电针不同穴位对肠易激综合征模型大鼠肠道微循环、脑肠相关神经肽影响的研究
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摘要
目的：肠易激综合征(Irritable Bowel Syndrome, IBS)是现代心身性胃肠道疾病,其临床特点复杂,很难用解剖生化等结果解释。对IBS的发病机制目前尚未完全阐明,多数学者认为是各种原因引起的中枢神经、肠神经丛的调节失衡所致,脑长轴的异常导致神经递质的分泌失衡,从而引起肠道一系列症状。针灸可以通过调节脑肠轴功能紊乱进而调节神经递质、神经肽类物质的分泌水平达到治疗的目的,且具有疗效好、副作用少、价格便宜等优势,固本实验选用足三里、天枢穴位进行电针治疗,观察针刺治疗后对IBS模型大鼠的即刻效应,包括内脏敏感性、肠道微循环管径与血流量的变化及血浆中CGRP、ET、VIP水平、结肠中NPY、PGE2、CGRPmRNA、ET受体的变化,初步探讨针刺不同穴位治疗IBS的效应差异及分子生物学机制。
方法：采用Wistar幼鼠为研究对象,以直肠内醋酸刺激法制备肠易激综合征大鼠模型,随机分为正常组、模型组、天枢组、足三里组。模型组、针刺穴位组从第8周开始均采用自制布袋束缚,穴位组进行电针处理1次,正常组不做任何处理。治疗结束后进行(1)内脏敏感性评估(2)采用微循环检测系统观察各组大鼠肠系膜微循环管径、血流速度的变化.(3)并采用酶联免疫法(Elisa)检测大鼠血浆中CGRP、VIP、ET及结肠NPY、 PGE2的水平(4)分别采用Elisa及RT-PCR(?)去检测大鼠结肠组织中CGRPmRNA、ET受体的水平。
结果：
1.内脏敏感性评估：与正常组比较,IBS模型组大鼠背部拱起、腹部抬起值均明显降低(P<0.01),与模型组比较：即刻电针足三里组大鼠腹部抬起值升高(P<0.05)。
2.肠系膜微循环检测：①肠道微血管直径：与正常组比较,模型组肠系膜微血管直径在30分钟时明显变窄(P<0.01)；与模型组相比,即刻电针足三里组在10min、20min、30min不同时间段大鼠肠系膜微血管直径扩张显著(P<0.05,P<0.01)。②肠系膜微循环血流状态,与正常组比较,模型组大鼠肠系膜微循环血流速度明显减慢(P<0.05)；与模型组相比,即刻电针足三里组、天枢组肠系膜微循环血流速度有加快趋势,但未见统计学差异。
3.血浆中CGRP、VIP、ET水平：与正常组比较,模型组大鼠血浆中CGRP含量降低(P<0.05),血浆中ET水平升高,(P<0.05)；即刻电针天枢组大鼠血浆中CGRP水平明显升高(P<0.01)。与模型组比较：即刻电针足三里组、天枢组大鼠血浆中CGRP水平均升高(P<0.05,P<0.01), VIP含量明显降低(P<0.01),ET水平降低(P<0.05)。
4.结肠组织中NPY、PGE2水平：与正常组比较,模型大鼠结肠中NPY、PGE2含量均降低(P<0.05)。与模型组比较：即刻电针天枢组、足三里组NPY、PGE2含量均有明显上升(P<0.01)。
5.结肠组织中CGRPmRNA、ET受体：与正常组比较,模型组、即刻天枢组、即刻足三里组结肠中的CGRPmRNA表达、ET受体均明显增强(P<0.05,P<0.01)。与模型组比较,即刻足三里组结肠中CGRPmRNA表达减弱、ET受体含量减少(P<0.05)。结论：
1.母子分离加幼鼠醋酸灌肠可以建立较稳定的IBS模型大鼠。
2.针刺足三里可以改善IBS模型大鼠的内脏敏感性及肠系膜微循环血流状态。
3.IBS模型大鼠CGRP及其受体、ET及其受体、NPY、PGE2、VIP水平异常、比值失调,可能是导致IBS发生的机制之一。而针刺可以通过调节它们之间的紊乱状态进而达到对IBS的治疗作用。
4.即刻电针介入后,能够调节机体的内环境紊乱和各种脑肠肽的平衡失调。
5.不同穴位具有不同的调节作用,天枢穴对血浆中CGRP调节作用较强,足三里穴无论是在受体和基因表达方面还是在内脏敏感性评估以及缓解微循环障碍方面都表现出明显的作用。
Objective:Irritable Bowel Syndrome(IBS) is a well known functional bowel disorder and a kind of typical Mental and Physical disease. It increased year by year. The pathogenesis is undefined, most of research shows that the pathogenesis of IBS focus on brain-bowel axis disorder and gut feeling high sensitivity. Acupuncture can adjust levels of brain-bowel axis disorder, and acupuncture treatment method is cheap、simple、convenient. So we select Zusanli Point and Tianshu Point to treat IBS model rat by Electric Acupuncture in order to observe the immediate effect, include visceral sensitivity, microcirculation of intestine, bioactive substance in plasma(CGRP、ET、VIP), bioactive substance in colon tissue(NPY、 PGE2、CGRPmRNA、ETR-A). Discuss the part of the mechanism of IBS by acupuncture treatment method and basis of bioactive substance.
Methods:Adopt the Vistar newborn rats as IBS model by continuous rectum acetic acid stimulate, separate for4groups:normal group、model group、Zusanli group、Tianshu group. The electroacupunture are given to Zusanli and Tianshu group once from8th week, after treatment, the rats are accept the visceral sensitivity evaluation with the abdominal retraction reflection(AWR), and observed blood flow of mesentery microcirculation in different time period, after that, phlebotomize5ml abdominal aortic blood and take colon tissue. Check CGRP、ET、VIP in rat's plasma by Enzyme immunoassay method. Check NPY、PGE2、 ETR-A in rat's colon tissue by Enzyme immunoassay method. Check the calcitonin-gene-related peptide (CGRP) content in rat's colon tissue by RT-PCR method.
Results:
1. Estimate on visceral sensitivity of IBS model rats by electroacupuncture:Compared with the normal group, the value of the model group rats'belly raising and back hunch-up significantly reduced (P<0.01). Compared with the model group, the value of the Zusanli group rats'belly raising increased (P<0.05).
2. test of the mesentery microcirculation of IBS model rats:①the diameter of the capillaries:compared with the normal group, the diameter of the mesentery capillaries of the model group significantly narrowed in30mins(P<0.01); Compared to the model group, the diameter of the mesentery capillaries of the Zusanli group significantly expanded (P<0.01).②the state of the blood flow:compared with the normal group, the model group's blood flow speed of mesentery capillaries reduced or stopped (P<0.05).
3. Value of CGRP、VIP、ET in plasma:compared with the normal group, The content of CGRP in plasma of model rats were reduce(P<0.05); The content of CGRP in plasma of Zusanli group were ascent (P<0.01), Compared to the model group, the content of CGRP in plasma of Zusanli、Tianshu group was significantly ascend(P<0.05,P<0.01), while for VIP and ET, Compared to the model group, the content of VIP and ET in plasma was significantly descend (P<0.01,P<0.05) in Zusanli、Tianshu group.
4. Compared with the normal group, the content of NPY、PGE2in colon tissue of model rats were reduce (P<0.05); Compared to the model group, the content of NPY、PGE2in colon tissue of Zusanli、Tianshu group was significantly ascend(P<0.01).
5. Compared with the normal group, the content of CGRPmRNA、ETR-A in colon tissue of model rats were ascent (P<0.05); Compared to the model group, the content of CGRPmRNA、ETR-A in colon tissue of Zusanli group was reduce (P<0.05).
Conclusion:
1. The stable IBS young rat visceral hypersensitivity model can be built by by continuous rectum acetic acid stimulate.
2. Electroacupunture Tianshu、Zusanli point can significantly improve the blood state of the mesentery microcirculation immediately, Zusanli point has stronger effect of pain relief.
3. The content of CGRP、VIP、ET、CGRPmRNA、ETR-A、NPY、PGE2disorder, it may be one of mechanism of IBS.
4. Zusanli、Tianshu Point leads to difference of acupoint effect, Tianshu point has stronger regulation Of CGRP in the plasma, and Zusanli point has more clearly adjustment action for receptor and gene.
方法：采用Wistar幼鼠为研究对象,以直肠内醋酸刺激法制备肠易激综合征大鼠模型,随机分为正常组、模型组、天枢组、足三里组。模型组、针刺穴位组从第8周开始均采用自制布袋束缚,穴位组进行电针处理1次,正常组不做任何处理。治疗结束后进行(1)内脏敏感性评估(2)采用微循环检测系统观察各组大鼠肠系膜微循环管径、血流速度的变化.(3)并采用酶联免疫法(Elisa)检测大鼠血浆中CGRP、VIP、ET及结肠NPY、 PGE2的水平(4)分别采用Elisa及RT-PCR(?)去检测大鼠结肠组织中CGRPmRNA、ET受体的水平。
结果：
1.内脏敏感性评估：与正常组比较,IBS模型组大鼠背部拱起、腹部抬起值均明显降低(P<0.01),与模型组比较：即刻电针足三里组大鼠腹部抬起值升高(P<0.05)。
2.肠系膜微循环检测：①肠道微血管直径：与正常组比较,模型组肠系膜微血管直径在30分钟时明显变窄(P<0.01)；与模型组相比,即刻电针足三里组在10min、20min、30min不同时间段大鼠肠系膜微血管直径扩张显著(P<0.05,P<0.01)。②肠系膜微循环血流状态,与正常组比较,模型组大鼠肠系膜微循环血流速度明显减慢(P<0.05)；与模型组相比,即刻电针足三里组、天枢组肠系膜微循环血流速度有加快趋势,但未见统计学差异。
3.血浆中CGRP、VIP、ET水平：与正常组比较,模型组大鼠血浆中CGRP含量降低(P<0.05),血浆中ET水平升高,(P<0.05)；即刻电针天枢组大鼠血浆中CGRP水平明显升高(P<0.01)。与模型组比较：即刻电针足三里组、天枢组大鼠血浆中CGRP水平均升高(P<0.05,P<0.01), VIP含量明显降低(P<0.01),ET水平降低(P<0.05)。
4.结肠组织中NPY、PGE2水平：与正常组比较,模型大鼠结肠中NPY、PGE2含量均降低(P<0.05)。与模型组比较：即刻电针天枢组、足三里组NPY、PGE2含量均有明显上升(P<0.01)。
5.结肠组织中CGRPmRNA、ET受体：与正常组比较,模型组、即刻天枢组、即刻足三里组结肠中的CGRPmRNA表达、ET受体均明显增强(P<0.05,P<0.01)。与模型组比较,即刻足三里组结肠中CGRPmRNA表达减弱、ET受体含量减少(P<0.05)。结论：
1.母子分离加幼鼠醋酸灌肠可以建立较稳定的IBS模型大鼠。
2.针刺足三里可以改善IBS模型大鼠的内脏敏感性及肠系膜微循环血流状态。
3.IBS模型大鼠CGRP及其受体、ET及其受体、NPY、PGE2、VIP水平异常、比值失调,可能是导致IBS发生的机制之一。而针刺可以通过调节它们之间的紊乱状态进而达到对IBS的治疗作用。
4.即刻电针介入后,能够调节机体的内环境紊乱和各种脑肠肽的平衡失调。
5.不同穴位具有不同的调节作用,天枢穴对血浆中CGRP调节作用较强,足三里穴无论是在受体和基因表达方面还是在内脏敏感性评估以及缓解微循环障碍方面都表现出明显的作用。
Objective:Irritable Bowel Syndrome(IBS) is a well known functional bowel disorder and a kind of typical Mental and Physical disease. It increased year by year. The pathogenesis is undefined, most of research shows that the pathogenesis of IBS focus on brain-bowel axis disorder and gut feeling high sensitivity. Acupuncture can adjust levels of brain-bowel axis disorder, and acupuncture treatment method is cheap、simple、convenient. So we select Zusanli Point and Tianshu Point to treat IBS model rat by Electric Acupuncture in order to observe the immediate effect, include visceral sensitivity, microcirculation of intestine, bioactive substance in plasma(CGRP、ET、VIP), bioactive substance in colon tissue(NPY、 PGE2、CGRPmRNA、ETR-A). Discuss the part of the mechanism of IBS by acupuncture treatment method and basis of bioactive substance.
Methods:Adopt the Vistar newborn rats as IBS model by continuous rectum acetic acid stimulate, separate for4groups:normal group、model group、Zusanli group、Tianshu group. The electroacupunture are given to Zusanli and Tianshu group once from8th week, after treatment, the rats are accept the visceral sensitivity evaluation with the abdominal retraction reflection(AWR), and observed blood flow of mesentery microcirculation in different time period, after that, phlebotomize5ml abdominal aortic blood and take colon tissue. Check CGRP、ET、VIP in rat's plasma by Enzyme immunoassay method. Check NPY、PGE2、 ETR-A in rat's colon tissue by Enzyme immunoassay method. Check the calcitonin-gene-related peptide (CGRP) content in rat's colon tissue by RT-PCR method.
Results:
1. Estimate on visceral sensitivity of IBS model rats by electroacupuncture:Compared with the normal group, the value of the model group rats'belly raising and back hunch-up significantly reduced (P<0.01). Compared with the model group, the value of the Zusanli group rats'belly raising increased (P<0.05).
2. test of the mesentery microcirculation of IBS model rats:①the diameter of the capillaries:compared with the normal group, the diameter of the mesentery capillaries of the model group significantly narrowed in30mins(P<0.01); Compared to the model group, the diameter of the mesentery capillaries of the Zusanli group significantly expanded (P<0.01).②the state of the blood flow:compared with the normal group, the model group's blood flow speed of mesentery capillaries reduced or stopped (P<0.05).
3. Value of CGRP、VIP、ET in plasma:compared with the normal group, The content of CGRP in plasma of model rats were reduce(P<0.05); The content of CGRP in plasma of Zusanli group were ascent (P<0.01), Compared to the model group, the content of CGRP in plasma of Zusanli、Tianshu group was significantly ascend(P<0.05,P<0.01), while for VIP and ET, Compared to the model group, the content of VIP and ET in plasma was significantly descend (P<0.01,P<0.05) in Zusanli、Tianshu group.
4. Compared with the normal group, the content of NPY、PGE2in colon tissue of model rats were reduce (P<0.05); Compared to the model group, the content of NPY、PGE2in colon tissue of Zusanli、Tianshu group was significantly ascend(P<0.01).
5. Compared with the normal group, the content of CGRPmRNA、ETR-A in colon tissue of model rats were ascent (P<0.05); Compared to the model group, the content of CGRPmRNA、ETR-A in colon tissue of Zusanli group was reduce (P<0.05).
Conclusion:
1. The stable IBS young rat visceral hypersensitivity model can be built by by continuous rectum acetic acid stimulate.
2. Electroacupunture Tianshu、Zusanli point can significantly improve the blood state of the mesentery microcirculation immediately, Zusanli point has stronger effect of pain relief.
3. The content of CGRP、VIP、ET、CGRPmRNA、ETR-A、NPY、PGE2disorder, it may be one of mechanism of IBS.
4. Zusanli、Tianshu Point leads to difference of acupoint effect, Tianshu point has stronger regulation Of CGRP in the plasma, and Zusanli point has more clearly adjustment action for receptor and gene.
引文
[1]张声生,李乾构,魏玮,来要良.肠易激综合征中医诊疗共识意见.中华中医药杂志2010,25：1062-1065.
[2]Longstreth GF, Thompson WG, Chey WD, et al. Functional bowel disorders. Gastroenterology,2006,130:1480-1419
[3]Harris LA,Hansel S,DiBaise J,et al.Irritable bowel syndrome and chronic constipation:emerging drugs,devices,and surgical treatments.Curr Gastroenterol Rep,2006,8:282-290.
