中兽药生物转化技术研究
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摘要
本研究以补益类中药黄芪、党参、银耳有效成分多糖与动物肠内乳酸菌、双歧菌间生物活性协同作用基础为依据,开展兽用中药的益生菌体外转化技术及应用研究,旨在为中兽药创新提供新技术和新思路。实验室从鸡肠道内容物中成功筛选到益生菌,对其驯化处理,在体外创建了益生菌转化中药有效成分多糖的技术工艺,并将其成功应用于中兽药新产品研制。
(1)从鸡肠道内容中获得了2株可在含药培养基中高效增殖的菌株,分别为乳杆菌FGM9和链球菌PILGM4。
(2)以增殖性能较好的PILGM4为发酵菌种,以产物中多糖增率为评价指标,引用人工神经网络法确定了体外发酵模型相关参数。
(3)实验模型对黄芪和党参多批次发酵试验,用湿浸法提取发酵产物中多糖。结果黄芪发酵提取物中多糖含量为70.88±10%,党参发酵产物提取物中多糖含量为82.47%。
(4)依据实验室建立的体外转化模型和发酵参数,研制成了一种含有益生菌、发酵黄芪、发酵党参的新型中兽药复方制剂。
(5)分别开展了药理、毒理、质量标准、免疫增强和促生长试验结果显示该产品属实际无毒,1%和0.5%剂量添加于饲料中有促进生长、增强鸡免疫、修复大鼠肝损伤的效果,毛蕊异黄酮葡萄苷的含量可为质量标示物。
综上所述,分离于动物肠道中的益生菌经传代驯化后可用补益类中药体外转化技术研究,补益类中药黄芪、党参经益生菌发酵后有效成分多糖提取率会有明显改善,临床前及临床试验结果表明益生菌体外转化中兽药的研究具有改善其生物学功效的意义。
Based on the bio-activity collaboration between the Astragalus, Codonopsis, Tremella polysaccharide and animal gut lactobacillus, bifidus bacteria, we disigned the experiment to find a kind of biotransforation technique and apply it to develop the traditional Chinese veterinary medicine in vitro. The aim was to provide a new technology or new idea for traditional Chinese veterinary medicine innovation. The probiotics were successfully separated from Gut of chickens. The experimental probiotics were acclimated with Ultraviolet Irradiation and the herbals. The transformation model was found for herbal polysaccharides extraction with the probiotic cultivation in vitro, and successfully applied to develop a new veterinary products.
(1) After being treated with the Astragalus, Codonopsis extracts and UV irradiation, Lactobacillus FGM9and streptococcus PILGM4were succesfully isolated from chicken gut.
(2) The parameters of fermentation model was screenned with PILGM4proliferation and the increase rate of polysaccharide in the artificial neural network.
(3) In experimental fermentation model, the results showed that the polysaccharides extraction rate in fermented product of astragalus was70.88higher than the raw Astragalus of33.53, it was82.47%higher than the raw Codonopsis of38.57%.
(4) A scale-up experimental model was found. A new product including a probiotic, the fermented Astragalus and the fermented Codonopsis was produced.
(5) The products were not any toxic and abnormal effects. It showed a helpful performance for body-weight gain, benefit for immunology, repairment for liver injury. The product quality could to be evaluated with the content of Calycosin-7-glucoside.
(1)从鸡肠道内容中获得了2株可在含药培养基中高效增殖的菌株,分别为乳杆菌FGM9和链球菌PILGM4。
(2)以增殖性能较好的PILGM4为发酵菌种,以产物中多糖增率为评价指标,引用人工神经网络法确定了体外发酵模型相关参数。
(3)实验模型对黄芪和党参多批次发酵试验,用湿浸法提取发酵产物中多糖。结果黄芪发酵提取物中多糖含量为70.88±10%,党参发酵产物提取物中多糖含量为82.47%。
(4)依据实验室建立的体外转化模型和发酵参数,研制成了一种含有益生菌、发酵黄芪、发酵党参的新型中兽药复方制剂。
(5)分别开展了药理、毒理、质量标准、免疫增强和促生长试验结果显示该产品属实际无毒,1%和0.5%剂量添加于饲料中有促进生长、增强鸡免疫、修复大鼠肝损伤的效果,毛蕊异黄酮葡萄苷的含量可为质量标示物。
综上所述,分离于动物肠道中的益生菌经传代驯化后可用补益类中药体外转化技术研究,补益类中药黄芪、党参经益生菌发酵后有效成分多糖提取率会有明显改善,临床前及临床试验结果表明益生菌体外转化中兽药的研究具有改善其生物学功效的意义。
Based on the bio-activity collaboration between the Astragalus, Codonopsis, Tremella polysaccharide and animal gut lactobacillus, bifidus bacteria, we disigned the experiment to find a kind of biotransforation technique and apply it to develop the traditional Chinese veterinary medicine in vitro. The aim was to provide a new technology or new idea for traditional Chinese veterinary medicine innovation. The probiotics were successfully separated from Gut of chickens. The experimental probiotics were acclimated with Ultraviolet Irradiation and the herbals. The transformation model was found for herbal polysaccharides extraction with the probiotic cultivation in vitro, and successfully applied to develop a new veterinary products.
(1) After being treated with the Astragalus, Codonopsis extracts and UV irradiation, Lactobacillus FGM9and streptococcus PILGM4were succesfully isolated from chicken gut.
(2) The parameters of fermentation model was screenned with PILGM4proliferation and the increase rate of polysaccharide in the artificial neural network.
(3) In experimental fermentation model, the results showed that the polysaccharides extraction rate in fermented product of astragalus was70.88higher than the raw Astragalus of33.53, it was82.47%higher than the raw Codonopsis of38.57%.
(4) A scale-up experimental model was found. A new product including a probiotic, the fermented Astragalus and the fermented Codonopsis was produced.
(5) The products were not any toxic and abnormal effects. It showed a helpful performance for body-weight gain, benefit for immunology, repairment for liver injury. The product quality could to be evaluated with the content of Calycosin-7-glucoside.
引文
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