禽流感H5亚型、结核分支杆菌及弓形虫感染金标快速检测技术研究
|
摘要
目前,由各种病原微生物引起的感染性疾病仍然是危害人类健康、导致人类和动物死亡的重要原因。其中禽流感、结核等病原体引起的感染性疾病呈爆发流行,引发重大的社会和经济问题。弓形虫病等人兽共患病在严重威胁畜牧业发展的同时,也可引起人口出生健康等问题。研究病原微生物的新型检测技术及试剂盒产品,对感染性疾病的防控具有重要意义。本论文采用单克隆抗体技术、基因工程技术、胶体金标记及免疫层析技术,建立了禽流感H5亚型病毒(AIV-H5)、结核及弓形虫感染金标快速检测技术并研制了试剂盒。
用AIV-H5抗原免疫BALB/c小鼠,取小鼠脾脏与SP2/0骨髓瘤细胞融合,筛选AIV-H5单克隆抗体(McAb),测定抗体亚类并分析其抗原结合位点。结果共获得5株单克隆抗体,均具有良好的型特异性。除了一株抗体亚类为IgM外,其余4株均为IgG,腹水抗体效价最高可达1:2.56×10~5,其中三株单抗具有不同的抗原结合位点,适合“双抗体夹心”免疫反应。用一株胶体金标记的AIV-H5 McAb和另一株未标记AIV-H5 McAb包被硝酸纤维素膜,制备金标检测试剂盒。结果与AIV-H5抗原反应较好,与AIV其它亚型及NDV,IBV,EDSV等禽类病毒不发生交叉反应。检测灵敏度可达到1:2~2血凝( HA)效价单位,比琼脂扩散试验高10倍,但比荧光免疫组化法低10倍。检测试剂盒的符合率、重复性均达到100%,紧密型性CV值为0,37℃12d破坏实验表明试剂盒质量稳定。除心脏检测为阴性外,试剂盒对人工感染鸡的肝、脾、肺等脏器的检测结果与鸡胚病毒分离培养100%符合。田间试验结果与荧光RT-PCR试剂盒符合率为100%。
根据结核分枝杆菌TB16和TB38基因序列设计引物,以结核分枝杆菌(H37Rv)基因组DNA为模板进行PCR扩增,分别克隆TB16和TB38基因片段。用限制性内切酶酶切后分别与pET-30a质粒连接再转入E. coli DH5α感受态细胞,挑取转化菌落提取质粒进行PCR、酶切及测序鉴定。将构建的重组表达质粒pET30a-TB16和pET30a-TB38转化E. coli BL21(DE3),诱导表达后用SDS-PAGE进行分析。结果PCR扩增分别获得约0.4 kb的TB16基因片段和约1.1 kb的TB38基因片段。成功构建了pET30a-TB16和pET30a-TB38重组表达质粒,测序结果表明插入的TB16和TB38目的基因片段分别为435 bp和1053 bp,与GenBank中的相关基因序列完全相同。pET30a-TB16转化菌经IPTG诱导后,在相对分子量21 kDa处有表达带出现,重组蛋白主要以可溶性形式表达,表达量占菌体总蛋白的40%左右。pET30a-TB38转化菌经IPTG诱导后,在相对分子量41 kD处有表达带出现,重组蛋白主要以包涵体形式表达,表达量占菌体总蛋白的30%左右。应用TB16和TB38重组蛋白作检测抗原包被硝酸纤维素膜,研制了结核抗体金标阵列检测试剂盒。结果对结核抗体阴阳性质控品检测符合率均达到100%,紧密性CV值为0,37℃12d破坏实验表明试剂盒质量稳定。检测活动性肺结核病人血清抗体的灵敏度为78.3%,特异性为93%,其敏感性明显高于抗酸染色(27.96%)和细菌培养(57.99%),差异极显著(P﹤0.01),比单用TB38重组蛋白为检测抗原的结核抗体金标检测卡(72.2%)高6.1%。检测抗酸染色阴性病例,结核抗体阳性率达到77.66%,检出率接近阳性病例(82.32%)。检测结核分枝杆菌培养阴性病例,结核抗体阳性率亦可达到65.5%,但检出率不如阳性病例(85.82%)。
应用弓形虫分泌抗原制备了“双抗原夹心”弓形虫抗体金标检测卡。结果对弓形虫抗体阴阳性质控品检测符合率均达到100%,紧密性CV值为3%,37℃12d破坏试验和常温保存18个月,检测卡质量保持不变。对852份健康体检人血清、547份孕、产妇血清以及1206份各种肿瘤病人血清样品检测的阳性率分别为2.87%,4.38%和6.72%,与ELISA符合率达96%。弓形虫在肿瘤患者中的感染情况显示,肿瘤患者弓形虫阳性率高于正常体检人群2.38倍(肿瘤患者6.72%,正常体检人2.82%),两者相比差异显著(P<0.01)。不同临床类型恶性肿瘤患者弓形虫感染率不同,以肾癌患者最高(22.50%),其次为肺癌(15.04%)、皮肤癌(12.05%)和乳腺癌(9.03%)患者,高于正常人的感染率(2.82%)3-8倍,差异极显著(P<0.01);消化道癌患者感染率(5.56%)与正常人相比差异显著(P<0.05);子宫癌(3.70%)和其他恶性肿瘤(3.68%)感染率略高于正常人,但无显著性差异(P﹥0.05),而血液淋巴系统恶性肿瘤(2.27%)、肝癌(1.79%)和甲状腺癌(0)感染率低于正常人。不同临床类型肿瘤患者之间弓形虫感染率差异较大,最高感染者肾癌是其他恶性肿瘤患者平均感染率(6.72%)的3.3倍,两者之间有极显著差异性(P<0.01)。
本研究研制的AIV-H5金标检测试剂盒、结核抗体金标阵列检测试剂盒及弓形虫抗体金标检测卡特异性强、敏感性高,可适用于H5亚型禽流感、结核病及弓形虫病的检测、监控和临床诊断,是具有广泛应用价值的快速检测试剂。
At present, the infectious diseases caused by a variety of pathogenic microorganisms continue to be hazardous to human health and lead to death of humans and animals. Outbreaks of avian influenza, tuberculosis and other infectious diseases have triggered major social and economic problems. Toxoplasma gondii infection and other zoonotic diseases have been a serious threat to the development of animal breeding and cause human health problems at birth. The development of new detection techniques and kits is important for infectious disease prevention and treatment. In this paper, rapid detection techniques and kits for the avian influenza virus H5 subtype (AIV-H5), Mycobacterium tuberculosis and Toxoplasma gondii infection were developed based on the monoclonal antibody technique, genetic engineering technique and colloidal gold labeling immunochromatography technique.
BALB/c mice were immunized with purified AIV-H5 antigen and spleen cells were fused to myeloma cells SP2/0. The AIV-H5 monoclonal antibodies were screened and the Ig subclass and their antigen binding sites were analysed. Five AIV-H5 monoclonal antibodies with good specificity and stability were obtained. Except for one McAb which belongs to IgM subclass, the other four are IgG subclass with the highest ascites antibody titer of 1:2.56×105. Three of them have different antigen binding sites and are suitable for“double-antibody sandwich”immunoassay. AIV-H5 lateral flow assay was developed by colloidal gold labeling AIV-H5 McAb and AIV-H5McAb coated on nitrocellulose membrane. Except for AIV-H5 antigen, the developed lateral flow assay did not have cross reaction with the other AIV subtypes, NDV, IBV, EDSV or other avian viruses. The sensitivity reached 1: 22 HA (hemocyte agglutination) which was 10 times higher than that of agar diffusion test but 10 times lower than the fluorescence immunohistochemistry assay. The coincidence rate and reproducibility of the assay is 100% with precision coefficient of variation of zero (CV=0). The kit quality was stable after store at 37℃for 12 days. Except for the negative result from the heart, AIV-H5 was detected in the liver, spleen, lung and other organs of chicken artificially infected with AIV-H5 by this assay, which is 100% coincident to chicken embryo virus isolation assay. In field test the coincidence rate of the assay and fluorescent RT-PCR kit was 100%.
The primers derived from TB16 and TB38 genes were used to amplify the target gene fragments from Mycobacterium tuberculosis H37Rv genomic DNA. After digested with restriction enzymes, the amplified TB16 and TB38 gene fragments were ligated to pET30a and transformed to E. coli DH5αcell. The transformants were identified by PCR, enzyme digestion and sequencing. The 0.4 kb fragment of TB16 and 1.1 kb fragment of TB38 were amplified. The pET30a-TB16 and pET30a-TB38 recombinant expression plasmids were successfully constructed and sequencing results indicated the 435 bp insert of TB16 and 1053 bp insert of TB38 were identical to the corresponding sequences in GenBank. The recombinant plasmid pET30a-TB16 and pET30a-TB38 were then transformed to E. coli BL21 (DE3), induced for expression and analysed with SDS-PAGE. After induction by IPTG, pET30a-TB16 transformant had an expression band at 21 kDa which reached 40% of bacterial total protein as soluble form. After induction by IPTG, pET30a-TB38 transformant had an expression band at 41 kDa which reached 30% of bacterial total protein as inclusion body form. Tuberculosis antibody colloidal gold labeling array assay was developed by colloidal gold labeling human IgG McAb with TB16 and TB38 recombinant antigens coated on nitrocellulose membrane. The detection coincidence rate and reproducibility of the assay is 100% with precision coefficient of variation of of zero (CV=0). The kit quality was stable after store at 37℃for 12 days. The sensitivity for detection active TB antibody was 78.3%, which was higher than acid-fast stain (27.96%) and Bacteria culture (57.99%) with very significant difference (P<0.01). The sensitivity was 6.1% higher than that of TB antibody lateral flow assay based on only TB38 recombinant antigen. TB antibody positive rate of the array assay was 77.66% in acid-fast stain negative patients and the detection rate was similar to positive patients (82.32%). TB antibody positive rate of the array assay was 65.50% in Bacteria culture negative patients but the detection rate was lower than positive patients (85.82%).