[4]Spinelli A. Irritable bowel syndrome. Clin Drug In-vestig 2007,27:15-333 Xiong LS, Chen MH, Chen HX, Xu AG, Wang WA,Hu PJ. A population-based epidemiologic study of irritable bowel syndrome in South China:
[5]潘国宗,鲁素彩,柯美云,等.北京地区肠易激综合征的流行病学研究：一个整群、分层、随机的调查.中华流行病学杂志,2000,21：26-29.
[6]熊理守,陈旻湖,陈惠新,等.广东省社区人群肠易激综合征的流行病学研究.中华医学杂志,2004,84：278-281.
[7]Wingate D,Hongo M, Kellow J, et al.Disorders of gastrointestinal motility:towards a new classification.J Gastroenterol Hepatol,2002,17(Suppl):S 1-14.
[8]Andresen V, Camilleri M. Irritable bowel syndrome:recent and novel therapeutic approaches. Drugs 2006,66:1073-1088
[9]O'Mahony SM, Hyland NP, Dinan TG, Cryan JF. Maternal separation as a model of brain-gut axis dysfunction. Psychopharmacology (Berl)2010 Oct 1 [Epub ahead of print] PMID:20886335
[10]Sheehan J, Gaman A, Vangel M, Kuo B. Pooled analysis of brain activity in irritable bowel syndrome and controls during rectal balloon distension. Neurogastroenterol Motil 2010 Nov 30. doi:10.1111/j.1365-2982.2010.01635.x.
[11]Elsenbruch S. Abdominal pain in irritable bowel syndrome:a review of putative psychological, neural and neuro-immune mechanisms. Brain Behav Immun 2010 Nov 20.
[12]Ohman L,Simren M. Pathogenesis of IBS:role of inflammation, immunity and neuroimmune interactions. Nat Rev Gastroenterol Hepatol 2010,7:163-73. Epub 2010 Jan 26.
[13]Park MI,Camilleri M.Is there a role of food allergy in irritable bowel syndrome and functional dyspepsia? A systematic review.Neurogastroenterol Motil,2006,18:595-607.
[14]Chang L,Mayer EA,Labus JS,et al. Effect of sex on perception of rectosigmoid stimuli in irritable bowel syndrome.Am J Physiol Regul Integr Comp Physiol,2006,291:R277-284.
[15]Houghton LA,Jackson NA,Whorwell PJ,et al.Do male sex hormones protect from irritable bowel syndrome? Am J Gastroenterol,2000,95:2296-2300.
[16]Heitkemper MM,Jarrett ME.Update on irritable bowel syndrome and gender differences.Nutr Clin Pract,2008,23:275-283.
[17]Ruigomez A,Garcia Rodriguez LA,Johansson S,et al.Is hormone replacement therapy associated with an increased risk of irritable bowel syndrome? Maturitas,2003,44:133-140.
[18]Naliboff BD,Berman S,Chang L,et al.Sex-related differences in IBS patients:central processing of visceral stimuli.Gastroenterology,2003,124:1738-1747.
[19]Smith JL,Bayles D.Postinfectious irritable bowel syndrome:a longterm consequence of bacterial gastroenteritis.J Food Prot,2007,70:1762-1769.
[20]De Giorgio R,Barbara G.Is irritable bowel syndrome an inflammatory disorder? Curr Gastroenterol Rep,2008,10:385-390.
[21]Spiler R,Campbell E.Post-infectious irritable bowel syndrome[J].Curropin Gastroenterol, 2007,9(5):378-384.
[22]O'Mahony L,McCarthy J,Kelly P,et al.Lactobacillus and bifidobacterium in irritable bowel syndrome:symptom responses and relationship to cytokine profiles.Gastroenterology, 2005,128:541-551.
[23]Lin H.Small intestinal bacterial overgrowth:a framework for understanding irritable bowel syndrome.JAMA,2004,292:852-858.
[24]张茹.胃肠激素与肠易激综合征[J].标记免疫分析与临床,2004,11(1)：44-46.
[25]刘丽宏,梅其炳,陈蕾等.5-羟色胺对培养胃底平滑肌细胞内游离钙动员的影响[J].第四军医大学学报,2001,22(10)：891.
[26]Chey WY,Jin HO,Lee MH,et al.Colonic motility abnormality in patients with irritable bowel syndrome exhibiting abdominal pain and diarrhea.Am J Gastroenterol,2001,96:1499-1506.
[27]Sagami Y,Hongo M.The gastrointestinal motor function in irritable bowel syndrome(IBS).Nippon Rinsho,2006,64:1441-1445.
[28]Sagami Y,Shimada Y,Tayama J,et al.Effect of a corticotropin releasing hormone receptor antagonist on colonic sensory and motor function in patients with irritable bowel syndrome.Gut,2004,53:958-964.
[29]Kalantar JS, Locke GR, Zinsmeister AR, et al. Familial agg-regation of irritable bowel syndrome:a prospective study[J]. Gut,2003,52:1703-1707.
[30]Kanazawa M,Endo Y,Whitehead WE,et al.Patients and nonconsulters with irritable bowel syndrome reporting a parental history of bowel problems have more impaired psychological distress.Dig Dis Sci,2004,49:1046-1053.
[31]Kim HJ, Camilleri M, Carlson PJ, et al. Association of distinct alpha(2) adrenoceptor and serotonin transporter polymorphisms with constipation and somatic symptoms in functional gastrointestinal disorders[J]. Gut,2004,53:829-837.
[32]Gonsalkorale WM, Perrey C, Pravica V, et al. InterleukinlO genotypes in irritable bowel syndrome:evidence for an inflammatory component[J],Gut,2003,52:91-93.
[33]罗小雨,钟良.肠易激综合征发病机制的研究进展[J],国际消化病杂志,2010,30(6)：321-324.
[34]Hayee B,Forgacs I.Psychological approach to managing irritable bowel syndrome.BMJ,2007,334:1105-1109.
[35]Endo Y.Psychological symptoms in IBS.Nippon Rinsho,2006,64:1471-1476.
[36]Hagymasi K,Tulassay Z.Genetic background of irritable bowel syndrome.Orv Hetil, 2006,147:1167-1170.
[37]Bouin M,Plourde V,Boivin M,et al.Rectal distention testing in patients with irritable bowel syndrome:sensitivity,specificity,andpredictive values of pain sensory thresholds.Gastroenterology,2002,122:1771-1777.
[38]Mayer EA.The neurobiology of stress and gastrointestinal disease.Gut,2000,47:861-869.
[39]Poitras P,Riberdy Poitras M,Plourde V,et al. Evolution of visceral sensitivity in patients with irritable bowel syndrome[J].Dig Dis Sci,2002,47(4):914-920.
[40]Schwartz MP, Samsom M, Smout AJ. Chemospecific alterations in duodenal perception and motor response in functional dyspepsia. Am J Gastroenterol 2001,96(9):2596-2602
[41]Kaneko H,Imamura H,Konagaya T.Visceral hypersensitivity.Nippon Rinsho,2006,64:1446-1451.
[42]Posserud I,Agerforz P,Ekman R,et al.Altered visceral perceptual and neuroendocrine response in patients with irritable bowel syndrome during mental stress.Gut,2004,53:1102-1108.
[43]Dickhaus B,Mayer EA,Firooz N,et al. Effect of auditory stress on perceptual and emotional ratings of visceral stimuli in IBS patients. Am J Gastroenterol,2003,98:135-143.
[44]胡品津.从脑-肠互动的高度认识肠易激综合征[J].中华消化杂志,2003,23(5)：261.
[45]Talley NJ,Howell S,Poulton R.The irritable bowel syndrome and psychiatric disorders in thecommunty:is there a link[J].Am J gastroenterol,2001,96(4):1072.
[46]Mayer EA,Berman S,Suyenobu B,et al.Differences in brain responses to visceral pain between patients with irritable bowel syndrome and ulcerative colitis.Pain,2005,115:398-409.
[47]Yuan YZ,Tao RJ,et al.Functional brain imaging in irritable bowel syndrome with rectal balloon-distention by using MRI.World J Gastroenterol,2003,9(6):1356.
[48]Chang L,Berman S,Mayer EA,et al.Brain responses to visceral and somatic stimuli in patients with irritable bowel syndrome with and without fibromyalgia.Am J Gastroenterol,2003,98:1354-1361.
[49]Wilder-Smith CH,Schindler D,Lovblad K,et al.Brain functional magnetic resonance imaging of rectal pain and activation of endogenous inhibitory mechanisms in irritable bowel syndrome patient subgroups and healthy controls.Gut,2004,53:1595-1601.
[50]Longstreth GF,ThbmPson WG,Chey WD,et al. Functional bowel disorders.Gastroe-nterology,2006,130:1480-1491.
[51]O'Donnell LJ,Virjee J,Heaton KW.Detection of pseudodiarrhoea by simple clinical assessment of intestinal transit rate.BMJ,1990,300:439-440.
[52]张琳,周孝德,赵国福,等.肠易激综合征中西医研究进展[J].甘肃中医.2003,16(2)：46-48.
[53]王永炎.中医内科学.上海科学技术出版社.1997：172-201.
[54]周福生,吴文江,黄志新.顺激合剂治疗肠易激综合征疗效观察[J].广州中医药大学学报.2002,19(4)：269-271.
[55]胡旭光,张思为.疏肝、健脾、疏肝健脾方药对肠易激综合征模型大鼠的治疗作用及机理探讨[J].河南中医,2007,27(9)：23-24.
[56]杨银芳,楚更五,张建.痛泻要方对寒冷-束缚肠易激综合征模型大鼠作用的实验研究[J].中华中医药学刊,2008,26(9)：1984-1986.
[57]朱力阳,彭成,彭延娟.四逆散治疗大鼠肝脾不调证肠易激综合征模型的研究.四川中医[J],2010,28(8)：10-11.
[58]李浩,孙建华.针灸治疗肠易激综合征临床研究概况及机制探讨.中国中医急症[J],2011,20(3)：430-431.
[59]骆燕宁,孙彩霞.针灸治疗结肠易激症群158例[J].上海针灸.2003,22(4)：28.
[60]段彩琴,李爱萍.温针治疗肠易激综合征疗效观察[J].中国针灸.2001,21(4)：226.
[61]王美娟.针刺夹脊穴治疗肠易激综合征32例[J].湖南中医药导报.2002,8(5)：272-273.
[62]安广青,李娜,翟国华,等.针灸治疗肠易激综合征疗效评价[J].上海针灸杂志.2010,29(6)：354-356.
[63]何严,高宏.针灸配合按摩治疗肠易激综合征36例[J].陕西中医.2004,25(3)：258.
[64]王伟.温针治疗肠易激综合征腹泻型临床观察[J].中国中医急症.2008,17(10)：1377-1407.
[65]吴向琼.温针并耳压治疗肠易激综合征46例临床观察[J].湖南中医药导报.2004,10(3)：42.
[66]高军,韩景献.针刺治疗肠易激综合征32例.针灸临床杂志[J],2010,26(9)：24-25.
[67]王文文.穴位埋线法治疗肠易激综合征33例体会[J].现代中西医结合杂志.2002,11(4)：328-329.
[68]丁淑强,李大军.承山穴为主治疗肠易激综合征54例疗效观察[J].针灸临床杂志,2004,20(5)：53.
[69]刘立功,顾杰.急病针灸典籍通览[M].上海：上海科学技术出版社,2000.
[70]黄琴峰.针灸治疗便秘临床规律探讨.辽宁中医杂志,2009,36(3)：368-369.
[71]高巍,黄裕新,张洪新,等.电针“足三里”对大鼠脑肠肽含量的影响及其对免疫系统的调控作用.针刺研究[J].2002,27(1)：50-54.
[72]常小荣,严洁,刘玉群,等.电针足三里和阳陵泉穴对家兔胃胆运动及脑肠肤的影响.世界华人消化杂志.2006,14(17)：1662-1668
[73]吴亚丽,陈少夫,潘莉莉.电针对胃炎患者胃酸分泌及胃肠激素的影响[J].中国中西医结合杂志,1994,14(12)：709.
[74]Sun DY,Huang YX,Cu ZH,Gao W,Wang QL.Effects of electroacupuncture on the pressure in pylonic antrum and brain-gut peptide content in dogs.Shijie Huaren Xiaohua Zazhi,1999;7:997-999.
[75]Huang YX, Sun DY, Chu ZH,Gao W, Wang QL. Effect of electroacupunture on canine pyloric pressure and its relation to somatostatin and nitric oxide. Shijie Zhenjiu Zazhi, 1999,9:43-46.
[1]Spiegel BM, Gralnek IM, Bolus R, Chang L, Dulai GS, Mayer EA, Naliboff B. Clinical determinants of health-related quality of life in patients with irritable bowel syndrome[J]. Arch Intern Med 2004,164:1773-1780.
[2]胡品津.从脑-肠互动的高度认识肠易激综合征[J].中华消化杂志,2003,23(5)：261-262.
[3]Talley NJ,Howell S,Poulton R.The irritable bowel syndrome and psychiatric disorders in thecommunty:is there a link? [J] Am J gastroenterol,2001,96(4):1072.
[4]Hayee B,Forgacs I.Psychological approach to managing irritable bowel syndrome.BMJ,2007,334:1105-1109.
[5]吴晓亮,孙建华.肠易激综合征国内实验研究现状的调查[J].世界华人消化杂志,2010,18(35)：3818-3823.
[6]Gaman A, Kuo B. A review of CNS activation patterns from fMRI studies in IBS patients versus healthy controls. Gastroenterology 2007;32(4 supplement 2):A-602.
[7]Berman S,Munakata J,Naliboff BD,et al.Gender differencesin regional brain response to visceral pressure in IBS patients [J].Eur J Pain,2000,4(2):157-172.
[8]Bonaz B,Baciu M,Papillon E,et al.Central processing of rectal pain in patients with irritable bowel syndrome:an fMRI study[J].Am J Gastroenterol,2002,97(3):654-661.
[9]Accarino AM,Azpiroz F,Malagelada JR.Attention and distraction:effects on gut perception[J]. Gastroenterology,1997,113(2):415-422.
[10]Smith JL,Bayles D.Postinfectious irritable bowel syndrome:a longterm consequence of bacterial gastroenteritis.J Food Prot,2007,70:1762-1769.
[11]De Giorgio R,Barbara G.Is irritable bowel syndrome an inflammatory disorder?Curr Gastroenterol Rep,2008,10:385-390.
[12]朱力阳,彭成,谢晓芳,等.化学刺激引起的两种大鼠内脏高敏感性模型的建立[J].中国药理学通报,2010；26：267-270.
[13]RINGEL Y,DROSSMAN D A, TURKINGTON T G, et al. Dysfunctions of the mofivational affective pain System in patients with IBS:PET brain imaging in response to rectal ballon distention[J]. Gastronterology,2000, (Mav Suppl):A474.
[14]Mayer EA,Berman S,Suyenobu B,et al.Differences in brain responses to visceral pain between patients with irritable bowel syndrome and ulcerative colitis.Pain,2005, 115:398-409.
[15]Ringel Y,Prossman DA,Tukington TG,et al.Dysfunction of the motivational-affective pain system in patients with IBS:PET brain imaging in response to rectal bulloon distension.Gastroenterology,2000,9 (4Suppl):410
[16]Wilder-Smith CH,Schindler D,Lovblad K,et al.Brain functional magnetic resonance imaging of rectal pain and activation of endogenous inhibitory mechanisms in irritable bowel syndrome patient subgroups and healthy controls. Gut,2004,53:1595-1601.
[17]李兆申.肠易激综合征内脏高敏感性研究现状和展望.第二军医大学学报.2003,24(2)：120-123
[18]Gao J,Wu X,Owyang C,et al.Enhanced responses of the anterior Cingulated cortex neurones to colonic distension in viscerally hypersensitive rats[J].J Physiol,2006,570 (Pt-1):169-183.
[19]袁耀宗,陶然君,许斌,等.肠易激综合征患者直肠气囊扩张时的功能脑显像研究[J].Chinese Journal of Gastroenterol.2003,8(supplement):4
[20]Casey KL,Minoshima S,Berger KL,et al.Positron emission tomographic analysis of cerebral structures activated specifically by repetitive noxious heat stimuli[J].J Neurosurg, 1994,71(2):802-807.
