小麦生理型雄性不育能量代谢途径中TaPDK基因的表达及其互作蛋白的研究
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摘要
化学杂交剂诱导的生理型不育(PHYMS)是小麦雄性不育系的重要来源之一。SQ-1是一种新型化学杀雄剂,它能诱导小麦产生95%-100%的雄性不育。在生产上,应用SQ-1已经组配出一批超高产、优质、多抗的杂交小麦新品种,但是对于PHYMS的机理尚不清楚,尤其是对PHYMS花粉败育的内部代谢知之甚少。为了阐明PHYMS花药发育能量代谢过程的作用机制,本研究首先通过细胞学的方法对PHYMS系的花药绒毡层发育,小孢子的细胞分裂及淀粉积累等情况进行了观察,同时测定了丙酮酸含量,初步分析了能量供应对小麦花粉发育的影响；并以[aPDK作为研究的主体,采用普通克隆技术,TAIL-PCR以及原核表达对该基因的分子特征进行了分析；其次采用半定量RT-PCR技术检测了TaPDK及其底物蛋白基因TaPDC-E1α在小麦花药中的表达；最后,利用酵母双杂交技术筛选了TaPDK的互作蛋白,并结合实时定量PCR技术分析了这些蛋白基因的表达模式,进一步探讨了这些基因与能量代谢以及花粉发育的关系。获得的主要结果及结论如下：
1.细胞学观察结果表明,与正常可育系相比,绒毡层在PHYMS系的小孢子发育早期提前解体；小孢子细胞分裂出现异常,大部分小孢子细胞停止在单核和二核期不再分裂。此外,PHYMS系的成熟花粉中几乎没有淀粉颗粒的累积。丙酮酸含量的测定显示,小麦PHYMS系中的丙酮酸含量一直处于较低的运行状态。因此,致使小孢子这种异常的发育与能量供应不足密切相关。
2.小麦TaPDK基因编码364个氨基酸残基,蛋白分子量为41KD,与原核表达出的融合蛋白分子量基本一致。保守结构的分析发现TaPDK含有线粒体支链α-酮酸脱氢酶激酶家族和类组氨酸ATP酶家族；同时,进一步对其启动子序列分析表明TaPDK含有多种顺式作用元件。因此,TaPDK分子序列的功能多样性,暗示了PaPDK参与了多通路的代谢调节。
3.半定量RT-PCR结果表明,TaPDC-E1α在PHYMS系中表达量最低,说明经杀雄剂SQ-1诱导形成的生理型不育系在败育过程中其能量代谢途径更容易受到影响。此外,TaPDK基因在PHYMS系中表达下调,而在遗传型不育系中表达上调,表明对TaPDK基因进行调节的上游信号机制在小麦PHYMS系与遗传型不育系中不一致。
4.通过酵母双杂交技术筛选TaPDK的互作蛋白,共获得24个阳性克隆,这些基因共编码8种不同的蛋白质,分别是多聚泛素,CBS结构域包含蛋白,非特异性脂质转移蛋白前体,增殖细胞核抗原,甘油醛-3-磷酸脱氢酶,肌动蛋白,鸟嘌呤核苷酸结合蛋白p亚基,查尔酮合成酶基因及功能未知蛋白。这些候选蛋白参与了蛋白降解,能量供应,细胞分裂及花粉的形成,这表明TaPDK除参与调节丙酮酸脱氢酶复合体的活性外,该蛋白在花粉发育的过程中还起着其他多样化的作用。
5.对TaPDK互作蛋白基因的定量表达分析表明,TaPUbi仅在PHYMS系中表达上调,在同遗传背景的遗传型不育系和相应的近等基因系中表达没有变化。因此,说明SQ-1的喷施诱导了该基因的表达,多聚泛素介导的泛素蛋白酶体途径在PHYMS系中参与了对TCA途径的能量调节。TaPCNA在不育系和可育系中的表达一直呈现出依次下降的表达趋势,但是无论是在PHYMS系还是同背景的遗传型不育系中,TaPCNA的表达均高于可育系。TaCBS在不育系及可育系中无明显差异,但在三核期,该基因在不育系中的表达高于可育系。这可能是不育系中花粉发育后期能量严重不足,CBS结构域蛋白作为细胞能量的传感器,在SQ-1诱导花粉败育的能量应急中参与能量水平的调节。TanLTP在不育系中的高表达,应该与SQ-1喷施后花粉形成过程的防御反应有关。
Currently, the physiological male sterility (PHYMS) induced by chemical hybridizing agent (CHA) is an important source of male sterile line in wheat (Triticum aestivum L.). SQ-1, a new chemical hybridizing agent, can induce95%-100%spikelet sterility in wheat. In practice, a series of new hybrid wheat cultivars with high yield, elite quality, and multiple resistance were developed by making use of SQ-1. However, very little is known about the molecular mechanism of PHYMS. The challenge remains to understand how intermediary metabolism can be regulated during pollen abortion. To elucidate energy metabolism mechanism of the abnormal pollen development in PHYMS, the cytological observation of tapetum development, cell division, starch accumulation in PHYMS were performed, and the content of pyruvic acid was detected. Furthermore, the molecular characteristics of TaPDK, as the research starting point, were analyzed via common clone, hiTAIL-PCR, and prokaryotic expression. Then the expression pattern of TaPDK and its substrate protein gene TaPDC-E1α were detected via semiquantitative RT-PCR analyses. In addition, the proteins interacting with TaPDK were screened via yeast two-hybrid technique. Subsequently, a few candidate proteins in nucleotide expression levels were detected by real-time quantitative PCR, and further discussed the relationship between these genes expression and pollen development.The main results and conclusions were as follows:
1. Cytological observations showed that tapetum was premature degeneration at early microspore stage in PHYMS, and the majority of microspores failed to divide and remained bicellular status. Additionally, there was no starch accumulation in the mature pollen of PHYMS. Subsequently, the results from the pyruvic acid detection showed that the content of pyruvic acid was dramatically down-regulated in the PHYMS lines than that of the fertile lines. Collectively, the abnormal microspore development was associated with energy shortage.
2. The TaPDK encoded364amino acid residues, and protein molecular weight was41KD. The protein molecular weight of TaPDK from prokaryotic expression was consistent with software prediction. Conservative structure analysis found that TaPDK contains BCDHK-Adom3superfamily and HATPase-c superfamily. Subsequently, the upstream regulation sequences of TaPDK contained multiple cis-elements. Therefore, the structure diversity of TaPDK molecular sequence suggested that the TaPDK may be involved in the metabolic regulation of multiple pathway.
3. According to semiquantitative RT-PCR analyses, the expression levels of TaPDC-E1α was lowest in PHYMS, demonstrating that the energy metabolism pathway was more susceptible in the PHYMS. TaPDK was obviously down-regulated expression in PHYMS, and up-regulated expression in genetic male sterile lines, which indicated that the upstream signal mechanism of mediating TaPDK was inconsistent between the PHYMS and the genetic male-sterile lines.
4. We obtained twenty-four colonies coding for eight different proteins via yeast two-hybrid screen, namely non specific lipid transfer protein precursor, polyubiquitin, glyceraldehyde-3-phosphate dehydrogenase, proliferating cell nuclear antigen, CBS domain containing protein, actin, guanine nucleotide-binding protein beta subunit, chalcone synthase, and unknown function protein. These proteins involved in protein degradation, energy supply, cell division, and pollen formation, which further suggested that TaPDK plays multiple roles in pollen development, besides participating in regulating pyruvate dehydrogenase complex activity.
5. The quantitative RT-PCR analysis indicated that the expression level of TaPUbi was only obviously up-regulated in PHYMS lines, but no significant difference in TaPUbi transcripts was observed in the corresponding genetic male sterility and fertility. It suggested that the SQ-1spraying induced TaPUbi expression, and ubiquitin proteasome pathway may participate in regulating the energy status of TCA pathway in PHYMS. The TaPCNA expression in sterile and fertile lines gradually exhibited down-regulated pattern with the pollen developmemt, but its expression was still higher in PHYMS lines and genetic male sterile lines than in fertile lines. Whereas the expression of TaCBS maintained stable, no significant differences were detected in leaf and anther at the uninucleate and binucleate stage between PHYMS and fertile lines. Intriguingly, TaCBS expression was much more increased at the tricellular stage in PHYMS lines than in fertile lines. It was speculated that the energy supply was serious shortage in later pollen development of PHYMS lines, and TaCBS, as cell energy sensor, adjusted energy levels to deal with energy crisis during pollen abortion in PHYMS lines. The expression of TanLTP was higher in PHYMS lines than in fertile lines. This indicated that the highest expression of TanLTP might protect pollen against potential disruption after SQ-1treatment.
1.细胞学观察结果表明,与正常可育系相比,绒毡层在PHYMS系的小孢子发育早期提前解体；小孢子细胞分裂出现异常,大部分小孢子细胞停止在单核和二核期不再分裂。此外,PHYMS系的成熟花粉中几乎没有淀粉颗粒的累积。丙酮酸含量的测定显示,小麦PHYMS系中的丙酮酸含量一直处于较低的运行状态。因此,致使小孢子这种异常的发育与能量供应不足密切相关。
2.小麦TaPDK基因编码364个氨基酸残基,蛋白分子量为41KD,与原核表达出的融合蛋白分子量基本一致。保守结构的分析发现TaPDK含有线粒体支链α-酮酸脱氢酶激酶家族和类组氨酸ATP酶家族；同时,进一步对其启动子序列分析表明TaPDK含有多种顺式作用元件。因此,TaPDK分子序列的功能多样性,暗示了PaPDK参与了多通路的代谢调节。
3.半定量RT-PCR结果表明,TaPDC-E1α在PHYMS系中表达量最低,说明经杀雄剂SQ-1诱导形成的生理型不育系在败育过程中其能量代谢途径更容易受到影响。此外,TaPDK基因在PHYMS系中表达下调,而在遗传型不育系中表达上调,表明对TaPDK基因进行调节的上游信号机制在小麦PHYMS系与遗传型不育系中不一致。
4.通过酵母双杂交技术筛选TaPDK的互作蛋白,共获得24个阳性克隆,这些基因共编码8种不同的蛋白质,分别是多聚泛素,CBS结构域包含蛋白,非特异性脂质转移蛋白前体,增殖细胞核抗原,甘油醛-3-磷酸脱氢酶,肌动蛋白,鸟嘌呤核苷酸结合蛋白p亚基,查尔酮合成酶基因及功能未知蛋白。这些候选蛋白参与了蛋白降解,能量供应,细胞分裂及花粉的形成,这表明TaPDK除参与调节丙酮酸脱氢酶复合体的活性外,该蛋白在花粉发育的过程中还起着其他多样化的作用。
5.对TaPDK互作蛋白基因的定量表达分析表明,TaPUbi仅在PHYMS系中表达上调,在同遗传背景的遗传型不育系和相应的近等基因系中表达没有变化。因此,说明SQ-1的喷施诱导了该基因的表达,多聚泛素介导的泛素蛋白酶体途径在PHYMS系中参与了对TCA途径的能量调节。TaPCNA在不育系和可育系中的表达一直呈现出依次下降的表达趋势,但是无论是在PHYMS系还是同背景的遗传型不育系中,TaPCNA的表达均高于可育系。TaCBS在不育系及可育系中无明显差异,但在三核期,该基因在不育系中的表达高于可育系。这可能是不育系中花粉发育后期能量严重不足,CBS结构域蛋白作为细胞能量的传感器,在SQ-1诱导花粉败育的能量应急中参与能量水平的调节。TanLTP在不育系中的高表达,应该与SQ-1喷施后花粉形成过程的防御反应有关。
Currently, the physiological male sterility (PHYMS) induced by chemical hybridizing agent (CHA) is an important source of male sterile line in wheat (Triticum aestivum L.). SQ-1, a new chemical hybridizing agent, can induce95%-100%spikelet sterility in wheat. In practice, a series of new hybrid wheat cultivars with high yield, elite quality, and multiple resistance were developed by making use of SQ-1. However, very little is known about the molecular mechanism of PHYMS. The challenge remains to understand how intermediary metabolism can be regulated during pollen abortion. To elucidate energy metabolism mechanism of the abnormal pollen development in PHYMS, the cytological observation of tapetum development, cell division, starch accumulation in PHYMS were performed, and the content of pyruvic acid was detected. Furthermore, the molecular characteristics of TaPDK, as the research starting point, were analyzed via common clone, hiTAIL-PCR, and prokaryotic expression. Then the expression pattern of TaPDK and its substrate protein gene TaPDC-E1α were detected via semiquantitative RT-PCR analyses. In addition, the proteins interacting with TaPDK were screened via yeast two-hybrid technique. Subsequently, a few candidate proteins in nucleotide expression levels were detected by real-time quantitative PCR, and further discussed the relationship between these genes expression and pollen development.The main results and conclusions were as follows:
1. Cytological observations showed that tapetum was premature degeneration at early microspore stage in PHYMS, and the majority of microspores failed to divide and remained bicellular status. Additionally, there was no starch accumulation in the mature pollen of PHYMS. Subsequently, the results from the pyruvic acid detection showed that the content of pyruvic acid was dramatically down-regulated in the PHYMS lines than that of the fertile lines. Collectively, the abnormal microspore development was associated with energy shortage.