Double-antigen sandwich T. gondii antibody colloidal gold labeling lateral flow assay was developed with T. gondii excretory and secretory antigen. The detection coincidence rate of the assay is 100% to T. gondii positive and negative control serum with the precision coefficient of variation of 0.3% (CV=0.3%). The kit quality was stable after storage at 37℃for 12 days or at room temperature for 18 months. The T. gondii antibody positive rate was 2.87%, 4.38% and 6.72% respectively in 852 normal physical examination serum samples, 547 pregnant or lying-in woman serum samples and 1206 tumor patients, which was 96% identical to that of ELISA. T. gondii infection in tumor patients indicated that T. gondii antibody positive rate in tumor patients (6.72%) was 2.38 times higher than that in normal medical population (2.82%) with very significant difference (P<0.01). There were some differences of T. gondii infection rates in different clinical forms tumor patients. T. gondii infection rate in the renal cell carcinoma patients was the highest (22.50%), followed by lung cancer (15.04%), skin cancer (12.05%) and breast cancer (9.03%) patients, which was 3 to 8 times higher than those in normal human serum (2.82%) with very significant difference (P<0.01). T. gondii infection rate in digestive tract cancer patients was 5.56% with significant difference to normal serum (P<0.05). T. gondii infection rate in uterine cancer patients was 3.70% and 3.68% in other malignant tumor patiens which was slightly higher than normal serum with no significant difference (P>0.05). While the T. gondii infection rate in blood lymphoid malignancies (2.27%), liver (1.79%) and thyroid cancer (0) was lower than those in normal serum. T. gondii infection rates were different among different clinical forms of tumor patients, the infection in renal cell carcinoma was 3.3 times higher than the average infection rate in other malignant tumors (6.72%) with very significant differences (P <0.01) .
The developed AIV-H5 gold labeling detection kit, TB antibody gold labeling array assay kit and T. gondii antibody gold labeling lateral flow assay kit showed high specificity and sensitivity and could be used to rapidly detect these diseases.
用AIV-H5抗原免疫BALB/c小鼠,取小鼠脾脏与SP2/0骨髓瘤细胞融合,筛选AIV-H5单克隆抗体(McAb),测定抗体亚类并分析其抗原结合位点。结果共获得5株单克隆抗体,均具有良好的型特异性。除了一株抗体亚类为IgM外,其余4株均为IgG,腹水抗体效价最高可达1:2.56×10~5,其中三株单抗具有不同的抗原结合位点,适合“双抗体夹心”免疫反应。用一株胶体金标记的AIV-H5 McAb和另一株未标记AIV-H5 McAb包被硝酸纤维素膜,制备金标检测试剂盒。结果与AIV-H5抗原反应较好,与AIV其它亚型及NDV,IBV,EDSV等禽类病毒不发生交叉反应。检测灵敏度可达到1:2~2血凝( HA)效价单位,比琼脂扩散试验高10倍,但比荧光免疫组化法低10倍。检测试剂盒的符合率、重复性均达到100%,紧密型性CV值为0,37℃12d破坏实验表明试剂盒质量稳定。除心脏检测为阴性外,试剂盒对人工感染鸡的肝、脾、肺等脏器的检测结果与鸡胚病毒分离培养100%符合。田间试验结果与荧光RT-PCR试剂盒符合率为100%。
根据结核分枝杆菌TB16和TB38基因序列设计引物,以结核分枝杆菌(H37Rv)基因组DNA为模板进行PCR扩增,分别克隆TB16和TB38基因片段。用限制性内切酶酶切后分别与pET-30a质粒连接再转入E. coli DH5α感受态细胞,挑取转化菌落提取质粒进行PCR、酶切及测序鉴定。将构建的重组表达质粒pET30a-TB16和pET30a-TB38转化E. coli BL21(DE3),诱导表达后用SDS-PAGE进行分析。结果PCR扩增分别获得约0.4 kb的TB16基因片段和约1.1 kb的TB38基因片段。成功构建了pET30a-TB16和pET30a-TB38重组表达质粒,测序结果表明插入的TB16和TB38目的基因片段分别为435 bp和1053 bp,与GenBank中的相关基因序列完全相同。pET30a-TB16转化菌经IPTG诱导后,在相对分子量21 kDa处有表达带出现,重组蛋白主要以可溶性形式表达,表达量占菌体总蛋白的40%左右。pET30a-TB38转化菌经IPTG诱导后,在相对分子量41 kD处有表达带出现,重组蛋白主要以包涵体形式表达,表达量占菌体总蛋白的30%左右。应用TB16和TB38重组蛋白作检测抗原包被硝酸纤维素膜,研制了结核抗体金标阵列检测试剂盒。结果对结核抗体阴阳性质控品检测符合率均达到100%,紧密性CV值为0,37℃12d破坏实验表明试剂盒质量稳定。检测活动性肺结核病人血清抗体的灵敏度为78.3%,特异性为93%,其敏感性明显高于抗酸染色(27.96%)和细菌培养(57.99%),差异极显著(P﹤0.01),比单用TB38重组蛋白为检测抗原的结核抗体金标检测卡(72.2%)高6.1%。检测抗酸染色阴性病例,结核抗体阳性率达到77.66%,检出率接近阳性病例(82.32%)。检测结核分枝杆菌培养阴性病例,结核抗体阳性率亦可达到65.5%,但检出率不如阳性病例(85.82%)。
应用弓形虫分泌抗原制备了“双抗原夹心”弓形虫抗体金标检测卡。结果对弓形虫抗体阴阳性质控品检测符合率均达到100%,紧密性CV值为3%,37℃12d破坏试验和常温保存18个月,检测卡质量保持不变。对852份健康体检人血清、547份孕、产妇血清以及1206份各种肿瘤病人血清样品检测的阳性率分别为2.87%,4.38%和6.72%,与ELISA符合率达96%。弓形虫在肿瘤患者中的感染情况显示,肿瘤患者弓形虫阳性率高于正常体检人群2.38倍(肿瘤患者6.72%,正常体检人2.82%),两者相比差异显著(P<0.01)。不同临床类型恶性肿瘤患者弓形虫感染率不同,以肾癌患者最高(22.50%),其次为肺癌(15.04%)、皮肤癌(12.05%)和乳腺癌(9.03%)患者,高于正常人的感染率(2.82%)3-8倍,差异极显著(P<0.01);消化道癌患者感染率(5.56%)与正常人相比差异显著(P<0.05);子宫癌(3.70%)和其他恶性肿瘤(3.68%)感染率略高于正常人,但无显著性差异(P﹥0.05),而血液淋巴系统恶性肿瘤(2.27%)、肝癌(1.79%)和甲状腺癌(0)感染率低于正常人。不同临床类型肿瘤患者之间弓形虫感染率差异较大,最高感染者肾癌是其他恶性肿瘤患者平均感染率(6.72%)的3.3倍,两者之间有极显著差异性(P<0.01)。
本研究研制的AIV-H5金标检测试剂盒、结核抗体金标阵列检测试剂盒及弓形虫抗体金标检测卡特异性强、敏感性高,可适用于H5亚型禽流感、结核病及弓形虫病的检测、监控和临床诊断,是具有广泛应用价值的快速检测试剂。
At present, the infectious diseases caused by a variety of pathogenic microorganisms continue to be hazardous to human health and lead to death of humans and animals. Outbreaks of avian influenza, tuberculosis and other infectious diseases have triggered major social and economic problems. Toxoplasma gondii infection and other zoonotic diseases have been a serious threat to the development of animal breeding and cause human health problems at birth. The development of new detection techniques and kits is important for infectious disease prevention and treatment. In this paper, rapid detection techniques and kits for the avian influenza virus H5 subtype (AIV-H5), Mycobacterium tuberculosis and Toxoplasma gondii infection were developed based on the monoclonal antibody technique, genetic engineering technique and colloidal gold labeling immunochromatography technique.
BALB/c mice were immunized with purified AIV-H5 antigen and spleen cells were fused to myeloma cells SP2/0. The AIV-H5 monoclonal antibodies were screened and the Ig subclass and their antigen binding sites were analysed. Five AIV-H5 monoclonal antibodies with good specificity and stability were obtained. Except for one McAb which belongs to IgM subclass, the other four are IgG subclass with the highest ascites antibody titer of 1:2.56×105. Three of them have different antigen binding sites and are suitable for“double-antibody sandwich”immunoassay. AIV-H5 lateral flow assay was developed by colloidal gold labeling AIV-H5 McAb and AIV-H5McAb coated on nitrocellulose membrane. Except for AIV-H5 antigen, the developed lateral flow assay did not have cross reaction with the other AIV subtypes, NDV, IBV, EDSV or other avian viruses. The sensitivity reached 1: 22 HA (hemocyte agglutination) which was 10 times higher than that of agar diffusion test but 10 times lower than the fluorescence immunohistochemistry assay. The coincidence rate and reproducibility of the assay is 100% with precision coefficient of variation of zero (CV=0). The kit quality was stable after store at 37℃for 12 days. Except for the negative result from the heart, AIV-H5 was detected in the liver, spleen, lung and other organs of chicken artificially infected with AIV-H5 by this assay, which is 100% coincident to chicken embryo virus isolation assay. In field test the coincidence rate of the assay and fluorescent RT-PCR kit was 100%.
The primers derived from TB16 and TB38 genes were used to amplify the target gene fragments from Mycobacterium tuberculosis H37Rv genomic DNA. After digested with restriction enzymes, the amplified TB16 and TB38 gene fragments were ligated to pET30a and transformed to E. coli DH5αcell. The transformants were identified by PCR, enzyme digestion and sequencing. The 0.4 kb fragment of TB16 and 1.1 kb fragment of TB38 were amplified. The pET30a-TB16 and pET30a-TB38 recombinant expression plasmids were successfully constructed and sequencing results indicated the 435 bp insert of TB16 and 1053 bp insert of TB38 were identical to the corresponding sequences in GenBank. The recombinant plasmid pET30a-TB16 and pET30a-TB38 were then transformed to E. coli BL21 (DE3), induced for expression and analysed with SDS-PAGE. After induction by IPTG, pET30a-TB16 transformant had an expression band at 21 kDa which reached 40% of bacterial total protein as soluble form. After induction by IPTG, pET30a-TB38 transformant had an expression band at 41 kDa which reached 30% of bacterial total protein as inclusion body form. Tuberculosis antibody colloidal gold labeling array assay was developed by colloidal gold labeling human IgG McAb with TB16 and TB38 recombinant antigens coated on nitrocellulose membrane. The detection coincidence rate and reproducibility of the assay is 100% with precision coefficient of variation of of zero (CV=0). The kit quality was stable after store at 37℃for 12 days. The sensitivity for detection active TB antibody was 78.3%, which was higher than acid-fast stain (27.96%) and Bacteria culture (57.99%) with very significant difference (P<0.01). The sensitivity was 6.1% higher than that of TB antibody lateral flow assay based on only TB38 recombinant antigen. TB antibody positive rate of the array assay was 77.66% in acid-fast stain negative patients and the detection rate was similar to positive patients (82.32%). TB antibody positive rate of the array assay was 65.50% in Bacteria culture negative patients but the detection rate was lower than positive patients (85.82%).
