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小麦胚乳A、B型淀粉粒形成机理及理化特性研究
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摘要
小麦是世界上最重要的粮食作物之一,其产量和品质关乎国际粮食安全与区域局势的稳定。在我国,小麦成为仅次于水稻的第二大粮食作物。随着人民生活水平的不断提高和食品工业的迅猛发展,小麦生产对品质的要求逐年提高。但长期以来,科技工作者对小麦品质的研究都主要集中在籽粒蛋白和优质氨基酸上,往往忽略了淀粉对小麦品质及食品加工品质的作用。淀粉是小麦籽粒胚乳的主要组成成分,约占籽粒干重的65%-70%是决定小麦品质重要指标。为此,本研究选择不同淀粉含量的4个小麦品种为材料,借助于十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)、双向凝胶电泳(2-DE)、飞行时间质谱仪(MALDI-TOF-MS)、扫描电镜(SEM)、布拉本德粘度分析仪、差示扫描量热仪(DSC)、激光粒度分析仪、X-射线衍射仪(X-RD)等现代测试技术与手段对其籽粒胚乳A、B淀粉淀粉粒的形成机理和理化特性进行了系统细致的研究,旨在为小麦淀粉品质育种目标的制定提供理论依据,为培育优质专用型小麦品种增加了研究基础。本文的主要研究内容与结果如下:
     1.用常量法和微量法对4个小麦品种淀粉粒提取、分离纯化以及后续的测定分析,发现常量法得到的A型淀粉粒纯度在79.46%-85.23%之间,B型淀粉粒纯度在90.27%-96.43%之间;微量法分离的A型淀粉粒纯度在92.68%-96.70%之间,B淀粉粒纯度在96.48%-99.00%之间。A、B淀粉粒分离与纯化效果在常量中均达到显著水平,在微量中达到极显著水平,即微量法提取、分离及纯化的A、B淀粉粒纯度高于常量法的,但常量法最终得到的A、B淀粉粒产物远大于微量法的。
     2.通过用扫描电镜SEM对小麦花后籽粒胚乳淀粉粒发育的持续观察发现,小麦淀粉体和A型淀粉粒出现于花后3d左右,B型淀粉粒出现于花后15d左右,淀粉体从9d开始逐渐消失,而A、B淀粉粒一直生长发育到籽粒成熟。运用聚丙烯酰胺凝胶电泳(SDS-PAGE)、双向凝胶电泳(2-DE)、飞行时间质谱(MALDI-TOF-MS)分析仪器与鉴定手段对小麦籽粒胚乳A、B型淀粉粒结合蛋白进行鉴定分析,结果表明A型淀粉粒形成的原因是它具有3种结合蛋白SGP-145, SGP-150和SGP-152,它们三者与支链淀粉,尤其与支链淀粉分支链聚合度>40(DP>40)的长分支链的支链淀粉合成有关,从而最终影响到了A型淀粉粒的形成,而B型淀粉粒因缺乏这3种结合蛋白而不能使其粒径发育到大于10μm。
     3.用扫描电镜(SEM)对小麦发育籽粒自然断面和分离纯化后的A、B型淀粉粒观察发现,小麦胚乳淀粉粒大小可以10μm为界限划分为A型和B型淀粉粒;形态上,淀粉粒可分为规则形状和反常形状。分离纯化后的小麦A型淀粉粒形态表现为蚌壳状;B型淀粉粒大多数表现为球状或椭球状。用直链、支链淀粉试剂盒(Megazyme International Ireland 2006)对发育小麦籽粒胚乳淀粉粒中直、支链和总淀粉测定,结果表明小麦籽粒胚乳中直链淀粉形成早于支链淀粉,但最终积累量支链淀粉大于直链淀粉,且A、B型淀粉粒中支链淀粉含量与小麦总淀粉含量成正相关趋势。
     4.对4小麦品种小麦籽粒胚乳A、B型淀粉粒膨胀势测定发现,B型淀粉粒膨胀势均大于各自A型淀粉粒的。用布拉本德粘度分析仪和差示扫描量热仪(DSC)对小麦籽粒胚乳A、B型淀粉粒粘度糊化和热力学特性测定结果显示,小麦A、B型淀粉粒糊化后均为天蓝色,淀粉峰值粘度和热力学糊化焓变表现为B型淀粉粒>A型淀粉粒。
     5.通过用激光粒度分析仪、X-射线衍射仪(XRD)分别对小麦籽粒胚乳淀粉粒的粒度分布和A、B型淀粉粒晶体特性的测定分析。结果显示,小麦籽粒胚乳淀粉粒的体积和表面积分布都是以10μm为界限呈双峰分布,数目分布呈单峰。小麦A、B型淀粉粒晶体结构均呈现典型的“A型”特征。A型粉粒相对结晶度均显著高于B型淀粉粒的,4个尖峰强度也呈现A型淀粉粒>B型淀粉粒。A型淀粉粒的相对结晶度与其体积分布呈极显著正相关,与B型淀粉粒的表面积和数量分布依次呈显著和极显著负相关;B型淀粉粒的相对结晶度分别与其表面积和数量分布依次呈显著和极显著正相关,与A型淀粉粒的体积分布呈极显著负相关。
Wheat is one of most important food crops in the world, yield and quality of which are suggested to have a role in international food supplies security and regional condition tranquilization. In China, wheat is the second large food crop just less than rice. With ever increasing standard of living of pepole and development of food industry, the quality requirement level for production of wheat is more and more strict. However, more scholars mainly concentrated on the research of wheat protein and amino acid in past a long period, and finally ignored the study of wheat starch. Starch accounted for 65%-70% weight in dry wheat grain, thus it was the main composition of wheat grain and was a more important quality character. Therefor, four wheat cultivars with different starch content were chosen for this research work and a series of modem detecting techniques were used to systemicly study the formation mechanism and physico-chemical characteristics of A, B type starch granules in wheat endosperm. These techniques included Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE), Two-dimensional Gel Electrophoresis (2-DE), and Matrix-assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF-MS) for the research of formation mechanism, whereas Scanning Electronic Microscope (SEM), Viscosity Amylograph (Brabender, Germany), Differential Scanning Calorimeter (DSC), Laser Particle Analyzer and X-ray Diffractometer (X-RD) etc for the research of physico-chemical characteristics to A, B type starch granules in wheat endosperm respectively. The major contents and results of this paper were as follows:
     1. Starch granule were isolated and purified from four wheat cultivars grain using macromethod and micromethod, and were tested by SEM. The results showed that the purity of A type starch granules ranged from 79.46% to 85.23%, which of B type starch granules ranged from 90.27% to 96.43% by using macromethod; if using micromethod, the purity of A type starch granules ranged from 92.68% to 96.70%, which of B type starch granules ranged from 96.48% to 99.00%.The effects of isolation and purification for A, B-type starch granule indicated significant level in macromethod, and distinct significant level in micromethod. In other words, the purity of A, B type starch granules with micromethod was higher than that of macromethod, but the ratio of A, B type starch granules with micromethod was more less than that of macromethod.