2. The TaPDK encoded364amino acid residues, and protein molecular weight was41KD. The protein molecular weight of TaPDK from prokaryotic expression was consistent with software prediction. Conservative structure analysis found that TaPDK contains BCDHK-Adom3superfamily and HATPase-c superfamily. Subsequently, the upstream regulation sequences of TaPDK contained multiple cis-elements. Therefore, the structure diversity of TaPDK molecular sequence suggested that the TaPDK may be involved in the metabolic regulation of multiple pathway.
3. According to semiquantitative RT-PCR analyses, the expression levels of TaPDC-E1α was lowest in PHYMS, demonstrating that the energy metabolism pathway was more susceptible in the PHYMS. TaPDK was obviously down-regulated expression in PHYMS, and up-regulated expression in genetic male sterile lines, which indicated that the upstream signal mechanism of mediating TaPDK was inconsistent between the PHYMS and the genetic male-sterile lines.
4. We obtained twenty-four colonies coding for eight different proteins via yeast two-hybrid screen, namely non specific lipid transfer protein precursor, polyubiquitin, glyceraldehyde-3-phosphate dehydrogenase, proliferating cell nuclear antigen, CBS domain containing protein, actin, guanine nucleotide-binding protein beta subunit, chalcone synthase, and unknown function protein. These proteins involved in protein degradation, energy supply, cell division, and pollen formation, which further suggested that TaPDK plays multiple roles in pollen development, besides participating in regulating pyruvate dehydrogenase complex activity.
5. The quantitative RT-PCR analysis indicated that the expression level of TaPUbi was only obviously up-regulated in PHYMS lines, but no significant difference in TaPUbi transcripts was observed in the corresponding genetic male sterility and fertility. It suggested that the SQ-1spraying induced TaPUbi expression, and ubiquitin proteasome pathway may participate in regulating the energy status of TCA pathway in PHYMS. The TaPCNA expression in sterile and fertile lines gradually exhibited down-regulated pattern with the pollen developmemt, but its expression was still higher in PHYMS lines and genetic male sterile lines than in fertile lines. Whereas the expression of TaCBS maintained stable, no significant differences were detected in leaf and anther at the uninucleate and binucleate stage between PHYMS and fertile lines. Intriguingly, TaCBS expression was much more increased at the tricellular stage in PHYMS lines than in fertile lines. It was speculated that the energy supply was serious shortage in later pollen development of PHYMS lines, and TaCBS, as cell energy sensor, adjusted energy levels to deal with energy crisis during pollen abortion in PHYMS lines. The expression of TanLTP was higher in PHYMS lines than in fertile lines. This indicated that the highest expression of TanLTP might protect pollen against potential disruption after SQ-1treatment.
引文
陈季武,郑丽娜,王帮正,李晓涛.2012.泛素化介导的非蛋白质降解功能.生物化学与生物物理进展,39(7)：613-621
陈蕊红,叶景秀,张改生,王书平,李莉,牛娜,朱建楚.2009.小麦质核互作型雄性不育系及其保持系花药蛋白质组分研究与分析.生物化学与生物物理进展,36(4)：431-440
程春生,陆英飞,张改生,张宗俭,李鹏,张宝砚.2004.杀雄嗪酸的合成及其生物活性的
研究.农药,43(7)：315-317
崔秀辉.2008.化学杂交剂SQ-1诱导糜子雄性不育效果研究.作物学报,34(1)：106-110
韩艳芬,张龙雨,胡俊敏,张改生,李亚鑫,盛英,位芳,牛娜,马守才.2010.黏类小麦细胞质雄性不育线粒体atp6基因转录本编辑位点.作物学报,36(12)：2179-2184
侯国裕,黄荣初,王兴凤,杨师柏.1981.新化学杀雄剂KRIS-1的合成.农药,5：32-33
黄纯农,徐敏源,庄元忠.1981.乙烯利诱导的雄性不育小麦花粉发育的电子显微镜研究.植物学报(英文版),1：1-19
菅记涌,张勇,朱大海.2008.丙酮酸脱氢酶激酶的研究进展.基础医学与临床,28(11)：1212-1215
蒋明亮,陈万义.1995.哒嗪类植物化学杂交剂的研究概况.农药译丛,17(3)：42-44
类延宝,宋松泉,傅家瑞,程红焱.2004.植物的双组分信号系统.植物学通报,21(2)：216-227
李莉,王书平,张改生,王亮明,宋瑜龙,张龙雨,牛娜,马守才.2011.小麦生理型和遗传型雄性不育系及其保持系小花完整叶绿体蛋白质组分比较研究.作物学报,37(7)：1134-1143
李小琴,万邦惠,刘纪麟,郑用琏,李建生,徐尚忠.2004.玉米新不育胞质WBMs小孢子败育的细胞学观察.中国农业科学,37(9)：1261-1264
李亚鑫,盛英,张改生,宋瑜龙,王亮明,张龙雨,王书平,李莉.2011.杀雄剂SQ-1诱导的小麦生理型雄性不育系交替氧化酶基因(AOX1)的表达分析.麦类作物学报,31(5)：805-810
刘宏伟,张改生,刘秉华.2004.化学杂交剂GENESIS诱导小麦雄性不育的细胞形态学观察.西北植物学报,24(12)：2282-2285
刘宏伟,张改生,王军卫,王小利,方正武.2003.化学杂交剂SQ-1诱导小麦雄性不育及与不同小麦品种互作效应的研究.西北农林科技大学学报(自然科学版),31(4)：15-18
刘永生,张改生,牛娜.2007.化学杂交剂SQ-1诱导雄性不育小麦开颖特性的研究.麦类作物学报,27(3)：442-444
裴阿卫.1996.化学杂交剂WL84811诱导普通小麦雄性不育时花粉的细胞形态学观察.西北农业学报,5(3)：93-94
彭丹妮,黄静,吴自荣.2007.酵母三杂交系统的原理和应用.生命科学,19(4)：461-464
漆正堂,丁树哲,贺杰.2008.丙酮酸脱氢酶活性调控在线粒体代谢与生长平衡中的作用.生命的化学,28(3)：307-310
沈银柱,刘植义,黄占景,何聪芬,柏峰,马闻师,朱正歌,葛荣朝,许顺芝,岳聚兴,张中良.1999.不同化学杂交剂(CHA)对小麦花药同工酶影响的研究.遗传,21(5)：41-46
苏俊英,吕德彬,程西永,许海霞.2000.化学杂交剂Genesis及Sc2053对小麦穗部过氧化物酶活性的影响.河南农业大学学报,34(4)：309-311
谭才邓.2007.植物丙酮酸脱氢酶系及其调节酶的研究进展.广西轻工业,6：7-9
田爱梅,曹家树.2008.植物脂质转移蛋白.细胞生物学杂志,30：483-488
王涛,贺红武,袁均林.2003.丙酮酸脱氢酶系：除草剂品种的新靶标.应用化学,20(7)：613-617
王俊生,袁蕾,张明珠,张改生,牛娜,马守才,李红霞.2010.化学杂交剂SQ-1诱导小麦泛素/26S蛋白酶体途径的表达.农业生物技术学报,18(4)：695-701
王俊生,张改生,原蕾,张明珠,牛娜,马守才,叶景秀.2009a.化学杂交剂诱导的小麦生理型雄性不育花药的活性氧代谢.西北植物学报,29(7)：1351-1357
王俊生.2009b.小麦生理型雄性不育花药活性氧代谢和基因表达分析.[博士学位论文].杨凌：西北农林科技大学
位明明,王俊生,张改生,张龙雨,袁正杰,孙瑞,叶景秀,牛娜,马守才,李红霞.2009.GAPDH基因表达与小麦生理型雄性不育花药败育的关系。分子植物育种,7(4)：679-684
王书平,张改生,叶景秀,李莉,宋瑜龙,王亮明,代军军,张龙雨,牛娜,马守才,朱建楚.2010.杀雄剂SQ-1诱导小麦生理型雄性不育小花完整叶绿体差异蛋白质的鉴定.中国生物化学与分子生物学报,26(9)：854-861
王书文,赵寅槐,王艳平,张爱民,王道全,陈万义,黄铁城,王茂江.2000.化学杂交剂BAU9403诱导小麦雄性不育研究初报.江苏农业科学,6：18-20
王永军,张改生,王军卫,牛娜.2005.小麦遗传型与生理型雄性不育花药同工酶的比较研究.麦类作物学报,25(4)：44-49
王振华,刘宏伟,张改生,王军卫,马守才.2003.3种新型化学杂交剂诱导小麦雄性不育效果比较.西北农林科技大学学报(自然科学版),31(3)：43-46
肖建国,蒋爱湘,冯贵苓,石斯发,徐昕,冯刚,周之杭,梁咏东,尤文玲.1996.化学杂交剂“津奥啉”诱导小麦雄性不育机理研究.华北农学报,11(4)：7-11
闫先喜,梁作勤,田纪春.1996.Sc2053诱导小麦雄性不育形态学和细胞学观察.华北农学报,1(1)：19-24
杨涛.2004.化学杂交剂诱导的小麦雄性不育育性相关基因片段的分离与克隆.[中国农业大学].北京：硕士学位论文
姚雅琴,杨天章.1996.K型小麦雄性不育系花药壁及小孢子发育特征.电子显微学报,15(5)：402
叶景秀,陈蕊红,张改生,王俊生,王书平,李莉,牛娜,马守才,李红霞,朱建楚.2009a.杀雄剂SQ-1诱导小麦雄性不育花药蛋白质组分分析.农业生物技术学报,17(5)：858-864
叶景秀,张改生,王书平,陈蕊红,王俊生,牛娜,马守才,李红霞,朱建楚.2009b.杀雄剂SQ-1诱导小麦雄性不育花粉粒差异蛋白质组学研究.中国生物化学与分子生物学报,25(10)：949-957
张龙雨,袁蕾,杨书玲,张改生,王俊生,宋瑜龙,赵卓军,牛娜,马守才.2011.小麦雄性不育系中TaPDC-E1α及其调节酶基因的表达特征.作物学报,37(4)：620-628
张明珠,袁蕾,王俊生,张改生,郭文江,张龙雨.2010.顺乌头酸酶基因在杀雄剂SQ-1诱导小麦雄性不育花药中的表达分析.中国生物化学与分子生物学报,26(8)：740-748
周江菊,陈良碧,龙跃生.2001.双低两用温敏核不育水稻低温条件下花粉发育的细胞学观察.生命科学研究,5(2)：160-163
朱树勇,迟伟,张立新.2006.分裂泛素化酵母双杂交系统研究光合膜蛋白相互作用.生物化学与生物物理进展,33(4)：350-356
Araki M, Nozaki Y, Motojima K.2007. Transcriptional regulation of metabolic switching PDK4 gene under various physiological conditions. Yakugaku Zasshi,127:153-162
Ariizumi T, M Amagai, D Shibata, K Hatakeyama, M Watanabe, K Toriyama.2002. Comparative study of promoter activity of three anther-specific genes encoding lipid transfer protein, xyloglucan endotransglucosylase/hydrolase and polygalacturonase in transgenic Arabidopsis thaliana. Plant Cell Report,21:90-96.