Double-antigen sandwich T. gondii antibody colloidal gold labeling lateral flow assay was developed with T. gondii excretory and secretory antigen. The detection coincidence rate of the assay is 100% to T. gondii positive and negative control serum with the precision coefficient of variation of 0.3% (CV=0.3%). The kit quality was stable after storage at 37℃for 12 days or at room temperature for 18 months. The T. gondii antibody positive rate was 2.87%, 4.38% and 6.72% respectively in 852 normal physical examination serum samples, 547 pregnant or lying-in woman serum samples and 1206 tumor patients, which was 96% identical to that of ELISA. T. gondii infection in tumor patients indicated that T. gondii antibody positive rate in tumor patients (6.72%) was 2.38 times higher than that in normal medical population (2.82%) with very significant difference (P<0.01). There were some differences of T. gondii infection rates in different clinical forms tumor patients. T. gondii infection rate in the renal cell carcinoma patients was the highest (22.50%), followed by lung cancer (15.04%), skin cancer (12.05%) and breast cancer (9.03%) patients, which was 3 to 8 times higher than those in normal human serum (2.82%) with very significant difference (P<0.01). T. gondii infection rate in digestive tract cancer patients was 5.56% with significant difference to normal serum (P<0.05). T. gondii infection rate in uterine cancer patients was 3.70% and 3.68% in other malignant tumor patiens which was slightly higher than normal serum with no significant difference (P>0.05). While the T. gondii infection rate in blood lymphoid malignancies (2.27%), liver (1.79%) and thyroid cancer (0) was lower than those in normal serum. T. gondii infection rates were different among different clinical forms of tumor patients, the infection in renal cell carcinoma was 3.3 times higher than the average infection rate in other malignant tumors (6.72%) with very significant differences (P <0.01) .
The developed AIV-H5 gold labeling detection kit, TB antibody gold labeling array assay kit and T. gondii antibody gold labeling lateral flow assay kit showed high specificity and sensitivity and could be used to rapidly detect these diseases.
引文
[1] Chinese SMEC. Molecular evolution of the SARS coronavirus during the course of the SARS epidemic in China. [J]. Science 2004, 303(5664):1666-1669
[2] Le QM, Kiso M, Someya K, et al. Avian flu: isolation of drug-resistant H5N1 virus. [J]. Nature 2005, 437(7062):1108
[3] Mebride MT,Gammon S,Pitesky M,et al. Multiplexed liquid arrays for simultaneous detection of simulants of biological warfare agents. [J]. Anal Chem,2003,75(8):1924-1930
[4]李卫华.食品微生物快速检测方法进况.现代商务科技.[J].1998, 3(8) :53-56
[5]商检行业标准.SN0133-94.出口食品中大肠杆菌(葡萄糖酶荧光)检验方法.1994
[6]甄宏太,杨素贤,刘中学,等.应用MUCAP试剂快速检测沙门氏菌.[J].微生物学报.1996 , 36 (1) : 586-592.
[7]陆茂林,蔡建荣,赵晓联,等.标记免疫分析技术在食品分析中的应用.[J].中国卫生检验杂志. [J].2002,12(2):60-62
[8]龙军,陈清,俞守义.双抗体夹心ELISA法检测葡萄球菌B型肠毒素.[J].中国公共卫生.2005,21(2):154-155
[9]涂少华,叶元康,沈昭在.双抗体夹心ELISA检测肺炎支原体抗原方法的建立.[J].检验医学, 2005 , 20 (3) :214-216.
[10] Gehring AG, Irwin PL, Reed SA, et al. Enzyme-linked immunomagnetic chemiluminescent detection of E.coli O157:H7 .[J].Immunol Meth, 2004, 293(12): 97-106
[11]吕治林.两种成分联合的直接荧光抗体技术对细菌性炭疽的快速诊断.[J].国外医学.流行病学.传染病学分册.2003 , 1(30) :58.
[12] Mata AI, Gibello A, Casamayor A, et al. Multiplex PCR assay for detection of bacterial pathogens with associated warm-water streptococcosis in fish.[J].Appl Environ Microbiol. 2004, 70 (5) :3183-3187.
[13] Glukhov AI, Gordeev SA, Al’tshuler ML, et al. Study of the effectiveness of the method of the nest polymerase chain reaction and the field of its use in hepatitis A diagnosis.[J]. Zh Mikrobiol Epidemiol Immunobiol 2004 , 6: 88-91.
[14] Boutaga K, van Winkelhoff AJ, and enbroucke-Grauls CM ,et al . Comparison of real-time PCR and culture for detection of porphyromonas gingivalis in subgingival plaque samples. [J]. Clin Microbiol , 2003 ,41 (11) : 4950-4954.
[15]Rehman FN, Audeh M, Abrams ES, et al. Immobilization of acrylamide-modified oligonucleotides by co-polymerization .[J].Nucleic Acids Res. 1999, 27(2):649-655.
[16] Sano T, Smith CL, and Cantor CR. Immuno-PCR: very sensitive antigen detection by means of specific antibody-DNA conjugates.[J].Science.1992, 258(5079): 120-122.
[17] Niemeyer CM , Adler M , Wacker R. Immuno-PCR: high sensitivity detection of proteins by nucleic acid amplification.[J].Trends Biotechnol , 2005 ,23 (4) : 208-216
[18]翟新验,卢胜明,刘钧.实时TaqMan-PCR技术在兽医学上的应用.[J].实验动物科学与管理,2002,6(2):19-22
[19] Munch M, Nielsen LP, Handberg KJ , et al . Detection and subtyping (H5 and H7) of avian type A influenza virus by reverse transcription-PCR and PCR ELISA .[J]. Arch Virol. 2001,146 (1) : 87-97.
[20]林万明主编,临床基因探针诊断实验技术.[M].上海科学技术出版社, 1993
[21] Burton W.B, Lucille M.P. A simple RNA probe system for analysis of Listeriamonocytogenes polymerase chain reaction products .[J]. Applied and Environmental Microbiology. 1993, 59 (9):2795-2800
[22] Amann RI, Krumholz L, Stahl DA. Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in micro- biology .[J].Bacteriol.1990, 172:762-770.
[23]Mahmoud KK,Leduc LG,Ferroni GD. Detection of Acidithiobacillus ferrooxidans in acid mine drainage environments using fluorescent in situ hybridization (FISH).[J]. Microbiol Methods. 2005,61 (1):33-45.
[24]葛晶,殷涌光,王凯.使用SPR生物传感器快速检测大肠杆菌E.coli O157:H7.[J].吉林大学学报(工学版). 2005, 35(2): 214-218.
[25] Masarova J, Dey ES, and Danielsson B. Optical lectin based biosensor as tool for bacteria identification.[J]. Clin Microbiol. 2004, 53: 23-27
[26]邱景富,王升启.生物芯片技术及其在病原微生物检测方面的进展.[J].中国微生态学杂志.2003,15(2):124
[27] Anthony RM ,Brown TJ, French GL.Rapid diognosis of bacteria by universal amp lification of 23S ribosomal DNA followed by hybridization to an oligonucleo- tide array. [J].Clin Microbiol, 2000, 38(20) : 781-788
[28] Jack S, Dougles RC, F red JB, et al. Direct detection of 16S RNA in soil extracts by using oligonulrotide microarrays .[J].App Env Microbiol, 2001, 67(10): 4708-4716
[29] Zhang Llin-qi, YuWen-jie, He Tian,et al. Contribution of human alpha-defensin 1, 2, and 3 to the anti-HIV-1 activity of CD8 antiviral factor.[J]. Science. 2002, 298: 995-1000.
[30] KangXi-xiong, XuYang, Wu Xiao-y,ietal. Establish of the proteomic fingerprints for potential application to early diagnosis of severe acute respiratory syndrome (SARS).[J]. Clin Chem. 2005, 51: 56-64
[31] R.G. McClelland, Detection of Salmonella typhimurium in dairy products with flow cytometry and monoclonal antibodies.[J]. Appl Environ Microbiol.1994, 60(12): 4255-4262
[32] Bio merieux公司产品介绍
[33]刘敏,汤一苇. IbisT5000:一种新型病原微生物检测仪.[J].微生物与感染. 2008,3(4):254-255
[34] Mcdade RL, Spain MD. Rapid, economical testing in the clinical laboratiory: a new flow cytometry-based multiplex system .[J]. Clin Irnmunol Newsletter. 1997, 17(10): 154-158
[35]胡瑞,赵金银,陈德坤等.应用xMAP液态芯片多重快速检测四种病原微生物的研究.[J].现代生物医学进展. 2008,8(12):2232-2235
[36]陆学东,周一平,杨来智等.多种呼吸道病原微生物快速筛查技术的建立.[J].中华医院感染学杂志.2008,18(1):140-143
[37]鲁卫平,顾大勇,王华等.基于纳米金的显色基因芯片快速检测病原微生物.[J].生物技术通报.2008,3:95-98
[38]张如胜.LAMP技术在病原微生物检测中的应用.[J].华南预防医学.2007,33(5): 45-49
[39] Romano El, et al.[M].Historical aspects. Academic Press. Amsterdam New. Oxford, 1984.
[40] Horisberger M, Rosset. Colloidal gold, a useful marker for transmission and scanning electron microscopy .[J].Histochem .Cytochem. 1977,25(4):295-305.
[41] Stathis. E.C .[M]. Fabrikanos A. Chem. Ind.1958,27:860.
[42] Hirisberger M, Tacchini-Vonlanthen M. Ultrastructural localization of Kunitz inhibitor on thin sections of Glycine max (soybean) cv. Maple Arrow by the gold method.[J].Hisiochemistry.1983,77(1):37-50.
[43] Fren G. Controlled Nucleation for the Regulation of the Particle Size in Monodisperse Gold Suspensions.[J].Nature, Phys Sci .1973,241:20.
[44] Horisberger M. Evaluation of colloidal gold as a cytochemical marker for transmission and scanning electron microscopy.[J]. Biol Cell. 36:253-259.
[45] Baigent C. L. and G. Muller. A colloidal gold prepared with ultrasonics.[J]. Experientia. 1980,36:472-473.
[46] Tschopp J. Podack ER, Müller-Eberhard HJ. Ultrastructure of the membrane attack complex of complement: Detection of the tetramolecular C9-polymerizing complex C5b-8.[J]. Proc Natl Acad Sci.USA,1982,79(23):7474-7478.
[47] Slot JW. A method to prepare isodisperse colloidal gols sols in size range 3-17nm . [J]. Ultramicroscopy.1985,15:383.
[48] Slot JW,Geuze HJ. Sizing of protein A-colloidal gold probes for immunoelectron microscopy .[J].Cell Biol.1981,90:533-536.
