湿疹纳米乳膏成分对淋巴细胞和HaCaT细胞共培养的实验研究
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摘要
背景和目的：
慢性湿疹作为临床一种常见病、多发病,其病因复杂、皮损多样、反复发作、剧烈瘙痒、治疗棘手,严重影响着患者的身心健康。多数研究结果证实慢性湿疹患者Thl/Th2细胞分化失衡引起的细胞因子分泌异常影响着湿疹的发生发展过程,慢性湿疹患者皮损研究提示T淋巴细胞浸润在慢性湿疹发病机制中有重要作用,活化的T细胞诱导角质形成细胞的凋亡为湿疹慢性化的转折点,阻断角质形成细胞的凋亡可以减轻湿疹慢性化进程的发生。HaCaT细胞株保持有正常人角质形成细胞的分化和增殖特性,为通用的研究角质形成细胞生理功能的模型,探索采用淋巴细胞和HaCaT细胞共培养来建立慢性湿疹的体外细胞模型,以进行慢性湿疹的药效学实验和药物筛选实验具有重要意义。
湿疹纳米乳膏为导师杨志波教授在多年临床经验总结的基础上,结合现代纳米技术研制的治疗慢性湿疹的外用制剂,前期临床研究证实疗效确切。本课题拟探索进行淋巴细胞和HaCaT细胞共培养的实验研究,观察湿疹纳米乳膏成分对慢性湿疹体外细胞模型HaCaT细胞的凋亡率,研究慢性湿疹体外细胞模型的建立及湿疹纳米乳膏成分治疗慢性湿疹的作用机制,为慢性湿疹体外细胞模型的建立及临床用药提供实验依据。
方法：
通过正交设计实验探索建立淋巴细胞和HaCaT细胞共培养的最佳凋亡模型,模拟慢性湿疹体外细胞的部分发病机制,采用MTT实验观察湿疹纳米乳膏主要成分对HaCaT细胞活力影响的浓度,流式细胞仪和TUNEL法检测药物干预细胞共培养模型后HaCaT细胞的凋亡率。
结果：
慢性湿疹患者淋巴细胞与HaCaT细胞以1：1、1：3、3：1的比例接种分别共培养24h、48h、72h,淋巴细胞均可诱导HaCaT细胞凋亡；淋巴细胞和HaCaT细胞以共培养72h,接种比例Lymphocyte:HaCaT=3:1为最佳共培养凋亡模型；Mat在50ug/mL及以下浓度对HaCaT细胞生长无明显抑制作用,12.5ug/mL浓度对共培养凋亡模型有促进作用,25ug/mL浓度逆转作用不明显,50ug/mL浓度逆转作用明显。Oxy在50ug/mL及以下浓度对HaCaT细胞活力无影响,12.5ug/mL浓度对共培养凋亡模型有促进作用,25ug/mL.50ug/mL浓度均有逆转作用。Oxy对共培养凋亡模型的促进、逆转作用均较Mat强；Ber在1.5mg/L浓度对HaCaT细胞的增殖无明显影响,Ber对共培养凋亡模型有逆转作用并与Ber浓度呈剂量依赖性；Pae在15.625mg/L的浓度对HaCaT细胞的增殖无明显影响,75mg/L.15mg/L对共培养凋亡模型无影响,7.5mg/L浓度对共培养凋亡模型有促进作用；Bor在125umol/L浓度对HaCaT细胞的增殖无明显影响,Bor对共培养凋亡模型有逆转作用并与Bor浓度呈剂量依赖性；Men在9.38mg/L浓度对HaCaT细胞的增殖无明显影响,Men对共培养凋亡模型有逆转作用并与Men浓度呈剂量依赖性。
结论：
1.慢性湿疹患者淋巴细胞与HaCaT细胞共培养,淋巴细胞均可诱导HaCaT细胞凋亡,接种比例Lymphocyte:HaCaT=3:1为最佳共培养凋亡模型。
2.Mat在12.5ug/mL浓度对共培养凋亡模型有促进作用,50ug/mL浓度逆转作用明显。Oxy在12.5ug/mL浓度对共培养凋亡模型有促进作用,25ug/mL.50ug/mL浓度均有逆转作用。Oxy对共培养凋亡模型的促进、逆转作用均较Mat强。
3.Ber在1.5mg/L浓度对HaCaT细胞的增殖无明显影响,Ber对共培养凋亡模型有逆转作用并与Ber浓度呈剂量依赖性。
4.Pae在15.625mg/L勺浓度对HaCaT细胞的增殖无明显影响,75mg/L.15mg/L对共培养凋亡模型无影响,7.5mg/L浓度对共培养凋亡模型有促进作用。
5.Bor在125umol/L浓度对HaCaT细胞的增殖无明显影响,Bor对共培养凋亡模型有逆转作用并与Bor浓度呈剂量依赖性。
6.Men在9.38mg/L浓度对HaCaT细胞的增殖无明显影响,Men对共培养凋亡模型有逆转作用并与Men浓度呈剂量依赖性。
Background and Objective:
Chronic eczema is a common clinical disease, frequently-occurring disease, its etiology is complex, a variety of skin lesions, recurrent, severe itching, the treatment of difficult, seriously affect the patient's physical and mental health. The results confirmed that the cytokine induced by chronic eczema patients with the differentiation of Thl∨Th2cells imbalance secretion affects the development of eczema, the skin lesions of patients with chronic eczema hints of infiltration of T lymphocytes play an important role in the pathogenesis of chronic eczema, forming cell keratinocytes induced activation of T cells apoptosis as the turning point of eczema chronicity, blocking occurs horny the formation of cell apoptosis can reduce chronic eczema process. HaCaT cell lines maintained normal human keratinocyte differentiation and proliferation characteristics of cells, formation of cell physiological function of horny generic model, exploring the use of lymphocytes and HaCaT cells were cultured in vitro cell model to establish chronic eczema, efficacy for chronic eczema experiment and drug screening test has important significance.