Bateman A.1997. The structure of a domain common to archaebacteria and the homocystinuria disease protein. Trends in Biochemical Sciences,22:12-13
Baxi M D, Vishwanatha J K.1995. Uracil DNA-glycosylase/glyceraldehyde-3-phosphate dehydrogenase is an Ap4A binding protein. Biochemistry,34:9700-9707
Binet N N, Wei J H, Tessier L H.1991. Structure and expression of sunflower ubiquitin genes. Plant Molecular Biology,17:395-470
Boavida L C, Becker J D, Feijo J A.2005. The making of gametes in higher plants. Int J Dev Biol.49: 595-614
Borg M, Brownfield L, Twell D.2009. Male gametophyte development:a molecular perspective. Journal of Experimental Botany,60(5):1465-1478
Bourret R B, Borkovich K A, Simon M I.1991. Signal transduction pathways involving protein phosphorylation in prokaryotes. Annu Rev Biochem,60:401-441
Boutrot F, Meynard D, Guiderdoni E, Joudrier P, Gautier M F.2007. The Triticum aestivum non-specific lipid transfer protein (TaLtp) gene family:comparative promoter activity of six TaLtp genes in transgenic rice. Planta,225:843-862
Bravo R, Fey S J, Bellatin J, Larsen P M, Celis J E.1982. Identification of nuclear polypeptide ('cyclin') whose relative proportion is sensitive to changes in the rate of cell proliferation and to transformation. Progress in Clinical and Biological Research,85:235-248
Bryksin A V, Laktionov P P.2008. Role of glyceraldehyde-3-phosphate dehydrogenase in vesicular transport from golgi apparatus to endoplasmic reticulum. Biochemistry,73:619-625
Budde R J A, Fang T K, Randall D D.1988. Regulation of the phosphorylation of mitochondrial pyruvate dehydrogenase complex in situ. Plant Physiology,88:1031-1036
Budde R J A, Randall D D.1990. Pea leaf mitochondrial pyruvate dehydrogenase complex is inactivated in vivo in a light-dependent manner. Proceedings of the National Academy of Sciences,8:673-676
Budde R J, Fang T K, Randall D D, Miernyk J A.1991. Acetyl-Coenzyme A can regulate activity of the mitochondrial pyruvate dehydrogenase complex in situ. Plant Physiol,95:131-136
Camp P J, Miernyk J A, Randall D D.1988. Some kinetic and regulatory properties of the pea chloroplast pyruvate dehydrogenase complex. Biochim Biophys Acta,933:269-275
Camp P J, Randall D D.1985. Purification and characterization of the pea chloroplast pyruvate dehydrogenase complex:a source of acetyl-CoA and NADH for fatty acid biosynthesis. Plant Physiol, 77:571-577
Caplan J L, Mamillapalli P, Burch-Smith T M, Czymmek K, Dinesh-Kumar S P.2008. Chloroplastic protein NRIP1 mediates innate immune receptor recognition of a viral effector. Cell,132:449-462
Chakraborty K, Devakumar C, Tomar S M S, Kumar R.2003. Synthesis and quantitative structure-activity relationships of oxanilates as chemical hybridizing agents for wheat(Triticum aestivum L). Journal of agriculture and food chemistry,51(4):992-998
Chakraborty K, Devakumar C.2006a. Ethyloxanilates as specific male gametocides for wheat (Triticum aestivum L.). Plant Breeding,125:441-447
Chakraborty K, Devakumar C.2006b. Synthesis and screening of 5-carbethoxy-N-aryl-4,6-dimethyl-1,2-dihydrophrid-2-ones as chemical hybridizing agents for wheat (Triticum aestivum L.). Indial Journal of Chemistry,45B:703-714
Chakraborty K, Devakumar C.2005. N-acylanilines, herbicide-CHA chimera, and amino acid analogues as novel chemical hybridizing agents for wheat (Triticum aestivum L.). Journal of agriculture and food chemistry,53(20):7899-7907
Chakraborty K, Devakumar C.2006c. Evaluation of chemical compounds for induction of male sterility in wheat (Triticum aestivum L.). Euphytica,147:329-335
Chang L, Ma H, Xue H W.2009. Functional conservation of the meiotic genes SDS and RCK in male meiosis in the momocot rice. Cell Res,19(6):768-782
Christensen A H, Sharrok R A, Quail P H.1992. Maize polyubiquitin genes:structure, thermal perturbation of expression and transcript splicing, and promoter activity following transfer to protoplasts by electroporation. Plant Molecular Biology,18:675-689
Citovsky V, Gafni Y, Tzfira T.2008. Localizing protein-protein interactions by bimolecular fluorescence complementation in planta. Methods,45(3):196-206
Collantes E R, Xing L, Miller P C, Welsh W J, Profeta S J.1999. Comparative molecular field analysis as a tool to evaluate mode of action of chemical hybridization agents. Journal of agriculture and food chemistry,47(12):5245-5251
Currier H B, Day B E, Crafts A S.1951. Some effects of maleic hydrazide on plants. Botanical Gazette, 112(3):271-280
Datta R, Chamusco K C, Chourey P S.2002. Starch biosynthesis during pollen maturation is associated with altered patterns of gene expression in maize. Plant Physiol,130:1645-1656
Deveshwar P, Bovill W D, Sharma R, Able J A, Kapoor S.2011. Analysis of anther transcriptomes to identify genes contributing to meiosis and male gametophyte development in rice. BMC Plant Biology, 11:78
Diaz I, Martinez M, Isabel-LaMoneda I, Rubio-Somoza I, Carbonero P.2005. The DOF protein, SAD, interacts with GAMYB in plant nuclei and activates transcription of endosperm-specific genes during barley seed development. Plant J,42:652-662
DosSantos P C, Smith A D, Frazzon J, Cash V L, Johnson M K, Dean D R.2004.Iron-sulfur cluster assembly:NifU-directed activation of the nitrogenase Fe protein. Journal of Biological Chemistry, 279(19):19705-19711
Dumas C, Berger F, Faure J-E, Matthys-Rochon E.1998. Gametes, fertilization and early embryogenesis in flowering plants. Advances in Botanical Research,28,231-261
Durbarry A, Vizir I, Twell D.2005. Male germ line development in Arabidopsis. duo pollen mutants reveal gametophytic regulators of generative cell cycle progression. Plant Physiol,137:297-307.
Durrieu C, Bernier-Valentin F, Rousset B.1987. Binding of glyceraldehyde 3-phosphate dehydrogenase to microtubules. Molecular Cell Biochemistry,74:55-65
Eady C, Lindsey K, Twel 1 D.1995. The significance of microspore division and division symmetry for vegetative cell-specific transcription and generative cell differentiation. Plant Cell,7:65-74
El-Ghazaly G, Jensen W A.1990. Development of wheat (Triticum aestivum) pollen wall before and after effect of a gametocide. Canadian Journal of Botany,68:2509-2516
Engel M L, Chaboud A, Dumas C, McCormick S.2003. Sperm cells of Zea mays have a complex complement of mRNAs. Plant J,34:697-707
Engel M, Seifert M, Theisinger B, Seyfert U, Welter C.1998. Glyceraldehyde-3-phosphate dehydrogenase and Nm23-H1/nucleoside diphosphate kinase A. two old enzymes combine for the novel Nm23 protein phosphotransferase function. Journal of Biological Chemistry,273:20058-20065
Fields S, Song O.1989. A novel genetic system to detect protein-protein interactions. Nature,340(6230): 245-246
Foussard M, Cabantous S, P6delacq J, Guillet V, Tranier S, Mourey L, Birck C, Samama J.2001. The molecular puzzle of two-component signaling cascades. Microbes Infect,3(5): 417-424
Goldberg R B, Beals T P, Sanders P M.1993. Anther development basic principles and practical applications. Plant Cell,5:1217-1229
Gonzalez-Melendi P, Testillano P S, Ahmadian P, Reyes J, Risueflo M C.2000. Immunoelectron microscopy of PCNA as an efficient marker for studying replication times and sites during pollen development. Chromosoma,109:397-409
Grof C P L, Winning B M, Scaysbrook T P, Hill S A, Leaver C.1995. Mitochondrial pyruvate dehydrogenase molecular cloning of the El a subunit and expression analysis. Plant Physiology,108: 1623-1629
Haglund K, Dikic I.2005. Ubiquitylation and cell signaling. EMBO Journal,24:3353-3359
Hernould M, Suharsono Zabaleta E, Carde J P, Litvak S, Araya A, Mouras A.1998. Impairment of tapetum and mitochondria in engineered male-sterile tobacco plants. Plant Mol Biol,36:499-508
Higaki T, Kojo K H, Hasezawa S.2010. Critical role of actin bundling in plant cell morphogenesis. Plant Signaling & Behavior,5:484-488
Hoffman N E, Ko K, Milkowski D, Pichersky E.1991. Isolation and characterization of tomato cDNA and genomic clones encoding the ubiquitin gene ubi3. Plant Molecular Biology,17:1189-1201
Honys D, Twell D.2003. Comparative analysis of the Arabidopsis pollen transcriptome. Plant Physiol,132: 640-652
Honys D, Twell D.2004. Transcriptome analysis of haploid male gametophyte development in Arabidopsis. Genome Biology,5(11):R85
Hu C D, Kerppola T K.2003. Simultaneous visualization of multiple protein inter actions in living cells using multicolor fluorescence complementation analysis. Nat Biotechnol,21:539-545
Huang B, Wu P M, Bowker-Kinley M M, Robert A, Harris R A.2002. Regulation of pyruvate dehydrogenase kinase expression by peroxisome proliferator-activated receptor-a ligands, glucocorticoids, and insulin. Diabetes,51:276-283
Igamberdiev A U, Kleczkowski L A.2001. Implications of adenylate kinase-governed equilibrium of adenylates on contents of free magnesium in plant cells and compartments. Biochem J,360:225-231
Ignoul S, Eggermont J.2005. CBS domains:structure, function, and pathology in human proteins. American Journal of Physiology-Cell Physiology,289:C1369-C1378
Ito T, Chiba T, Ozawa R, Yoshida M, Hattori M, Sakaki Y.2001. A comprehensive two-hybrid analysis to explore the yeast protein interactome. Proc Natl Acad Sci US A,98:4569-4574
Jach G, Pesch M, Richter K, Frings S, Uhrig J F.2006. An improved mRFP1 adds red to bimolecular fluorescence complementation. Nat Methods,3:597-600
Jan A, Nakamura H, Handa H, Ichikawa H, Matsumoto H, Komatsu S.2006. Gibberellin regulates mitochondrial pyruvate dehydrogenase activity in rice. Plant Cell Physiology,47(2):244-253
Johnson N, Varshavsky A.1994. Split ubiquitin as a sensor of protein interactions in vivo. Proc Nat Acad Sci U S A,91 (22):10340-10344
Kamalay J, Cgoldberg R B.1984. Organ-specific nuclear RNAs in tobacco. Proceedings of the National Academy Sciences USA,81:2801-2805
Kawalleck P, Somssich I E, Feldbrugge M, Hahlbrock K, Weisshaar B.1993. Polyubiquitin gene expression and structural properties of the ubi4-2 gene in Petroselium crispum. Plant Molecular Biology,21:673-684
Kerppola T K.2008. Bimolecular fluorescence complementation:visualization of molecular interactions in living cells. Methods Cell Biol,85:431-470
Kim H J, Oh S A, Brownfield L, Hong S H, Ryu H, Hwang I, Twell D, Nam H G.2008. Control of plant germline proliferation by SCFFBL17degradation of cell cycle inhibitors. Nature,455:1134-1137
Kim J W, Tchernyshyov I, Semenza G L, Dang C V.2006. HIF-1 mediated expression of pyruvate dehydrogenase kinase:a metabolic switch required for cellular adaptation to hypoxia. Cell Metabolism, 3:177-185
Kim T H, Park J H. Kim M C, Cho S H.2008. Cutin monomer induces expression of the rice OsLTP5 lipid transfer protein gene. Journal of Plant Physiology,165:345-349
Kimura S, Suzuki T, Yanagawa Y, Yamamoto T, Nakagawa H, Tanaka I, Hashimoto J, Sakaguchi K.2001. Characterization of plant proliferating cell nuclear antigen (PCNA) and flap endonuclease-1 (FEN-1), and their distribution in mitotic and meiotic cell cycles. Plant Journal,28:643-653
Kor6tchkina L G, Patel M S.2001. Probing the mechanism of inactivation of human pyruvate dehydrogenase by phosphorylation of three sites. J Biol Chem,276:5731-5738
Kumeta H, Ogura K, AsayamaM, Katoh S, Katoh E, Teshima K, Inagaki F.2007. The NMR structure of the domain Ⅱ of a chloroplastic NifU-like protein OsNifUlA. Journal of Biomolecular NMR,38(2): 161-164
Lalanne E, Twell D.2002. Genetic control of male germ unit organization in Arabidopsis. Plant Physiology,129:865-875