[49] De Mey J, Moeremans M, Geuens G, et al. High resolution light and electron microscopic localization of tubulin with the IGS (immuno gold staining) method .[J]. Cell Biol. 1981 ,5(9):889–899
[50] Faulk WP, Taylar G.M. An immunocolloidal method for the electron microscope. [J].Immunochemistry. 1971, 8:1081-1084
[51] Hodges GM, Smolira MA, Livingston D. Scanning Electron Microscope Immunocytochemistry in Practice: Immunolabelling For Electron Microscopy. [M]. Elsevier Science Publishers BV, Amsterdam, 189-233.
[52]Slot JW. et al.[M]. Immunolabelling For Electron Microscopy Press. New York, 1984.129-142
[53] Singer S.J, Tokuyasu K. T, Gilbert-A. et al.Immunoelectron Microscopy and the Molecular Ultanstructure of Cells .[J].Electron Microsc.1987,36(3);63-71
[54] Feldherr C.M, and Marshall J. The use of colloidal gold for studies of intracellular exchanges in the ameba Chaos chaos.[J]. Cell Biol. 1962,12:640–645
[55] Valkirs GE,Barton R. lmmuno concentration--a new format for solid-phase immunoassays.[J]. Clin Chem,1985,31:1427-1431.
[56] Spielberg F, Kabeya CM, Ryder RW, et al. Field testing and comparative cvaluation of rapid, visually read screening assays for antibody to human immunodeficiency virus. .[J]. Lancet.1989,1:580-584
[57]沈丽英,干小仙,丁建祖等.检测幽门螺杆菌抗体的金标免疫渗滤法的建立及应用.[J].浙江实用医学,1997,(4):16-17
[58]陈国庆,古洁若,陆才生等.金标免疫渗滤法检测抗角质蛋白抗体在类风湿性关节炎诊断中的意义.[J].广东医学.2003,(10):1093-1094
[59]陈国庆,古洁若,黄建林等. A组溶血性链球菌膜抗原吸附心肌抗体金标免疫渗滤法的建立.[J].现代检验医学杂志,2003,(5):7-8
[60]沈丽英,干小仙,丁建祖等.金标免疫渗滤法(DIGFA)快速检测血吸虫循环抗原的研究[J].中国人兽共患病杂志.2000,(1):12-14。
[61]施晓华,汤益,王越等.成虫三氯醋酸抽提抗原金标免疫渗滤法诊断日本血吸虫病及考核疗效的研究.[J].中国血吸虫病防治杂志.2006,(2):116-118
[62]顾炜峰,王越,干小仙.金标免疫渗滤法检测猫狗血清中抗肺吸虫IgG..[J].医学动物防制.2008,(2):119-120
[63] Beggs.M, Novotny.M, Sampedron.S,et al.A selfperforming chromatographic immunoassay for the qualitive detemination of human chorionic gonadtrophin (HCG) in urine and serum. [J].Clinchem, 1990, 36:1084-1085.
[64]周继文,戎广亚,扬守纯等,胶体金免疫层析法检测乙型肝炎病毒表面抗原[J].中华医学检验杂志.1998,21(1):30-32.
[65]吴守丽,严延生,李世清等.重组汉坦病毒核衣壳蛋白抗原用于胶体金标记免疫层析法检测肾综合征出血热抗体.[J].中华微生物学和免疫学杂志.2007,27(2): 192-196
[66]陈军政.免疫胶体金法快速检测轮状病毒腹泻的结果分析.[J].现代预防医学.2007,(9):1763-1764
[67]胡凤娟.胶体金法筛查丙型肝炎抗体在街头献血车中的应用.[J].检验医学,2007,(4):516
[68]蒋韬,梁仲,陈涓等.检测AsiaⅠ型口蹄疫病毒的胶体金免疫层析法的建立及应用.[J].细胞与分子免疫学杂志.2007,(11):1021-1024
[69]程庆,刘志伟,郑定容等.胶体金法在HIV抗体检测中的应用.[J].实用医技杂志. 2008,(17):2207-2208
[70]何艳玲,林松,王陆迪等.体金免疫层析法检测水产品中O_1群霍乱弧菌方法的建立与优化.[J].中国国境卫生检疫杂志.2007,(1):52-55
[71]刁琳琪,王伟,徐建国等.胶体金标记免疫层析法检测大肠杆菌O157.[J].疾病监测.2007.(7):438-440
[72]赵晶,姜海燕,杜民等.毒品胶体金试剂快速测试仪的研制和应用.[J].生物医学工程研究2007,(4):368-371
[73]孙思明,周焕英,房彦军等.雌二醇的免疫胶体金试纸法检测.[J].中国公共卫生.2007,(1):126-127。
[74]刘彦慧,罗春丽,吴小候等.细胞角蛋白20单克隆抗体免疫胶体金试纸条的研制.[J].免疫学杂志.2007,(1):174-176
[75]刘先宁,吴洁,朱秀萍等.衣原体单克隆抗体胶体金法测定沙眼衣原体.[J].国际眼科杂志.2007,(6):1759-1760
[76]汪俊云,石峰,杨玥涛等.快速诊断疟疾胶体金免疫层析试条方法的建立与评价.[J].中国寄生虫学与寄生虫病杂志.2007,(5):415-418
[77] Alexander,D.j. Highly pathogenic avian influenza(fowl plague). In OIE Manual of standards for diagnostic tests and vaccines,3rd ed. Office International des Epizooties: Paris, 1996:155-160.
[78] Y.M.Saif主编,苏敬良,高福,索勋主译.禽病学[M].第十一版.中国农业出版社.2005:147-177
[79] Fichtner,G.J. The Pennsylvania/Virginia experience in eradication of avain influenza (H5N2). In B.C.Easterday (ed.). Proceedings of the second international symposium on avian influenza. U.S. Animal Health Association: Richmond, VA, 1987:33-38
[80] Lasley, F.A. Economics of avian influenza:control vs. noncontrol. In Proceedings of the second international symposium on avian influenza,C.W.Beard (ed.).U.S. Animal Health Association: Richmond, VA, 1986:390-399
[81] Stallknecht D E , Shane S M.Host range of avian influenza virus in free-living birds. Veterinary Research Communication,1988,12:125-141
[82]夏咸柱,高玉伟,松涛等.H5N1亚型HPAIV人工感染家猫和小鼠的初步研究CALAS-ICLAS International Symposiumon Laboratory Animal Science
[83] Webster R G,et al.Evolution and ecology of influenza A viruses. Microbiol Reviews, 1991, 56(1): 152-154
[84] Chan P K. Outbreak of avian influenza A (H5N1) virus infection in Hong Kong in 1997. [J].Clin Infect Dis,2002,34(2):58-64.
[85]于康震,陈化兰,唐秀英. 97香港禽流感. [J].中国畜禽传染病,1998,20:187-191
[86]曹伟胜,辛朝安,梁昭平等.2004年亚洲H5N1亚型高致病性禽流感流行特点及启示.[J].中国人兽共患病杂志,2004,20(9):801-804
[87]腾翔雁,郑雪光,宋建德等.2004周边国家高致病性禽流感流行情况概述.[J].中国动物检疫,2004,21(11):44-46
[88]高利波.禽流感病毒分子生物学特性及其诊断进展.[J].云南畜牧兽医, 2003, 2: 10-12
[89]江振友,唐小龙,孙晗笑.禽流感病毒基因组研究.[J].热带医学杂志, 2005, 5(6): 866-868
[90]蔡宝祥等.动物传染病诊断学[M].江苏科学技术出版社,1993:548-551
[91] Krrdel David C. Diagnostic procedures at the state level. Proceedings of the third International Symposium on Avian Influenza. Madison,Wisconsin, USA, 1992: 269-277
[92]郭元吉,杨冬荣,王平等.流感病毒不同快速定型方法敏感性的比较.[J].中华微生物学与免疫学杂志,1984,4(5):289-291
[93]王锡坤,鞠复帮,王立南,等.禽流感琼脂扩散试验抗原制备方法的研究[J].中国畜禽传染病, 1987, 1: 24～26.
[94]赵增连,陈溥言,木祥梅,等.一种敏感的禽流感病毒快速定型双扩散法[J].中国兽医学报,1997,17(3):290-291
[95]邓国华,唐秀英,田国彬等.禽流感病毒重组核蛋白在琼扩诊断中的应用.[J].中国预防兽医学报,2000,22(增刊):25-27
[96] Hurt A C, Barr I G, Komadna N, et al. A novel means of identifying the neuraminidase type of currently circulating human A (H1) influenza viruses [J]. Virus Res, 2004, 103:79-83
[97] Shafer, A. L.,J. B. Katz, and K. A. Eernisse. 1998. Development and validation of a competitive enzymelinked immunosorbent assay for detection of type A influenza antibodies in avian sera. Avian Dis 42:28-34
[98] Zhou E.M., M.Chan, R.A. Heckert, J.Riva, and M. F.Cantin.1998.Evaluation of a competitive ELISA for detection of antibodies against avian nfluenza virus nucleoprotein. Avian Dis 42:142-149
[99]曲站红,邓国华,王桂芹,张立春,田国彬,于康震,陈化兰,. H5亚型高致病性禽流感病毒抗原捕捉ELISA诊断方法的建立[J].中国预防兽医学报,2008,(1):68-71
[100]韩庆功,崔艳红,张智勇. ELISA技术在禽流感诊断研究中的应用[J].生物技术通报,2008,(6):73-77
[101]李海燕,辛晓光,田国斌,等禽流感抗体斑点- ELISA诊断技术的研究.[J].中国预防兽医学报,1999,21(5):321-325
[102]李海燕,于康震,辛晓光,等.禽流感病毒重组核蛋白ELISA诊断技术的研究[J]中国预防兽医学报,2000,22(3):182-185
[103]王传彬,田新生,曹振等. H5亚型禽流感病毒单抗—生物素捕获ELISA的建立. [J].中国实验动物学报,2004,12(4):204-207
[104] Munch M, Nielsen LP, Handberg KJ, et al. Detection and subtyping (H5andH7) of avian type a influenza virus by reverse transcription-PCR and PCR-ELISA. ArchVirol,2001,146:87-97
[105]Dybkaer K, Munch M, Handberg KJ, et al. Application and evaluation of RT-PCR-ELISA for the nucleoprotein and RT-PCR for detection of low pathogenic H5 and H7 subtypes of avian influenza virus. J Vet Diagn Invest, 2004, 16: 51-56.