     2. The developing condition of starch granules in wheat endosperm were continuously observed with Scanning Electronic Microscope (SEM), and found that amyloplast and A type starch granules emerged at approximately 3 day post anthesis (DPA) and B type starch granules emerged at approximately 15 DPA. The amyloplast began to disappear from 9 DPA, but A and B type starch granules continuously grew till.wheat grain matured. Starch granule band protein (SGP) of wheat A, B type starch granules were separated and identified by Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE), Two-dimensional Gel Electrophoresis (2-DE), and Matrix-assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF-MS). The results showed that the reason why A type starch granules formation was that it had 3 sort of SGPs (SGP-145, SGP-150 and SGP-152), which could promote the biosynthesis of amylopectin, especially the degree of polymerization of chain length of amylopectin> 40(DP>40), and finally caused the formation of A type starch granules. The diameter of B type starch granules was less than 10μm, due to they lacked of such 3 SGPs.
     3. The natural ruptured side of kernel of different deveoping periods and the separated & purified A, B type starch granules of wheat endosperm in wheat were observed by Scanning Electron Micrograph (SEM). The results showed that wheat starch granules could be divided into A and B type by its diameter of 10μm, and could be divided into normal and abnormal by its shape. The shape of separated & purified A type starch granules was shell structure but the shape of most B type starch granules indicated spherical structure. The contents of amylose and amylopectin in A, B type starch granules of wheat endosperm of different developing periods were determined by AMYLOSE/AMYLOPECTIN ASSAY PROCEDURE, and the results indicated that the formation of amylose in starch granule of wheat endosperm was earlier than that of amylopectin, while the final content of amylopectin was much higher than that of amylose, and the amylopectin contents in A, B type starch granules had a positive correlation trend to total starch content.
     4. The swellings power of A, B type starch granules in matured wheat endosperm were measured according to method of McCormick, and the results showed that the swelling power of B-type starch granule was higher than that of A-type starch granule. Viscosity and thermal characteristics of A, B type starch granules of wheat were determined by Viscosity Amylograph (Brabender, Germany) and Differential Scanning Calorimeter (DSC). The results showed that the color of A, B type starch granules after cataplasm were sky blue, both the peak value of paste (PV) and the value of enthalpy change (△H) of B-type starch granule were higher than that of A-type starch granule.
     5. The size distribution of starch granules were determined using Laser Particle Analyzer and the crystalline properties of A, B type starch granules were determined by X-ray Diffractometer (X-RD) The results of size distribution showed that the volume and surface area in wheat endosperm displayed a bimodal distribution by its diameter of 10μm, while the number displayed a single peak. The results of crystalline properties showed that the crystalline structure of A, B type starch granules were typical "A" type and the crystalline value of A type starch granule is totally notable larger than that of B type, the same trends as the value of four shape peaks.The analysis of correlation relationship study between size distribution and crystalline properties showed that the crystallinity of A type starch granules have extremely positive significant associated with the volume of A type, but have negative or extremely negative significant with surface area and number distribution of B type starch granule, thus the crystallinity of B type starch granules have extremely negative significant with the volume of A type, whereas have positive significant or extremely significant with surface area and number distribution of B type starch granule in wheat.
引文
[1]包劲松,夏英武.水稻淀粉合成的分子生物学研究进展[J].植物学通报,1999,16(4):352-358.
    [2]蔡瑞国,尹燕枰,赵发茂,张敏,张体彬,梁太波,顾锋,戴忠民,王振林.强筋小麦胚乳淀粉粒度分布特征及其对弱光的响应[J].中国农业科学,2008,41(5):1308-1316.
    [3]曹连莆,李卫华,张薇,卢静,银永安.小麦品质生理及遗传研究与应用[M].北京:经济管理出版社,2010,1-3.
    [4]曹晓艳,冯建荣,王大江,白茹,刘月霞.2D-DIGE技术研究自交不亲和杏品种‘新世纪’花柱表达蛋白[J].中国农业科学,2011,44(4):789-797.
    [5]陈福泉,张本山,卢海凤,赵永青,张向阳.x射线衍射在淀粉颗粒结晶度研究中的应用[J].食品科学,2010,31(3):284-287.
    [6]陈义芳,张静,周卫东,张彪,马雷,韦存虚.不同品质类型小麦籽粒结构的观察比较[J].电子显微学报,2006,25(3):271-274.
    [7]戴忠民,王振林,张敏,李文阳,闫素辉,蔡瑞国,尹燕枰.不同品质类型小麦籽粒淀粉粒度的分布特征[J].作物学报,2008,34(3):465-470
    [8]戴忠民,尹燕枰,王振林.鲁麦21和济南17胚乳发育过程中淀粉粒的动态变化[J].中国农业科学,2009,42(3):816-823.
    [9]皇甫海燕,官春云.甘蓝型油菜抗菌核病近等基因系和感病亲本蛋白差异的初步研究[J].中国农业科学,2010,43(10):2000-2007.
    [10]李建生.玉米淀粉品质遗传改良研究进展[J].作物杂志,1998,(增刊):114-118.
    [11]李强,张卫东,田纪春.小麦抗白粉病基因Pm21抗病差异的蛋白质组学研究[J].中国农业科学,2009,42(8):2778-2783.
    [12]梁丽松,徐娟,王贵禧,马惠铃.板栗淀粉糊化特性与淀粉粒粒径及直链淀粉含量的关系[J].中国农业科学,2009,42(1):251-260.
    [13]刘广田,李保云,小麦的营养品质及品质改良[J].小麦研究,1997,18(1):1-5.
    [14]刘怀华,王莉雯,刘楠,刘旭,马侠,宁丽华,张华,崔德周,姜川,陈化榜.玉米花粉与花丝早期互作的蛋白质组学分析[J].中国农业科学,2010,43(24):5000-5008.
    [15]刘鹏,往静,刘钟栋,高新楼,王松.糯质小麦淀粉理化特性研究[J].粮油加工,2006,(11):72-74.
    [16]马成,徐世昌,徐琴,张朝晖,潘映红.抗条锈病小麦品系Taichuang29*6/Yr5接种条锈菌CY32后的蛋白质组学分析[J].中国农业科学,2009,42(5):1616-1623.
    [17]潘庆民,于振文,王月福.追氮时期对小麦旗叶中蔗糖合成与籽粒中蔗糖降解的影响[J].中国农业科学,2002,35(7):771-776.
    [18]彭佶松,郑志仁,刘涤.淀粉的生物合成及其关键酶[J].植物生理学通讯,1997,33(4):297-303.
    [19]舒庆尧,吴殿星,夏英武.稻米淀粉RVA谱特征与食用品质的关系[J].中国农业科学,1998,31(3):25-29.
    [20]唐如春,杨宇衡,范三红,郭蔼光.圆锥小麦ramosa2的克隆及其重组蛋白的DNA结合特性分析[J].中国农业科学,2011,44(3):439-446.
    [21]王晨阳,何英,方保停,王书丽,付雪丽,郭天财.小麦籽粒淀粉合成、淀粉特性及其调控研究进展[J].麦类作物学报,2005,25(1):109-114.
    [22]韦存虚,张军,周卫东,陈义芳,许如根.大麦胚乳小淀粉粒的发育.作物学报,2008,34(10):1788-1796.