Laser K D, Lersten N R.1972. Anatomy and cytology of microsporogenesis in cytoplasmic male sterile angiosperms. Botanical Review,38(3):425-454
Lee J Y, Lee D H.2003. Use of serial analysis of gene expression technology to reveal changes in gene expression in Arabidopsis pollen undergoing cold stress. Plant Physiology,132:517-529
Lee S B, Go Y S, Bae H J, Park J H, Cho S H, Cho H J, Lee D S, Park O K, Hwang I, Suh M C.2009. Disruption of glycosylphosphatidylinositol-anchored lipid transfer protein gene altered cuticular lipid composition, increased plastoglobules, and enhanced susceptibility to infection by the fungal pathogen alternaria brassicicola. Plant Physiology,150:42-54
Lernmark U, Gardestrom P.1994. Distribution of pyruvate dehydrogenase complex activities between chloroplasts and mitochondria from leaves of different species. Plant Physiology,106:1633-1638
Licitra E, Liu J O.1996. A three-hybrid system for detecting small ligand-protein receptor interactions. Proc Natl Acad Sci USA,93:12817-12821
Liu Q, Kasuga M, Sakuma Y, Abe H, Miura S, Yamaguchi-Shinozaki K, Shinozaki K.1998. Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought and low-temperature-responsive gene expression, respectively, in Arabidopsis. Plant Cell,10:1391-1406
Liu Y Q Chen Y L.2007. High-efficiency thermal asymmetric interlaced PCR for amplification of unknown flanking sequences. Short Technical Reports,43 (5):649-656
Liu Y G, Whittier R F.1995. Thermal asymmetric interlaced PCR:automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking. Genomics,25: 674-681
Livak K J, Schmittgen T D.2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔct method. Methods,25:402-408
Lu S Y, Li Y F, Chen Z K, Lin J X.2003. Pollen development in Picea asperata Mast. Flora,198:112-117
Luethy M H, Miernyk J A, Randall D D.1995. The mitochondrial pyruvate dehydrogenase complex: nucleotide and deduced amino acid sequences of a cDNA encoding the Arabidopsis thaliana El a-subunit. Gene,164:251-254
Ma L G,Xu X D, Cui S J, Sun D Y.1999. The presence of a heterotrimeric G protein and its role in signal transduction of extracellular calmodulin in pollen development germination and tube growth. Plant Cell,11:1351-1363
Marillia E F, Micallef B J, Micallef M, Weninger A, Pedersen K K, Zou J T, Taylor D C.2003. Biochemical and physiological studies of Arabidopsis thaliana transgenic lines with repressed expression of the mitochondrial pyruvate dehydrogenase kinase. J Exp Bot,54:259-270
Marten K F, Wlkstr O M.1997. Proton pump coupled to cytochrome c oxidase in mitochondria. Nature, 266:271-273
Mazars G R, Moyret C, Jeanteur P, Theillet C G.1991. Direct sequencing by thermal asymmetric PCR. Nucleic Acids Res,19:4783
McCormick S.2004. Control of male gametophyte development. Plant Cell,16:S142-S153
McRae H H.1985. Advances in chemical hybridization. Plant breeding reviews,3:169-192
Meyer-Siegler K, Mauro D J, Seal G, Wurzer J, deRiel J K, Sirover M A.1991. A human nuclear uracil DNA glycosylase is the 37-kDa subunit of glyceraldehyde-3-phosphate dehydrogenase. Proceedings of the National Academy of Sciences,88:8460-8464
Millar A H, Leaver C J.2000. The cytotoxic lipid peroxidation product,4-hydroxy-2-nonenal, specifically inhibits decarboxylating dehydrogenases in the matrix of plant mitochondria. FEBS Lett,481: 117-121
Mizelle M B, Sethi R,Ashton M E, Jemen W A.1989. Development of the pollen grain and tapetum of wheat (Triticum aestirum) in untreated plants and plants treated with chemical hybridizing agent RH0007. Sex Plant Reprod,2:231-253
Munoz-Bertomeu J, Cascales-Minana B, Irles-Segura A, Mateu I, Nunes-Nesi A, Fernie A R, Segura J, Ros R.2010. The plastidial glyceraldehyde-3-phosphate dehydrogenase is critical for viable pollen development in Arabidopsis. Plant Physiology,152:1830-1841
Naryzhny S N.2008. Proliferating cell nuclear antigen:a proteomics view. Cellular and Molecular Life Sciences,65:3789-3808
Obrdlik P, El-Bakkoury M, Hamacher T, Cappellaro C, Vilarino C, Fleischer C, Ellerbrok H, Kamuzinzi R, Ledent V, Blaudez D, Sanders D, Revuelta J L, Boles E, Andr6 B, Frommer W B.2004. K+ channel interactions detected by a genetic system optimized for systematic studies of membrane protein interactions. Proc Natl Acad Sci U S A,101(33):12242-122427
Pacini E.1996. Types and meaning of pollen carbohydrate reserves. Sexual Plant Reproduction,9: 362-366
Papandreou I, Cairns R A, Fontana L, Lim A L, Denko N C.2006. HIF-1 mediates adaptation to hypoxia by actively down-regulating mitochondrial oxygen consumption. Cell Metabolism,3:187-197
Park S Y, Jauh G Y, Mollet J C, Eckard K J, Nothnagel E A, Walling L L, Lord E M.2000. A lipid transfer-like protein is necessary for lily pollen tube adhesion to an in vitro stylar matrix. Plant Cell Physiology,12:151-164
Peskan-Berghofer T, Neuwirth J, Kusnetsov V, Oelmuller R.2005. Suppression of heterotrimeric G-protein beta-subunit affects anther shape, pollen development and inflorescence architecture in tobacco. Planta, 220:737-746
Picard E, Hours C, Gregoire S, Phan T H, Meunier J P.1987. Significant improvement of androgenetic haploid and doubled haploid induction from wheat plants treated with a chemical hybridization agent. Ther Appl Genet,74:289-297
Randall D D, Miernyk J A, David N R, Gemel J, Luethy M H.1996. Regulation of leaf mitochondrial pyruvate dehydrogenase complex activity by reversible phosphorylation. Proc Phytochem Soc Eur,39: 87-103
Regan S M, Moffatt B A.1990. Cytochemical analysis of pollen development in wild-type Arabidopsis and a male-sterile mutant. Plant Cell,2:877-889
Reid E E,Thompson P, Lyttle C R, Dennis D T.1977. Pyruvate dehydrogenase complex from higher plants mitochondria and proplastids. Plant Physiology,59:842-848
Reinders A, Schulze W, Thaminy S, Stagljar I, Frommer W B, Ward J M.2002. Intra-and intermolecular interactions in sucrose transporters at the plasma membrane detected by the split-ubiquitin system and functional assays. Structure,10(3):763-772
Roche T E, Baker J C, Yan X, Hiromasa Y, Gong X, Peng T, Dong J, Turkan A, Kasten A.2001. Distinct regulatory properties of pyruvate dehydrogenase kinase and phosphatase isoforms. Prog Nucleic Acid Res Mol Biol,70:33-75
Rogers H J, Bate N, Combe J, Sullivan J, Sweetman J, Swan C, Lonsdale D M, Twell D.2001. Functional analysis of cis-regulatory elements within the promoter of the tobacco late pollen gene g10. Plant Molecular Biology,45:577-585
Rotman N, Durbarry A, Wardle A, Yang W C, Chaboud A,Faure J E, Berger F, Twell D.2005. A novel class of MYB factors controls sperm-cell formation in plants. Curr Biol,15:244-248
Rowell, P L, Miller D G 1971. Induction of male sterility in wheat with 2-chloroethyl-phosphonic acid (Ethrel). Crop Sci,11:629-631
Sanders P M, Bui A Q, Weterings K, McIntire K N, Hsu Y C, Lee P Y, Truong M T, Beals T P, Goldberg R B.1999. Anther developmental defects in Arabidopsis thaliana male-sterile mutants. Sex Plant Reprod, 11:297-322
Sarowar S, Kim Y J, Kim K D, Hwang B K, Ok S H, Shin J S.2009. Over-expression of lipid transfer protein (LTP) genes enhances resistance to plant pathogens and LTP functions in long-distance systemic signaling in tobacco. Plant Cell Report,28:419-427
Savchenko G, Wiese C, Neimanis S, Hedrich R, Heber U. pH regulation in apoplastic and cytoplasmic cell compartments of leaves. Planta,211:246-255
Schwacke R, Grallath S, Breitkreuz KE, Stransky E, Stransky H, Frommer WB, Rentsch D.1999. LeProT1, a transporter for proline, glycine betaine, and gamma-amino butyric acid in tomato pollen. Plant Cell,11:377-392
Scorah J, Dong M Q, Yates III J R, Scott M, Gillespie D, McGowan C H.2008. A conserved proliferating cell nuclear antigen-interacting protein sequence in Chkl is required for checkpoint function. Journal of Biological Chemistry,283:17250-17259
Scott R J, Spielman M, Dickinson H G. 2004. Stamen structure and function. Plant Cell,16:S46-S60
Senthil K, Devakumar C, Rajendra K, Tomar S M S.2009. Synthesis and evaluation of oxanilates and oxamates as chemical hybridizing agents for wheat(Triticum aestivum L.) Pesticide Research Journal, 21(2):133-139
Shi Y L, Zhao S, Yao J L.2009. Premature tapetum degeneration:a major cause of abortive pollen development in photoperiod sensitive genic male sterility in rice. Journal of Integrative Plant Biology, 51(8):774-781
Shuller, K A, Randall D D.1990. Mechanism of pyruvate inhibition of plant pyruvate dehydrogenase complex and synergism with ADP. Arch Biochem Biophys,278:211-216
Shyu Y J, Liu H, Deng X,Hu C D.2006. Identification of new fluorescent protein fragments for bimolecular fluorescence complementation analysis under physiological conditions. Biotechniques,40: 61-66
Simon M I, Strathmann M P, Gautam N.1991. Diversity of G proteins in signal transduction. Science,252: 802-808
Smalle J, Vierstra R D.2004. The ubiquitin 26S proteasome proteolytic pathway. Annual Review of Plant Biology,55:555-590
Smid O, Horakova E, Vilimova V, Hrdy I, Cammack R, Horvath A, Lukes J, Tachezy J.2006. Knock-downs of iron-sulfur cluster assembly proteins IscS and IscU down-regulate the active mitochondrion of procyclic Trypanosoma brucei. The Journal of Biological Chemistry,281: 28679-28686
Stagljar I, Korostensky C, Johnsson N, Heesen S T.1998. A genetic system based on split-ubiquitin for the analysis of interactions between membrane proteins in vivo. Proc Natl Acad Sci U S A,95 (9): 5187-5192
Stock A M, Robinson V L, Goudreau P N.2000. Two-component signal transduction. Annu Rev Biochem, 69:183-215
Strzalka W, Kaczmarek A, Naganowska B, Ziemienowicz A.2010. Identification and functional analysis of PCNA1 and PCNA-like1 genes of Phaseolus coccineus. Journal of Experimental Botany,61:873-888
Takasaki Y, Kaneda K, Matsushita M, Yamada H, Nawata M, Matsudaira R, Asano M, Mineki R, Shindo N, Hashimoto H.2004. Glyceraldehyde 3-phosphate dehydrogenase is a novel autoantigen leading autoimmune responses to proliferating cell nuclear antigen multiprotein complexes in lupus patients. International Immunology,16:1295-1304
Tanaka I.1997. Differentiation of generative and vegetative cells in angiosperm pollen. Sex Plant Reprod, 10:1-7
Tank J G, Thaker V S.2011. Cyclin dependent kinases and their role in regulation of plant cell cycle. Biologia Plantarum,55(2):201-212
Taylor P E, Glover J A, Lavithis M, Craig S, Singh M B, Knox R B, Dennis E S, Chaudhury A M.1998. Genetic control of male fertility in Arabidopsis thaliana:structural analyses of postmeiotic developmental mutants. Planta,205:492-505
Tedege M, Kuhlemeier, C.1997. Aerobic fermentation during tobacco pollen development. Plant Molecular Biology,35:3433-3454
Thelen J J, Miernyk J A, Randall D D.1999. Molecular cloning and expression analysis of the mitochondrial pyruvate dehydrogenase from maize. Plant Physiology,119:635-643
Thompson P, Reid E E, Lyttle C R, Dennis D T.1977. Pyruvate dehydrogenase complex from higher plant mitochondria and proplastids:regulation. Plant Physiology,59(5):854-858
Tong W H, Rouault T A.2006. Functions of mitochondrial ISCU and cytosolic ISCU in mammalian iron-sulfur cluster biogenesis and iron homeostasis. Cell Metabolism,3(3):199-210
Tover-Mendez A T, Miernyk J A, Randall D D.2003. Regulation of pyruvate dehydrogenase complex activity in plant cells. European Journal of Biochemistry,270:1043-1049
Twell D, Howden R.1998. Cell polarity, asymmetric division and cell fate determination in developing pollen. In Reproductive Biology. Edited by Owens, S.J. and Rudall, P.J. pp.197-218. Royal Botanic Gardens, Kew, UK.