[106]庞耀珊,谢芝勋,邓显文等.多重反转录聚合酶链反应检测H5亚型禽流感病毒方法的建立.[J].中国人兽共患病杂志, 2005,21(9):762-764
[107]吕长荣,乔海莲,杨增岐,陈杰,顾节清,李晓成.一步法多重RT-PCR禽流感病毒分型诊断法的建立与应用[J].西北农林科技大学学报(自然科学版), 2007, (4):63-67
[108] Collins R A, KoL S, SoK L, et al. Detection of highly pathogenic and low pathogenic avian influenza subtype H5 (Eurasian lineage) using NASBA. J Virol Methods, 2002,103:213-225
[109] Collins R A, KoL S., FungK Y, et al. Rapid and sensitive detection of avian influenza virus subtype H7 using NASBA. [J].Biochem Biophy Res Commun, 2003, 300: 507-515
[110] Shan S, KoL S, Collins R A, et al. Comparison of nucleic acid-based detection of avian influenza H5N1 with virus isolation. [J]. Biochem Biophy Res Commun, 2003, 302: 377-383
[111] Collins R A, KoL S, SoK L, et al. A NASBA method to detect high- and low- pathogenicity H5 avian influenza viruses. [J].Avian Dis,2003,47:1069-1074
[112] Lau L T, Bank J, Aherne R, et al. Nucleic acid sequence-based amplification methods to detect avian influenza virus. [J].Biochem Biophy Res Commun, 2004, 313: 336-342
[113]王楠,邹明强,汪明,李锦丰,薛强,张帆,.禽流感病毒流式微球量子点探针免疫诊断新方法[J].分析测试学报,2009,(7):764-768
[114]石霖,王秀荣,杨忠苹,陶启蒙,王煜,武嘉男,蔡冬冬,包红梅,陈化兰,.禽流感、新城疫可目视化诊断蛋白芯片的制备及初步应用[J].中国预防兽医学报,2009,(1):60-64
[115]崔仑标,唐震,曾晓燕,史智扬,焦永军,葛以跃,马明,单云峰,吴涛,陈银,李显,汪华,.液相芯片技术在诊断江苏省首例人禽流感病例中的应用[J].现代预防医学,2008,(23):4666-4667
[116]张鹤晓,赖平安,刘环,等.荧光RT-PCR检测活禽和禽组织中禽流感病毒的研究.[J].检验检疫科学,2004,14(1):1-5
[117] World Health Organization. Anti-tuberculosis drug resistance in the world. Report No.2. Prevalence and trends. Geneva:WHO,2000
[118]肖东楼.中国结核病防治工作进展与成就.[J].中国防痨杂志.2008,30(6):491-493
[119]全国结核病流行病学抽样调查技术指导组.第四次全国结核病流行病学抽样调查报告.[J].中华结核和呼吸杂志.2002,25(1):3-7
[120]程渝,李茂全.结核分枝杆菌的实验室诊断进展.[J].国外医学临床生物化学与检验学分册.2002,23(6):342-343
[121]周丽荣,罗永爱.结核病血清学诊断的新进展.[J].中国实用内科杂志.2005,3(25):296-271
[122]姜永忠.结核病的特异性免疫诊断.[J].国外医学预防、诊断、治疗用生物品分册.2001, 24 (3): 116-120.
[123] Cole ST, Brosch R, Parkhill J, et al. Deciphering the biology of mycobacterium tuberculosis from the complete genome sequence .Nature.1998, 393(6685):537-544
[124] Alito A, Me Nair J, Girvin RM. Identification of Mycobacterium bovis antigens by analysis of bovin T-cell responses after infection with a virulent strain, Braz.[J].Med Bio Res. 2003, 36: 1523-1531.
[125] Laurens A. H.van Pinxteren, Pernille Ravn, Elea Marie Agger, et al. Diagnosis of Tuberculosis based on the Two Specific Antigens ESAT-6 and CFP10 [J].Clin Diagn Lab Immunol, 2000, 7: 155-160
[126] HarboeM, OettingerT, WikerHG, et al. Evidence for occurence of the ESAT-6 protein in mycobacterium tuberculosis and virulent Mycobacterium bovis and for its absence in Mycobacterium bovis BCG [J]. Infect Immun. 1996, 64 (1):16-22
[127] Mahairas G G, Sabo P J, hickey M J, et al. Molecular analysis of genetic differences between Mycobacterium bovis BCG and virulent M. bovis [J]. Bacteriol ,1996 ,178 :1274～1282
[128] Sandra M Arend , Peter Andersen , Krista E. van Meijgaarden , et al. Detection of active tuberculosis infection by T-cell response to early-secreted antigenic target 62kDa protein and culture filtrate protein 10 [J] . The Journal of Infectious Diseases , 2000 , 181 :1850～1854
[129]李娟,吴雪琼,张俊仙等.结核分枝杆菌CFP10-ESAT6融合蛋白在大肠杆菌中的高效表达.[J].中国防痨杂志.2004,26(4):204-208
[130] Dillon DC, Alderson MR , Day CH , et al . Molecular and immunological characterization of Mycobacterium tuberculosis CFP– 10, An Immunodiagnostic Antigen Missing in Mycobacterium bovis BCG .[J].Clin Micro biol , 2000 , 38 (9) :3285
[131] Wiliins E G, Ivanyi J. Potential value of serology for diagnosis of extrapulmonary tuberculosis [J ].Medicine Science ,1990 ,336 :641～644
[132] Zhou A T, Ma WL ,Zhang P Y, et al . Detection of pulmonary and extrapulmonary tuberculosis patients with the 38 - kilodalton antigen from Mycobacterium tuberculosis in a rapid membrane based assay[J].Clinic Diagnostic Laboratory Immunology ,1996 ,3 :337～341
[133] Nakamura RM, Velmonte MA, Kawajiri K, et al.MPB64 mycobacterial antigen: a new skin-test reagent through patch method for rapid diagnosis of active tuberculosis. Int [J]. Tuberc. Lung Dis, 1998, 2(7):541
[134]李香兰,吴雪琼,张广宇等.结核分支杆菌分泌蛋白Mtb81在大肠杆菌中的高效表达. [J].河北医科大学学报.2005,26(1):25-28
[135] Raymond L , Houghton M, Davin C , et al . Use of multiepitope polyproteins in serodignosis of active tuberculosis[J ] . Clin Dinga Lab Immunol , 2002 , 9 (4) : 883-891.
[136] Lim JH, Park JK, et al. Purification and immunoreactivity of three components from the 30/32-kilodalton antigen 85 complex in Mycobacterium tuberculosis.[J]. Infect Immum, 1999, 67 (11): 6187
[137] Wiker H G, Harboe M. The antigen 85 complex: A major secretion product of Mycobacterium tuberculosis. [J]. Microbiol Rev, 1992, 56:648-661
[138]范雄林,徐志凯,李元等.结核分枝杆菌Ag85B分泌蛋白基因疫苗的构建和免疫原性的研究. [J].中华结核和呼吸杂志,2001,24:548-550
[139] Xinmiao Fu, Zengyi Chang. Temperature-dependent subunit exchange and chaperone -like activities of Hsp16. 3, a small heatshock protein from Mycobacterium tuberculosis [J]. Biochemical and Biophysical Res Commun, 2004, 316 (4) : 291.
[140] Agrewala JN, Wilkinson RJ. Differential regulation of Th1 and Th2 cell by p91-110 and p21-40 peptides of the 16-kD alpha-crystallin antigen of Mycobacterium tuberculosis. [J]. Clin Exp Immunol.1998,114 (3) : 392-397.
[141]徐磊,李慧云,马立恒.四种结核分枝杆菌特异性抗原的克隆表达与血清鉴定.[J].中华结核和呼吸杂志. 2004,27(3):188-190
[142] Lyashchenko K,Colangeli R , Houde M , et al. Heterogeneous antibody responses in tuberculosis. Infect Immun ,1998 ,66 (8) :3936-3940
[143]吴雪琼,张俊仙,夏湘萱等.结核分支杆菌MPT63抗原的表达及其血清学诊断价值的研究.[J].中国现代医学杂志.2001,11(4):8-11
[144] Li H, U lstrup JC, Tonassen TO , et al. Evidence for the absence of the M PB64 gene in some substrains of M ycobacterium bovis BCG . Infect Immun, 1993, 61: 1730-1734.
[145]吴雪琼,张俊仙,李洪敏等.结核分枝杆菌MPT64蛋白的表达,纯化及初步应用[J].中国防痨杂志,2001,23(2):85-88
[146]熊志红,庄玉辉,张晓刚等.结核分支杆菌MPT64基因的克隆及其在大肠肝菌中的表达、纯化和诊断运用.[J].中国现代医学杂志.2004,14(21):48-51
[147] Okud Y, Maekura R, Hirotani A, et al. Rapid serodiagnosis of active pulmonary Mycobacterium tuberculosis by analysis of results from multiple antigen-specific tests.[J].Clin Microbiol, 2004,42(3):1136
[148]崔君兆.弓形虫病研究九十年[J].实用寄生虫病杂志,2000,8(2).
[149]于恩庶主编.弓形虫病学(第1版)[M].福州,福建科学技术出版社,1992.
[150] Savva, D., Morris, J. C., Johnson, J. D., and Hillman. Polymerase chain reaction for detection of Toxoplasma gondii. [J]. Med. Microbiol,1990,32:25–31.
[151]程玉芝,韩广东.弓形虫病的临床诊断. [J].中国寄生虫病防治杂志,1990,3(2):157
[152]吕元聪,崔君兆.我国部分地区弓形虫病流行病学监测.[J].实用寄生虫病杂志,1994,2(3):19-22
[153]林敏,吴少庭,高世同,等.深圳人群弓形虫感染的血清流行病学调查研究[J].寄生虫与医学昆虫学报,2000,7(3):135-139
[154]赵玉强,郑雅梅,闫歌,等.山东地区饲养动物与接触动物职业者弓形虫感染调查.[J].中国热带医学.2001,1(1):69
[155]王崇功,周永华.弓形虫感染对优生优育危害性及其控制的研究[J].中国血吸虫病防治杂志,1998,10(6):362-365
[156]刘爱芹,张唯哲,王光岳等.不明原因智力低下学生弓形虫感染的调查.[J].哈尔滨医科大学学报,1998,12(6):409
[157]王海琦,杜青,倪黎等.孕妇感染弓形虫影响胎儿发育跟踪研究[J].中国人兽共患病杂志,1997,13(2):48-50
[158]邹孟红,周雅,徐惠颖等.未婚妊娠妇女的心理行为和社会因素调查[J].中国心理卫生杂志.1997,11(1):15-17
[159]杨迎工,韩胜凯,高虹等.不孕症与弓形虫感染的检测意义[J].实用男科杂志. 1995,1(1):49-50.
[160]马济宏,郭汉琴,蔡云平.不孕症患者弓形虫抗体检测与分析[J].中国人兽共患病杂志.1996,12(5):封3.