Eczema nano cream for Professor Yang Zhibo based on years of clinical experience in the treatment of chronic eczema, external preparation of the combination of nanotechnology, preclinical studies demonstrate efficacy. This study intends to explore the lymphocytes and HaCaT cells, apoptosis of eczema nano cream composition on chronic eczema in vitro cell model of HaCaT cells was established and eczema, mechanism of nano cream constituents of chronic eczema cell model in vitro for the treatment of chronic eczema, provide the basis for establishing the in vitro cell model of chronic eczema and the clinical medication.
Method:
Through the orthogonal design experiments to explore the best model to establish apoptosis of lymphocytes and HaCaT cells co-culture of pathogenesis of chronic eczema, simulation of cells in vitro, concentrations of MTT are used to study the eczema nano cream main component of the cell viability of HaCaT, drug intervention in cells was detected by flow cytometry and TUNEL co-culture apoptosis rate of HaCaT cells model.
Results:
Chronic lymphocyte in patients with eczema and HaCaT cells to1:1,1:3,3:1ratio were inoculated separately co-culture of24h,48h,72h, lymphocytes could induce apoptosis of HaCaT cells; Lymphocytes and HaCaT cells in a co-culture of72h, inoculation ratio of Lymphocyte:HaCaT=3:1is the best co-culture apoptosis model; Mat in50ug/mL and concentration on the growth of HaCaT cells had no obvious inhibitory effect, the concentration of12.5ug/mL can promote the apoptosis of co-cultured model, the concentration of25ug/mL reversal effect is not apparent, the concentration of50ug/mL reversal effect. Oxy in50ug/mL and HaCaT cell activity had no effect on the concentration,12.5ug/mL concentration had a stimulating effect on co-culture apoptosis model,25ug/mL,50ug/mL levels were reversal effect. Promote, reversal effect of Oxy on apoptosis in the co-culture model was more strong than Mat; Ber in the concentration of1.5mg/L on the proliferation of HaCaT cells had no obvious effect, Ber has the reverse effect on the co-culture apoptosis model was dose dependent and Ber concentration; No significant influence of concentration of Pae in the15.625mg/L proliferation of HaCaT cells,75mg/L,15mg/L had no effect on the apoptosis of co-cultured model,7.5mg/L concentration had a stimulating effect on co-culture apoptosis model; Bor in the concentration of125umol/L on the proliferation of HaCaT cells had no obvious effect, Bor has the reverse effect on the co-culture apoptosis model was dose dependent and Bor concentration; Men in the concentration of9.38mg/L on the proliferation of HaCaT cells had no obvious effect, Men has the reverse effect on the co-culture apoptosis model was dose dependent and Men concentration.