[21]Derbyshire SW,Jones AK,Devani P,et al.Cerebral responses to pain in patients with atypical facial pain measured by positron emission tomography[J].J Neurol Neu-rosurg Psychiatry,1994,57(10):1166-1172
[22]Bouin M, et al. Rectal distention testing in patients with irritable bowel syndrome: sensitivity, specificity, and predictive values of pain sensory thresholds. Gastroenterology 2002,122(7):1771-1777
[23]Mertz H, et al. Altered rectal perception is a biological marker of patients with irritable bowel syndrome. Gastroenterology,1995,109(1):40-52
[24]Cervero F, Janig W. Visceral nociceptors:a new world order? Trends Neurosci.1992, 15(10):374-378
[25]De Ponti F·Pharmacology of serotonin:what a clinician should know[J].Gut,2004, 53(10):1520
[26]Pan H,Gershon MD · Activation of intrinsic afferent pathways in submucosal ganglia of the guinea pig small intestine[J].J Neurosci,2000,20:3295
[27]Poitras P,Riberdy Poitras M,Plourde V,et al. Evolution of visceral sensitivity in patients with irritable bowel syndrome[J].Dig Dis Sci,2002,47(4):914-920.
[28]Sinhamahapatra P,Saha SP,Chowdhury A, Chakrabarti SK,Ghosh A,Maiti B.Visceral afferent hypersensitivity in irritable bowel syndrome-evaluation by cerebral evoked potential after rectal stimulation.Am J Gastroenterol 2001; 96:2150-2157
[29]Malcolm A,Phillips SF,Kellow JE,Cousins MJ.Direct clinical evidence for spinal hyperalgesia in a patient with irritable bowel syndrome.Am J Gastroenterol 2001; 96:2427-2431
[30]Fukudo S, Nomura T, Muranaka M,et al. Brain-gut response to stress and cholinergic stimulation in irritable bowel syndrome. A preliminary study [J]. J Clin Gastroentero,l 1993, 17(2):133-141.
[31]董文珠,邹多武,李兆申,许国铭等.肠易激综合征患者内脏高敏性的机制研究.中华消化杂志2004；24：18-22
[32]李延青.什么导致了肠易激综合征.临床消化病杂志.2009,21(2)：110-111
[33]Elsenbruch S, OrrWC. Diarrhea and constipation-predominant IBS patients differ in postprandial autonomic and cortisol responses[J].Am J Gastroentero,l 2001,96(2):460.
[34]孙燕,刘锋霖,宋耿青,等.急性和慢性束缚应激对大鼠内脏敏感性和神经内分泌的影响[J].中华消化杂志,2006,26(1)：38
[35]Talley NJ,Fett SL,Zinsinerster AR,et al·Gastrointestinal tract symptoms and self-reported abuse:a population-based study[J]. Gastroenterology,1994,107(9):1040
[36]Goyal RK, Hirano I. The enteric nervous system[J]. N Engl J Med,1996,334(17): 1106-1115
[37]Basilisco G. Pathogenesis of irritable bowel syndrome:current under-standing [J]. Recenti Prog Med,2007,98(11):543-547
[38]Lucas A, Cobelens PM, Kavelaars A, et al. Disturbed in vitro adren-ergic modulation of cytokine production in inflammatory bowel diseases in remission [J]. Neuroimmunol, 2007,182(1-2):195-203
[39]雷景根.盐酸帕罗西汀治疗肠易激综合征34例临床观察[J].浙江预防医学.2006,18(8)：66-67.
[40]谢启文.神经肽[M].上海：复旦大学出版社,2004,167-169.
[41]林琳,赵志泉,殷咏梅,等.胃肠激素与肠易激综合征的相关性[J].江苏医药杂志,2001,27(3)：166-168.
[42]林琳,赵志泉,殷咏梅,等.胃肠激素与肠易激综合征的相关性[J].江苏医药杂志,2001,27(3)：166-168.
[43]Husum H,Van Kammen D,Termeer E, et al.Neuropsychopharmacology,2003,9:422-428.
[44]Chiocchetti R,PooleDP, KimuraH,et al.Cell Tissue Res,2003,311:11-22
[45]Michalkiewicz M,Zhao G, Jia Z,et al. Central neuropeptide Y signaling ameliorates N(omega)-nitro-L-arginine methyl ester hypertension in the rat through a Y1 receptor mechanism[J].Hypertension,2005,45(4):780-785.
[46]Michalkiewicz M,Knestaut KM,Bytchkova EY,et al.Hypotension and reduced catecholamines in neuropeptide Y transgenic rats[J]. Hypertension,2003,41(5):1056-1062.
[47]Kokare DM,Dandekar MP,Chopde CT,Subhedar N.Interaction between neuropeptide Y and alpha-melanocyte stimulating hormone in amygdale regulates anxiety in rats.Brain Res.2005 May 10;1043(1-2):107-14
[48]李继锋,王舟,纪祥瑞.降钙素基因相关肽在神经免疫内分泌网络中的作用[J].中国神经免疫学和神经病学杂志,2004,1(3)：177-179.
[49]陈军,李静.头针治疗偏头痛的疗效及对CGRP、NO的调节[J].上海针灸杂志,2008,27(12)：6-7.
[50]刘海涛,彭旭,吴曙粤.血浆内皮素及降钙素基因相关肽的研究进展[J],内科,2006,1(2)：173-174.
[51]郑雪雁.实验性慢性结肠炎症大鼠的内脏感觉变化及帕罗西汀的影响[D].福建医科大学,2007：27-28.
[52]张峰,袁川评,柳巨雄.SP和CGRP在炎症性肠病大鼠结肠中的表达规律[J].中国比较医学杂志,2011,21(12)：26-30.
[53]陈吉,邓展生,张阳德.肠易激综合患者结肠黏膜VIP.CGRP和肥大细胞变化及相关性研究[J],中国现代医学杂志,2011,21(8)：934-937.
[54]Parlapiano C, Paoletti V, Campana E, et al. CGRP and ET-1 plasma levels in normal subjects[J].Eur Rev Med Pharmacol Sci,1999;3(3):139-141.
[55]Keith IM. The role of endogenous lung neuropeptides in regulation of the pulmonary circulation[J].Physiol Res,2000;49(5):519-537.
[56]Wang Hai-yun, Shi Jun-jie. Effect of Xiaoyaosan on the ET Levels in Serum of Stressed IBS Rats[J]. Chinese Archives of Traditional Chinese Medicine,2009,27(11):2406-2408
[57]杨磊,赵雅芳,李滢.电针不同穴位对肠易激综合征模型大鼠血浆CGRP、VIP、ET含量的影响[J]吉林中医药,2011,31(12)：1235-1237.
[58]陈言言,王立,董宏生.肠易激综合征及溃疡型结肠炎患者胃肠激素和细胞因子的变化[J].中国中西医结合消化杂志,2003,11(6)：370-371.
[59]Van Geldre LA,Lefebvre RA.Interaction of NO and VIPin gastrointestinal smooth muscle relaxation[J].CurrPharm Des,2004,10(20):2483-2497.
[60]Evangelista S.Involvement of tachykinins in intestinal inflammation[J].Curr Pharm Des,2001,7(1):19-30.
[61]Wu HG,Jiang B,Zhou EH,et al.Regulatory Mechanism of Electro-acupuncture in Irritable Bowel Syndrome:Preventing MC Activation and Decreasing SP VIP Secretion[J].Dig Dis Sci,2007,13
[62]李兆申,董文珠,邹多武,等.肠易激综合征肠黏膜SP、VIP、CGRP变化的研究[J],第二军医大学学报,2003,24(2)：147-150.
[63]梁荣新,蔡联英,郑琴芳.肠易激综合征患者乙状结肠动力及胃肠激素变化的研究[J].广西医科大学学报,2003,20(6)：843-845.
[64]林琳,赵志泉,殷咏梅,等.胃肠激素与肠易激综合征的相关性[J].江苏医药,2001,27(3)：166-8.
[65]张军,张振玉,孙丹莉.肠易激综合征患者血浆胃肠激素含量的变化,诊断学理论与实践,2006,5(1)：58-59.
[66]陈敏.TNF-α及PGE2与小儿全身炎症反应综合征[J].内蒙古医学杂志Inner MongoliaMed J,2012,44(10),1209-1210.
[67]应延风,陈浩浩,金耀建,等.PGE2对气道平滑肌细胞分泌和凋亡影响的研究[J／CD].中华哮喘杂志：2012,6(4)：239-244.
[68]秦明,黄裕新,王景杰,等.电针在应激大鼠胃粘膜保护作用中对氧自由基和前列腺素的影响[J].第四军医大学学报(J FourthM ilM ed U niv),2001; 22 (9):803-805
[69]L i T, Zhang XJ. The injurious effect of oxygen radicals on the membrance of gastric muco sal cells[J].Beijing Yike Daxue Xuebao (J B eijing Med Univ),1993,25(5):321-323.
[70]Pavies GR,SimmondsNJ,Stevens TR, Granison A, Black DR,Ramp ton DS. M uco sal reactive oxygen metabo lite p roduction in duodenal ulcer disease [J]. Gut,1992; 33 (12): 1467-1472.
[71]L i T, Zhang XJ. Role of oxygen2derived free radicals in stress induced gastric ulceration [J]. Sheng li Xuebao (A cta P hy siolog ica S inica),1993; 45 (3):286-291.
[72]Dong XY, Sen ZS. A p relim inary study on themechanism of resistance of gastric muco sa to the second injury in the rat [J].Beijing Yike Daxue Xuebao (J B eijing Med Univ), 1995; 27(1):9-11.
[73]Konturek PK, Brzozow sk i T, Konturek S, Dembinsk iA. Ro leofepidermal growth facto r, prostaglandin, and sulfhydrylsin stress2induced gastric lesions [J]. Gastroenterology, 1990; 99:1607-1615.
[74]L i T, Zhang XJ. The relationsh ip between sulfhydryls and the protective effect of p rostaglandin E2 and somatostatin on gastricmucosa [J]. B eijing YikeDaxue Xuebao (J Beijing Med Univ),1989; 21 (6):476-478.
[75]Zhao BX, Guo CJ, Yin KJ. Effects of acupuncture on oxygen free radicals andultrastructure in stress rats [J]. Shanghai Zhenjiu Zazhi (JS hanghai Acupunct),1995; 14: 235-236.
[76]Fosslien E. Adverse effects of nonsteroidalanti inflammatory drugs on the gastro intestinal system [J]. Ann Clin Lab Sci,1998:28 (1):67-81.
[77]Sun B, L i ZS. N SA ID induced gastric muco sal damage and its prevention in rats [J]. Shijie Huaren Xiaohua Zazhi (World Chin J Digestol),2000; 8 (3):273-275.
[78]A i BW, Guo CJ, Yin KJ. The effects of acupuncture on prostaglandin in the gastric mucosa in experimeatal gastric ulcerrats [J]. S hanghai Zhenjiu Zaz i (J S hanghai Acupunct),1995; 14:235-236.
[79]蓝升红,周丽萍,屠金夫,等.前列腺素E2对重症胰腺炎大鼠血清肿瘤坏死因子及白介-6的调控作用[J.肝胆胰外科杂志,2001,13(2)：67-69.
[80]薛瑞,苗一非,杨吉春,等.前列腺E2对免疫细胞及炎症相关疾病的调控作用[J].生理科学进展,2011,42(3)：165-168.
[81]邱春兰,王兴勇.全身炎症反应综合征发病机制研究进展[J].西部医学,2009,21(4)：662-663.
[1]陈文杰,田牛主编.微循环的理论与应用[M].北京：人民卫生出版社,1987：23.
[2]田牛.微循环学[M].北京：原子能出版社,2004：40.
[3]于金萍,冷贵生,郭述苏,等.脑梗塞患者红细胞膜微粘度与球结膜微循环之关系[J].微循环学杂志,1996,6(1)：38.
[4]陈邦国.针刺治疗脑血栓形成的微循环观察[J],上海针灸杂志,1997,16(2)：13.
[5]周杰芳,靳瑞.针刺对中风患者微循环与血液流变学作用的观察,广州中医学院学报,1991,8(2)：196.
[6]吴焕金,高镇五,张琳珊.隔药饼灸对慢性非特异性结肠炎患者球结膜微循环的影响[J].针灸学报,1991,(3)：4-6.
[7]李湘.运动性头针对糖尿病视网膜病变患者眼部血流动力学的影响[J].山东医药,2006,46(16)：43-44.
[8]唐照亮.艾灸对血瘀证活血化瘀作用的研讨[J].安徽中医学院学报,2007,26(1)：21-24.
[9]赵雪梅,刘育英,等.100例健康人不同部位体表血流量的测量[J].微循环学杂志,2004,14(2)：43-45.
[10]张栋,王淑友,等.激光多普勒成像技术对针灸效果的观察[J].上海针灸杂志,2004,23(5)：37-40.
[11]张栋,马慧敏,等.激光多普勒成像技术对体表不同部位微血流图像的初步观察[J].中国微循环,2004,8(5)：283-285.
[12]李丽,龚剑秋,丁光宏,等.头排针治疗脑梗死过程中颈动脉血液动力学能量变化研究[J].针刺研究,2009,34(5)：334-338.
[13]胡青华,庄逢源.荧光显微镜观察电针对实验性急性出血性低血压脑微循环的作用[J].微循环学杂志,2000,10(1)：18-20.
[14]韩冰冰,王世军,蒋继强.电针对MC AO大鼠海马微循环血流量的影响[J].时珍国医国药,2010,10,21(3)：730-731.
[15]喻斌,孙永,吕高红,等.电针配合贯叶连翘提取物对抑郁大鼠行为学及脑微循环的影响[J].针刺研究,2011,36(3)：164-170.
[16]卢岩,王世军.电针对局灶性脑缺血大鼠脑葡萄糖代谢的影响[J.]辽宁中医杂志,2011,38(5)：995-999.
[17]马莎,陈祥芳,等.针刺对偏头痛大鼠硬脑膜微循环的影响[J].吉林中医药,2008,28(8)：610-611.
[18]Komori M, Takada K, Tomizawa Y, et, Microcirculatory responses to acupuncture stimulation and phototherapy[J]. Anesth nalg,2009,108(2):635-640.
[19]陈邦国.针刺治疗脑血栓形成的微循环观察[J].上海针灸杂志,1997,16(2)：13.
[20]李信明,魏安宁,李定明.“三联疗法”治疗周围性面神经麻痹及其对面部微循环血流量的影响[J].中华物理医学与康复杂志,2006,28(8)：549-550.
[21]刘雅静,张伯儒.缪刺结合刺络拔罐法治疗顽固性面神经麻痹临床研究[J].吉林中医药,2008,28(1)：46-47.
[22]李明珍,吴人照.电针强刺激对小白鼠耳廓微循环的影响[J].湖北中医杂志,1989(6)：43-44.
[23]阴怀清,王阿琚,范庆,等.脑性瘫痪患儿头针治疗前后耳廓微循环变化的观察[J].山西医药杂志,1992,21(6)：352.
[24]郑重,宋开源,胡晓梅,等.电针改善VBI所致内耳微循环障碍及对VOR的影响[J].中国应用生理学杂志,2006,22(1)：99-104.
[25]刘俊岭,曹庆淑,罗明富,等.电针心包经经穴改善急性心肌缺血机理的研究[J].针刺研究,1999,24(4)：282-287.
[26]罗明富,曹庆淑,刘俊岭,等.电针“内关”穴对急性心肌缺血区微血管作用的透射电镜观察[J].针刺研究,1995,21(4)：11-14.
[27]罗明富,王平,刘俊岭,等.电针“内关”穴对急性心肌缺血区肌原纤维、线粒体及血小板的影响[J].针刺研究,2001,26(2)：119-121.
[28]李洁.电针对实验性急性心肌缺血影响的研究[J].上海针灸杂志,1999,18(2)：44-47.
[29]Bhatia M,Neoptolemos JP,Slacin J.Inflammatory mediators as therapeutic targets in acute pancreatitis.Curr Opin Invest Drugs,2001,2(4):496-501.