[161]周永华,陆永娟,王瑞兵,等.苏州市农村不孕症夫妇弓形虫感染调查[J].中华男科学,2002,8(5):350-352
[162]黄自平,黄征杰,郭淮,等.140例男性不育病人精浆抗弓形虫抗体检测结果分析[J].中国计划生育学杂志,1998,4:160-161
[163] Marrinez AJ,Sell M,Mitrovices T,et al. The neuropathology and epidemiology of AIDS. A review of 200 cases. [J].Pathol Res Pract,1995,191:427-443
[164] Carey RM,Kimball AC,Armstring D,et al.Toxoplasmosis clinical experience in a cancer hospital . [J].Am J Med,1973,54:30-38
[165]彭丽娟,鲍杨漪,许兰芳等.恶性肿瘤患者弓形虫抗体的调查[J].中国寄生虫学与寄生虫病杂志.1996,14:25
[166]王宝林,潘孝彰,尹有宽等.免疫缺陷性疾病患者弓形虫抗体的调查[J].中国寄生虫学与寄生虫病杂志.2000,18(4):224-226
[167]刘爱芹,李雅杰.不同临床类型的恶性肿瘤患者弓形虫感染调查[J].中国人兽共患病杂志.1998,14(6):73-74
[168]卢致民,张振明,牛发良,等.肿瘤患者弓形虫感染调查[J].中国寄生虫病防治杂志,2003,16(4):203,207
[169]卢慎,郭琴荣.弓形虫病的实验室诊断.[J].医师进修杂志,1987,10(7):28
[170]李允鹤主编.寄生虫病免疫学及免疫诊断(第1版)[M].江苏,江苏科学技术出版社.1991.
[171]李观娣,杜重波,陈义民,等.弓形虫在培养细胞中生物学特性的观察.[J].中国兽医科技,1986,4:10
[172]魏梅雄,张述义,郑思民,等.弓形虫病直接凝集试验的研究.[J].中国人兽共患病杂志,1996,12(1):19
[173]杜惠芬等.弓形虫金标检测卡研制,未发表资料.
[174]陈晓光,刘国章,王洵章,等.含弓形虫P30基因插入昆虫杆状病毒的研究.[J].中国寄生虫学与寄生虫病杂志,1996,14(2):270-273
[175]陈晓光,刘国章,唐银明,等.弓形虫巢式PCR体系的建立及其对人、鼠弓形虫病的检测.[J].中国寄生虫病防治杂志,1996,9(1):53-54
[176]孙丽萍,马静.人H5N1病毒感染及其防治现状[J]..中国预防医学杂志,2007, 8(6): 764-769.
[177] Capua I, Alexander DJ. Human health implications of avian influenza viruses and paramyxoviruses [J]. Eur J Clin Microbiol Infect Dis, 2004, 23:1-6.
[178]廖华乐.禽流感病毒与人类健康[J].中国食品卫生杂志,,1998,10(5):
[179]马鸣潇,金宁一,王振国,等.检测禽流感病毒RT-PCR一步法的建立及H5、H9亚型的鉴定[J].中国生物制品学杂志,2005, 18(5): 415-417.
[180]魏泉德,谭爱军.禽流感病毒实验检测研究进展[J].微生物学通报,2007, 34(5): 986-990.
[181]刘维全,刘海鹏,江禹,等.禽流感病毒、新城疫病毒、猪瘟病毒和口蹄疫病毒多联RT-PCR诊断技术的建立[J].农业生物技术学报,2005, 13(1): 92-95.
[182]韩剑锋,宁宜宝,宋立,等.实时荧光PCR技术快速鉴别检测H5、H9、H7亚型禽流感灭活疫苗的研究[J].生物工程学报,2007, 23(5): 953-957.
[183]韩刚毅.胶体金标记抗体技术[J].生物化学与生物物理进展,1989, 16(1): 31-35.
[184] Houiman RE, Raymond R, Renton N, et al. The diagnosis of toxoplasmosis using IgG avidity[J]. Epidemiol Infect, 1994, 112: 399-408.
[185]邵军军.胶体金免疫层析试纸条在病原体检测及医学诊断中的应用[J].实用诊断与治疗杂志,2008, 22(1): 39-40.
[186] Kumar H, Malhotra D, Goswami S, et al. How far have we reached in Tuberculosis vaccine development[J]. Crit rev microbiol, 2003, 29 (4): 297-312.
[187] Anderson AB, Hansen EB. Structure and mapping of antigenic domains of protein antigen b, a 38 kb molecular weight protein of Mycobacterium tuberculosis[J].. Infect Immun, 1989, 57: 2481-2488.
[188] Ivanyi J, BothamLey GH, Jackett PS, et al. Immuno-diagnostic assays for tuberculosis and leprosy[J]. Br Med Bull, 1988, 44: 635-649.
[189] Kingston AE, Salgame PR, Mitchisoa A, et al. Immunological activity of a 14 kilodalton recombinant protein of Mycobacterium tuberculosis H37RV[J]. Infect Immun, 1987, 55: 3149-3154.
[190] Lee BY, Hefta SA, Brennan PJ. Characterization of the major membrane protein of virulent Mycobacterium tuberculosis[J]. Infect Immun, 1992, 60: 2066-2074.
[191] Fu X, Chang Z. Temperature-dependent subunit exchange and chaperone-like activities ofHsp16.3, a small heatshock protein from Mycobacterium tuberculosis[J]. Biochem Biophys Res Commun, 2004, 316 (2): 291-299.
[192] Verstijnen CP, Scokmingh R, Kuijper S, et al. Rapid identification of cultural Mycobacterium tuberculosis with a panel of monoclonal antibodies in Western blot and immunofluorescence [J]. Res Microbiol, 1989, 140: 653-666.
[193] Hu Y, Philip D. Protein synthesis is shutdown in dormat Mycobacterium tuberculosis and is reversed by oxygen or heat shock[J]. FEMS Microbiol Lett, 1998, 158 (1): 139.
[194] BothamLey GH, Rudd R, Festenstein F, et al. Clinical value of the measurement of Mycobacterium tuberculosis specific antibody in pulmonary tuberculosis[J]. Thorax, 1992, 47(4): 270
[195]熊志红,庄玉辉,张晓刚,等.结核分枝杆菌16000抗原基因在大肠杆菌中的高效表达及其产物纯化[J].中华结核和呼吸杂志,1999, 22(11): 645-647.
[196]杨军兰,杨侠.结核分枝杆菌16KD蛋白的基因克隆及表达与纯化[J].实用医技杂志,2008, 15(29): 4001-4003.
[197] Yong D, Kent L. Immunoligic activty of a 38-kilodaton protein purified from Mycobacterium tuberculosis[J]. Infect Immun, 1986, 54(1): 177-183.
[198] Andersen AB, Ljungqvist L, Olsen M, et al. Evidence that protein anigen of mycobacterium tuberculosis is invloved in phosphste metabolism[J]. Gen microbiol, 1990, 136: 477-480.
[199] Chang Z, Abha C, Raju L, et al. The immunodominant 38 kDa liproprotein antigen of Mycobacterium tuberculosis is a phosphate binding protein. J Biol Chem, 1994, 269: 1956-1958.
[200] Kadival GV, Chaparas SD, Hussong D. Characterization of serologic and cell mediated reactivity of a 38 kDa antigen isolated from Mycobacterium tuberculosis[J]. J Immun, 1987, 139: 2447-2451.
[201] Singh M, Andersen AB, Mclarthy JE, et al. The Mycobacterium tuberculosis 38 kDa antigen: overexpression in Escherichia coli, purification and characterization[J]. Gene, 1992, 117: 53-60.
[202] Souza CD, Kadival GV, Samuel AM. Use of the recombinant 38 kDa antigen of Mycobacterium tuberculosis as an immunogen for specific antisera production[J]. Microbiol Immun, 1994, 38: 797-800.
[203] Haslov K, Andersen AB, Ljungqvist L, et al. Comparison of the immunological activity of five defined antigens from Mycobacterium tuberculosis in seven inbred guinea pig strains, the 38 kDa antigen is immunodominant[J]. Scand J Immun, 1990, 31: 503-514.
[204] Wilkinson RJ, Haslov K, Rappuoli R, et al. Evaluation of the recombinant 38 kilodalton antigen of Mycobacterium tuberculosis as a potential immunodiagnostic reagent[J]. J Clin Microbiol, 1997, 35: 553-557.
[205]何秀云,庄玉辉,张晓刚,等.结核分枝杆菌38000蛋白质抗原在大肠杆菌中高效表达[J].中华结核和呼吸杂志,1999, 22 (3): 138-141.
[206]吴燕,朱中元,王海波.结核分枝杆菌38KD蛋白基因克隆及在大肠杆菌中的表达[J].中国热带医学,2006, 5(9):1786-1789.
[207]苏明权,岳乔红,周萍等,结核分枝杆菌重组蛋白的制备及抗原性分析[J].第四军医大学学报,2006,27(11): 987-990.
[208]周丽蓉,徐敬华,罗永爱,等.结核分枝杆菌38 ku蛋白的制备及抗原性研究[J].中国实用内科杂志,2007,27(24): 1955-1957.
[209]刘贤杰,朱中元,陈奎霖.重组结核分支杆菌38KDa蛋白抗原制备及其血清学诊断价值[J].中国热带医学,2008, 8(8): 1275-1277.
[210] He XY, Luo YA, Zhang XG, et al. Clinical evaluation of the recombinant 38 kDa protein of Mycobacterium tuberculosis[J]. Scand J Infect Dis, 2008, 40(2): 121-126.
[211]朱中元,王海波,刘海国,等.抗结核分支杆菌多种抗原的抗体检测蛋白芯片研究[J].中国热带医学,2004, 4(6): 907-910.
[212]张小刚,庄玉辉,何秀云,等.重组结核分支杆菌38kD和16kD蛋白用于结核病血清学诊断的价值[J].中国防痨杂志,2001, 23 (5): 183.
[213]汪明.兽医寄生虫学[M].第三版,北京:中国农业出版社, 2005.
[214]刘德纯,林清森,Seshan V.获得性免疫缺陷综合症(AIDS)合并播散性弓形虫病尸检的病理学研究[J].中华病理学杂志, 1994, 23(3): 166-169.
[215] Tsuda Y, Sakoda Y, Sakabe S, et al. Development of an immunochromatographic kit for rapid diagnosis of h5 avian influenza virus infection[J]. Micrology and immunology, 2007, 51: 903-907
[2] Le QM, Kiso M, Someya K, et al. Avian flu: isolation of drug-resistant H5N1 virus. [J]. Nature 2005, 437(7062):1108
[3] Mebride MT,Gammon S,Pitesky M,et al. Multiplexed liquid arrays for simultaneous detection of simulants of biological warfare agents. [J]. Anal Chem,2003,75(8):1924-1930
[4]李卫华.食品微生物快速检测方法进况.现代商务科技.[J].1998, 3(8) :53-56
[5]商检行业标准.SN0133-94.出口食品中大肠杆菌(葡萄糖酶荧光)检验方法.1994
[6]甄宏太,杨素贤,刘中学,等.应用MUCAP试剂快速检测沙门氏菌.[J].微生物学报.1996 , 36 (1) : 586-592.
