14(6):6-8
    盛海燕,李伟成,常杰.伞形科两种植物幼苗生长对光照强度的可塑性响应[J].生态学报,2006,26(6):1854-1861
    盛海燕,李伟成,葛滢.明党参幼苗存活与生长对光照强度的响应[J].应用生态学报,2006,17(5):783-788
    四川省中医药研究院南川药物种植研究所,四川省中药材公司.四川中药材栽培技术.重庆:重庆出版社,1988.178
    宋金斌,黄裔,徐光生等.喷施亚硫酸钠液对半夏的增产效应[J].时珍国药研究,1997,3(1):94-95
    宋子健,李良霞,李建龙,等.高温胁迫对高羊茅草坪草氮代谢的影响[J].贵州农业科学,2007,35(6):15-18
    眭晓蕾,毛胜利,王立浩,等.弱光条件下辣椒幼苗叶片的气体交换和叶绿素荧光特性[J].园艺学报,2007,34(3):615-622
    眭晓蕾,张振贤,张宝玺,等.不同基因型辣椒光合及生长特性对弱光的响应[J].应用生态学报,2006,17(10):1877-1882
    唐茜,施嘉璠.川西茶区主栽品种光合强度与叶片结构相关关系的研究[J].四川农业大学学报,1997,15(2):193-198
    田良才,张明仪,李晋川.高温胁迫-小麦超高产的主要障碍.山西农业科学,1995,23(2):14-18
    汪炳良,徐敏,史庆华,等.高温胁迫对早熟花椰菜叶片抗氧化系统和叶绿素及其荧光参数的影响[J].中国农业科学,2004,37(8):1245-1250
    王利军,黄卫东,战吉成.2003.水杨酸和高温锻炼与葡萄抗热性及抗氧化的关系.园艺学报,30(4):452-454.
    王利军,李绍华,李家永,等.温度逆境交叉适应对葡萄叶片膜脂过氧化和细胞钙分布的影响[J].植物生态学报,2004,28(3):326-332
    王文琪,王进军,赵志模.紫茎泽兰种子种群动态及萌发特性[J].应用生态学报,2006,17(6):982-986
    王小伟,尚志华,张强,等.樱桃透光和郁闭树冠相对光照强度及其果实品质和产量的差异[J].园艺学报,2009,36(2):157-162
    王新胜,吴艳芳,马军营,等.半夏化学成分和药理作用研究[J].齐鲁药事,2008,27(2):101-103
    王振月,崔红花,王宗权,等.光强对毛脉酸模根中白藜芦醇和白藜芦醇苷的影响[J].应用生态学报,2006,17(5):851-854
    魏钦平,鲁韧强,张显川,等.富士苹果高干开心形光照分布与产量品质的关系研究[J].园艺学报,2004,31(3):291-296
    魏淑红,彭正松.半夏群体性状变异类型研究[J].江苏农业科学,2004,(4):37-39
    魏淑红,彭正松.半夏种内各变异类型主要形态学性状比较研究[J].现代中药研究与实践,2007,22(3):7-9
    吴彦,刘庆,何海,等.光照与温度对云杉和红桦种子萌发的影响[J].应用生态学报,2004,15(12)∶2229-2232
    吴韩英,寿森炎,朱祝军,等.高温胁迫对甜椒光合作用和叶绿素荧光的影响[J].园艺学报,2001,28(6):517-521
    吴能表,谈锋,肖文娟,等.光强因子对少花桂幼苗形态和生理指标及精油含量的影响[J].生态学报,2005,25(5):1159-1164
    伍菊仙,杨明,郭孟璧,李建成,等.不同栽培措施对大麻酚类物质含量的影响研究[J].中国麻业科学,2010,32(2):94-98
    武菊英,滕文军,袁小环,等.分株和遮阴对花叶芒生长的影响[J].园艺学报,2009,36(11):1691-1696
    肖杰易,宋廷杰.不同种植深度对半夏产量的影响[J].重庆中草药研究,1999,40:20-21
    肖培根.半夏.新编中药杂志[M].北京化学工业出版社,2002:372-378