[30]陈友岱,周总光.缓激肽在器官微循环损伤中的作用[J].微循环学杂志,2001,11(1)：43-46.
[31]王强,闫军.低相对分子质量肝素改善急性胰腺炎微循环的研究[J].中国药物与临床,2011,11(3)：294-295.
[32]吴富东,王世军,王晓燕,等.电针足三里穴对正常大鼠微循环的调节作用及穴位脏腑相关性研究[J].中医杂志,2010,51(12)：1101-1103.
[33]胡森,王磊,周洁平.电针足三里对烫伤休克大鼠肠黏膜血流和微血管通透性的影响[J]微循环学杂志,2010,20(1)：10-12.
[34]赵雅芳,李春华,嵇╲波,等.电针三阴交、血海对痛经模型大鼠子宫微循环的影响[J].微循环学杂志,2011,21(2)：4-7.
[35]李春华,嵇波,赵雅芳,等.电针预先介入对痛经模型大鼠子宫微循环的影响[J].中华中医药杂志,2010,25(12)：1979-1984.
[36]杨磊,李滢,赵雅芳,等.电针不同穴位对肠易激综合征模型大鼠内脏敏感性及肠系膜微循环的影响[J].微循环学杂志,2011,21(4)：4-6.
[37]康新,卓鹰.电针不同穴位对家兔微循环障碍影响的初步研究[J].中国血液流变杂志,2007,17(3)：491-492.
[38]王诚宏.腰部银质针针刺与下肢微循环变化的研究[J].中国疗养医学,2004,13(3)：134-135.
[39]白良川,许冠荪,张荣军,等.电针对兔激素性股骨头坏死局部组织血流量的影响[J].安徽中医学院学报,2004,23(1)：33-34.
[40]朱柏君,王援朝,徐文斌.电针对急性实验性关节炎大鼠外周微循环的影响[J].针刺研究,1993,18(3)：219-222.
[41]陈军,李静.头针治疗偏头痛的疗效及对CGRP. NO的调节[J].上海针灸杂志,2008,27(12)：6-7.
[42]刘海涛,彭旭,吴曙粤.血浆内皮素及降钙素基因相关肽的研究进展[J],内科,2006,1(2)：173-174.
[43]Parlapiano C, Paoletti V, Campana E, et al. CGRP and ET-1 plasma levels in normal subjects[J].Eur Rev Med Pharmacol Sci,1999;3(3):139-141.
[44]Keith IM. The role of endogenous lung neuropeptides in regulation of the pulmonary circulation[J].Physiol Res,2000;49(5):519-537.
[45]杨磊,赵雅芳,李滢.电针不同穴位对肠易激综合征模型大鼠血浆CGRP、VIP、ET含量的影响[J].吉林中医药,2011,31(12)：1235-1237.
[46]陈言言,王立,董宏生.肠易激综合征及溃疡型结肠炎患者胃肠激素和细胞因子的变化[J].中国中西医结合消化杂志,2003,11(6)：370-371.
[47]Van Geldre LA,Lefebvre RA. Interaction of NO and VIPin gastrointestinal smooth muscle relaxation[J].CurrPharm Des,2004,10(20):2483-2497.
[48]Evangelista S.Involvement of tachykinins in intestinal inflammation[J].Curr Pharm Des,2001,7(1):19-30.
[49]Wu HG, Jiang B, Zhou EH, et al Regulatory Mechanism of Electro-acupuncture in Irritable Bowel Syndrome:Preventing MC Activation and Decreasing SP VIP Secretion[J]. Dig Dis Sci,2007,13 [Epub ahead of print].
[50]李兆申,董文珠,邹多武,等.肠易激综合征肠黏膜SP、VIP、CGRP变化的研究[J].第二军医大学学报,2003,24(2)：147-150.
[51]梁荣新,蔡联英,郑琴芳.肠易激综合征患者乙状结肠动力及胃肠激素变化的研究,广西医科大学学报,2003,20(6)：843-845.
[52]林琳,赵志泉,殷咏梅,等.胃肠激素与肠易激综合征的相关性[J].江苏医药,2001,27(3)：166-8.
[53]陈敏,TNF-α及PGE2与小儿全身炎症反应综合征[J].内蒙古医学杂志Inner Mongolia Med J 2012,44(10),1209-1210.
[54]应延风,陈浩浩,金耀建,等.PGE2对气道平滑肌细胞分泌和凋亡影响的研究[J/CD].中华哮喘杂志,2012,6(4)：239-244.
[55]林琳,赵志泉,殷咏梅,等.胃肠激素与肠易激综合征的相关性[J].江苏医药杂志,2001,27(3)：166-168.
[56]Husum H,Van Kammen D,Termeer E, et al. Neuropsychopharmacology,2003,9:422-428.
[57]Chiocchetti R,PooleDP,KimuraH,et al. Cell Tissue Res,2003,311:11-22
[1]张声生,李乾构,魏玮,等.肠易激综合征中医诊疗共识意见[J].中华中医药杂志,2010,25：1062-1065.
[2]Longstreth GF, Thompson WG, Chey WD, et al. Functional bowel disorders. Gastroenterology,2006,130:1480-1419.
[3]Spinelli A. Irritable bowel syndrome. Clin Drug Investig,2007; 27:15-33.
[4]Xiong LS, Chen MH, Chen HX, Xu AG, Wang WA,Hu PJ. A population-based epidemiologic study of irritable bowel syndrome in South China:stratifi ed randomized study by cluster sampling. Aliment Pharmacol Ther 2004; 19:1217-1224.
[5]张万岱.功能性胃肠病罗马标准的简介和解读[J.]世界华人消化杂志.2008,16：120-124.
[6]张文静.成人肠易激综合征诊治新指南[J].现代消化及介入诊疗.2007,12：141.
[7]孙菁,袁耀宗.肠易激综合征：罗马III标准与我国共识意见解读[J].医学与哲学·临床决策论坛版.2008,29：12-16.
[8]Williams CL,Villar RG,Peterson JM,Burks TF.Stress-induced changes in intestinal transit in the rat:a model for irritable bowel syndrome.Gastroenterology,1988,94:611-621.
[9]吕红,王伟岸,钱家鸣.传统束缚应激动物模型内脏感觉的新评价[J].胃肠病学和肝病学杂志,2005,14：111-113.
[10]吴晓亮,孙建华.肠易激综合征国内实验研究现状的调查[J].世界华人消化杂志.2010,18(35)：3818-3823.
[11]任天华,胡品津,胡志远,等.新生期母婴分离对大鼠成年后结直肠扩张后5-羟色胺表达的影响[J].中山大学学报,2004,25(3)：234.
[12]刘雁冰,袁耀宗,陶然君,等.大鼠肠道高敏性模型的建立及其内脏敏感性评估[J].中华消化杂志,2003,23(1)：34-37.
[13]Al Chaer ED, Kawasaki M, Pasricha P J. A new of chronicvis ceralhy persensitivity in adult rats induced by colon irritation during postnatal development [J]. Gastroenterology, 2000,119(5):1276.
[14]李延青,杨军.肠易激综合征内脏感觉过敏动物模型的建立[J].胃肠病学和肝病学杂志,2003,12(4)：332.
[15]彭丽华,孙刚,杨云生.便秘型肠易激综合征新概念模型的建立.世界华人消化杂志,2004,12：112-116.
[16]La JH, Kim TW, Sung TS, et al. Visceral hypersensitivity and altered colonic motility after subsidence of inflammation in a rat model of colitis [J]. World J Gastroenterol, 2003,9:2791-2795.
[17]O'Mahony SM,Marchesi JR,Scully P,Codling C,Ceolho AM,Quigley EM,Cryan JF,Dinan TG. Early life stress alters behavior,immunity,and microbiota in rats:implications for irritable bowel syndrome and psychiatric illnesses.Biol Psychiatry2009;65:263-267.
[18]WILLIAMS C L, VILLAR R G, PETERSON J M, et al. Stress- induced changes in intestinal transit in the rat:a model for irritable bowel syndrome[J]. Gastroenterology,1988, 94:611.
[19]LI Z D, LI D G, WU J C,et al.Effects and mechanism of CRF microinjection into PVN on gastric emptying[J]. J SSMU,2000,12:103.
[20]刘清华,李定国.肠易激综合征动物模型复制思路和方法[J].国外医学：消化系疾病分册,2002,40：154-156.
[21]Collions SM, McHugh K, Jacobson K, et al. Prerious inflammation alters the response of the rat colon to stress. Gastroenterology,1996,111:1509-1515.
[22]郭敏,李延青,于秀娟,孔庆莲等.大鼠急性肠道感染后肠功能紊乱动物模型的建立.山东大学学报(医学版),2006,44(6)：586-589.
[23]王伟岸,钱家鸣,潘国宗.肠易激综合征的发病机制[J].胃肠病学和肝病学杂志,2001,10：103-105.
[24]刘清华.肠易激综合征动物模型复制思路及方法[J].国外医学(消化系疾病分册),2002,22：154-156.
[25]彭丽华,杨云生,孙刚,等.便秘型肠易激综合征新概念模型的建立[J].世界华人消化杂志,2004,12：112-116.
[26]Coutinho S, Plotsky PM, SabladM,etal. Neonatalmater-nal separation alters stress-induced response to visceroso-matic nociceptive stimuli in rats[J]. Am J PhysiolGstroi-ntestLiverPhysio,2002,282:307-316.
[27]O'Mahony SM, Marchesi JR, Scully P, Codling C, Ceolho AM, Quigley EM, Cryan JF, Dinan TG. Early life stress alters behavior, immunity, and microbiota in rats:implications for irritable bowel syndrome and psychiatric illnesses. Biol Psychiatry 2009; 65:263-267.
[28]朱力阳,彭成,谢晓芳,王嫣虹.化学刺激引起的两种大鼠内脏高敏感性模型的建立[J].中国药理学通报2010；26：267-270.
[29]徐俊荣,罗金燕.肠易激综合征动物模型研究进展[J].基础医学与临床,2006,26(8)：902-904.
[30]Collins SM, Mchugh K, Jacobson K,et al. Previous in-flammation alters the response of the rat colon to stress[J]. Gastroenterology,1996,111:1509-1515.
[31]Smith JL,Bayles D.Postinfectious irritable bowel syndrome:a longterm consequence of bacterial gastroenteritis.J Food Prot,2007,70:1762-1769.
[32]De Giorgio R,Barbara G.Is irritable bowel syndrome an inflammatory disorder?Curr Gastroenterol Rep,2008,10:385-390.
[33]Barbara G, Vallance BA, Collins SM. Persistent intestinal neuron muscular dysfunction after acute nematode infection in mice. Gastroenterology,1997,113:12242-12321.
[34]Toulouse M, Coelho AM, Fioramonti J, et al. Role of tachykinin NK2 receptors in normal and altered rectal sensitity in rats. Br J Pharmacol,2000,129:193-199.
[35]朱力阳,彭成,谢晓芳,等.化学刺激引起的两种大鼠内脏高敏感性模型的建立[J].中国药理学通报.2010,26(2)：267-270.
[36]Al Chaer ED, Kawasaki M, Pasricha P J. A new of chronicvis ceralhy persensitivity in adult rats induced by colon irritation during postnatal development [J]. Gastroenterology, 2000,119(5):1276.
[37]刘雁冰,袁耀宗,陶然君,等.大鼠肠道高敏性模型的建立及其内脏敏感性评估[J].中华消化杂志,2003,23(1)：34-37.
[38]Lomax AE, Fernandez E, Sharkey KA. Plasticity of the enteric nervous system during intestinal inflammation[J]. Neurogastroenterol Motil,2005,17(1):4-15.
[39]Tornblom H, Lindberg G, Nyberg B, Veress B. Full-thickness biopsy of the jejunum reveals inflammation and enteric neuropathy in irritable bowel syndrome. Gastroenterology 2002; 123:1972-1979.
[40]Vasina V, Barbara G, Talamont L, et al.Enteric neuroplasticity evoked by inflammation[J].Auton Neurosci,2006,30(126-127):264-272.
[41]朱雪萍,陈卫昌,朱晓黎,等.脑肠肽、脑肠轴在肠易激综合征模型中作用机制研究[J].临床儿科杂志2008,26：236-239.
[42]王伟岸,钱家鸣,潘国宗,等.脑-肠互动指向性条件应激肠易激综合征动物模型的建立[J].中华消化杂志,2004；24：590-593
[43]Lu H, Qian JM, Jin GL, Zhou DF, Shen YC. [Theestablishment of an animal model of gut-brain interaction in irritable bowel syndrome for the evaluation of visceral sensation, motility and psychological behavior.] Zhonghua Neike Zazhi 2009; 48:1035-1039
[44]吴晓亮,孙建华.肠易激综合征国内实验研究现状的调查[J],世界华人消化杂志,2010,18(35)：3818-3823.
[45]Al-chaer ED,Kawasaki M,Pasricha PJ.A new model of chronic visceral hypersensitivity in adult rats induced by colon irritation during postnatal development [J].Gastroenterology,2000,119:1276-1285.
[46]Mayer EA,Gebhart GF.Basic and clinical aspects of visceral hyperalgesia[J].Gastroenterology,1994,107:271-293.
[47]Soderholm JD, Yates DA, Gareau MG, et al. Neonatal maternal separation predisposes adult rats to colonic barrier dysfunction in response to mild stress[J]. Am J Physiol Gastrointest Liver Physiol,2002,283:1257-1263.
[48]蔡淦,张正利.电刺激-束缚大鼠肠易激综合征模型的应用与评价[J].中国中西医结合消化杂志,2005,13(2)：75-77.
[49]刘新光.模拟肠易激综合征动物动物模型研究的评述[J].胃肠病学和肝病学杂志,2004,12：329-330.
[50]桂先勇.寒冷-束缚应激对大鼠结肠运动的后续效应[J].中华消化杂志,1997,17(2)：94-96.
[51]Eutamene H,Bradesi S,Larauche M,Theodorou V,Beaufrand C,Ohning G,Fioramonti J,Cohen M, Bryant AP.Kurtz C,Currie MG,Mayer EA,Bueno L.Guanylate cyclase C-mediated antinociceptive effects of linaclotide in rodent models of visceral pain. Neurogastroenterol Motil 2010;22:312-384.
[52]李冬华,李春淼,李伍善,邹志东,张炎.痛泻要方对肠易激综合征模型大鼠血管活性肠肽的影响.时珍国医国药2007；18：2098-2099
[53]费晓燕,谢建群,郑昱,吴大正,袁建业.疏肝饮对腹泻型肠易激综合征模型大鼠胃动素和胆囊收缩素的影响.上海中医药杂志2008；42：63-65
[54]李丹,吕妍,唐方.腹泻型肠易激综合征大鼠模型的建立[J].天津中医药.2009,26(3)：240-242.
[55]白殿卿,孙晓宁,安钰,郑颖.大鼠肠易激综合征模型的建立及其评价.中国实验动物学报,2009,17(5)：377-379.
[56]安钰,白殿卿,付健,等.乙酸灌肠加束缚应激致大鼠肠易激综合征模型的建立及其评价[J].世界华人消化杂志2009；17：1548-1551.
[57]王伟岸,钱家鸣,潘国宗,等.脑-肠互动指向性条件应激肠易激综合征动物模型的建立[J].中华消化杂志,2004,24(10)：590-593.
[58]徐海珍,谢建群,施斌,等.腹泻型肠易激综合征大鼠结肠黏膜炎性细胞因子的表达及温中健脾方对其影响的研究[J].上海中医药杂志,2007,41(6)：69.
[59]石君杰,王海云,戴玉英,等.逍遥散对肠易激综合征大鼠腹壁肌电活动紊乱调节作用的机制探讨[J].浙江中医杂志,2008,43(3)：139.
[60]赵文娟,王垂杰.腹泻型肠易激综合征大鼠模型的建立[J].国际中医中药杂志,2008,30(1)：16.
[61]胡萍萍,吕宾,范一宏.内脏高敏感大鼠脑部5-羟色胺表达差异的研究[J].中华消化杂志,2006,26(5)：347.