[7]陆茂林,蔡建荣,赵晓联,等.标记免疫分析技术在食品分析中的应用.[J].中国卫生检验杂志. [J].2002,12(2):60-62
[8]龙军,陈清,俞守义.双抗体夹心ELISA法检测葡萄球菌B型肠毒素.[J].中国公共卫生.2005,21(2):154-155
[9]涂少华,叶元康,沈昭在.双抗体夹心ELISA检测肺炎支原体抗原方法的建立.[J].检验医学, 2005 , 20 (3) :214-216.
[10] Gehring AG, Irwin PL, Reed SA, et al. Enzyme-linked immunomagnetic chemiluminescent detection of E.coli O157:H7 .[J].Immunol Meth, 2004, 293(12): 97-106
[11]吕治林.两种成分联合的直接荧光抗体技术对细菌性炭疽的快速诊断.[J].国外医学.流行病学.传染病学分册.2003 , 1(30) :58.
[12] Mata AI, Gibello A, Casamayor A, et al. Multiplex PCR assay for detection of bacterial pathogens with associated warm-water streptococcosis in fish.[J].Appl Environ Microbiol. 2004, 70 (5) :3183-3187.
[13] Glukhov AI, Gordeev SA, Al’tshuler ML, et al. Study of the effectiveness of the method of the nest polymerase chain reaction and the field of its use in hepatitis A diagnosis.[J]. Zh Mikrobiol Epidemiol Immunobiol 2004 , 6: 88-91.
[14] Boutaga K, van Winkelhoff AJ, and enbroucke-Grauls CM ,et al . Comparison of real-time PCR and culture for detection of porphyromonas gingivalis in subgingival plaque samples. [J]. Clin Microbiol , 2003 ,41 (11) : 4950-4954.
[15]Rehman FN, Audeh M, Abrams ES, et al. Immobilization of acrylamide-modified oligonucleotides by co-polymerization .[J].Nucleic Acids Res. 1999, 27(2):649-655.
[16] Sano T, Smith CL, and Cantor CR. Immuno-PCR: very sensitive antigen detection by means of specific antibody-DNA conjugates.[J].Science.1992, 258(5079): 120-122.
[17] Niemeyer CM , Adler M , Wacker R. Immuno-PCR: high sensitivity detection of proteins by nucleic acid amplification.[J].Trends Biotechnol , 2005 ,23 (4) : 208-216
[18]翟新验,卢胜明,刘钧.实时TaqMan-PCR技术在兽医学上的应用.[J].实验动物科学与管理,2002,6(2):19-22
[19] Munch M, Nielsen LP, Handberg KJ , et al . Detection and subtyping (H5 and H7) of avian type A influenza virus by reverse transcription-PCR and PCR ELISA .[J]. Arch Virol. 2001,146 (1) : 87-97.
[20]林万明主编,临床基因探针诊断实验技术.[M].上海科学技术出版社, 1993
[21] Burton W.B, Lucille M.P. A simple RNA probe system for analysis of Listeriamonocytogenes polymerase chain reaction products .[J]. Applied and Environmental Microbiology. 1993, 59 (9):2795-2800
[22] Amann RI, Krumholz L, Stahl DA. Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in micro- biology .[J].Bacteriol.1990, 172:762-770.
[23]Mahmoud KK,Leduc LG,Ferroni GD. Detection of Acidithiobacillus ferrooxidans in acid mine drainage environments using fluorescent in situ hybridization (FISH).[J]. Microbiol Methods. 2005,61 (1):33-45.
[24]葛晶,殷涌光,王凯.使用SPR生物传感器快速检测大肠杆菌E.coli O157:H7.[J].吉林大学学报(工学版). 2005, 35(2): 214-218.
[25] Masarova J, Dey ES, and Danielsson B. Optical lectin based biosensor as tool for bacteria identification.[J]. Clin Microbiol. 2004, 53: 23-27
[26]邱景富,王升启.生物芯片技术及其在病原微生物检测方面的进展.[J].中国微生态学杂志.2003,15(2):124
[27] Anthony RM ,Brown TJ, French GL.Rapid diognosis of bacteria by universal amp lification of 23S ribosomal DNA followed by hybridization to an oligonucleo- tide array. [J].Clin Microbiol, 2000, 38(20) : 781-788
[28] Jack S, Dougles RC, F red JB, et al. Direct detection of 16S RNA in soil extracts by using oligonulrotide microarrays .[J].App Env Microbiol, 2001, 67(10): 4708-4716
[29] Zhang Llin-qi, YuWen-jie, He Tian,et al. Contribution of human alpha-defensin 1, 2, and 3 to the anti-HIV-1 activity of CD8 antiviral factor.[J]. Science. 2002, 298: 995-1000.
[30] KangXi-xiong, XuYang, Wu Xiao-y,ietal. Establish of the proteomic fingerprints for potential application to early diagnosis of severe acute respiratory syndrome (SARS).[J]. Clin Chem. 2005, 51: 56-64
[31] R.G. McClelland, Detection of Salmonella typhimurium in dairy products with flow cytometry and monoclonal antibodies.[J]. Appl Environ Microbiol.1994, 60(12): 4255-4262
[32] Bio merieux公司产品介绍
[33]刘敏,汤一苇. IbisT5000:一种新型病原微生物检测仪.[J].微生物与感染. 2008,3(4):254-255
[34] Mcdade RL, Spain MD. Rapid, economical testing in the clinical laboratiory: a new flow cytometry-based multiplex system .[J]. Clin Irnmunol Newsletter. 1997, 17(10): 154-158
[35]胡瑞,赵金银,陈德坤等.应用xMAP液态芯片多重快速检测四种病原微生物的研究.[J].现代生物医学进展. 2008,8(12):2232-2235
[36]陆学东,周一平,杨来智等.多种呼吸道病原微生物快速筛查技术的建立.[J].中华医院感染学杂志.2008,18(1):140-143
[37]鲁卫平,顾大勇,王华等.基于纳米金的显色基因芯片快速检测病原微生物.[J].生物技术通报.2008,3:95-98
[38]张如胜.LAMP技术在病原微生物检测中的应用.[J].华南预防医学.2007,33(5): 45-49
[39] Romano El, et al.[M].Historical aspects. Academic Press. Amsterdam New. Oxford, 1984.
[40] Horisberger M, Rosset. Colloidal gold, a useful marker for transmission and scanning electron microscopy .[J].Histochem .Cytochem. 1977,25(4):295-305.
[41] Stathis. E.C .[M]. Fabrikanos A. Chem. Ind.1958,27:860.
[42] Hirisberger M, Tacchini-Vonlanthen M. Ultrastructural localization of Kunitz inhibitor on thin sections of Glycine max (soybean) cv. Maple Arrow by the gold method.[J].Hisiochemistry.1983,77(1):37-50.
[43] Fren G. Controlled Nucleation for the Regulation of the Particle Size in Monodisperse Gold Suspensions.[J].Nature, Phys Sci .1973,241:20.
[44] Horisberger M. Evaluation of colloidal gold as a cytochemical marker for transmission and scanning electron microscopy.[J]. Biol Cell. 36:253-259.
[45] Baigent C. L. and G. Muller. A colloidal gold prepared with ultrasonics.[J]. Experientia. 1980,36:472-473.
[46] Tschopp J. Podack ER, Müller-Eberhard HJ. Ultrastructure of the membrane attack complex of complement: Detection of the tetramolecular C9-polymerizing complex C5b-8.[J]. Proc Natl Acad Sci.USA,1982,79(23):7474-7478.
[47] Slot JW. A method to prepare isodisperse colloidal gols sols in size range 3-17nm . [J]. Ultramicroscopy.1985,15:383.
[48] Slot JW,Geuze HJ. Sizing of protein A-colloidal gold probes for immunoelectron microscopy .[J].Cell Biol.1981,90:533-536.
[49] De Mey J, Moeremans M, Geuens G, et al. High resolution light and electron microscopic localization of tubulin with the IGS (immuno gold staining) method .[J]. Cell Biol. 1981 ,5(9):889–899
[50] Faulk WP, Taylar G.M. An immunocolloidal method for the electron microscope. [J].Immunochemistry. 1971, 8:1081-1084
[51] Hodges GM, Smolira MA, Livingston D. Scanning Electron Microscope Immunocytochemistry in Practice: Immunolabelling For Electron Microscopy. [M]. Elsevier Science Publishers BV, Amsterdam, 189-233.
[52]Slot JW. et al.[M]. Immunolabelling For Electron Microscopy Press. New York, 1984.129-142
[53] Singer S.J, Tokuyasu K. T, Gilbert-A. et al.Immunoelectron Microscopy and the Molecular Ultanstructure of Cells .[J].Electron Microsc.1987,36(3);63-71
[54] Feldherr C.M, and Marshall J. The use of colloidal gold for studies of intracellular exchanges in the ameba Chaos chaos.[J]. Cell Biol. 1962,12:640–645
[55] Valkirs GE,Barton R. lmmuno concentration--a new format for solid-phase immunoassays.[J]. Clin Chem,1985,31:1427-1431.
[56] Spielberg F, Kabeya CM, Ryder RW, et al. Field testing and comparative cvaluation of rapid, visually read screening assays for antibody to human immunodeficiency virus. .[J]. Lancet.1989,1:580-584
[57]沈丽英,干小仙,丁建祖等.检测幽门螺杆菌抗体的金标免疫渗滤法的建立及应用.[J].浙江实用医学,1997,(4):16-17
[58]陈国庆,古洁若,陆才生等.金标免疫渗滤法检测抗角质蛋白抗体在类风湿性关节炎诊断中的意义.[J].广东医学.2003,(10):1093-1094
[59]陈国庆,古洁若,黄建林等. A组溶血性链球菌膜抗原吸附心肌抗体金标免疫渗滤法的建立.[J].现代检验医学杂志,2003,(5):7-8
[60]沈丽英,干小仙,丁建祖等.金标免疫渗滤法(DIGFA)快速检测血吸虫循环抗原的研究[J].中国人兽共患病杂志.2000,(1):12-14。
[61]施晓华,汤益,王越等.成虫三氯醋酸抽提抗原金标免疫渗滤法诊断日本血吸虫病及考核疗效的研究.[J].中国血吸虫病防治杂志.2006,(2):116-118
[62]顾炜峰,王越,干小仙.金标免疫渗滤法检测猫狗血清中抗肺吸虫IgG..[J].医学动物防制.2008,(2):119-120
[63] Beggs.M, Novotny.M, Sampedron.S,et al.A selfperforming chromatographic immunoassay for the qualitive detemination of human chorionic gonadtrophin (HCG) in urine and serum. [J].Clinchem, 1990, 36:1084-1085.