    [23]韦存虚,张翔宇,张军,徐斌,周卫东,许如根.不同类型小麦品种大、小淀粉粒的分离特性[J].麦类作物学报,2007,27(2):255-260.
    [24]吴金芝,黄明,李友军,付国占,陈明灿,黄海霞.不同水分和氮素形态对弱筋小麦豫麦50籽粒淀粉产量和淀粉糊化特性的影响[J].中国农业科学,2009,42(5):1833-1840
    [25]夏其昌,曾嵘.蛋白质化学与蛋白质组学[M].北京:科学出版社,2004,277-278.
    [26]熊福生,高珠,詹勇昌.植物叶片中蔗糖、淀粉积累与其降解酶活性关系研究[J].作物学报,1994,20(1):52-58.
    [27]徐军望,李旭刚,朱祯.基因工程改良淀粉品质[J].生物技术通报,2000,16(1):11-19.
    [28]许培磊,白吉刚,王秀娟,宗成顺,田明.低温对不同基因型黄瓜叶片蛋白质组的影响[J].中国农业科学,2009,42(2):588-596.
    [29]闰洁,陈守才,夏志辉.橡胶树死皮病胶乳C-乳清差异表达蛋白质的筛选与鉴定[J].中国生物工程杂志,2008,28(6):28-36.
    [30]阎俊,张勇,何中虎.小麦品种糊化特性研究[J].中国农业科学,2001,34(1):1-4.
    [31]阎隆飞,李启明.基础生物化学[M].北京:农业出版社,1985,21-22.
    [32]杨学举,屈平,张彩英,刘广田.小麦A型淀粉粒大小对淀粉特性及面包品质的影响[J].河北农业大学学报,2004,27(5):01-05.
    [33]杨于兴,漆睿.x射线衍射分析(第一版)[M].上海:上海交通大学出版社,1994,75-78.
    [34]姚大年,刘广田.淀粉理化特性、遗传规律及小麦淀粉与品质的关系.粮食与饲料工业,1997,(2):36-38.
    [35]姚大年,徐风,马传喜,刘广田.中国首批面包小麦品质的研究[J].中国粮油学报,1995,10(4):1-4.
    [36]张海艳,董树亭,高荣岐.植物淀粉研究进展[J].中国粮油学报,2006,21(1):41-46.
    [37]张艳霞,丁艳锋,李刚华,王强盛,黄丕生,王绍华.直链淀粉含量不同的稻米淀粉结构、糊化特性研究[J].作物学报,2007,33(7):1201-1205.
    [38]张瑛,吴先山,吴敬德.稻谷储藏过程中理化特性变化的研究[J].中国粮油学报, 2003,18(6):20-25.
    [39]张勇,何中虎.我国春播小麦淀粉糊化特性研究[J].中国农业科学,2002,35(5):471-475.
    [40]钟秋平,谢碧霞,王森,李安平,李清平,钟文斌,邓文清,邓小清.超微粉碎对橡实淀粉颗粒晶体特性影响的研究[J].江西农业大学学报,2007,29(4):594-597.
    [41]朱帆,徐广文,丁文平.小麦淀粉颗粒大小和糊化特性的相关性研究[J].中国粮油学报,2006,21(4):32-34.
    [42]Aboubacar A, Moldenhanuer K A K, McClung A M, Beighlcy D H, Hamaker B R.Effect of growth location in the united states on amylase content, amylopectin fine structure, and thermal properties of starches of long grain rice cultivars [J]. Cereal Chemistry, 2006,83(1):93-98.
    [43]Ahmadi A and Baker D A. The effect of water stress on the activities of key regulatory enzymes of the sucrcse to starch pathway in wheat [J]. Plant Growth Regul,2001,35(1): 81-91.
    [44]Ainsworth C, Clark J, Balsdon J. Expression, organization and structure of the genes encoding the waxy protein(granule-bound starch synthase)in wheat [J]. Plant Molecular Biology,1993,22(1):67-82.
    [45]Akashi H, Takahashi M, Endo S. Evaluation of starch properties of wheats used forChinese yellow-alkaline noodles in Japan [J].Cereal Chemistry,1999,76(1):50-55.
    [46]Anderson N L, Matheson A D, Steiner S. Proteomics:applications in basic and applied biology [J]. Current Opinion in Biology,2000,11(4):408-412.
    [47]Araki E, Miura H, Sawada S. Differential effects of the null alleles at the three Wx loci on the starch-pasting properties of wheat [J]. Thcor Appl Genet,2000,100(7):1113-1120.
    [48]Atichokudomchai N, Varavinit S, Chinachofi P. Gelatinization transitions of acid-modified tapioca starches by differential scanning calorimetry (DSC) [J]. Starch/Starke,200254 (7):296-302.
    [49]Baga M, Nair R B, Repellin A, Scoles G J, Chibbar R N. Isolation of a cDNA encoding a granule-bound 152-kilodalton starch-branching enzyme in wheat [J]. Plant Physiology, 2000,124(1):253-26.
    [50]Balk B K, Czuchajowska Z, Pomeranz Y. An SDS-FY test to evaluate quality of wheat for oriental noodles [J]. Journal of Cereal Science,1994,20(2):191-201.
    [51]Ball S, Guan H P, James M, Myers A, Keeling P, Mouille G, Buleon A, Colonna P, Preiss J. From glycogen to amylopectin:a model for the biogenesis of the plant starch granule [J]. Cell,1996,86(3):349-352.
    [52]Ball S G and Morell M K. From bacterial glycogen to starch:understanding the biogenesis of the plant starch granule [J]. Annual Review of Plant Biology,2003,54: 207-233.
    [53]Ball S G, Vande W M, Visser R G F. Progress in understanding the biosynthesis of amylose [J]. Trends Plant Science,1998,3(12):462-467.
    [54]Batey I L, Curtin B M, Moore S A. Optimization of Rapid-Visco-Analyse test conditions for predicting Asian noodle quality [J].Cereal Chemistry,1997,74(4):497-501.
    [55]Bechtel D, Zayas I, Kaleikan A and Pomeran Z. Size-distribution of wheat starch granules during endosperm development [J]. Cereal Chemistry,1990,67(1):59-63.
    [56]Bechtel D, Zayas I, Dempster R, Wilson J D. Size-distribution of starch granules isolated from hard red winter and soft red winter wheat [J]. Cereal Chemistry,1993,70(1): 238-240.
    [57]BelloPerez L A, ParedesLopez O, Roger P, Colonna P. Amylopectin:properties and fine structure [J]. Food Chemistry,1996,56(2):171-176.
    [58]Bertoft E. On the nature of categories of chains in amylopectin and their connection to the super helix model [J]. Carbohydrate Polymers,2004,57(2):211-224.
    [59]Bescher C, Gerke V. Annexins-unique membrane binding protein swith diverse functions [J]. Cell Science,2004,117(13):2631-2639.
    [60]Bhattacharya M, Corke H. Selection of desirable starch pasting properties in wheat for use in white salted or yellow alkaline noodles [J]. Creal Chemistry,1996, 73(6):721-728.