[62]Wingate D,Hongo M, Kellow J,et al.Disorders of gastrointestinal motility:towards a new classification.J Gastroenterol Hepatol.2002,17 (Suppl):S1-14.
[63]Kalantar JS, Locke GR, Zinsmeister AR, et al. Familial agg-regation of irritable bowel syndrome:a prospective study[J]. Gut,2003,52:1703-1707.
[64]罗小雨,钟良.肠易激综合征发病机制的研究进展[J],国际消化病杂志,2010,30(6)：321-324.
[65]Sinhamahapatra P, Saha SP, Chowdhury A,et al. Visceral afferent hypersensitivity in irritable bowel syndrome evaluation by cerebral evoked potential after rectal stimulation[J]. Am J Gastroenterol,2001,96(7):2150-2157.
[66]Nozu T,KudairaM.Altered rectal sensory response induced by balloon distention in patients with functional abdominal pain syndrome[J]. Biopsychosoc Med,2009,3:13.
[67]张鸣鸣,姜敏.肠易激综合征发病机制研究进展[J].世界华人消化杂志,2009,17：2484-2490.
[68]ChadwickVS, ChenW, Shu D,etal. Activation of the mucosal im-mune system in irritable bowel syndrome[J]. Gastroenterology,2002,122(7):1778-1783.
[69]PimentelM, Chow EJ, Lin HC. Normalization of lactulose breathtesting correlateswith symptom improvement in irritable bowe syn-drome:a double-blind, randomized, placebo-controlled study[J].Am JGastroentero,1 2003,98(2):412-419.
[70]谢启文.神经肽[M].上海：复旦大学出版社,2004,167-169.
[71]李继锋,王舟,纪祥瑞.降钙素基因相关肽在神经免疫内分泌网络中的作用[J].中国神经免疫学和神经病学杂志,2004,1(3)：177-179.
[72]Keith IM. The role of endogenous lung neuropeptides in regulation of the pulmonary circulation[J].Physiol Res,2000;49(5):519-537.
[73]Van Geldre LA,Lefebvre RA. Interaction of NO and VIPin gastrointestinal smooth muscle relaxation[J].CurrPharm Des,2004,10(20):2483-2497.
[74]Evangelista S.Involvement of tachykinins in intestinal inflammation[J].Curr Pharm Des,2001,7(1):19-30.
[75]陈敏TNF-a及PGE2与小儿全身炎症反应综合征[J].内蒙古医学杂志Inner Mongolia M ed J 2012,44(10):1209-1210.
[76]陶琳,柯美云,王智凤.小肠细菌过度生长与功能性胃肠疾病的关系[J].中国中西医结合消化杂志,2005,13(2)：122-123.
[77]Portincasa P, Moschetta A, Baldassarre G, Altomare DF, Palasciano G. Pan-enteric dysmotility,impaired quality of life and alexithymia in a large group of patients meeting ROME II criteria for irritable bowel syndrome[J]. World J Gastroenterol,2003;9:2293-2299.
[78]Small PK, Loudon MA, Hau CM, Noor N, Campbell FC. Large-scale ambulatory study of postprandial jejunal motility in irritable bowel syndrome[J]. Scand J Gastroenterol, 1997;32:39-47.
[79]AL-Chaer E D, Kawasaki M, Pasricha P J. A new model of chronic visceral hypersensitivity in adult rats induced by colon irritation during postnatal development [J]. Gastroenterology,2000,119:1276-1285.
[80]刘雁冰,袁耀宗,陶然君,等.大鼠肠道高敏感性模型的建立及其内脏敏感性评估[J].中华消化杂志,2003,23：34-37.
[81]李延青,杨军.肠易激综合征内脏感觉过敏动物模型的建立[J].胃肠病学和肝病学杂志,2003,12(4)：332.
[82]白殿卿,孙晓宁,安钰,等.大鼠肠易激综合征模型的建立及其评价[J].中国实验动物学报,2009,17(5)：377-379.
[83]郭玉婷,李延青,杨军,等.肠易激综合征内脏过敏大鼠脊髓诱发电位的研究[J].胃肠病学,2004,9(6)：347.
[84]李兆申.肠易激综合征内脏高敏感性研究现状和展望.第二军医大学学报.2003；24(2)：120-123.
[1]张声生,李乾构,魏玮,等.肠易激综合征中医诊疗共识意见[J].中华中医药杂志,2010,25：1062-1065.
[2]Longstreth GF, Thompson WG, Chey WD, et al. Functional bowel disorders. Gastroenterology,2006,130:1480-1419.
[3]赵雅芳,李春华,稽波,等.电针三阴交、血海对痛经模型大鼠子宫微循环的影响[J].微循环杂志,2011,21(2)：4-7.
[3]ZhaoYa-fang, Li Chun-hua, Ji Bo, et al. Effect of electroacupuncture at Sanyinjiao and Xuehai on uterine miocrocirculation in dysmenorrhea Rats[J]. Chinese Journal of Microcirculation,2011,21(2):4-7.
[4]任晓暄,郭孟玮,赵雅芳,等.电针对大鼠类痛经反应、脊髓K-受体表达及中脑导水管周围灰质脑啡肽和p-内啡肽含量的影响[J].针刺研究,2012,37(1)：1-7.
[4]Ren Xiao-xuan,Guo Meng-wei, Zhao Ya-fang, et al. Effect of electroacupuncture on pain reactions, expression of spinal κ-opioid receptor and contents of enkephalin and (3-endorphin in periaqueductal gray of midbrain in dysmenorrheal model rats[J]. Acupuncture Research, 2012,37(1):1-7
[5]AL-Chaer E D,Kawasaki M,Pasricha P J.A new model of chronic visceral hypersensitivity in adult rats induced by colon irritation during postnatal development [J].Gastroenterology, 2000,119:1276-1285.
[6]刘雁冰,袁耀宗,等.大鼠肠道高敏感性模型的建立及其内脏敏感性评估[J].中国消化杂志,2003,23：34-37.
[7]Al-chaer ED, Kawasaki M, Pasricha PJ. A new model of chronic visceral hypersensitivity in adult rats induced by colon irritation dur-ing postnatal development. Gastroenterology, 2000,119(115):276-1285.
[8]Mayer EA,Collins SM. Evolving PathoPhysiologic models of functional gastrointestinal disorders [J]. Gastroenterology,2002,122:2032-2048.
[9]王伟岸,钱家鸣,潘国宗,等.脑-肠互动指向性条件应激肠易激综合征动物模型的建立[J].中华消化杂志,2004,24：590-593.
[10]曾昭炜,谢忠明,唐荣福等.观察动物微循环的实验方法[J]微循环学杂志,2011,21(3)：26-32.
[11]田牛.微循环方法学[M].北京：原子能出版社,1993：175.
[12]AL-Chaer E D, Kawasaki M, Pasricha P J. A new model of chronic visceral hypersensitivity in adult rats induced by colon irritation during postnatal development [J]. Gastroenterology,2000,119:1276-1285.
[13]Bouin M, Plourde V, Boivin M, et al. Rectal distention testing in patients with irritable bowel syndrome:sensitivity, specificity, andpredictive values of pain sensory thresholds. Gastroenterology,2002,122(7):1771-1777.
[14]袁耀宗,刘雁冰,陶然君等.肥大细胞在应激所致大鼠直肠高敏感性中的作用[J].中华消化杂志,2003,23(12)：727-730.
[15]赵宁侠,郭瑞林,任秦有.针灸治疗原发性痛经临床疗效及血液流变学相关性分析[J].浙江中医药大学学报,2007,31(3)：364-367.
[16]周慧敏,张庆富,张晓琳.肠系膜微循环研究的若干进展.中国微循环,2006,10(3)：217-219.
[17]赵雅芳,李春华,嵇波,等.电针三阴交、血海对痛经模型大鼠子宫微循环的影响[J].微循环学杂志,2011,21(2)：4-7.
[2]Longstreth GF, Thompson WG, Chey WD, et al. Functional bowel disorders. Gastroenterology,2006,130:1480-1419
[3]Harris LA,Hansel S,DiBaise J,et al.Irritable bowel syndrome and chronic constipation:emerging drugs,devices,and surgical treatments.Curr Gastroenterol Rep,2006,8:282-290.
[4]Spinelli A. Irritable bowel syndrome. Clin Drug In-vestig 2007,27:15-333 Xiong LS, Chen MH, Chen HX, Xu AG, Wang WA,Hu PJ. A population-based epidemiologic study of irritable bowel syndrome in South China:
[5]潘国宗,鲁素彩,柯美云,等.北京地区肠易激综合征的流行病学研究：一个整群、分层、随机的调查.中华流行病学杂志,2000,21：26-29.
[6]熊理守,陈旻湖,陈惠新,等.广东省社区人群肠易激综合征的流行病学研究.中华医学杂志,2004,84：278-281.
[7]Wingate D,Hongo M, Kellow J, et al.Disorders of gastrointestinal motility:towards a new classification.J Gastroenterol Hepatol,2002,17(Suppl):S 1-14.
[8]Andresen V, Camilleri M. Irritable bowel syndrome:recent and novel therapeutic approaches. Drugs 2006,66:1073-1088
[9]O'Mahony SM, Hyland NP, Dinan TG, Cryan JF. Maternal separation as a model of brain-gut axis dysfunction. Psychopharmacology (Berl)2010 Oct 1 [Epub ahead of print] PMID:20886335
[10]Sheehan J, Gaman A, Vangel M, Kuo B. Pooled analysis of brain activity in irritable bowel syndrome and controls during rectal balloon distension. Neurogastroenterol Motil 2010 Nov 30. doi:10.1111/j.1365-2982.2010.01635.x.
[11]Elsenbruch S. Abdominal pain in irritable bowel syndrome:a review of putative psychological, neural and neuro-immune mechanisms. Brain Behav Immun 2010 Nov 20.
[12]Ohman L,Simren M. Pathogenesis of IBS:role of inflammation, immunity and neuroimmune interactions. Nat Rev Gastroenterol Hepatol 2010,7:163-73. Epub 2010 Jan 26.
[13]Park MI,Camilleri M.Is there a role of food allergy in irritable bowel syndrome and functional dyspepsia? A systematic review.Neurogastroenterol Motil,2006,18:595-607.
[14]Chang L,Mayer EA,Labus JS,et al. Effect of sex on perception of rectosigmoid stimuli in irritable bowel syndrome.Am J Physiol Regul Integr Comp Physiol,2006,291:R277-284.
[15]Houghton LA,Jackson NA,Whorwell PJ,et al.Do male sex hormones protect from irritable bowel syndrome? Am J Gastroenterol,2000,95:2296-2300.
[16]Heitkemper MM,Jarrett ME.Update on irritable bowel syndrome and gender differences.Nutr Clin Pract,2008,23:275-283.
[17]Ruigomez A,Garcia Rodriguez LA,Johansson S,et al.Is hormone replacement therapy associated with an increased risk of irritable bowel syndrome? Maturitas,2003,44:133-140.
[18]Naliboff BD,Berman S,Chang L,et al.Sex-related differences in IBS patients:central processing of visceral stimuli.Gastroenterology,2003,124:1738-1747.
[19]Smith JL,Bayles D.Postinfectious irritable bowel syndrome:a longterm consequence of bacterial gastroenteritis.J Food Prot,2007,70:1762-1769.
[20]De Giorgio R,Barbara G.Is irritable bowel syndrome an inflammatory disorder? Curr Gastroenterol Rep,2008,10:385-390.
[21]Spiler R,Campbell E.Post-infectious irritable bowel syndrome[J].Curropin Gastroenterol, 2007,9(5):378-384.
[22]O'Mahony L,McCarthy J,Kelly P,et al.Lactobacillus and bifidobacterium in irritable bowel syndrome:symptom responses and relationship to cytokine profiles.Gastroenterology, 2005,128:541-551.
[23]Lin H.Small intestinal bacterial overgrowth:a framework for understanding irritable bowel syndrome.JAMA,2004,292:852-858.
[24]张茹.胃肠激素与肠易激综合征[J].标记免疫分析与临床,2004,11(1)：44-46.
[25]刘丽宏,梅其炳,陈蕾等.5-羟色胺对培养胃底平滑肌细胞内游离钙动员的影响[J].第四军医大学学报,2001,22(10)：891.
[26]Chey WY,Jin HO,Lee MH,et al.Colonic motility abnormality in patients with irritable bowel syndrome exhibiting abdominal pain and diarrhea.Am J Gastroenterol,2001,96:1499-1506.
[27]Sagami Y,Hongo M.The gastrointestinal motor function in irritable bowel syndrome(IBS).Nippon Rinsho,2006,64:1441-1445.
[28]Sagami Y,Shimada Y,Tayama J,et al.Effect of a corticotropin releasing hormone receptor antagonist on colonic sensory and motor function in patients with irritable bowel syndrome.Gut,2004,53:958-964.
[29]Kalantar JS, Locke GR, Zinsmeister AR, et al. Familial agg-regation of irritable bowel syndrome:a prospective study[J]. Gut,2003,52:1703-1707.
[30]Kanazawa M,Endo Y,Whitehead WE,et al.Patients and nonconsulters with irritable bowel syndrome reporting a parental history of bowel problems have more impaired psychological distress.Dig Dis Sci,2004,49:1046-1053.
[31]Kim HJ, Camilleri M, Carlson PJ, et al. Association of distinct alpha(2) adrenoceptor and serotonin transporter polymorphisms with constipation and somatic symptoms in functional gastrointestinal disorders[J]. Gut,2004,53:829-837.
[32]Gonsalkorale WM, Perrey C, Pravica V, et al. InterleukinlO genotypes in irritable bowel syndrome:evidence for an inflammatory component[J],Gut,2003,52:91-93.
[33]罗小雨,钟良.肠易激综合征发病机制的研究进展[J],国际消化病杂志,2010,30(6)：321-324.
[34]Hayee B,Forgacs I.Psychological approach to managing irritable bowel syndrome.BMJ,2007,334:1105-1109.
[35]Endo Y.Psychological symptoms in IBS.Nippon Rinsho,2006,64:1471-1476.
[36]Hagymasi K,Tulassay Z.Genetic background of irritable bowel syndrome.Orv Hetil, 2006,147:1167-1170.
[37]Bouin M,Plourde V,Boivin M,et al.Rectal distention testing in patients with irritable bowel syndrome:sensitivity,specificity,andpredictive values of pain sensory thresholds.Gastroenterology,2002,122:1771-1777.
[38]Mayer EA.The neurobiology of stress and gastrointestinal disease.Gut,2000,47:861-869.
[39]Poitras P,Riberdy Poitras M,Plourde V,et al. Evolution of visceral sensitivity in patients with irritable bowel syndrome[J].Dig Dis Sci,2002,47(4):914-920.
[40]Schwartz MP, Samsom M, Smout AJ. Chemospecific alterations in duodenal perception and motor response in functional dyspepsia. Am J Gastroenterol 2001,96(9):2596-2602
[41]Kaneko H,Imamura H,Konagaya T.Visceral hypersensitivity.Nippon Rinsho,2006,64:1446-1451.
[42]Posserud I,Agerforz P,Ekman R,et al.Altered visceral perceptual and neuroendocrine response in patients with irritable bowel syndrome during mental stress.Gut,2004,53:1102-1108.
[43]Dickhaus B,Mayer EA,Firooz N,et al. Effect of auditory stress on perceptual and emotional ratings of visceral stimuli in IBS patients. Am J Gastroenterol,2003,98:135-143.
[44]胡品津.从脑-肠互动的高度认识肠易激综合征[J].中华消化杂志,2003,23(5)：261.
[45]Talley NJ,Howell S,Poulton R.The irritable bowel syndrome and psychiatric disorders in thecommunty:is there a link[J].Am J gastroenterol,2001,96(4):1072.
[46]Mayer EA,Berman S,Suyenobu B,et al.Differences in brain responses to visceral pain between patients with irritable bowel syndrome and ulcerative colitis.Pain,2005,115:398-409.
[47]Yuan YZ,Tao RJ,et al.Functional brain imaging in irritable bowel syndrome with rectal balloon-distention by using MRI.World J Gastroenterol,2003,9(6):1356.