Conclusion:
1.Eczema patients with chronic lymphocytes co-cultured with HaCaT cells, lymphocytes can induce the apoptosis of HaCaT cells, Lymphocyte:HaCaT=3:1is the best inoculation proportion co-culture apoptosis model.
2.Mat can promote the apoptosis of co-culture model in12.5ug/mL concentration,50ug/mL concentration reversal effect. Oxy can promote the apoptosis of co-cultured model when the concentration of12.5ug/mL,25ug/mL,50ug/mL levels were reversal effect. Promote, reversal effect of Oxy on apoptosis in the co-culture model was more strong than Mat.
3.Ber in the concentration of1.5mg/L on the proliferation of HaCaT cells had no obvious effect, Ber has the reverse effect on the co-culture apoptosis model was dose dependent and Ber concentration.
4.Pae in the concentration of15.625mg/L on the proliferation of HaCaT cells had no obvious effect,75mg/L,15mg/L had no effect on apoptosis model of co-culture,7.5mg/L concentration had a stimulating effect on co-culture apoptosis model.
5.Bor in the concentration of125umol/L on the proliferation of HaCaT cells had no obvious effect, Bor has the reverse effect on the co-culture apoptosis model was dose dependent and Bor concentration.
6.Men in the concentration of9.38mg/L on the proliferation of HaCaT cells had no obvious effect, Men has the reverse effect on the co-culture apoptosis model was dose dependent and Men concentration.
慢性湿疹作为临床一种常见病、多发病,其病因复杂、皮损多样、反复发作、剧烈瘙痒、治疗棘手,严重影响着患者的身心健康。多数研究结果证实慢性湿疹患者Thl/Th2细胞分化失衡引起的细胞因子分泌异常影响着湿疹的发生发展过程,慢性湿疹患者皮损研究提示T淋巴细胞浸润在慢性湿疹发病机制中有重要作用,活化的T细胞诱导角质形成细胞的凋亡为湿疹慢性化的转折点,阻断角质形成细胞的凋亡可以减轻湿疹慢性化进程的发生。HaCaT细胞株保持有正常人角质形成细胞的分化和增殖特性,为通用的研究角质形成细胞生理功能的模型,探索采用淋巴细胞和HaCaT细胞共培养来建立慢性湿疹的体外细胞模型,以进行慢性湿疹的药效学实验和药物筛选实验具有重要意义。
湿疹纳米乳膏为导师杨志波教授在多年临床经验总结的基础上,结合现代纳米技术研制的治疗慢性湿疹的外用制剂,前期临床研究证实疗效确切。本课题拟探索进行淋巴细胞和HaCaT细胞共培养的实验研究,观察湿疹纳米乳膏成分对慢性湿疹体外细胞模型HaCaT细胞的凋亡率,研究慢性湿疹体外细胞模型的建立及湿疹纳米乳膏成分治疗慢性湿疹的作用机制,为慢性湿疹体外细胞模型的建立及临床用药提供实验依据。
方法：
通过正交设计实验探索建立淋巴细胞和HaCaT细胞共培养的最佳凋亡模型,模拟慢性湿疹体外细胞的部分发病机制,采用MTT实验观察湿疹纳米乳膏主要成分对HaCaT细胞活力影响的浓度,流式细胞仪和TUNEL法检测药物干预细胞共培养模型后HaCaT细胞的凋亡率。
结果：