    [61]Blankenhorn D, Phillips J, Slonczewski J L. Acid-and base-induced proteins during aerobic and anaerobic growth of Escherichia coli revealed by two-dimensional gel electrophoresis [J]. Journal of bacteriology,1999,181(7):2209-2216.
    [62]Briarty L G, Hughes C E, Evers A D. The developing endosperm of wheat a stereological analysis [J]. Annals of Botany,1979,44(6):641-658.
    [63]Buleon A., Bizot H., Delage M. M. and Pontoire B. Comparison of X-ray diffraction patterns and sorption properties of the hydrolyzed starches of potato, wrinkled pea, smooth pea, broad bean and wheat [J]. Carbohydrate Polymers,1987,7(6):461-462.
    [64]Burton M K, Bewley J D, Smith A M. Starch branching enzymes belonging to distinct enzyme families are differentially expressed during pea embryo development [J]. Plant Journal,1995,7(1):3-15
    [65]Buttrose M S. Ultrastructure of the developing wheat endosperm [J]. Australian Journal of Biological Sciences,1963,16(2):305-310.
    [66]Chao S, Sharp P J, Worland A J, Warham E J, Koebner R M D, Gale M D. RFLP-based genetic maps of wheat homoeologous group 7 chromosomes [J]. Theor Appl Genet, 1989,78(4):495-504.
    [67]Cheetham N W H, Tao L. Variation in crystalline type with amylose content in maize starch granules:an X-ray powder diffraction study [J]. Carbohydrate Polymers,1998, 36(4):277-284.
    [68]Chiotelli E, Meste M L. Effect of small and large wheat starch granules on thermo-mechanical behavior of starch [J]. Cereal Chemistry,2002,79(6):286-293.
    [69]Considine D M. Foods and Food Production Encyclopedia(ed) [M]. New York:John Wiley Inc,1982.55-56.
    [70]Cooke D, Gidley M J. Loss of crystalline and molecular order during starch gelatinization:origin of the enthalpic transition [J]. Carbohydrate research,1992, 227(6):103-112.
    [71]Crosbie G B. The relationship between starch swelling properties, paste viscosity and boiled noodle quality in wheat flour [J]. Journal of Cereal Science,1991,13(2): 145-150.
    [72]Chiotelli E, Meste M L. Effect of small and large wheat starch granules on thermomechanical behavior of starch [J]. Cereal Chemistry,2002,79 (2):286-293.
    [73]Cutler P, Birrell H, Haran M. Proteomics in pharmaceutical research and development [J]. Biochemical Society Transactions,1999,27(4):449-458.
    [74]Deatherage W L, MacMasters M M, Rist C E. A partial survey of amylose content in starch from domestic and foreign varieties of corn, wheat, and sorghum and from some other starch bearing plants [J]. Transactions of American Association of Cereal Chemistry,1955 13(1):31-33.
    [75]Dengate H. and Meredith P. Variation in size distribution of starch granules from wheat grin [J]. Journal of Cereal Science,1984,2(2):83-90.
    [76]Denyer K, Dunlap F, Thorbjornsen T, Keeling P, Smith A M. The major form of ADP glucose pyrophosphorylase in maize endosperm is extraplastidial [J]. Plant physiology, 1996,112(2):779-783.
    [77]Doane W M. Opportunities and challenges for new industrial nses of starch [J]. Cereal Food World,1994,39(1):556-562.
    [78]Donald B, Bechtel D, Jeff D M, Wilson T. Amyloplast Formation and Starch Granule Development in Hard Red Winter Wheat [J]. Cereal Chemistry,2003,80(2):175-183.
    [79]Edwards A, Fulton D C, Hylton C M, Jobling S A, Gidley M, Ressner U, Martin C and Smith A M. A combined reduction in activity of starch synthases Ⅱ and Ⅲ of potato has novel effects on the starch of tubers [J]. The Plant Journal,1999,17(3):251-261.
    [80]Ellia R P, Cochrane M P, Dale M F B, Duffus C M, Lynn A, Morrison I M, Prentice R D M, Swauston J S and Tiller S A. Starch production and industrial use [J]. Journal of Science and Food Agricure,1998,77(3):289-311.
    [81]Evers A D. Scanning electron microscopy of wheat starch. Ⅲ. Granule development in the endosperm [J]. Starch/Starke,1971,23(5):157-162.
    [82]Evers A D. The size distribution among starch granules in wheat endosperm [J].Starch/Starke,1973,25(9):303-304.
    [83]Evers A D and Lindley J. The particle size distribution in wheat endosperm starch [J]. Journal of Food and Agricure Science,1977,28(1):98-101.
    [84]Fannon J E, Gray J A, Clalnawan N, Huber K C, BeMiller N. Heterogeneity of starch granules and the effect of granule channelization on starch modification [J]. Cellulose, 2004,11(2):247-254.
    [85]Figeys D, McBroom L D, Moran M F. Masss Spectry for the Study of Protein-Protein Interactions [J]. Methods,2001,24(3):230-239.
    [86]Fishman M L, Cook P, White B, Damert W. Size distributions of amylose and amylopectin solubilized from corn starch granules [J]. Carbohydrate Polymers,1995, 26(4):245-253.
    [87]Florens L, Washburn M P, Raine J D, Anthony R M, Grainger M, Haynes J D, Moch J K, Muster N, Sacci J B, Tabb D L, Witney A A, Wolters D, Wu Y M, Gardner M J, Holder A A, Sinden R E, Yates J R, Carucci D J. A proteomic view of the Plasmodium falciparum life cycle [J]. Nature,2002,419(6906):520-526.
    [88]Fountoulakis M, Hardmaier R, Schuller E. Differences in protein level between neonatal and adult brain [J]. Electrophoresis,2000,21(3):673-678.
    [89]French D. Organization of starch granules. In R Whistler, J BeMiller, E Paschall, eds, Starch Chemistry and Technology, Ed 2 [M]. Academic Press, New York,1984, 184-242.
    [90]Fujita N, Hasegawa H, Taira T. The isolation and characterization of a waxy mutant of diploid wheat (Triticum monococcum L.) [J]. Plant Science,2001,160(4):595-602.
    [91]Gaines C S, Raeker M, Tilley M. Associations of starch gel hardness, granule size waxy allelic expression, thermal pasting, milling quality and kernel texture of 12 soft wheat cultivars [J]. Cereal Chemistry,2000,77(2):163-169.
    [92]Gao M, Wanat J, Stinard P S, James M G, Myers A M. Characterization of dull1, a maize gene coding for a novel starch synthase [J]. Plant cell,1998,10(3):399-412.
    [93]Gerard C, Planchot V, Colonna P, Bertoft E. Relationship between branching density and crystalline structure of A and B-type maize mutant starches [J]. Carbohydrate Research 2000,326(2):130-144.
    [94]Gomez D F, Iglesias A A. ADP-glucose pyrophosphorylase from wheat endosperm. Purification and characterization of an enzyme with novel regulatory properties [J]. Planta,2002,214(3):428-434.
    [95]Granier F. Extraction of plant proteins for two- dimensional electrophoresis [J]. Electrophoresis,1988,9(11):712-718.
    [96]Gray J A, BeMiller J N. Accessibility of starch granules to fatty acylamides [J]. Cereal Chemistry,2001, (78):236-242.