    徐胜,何兴元,陈玮,等.高羊茅对高温的生理生态响应[J].应用生态学报,2007,18(10):2219-2226
    徐胜,何兴元,陈玮.热锻炼对高羊茅(Festuca arundinacea)和多年生黑麦草(Lolium perenne)抗高温能力的影响[J].生态学报,2008,28(1):162-171
    许良政,刘志伟,刘惠娜,等.不同光照条件对缺铁黄瓜根系Fe3+还原酶活性的影响[J].中国农业科学,2008,41(6):1865-1871
    颜坤,陈娜,曲妍妍,等.高温胁迫下转甜椒正义甘油-3-磷酸酰基转移酶基因(GPAT)烟草缓解PSⅡ光抑制[J].中国农业科学,2007,40(11):2468-2473
    杨俊霞,郭宝林,鲁韧强,等.遮阴对美国黑莓生长及光合特性的影响[J].园艺学报,2005,(2):292-294
    杨期和,葛学军,叶万辉,等.矮沙冬青种子特性和萌发影响因素的研究[J].植物生态学报,2004,28(5)651-656
    杨兴洪,邹琦,赵世杰.遮阴和全光下生长的棉花光合作用和叶绿素荧光特征[J].植物生态学报,2005,29(1):8-15
    杨兴有,刘国顺.成熟期光强对烤烟理化特性和致香成分含量的影响[J].生态学报,2007,27(8):3450-3456
    杨兴有,叶协锋,刘国顺,等.光强对烟草幼苗形态和生理指标的影响[J].应用生态学报,2007,18(11):2642-2645
    姚允聪,王绍辉,孔云.弱光条件下桃叶片结构及光合特性与叶绿体超微结构变化[J].中国农业科学,2007,40(4):855-863
    于超,张明,王宇.栽培、野生及不同产地半夏总生物碱测定[J].中国中药杂志,2004,29(6):583-584
    于超,张明,王宇.紫外分光光度法测定不同产地半夏中总生物碱的含量[J].世珍国医国药,2002,13(2):73-75
    于倩,李明军.怀山药微型块茎愈伤组织的诱导形成及高频率再生[J].生态学报,2004,24(5):1022-1026
    俞海琪,金永昌,寿伟国.半夏花奇特性及提高结实率研究[J].现代应用药学,1991,8(4):5-8
    袁燕东,王学军,李胜光等.半夏种子、珠芽与组培试管苗生长发育特性比较[J].基层中药杂志,2001,15(1):28-29
    袁野,吴凤芝,周新刚.光氮互作对番茄果实糖积累及蔗糖代谢相关酶活性的影响[J].中国农业科学,2009,42(4):1331-1338
    岳玉苓,魏钦平,张继祥,等.黄金梨棚架树体结构相对光照强度与果实品质的关系[J].园艺学报,2008,35(5):625-630
    战吉宬,黄卫东,王秀芹,等.弱光下生长的葡萄叶片蒸腾速率和气孔结构的变化[J].植物生态学报,2005,29(1):26-31
    张杰,徐涛,张建光,等.半夏栽培品遗传差异的AFLP分析[J].中草药,2008,38(12):1884-1889
    张桂莲,陈立云,张顺堂.高温胁迫对水稻花粉粒性状及花药显微结构的影响[J].生态学报,2008,28(3):1089-1097
    张国泰,郭巧生,王康才.半夏生态研究.中国中药杂志,1995,20(7):395-397
    张欢,徐志刚,崔瑾,等.光质对番茄和莴苣幼苗生长及叶绿体超微结构的影响[J].应用生态学报,2010,21(4):959-965
    张吉旺,董树亭,王空军,等.遮阴对夏玉米产量及生长发育的影响[J].应用生态学报,2006,17(4):657-662
    张建光,刘玉芳,孙建设,等.光照强度对苹果果实表面温度变化的影响[J].生态学报,2004,24(6):1306-1310
    张洁,李天来.日光温室亚高温对番茄光合作用及叶绿体超微结构的影响[J].园艺学报,2005,32(4):614-619