慢性湿疹患者淋巴细胞与HaCaT细胞以1：1、1：3、3：1的比例接种分别共培养24h、48h、72h,淋巴细胞均可诱导HaCaT细胞凋亡；淋巴细胞和HaCaT细胞以共培养72h,接种比例Lymphocyte:HaCaT=3:1为最佳共培养凋亡模型；Mat在50ug/mL及以下浓度对HaCaT细胞生长无明显抑制作用,12.5ug/mL浓度对共培养凋亡模型有促进作用,25ug/mL浓度逆转作用不明显,50ug/mL浓度逆转作用明显。Oxy在50ug/mL及以下浓度对HaCaT细胞活力无影响,12.5ug/mL浓度对共培养凋亡模型有促进作用,25ug/mL.50ug/mL浓度均有逆转作用。Oxy对共培养凋亡模型的促进、逆转作用均较Mat强；Ber在1.5mg/L浓度对HaCaT细胞的增殖无明显影响,Ber对共培养凋亡模型有逆转作用并与Ber浓度呈剂量依赖性；Pae在15.625mg/L的浓度对HaCaT细胞的增殖无明显影响,75mg/L.15mg/L对共培养凋亡模型无影响,7.5mg/L浓度对共培养凋亡模型有促进作用；Bor在125umol/L浓度对HaCaT细胞的增殖无明显影响,Bor对共培养凋亡模型有逆转作用并与Bor浓度呈剂量依赖性；Men在9.38mg/L浓度对HaCaT细胞的增殖无明显影响,Men对共培养凋亡模型有逆转作用并与Men浓度呈剂量依赖性。
结论：
1.慢性湿疹患者淋巴细胞与HaCaT细胞共培养,淋巴细胞均可诱导HaCaT细胞凋亡,接种比例Lymphocyte:HaCaT=3:1为最佳共培养凋亡模型。
2.Mat在12.5ug/mL浓度对共培养凋亡模型有促进作用,50ug/mL浓度逆转作用明显。Oxy在12.5ug/mL浓度对共培养凋亡模型有促进作用,25ug/mL.50ug/mL浓度均有逆转作用。Oxy对共培养凋亡模型的促进、逆转作用均较Mat强。
3.Ber在1.5mg/L浓度对HaCaT细胞的增殖无明显影响,Ber对共培养凋亡模型有逆转作用并与Ber浓度呈剂量依赖性。
4.Pae在15.625mg/L勺浓度对HaCaT细胞的增殖无明显影响,75mg/L.15mg/L对共培养凋亡模型无影响,7.5mg/L浓度对共培养凋亡模型有促进作用。
5.Bor在125umol/L浓度对HaCaT细胞的增殖无明显影响,Bor对共培养凋亡模型有逆转作用并与Bor浓度呈剂量依赖性。
6.Men在9.38mg/L浓度对HaCaT细胞的增殖无明显影响,Men对共培养凋亡模型有逆转作用并与Men浓度呈剂量依赖性。
Background and Objective:
Chronic eczema is a common clinical disease, frequently-occurring disease, its etiology is complex, a variety of skin lesions, recurrent, severe itching, the treatment of difficult, seriously affect the patient's physical and mental health. The results confirmed that the cytokine induced by chronic eczema patients with the differentiation of Thl∨Th2cells imbalance secretion affects the development of eczema, the skin lesions of patients with chronic eczema hints of infiltration of T lymphocytes play an important role in the pathogenesis of chronic eczema, forming cell keratinocytes induced activation of T cells apoptosis as the turning point of eczema chronicity, blocking occurs horny the formation of cell apoptosis can reduce chronic eczema process. HaCaT cell lines maintained normal human keratinocyte differentiation and proliferation characteristics of cells, formation of cell physiological function of horny generic model, exploring the use of lymphocytes and HaCaT cells were cultured in vitro cell model to establish chronic eczema, efficacy for chronic eczema experiment and drug screening test has important significance.