Uchida K.2003.4-Hydroxy-2-nonenal:a product and mediator of oxidative stress. Prog Lipid Res,42: 318-343
Uetz, P, Giot L, Cagney G, Mansfield T A, Judson R S, Knight J R, Lockshon D, Narayan V, Srinivasan M, Pochart P, Qureshi-Emili A, Li Y, Godwin B, Conover D, Kalbfeisch T, Vijayadamodar G, Yang M J, Johnston M, Fields S, Rothberg J M.2000. A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae. Nature,403:623-627
Vanlerberghe G, Meintosh L.1996. Signals regulating the expression of the nuclear gene encoding altemative oxidase of plant mitoehondria. Plant physiology,111(2):589-595
Vidal M, Brachmann R K, Fattaey A, HarlowE, Boeke J D.I996. Reverse two-hybrid and one-hybrid systems to detect dissociation of protein-protein interactions. Proc Natl Acad Sci U S A, 93:10315-10320
Volker K W, Knull H.1997. A glycolytic enzyme binding domain on tubulin. Archives of Biochemistry Biophysics,338:237-243
Waadt R, Schmidt L K, Lohse M, Hashimoto K, Bock R,Kudla J.2008. Multicolor bimolecular fluorescence complementation reveals simultaneous formation of alternative CBL/CIPK complexes in planta. Plant J,56 (3):505-526
Walter M, Chaban C, Schutze K, Batistic O, Weckermann K, Nake C, Blazevic D, Grefen C, Schumacher K, Oecking C, Harter K, Kudla J.2004. Visualization of protein interactions in living plant cells using bimolecular fluorescence complementation. Plant J,40(3):428-4438
Wang M M, Reed R R.1993. Molecular cloning of the olfactory neuronal transcription factor Olf-1 by genetic selection in yeast. Nature,364(6433):121-126
Weinthal D, Tzfira T.2009. Imaging protein-protein interactions in plant cells by bimolecular fluorescence complementation assay. Trends in Plant Science,14(2):59-62
Wen L, Chase C D.1999. Mitochondrial gene expression in developing male gametophytes of male-fertile and S male-sterile maize. Sex Plant Reproduction,11:323-330
West A H, Stock A M.2001.Histidine kinases and response regulator proteins in two-component signaling systems. Trends Biochem Sci,26(6):369-376
Xie C T, Yang Y H, Qiu Y L, Zhu X Y, Tian H Q.2005. Cytochemical investigation of genetic male-sterility in chinese cabbage. Sex Plant Reproduction,18:75-80
Xu C H, Liu Z T, Zhang L P, Zhao C P, Yuan S H, Zhang F T.2012. Organization of actin cytoskeleton during meiosis I in a wheat thermo-sensitive genic male sterile line. Protoplasma (Published online)
Yamaguchi-Shinozaki K, Shinozaki K.1994. A novelcis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress. Plant Cell,6:251-264
Yan L F, Liu G Q, Xiao X G. 2000. From pollen actin to crop male sterility. Chinese Science Bulletin,45: 784-788
Yui R, Iketani S, Mikami T, Kubo T.2003. Atisense inhibition of mitochondrial pyruvate dehydrogenase El [alpha] subunit in anther tapetum causes male sterility. Plant J,34:57-66
Zhang D S, Liang W Q, Yin C, Zong J, Gu F W, Zhang D B.2010a. OsC6, encoding a lipid transfer protein, is required for postmeiotic anther development in rice. Plant Physiology,154:149-162
Zhang D S, Liang W Q, Yuan Z, Li N, Shi J, Wang J, Liu Y M, Yu W J, Zhang D B.2008. Tapetum degeneration retardation is critical for aliphatic metabolism and gene regulation during rice pollen development. Mol Plant,1(4):599-610
Zhang Q R, Teng H, Sun Y Q, Xiu Z L, Zeng A P.2008. Metabolic flux and robustness analysis of glycerol metabolism in Klebsiella pneumoniae. Bioprocess and Biosystems Engineering,31(2):127-135
Zheng L, Roeder R G, Luo Y.2003. S-phase activation of the histone H2B promoter by OCA-S, a coactivator complex that contains GAPDH as a key component. Cell,114:255-266
Zheng Z, Xia Q, Dauk M, Shen W, Selvaraj G, Zou J.2003. Arabidopsis AtGPAT1, a member of the membrane-bound glycerol-3-phosphate acyltransferase gene family, is essential for tapetum differentiation and male fertility. Plant Cell,15:1872-1887
Zou J T, Qi Q G, Katavic V, Marillia E F, Taylor D C.1999. Effects of antisense repression of an Arabidopsis thaliana pyruvate dehydrogenase kinase cDNA on plant development. Plant Mol Biol,41: 837-849
陈蕊红,叶景秀,张改生,王书平,李莉,牛娜,朱建楚.2009.小麦质核互作型雄性不育系及其保持系花药蛋白质组分研究与分析.生物化学与生物物理进展,36(4)：431-440
程春生,陆英飞,张改生,张宗俭,李鹏,张宝砚.2004.杀雄嗪酸的合成及其生物活性的
研究.农药,43(7)：315-317
崔秀辉.2008.化学杂交剂SQ-1诱导糜子雄性不育效果研究.作物学报,34(1)：106-110
韩艳芬,张龙雨,胡俊敏,张改生,李亚鑫,盛英,位芳,牛娜,马守才.2010.黏类小麦细胞质雄性不育线粒体atp6基因转录本编辑位点.作物学报,36(12)：2179-2184
侯国裕,黄荣初,王兴凤,杨师柏.1981.新化学杀雄剂KRIS-1的合成.农药,5：32-33
黄纯农,徐敏源,庄元忠.1981.乙烯利诱导的雄性不育小麦花粉发育的电子显微镜研究.植物学报(英文版),1：1-19
菅记涌,张勇,朱大海.2008.丙酮酸脱氢酶激酶的研究进展.基础医学与临床,28(11)：1212-1215
蒋明亮,陈万义.1995.哒嗪类植物化学杂交剂的研究概况.农药译丛,17(3)：42-44
类延宝,宋松泉,傅家瑞,程红焱.2004.植物的双组分信号系统.植物学通报,21(2)：216-227
李莉,王书平,张改生,王亮明,宋瑜龙,张龙雨,牛娜,马守才.2011.小麦生理型和遗传型雄性不育系及其保持系小花完整叶绿体蛋白质组分比较研究.作物学报,37(7)：1134-1143
李小琴,万邦惠,刘纪麟,郑用琏,李建生,徐尚忠.2004.玉米新不育胞质WBMs小孢子败育的细胞学观察.中国农业科学,37(9)：1261-1264
李亚鑫,盛英,张改生,宋瑜龙,王亮明,张龙雨,王书平,李莉.2011.杀雄剂SQ-1诱导的小麦生理型雄性不育系交替氧化酶基因(AOX1)的表达分析.麦类作物学报,31(5)：805-810
刘宏伟,张改生,刘秉华.2004.化学杂交剂GENESIS诱导小麦雄性不育的细胞形态学观察.西北植物学报,24(12)：2282-2285
刘宏伟,张改生,王军卫,王小利,方正武.2003.化学杂交剂SQ-1诱导小麦雄性不育及与不同小麦品种互作效应的研究.西北农林科技大学学报(自然科学版),31(4)：15-18
刘永生,张改生,牛娜.2007.化学杂交剂SQ-1诱导雄性不育小麦开颖特性的研究.麦类作物学报,27(3)：442-444
裴阿卫.1996.化学杂交剂WL84811诱导普通小麦雄性不育时花粉的细胞形态学观察.西北农业学报,5(3)：93-94
彭丹妮,黄静,吴自荣.2007.酵母三杂交系统的原理和应用.生命科学,19(4)：461-464
漆正堂,丁树哲,贺杰.2008.丙酮酸脱氢酶活性调控在线粒体代谢与生长平衡中的作用.生命的化学,28(3)：307-310
沈银柱,刘植义,黄占景,何聪芬,柏峰,马闻师,朱正歌,葛荣朝,许顺芝,岳聚兴,张中良.1999.不同化学杂交剂(CHA)对小麦花药同工酶影响的研究.遗传,21(5)：41-46
苏俊英,吕德彬,程西永,许海霞.2000.化学杂交剂Genesis及Sc2053对小麦穗部过氧化物酶活性的影响.河南农业大学学报,34(4)：309-311
谭才邓.2007.植物丙酮酸脱氢酶系及其调节酶的研究进展.广西轻工业,6：7-9
田爱梅,曹家树.2008.植物脂质转移蛋白.细胞生物学杂志,30：483-488
王涛,贺红武,袁均林.2003.丙酮酸脱氢酶系：除草剂品种的新靶标.应用化学,20(7)：613-617
王俊生,袁蕾,张明珠,张改生,牛娜,马守才,李红霞.2010.化学杂交剂SQ-1诱导小麦泛素/26S蛋白酶体途径的表达.农业生物技术学报,18(4)：695-701
王俊生,张改生,原蕾,张明珠,牛娜,马守才,叶景秀.2009a.化学杂交剂诱导的小麦生理型雄性不育花药的活性氧代谢.西北植物学报,29(7)：1351-1357
王俊生.2009b.小麦生理型雄性不育花药活性氧代谢和基因表达分析.[博士学位论文].杨凌：西北农林科技大学
位明明,王俊生,张改生,张龙雨,袁正杰,孙瑞,叶景秀,牛娜,马守才,李红霞.2009.GAPDH基因表达与小麦生理型雄性不育花药败育的关系。分子植物育种,7(4)：679-684
王书平,张改生,叶景秀,李莉,宋瑜龙,王亮明,代军军,张龙雨,牛娜,马守才,朱建楚.2010.杀雄剂SQ-1诱导小麦生理型雄性不育小花完整叶绿体差异蛋白质的鉴定.中国生物化学与分子生物学报,26(9)：854-861
王书文,赵寅槐,王艳平,张爱民,王道全,陈万义,黄铁城,王茂江.2000.化学杂交剂BAU9403诱导小麦雄性不育研究初报.江苏农业科学,6：18-20
王永军,张改生,王军卫,牛娜.2005.小麦遗传型与生理型雄性不育花药同工酶的比较研究.麦类作物学报,25(4)：44-49
王振华,刘宏伟,张改生,王军卫,马守才.2003.3种新型化学杂交剂诱导小麦雄性不育效果比较.西北农林科技大学学报(自然科学版),31(3)：43-46
肖建国,蒋爱湘,冯贵苓,石斯发,徐昕,冯刚,周之杭,梁咏东,尤文玲.1996.化学杂交剂“津奥啉”诱导小麦雄性不育机理研究.华北农学报,11(4)：7-11
闫先喜,梁作勤,田纪春.1996.Sc2053诱导小麦雄性不育形态学和细胞学观察.华北农学报,1(1)：19-24
杨涛.2004.化学杂交剂诱导的小麦雄性不育育性相关基因片段的分离与克隆.[中国农业大学].北京：硕士学位论文
姚雅琴,杨天章.1996.K型小麦雄性不育系花药壁及小孢子发育特征.电子显微学报,15(5)：402
叶景秀,陈蕊红,张改生,王俊生,王书平,李莉,牛娜,马守才,李红霞,朱建楚.2009a.杀雄剂SQ-1诱导小麦雄性不育花药蛋白质组分分析.农业生物技术学报,17(5)：858-864