[48]Chang L,Berman S,Mayer EA,et al.Brain responses to visceral and somatic stimuli in patients with irritable bowel syndrome with and without fibromyalgia.Am J Gastroenterol,2003,98:1354-1361.
[49]Wilder-Smith CH,Schindler D,Lovblad K,et al.Brain functional magnetic resonance imaging of rectal pain and activation of endogenous inhibitory mechanisms in irritable bowel syndrome patient subgroups and healthy controls.Gut,2004,53:1595-1601.
[50]Longstreth GF,ThbmPson WG,Chey WD,et al. Functional bowel disorders.Gastroe-nterology,2006,130:1480-1491.
[51]O'Donnell LJ,Virjee J,Heaton KW.Detection of pseudodiarrhoea by simple clinical assessment of intestinal transit rate.BMJ,1990,300:439-440.
[52]张琳,周孝德,赵国福,等.肠易激综合征中西医研究进展[J].甘肃中医.2003,16(2)：46-48.
[53]王永炎.中医内科学.上海科学技术出版社.1997：172-201.
[54]周福生,吴文江,黄志新.顺激合剂治疗肠易激综合征疗效观察[J].广州中医药大学学报.2002,19(4)：269-271.
[55]胡旭光,张思为.疏肝、健脾、疏肝健脾方药对肠易激综合征模型大鼠的治疗作用及机理探讨[J].河南中医,2007,27(9)：23-24.
[56]杨银芳,楚更五,张建.痛泻要方对寒冷-束缚肠易激综合征模型大鼠作用的实验研究[J].中华中医药学刊,2008,26(9)：1984-1986.
[57]朱力阳,彭成,彭延娟.四逆散治疗大鼠肝脾不调证肠易激综合征模型的研究.四川中医[J],2010,28(8)：10-11.
[58]李浩,孙建华.针灸治疗肠易激综合征临床研究概况及机制探讨.中国中医急症[J],2011,20(3)：430-431.
[59]骆燕宁,孙彩霞.针灸治疗结肠易激症群158例[J].上海针灸.2003,22(4)：28.
[60]段彩琴,李爱萍.温针治疗肠易激综合征疗效观察[J].中国针灸.2001,21(4)：226.
[61]王美娟.针刺夹脊穴治疗肠易激综合征32例[J].湖南中医药导报.2002,8(5)：272-273.
[62]安广青,李娜,翟国华,等.针灸治疗肠易激综合征疗效评价[J].上海针灸杂志.2010,29(6)：354-356.
[63]何严,高宏.针灸配合按摩治疗肠易激综合征36例[J].陕西中医.2004,25(3)：258.
[64]王伟.温针治疗肠易激综合征腹泻型临床观察[J].中国中医急症.2008,17(10)：1377-1407.
[65]吴向琼.温针并耳压治疗肠易激综合征46例临床观察[J].湖南中医药导报.2004,10(3)：42.
[66]高军,韩景献.针刺治疗肠易激综合征32例.针灸临床杂志[J],2010,26(9)：24-25.
[67]王文文.穴位埋线法治疗肠易激综合征33例体会[J].现代中西医结合杂志.2002,11(4)：328-329.
[68]丁淑强,李大军.承山穴为主治疗肠易激综合征54例疗效观察[J].针灸临床杂志,2004,20(5)：53.
[69]刘立功,顾杰.急病针灸典籍通览[M].上海：上海科学技术出版社,2000.
[70]黄琴峰.针灸治疗便秘临床规律探讨.辽宁中医杂志,2009,36(3)：368-369.
[71]高巍,黄裕新,张洪新,等.电针“足三里”对大鼠脑肠肽含量的影响及其对免疫系统的调控作用.针刺研究[J].2002,27(1)：50-54.
[72]常小荣,严洁,刘玉群,等.电针足三里和阳陵泉穴对家兔胃胆运动及脑肠肤的影响.世界华人消化杂志.2006,14(17)：1662-1668
[73]吴亚丽,陈少夫,潘莉莉.电针对胃炎患者胃酸分泌及胃肠激素的影响[J].中国中西医结合杂志,1994,14(12)：709.
[74]Sun DY,Huang YX,Cu ZH,Gao W,Wang QL.Effects of electroacupuncture on the pressure in pylonic antrum and brain-gut peptide content in dogs.Shijie Huaren Xiaohua Zazhi,1999;7:997-999.
[75]Huang YX, Sun DY, Chu ZH,Gao W, Wang QL. Effect of electroacupunture on canine pyloric pressure and its relation to somatostatin and nitric oxide. Shijie Zhenjiu Zazhi, 1999,9:43-46.
[1]Spiegel BM, Gralnek IM, Bolus R, Chang L, Dulai GS, Mayer EA, Naliboff B. Clinical determinants of health-related quality of life in patients with irritable bowel syndrome[J]. Arch Intern Med 2004,164:1773-1780.
[2]胡品津.从脑-肠互动的高度认识肠易激综合征[J].中华消化杂志,2003,23(5)：261-262.
[3]Talley NJ,Howell S,Poulton R.The irritable bowel syndrome and psychiatric disorders in thecommunty:is there a link? [J] Am J gastroenterol,2001,96(4):1072.
[4]Hayee B,Forgacs I.Psychological approach to managing irritable bowel syndrome.BMJ,2007,334:1105-1109.
[5]吴晓亮,孙建华.肠易激综合征国内实验研究现状的调查[J].世界华人消化杂志,2010,18(35)：3818-3823.
[6]Gaman A, Kuo B. A review of CNS activation patterns from fMRI studies in IBS patients versus healthy controls. Gastroenterology 2007;32(4 supplement 2):A-602.
[7]Berman S,Munakata J,Naliboff BD,et al.Gender differencesin regional brain response to visceral pressure in IBS patients [J].Eur J Pain,2000,4(2):157-172.
[8]Bonaz B,Baciu M,Papillon E,et al.Central processing of rectal pain in patients with irritable bowel syndrome:an fMRI study[J].Am J Gastroenterol,2002,97(3):654-661.
[9]Accarino AM,Azpiroz F,Malagelada JR.Attention and distraction:effects on gut perception[J]. Gastroenterology,1997,113(2):415-422.
[10]Smith JL,Bayles D.Postinfectious irritable bowel syndrome:a longterm consequence of bacterial gastroenteritis.J Food Prot,2007,70:1762-1769.
[11]De Giorgio R,Barbara G.Is irritable bowel syndrome an inflammatory disorder?Curr Gastroenterol Rep,2008,10:385-390.
[12]朱力阳,彭成,谢晓芳,等.化学刺激引起的两种大鼠内脏高敏感性模型的建立[J].中国药理学通报,2010；26：267-270.
[13]RINGEL Y,DROSSMAN D A, TURKINGTON T G, et al. Dysfunctions of the mofivational affective pain System in patients with IBS:PET brain imaging in response to rectal ballon distention[J]. Gastronterology,2000, (Mav Suppl):A474.
[14]Mayer EA,Berman S,Suyenobu B,et al.Differences in brain responses to visceral pain between patients with irritable bowel syndrome and ulcerative colitis.Pain,2005, 115:398-409.
[15]Ringel Y,Prossman DA,Tukington TG,et al.Dysfunction of the motivational-affective pain system in patients with IBS:PET brain imaging in response to rectal bulloon distension.Gastroenterology,2000,9 (4Suppl):410
[16]Wilder-Smith CH,Schindler D,Lovblad K,et al.Brain functional magnetic resonance imaging of rectal pain and activation of endogenous inhibitory mechanisms in irritable bowel syndrome patient subgroups and healthy controls. Gut,2004,53:1595-1601.
[17]李兆申.肠易激综合征内脏高敏感性研究现状和展望.第二军医大学学报.2003,24(2)：120-123
[18]Gao J,Wu X,Owyang C,et al.Enhanced responses of the anterior Cingulated cortex neurones to colonic distension in viscerally hypersensitive rats[J].J Physiol,2006,570 (Pt-1):169-183.
[19]袁耀宗,陶然君,许斌,等.肠易激综合征患者直肠气囊扩张时的功能脑显像研究[J].Chinese Journal of Gastroenterol.2003,8(supplement):4
[20]Casey KL,Minoshima S,Berger KL,et al.Positron emission tomographic analysis of cerebral structures activated specifically by repetitive noxious heat stimuli[J].J Neurosurg, 1994,71(2):802-807.
[21]Derbyshire SW,Jones AK,Devani P,et al.Cerebral responses to pain in patients with atypical facial pain measured by positron emission tomography[J].J Neurol Neu-rosurg Psychiatry,1994,57(10):1166-1172
[22]Bouin M, et al. Rectal distention testing in patients with irritable bowel syndrome: sensitivity, specificity, and predictive values of pain sensory thresholds. Gastroenterology 2002,122(7):1771-1777
[23]Mertz H, et al. Altered rectal perception is a biological marker of patients with irritable bowel syndrome. Gastroenterology,1995,109(1):40-52
[24]Cervero F, Janig W. Visceral nociceptors:a new world order? Trends Neurosci.1992, 15(10):374-378
[25]De Ponti F·Pharmacology of serotonin:what a clinician should know[J].Gut,2004, 53(10):1520
[26]Pan H,Gershon MD · Activation of intrinsic afferent pathways in submucosal ganglia of the guinea pig small intestine[J].J Neurosci,2000,20:3295
[27]Poitras P,Riberdy Poitras M,Plourde V,et al. Evolution of visceral sensitivity in patients with irritable bowel syndrome[J].Dig Dis Sci,2002,47(4):914-920.
[28]Sinhamahapatra P,Saha SP,Chowdhury A, Chakrabarti SK,Ghosh A,Maiti B.Visceral afferent hypersensitivity in irritable bowel syndrome-evaluation by cerebral evoked potential after rectal stimulation.Am J Gastroenterol 2001; 96:2150-2157
[29]Malcolm A,Phillips SF,Kellow JE,Cousins MJ.Direct clinical evidence for spinal hyperalgesia in a patient with irritable bowel syndrome.Am J Gastroenterol 2001; 96:2427-2431
[30]Fukudo S, Nomura T, Muranaka M,et al. Brain-gut response to stress and cholinergic stimulation in irritable bowel syndrome. A preliminary study [J]. J Clin Gastroentero,l 1993, 17(2):133-141.
[31]董文珠,邹多武,李兆申,许国铭等.肠易激综合征患者内脏高敏性的机制研究.中华消化杂志2004；24：18-22
[32]李延青.什么导致了肠易激综合征.临床消化病杂志.2009,21(2)：110-111
[33]Elsenbruch S, OrrWC. Diarrhea and constipation-predominant IBS patients differ in postprandial autonomic and cortisol responses[J].Am J Gastroentero,l 2001,96(2):460.
[34]孙燕,刘锋霖,宋耿青,等.急性和慢性束缚应激对大鼠内脏敏感性和神经内分泌的影响[J].中华消化杂志,2006,26(1)：38
[35]Talley NJ,Fett SL,Zinsinerster AR,et al·Gastrointestinal tract symptoms and self-reported abuse:a population-based study[J]. Gastroenterology,1994,107(9):1040
[36]Goyal RK, Hirano I. The enteric nervous system[J]. N Engl J Med,1996,334(17): 1106-1115
[37]Basilisco G. Pathogenesis of irritable bowel syndrome:current under-standing [J]. Recenti Prog Med,2007,98(11):543-547
[38]Lucas A, Cobelens PM, Kavelaars A, et al. Disturbed in vitro adren-ergic modulation of cytokine production in inflammatory bowel diseases in remission [J]. Neuroimmunol, 2007,182(1-2):195-203
[39]雷景根.盐酸帕罗西汀治疗肠易激综合征34例临床观察[J].浙江预防医学.2006,18(8)：66-67.
[40]谢启文.神经肽[M].上海：复旦大学出版社,2004,167-169.
[41]林琳,赵志泉,殷咏梅,等.胃肠激素与肠易激综合征的相关性[J].江苏医药杂志,2001,27(3)：166-168.
[42]林琳,赵志泉,殷咏梅,等.胃肠激素与肠易激综合征的相关性[J].江苏医药杂志,2001,27(3)：166-168.
[43]Husum H,Van Kammen D,Termeer E, et al.Neuropsychopharmacology,2003,9:422-428.
[44]Chiocchetti R,PooleDP, KimuraH,et al.Cell Tissue Res,2003,311:11-22
[45]Michalkiewicz M,Zhao G, Jia Z,et al. Central neuropeptide Y signaling ameliorates N(omega)-nitro-L-arginine methyl ester hypertension in the rat through a Y1 receptor mechanism[J].Hypertension,2005,45(4):780-785.
[46]Michalkiewicz M,Knestaut KM,Bytchkova EY,et al.Hypotension and reduced catecholamines in neuropeptide Y transgenic rats[J]. Hypertension,2003,41(5):1056-1062.
[47]Kokare DM,Dandekar MP,Chopde CT,Subhedar N.Interaction between neuropeptide Y and alpha-melanocyte stimulating hormone in amygdale regulates anxiety in rats.Brain Res.2005 May 10;1043(1-2):107-14
[48]李继锋,王舟,纪祥瑞.降钙素基因相关肽在神经免疫内分泌网络中的作用[J].中国神经免疫学和神经病学杂志,2004,1(3)：177-179.
[49]陈军,李静.头针治疗偏头痛的疗效及对CGRP、NO的调节[J].上海针灸杂志,2008,27(12)：6-7.
[50]刘海涛,彭旭,吴曙粤.血浆内皮素及降钙素基因相关肽的研究进展[J],内科,2006,1(2)：173-174.
[51]郑雪雁.实验性慢性结肠炎症大鼠的内脏感觉变化及帕罗西汀的影响[D].福建医科大学,2007：27-28.
[52]张峰,袁川评,柳巨雄.SP和CGRP在炎症性肠病大鼠结肠中的表达规律[J].中国比较医学杂志,2011,21(12)：26-30.
[53]陈吉,邓展生,张阳德.肠易激综合患者结肠黏膜VIP.CGRP和肥大细胞变化及相关性研究[J],中国现代医学杂志,2011,21(8)：934-937.
[54]Parlapiano C, Paoletti V, Campana E, et al. CGRP and ET-1 plasma levels in normal subjects[J].Eur Rev Med Pharmacol Sci,1999;3(3):139-141.
[55]Keith IM. The role of endogenous lung neuropeptides in regulation of the pulmonary circulation[J].Physiol Res,2000;49(5):519-537.
[56]Wang Hai-yun, Shi Jun-jie. Effect of Xiaoyaosan on the ET Levels in Serum of Stressed IBS Rats[J]. Chinese Archives of Traditional Chinese Medicine,2009,27(11):2406-2408
[57]杨磊,赵雅芳,李滢.电针不同穴位对肠易激综合征模型大鼠血浆CGRP、VIP、ET含量的影响[J]吉林中医药,2011,31(12)：1235-1237.
[58]陈言言,王立,董宏生.肠易激综合征及溃疡型结肠炎患者胃肠激素和细胞因子的变化[J].中国中西医结合消化杂志,2003,11(6)：370-371.
[59]Van Geldre LA,Lefebvre RA.Interaction of NO and VIPin gastrointestinal smooth muscle relaxation[J].CurrPharm Des,2004,10(20):2483-2497.
[60]Evangelista S.Involvement of tachykinins in intestinal inflammation[J].Curr Pharm Des,2001,7(1):19-30.
[61]Wu HG,Jiang B,Zhou EH,et al.Regulatory Mechanism of Electro-acupuncture in Irritable Bowel Syndrome:Preventing MC Activation and Decreasing SP VIP Secretion[J].Dig Dis Sci,2007,13
[62]李兆申,董文珠,邹多武,等.肠易激综合征肠黏膜SP、VIP、CGRP变化的研究[J],第二军医大学学报,2003,24(2)：147-150.
[63]梁荣新,蔡联英,郑琴芳.肠易激综合征患者乙状结肠动力及胃肠激素变化的研究[J].广西医科大学学报,2003,20(6)：843-845.