Eczema nano cream for Professor Yang Zhibo based on years of clinical experience in the treatment of chronic eczema, external preparation of the combination of nanotechnology, preclinical studies demonstrate efficacy. This study intends to explore the lymphocytes and HaCaT cells, apoptosis of eczema nano cream composition on chronic eczema in vitro cell model of HaCaT cells was established and eczema, mechanism of nano cream constituents of chronic eczema cell model in vitro for the treatment of chronic eczema, provide the basis for establishing the in vitro cell model of chronic eczema and the clinical medication.
Method:
Through the orthogonal design experiments to explore the best model to establish apoptosis of lymphocytes and HaCaT cells co-culture of pathogenesis of chronic eczema, simulation of cells in vitro, concentrations of MTT are used to study the eczema nano cream main component of the cell viability of HaCaT, drug intervention in cells was detected by flow cytometry and TUNEL co-culture apoptosis rate of HaCaT cells model.
Results:
Chronic lymphocyte in patients with eczema and HaCaT cells to1:1,1:3,3:1ratio were inoculated separately co-culture of24h,48h,72h, lymphocytes could induce apoptosis of HaCaT cells; Lymphocytes and HaCaT cells in a co-culture of72h, inoculation ratio of Lymphocyte:HaCaT=3:1is the best co-culture apoptosis model; Mat in50ug/mL and concentration on the growth of HaCaT cells had no obvious inhibitory effect, the concentration of12.5ug/mL can promote the apoptosis of co-cultured model, the concentration of25ug/mL reversal effect is not apparent, the concentration of50ug/mL reversal effect. Oxy in50ug/mL and HaCaT cell activity had no effect on the concentration,12.5ug/mL concentration had a stimulating effect on co-culture apoptosis model,25ug/mL,50ug/mL levels were reversal effect. Promote, reversal effect of Oxy on apoptosis in the co-culture model was more strong than Mat; Ber in the concentration of1.5mg/L on the proliferation of HaCaT cells had no obvious effect, Ber has the reverse effect on the co-culture apoptosis model was dose dependent and Ber concentration; No significant influence of concentration of Pae in the15.625mg/L proliferation of HaCaT cells,75mg/L,15mg/L had no effect on the apoptosis of co-cultured model,7.5mg/L concentration had a stimulating effect on co-culture apoptosis model; Bor in the concentration of125umol/L on the proliferation of HaCaT cells had no obvious effect, Bor has the reverse effect on the co-culture apoptosis model was dose dependent and Bor concentration; Men in the concentration of9.38mg/L on the proliferation of HaCaT cells had no obvious effect, Men has the reverse effect on the co-culture apoptosis model was dose dependent and Men concentration.
Conclusion:
1.Eczema patients with chronic lymphocytes co-cultured with HaCaT cells, lymphocytes can induce the apoptosis of HaCaT cells, Lymphocyte:HaCaT=3:1is the best inoculation proportion co-culture apoptosis model.
2.Mat can promote the apoptosis of co-culture model in12.5ug/mL concentration,50ug/mL concentration reversal effect. Oxy can promote the apoptosis of co-cultured model when the concentration of12.5ug/mL,25ug/mL,50ug/mL levels were reversal effect. Promote, reversal effect of Oxy on apoptosis in the co-culture model was more strong than Mat.
3.Ber in the concentration of1.5mg/L on the proliferation of HaCaT cells had no obvious effect, Ber has the reverse effect on the co-culture apoptosis model was dose dependent and Ber concentration.
4.Pae in the concentration of15.625mg/L on the proliferation of HaCaT cells had no obvious effect,75mg/L,15mg/L had no effect on apoptosis model of co-culture,7.5mg/L concentration had a stimulating effect on co-culture apoptosis model.
5.Bor in the concentration of125umol/L on the proliferation of HaCaT cells had no obvious effect, Bor has the reverse effect on the co-culture apoptosis model was dose dependent and Bor concentration.
6.Men in the concentration of9.38mg/L on the proliferation of HaCaT cells had no obvious effect, Men has the reverse effect on the co-culture apoptosis model was dose dependent and Men concentration.
引文
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