叶景秀,张改生,王书平,陈蕊红,王俊生,牛娜,马守才,李红霞,朱建楚.2009b.杀雄剂SQ-1诱导小麦雄性不育花粉粒差异蛋白质组学研究.中国生物化学与分子生物学报,25(10)：949-957
张龙雨,袁蕾,杨书玲,张改生,王俊生,宋瑜龙,赵卓军,牛娜,马守才.2011.小麦雄性不育系中TaPDC-E1α及其调节酶基因的表达特征.作物学报,37(4)：620-628
张明珠,袁蕾,王俊生,张改生,郭文江,张龙雨.2010.顺乌头酸酶基因在杀雄剂SQ-1诱导小麦雄性不育花药中的表达分析.中国生物化学与分子生物学报,26(8)：740-748
周江菊,陈良碧,龙跃生.2001.双低两用温敏核不育水稻低温条件下花粉发育的细胞学观察.生命科学研究,5(2)：160-163
朱树勇,迟伟,张立新.2006.分裂泛素化酵母双杂交系统研究光合膜蛋白相互作用.生物化学与生物物理进展,33(4)：350-356
Araki M, Nozaki Y, Motojima K.2007. Transcriptional regulation of metabolic switching PDK4 gene under various physiological conditions. Yakugaku Zasshi,127:153-162
Ariizumi T, M Amagai, D Shibata, K Hatakeyama, M Watanabe, K Toriyama.2002. Comparative study of promoter activity of three anther-specific genes encoding lipid transfer protein, xyloglucan endotransglucosylase/hydrolase and polygalacturonase in transgenic Arabidopsis thaliana. Plant Cell Report,21:90-96.
Bateman A.1997. The structure of a domain common to archaebacteria and the homocystinuria disease protein. Trends in Biochemical Sciences,22:12-13
Baxi M D, Vishwanatha J K.1995. Uracil DNA-glycosylase/glyceraldehyde-3-phosphate dehydrogenase is an Ap4A binding protein. Biochemistry,34:9700-9707
Binet N N, Wei J H, Tessier L H.1991. Structure and expression of sunflower ubiquitin genes. Plant Molecular Biology,17:395-470
Boavida L C, Becker J D, Feijo J A.2005. The making of gametes in higher plants. Int J Dev Biol.49: 595-614
Borg M, Brownfield L, Twell D.2009. Male gametophyte development:a molecular perspective. Journal of Experimental Botany,60(5):1465-1478
Bourret R B, Borkovich K A, Simon M I.1991. Signal transduction pathways involving protein phosphorylation in prokaryotes. Annu Rev Biochem,60:401-441
Boutrot F, Meynard D, Guiderdoni E, Joudrier P, Gautier M F.2007. The Triticum aestivum non-specific lipid transfer protein (TaLtp) gene family:comparative promoter activity of six TaLtp genes in transgenic rice. Planta,225:843-862
Bravo R, Fey S J, Bellatin J, Larsen P M, Celis J E.1982. Identification of nuclear polypeptide ('cyclin') whose relative proportion is sensitive to changes in the rate of cell proliferation and to transformation. Progress in Clinical and Biological Research,85:235-248
Bryksin A V, Laktionov P P.2008. Role of glyceraldehyde-3-phosphate dehydrogenase in vesicular transport from golgi apparatus to endoplasmic reticulum. Biochemistry,73:619-625
Budde R J A, Fang T K, Randall D D.1988. Regulation of the phosphorylation of mitochondrial pyruvate dehydrogenase complex in situ. Plant Physiology,88:1031-1036
Budde R J A, Randall D D.1990. Pea leaf mitochondrial pyruvate dehydrogenase complex is inactivated in vivo in a light-dependent manner. Proceedings of the National Academy of Sciences,8:673-676
Budde R J, Fang T K, Randall D D, Miernyk J A.1991. Acetyl-Coenzyme A can regulate activity of the mitochondrial pyruvate dehydrogenase complex in situ. Plant Physiol,95:131-136
Camp P J, Miernyk J A, Randall D D.1988. Some kinetic and regulatory properties of the pea chloroplast pyruvate dehydrogenase complex. Biochim Biophys Acta,933:269-275
Camp P J, Randall D D.1985. Purification and characterization of the pea chloroplast pyruvate dehydrogenase complex:a source of acetyl-CoA and NADH for fatty acid biosynthesis. Plant Physiol, 77:571-577
Caplan J L, Mamillapalli P, Burch-Smith T M, Czymmek K, Dinesh-Kumar S P.2008. Chloroplastic protein NRIP1 mediates innate immune receptor recognition of a viral effector. Cell,132:449-462
Chakraborty K, Devakumar C, Tomar S M S, Kumar R.2003. Synthesis and quantitative structure-activity relationships of oxanilates as chemical hybridizing agents for wheat(Triticum aestivum L). Journal of agriculture and food chemistry,51(4):992-998
Chakraborty K, Devakumar C.2006a. Ethyloxanilates as specific male gametocides for wheat (Triticum aestivum L.). Plant Breeding,125:441-447
Chakraborty K, Devakumar C.2006b. Synthesis and screening of 5-carbethoxy-N-aryl-4,6-dimethyl-1,2-dihydrophrid-2-ones as chemical hybridizing agents for wheat (Triticum aestivum L.). Indial Journal of Chemistry,45B:703-714
Chakraborty K, Devakumar C.2005. N-acylanilines, herbicide-CHA chimera, and amino acid analogues as novel chemical hybridizing agents for wheat (Triticum aestivum L.). Journal of agriculture and food chemistry,53(20):7899-7907
Chakraborty K, Devakumar C.2006c. Evaluation of chemical compounds for induction of male sterility in wheat (Triticum aestivum L.). Euphytica,147:329-335
Chang L, Ma H, Xue H W.2009. Functional conservation of the meiotic genes SDS and RCK in male meiosis in the momocot rice. Cell Res,19(6):768-782
Christensen A H, Sharrok R A, Quail P H.1992. Maize polyubiquitin genes:structure, thermal perturbation of expression and transcript splicing, and promoter activity following transfer to protoplasts by electroporation. Plant Molecular Biology,18:675-689
Citovsky V, Gafni Y, Tzfira T.2008. Localizing protein-protein interactions by bimolecular fluorescence complementation in planta. Methods,45(3):196-206
Collantes E R, Xing L, Miller P C, Welsh W J, Profeta S J.1999. Comparative molecular field analysis as a tool to evaluate mode of action of chemical hybridization agents. Journal of agriculture and food chemistry,47(12):5245-5251
Currier H B, Day B E, Crafts A S.1951. Some effects of maleic hydrazide on plants. Botanical Gazette, 112(3):271-280
Datta R, Chamusco K C, Chourey P S.2002. Starch biosynthesis during pollen maturation is associated with altered patterns of gene expression in maize. Plant Physiol,130:1645-1656
Deveshwar P, Bovill W D, Sharma R, Able J A, Kapoor S.2011. Analysis of anther transcriptomes to identify genes contributing to meiosis and male gametophyte development in rice. BMC Plant Biology, 11:78
Diaz I, Martinez M, Isabel-LaMoneda I, Rubio-Somoza I, Carbonero P.2005. The DOF protein, SAD, interacts with GAMYB in plant nuclei and activates transcription of endosperm-specific genes during barley seed development. Plant J,42:652-662
DosSantos P C, Smith A D, Frazzon J, Cash V L, Johnson M K, Dean D R.2004.Iron-sulfur cluster assembly:NifU-directed activation of the nitrogenase Fe protein. Journal of Biological Chemistry, 279(19):19705-19711
Dumas C, Berger F, Faure J-E, Matthys-Rochon E.1998. Gametes, fertilization and early embryogenesis in flowering plants. Advances in Botanical Research,28,231-261
Durbarry A, Vizir I, Twell D.2005. Male germ line development in Arabidopsis. duo pollen mutants reveal gametophytic regulators of generative cell cycle progression. Plant Physiol,137:297-307.