    张金政,周美英,李晓东,等.氮素水平与光强互作对‘蓝伞’玉簪生长和光合特性的影响[J].园艺学报,2007,34(6):1497-1502
    张君毅,郭巧生,卫新荣,等.半夏不同居群主要经济性状比较研究[J].中国中药杂志,2007,32(12):1145-1148
    张君毅,郭巧生,吴丽伟.我国不同地区半夏rDNA序列分析[J].中国中药杂志,2006,31(21):1768-1772
    张俊环,黄卫东.葡萄幼苗在温度逆境交叉适应过程中活性氧及抗氧化酶的变化[J].园艺学报,2007,34(5):1073-1080
    张科卫,吴皓,崔小兵.不同产区半夏药材中鸟苷含量的测定[J].中成药,2000,22(11):769-771
    张黎萍,荆奇,戴廷波,等.温度和光照强度对不同品质类型小麦旗叶光合特性和衰老的影响[J].应用生态学报,2008,19(2):311-316
    张黎萍,荆奇,戴廷波,等.温度和光照强度对不同品质类型小麦旗叶光合特性和衰老的影响[J].应用生态学报,2008,19(2):311-316
    张黎萍,荆奇,戴廷波,等.温度和光照强度对不同品质类型小麦旗叶光合特性和衰老的影响[J].应用生态学报,2008,19(2):311-316
    张玲玲,孙芳芳,温达志.薇甘菊和飞机草抗氧化物和膜脂过氧化物对光照和土壤水分的响应[J].植物生态学报,2009,33(5):974-983
    张明,钟国跃,马开森,等.半夏倒苗原因的实验观察研究[J].中国中药杂志,2004,4(3):273-274
    张明,钟国跃,王德立,等.半夏种植密度及底肥施用水平的研究[J].现代中药研究与实践,2003,17(4):23-24
    张乃华,高辉远,邹琦.Ca2+缓解NaCl胁迫引起的玉米光合能力下降的作用.植物生态学报,2005,29(2):324-330.
    张瑞华,徐坤,董灿兴,等.光质对姜生长及光能利用特性的影响[J].园艺学报,2008,35(5):673-680
    张苏锋.半夏人工种子的研究[J].信阳师范学院学报(自然科学版),1998,11(3):281-282
    张小斌.不同产地半夏中β-谷甾醇含量比较[J].山西农业科学,2008,(2):81-82
    张雁萍,杨志刚,洪勇.不同密度对间套作半夏经济性状和产量的影响[J].安徽农业科学,2008,36(12):5041-5043
    张银丽,杜红红,李杨,等.消毒方式、无机盐浓度及光照强度对槲蕨孢子繁殖的影响[J].园艺学报,2009,36(5):711-716
    章建新,赵明,王美云,等.小麦花药超微结构对短时高温的敏感性反应[J].华北农学报,2000,15(2):48-52
    赵辉,戴廷波,姜东,等.高温下干旱和渍水对冬小麦花后旗叶光合特性和物质转运的影响[J].应用生态学报,2007,18(2):333-338
    赵磊,杨延杰,林多.光照强度对蒲公英光合特性及品质的影响[J].园艺学报,2007,34(6):1555-1558
    赵丽英,邓西平,山仑.活性氧清除系统对干旱胁迫的响应机制.西北植物学报,2005,25(2):413-418
    赵瑞芬,焦晓燕,杨治平,等.光照对绿豆扦插苗不定根生长硼需求的影响[J].应用生态学报,2006,17(5):801-804
    周忆堂,马红群,梁丽娇,等.不同光照条件下长春花的光合作用和叶绿素荧光动力学特征[J].中国农业科学,2008,41(11):3589-3595
    Baek K H, Skinner D Z.2003. Alteration of antioxidant enzyme gene expressionduring cold acclimation of near_isogenic wheat lines. Plant Science:1221-1227.