    [97]Greenet W, Hannahl C. Enhanced stability of maize endosperm ADP-glucose pyrophosphorylase is grained through mutants that alter subunit interactions [J]. Proc Natl Acad Science USA,1998,95(22):13342-13347.
    [98]Grunenfelder B, Rummel G, Vohradsky J. Proteomic analysis of the bacterial cell cycle [J]. PNAS,2001,98(8):4681-4686.
    [99]Guan H, Preiss J. Differentiation of the properties of the branching isozymes from maize (Zea mays) [J]. Plant Physiology,1993,102(4):1269-1273.
    [100]Darlington H F, Tecsi L, Harris N, Griggs D L, Cantrell I C and Shewry P R. Starch Granule Associated Proteins in Barley and Wheat [J]. Journal of Cereal Science,2000, 32(1):21-29.
    [101]Helbert W, Schuelein M, Henrissat B. Electron microscope investigation of the diffusion of Bacillus licheniformis (-amylase into com starch granules) [J]. International Journal of Biological Macromolecules,1996 19(3):165-169.
    [102]Higley J S, Love S L, Nelson J E, Huber K C. The rapid viseo analyzer (RVA) as a tool for differentiating potato cultivars on the basis of flour pasting properties. American Journal of Potato Research,2003,80 (1):195-206.
    [103]Hommais F, Winter C L, Labas V. Effect of mild acid pH on the functioning of bacterial membranes in Vibrio cholerae [J]. Proteomics,2002,2(5):571-579.
    [104]Hoover R. Composition, molecular structure, and physicochemical properties of tuber and root starches:a review [J]. Carbohyd Polym,2001,45(3):253-267.
    [105]Huber K C and BeMiller J N. Channels of maize and sorghum starch granules [J]. Carbohydr Polymers,2000,41(3):269-276.
    [106]Huber K C and BeMiller J N. Location of sites of reaction within starch granules [J].Cereal Chemistry,2001,78(2):173-180.
    [107]Hyun S K, Kerry C and Huber W. Channels within soft wheat starch A-and B-type granules [J]. Journal of Cereal Science,2007,9(2):1-14.
    [108]Ian J. Tetlow. Understanding storage starch biosynthesis in plants:a means to quality improvement [J]. NRC Canada,2006,84(1):1167-1185.
    [109]Imberty A, Chanzy H, Pdrez S. The double-helical nature of the crystalline part of A-starch [J]. Journal of Molecular Biology,1988,201(2):365-378.
    [110]James M G, Robertson D S, Myers A M. Chraracterization of the maize gene sugary 1, a determinant of starch composition in kernels [J].The Plant Cell,1995,7(4):417-429.
    [111]JaneH L, Vasanthan T, Hoover R, Rossnage B G. Starch from hull-less barley:Ⅳ. Morphological and structural changes in waxy, normal and high-amylose starch granules during heating [J]. Food Research International,2004,37(5):417-428.
    [112]Jane J L, Chen J. Effect of amylose molecular size and amylopectin branch chain length on paste properties of starch [J]. Cereal Chemistry,1992,69 (1):60-65.
    [113]Jenkins P J, Donald A M. The influence of amylose on starch granule structure [J]. International Journal of Biological Macromolecules,1995,17(6):315-321.
    [114]Jenner C F, Siwek K, Hawken J S. The synthesis of [14C] sucrose in isolated wheat grains is dependent upon the activity of soluble starch synthase [J]. Australia Journal Plant Physiology,1993,20(3):329-335.
    [115]Ji Y, Seetharaman K, Wong K, Pollak L M, Duvick S, Jane J, White P J. Thermal and structural properties of unusual starches from developmental corn lines [J]. Carbohydrate Polymers,2003 51(4):439-450.
    [116]Jones R J, Roessler J, Ouattar S. Thermal environment during endosperm cell division in maize:effects on number of endosperm cells and starch granules [J]. Crop Science, 1985,25(5):830-834.
    [117]Jyothi AN, Sasikiran K, Sajeev M S, Revamma R, Moorthy S N. Gelatinisation properties of cassava starch in the presence of salts, acid and oxidizing agents [J]. Starch/Starke,2005,57(11):547-555.
    [118]Karathanos V T and Saravacos G D. Porosity and size distribution of starch materials [J]. Journal of Food Engineering,1993,18(3):259-280.
    [119]Keeling P L, Bacon P J, Holt D C. Elevated temperature reduces starch deposition in wheat endosperm by reducing the activity of soluble starch synthase [J]. Planta,1993, 191(3):342-348.
    [120]Khalil N F, Duncan H J. The sillca content of plant polysaccharides [J]. Journal of the Science of Food and Agriculture,1981,32(4):415-418.
    [121]Kim S Y, Wiesenbom D P, Orr P H, Grant L A. Screening potato starch for novel properties using differential scanning calorimetry [J]. Journal of Food Science,1995, 60(5):1060-1065.
    [122]Kim W, Johnson J W, Graybosch R A, Gaines C S. Physicochemical properties and end-uses quality of wheat starch as a function of waxy protein alleles [J]. Journal of Cereal Science,2003,37(4):195-204.
    [123]Kleczkowski L A, Villand P, Luthi E. Insensitivity of barley endosperm ADP-glucosepyrophosphorylase to 3-phosphoglycerate and orthophosphate regulation [J]. Plant Physiology,1993,101(1):179-185.
    [124]Komiya T and Nara S. Changes in crystallinity and gelatimzafion phenomena of potato starch by acid treatment [J]. Starch/Starke,1986 38(2):9-13.
    [125]Ko Y T, Dong Y L, Hsieh Y F, Kuo J C. Morphology, Associated Protein Analysis, and Identification of 58-kDa Starch Synthase in Mungbean (Vigna radiataL. cv. KPS1) Starch Granule Preparations [J]. Agriculture and Food Chemistry,2009,57(10): 4426-4432.
    [126]Krueger B R, Knutson C A, Inglett G E and Walker C E. A differential scanning calorimetry study on the effect of annealing on gelatinization behaviour of corn starch [J]. Journal of Food Science,1987,52(3):715-718.
    [127]Legrain P, Selig L. Genome-wide protein interaction maps using two-hybrid systems [J]. FEBS,2000,480(1):32-36.
    [128]Li J, Corke H. Playsicochemical properties of maize starches expressing dull and sugary-2 mutants in different genetic bachground [J]. Journal of Agriculture and Food Chemistry,1999,47(1):4939-4943.
    [129]Li J Y, Yeh A I. Relationships between thermal, rheological characteristics and swelling power for various starches [J]. Jounal of Food Engineering,2001 50(3):141-148.
    [130]Litwin C M, Calserwood S B. Analysis of the complexity of gene regulation by Fur in Vibrio cholerae [J]. Journal of bacteriology,1994,34(1):240-248.
    [131]Lin J H, Chang Y H. Molecular degradation rate of rice and com starches during acid-methanol treatment and its relation to the molecular structure of starch [J]. Journal of Agriculture and Food Chemistry,2006,54(16):5880-5886.
    [132]Madsen M H and Christensen D H. Changes in viscosity properties of potato starch during growth [J]. Starch/starke,1996,48(8):245-249.