Durrieu C, Bernier-Valentin F, Rousset B.1987. Binding of glyceraldehyde 3-phosphate dehydrogenase to microtubules. Molecular Cell Biochemistry,74:55-65
Eady C, Lindsey K, Twel 1 D.1995. The significance of microspore division and division symmetry for vegetative cell-specific transcription and generative cell differentiation. Plant Cell,7:65-74
El-Ghazaly G, Jensen W A.1990. Development of wheat (Triticum aestivum) pollen wall before and after effect of a gametocide. Canadian Journal of Botany,68:2509-2516
Engel M L, Chaboud A, Dumas C, McCormick S.2003. Sperm cells of Zea mays have a complex complement of mRNAs. Plant J,34:697-707
Engel M, Seifert M, Theisinger B, Seyfert U, Welter C.1998. Glyceraldehyde-3-phosphate dehydrogenase and Nm23-H1/nucleoside diphosphate kinase A. two old enzymes combine for the novel Nm23 protein phosphotransferase function. Journal of Biological Chemistry,273:20058-20065
Fields S, Song O.1989. A novel genetic system to detect protein-protein interactions. Nature,340(6230): 245-246
Foussard M, Cabantous S, P6delacq J, Guillet V, Tranier S, Mourey L, Birck C, Samama J.2001. The molecular puzzle of two-component signaling cascades. Microbes Infect,3(5): 417-424
Goldberg R B, Beals T P, Sanders P M.1993. Anther development basic principles and practical applications. Plant Cell,5:1217-1229
Gonzalez-Melendi P, Testillano P S, Ahmadian P, Reyes J, Risueflo M C.2000. Immunoelectron microscopy of PCNA as an efficient marker for studying replication times and sites during pollen development. Chromosoma,109:397-409
Grof C P L, Winning B M, Scaysbrook T P, Hill S A, Leaver C.1995. Mitochondrial pyruvate dehydrogenase molecular cloning of the El a subunit and expression analysis. Plant Physiology,108: 1623-1629
Haglund K, Dikic I.2005. Ubiquitylation and cell signaling. EMBO Journal,24:3353-3359
Hernould M, Suharsono Zabaleta E, Carde J P, Litvak S, Araya A, Mouras A.1998. Impairment of tapetum and mitochondria in engineered male-sterile tobacco plants. Plant Mol Biol,36:499-508
Higaki T, Kojo K H, Hasezawa S.2010. Critical role of actin bundling in plant cell morphogenesis. Plant Signaling & Behavior,5:484-488
Hoffman N E, Ko K, Milkowski D, Pichersky E.1991. Isolation and characterization of tomato cDNA and genomic clones encoding the ubiquitin gene ubi3. Plant Molecular Biology,17:1189-1201
Honys D, Twell D.2003. Comparative analysis of the Arabidopsis pollen transcriptome. Plant Physiol,132: 640-652
Honys D, Twell D.2004. Transcriptome analysis of haploid male gametophyte development in Arabidopsis. Genome Biology,5(11):R85
Hu C D, Kerppola T K.2003. Simultaneous visualization of multiple protein inter actions in living cells using multicolor fluorescence complementation analysis. Nat Biotechnol,21:539-545
Huang B, Wu P M, Bowker-Kinley M M, Robert A, Harris R A.2002. Regulation of pyruvate dehydrogenase kinase expression by peroxisome proliferator-activated receptor-a ligands, glucocorticoids, and insulin. Diabetes,51:276-283
Igamberdiev A U, Kleczkowski L A.2001. Implications of adenylate kinase-governed equilibrium of adenylates on contents of free magnesium in plant cells and compartments. Biochem J,360:225-231
Ignoul S, Eggermont J.2005. CBS domains:structure, function, and pathology in human proteins. American Journal of Physiology-Cell Physiology,289:C1369-C1378
Ito T, Chiba T, Ozawa R, Yoshida M, Hattori M, Sakaki Y.2001. A comprehensive two-hybrid analysis to explore the yeast protein interactome. Proc Natl Acad Sci US A,98:4569-4574
Jach G, Pesch M, Richter K, Frings S, Uhrig J F.2006. An improved mRFP1 adds red to bimolecular fluorescence complementation. Nat Methods,3:597-600
Jan A, Nakamura H, Handa H, Ichikawa H, Matsumoto H, Komatsu S.2006. Gibberellin regulates mitochondrial pyruvate dehydrogenase activity in rice. Plant Cell Physiology,47(2):244-253
Johnson N, Varshavsky A.1994. Split ubiquitin as a sensor of protein interactions in vivo. Proc Nat Acad Sci U S A,91 (22):10340-10344
Kamalay J, Cgoldberg R B.1984. Organ-specific nuclear RNAs in tobacco. Proceedings of the National Academy Sciences USA,81:2801-2805
Kawalleck P, Somssich I E, Feldbrugge M, Hahlbrock K, Weisshaar B.1993. Polyubiquitin gene expression and structural properties of the ubi4-2 gene in Petroselium crispum. Plant Molecular Biology,21:673-684
Kerppola T K.2008. Bimolecular fluorescence complementation:visualization of molecular interactions in living cells. Methods Cell Biol,85:431-470
Kim H J, Oh S A, Brownfield L, Hong S H, Ryu H, Hwang I, Twell D, Nam H G.2008. Control of plant germline proliferation by SCFFBL17degradation of cell cycle inhibitors. Nature,455:1134-1137
Kim J W, Tchernyshyov I, Semenza G L, Dang C V.2006. HIF-1 mediated expression of pyruvate dehydrogenase kinase:a metabolic switch required for cellular adaptation to hypoxia. Cell Metabolism, 3:177-185
Kim T H, Park J H. Kim M C, Cho S H.2008. Cutin monomer induces expression of the rice OsLTP5 lipid transfer protein gene. Journal of Plant Physiology,165:345-349
Kimura S, Suzuki T, Yanagawa Y, Yamamoto T, Nakagawa H, Tanaka I, Hashimoto J, Sakaguchi K.2001. Characterization of plant proliferating cell nuclear antigen (PCNA) and flap endonuclease-1 (FEN-1), and their distribution in mitotic and meiotic cell cycles. Plant Journal,28:643-653
Kor6tchkina L G, Patel M S.2001. Probing the mechanism of inactivation of human pyruvate dehydrogenase by phosphorylation of three sites. J Biol Chem,276:5731-5738
Kumeta H, Ogura K, AsayamaM, Katoh S, Katoh E, Teshima K, Inagaki F.2007. The NMR structure of the domain Ⅱ of a chloroplastic NifU-like protein OsNifUlA. Journal of Biomolecular NMR,38(2): 161-164
Lalanne E, Twell D.2002. Genetic control of male germ unit organization in Arabidopsis. Plant Physiology,129:865-875
Laser K D, Lersten N R.1972. Anatomy and cytology of microsporogenesis in cytoplasmic male sterile angiosperms. Botanical Review,38(3):425-454
Lee J Y, Lee D H.2003. Use of serial analysis of gene expression technology to reveal changes in gene expression in Arabidopsis pollen undergoing cold stress. Plant Physiology,132:517-529
Lee S B, Go Y S, Bae H J, Park J H, Cho S H, Cho H J, Lee D S, Park O K, Hwang I, Suh M C.2009. Disruption of glycosylphosphatidylinositol-anchored lipid transfer protein gene altered cuticular lipid composition, increased plastoglobules, and enhanced susceptibility to infection by the fungal pathogen alternaria brassicicola. Plant Physiology,150:42-54
Lernmark U, Gardestrom P.1994. Distribution of pyruvate dehydrogenase complex activities between chloroplasts and mitochondria from leaves of different species. Plant Physiology,106:1633-1638
Licitra E, Liu J O.1996. A three-hybrid system for detecting small ligand-protein receptor interactions. Proc Natl Acad Sci USA,93:12817-12821
Liu Q, Kasuga M, Sakuma Y, Abe H, Miura S, Yamaguchi-Shinozaki K, Shinozaki K.1998. Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought and low-temperature-responsive gene expression, respectively, in Arabidopsis. Plant Cell,10:1391-1406
Liu Y Q Chen Y L.2007. High-efficiency thermal asymmetric interlaced PCR for amplification of unknown flanking sequences. Short Technical Reports,43 (5):649-656
Liu Y G, Whittier R F.1995. Thermal asymmetric interlaced PCR:automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking. Genomics,25: 674-681
Livak K J, Schmittgen T D.2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔct method. Methods,25:402-408
Lu S Y, Li Y F, Chen Z K, Lin J X.2003. Pollen development in Picea asperata Mast. Flora,198:112-117
Luethy M H, Miernyk J A, Randall D D.1995. The mitochondrial pyruvate dehydrogenase complex: nucleotide and deduced amino acid sequences of a cDNA encoding the Arabidopsis thaliana El a-subunit. Gene,164:251-254
Ma L G,Xu X D, Cui S J, Sun D Y.1999. The presence of a heterotrimeric G protein and its role in signal transduction of extracellular calmodulin in pollen development germination and tube growth. Plant Cell,11:1351-1363
Marillia E F, Micallef B J, Micallef M, Weninger A, Pedersen K K, Zou J T, Taylor D C.2003. Biochemical and physiological studies of Arabidopsis thaliana transgenic lines with repressed expression of the mitochondrial pyruvate dehydrogenase kinase. J Exp Bot,54:259-270
Marten K F, Wlkstr O M.1997. Proton pump coupled to cytochrome c oxidase in mitochondria. Nature, 266:271-273
Mazars G R, Moyret C, Jeanteur P, Theillet C G.1991. Direct sequencing by thermal asymmetric PCR. Nucleic Acids Res,19:4783
McCormick S.2004. Control of male gametophyte development. Plant Cell,16:S142-S153
McRae H H.1985. Advances in chemical hybridization. Plant breeding reviews,3:169-192
Meyer-Siegler K, Mauro D J, Seal G, Wurzer J, deRiel J K, Sirover M A.1991. A human nuclear uracil DNA glycosylase is the 37-kDa subunit of glyceraldehyde-3-phosphate dehydrogenase. Proceedings of the National Academy of Sciences,88:8460-8464
Millar A H, Leaver C J.2000. The cytotoxic lipid peroxidation product,4-hydroxy-2-nonenal, specifically inhibits decarboxylating dehydrogenases in the matrix of plant mitochondria. FEBS Lett,481: 117-121
Mizelle M B, Sethi R,Ashton M E, Jemen W A.1989. Development of the pollen grain and tapetum of wheat (Triticum aestirum) in untreated plants and plants treated with chemical hybridizing agent RH0007. Sex Plant Reprod,2:231-253
Munoz-Bertomeu J, Cascales-Minana B, Irles-Segura A, Mateu I, Nunes-Nesi A, Fernie A R, Segura J, Ros R.2010. The plastidial glyceraldehyde-3-phosphate dehydrogenase is critical for viable pollen development in Arabidopsis. Plant Physiology,152:1830-1841
Naryzhny S N.2008. Proliferating cell nuclear antigen:a proteomics view. Cellular and Molecular Life Sciences,65:3789-3808
Obrdlik P, El-Bakkoury M, Hamacher T, Cappellaro C, Vilarino C, Fleischer C, Ellerbrok H, Kamuzinzi R, Ledent V, Blaudez D, Sanders D, Revuelta J L, Boles E, Andr6 B, Frommer W B.2004. K+ channel interactions detected by a genetic system optimized for systematic studies of membrane protein interactions. Proc Natl Acad Sci U S A,101(33):12242-122427
Pacini E.1996. Types and meaning of pollen carbohydrate reserves. Sexual Plant Reproduction,9: 362-366
Papandreou I, Cairns R A, Fontana L, Lim A L, Denko N C.2006. HIF-1 mediates adaptation to hypoxia by actively down-regulating mitochondrial oxygen consumption. Cell Metabolism,3:187-197
Park S Y, Jauh G Y, Mollet J C, Eckard K J, Nothnagel E A, Walling L L, Lord E M.2000. A lipid transfer-like protein is necessary for lily pollen tube adhesion to an in vitro stylar matrix. Plant Cell Physiology,12:151-164
Peskan-Berghofer T, Neuwirth J, Kusnetsov V, Oelmuller R.2005. Suppression of heterotrimeric G-protein beta-subunit affects anther shape, pollen development and inflorescence architecture in tobacco. Planta, 220:737-746