    Berry J,Bjorkman O.1980. Photosynthetic response and adaptation to temperature inhigher plants. Ann Rev Plant Physiology,31:491-543.
    Camejo D,Rodriguez P,Morales M A,Dell’Amico J M,Torrecillas A,Alarcón JJ.2005.High temperature effects on photosynthetic activity of two tomato cultivarswith different heat susceptibility. J plant Physiol,162(3):281-289.
    Chen G-X (陈国祥), Zhang R-X (张荣铣). Study on characteristics of PS Ⅱof flagleaves during photosyn-thetic functional decline process ofYangmai5(Triticumaestivum L.).Scientia Agricultura Sinica(中国农业科学),2004,37(1):36-42(inChinese)
    Chen T H H,Shen Z Y,Lee P H.1982. Adaptability of crop plants to hightemperature stress. Crop Science,22:719-725.
    Demmig B, Winter K, Kruger A. Czygan Franz-Christian. Photoinhibitionandzeaxanthin formation in intact leaves: a possible role of the xanthophyllcyclein the dissipation of excess light energy. Plant Physiology,1987,84:218-222
    Demmig-Adams B,Adams ⅢW W.1996. Xanthophyll Cycle and light stress innature: uniform response to excess direct sunlight among higher plant specie.Planta,198(3):460-470.
    Dirk I,Marc V M. Oxidative stress in plants. Current0pinion in Biotechnology,1995,6:153-158.
    Dubay R, Pessarakli M. Physiological mechanisms of nitrogen absorption andassimilation in plants under stressful conditions [M]. New York: MarcelDekker,1995,605-626
    Foyer C H, Descourvieres P, Kunert K J.1994. Protection against oxygen radical animportant defense mechanism studied in transgenic plants. Plant Cell Environ,17:507-707
    Fu P,Wilen R W,Roertson A J,Low N H,Tyler R T,Gusta L V.1998. Heat toleranceof cold acclimated puma winter rye seedlings and the effect of a heat shock onfreezing tolerance. Plant and Cell physiology,39:942-949.
    Glena A, Gilbert M V, Gadush C W, et al. Amino acid accumulation in sink andsource tissues of coleus blumei benth during salinity stress[J]. ExpBot,1998,49:107-114.
    Good A, Zaplachiniski S, The effects of drought stress on free amino acidaccumulation and protein synthesis in Brassicanapus[J]. PhysiolPlant,1994,90:9-14.
    Gray G R,Chauvin L P,Sarhan F,Huner N P A.1997. Cold acclimation and freezingtolerance-acomplex interaction of light and temperature. Plant Physiology,114:467-474.
    Hong S S, Xu D Q. Light-induced increase in initial fluorescence parameters to stronglight between wheat and soybean leaves. Chinese Science Bulletin,1997,42:684-688
    Jenner C F,Siwek K,Hawker J S. The synthesis of14C starchfrom14C sucroce inisolated wheat grains is dependent upon the activity of soluble starch synthase.Australian Joumal of Plant Physiology,1993,20:329-335
    Keeling P L, Bacon P J,Holt D c. Elevated temperature reduced starch deposition inwheat endosperm by reducing the activity of soluble starch synthase. Planta,1993,191:342-348
    Kratsch H A,Wise R R.2000. The ultrastructure of chilling stress. Plant,Cell andEnvironment,23:337-350.
    Lee D H, Lee C B.2000. Chilling stress-induced changes of antioxidant enzymes inthe leaves of cucumber: in gel enzyme activity assays. Plant Science,159:75-85.