    [133]Mangalika W H A, Miura H, Yamauchi H, Noda T. Properties of starches from near isogenic wheat lines with different wx protein deficiencies [J].Cereal Chemistry,2003 80(6):662-666.
    [134]Marana C, Garcia O F, Carbonero P. Differential expression of two types of sucrose synthase-encoding genes in wheat in response to anaerobiosis cold shock and light [J]. Gene,1990,88(2):167-172.
    [135]Martin C, Smith A M. Starch Biosynthesis [J]. Plant Cell,1995,7(7):971-985.
    [136]MatHashim D. B and Moorthy S N. The effect of low levels of antioxidants on the swelling and solubility of cassava starch [J]. Starch/Starke,1992,44(12):471-475.
    [137]Matveev Y I, Van Soest J J G, Nieman C, Wasserman L A, Protserov V A, Ezemitskaja M, Yuryev V P, The relationship between the rmodynamic and structural properties of low and high amylose maize starches [J]. Carbohydrate Polymers,2001,44(2): 151-160.
    [138]McCormick K M, Panozzo J F, Hong S H. A swelling power test for selecting potential noodle quality wheats [J]. Australian Journal of Agricultural Research,1991,42(3): 317-323.
    [139]Meredith P, Christchurch K. Large and Small Starch Granules in Wheat-Are They Really Different [J]. Starch/Stiirke,1981,2(4):40-44.
    [140]Miho H, Chihako K O, Masaharu S. Ghosts of B-type Wheat Starch Granules in Concentrated KI/I2 Solution [J]. Starch Journal,2004,57(8):384-387.
    [141]Ming G, Ravindra N, Chibbar B. Isolation, characterization, and expression analysis of starch synthase Ila cDNA from wheat (Triticum aestivum L.) [J]. Genome,2000,43(4): 768-775.
    [142]Ming P, Gao M, Eits M, Abdel P. Huc R N, Chibbar N. Separation and Characterization of A-and B-Type Starch Granules in Wheat Endosperm [J]. Cereal Chemistry,1999, 76(3):375-379.
    [143]Miura H and Sugawara A. Dosage effects of the three Wx genes on amylose synthesis in wheat endosperm [J]. Theor Appl Genet,1996,93:1066-1070.
    [144]Monille G. Preamylopectin processing:Amandatory step for starch biosynthesis in plants [J].The Plant Cell,1996,8(8):1353-1366.
    [145]Morell M K, Blennow A, Hashemi B K, Samuel M S. Differential expression and properties of starch branching enzyme isoforms in developing wheat endosperm. Plant Physiology,1997,113(1):201-208.
    [146]Morell M K, Cathier S. The biochemistry and molecular biology of starch synthesis in cereals [J]. Australia Journal Plant Physiology,1995,22(4):647-660.
    [147]Morrison W R. Uniqueness of wheat starch. In:Wheat is Unique, Pomeranz Y fed 1 American Association of Cereal Chemists [J]. St, Paul, MN.1989,60(5):132-214.
    [148]Nakamura Y, Umemoto T, Takahata Y, Komae K, Amano E, Satoh H. Changes in structure of starch and enzyme activities affected by sugary mutations in developing rice endosperm:possible role of starch debranching enzyme(R-enzyme)in amylopectin biosynthesis [J]. Physioloy Plant,1996,97(3):491-498.
    [149]Nguyen Q D, Jensen C T B, Kristensen P G. Experimental and modelling studies of the flow properties of maize and waxy maize starch pastes [J]. Chemistry Engineering Journal,1998,70(2):165-171.
    [150]Nielsen T H, Baunsgaard L, Blennow A. Intermediary glucan structures formed during starch granule biosynthesis are enriched in short side chains, a dynamic pulse labeling approach [J]. Journal of Biological Chemistry,2002,277(23):20249-20255.
    [151]Noda T, Takahata Y, Nagata T. Properties of sweet potato starches from different tissue zones [J]. Starch/Stiirke,1992,44(10):365-368.
    [152]Okitatw K. Is there an alternative pathway for starch synthesis [J]. Plant Physiology, 1992,100(2):560-564.
    [153]Oostergetel G T and Van Bruggen E F. The crystalline domains in potato starch granules are arranged in a helical fashion [J]. Carbohydrate Polymers,1993,21(1):7-12.
    [154]Paris M, Bizot H, Emery J, Buzare A, Buleon A. Crystallinity and structuring role of water in native and recrystallized starches by 13C CP-MAS NMR spectroscopy [J]. Carbohydrate Polymers,1999,39(4):327-339.
    [155]Parker M L. The relationship between A-type and B-type starch granules in the developing endosperm of wheat [J]. Journal of Cereal Science,1985,3(4):271-278.
    [156]Parker R and Ring S G. Aspects of the physical chemistry of starch [J]. Journal of Cereal Science 2001,34(1):1-17.
    [157]Paterson J L, Hardacre A, Li P, Rao M A. Rheology and granule size distributions of corn starch dispersions from two genotypes and grown in four regions [J]. Food Hydrocolloids,2001,15(4-6):453-459.
    [158]Patron N J, Smith A M,Fahy B F, Hylton C M, Naldrett M J, Rossnagel BG, Denyer K. The altered pattern of amylose accumulation in the endosperm of low-amylose barley cultivars is attributable to a single mutant allele of granule-bound starch synthase I with a deletion in the 50-non-coding region [J]. Plant Physiology,2002,130(1):190-198.
    [159]Peng M S, Ming G, Monica B, Pierre H, and Ravindra N. Chibbar. Starch-Branching Enzymes Preferentially Associated with A-Type Starch Granules in Wheat Endosperm [J]. Plant Physiology,2000,124(1):265-272.
    [160]Perrot M, Saglicocco F, Mini T. Two-dimensional gel protein database of Saccharomyces cerevisiae [J]. Electrophoresis,1999,20(11):2280-2298.
    [161]Plomion C, Pionneau C, Brach J. Compression Wood-Responsive Proteins in Developing Xylem of Maritime Pine [J]. Plant Physiology,2000,123(3):959-970.
    [162]Preiss J and Sivak M. Starch synthesis in sinks and sources.In:Zamski E, Schaffter AA, eds.Photoassimilate distribution in plants and crops:sink-source relationships [M]. New York:Marcel Dekker,1996,63-96.
    [163]Racker M 6, Gaines C S, Fianey P L, Donelson T. Granule size distribution and chemical composition of starches from 12 soft wheat cultivars [J]. Cereal Chemistry, 1998,75(5):721-728.
    [164]Rabilloud T. Two-dimensional gel electrophoresis in proteomics:old fashioned but it still climbs up the mountains [J]. Proteomics,2002,2(1):3-10.
    [165]Raja K C M and Ramakrishna S V. Influence of salts on pasting temperature characteristic of cassava [J]. Food Chemistry,1988,28(4):311-317.
    [166]Ranam S. Genetic manipulation of starch properties in wheat [J]. Chemistry in Australia, 1994,9(3):517-518.
    [167]Rahman S, KosarH B, Samuel M S, Hill A, Abbot D C, Skerritt J H, Preiss J, Appels R, Morell M K. The major proteins of wheat endosperm starch granules [J]. Australian Journal of Plant Physiology,1995,22(5):793-803.