Picard E, Hours C, Gregoire S, Phan T H, Meunier J P.1987. Significant improvement of androgenetic haploid and doubled haploid induction from wheat plants treated with a chemical hybridization agent. Ther Appl Genet,74:289-297
Randall D D, Miernyk J A, David N R, Gemel J, Luethy M H.1996. Regulation of leaf mitochondrial pyruvate dehydrogenase complex activity by reversible phosphorylation. Proc Phytochem Soc Eur,39: 87-103
Regan S M, Moffatt B A.1990. Cytochemical analysis of pollen development in wild-type Arabidopsis and a male-sterile mutant. Plant Cell,2:877-889
Reid E E,Thompson P, Lyttle C R, Dennis D T.1977. Pyruvate dehydrogenase complex from higher plants mitochondria and proplastids. Plant Physiology,59:842-848
Reinders A, Schulze W, Thaminy S, Stagljar I, Frommer W B, Ward J M.2002. Intra-and intermolecular interactions in sucrose transporters at the plasma membrane detected by the split-ubiquitin system and functional assays. Structure,10(3):763-772
Roche T E, Baker J C, Yan X, Hiromasa Y, Gong X, Peng T, Dong J, Turkan A, Kasten A.2001. Distinct regulatory properties of pyruvate dehydrogenase kinase and phosphatase isoforms. Prog Nucleic Acid Res Mol Biol,70:33-75
Rogers H J, Bate N, Combe J, Sullivan J, Sweetman J, Swan C, Lonsdale D M, Twell D.2001. Functional analysis of cis-regulatory elements within the promoter of the tobacco late pollen gene g10. Plant Molecular Biology,45:577-585
Rotman N, Durbarry A, Wardle A, Yang W C, Chaboud A,Faure J E, Berger F, Twell D.2005. A novel class of MYB factors controls sperm-cell formation in plants. Curr Biol,15:244-248
Rowell, P L, Miller D G 1971. Induction of male sterility in wheat with 2-chloroethyl-phosphonic acid (Ethrel). Crop Sci,11:629-631
Sanders P M, Bui A Q, Weterings K, McIntire K N, Hsu Y C, Lee P Y, Truong M T, Beals T P, Goldberg R B.1999. Anther developmental defects in Arabidopsis thaliana male-sterile mutants. Sex Plant Reprod, 11:297-322
Sarowar S, Kim Y J, Kim K D, Hwang B K, Ok S H, Shin J S.2009. Over-expression of lipid transfer protein (LTP) genes enhances resistance to plant pathogens and LTP functions in long-distance systemic signaling in tobacco. Plant Cell Report,28:419-427
Savchenko G, Wiese C, Neimanis S, Hedrich R, Heber U. pH regulation in apoplastic and cytoplasmic cell compartments of leaves. Planta,211:246-255
Schwacke R, Grallath S, Breitkreuz KE, Stransky E, Stransky H, Frommer WB, Rentsch D.1999. LeProT1, a transporter for proline, glycine betaine, and gamma-amino butyric acid in tomato pollen. Plant Cell,11:377-392
Scorah J, Dong M Q, Yates III J R, Scott M, Gillespie D, McGowan C H.2008. A conserved proliferating cell nuclear antigen-interacting protein sequence in Chkl is required for checkpoint function. Journal of Biological Chemistry,283:17250-17259
Scott R J, Spielman M, Dickinson H G. 2004. Stamen structure and function. Plant Cell,16:S46-S60
Senthil K, Devakumar C, Rajendra K, Tomar S M S.2009. Synthesis and evaluation of oxanilates and oxamates as chemical hybridizing agents for wheat(Triticum aestivum L.) Pesticide Research Journal, 21(2):133-139
Shi Y L, Zhao S, Yao J L.2009. Premature tapetum degeneration:a major cause of abortive pollen development in photoperiod sensitive genic male sterility in rice. Journal of Integrative Plant Biology, 51(8):774-781
Shuller, K A, Randall D D.1990. Mechanism of pyruvate inhibition of plant pyruvate dehydrogenase complex and synergism with ADP. Arch Biochem Biophys,278:211-216
Shyu Y J, Liu H, Deng X,Hu C D.2006. Identification of new fluorescent protein fragments for bimolecular fluorescence complementation analysis under physiological conditions. Biotechniques,40: 61-66
Simon M I, Strathmann M P, Gautam N.1991. Diversity of G proteins in signal transduction. Science,252: 802-808
Smalle J, Vierstra R D.2004. The ubiquitin 26S proteasome proteolytic pathway. Annual Review of Plant Biology,55:555-590
Smid O, Horakova E, Vilimova V, Hrdy I, Cammack R, Horvath A, Lukes J, Tachezy J.2006. Knock-downs of iron-sulfur cluster assembly proteins IscS and IscU down-regulate the active mitochondrion of procyclic Trypanosoma brucei. The Journal of Biological Chemistry,281: 28679-28686
Stagljar I, Korostensky C, Johnsson N, Heesen S T.1998. A genetic system based on split-ubiquitin for the analysis of interactions between membrane proteins in vivo. Proc Natl Acad Sci U S A,95 (9): 5187-5192
Stock A M, Robinson V L, Goudreau P N.2000. Two-component signal transduction. Annu Rev Biochem, 69:183-215
Strzalka W, Kaczmarek A, Naganowska B, Ziemienowicz A.2010. Identification and functional analysis of PCNA1 and PCNA-like1 genes of Phaseolus coccineus. Journal of Experimental Botany,61:873-888
Takasaki Y, Kaneda K, Matsushita M, Yamada H, Nawata M, Matsudaira R, Asano M, Mineki R, Shindo N, Hashimoto H.2004. Glyceraldehyde 3-phosphate dehydrogenase is a novel autoantigen leading autoimmune responses to proliferating cell nuclear antigen multiprotein complexes in lupus patients. International Immunology,16:1295-1304
Tanaka I.1997. Differentiation of generative and vegetative cells in angiosperm pollen. Sex Plant Reprod, 10:1-7
Tank J G, Thaker V S.2011. Cyclin dependent kinases and their role in regulation of plant cell cycle. Biologia Plantarum,55(2):201-212
Taylor P E, Glover J A, Lavithis M, Craig S, Singh M B, Knox R B, Dennis E S, Chaudhury A M.1998. Genetic control of male fertility in Arabidopsis thaliana:structural analyses of postmeiotic developmental mutants. Planta,205:492-505
Tedege M, Kuhlemeier, C.1997. Aerobic fermentation during tobacco pollen development. Plant Molecular Biology,35:3433-3454
Thelen J J, Miernyk J A, Randall D D.1999. Molecular cloning and expression analysis of the mitochondrial pyruvate dehydrogenase from maize. Plant Physiology,119:635-643
Thompson P, Reid E E, Lyttle C R, Dennis D T.1977. Pyruvate dehydrogenase complex from higher plant mitochondria and proplastids:regulation. Plant Physiology,59(5):854-858
Tong W H, Rouault T A.2006. Functions of mitochondrial ISCU and cytosolic ISCU in mammalian iron-sulfur cluster biogenesis and iron homeostasis. Cell Metabolism,3(3):199-210
Tover-Mendez A T, Miernyk J A, Randall D D.2003. Regulation of pyruvate dehydrogenase complex activity in plant cells. European Journal of Biochemistry,270:1043-1049
Twell D, Howden R.1998. Cell polarity, asymmetric division and cell fate determination in developing pollen. In Reproductive Biology. Edited by Owens, S.J. and Rudall, P.J. pp.197-218. Royal Botanic Gardens, Kew, UK.
Uchida K.2003.4-Hydroxy-2-nonenal:a product and mediator of oxidative stress. Prog Lipid Res,42: 318-343
Uetz, P, Giot L, Cagney G, Mansfield T A, Judson R S, Knight J R, Lockshon D, Narayan V, Srinivasan M, Pochart P, Qureshi-Emili A, Li Y, Godwin B, Conover D, Kalbfeisch T, Vijayadamodar G, Yang M J, Johnston M, Fields S, Rothberg J M.2000. A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae. Nature,403:623-627
Vanlerberghe G, Meintosh L.1996. Signals regulating the expression of the nuclear gene encoding altemative oxidase of plant mitoehondria. Plant physiology,111(2):589-595
Vidal M, Brachmann R K, Fattaey A, HarlowE, Boeke J D.I996. Reverse two-hybrid and one-hybrid systems to detect dissociation of protein-protein interactions. Proc Natl Acad Sci U S A, 93:10315-10320
Volker K W, Knull H.1997. A glycolytic enzyme binding domain on tubulin. Archives of Biochemistry Biophysics,338:237-243
Waadt R, Schmidt L K, Lohse M, Hashimoto K, Bock R,Kudla J.2008. Multicolor bimolecular fluorescence complementation reveals simultaneous formation of alternative CBL/CIPK complexes in planta. Plant J,56 (3):505-526
Walter M, Chaban C, Schutze K, Batistic O, Weckermann K, Nake C, Blazevic D, Grefen C, Schumacher K, Oecking C, Harter K, Kudla J.2004. Visualization of protein interactions in living plant cells using bimolecular fluorescence complementation. Plant J,40(3):428-4438
Wang M M, Reed R R.1993. Molecular cloning of the olfactory neuronal transcription factor Olf-1 by genetic selection in yeast. Nature,364(6433):121-126
Weinthal D, Tzfira T.2009. Imaging protein-protein interactions in plant cells by bimolecular fluorescence complementation assay. Trends in Plant Science,14(2):59-62
Wen L, Chase C D.1999. Mitochondrial gene expression in developing male gametophytes of male-fertile and S male-sterile maize. Sex Plant Reproduction,11:323-330
West A H, Stock A M.2001.Histidine kinases and response regulator proteins in two-component signaling systems. Trends Biochem Sci,26(6):369-376
Xie C T, Yang Y H, Qiu Y L, Zhu X Y, Tian H Q.2005. Cytochemical investigation of genetic male-sterility in chinese cabbage. Sex Plant Reproduction,18:75-80
Xu C H, Liu Z T, Zhang L P, Zhao C P, Yuan S H, Zhang F T.2012. Organization of actin cytoskeleton during meiosis I in a wheat thermo-sensitive genic male sterile line. Protoplasma (Published online)
Yamaguchi-Shinozaki K, Shinozaki K.1994. A novelcis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress. Plant Cell,6:251-264
Yan L F, Liu G Q, Xiao X G. 2000. From pollen actin to crop male sterility. Chinese Science Bulletin,45: 784-788
Yui R, Iketani S, Mikami T, Kubo T.2003. Atisense inhibition of mitochondrial pyruvate dehydrogenase El [alpha] subunit in anther tapetum causes male sterility. Plant J,34:57-66
Zhang D S, Liang W Q, Yin C, Zong J, Gu F W, Zhang D B.2010a. OsC6, encoding a lipid transfer protein, is required for postmeiotic anther development in rice. Plant Physiology,154:149-162
Zhang D S, Liang W Q, Yuan Z, Li N, Shi J, Wang J, Liu Y M, Yu W J, Zhang D B.2008. Tapetum degeneration retardation is critical for aliphatic metabolism and gene regulation during rice pollen development. Mol Plant,1(4):599-610
Zhang Q R, Teng H, Sun Y Q, Xiu Z L, Zeng A P.2008. Metabolic flux and robustness analysis of glycerol metabolism in Klebsiella pneumoniae. Bioprocess and Biosystems Engineering,31(2):127-135
Zheng L, Roeder R G, Luo Y.2003. S-phase activation of the histone H2B promoter by OCA-S, a coactivator complex that contains GAPDH as a key component. Cell,114:255-266
Zheng Z, Xia Q, Dauk M, Shen W, Selvaraj G, Zou J.2003. Arabidopsis AtGPAT1, a member of the membrane-bound glycerol-3-phosphate acyltransferase gene family, is essential for tapetum differentiation and male fertility. Plant Cell,15:1872-1887
Zou J T, Qi Q G, Katavic V, Marillia E F, Taylor D C.1999. Effects of antisense repression of an Arabidopsis thaliana pyruvate dehydrogenase kinase cDNA on plant development. Plant Mol Biol,41: 837-849
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