[64]林琳,赵志泉,殷咏梅,等.胃肠激素与肠易激综合征的相关性[J].江苏医药,2001,27(3)：166-8.
[65]张军,张振玉,孙丹莉.肠易激综合征患者血浆胃肠激素含量的变化,诊断学理论与实践,2006,5(1)：58-59.
[66]陈敏.TNF-α及PGE2与小儿全身炎症反应综合征[J].内蒙古医学杂志Inner MongoliaMed J,2012,44(10),1209-1210.
[67]应延风,陈浩浩,金耀建,等.PGE2对气道平滑肌细胞分泌和凋亡影响的研究[J／CD].中华哮喘杂志：2012,6(4)：239-244.
[68]秦明,黄裕新,王景杰,等.电针在应激大鼠胃粘膜保护作用中对氧自由基和前列腺素的影响[J].第四军医大学学报(J FourthM ilM ed U niv),2001; 22 (9):803-805
[69]L i T, Zhang XJ. The injurious effect of oxygen radicals on the membrance of gastric muco sal cells[J].Beijing Yike Daxue Xuebao (J B eijing Med Univ),1993,25(5):321-323.
[70]Pavies GR,SimmondsNJ,Stevens TR, Granison A, Black DR,Ramp ton DS. M uco sal reactive oxygen metabo lite p roduction in duodenal ulcer disease [J]. Gut,1992; 33 (12): 1467-1472.
[71]L i T, Zhang XJ. Role of oxygen2derived free radicals in stress induced gastric ulceration [J]. Sheng li Xuebao (A cta P hy siolog ica S inica),1993; 45 (3):286-291.
[72]Dong XY, Sen ZS. A p relim inary study on themechanism of resistance of gastric muco sa to the second injury in the rat [J].Beijing Yike Daxue Xuebao (J B eijing Med Univ), 1995; 27(1):9-11.
[73]Konturek PK, Brzozow sk i T, Konturek S, Dembinsk iA. Ro leofepidermal growth facto r, prostaglandin, and sulfhydrylsin stress2induced gastric lesions [J]. Gastroenterology, 1990; 99:1607-1615.
[74]L i T, Zhang XJ. The relationsh ip between sulfhydryls and the protective effect of p rostaglandin E2 and somatostatin on gastricmucosa [J]. B eijing YikeDaxue Xuebao (J Beijing Med Univ),1989; 21 (6):476-478.
[75]Zhao BX, Guo CJ, Yin KJ. Effects of acupuncture on oxygen free radicals andultrastructure in stress rats [J]. Shanghai Zhenjiu Zazhi (JS hanghai Acupunct),1995; 14: 235-236.
[76]Fosslien E. Adverse effects of nonsteroidalanti inflammatory drugs on the gastro intestinal system [J]. Ann Clin Lab Sci,1998:28 (1):67-81.
[77]Sun B, L i ZS. N SA ID induced gastric muco sal damage and its prevention in rats [J]. Shijie Huaren Xiaohua Zazhi (World Chin J Digestol),2000; 8 (3):273-275.
[78]A i BW, Guo CJ, Yin KJ. The effects of acupuncture on prostaglandin in the gastric mucosa in experimeatal gastric ulcerrats [J]. S hanghai Zhenjiu Zaz i (J S hanghai Acupunct),1995; 14:235-236.
[79]蓝升红,周丽萍,屠金夫,等.前列腺素E2对重症胰腺炎大鼠血清肿瘤坏死因子及白介-6的调控作用[J.肝胆胰外科杂志,2001,13(2)：67-69.
[80]薛瑞,苗一非,杨吉春,等.前列腺E2对免疫细胞及炎症相关疾病的调控作用[J].生理科学进展,2011,42(3)：165-168.
[81]邱春兰,王兴勇.全身炎症反应综合征发病机制研究进展[J].西部医学,2009,21(4)：662-663.
[1]陈文杰,田牛主编.微循环的理论与应用[M].北京：人民卫生出版社,1987：23.
[2]田牛.微循环学[M].北京：原子能出版社,2004：40.
[3]于金萍,冷贵生,郭述苏,等.脑梗塞患者红细胞膜微粘度与球结膜微循环之关系[J].微循环学杂志,1996,6(1)：38.
[4]陈邦国.针刺治疗脑血栓形成的微循环观察[J],上海针灸杂志,1997,16(2)：13.
[5]周杰芳,靳瑞.针刺对中风患者微循环与血液流变学作用的观察,广州中医学院学报,1991,8(2)：196.
[6]吴焕金,高镇五,张琳珊.隔药饼灸对慢性非特异性结肠炎患者球结膜微循环的影响[J].针灸学报,1991,(3)：4-6.
[7]李湘.运动性头针对糖尿病视网膜病变患者眼部血流动力学的影响[J].山东医药,2006,46(16)：43-44.
[8]唐照亮.艾灸对血瘀证活血化瘀作用的研讨[J].安徽中医学院学报,2007,26(1)：21-24.
[9]赵雪梅,刘育英,等.100例健康人不同部位体表血流量的测量[J].微循环学杂志,2004,14(2)：43-45.
[10]张栋,王淑友,等.激光多普勒成像技术对针灸效果的观察[J].上海针灸杂志,2004,23(5)：37-40.
[11]张栋,马慧敏,等.激光多普勒成像技术对体表不同部位微血流图像的初步观察[J].中国微循环,2004,8(5)：283-285.
[12]李丽,龚剑秋,丁光宏,等.头排针治疗脑梗死过程中颈动脉血液动力学能量变化研究[J].针刺研究,2009,34(5)：334-338.
[13]胡青华,庄逢源.荧光显微镜观察电针对实验性急性出血性低血压脑微循环的作用[J].微循环学杂志,2000,10(1)：18-20.
[14]韩冰冰,王世军,蒋继强.电针对MC AO大鼠海马微循环血流量的影响[J].时珍国医国药,2010,10,21(3)：730-731.
[15]喻斌,孙永,吕高红,等.电针配合贯叶连翘提取物对抑郁大鼠行为学及脑微循环的影响[J].针刺研究,2011,36(3)：164-170.
[16]卢岩,王世军.电针对局灶性脑缺血大鼠脑葡萄糖代谢的影响[J.]辽宁中医杂志,2011,38(5)：995-999.
[17]马莎,陈祥芳,等.针刺对偏头痛大鼠硬脑膜微循环的影响[J].吉林中医药,2008,28(8)：610-611.
[18]Komori M, Takada K, Tomizawa Y, et, Microcirculatory responses to acupuncture stimulation and phototherapy[J]. Anesth nalg,2009,108(2):635-640.
[19]陈邦国.针刺治疗脑血栓形成的微循环观察[J].上海针灸杂志,1997,16(2)：13.
[20]李信明,魏安宁,李定明.“三联疗法”治疗周围性面神经麻痹及其对面部微循环血流量的影响[J].中华物理医学与康复杂志,2006,28(8)：549-550.
[21]刘雅静,张伯儒.缪刺结合刺络拔罐法治疗顽固性面神经麻痹临床研究[J].吉林中医药,2008,28(1)：46-47.
[22]李明珍,吴人照.电针强刺激对小白鼠耳廓微循环的影响[J].湖北中医杂志,1989(6)：43-44.
[23]阴怀清,王阿琚,范庆,等.脑性瘫痪患儿头针治疗前后耳廓微循环变化的观察[J].山西医药杂志,1992,21(6)：352.
[24]郑重,宋开源,胡晓梅,等.电针改善VBI所致内耳微循环障碍及对VOR的影响[J].中国应用生理学杂志,2006,22(1)：99-104.
[25]刘俊岭,曹庆淑,罗明富,等.电针心包经经穴改善急性心肌缺血机理的研究[J].针刺研究,1999,24(4)：282-287.
[26]罗明富,曹庆淑,刘俊岭,等.电针“内关”穴对急性心肌缺血区微血管作用的透射电镜观察[J].针刺研究,1995,21(4)：11-14.
[27]罗明富,王平,刘俊岭,等.电针“内关”穴对急性心肌缺血区肌原纤维、线粒体及血小板的影响[J].针刺研究,2001,26(2)：119-121.
[28]李洁.电针对实验性急性心肌缺血影响的研究[J].上海针灸杂志,1999,18(2)：44-47.
[29]Bhatia M,Neoptolemos JP,Slacin J.Inflammatory mediators as therapeutic targets in acute pancreatitis.Curr Opin Invest Drugs,2001,2(4):496-501.
[30]陈友岱,周总光.缓激肽在器官微循环损伤中的作用[J].微循环学杂志,2001,11(1)：43-46.
[31]王强,闫军.低相对分子质量肝素改善急性胰腺炎微循环的研究[J].中国药物与临床,2011,11(3)：294-295.
[32]吴富东,王世军,王晓燕,等.电针足三里穴对正常大鼠微循环的调节作用及穴位脏腑相关性研究[J].中医杂志,2010,51(12)：1101-1103.
[33]胡森,王磊,周洁平.电针足三里对烫伤休克大鼠肠黏膜血流和微血管通透性的影响[J]微循环学杂志,2010,20(1)：10-12.
[34]赵雅芳,李春华,嵇╲波,等.电针三阴交、血海对痛经模型大鼠子宫微循环的影响[J].微循环学杂志,2011,21(2)：4-7.
[35]李春华,嵇波,赵雅芳,等.电针预先介入对痛经模型大鼠子宫微循环的影响[J].中华中医药杂志,2010,25(12)：1979-1984.
[36]杨磊,李滢,赵雅芳,等.电针不同穴位对肠易激综合征模型大鼠内脏敏感性及肠系膜微循环的影响[J].微循环学杂志,2011,21(4)：4-6.
[37]康新,卓鹰.电针不同穴位对家兔微循环障碍影响的初步研究[J].中国血液流变杂志,2007,17(3)：491-492.
[38]王诚宏.腰部银质针针刺与下肢微循环变化的研究[J].中国疗养医学,2004,13(3)：134-135.
[39]白良川,许冠荪,张荣军,等.电针对兔激素性股骨头坏死局部组织血流量的影响[J].安徽中医学院学报,2004,23(1)：33-34.
[40]朱柏君,王援朝,徐文斌.电针对急性实验性关节炎大鼠外周微循环的影响[J].针刺研究,1993,18(3)：219-222.
[41]陈军,李静.头针治疗偏头痛的疗效及对CGRP. NO的调节[J].上海针灸杂志,2008,27(12)：6-7.
[42]刘海涛,彭旭,吴曙粤.血浆内皮素及降钙素基因相关肽的研究进展[J],内科,2006,1(2)：173-174.
[43]Parlapiano C, Paoletti V, Campana E, et al. CGRP and ET-1 plasma levels in normal subjects[J].Eur Rev Med Pharmacol Sci,1999;3(3):139-141.
[44]Keith IM. The role of endogenous lung neuropeptides in regulation of the pulmonary circulation[J].Physiol Res,2000;49(5):519-537.
[45]杨磊,赵雅芳,李滢.电针不同穴位对肠易激综合征模型大鼠血浆CGRP、VIP、ET含量的影响[J].吉林中医药,2011,31(12)：1235-1237.
[46]陈言言,王立,董宏生.肠易激综合征及溃疡型结肠炎患者胃肠激素和细胞因子的变化[J].中国中西医结合消化杂志,2003,11(6)：370-371.
[47]Van Geldre LA,Lefebvre RA. Interaction of NO and VIPin gastrointestinal smooth muscle relaxation[J].CurrPharm Des,2004,10(20):2483-2497.
[48]Evangelista S.Involvement of tachykinins in intestinal inflammation[J].Curr Pharm Des,2001,7(1):19-30.
[49]Wu HG, Jiang B, Zhou EH, et al Regulatory Mechanism of Electro-acupuncture in Irritable Bowel Syndrome:Preventing MC Activation and Decreasing SP VIP Secretion[J]. Dig Dis Sci,2007,13 [Epub ahead of print].
[50]李兆申,董文珠,邹多武,等.肠易激综合征肠黏膜SP、VIP、CGRP变化的研究[J].第二军医大学学报,2003,24(2)：147-150.
[51]梁荣新,蔡联英,郑琴芳.肠易激综合征患者乙状结肠动力及胃肠激素变化的研究,广西医科大学学报,2003,20(6)：843-845.
[52]林琳,赵志泉,殷咏梅,等.胃肠激素与肠易激综合征的相关性[J].江苏医药,2001,27(3)：166-8.
[53]陈敏,TNF-α及PGE2与小儿全身炎症反应综合征[J].内蒙古医学杂志Inner Mongolia Med J 2012,44(10),1209-1210.
[54]应延风,陈浩浩,金耀建,等.PGE2对气道平滑肌细胞分泌和凋亡影响的研究[J/CD].中华哮喘杂志,2012,6(4)：239-244.
[55]林琳,赵志泉,殷咏梅,等.胃肠激素与肠易激综合征的相关性[J].江苏医药杂志,2001,27(3)：166-168.
[56]Husum H,Van Kammen D,Termeer E, et al. Neuropsychopharmacology,2003,9:422-428.
[57]Chiocchetti R,PooleDP,KimuraH,et al. Cell Tissue Res,2003,311:11-22
[1]张声生,李乾构,魏玮,等.肠易激综合征中医诊疗共识意见[J].中华中医药杂志,2010,25：1062-1065.
[2]Longstreth GF, Thompson WG, Chey WD, et al. Functional bowel disorders. Gastroenterology,2006,130:1480-1419.
[3]Spinelli A. Irritable bowel syndrome. Clin Drug Investig,2007; 27:15-33.
[4]Xiong LS, Chen MH, Chen HX, Xu AG, Wang WA,Hu PJ. A population-based epidemiologic study of irritable bowel syndrome in South China:stratifi ed randomized study by cluster sampling. Aliment Pharmacol Ther 2004; 19:1217-1224.
[5]张万岱.功能性胃肠病罗马标准的简介和解读[J.]世界华人消化杂志.2008,16：120-124.
[6]张文静.成人肠易激综合征诊治新指南[J].现代消化及介入诊疗.2007,12：141.
[7]孙菁,袁耀宗.肠易激综合征：罗马III标准与我国共识意见解读[J].医学与哲学·临床决策论坛版.2008,29：12-16.
[8]Williams CL,Villar RG,Peterson JM,Burks TF.Stress-induced changes in intestinal transit in the rat:a model for irritable bowel syndrome.Gastroenterology,1988,94:611-621.
[9]吕红,王伟岸,钱家鸣.传统束缚应激动物模型内脏感觉的新评价[J].胃肠病学和肝病学杂志,2005,14：111-113.
[10]吴晓亮,孙建华.肠易激综合征国内实验研究现状的调查[J].世界华人消化杂志.2010,18(35)：3818-3823.
[11]任天华,胡品津,胡志远,等.新生期母婴分离对大鼠成年后结直肠扩张后5-羟色胺表达的影响[J].中山大学学报,2004,25(3)：234.
[12]刘雁冰,袁耀宗,陶然君,等.大鼠肠道高敏性模型的建立及其内脏敏感性评估[J].中华消化杂志,2003,23(1)：34-37.
[13]Al Chaer ED, Kawasaki M, Pasricha P J. A new of chronicvis ceralhy persensitivity in adult rats induced by colon irritation during postnatal development [J]. Gastroenterology, 2000,119(5):1276.
[14]李延青,杨军.肠易激综合征内脏感觉过敏动物模型的建立[J].胃肠病学和肝病学杂志,2003,12(4)：332.
[15]彭丽华,孙刚,杨云生.便秘型肠易激综合征新概念模型的建立.世界华人消化杂志,2004,12：112-116.
[16]La JH, Kim TW, Sung TS, et al. Visceral hypersensitivity and altered colonic motility after subsidence of inflammation in a rat model of colitis [J]. World J Gastroenterol, 2003,9:2791-2795.
[17]O'Mahony SM,Marchesi JR,Scully P,Codling C,Ceolho AM,Quigley EM,Cryan JF,Dinan TG. Early life stress alters behavior,immunity,and microbiota in rats:implications for irritable bowel syndrome and psychiatric illnesses.Biol Psychiatry2009;65:263-267.