    [168]Richard F, Tester, John K, Xin Q. Starch composition, fine structure and architecture [J]. Journal of Cereal Science,2004,39(3):151-165.
    [169]Richardson S, Cohen A, Gorton L.High-performance nion-exchange chromatography electrospray mass spectrometry for investigation of the substituent distribution in hydroxypropylated potato amylopectin starch [J]. Journal of Chromatography A,2001, 917(1-2):111-121.
    [170]Rigven A. Heat inaction of starch systhase in wheat endosperm [J]. Plant Physiology, 1986,81(1):449-457.
    [171]Russel P L and Oliver G. The effect of pH and NaCl content on starch gel ageing. A study by differential scanning calorimetry and theology [J]. Journal ofcereal science, 1989,10(4):123-138.
    [172]Sahlstrem S, Brathen E, Lea P, Autio K. Influence of starch granule size distribution on bread characteristics [J]. Journal of Cereal Science,1998,28(2):157-164.
    [173]Sanchez J C, Schaller D, Ravier F. Translationally controlled tumor protein:A protein identified in several nontumoral cells including erythrocytes [J]. Electrophorisis,1997, 18(1):150-155
    [174]Sandhu K S and Singh N. Some properties of corn starches II:physicoehemical, gelatinization, retrogradation, pasting and gel textural properties [J]. Food Chemistry, 2007101(4):1499-1507.
    [175]Sasaki T, Yasui T, Matsuki J. Effect of amylose content on gelatinization, retrogradation and pasting properties of starches from waxy and non-waxy wheat and their F1 seeds [J]. Cereal Chemistry,2000,77(1):58-63.
    [176]Satoh H, Nishi A, Yamashita K, Takemoto Y, Tanaka Y, Hosaka Y, Sakurai A, Fujita N, Nakamura Y. Starch-branching enzyme I-deficient mutation specifically affects the structure and properties of starch in rice endosperm [J]. Plant Physiology,2003,133(3): 1111-1121.
    [177]Schofield J D, Greenwell P. Wheat starch granule proteins and their technological significance [M]. In ID Morton, ed, Cereals in a European Context. Ellis Horwood, Chichester,1987, UK,407-420.
    [178]Sebecic B. Wheat flour starch granule-size distribution and theological properties of dough. I Granulomctrie analysis of starch [J]. Die Nahrong,1995,39(2):106-116.
    [179]Seib E W. Wheat starch, isolation, structure and properties [J]. Oy'o Toshitsu Kagaku, 1994,41(3):49-69.
    [180]Shinde S V, Nelson J E, Huber K C. Soft wheat starch pasting behavior in relation to A and B type granule content and composition [J]. Cereal Chemistry,2003,80(1): 91-98.
    [181]Sikka V K, Choi S, Kavakli I H, Sakulsingharoj C, Gupta S, Ito H, Okita T W. Subcellular compartmentation and allosteric regulation of the rice endosperm ADP glucose pyrophosphorylase [J]. Plant Science,2001,161(3):461-468.
    [182]Smith A M, Denyer K, Matin C R. The synthesis of the starch granule [J]. Annu Rev Plant Physiology Plant Molecular Biology,1997,48:67-87.
    [183]Singh J and Singh N. Studies on the morphological, thermal and rheological properties of starch separated from some Indian potato cultivars [J]. Food Chemistry,200175(1): 67-77.
    [184]Singh N, Singh J, Kaur L. Morphological, thermal and rheological properties of starches from different botanical sources [J]. Food Chemistry,2003,81(2):219-231.
    [185]Singh N, Kaur L, Sandhu K S, Kaur J, Nishinari K. Relationships between hysicochemical, morphological, thermal, rheological properties of fice starches [J]. Food Hydrocolloids 2006,20(5):532-542.
    [186]Singh N, Kaur M, Sandhu K S, Guraya H S. Physicochemical, Thermal, morphological and pasting properties of starches from some Indian black gram (Phaseolus mungo L.) [J]. cultivars. Starch/starke,2004,56(3):534-544.
    [187]Song Y and Jane J. Characterization of barley starches of waxy, normal, and high amylose varieties [J]. Carbohydrate Polymers,2000,41(2),365-377.
    [188]Soulaka A B and Merrison W R. The amylose and lipid contents, dimensions, and gelatinization characteristics of some wheat starches and their A-and B-type granules fractions [J]. Journal of Food and Agriculture Science,1985,36(1):709-718.
    [189]Stark D M, Timmerman K P, Barry G F, Preiss J, Kishore G M. Regulation of the amount of starch in plant tissues by ADP glucose pyrophosphrylase [J]. Cereal Science, 1992,258(4):287-292.
    [190]Stefan S, Anne B B, Robert G. Impact of Waxy, Partial Waxy, and Wildtype Wheat Starch Fraction Properties on Hearth Bread Characteristics [J]. Cereal Chemistry,2006, 83(5),647-654.
    [191]Steve J. Improving starch for food and industrial applications [J]. Plant Biology,2004, 7(1),210-218.
    [192]Stevens D J and Elton G A H. Thermal properties of starch/water system Ⅰ, measuremem of heat of gelatinization by differential scanning calorimetry [J]. Starch, 197123(4):8-11.
    [193]Steven G. B, Marion H B, VanW, Richard G F. Visser. Progress in understanding the biosynthesis of amylose [J]. Elsevier Plant Science,1998,3(12):1360-1385.
    [194]Stoddard F L. Survey of starch particle-size distribution in wheat and related species [J]. Cereal Chemistry,1999,76(10):145-149.
    [195]Suh D S and Jane J L. Comparison of starch pasting properties of various cooking conditions using the microviscoamylograph and the rapid visco-analyzer [J]. Cereal chemistry,2003,80(4):745-749.
    [196]Swinkels J J M. Composition and properties of commercial and native starches [J]. Starch,1985,37(6):1-5.
    [197]Takeda Y, Shibahara S, Hanashiro I. Examination of the structure of amylopeetin molecules by fluorescent labeling [J]. Carbohydrate Research,2003,338(5):471-475.
    [198]Tang H, Watanabe K, Mitsunaga T. Structure and functionality of large, medium and small granule starches in normal and waxy barley endosperms [J]. Carbohydrate Polymers,2002,49(3):217-224.
    [199]Tang H, Ando H, Watanabe K, Takeda Y and Mitsunaga T. Fine Structures of Amylose and Amylopectin from Large, Medium, and Small Waxy Barley Starch Granules. Cereal Chemistry,2001,78(6):111-115.
    [200]Tang H, Mitsunaga T, Kawamura Y. Relationship between functionality and structure in barley starches [J]. Carbohydrate Polymers,2004,57(4):145-152.
    [201]Tester R F and Morrison W R. Swelling and gelatinization of cereal starches I Effects of Amylopectin, amylase and lipids [J]. Cereal Chemistry,1990,67(1):551-556.
    [202]Tester R F, Karkalas J, Qi X. Starch-composition, fine structure and architecture [J]. Journal of Cereal Science,2004,39(3):151-165.