    Llorens N, Arola L, Blade C, et al. Effects of copper exposure upon nitrogenmetabolism in tissue cultured Vitis vinifera[J]. Plant Sci,2000,160:159-163
    Maevskaya S, Egorova E A., Bukhov N G. Effect of elevated temperature on nitriteand nitrate reduction in leaves and intact chloroplasts Russ[J]. Plant Physiol,2003,50:675-679
    Makino A, Nakano H, Mae T. Effects of growth temperature on the responses ofribulose-1.5-bisphate carboxylase, electron transport components, and sucrosesynthesis enzymes to leaf nitrogen in rice, and their relationships tophotosynthesis[J]. Plant Physiol,1994,105:1231-1238
    Monneveux P, Pastenes C, Reynolds M P. Limitations to photosynthesis under lightand heat stress in three high-yielding wheat genotypes. Journal of PlantPhysiology,2003,160(6):657-666
    Okuda T,Matsuda Y,Yamanaka A,Sagisaka S.1991.Abrupt increase in the level ofhydrogen-peroxide in leaves of winter wheat is caused by cold treatment. PlantPhysiology,97:1265-1267.
    Olugbemi L B,Bush M G. The influence of temperature on the contribution of awnsto yield in wheat.Australian Joumal of Plant Physiology.1987,14:299-310
    Poovaiah, B.W.&A.S.N. Reddy.1993. Calicium and signal transduction in plants.Critical Review of Plant Science,12:185-221.
    Puhakainen T, Pihakaski-Maunsbach K, Widell S, Sommarin M.1999.Coldacclimation enhances the activity of plasma membrane Ca2+ATPase in winter ryeleaves. Plant Physiology and Biochemistry,37(3):231-239.
    Rijven A H G C. Heat inactivation of starch synthase in wheat endosperm tissue. PlantPhysiology.1986,81:448-53
    Schubert S, Serraj R, Plies-Balzer E, et al. Effect of drought stress on growth, sugarconcentrations and amino acid accumulation in N2fixing alfalfa[J]. PlantPhysiol,1995,46:541-546.
    Schwalbe M, Teller S, Oelmüller, et al. Influence of UVB irradiation on nitrate andammonium assimilating enzymes in Spirodela polyrhiza[J]. AquatBot,1999,64:19-34
    Tewan A K,TriPathy B C. Temperature-stress-induced impaired of chlorophyllbiosynthetic reactions in cucumber and wheat. Plant Physiology.1998,117:851-858.
    Thebud R, Santarius K A. Effects of high-temperature stress on variousbiomembranes of leaf cells in Situ and in Vivo. Plant Physiology,1987,70:200-205.
    Triboi E, Martre P, Triboi-BlondelAM. Environmentally-induced changes in proteincomposition in developing grains of wheat are related to changes in total proteincontent. Journal of Experimental Botany,2003,54(388):1731-1742
    Vani B,Saradhi P P,Mohanty P.2001. Alteration in chloroplast structure andthylakoid membrane composition due to in vivo heat treatment of rice seedlings:correlation with the functional changes. Journal of Plant physiology,158:583-592.
    Willekens H, Camp W V, Montagu M V, inzé D. Sulfur dioxide and ultraviolet-B havesimilar effects on mRNA accumulation of antioxidant genes in NicotianaPlumbaginifolia L. Plant physiology,1994,106:1007-1014.
    Yang XH, Chen XY, Ge QY, et al. Tolerance of photosynthesis to photoinhibition,high temperature and drought stress in flag leaves of wheat: A comparisonbetween a hybridization line and its parents grown under field conditions. PlantScience,2006,171(3):389-397
    Youngner V B, Nudge F G. Growth and carbohydrate storage of three Poa pratensis L.strains as influenced by temperatures. Crop Sci.,1968,8:455-456
    Zhang J H,Huang W D,Liu Y P,Pan Q H.2005. Effect of temperature acclimationpretreatment on the ultrastructure of mesophyll cell in young grape plants undercross temperature-stresses. Journal of Integrative Plant Biology,47:959-970.
    Zhang J H,Liu Y p,Pan Q H,Zhan J C,Wang X Q,Huang W D.2006. Changesin membrane-associated H+-ATpase activities and amounts in young grape plantsduring the cross adaptation to temperature stresses. Plant Science.170:768-777.

© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号

地址:北京市海淀区学院路29号 邮编:100083

电话:办公室:(+86 10)66554848;文献借阅、咨询服务、科技查新:66554700