[18]WILLIAMS C L, VILLAR R G, PETERSON J M, et al. Stress- induced changes in intestinal transit in the rat:a model for irritable bowel syndrome[J]. Gastroenterology,1988, 94:611.
[19]LI Z D, LI D G, WU J C,et al.Effects and mechanism of CRF microinjection into PVN on gastric emptying[J]. J SSMU,2000,12:103.
[20]刘清华,李定国.肠易激综合征动物模型复制思路和方法[J].国外医学：消化系疾病分册,2002,40：154-156.
[21]Collions SM, McHugh K, Jacobson K, et al. Prerious inflammation alters the response of the rat colon to stress. Gastroenterology,1996,111:1509-1515.
[22]郭敏,李延青,于秀娟,孔庆莲等.大鼠急性肠道感染后肠功能紊乱动物模型的建立.山东大学学报(医学版),2006,44(6)：586-589.
[23]王伟岸,钱家鸣,潘国宗.肠易激综合征的发病机制[J].胃肠病学和肝病学杂志,2001,10：103-105.
[24]刘清华.肠易激综合征动物模型复制思路及方法[J].国外医学(消化系疾病分册),2002,22：154-156.
[25]彭丽华,杨云生,孙刚,等.便秘型肠易激综合征新概念模型的建立[J].世界华人消化杂志,2004,12：112-116.
[26]Coutinho S, Plotsky PM, SabladM,etal. Neonatalmater-nal separation alters stress-induced response to visceroso-matic nociceptive stimuli in rats[J]. Am J PhysiolGstroi-ntestLiverPhysio,2002,282:307-316.
[27]O'Mahony SM, Marchesi JR, Scully P, Codling C, Ceolho AM, Quigley EM, Cryan JF, Dinan TG. Early life stress alters behavior, immunity, and microbiota in rats:implications for irritable bowel syndrome and psychiatric illnesses. Biol Psychiatry 2009; 65:263-267.
[28]朱力阳,彭成,谢晓芳,王嫣虹.化学刺激引起的两种大鼠内脏高敏感性模型的建立[J].中国药理学通报2010；26：267-270.
[29]徐俊荣,罗金燕.肠易激综合征动物模型研究进展[J].基础医学与临床,2006,26(8)：902-904.
[30]Collins SM, Mchugh K, Jacobson K,et al. Previous in-flammation alters the response of the rat colon to stress[J]. Gastroenterology,1996,111:1509-1515.
[31]Smith JL,Bayles D.Postinfectious irritable bowel syndrome:a longterm consequence of bacterial gastroenteritis.J Food Prot,2007,70:1762-1769.
[32]De Giorgio R,Barbara G.Is irritable bowel syndrome an inflammatory disorder?Curr Gastroenterol Rep,2008,10:385-390.
[33]Barbara G, Vallance BA, Collins SM. Persistent intestinal neuron muscular dysfunction after acute nematode infection in mice. Gastroenterology,1997,113:12242-12321.
[34]Toulouse M, Coelho AM, Fioramonti J, et al. Role of tachykinin NK2 receptors in normal and altered rectal sensitity in rats. Br J Pharmacol,2000,129:193-199.
[35]朱力阳,彭成,谢晓芳,等.化学刺激引起的两种大鼠内脏高敏感性模型的建立[J].中国药理学通报.2010,26(2)：267-270.
[36]Al Chaer ED, Kawasaki M, Pasricha P J. A new of chronicvis ceralhy persensitivity in adult rats induced by colon irritation during postnatal development [J]. Gastroenterology, 2000,119(5):1276.
[37]刘雁冰,袁耀宗,陶然君,等.大鼠肠道高敏性模型的建立及其内脏敏感性评估[J].中华消化杂志,2003,23(1)：34-37.
[38]Lomax AE, Fernandez E, Sharkey KA. Plasticity of the enteric nervous system during intestinal inflammation[J]. Neurogastroenterol Motil,2005,17(1):4-15.
[39]Tornblom H, Lindberg G, Nyberg B, Veress B. Full-thickness biopsy of the jejunum reveals inflammation and enteric neuropathy in irritable bowel syndrome. Gastroenterology 2002; 123:1972-1979.
[40]Vasina V, Barbara G, Talamont L, et al.Enteric neuroplasticity evoked by inflammation[J].Auton Neurosci,2006,30(126-127):264-272.
[41]朱雪萍,陈卫昌,朱晓黎,等.脑肠肽、脑肠轴在肠易激综合征模型中作用机制研究[J].临床儿科杂志2008,26：236-239.
[42]王伟岸,钱家鸣,潘国宗,等.脑-肠互动指向性条件应激肠易激综合征动物模型的建立[J].中华消化杂志,2004；24：590-593
[43]Lu H, Qian JM, Jin GL, Zhou DF, Shen YC. [Theestablishment of an animal model of gut-brain interaction in irritable bowel syndrome for the evaluation of visceral sensation, motility and psychological behavior.] Zhonghua Neike Zazhi 2009; 48:1035-1039
[44]吴晓亮,孙建华.肠易激综合征国内实验研究现状的调查[J],世界华人消化杂志,2010,18(35)：3818-3823.
[45]Al-chaer ED,Kawasaki M,Pasricha PJ.A new model of chronic visceral hypersensitivity in adult rats induced by colon irritation during postnatal development [J].Gastroenterology,2000,119:1276-1285.
[46]Mayer EA,Gebhart GF.Basic and clinical aspects of visceral hyperalgesia[J].Gastroenterology,1994,107:271-293.
[47]Soderholm JD, Yates DA, Gareau MG, et al. Neonatal maternal separation predisposes adult rats to colonic barrier dysfunction in response to mild stress[J]. Am J Physiol Gastrointest Liver Physiol,2002,283:1257-1263.
[48]蔡淦,张正利.电刺激-束缚大鼠肠易激综合征模型的应用与评价[J].中国中西医结合消化杂志,2005,13(2)：75-77.
[49]刘新光.模拟肠易激综合征动物动物模型研究的评述[J].胃肠病学和肝病学杂志,2004,12：329-330.
[50]桂先勇.寒冷-束缚应激对大鼠结肠运动的后续效应[J].中华消化杂志,1997,17(2)：94-96.
[51]Eutamene H,Bradesi S,Larauche M,Theodorou V,Beaufrand C,Ohning G,Fioramonti J,Cohen M, Bryant AP.Kurtz C,Currie MG,Mayer EA,Bueno L.Guanylate cyclase C-mediated antinociceptive effects of linaclotide in rodent models of visceral pain. Neurogastroenterol Motil 2010;22:312-384.
[52]李冬华,李春淼,李伍善,邹志东,张炎.痛泻要方对肠易激综合征模型大鼠血管活性肠肽的影响.时珍国医国药2007；18：2098-2099
[53]费晓燕,谢建群,郑昱,吴大正,袁建业.疏肝饮对腹泻型肠易激综合征模型大鼠胃动素和胆囊收缩素的影响.上海中医药杂志2008；42：63-65
[54]李丹,吕妍,唐方.腹泻型肠易激综合征大鼠模型的建立[J].天津中医药.2009,26(3)：240-242.
[55]白殿卿,孙晓宁,安钰,郑颖.大鼠肠易激综合征模型的建立及其评价.中国实验动物学报,2009,17(5)：377-379.
[56]安钰,白殿卿,付健,等.乙酸灌肠加束缚应激致大鼠肠易激综合征模型的建立及其评价[J].世界华人消化杂志2009；17：1548-1551.
[57]王伟岸,钱家鸣,潘国宗,等.脑-肠互动指向性条件应激肠易激综合征动物模型的建立[J].中华消化杂志,2004,24(10)：590-593.
[58]徐海珍,谢建群,施斌,等.腹泻型肠易激综合征大鼠结肠黏膜炎性细胞因子的表达及温中健脾方对其影响的研究[J].上海中医药杂志,2007,41(6)：69.
[59]石君杰,王海云,戴玉英,等.逍遥散对肠易激综合征大鼠腹壁肌电活动紊乱调节作用的机制探讨[J].浙江中医杂志,2008,43(3)：139.
[60]赵文娟,王垂杰.腹泻型肠易激综合征大鼠模型的建立[J].国际中医中药杂志,2008,30(1)：16.
[61]胡萍萍,吕宾,范一宏.内脏高敏感大鼠脑部5-羟色胺表达差异的研究[J].中华消化杂志,2006,26(5)：347.
[62]Wingate D,Hongo M, Kellow J,et al.Disorders of gastrointestinal motility:towards a new classification.J Gastroenterol Hepatol.2002,17 (Suppl):S1-14.
[63]Kalantar JS, Locke GR, Zinsmeister AR, et al. Familial agg-regation of irritable bowel syndrome:a prospective study[J]. Gut,2003,52:1703-1707.
[64]罗小雨,钟良.肠易激综合征发病机制的研究进展[J],国际消化病杂志,2010,30(6)：321-324.
[65]Sinhamahapatra P, Saha SP, Chowdhury A,et al. Visceral afferent hypersensitivity in irritable bowel syndrome evaluation by cerebral evoked potential after rectal stimulation[J]. Am J Gastroenterol,2001,96(7):2150-2157.
[66]Nozu T,KudairaM.Altered rectal sensory response induced by balloon distention in patients with functional abdominal pain syndrome[J]. Biopsychosoc Med,2009,3:13.
[67]张鸣鸣,姜敏.肠易激综合征发病机制研究进展[J].世界华人消化杂志,2009,17：2484-2490.
[68]ChadwickVS, ChenW, Shu D,etal. Activation of the mucosal im-mune system in irritable bowel syndrome[J]. Gastroenterology,2002,122(7):1778-1783.
[69]PimentelM, Chow EJ, Lin HC. Normalization of lactulose breathtesting correlateswith symptom improvement in irritable bowe syn-drome:a double-blind, randomized, placebo-controlled study[J].Am JGastroentero,1 2003,98(2):412-419.
[70]谢启文.神经肽[M].上海：复旦大学出版社,2004,167-169.
[71]李继锋,王舟,纪祥瑞.降钙素基因相关肽在神经免疫内分泌网络中的作用[J].中国神经免疫学和神经病学杂志,2004,1(3)：177-179.
[72]Keith IM. The role of endogenous lung neuropeptides in regulation of the pulmonary circulation[J].Physiol Res,2000;49(5):519-537.
[73]Van Geldre LA,Lefebvre RA. Interaction of NO and VIPin gastrointestinal smooth muscle relaxation[J].CurrPharm Des,2004,10(20):2483-2497.
[74]Evangelista S.Involvement of tachykinins in intestinal inflammation[J].Curr Pharm Des,2001,7(1):19-30.
[75]陈敏TNF-a及PGE2与小儿全身炎症反应综合征[J].内蒙古医学杂志Inner Mongolia M ed J 2012,44(10):1209-1210.
[76]陶琳,柯美云,王智凤.小肠细菌过度生长与功能性胃肠疾病的关系[J].中国中西医结合消化杂志,2005,13(2)：122-123.
[77]Portincasa P, Moschetta A, Baldassarre G, Altomare DF, Palasciano G. Pan-enteric dysmotility,impaired quality of life and alexithymia in a large group of patients meeting ROME II criteria for irritable bowel syndrome[J]. World J Gastroenterol,2003;9:2293-2299.
[78]Small PK, Loudon MA, Hau CM, Noor N, Campbell FC. Large-scale ambulatory study of postprandial jejunal motility in irritable bowel syndrome[J]. Scand J Gastroenterol, 1997;32:39-47.
[79]AL-Chaer E D, Kawasaki M, Pasricha P J. A new model of chronic visceral hypersensitivity in adult rats induced by colon irritation during postnatal development [J]. Gastroenterology,2000,119:1276-1285.
[80]刘雁冰,袁耀宗,陶然君,等.大鼠肠道高敏感性模型的建立及其内脏敏感性评估[J].中华消化杂志,2003,23：34-37.
[81]李延青,杨军.肠易激综合征内脏感觉过敏动物模型的建立[J].胃肠病学和肝病学杂志,2003,12(4)：332.
[82]白殿卿,孙晓宁,安钰,等.大鼠肠易激综合征模型的建立及其评价[J].中国实验动物学报,2009,17(5)：377-379.
[83]郭玉婷,李延青,杨军,等.肠易激综合征内脏过敏大鼠脊髓诱发电位的研究[J].胃肠病学,2004,9(6)：347.
[84]李兆申.肠易激综合征内脏高敏感性研究现状和展望.第二军医大学学报.2003；24(2)：120-123.
[1]张声生,李乾构,魏玮,等.肠易激综合征中医诊疗共识意见[J].中华中医药杂志,2010,25：1062-1065.
[2]Longstreth GF, Thompson WG, Chey WD, et al. Functional bowel disorders. Gastroenterology,2006,130:1480-1419.
[3]赵雅芳,李春华,稽波,等.电针三阴交、血海对痛经模型大鼠子宫微循环的影响[J].微循环杂志,2011,21(2)：4-7.
[3]ZhaoYa-fang, Li Chun-hua, Ji Bo, et al. Effect of electroacupuncture at Sanyinjiao and Xuehai on uterine miocrocirculation in dysmenorrhea Rats[J]. Chinese Journal of Microcirculation,2011,21(2):4-7.
[4]任晓暄,郭孟玮,赵雅芳,等.电针对大鼠类痛经反应、脊髓K-受体表达及中脑导水管周围灰质脑啡肽和p-内啡肽含量的影响[J].针刺研究,2012,37(1)：1-7.
[4]Ren Xiao-xuan,Guo Meng-wei, Zhao Ya-fang, et al. Effect of electroacupuncture on pain reactions, expression of spinal κ-opioid receptor and contents of enkephalin and (3-endorphin in periaqueductal gray of midbrain in dysmenorrheal model rats[J]. Acupuncture Research, 2012,37(1):1-7
[5]AL-Chaer E D,Kawasaki M,Pasricha P J.A new model of chronic visceral hypersensitivity in adult rats induced by colon irritation during postnatal development [J].Gastroenterology, 2000,119:1276-1285.
[6]刘雁冰,袁耀宗,等.大鼠肠道高敏感性模型的建立及其内脏敏感性评估[J].中国消化杂志,2003,23：34-37.
[7]Al-chaer ED, Kawasaki M, Pasricha PJ. A new model of chronic visceral hypersensitivity in adult rats induced by colon irritation dur-ing postnatal development. Gastroenterology, 2000,119(115):276-1285.
[8]Mayer EA,Collins SM. Evolving PathoPhysiologic models of functional gastrointestinal disorders [J]. Gastroenterology,2002,122:2032-2048.
[9]王伟岸,钱家鸣,潘国宗,等.脑-肠互动指向性条件应激肠易激综合征动物模型的建立[J].中华消化杂志,2004,24：590-593.
[10]曾昭炜,谢忠明,唐荣福等.观察动物微循环的实验方法[J]微循环学杂志,2011,21(3)：26-32.
[11]田牛.微循环方法学[M].北京：原子能出版社,1993：175.
[12]AL-Chaer E D, Kawasaki M, Pasricha P J. A new model of chronic visceral hypersensitivity in adult rats induced by colon irritation during postnatal development [J]. Gastroenterology,2000,119:1276-1285.
[13]Bouin M, Plourde V, Boivin M, et al. Rectal distention testing in patients with irritable bowel syndrome:sensitivity, specificity, andpredictive values of pain sensory thresholds. Gastroenterology,2002,122(7):1771-1777.
[14]袁耀宗,刘雁冰,陶然君等.肥大细胞在应激所致大鼠直肠高敏感性中的作用[J].中华消化杂志,2003,23(12)：727-730.
[15]赵宁侠,郭瑞林,任秦有.针灸治疗原发性痛经临床疗效及血液流变学相关性分析[J].浙江中医药大学学报,2007,31(3)：364-367.
[16]周慧敏,张庆富,张晓琳.肠系膜微循环研究的若干进展.中国微循环,2006,10(3)：217-219.
[17]赵雅芳,李春华,嵇波,等.电针三阴交、血海对痛经模型大鼠子宫微循环的影响[J].微循环学杂志,2011,21(2)：4-7.
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