    [203]Tetlow I J, Davies E J, Vardy K A, Bowsher C G, Burrell M M, Ems M J. Subcellular localization of ADP glucose pyrophosphorylase in developing wheat endosperm and analysis of a plastidial isoform [J]. Journal of Experimental Botany,2003,54(1): 715-725.
    [204]Tian S J, Rickard J E, Blanshard J M V. Physico-chemical Properties of Sweet Potato Starch [J]. Journal of Science and Food Agriculture,1991,57(6):459-491.
    [205]Tsai C Y. The function of the waxy locus in starch synthesis in maize endosperm [J]. Biochemistry Genet,1974,11(3):83-96.
    [206]Toyokawa H, Rubenthaler G L, Powers J R, Schanus E G. Japanese noodle qualities. Ⅱ Starch components [J]. Cereal Chemistry,1989,66(5):387-391.
    [207]Turnbull K M and Rahraan S. Endosperm texture in wheat [J]. Journal of Cereal Science,2002,36(7):327-337.
    [208]Van Buynum G M A, Roels J A. Starch coversion technology [M]. New York:Marcel Dekker Inc,1985,3-4.
    [209]Vander Y E M and Bergsma J. Distribution of methyl substituents over crystalline and amorphous domains in methylated starches [J]. Carbohydrate Research,1999,320(10): 100-107.
    [210]Vidal M, Legrain P. Yeast forward and reverse'n'-hybrid systems [J]. Nucleic Acids Research,1999,27(4):919-929.
    [211]Visser R G F, Stolte A, Jacobsen E. Towards modifying plants for altered starch content and composition [J].Plant Science,1994,11(2):63-68.
    [212]Vriten P, Nakamura T. Wheat granule-bound starch synthesis I and II are endoded by separate genes that are expressed in different tissues [J]. Plant Physiology,2000,122(1): 255-263.
    [213]Wang A Y, Kao M H, Yang W H, Sayion Y, Liu L F, Lee P D, Su J C.Differentially and developmentally regulated expression of three rice sucrose synthase genes [J]. Plant Cell Physiology,1999,40(5):800-807.
    [214]Wang J, Jiang G, Vasanthan T, Sporns, P. MALDI-MS characterization of maltooligo /polysaccharides from debranched starch amylopectin of corn and barley [J]. Starke, 1999,51(3):243-249.
    [215]Wang X C, Li X F, Li Y X. A modified coomassie brilliant staining method at nangram sensitivity compatible with proteomic analysis [J]. Biotechnology letter,2007,29(3): 1599-1603.
    [216]Wang Y J, White P J, Pollack L. Thermal and gelling properties of maize mutants from OH43 inbred line [J]. Cereal Chemistry,1992,69(1):296-304
    [217]Wang Y J, White P, Pollack J L. Characterization of starch structure of 17 maize endosperm mutant genotypes with Oh43 inbred line background [J]. Cereal Chemistry, 1993,70(2):171-179.
    [218]Wang Z L, Yin Y P, He M R, Can H M. Source-sink manipulation effects on postanthesis photosynthesis and grain setting on spike in winter wheat [J]. Photosynthetica,1998,35(4):453-459.
    [219]Wang Z, Fu J, He M, Tian Q, Cao H. Effects of source/sink manipulation on net photosynthetic rate and photosynthate partitioning during grain filling in winter wheat [J]. Biologia Pinntamm,1997,39(8):379-385.
    [220]Wardlaw I F and Moncur L. The response of wheat to high temperature following anthesis./The rate and duration of kernel filling [J]. Plant Physiology,1995,22(6): 391-397.
    [221]Wasinger V C, Cordwell S J, Cerpa P C. Progress with geneproduct mapping of the mollic utes:Mycoplasma genitalium [J].Electrophoresis,1995,16(7):1090-1094.
    [222]William J H, Kent F M. Effect of temperature on expression of genes encoding enzymes for starch biosynthesis in developing wheat endosperm [J]. Plant Science,2003,164(5): 873-881.
    [223]Wilson, J D, Bechtel, D.B, Todd, T.C, Seib, P A. Measurement of wheat starch granule size distribution using image analysis and laser diffraction technology [J]. Cereal Chemistry,2006,83(3):259-268.
    [224]Wootton M, Panozzo I F, Hung S H. Differences in gelatinisation behaviour between starches from Australian wheat cultivars [J]. Starch,1998,50(4):154-158.
    [225]Xie Z J, Jiang.D. Effects of post-anthesis soil water status on the activities of key regulatory enzymes of starch and protein accumulation in wheat grains [J]. Journal of Plant Physiology and Molecular Biology,2003,29(4):309-316.
    [226]Yamin F F, Lee M, Pollak L M, White P J. Thermal properties of starch in corn variants isolated after chemical mutagenesis of inbred line B37 [J]. Cereal Chemistry,1999,76(2): 175-181.
    [227]Yang J C. Activities of key enzymes in sucrose to starch conversion in wheat grain subjected to water deficit during grain filling [J]. Plant Physiology,2004,135(6): 1621-1629.
    [228]Yan J X, Wait R, Berkelman T. Modified silver staining protocol for visualization of proteins compatible with matrix-assisted laser desorption/ionization and electrospray ionization mass spectrometry [J]. Electrophoresis,2000,21(10):3666-3672.
    [229]Yano H, Wong J H, Lee M. A strategy for the identification of proteins targeted by thioredoxin [J]. Proc Natl Acad Science,2001,98(8):4794-4799.
    [230]Yin Y, Wang Z, He M, Fu J, Lu S. Postanthesis allocation of photosynthates and grain growth in wheat cultivars as affected by source/sink change [J]. Biologia Plantarum, 1998,41:203-209.
    [231]Yoshimoto Y, Tashiro J, Takenouchi T, Takeda Y. Molecular structure and some physicochemical properties of lligh-amylose barley starch [J]. Cereal Chemistry,2000, 77:279-285.
    [232]Yoo S H and Jane J. Structural and physical characteristics of waxy and other wheat starches [J]. Carbohydrate Polymers,2002,49 (2):297-305.
    [233]Yuan R C, Thompson D B, Boyer C D. Fine structure of arnylopectin in relation to gelatinization and retrogradation behavior of maize starches from three Wax-containing genotypes in two inbred lines [J]. Cereal Chemistry,1993,70 (5):81-89.
    [234]Zeng M, Morris C F, Batey I L. Sources of variation for starch gelatinization, pasting and gelation properties in wheat [J]. Cereal Chemistry,1997,74 (1):63-71.
    [235]Zhao J, Madson M A, Whistler R L.Cavities in porous corn starch provide a large storage space [J].Cereal Chemistry,1996,73 (5):379-380.
    [236]Zihua A, Jay J. Characterization and modeling of the A- and B-granule starches of wheat, triticale, and barley [J]. Carbohydrate Polymers,2007,67(4):46-55.
    [237]Zobel H F, Young S N, Rocca L A. Starch gelatinization:an X-ray diffraction study [J]. Cereal Chemistry,1988,65(2):443-446.
    [238]Zhou M, Robards K, Glennie-Holmes, Helliwell S. Structure and Pasting Properties of Oat Starch [J]. Cereal Chemistry,1998,75(3):273-281.

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