[64]周继文,戎广亚,扬守纯等,胶体金免疫层析法检测乙型肝炎病毒表面抗原[J].中华医学检验杂志.1998,21(1):30-32.
[65]吴守丽,严延生,李世清等.重组汉坦病毒核衣壳蛋白抗原用于胶体金标记免疫层析法检测肾综合征出血热抗体.[J].中华微生物学和免疫学杂志.2007,27(2): 192-196
[66]陈军政.免疫胶体金法快速检测轮状病毒腹泻的结果分析.[J].现代预防医学.2007,(9):1763-1764
[67]胡凤娟.胶体金法筛查丙型肝炎抗体在街头献血车中的应用.[J].检验医学,2007,(4):516
[68]蒋韬,梁仲,陈涓等.检测AsiaⅠ型口蹄疫病毒的胶体金免疫层析法的建立及应用.[J].细胞与分子免疫学杂志.2007,(11):1021-1024
[69]程庆,刘志伟,郑定容等.胶体金法在HIV抗体检测中的应用.[J].实用医技杂志. 2008,(17):2207-2208
[70]何艳玲,林松,王陆迪等.体金免疫层析法检测水产品中O_1群霍乱弧菌方法的建立与优化.[J].中国国境卫生检疫杂志.2007,(1):52-55
[71]刁琳琪,王伟,徐建国等.胶体金标记免疫层析法检测大肠杆菌O157.[J].疾病监测.2007.(7):438-440
[72]赵晶,姜海燕,杜民等.毒品胶体金试剂快速测试仪的研制和应用.[J].生物医学工程研究2007,(4):368-371
[73]孙思明,周焕英,房彦军等.雌二醇的免疫胶体金试纸法检测.[J].中国公共卫生.2007,(1):126-127。
[74]刘彦慧,罗春丽,吴小候等.细胞角蛋白20单克隆抗体免疫胶体金试纸条的研制.[J].免疫学杂志.2007,(1):174-176
[75]刘先宁,吴洁,朱秀萍等.衣原体单克隆抗体胶体金法测定沙眼衣原体.[J].国际眼科杂志.2007,(6):1759-1760
[76]汪俊云,石峰,杨玥涛等.快速诊断疟疾胶体金免疫层析试条方法的建立与评价.[J].中国寄生虫学与寄生虫病杂志.2007,(5):415-418
[77] Alexander,D.j. Highly pathogenic avian influenza(fowl plague). In OIE Manual of standards for diagnostic tests and vaccines,3rd ed. Office International des Epizooties: Paris, 1996:155-160.
[78] Y.M.Saif主编,苏敬良,高福,索勋主译.禽病学[M].第十一版.中国农业出版社.2005:147-177
[79] Fichtner,G.J. The Pennsylvania/Virginia experience in eradication of avain influenza (H5N2). In B.C.Easterday (ed.). Proceedings of the second international symposium on avian influenza. U.S. Animal Health Association: Richmond, VA, 1987:33-38
[80] Lasley, F.A. Economics of avian influenza:control vs. noncontrol. In Proceedings of the second international symposium on avian influenza,C.W.Beard (ed.).U.S. Animal Health Association: Richmond, VA, 1986:390-399
[81] Stallknecht D E , Shane S M.Host range of avian influenza virus in free-living birds. Veterinary Research Communication,1988,12:125-141
[82]夏咸柱,高玉伟,松涛等.H5N1亚型HPAIV人工感染家猫和小鼠的初步研究CALAS-ICLAS International Symposiumon Laboratory Animal Science
[83] Webster R G,et al.Evolution and ecology of influenza A viruses. Microbiol Reviews, 1991, 56(1): 152-154
[84] Chan P K. Outbreak of avian influenza A (H5N1) virus infection in Hong Kong in 1997. [J].Clin Infect Dis,2002,34(2):58-64.
[85]于康震,陈化兰,唐秀英. 97香港禽流感. [J].中国畜禽传染病,1998,20:187-191
[86]曹伟胜,辛朝安,梁昭平等.2004年亚洲H5N1亚型高致病性禽流感流行特点及启示.[J].中国人兽共患病杂志,2004,20(9):801-804
[87]腾翔雁,郑雪光,宋建德等.2004周边国家高致病性禽流感流行情况概述.[J].中国动物检疫,2004,21(11):44-46
[88]高利波.禽流感病毒分子生物学特性及其诊断进展.[J].云南畜牧兽医, 2003, 2: 10-12
[89]江振友,唐小龙,孙晗笑.禽流感病毒基因组研究.[J].热带医学杂志, 2005, 5(6): 866-868
[90]蔡宝祥等.动物传染病诊断学[M].江苏科学技术出版社,1993:548-551
[91] Krrdel David C. Diagnostic procedures at the state level. Proceedings of the third International Symposium on Avian Influenza. Madison,Wisconsin, USA, 1992: 269-277
[92]郭元吉,杨冬荣,王平等.流感病毒不同快速定型方法敏感性的比较.[J].中华微生物学与免疫学杂志,1984,4(5):289-291
[93]王锡坤,鞠复帮,王立南,等.禽流感琼脂扩散试验抗原制备方法的研究[J].中国畜禽传染病, 1987, 1: 24～26.
[94]赵增连,陈溥言,木祥梅,等.一种敏感的禽流感病毒快速定型双扩散法[J].中国兽医学报,1997,17(3):290-291
[95]邓国华,唐秀英,田国彬等.禽流感病毒重组核蛋白在琼扩诊断中的应用.[J].中国预防兽医学报,2000,22(增刊):25-27
[96] Hurt A C, Barr I G, Komadna N, et al. A novel means of identifying the neuraminidase type of currently circulating human A (H1) influenza viruses [J]. Virus Res, 2004, 103:79-83
[97] Shafer, A. L.,J. B. Katz, and K. A. Eernisse. 1998. Development and validation of a competitive enzymelinked immunosorbent assay for detection of type A influenza antibodies in avian sera. Avian Dis 42:28-34
[98] Zhou E.M., M.Chan, R.A. Heckert, J.Riva, and M. F.Cantin.1998.Evaluation of a competitive ELISA for detection of antibodies against avian nfluenza virus nucleoprotein. Avian Dis 42:142-149
[99]曲站红,邓国华,王桂芹,张立春,田国彬,于康震,陈化兰,. H5亚型高致病性禽流感病毒抗原捕捉ELISA诊断方法的建立[J].中国预防兽医学报,2008,(1):68-71
[100]韩庆功,崔艳红,张智勇. ELISA技术在禽流感诊断研究中的应用[J].生物技术通报,2008,(6):73-77
[101]李海燕,辛晓光,田国斌,等禽流感抗体斑点- ELISA诊断技术的研究.[J].中国预防兽医学报,1999,21(5):321-325
[102]李海燕,于康震,辛晓光,等.禽流感病毒重组核蛋白ELISA诊断技术的研究[J]中国预防兽医学报,2000,22(3):182-185
[103]王传彬,田新生,曹振等. H5亚型禽流感病毒单抗—生物素捕获ELISA的建立. [J].中国实验动物学报,2004,12(4):204-207
[104] Munch M, Nielsen LP, Handberg KJ, et al. Detection and subtyping (H5andH7) of avian type a influenza virus by reverse transcription-PCR and PCR-ELISA. ArchVirol,2001,146:87-97
[105]Dybkaer K, Munch M, Handberg KJ, et al. Application and evaluation of RT-PCR-ELISA for the nucleoprotein and RT-PCR for detection of low pathogenic H5 and H7 subtypes of avian influenza virus. J Vet Diagn Invest, 2004, 16: 51-56.
[106]庞耀珊,谢芝勋,邓显文等.多重反转录聚合酶链反应检测H5亚型禽流感病毒方法的建立.[J].中国人兽共患病杂志, 2005,21(9):762-764
[107]吕长荣,乔海莲,杨增岐,陈杰,顾节清,李晓成.一步法多重RT-PCR禽流感病毒分型诊断法的建立与应用[J].西北农林科技大学学报(自然科学版), 2007, (4):63-67
[108] Collins R A, KoL S, SoK L, et al. Detection of highly pathogenic and low pathogenic avian influenza subtype H5 (Eurasian lineage) using NASBA. J Virol Methods, 2002,103:213-225
[109] Collins R A, KoL S., FungK Y, et al. Rapid and sensitive detection of avian influenza virus subtype H7 using NASBA. [J].Biochem Biophy Res Commun, 2003, 300: 507-515
[110] Shan S, KoL S, Collins R A, et al. Comparison of nucleic acid-based detection of avian influenza H5N1 with virus isolation. [J]. Biochem Biophy Res Commun, 2003, 302: 377-383
[111] Collins R A, KoL S, SoK L, et al. A NASBA method to detect high- and low- pathogenicity H5 avian influenza viruses. [J].Avian Dis,2003,47:1069-1074
[112] Lau L T, Bank J, Aherne R, et al. Nucleic acid sequence-based amplification methods to detect avian influenza virus. [J].Biochem Biophy Res Commun, 2004, 313: 336-342
[113]王楠,邹明强,汪明,李锦丰,薛强,张帆,.禽流感病毒流式微球量子点探针免疫诊断新方法[J].分析测试学报,2009,(7):764-768
[114]石霖,王秀荣,杨忠苹,陶启蒙,王煜,武嘉男,蔡冬冬,包红梅,陈化兰,.禽流感、新城疫可目视化诊断蛋白芯片的制备及初步应用[J].中国预防兽医学报,2009,(1):60-64
[115]崔仑标,唐震,曾晓燕,史智扬,焦永军,葛以跃,马明,单云峰,吴涛,陈银,李显,汪华,.液相芯片技术在诊断江苏省首例人禽流感病例中的应用[J].现代预防医学,2008,(23):4666-4667
[116]张鹤晓,赖平安,刘环,等.荧光RT-PCR检测活禽和禽组织中禽流感病毒的研究.[J].检验检疫科学,2004,14(1):1-5
[117] World Health Organization. Anti-tuberculosis drug resistance in the world. Report No.2. Prevalence and trends. Geneva:WHO,2000
[118]肖东楼.中国结核病防治工作进展与成就.[J].中国防痨杂志.2008,30(6):491-493
[119]全国结核病流行病学抽样调查技术指导组.第四次全国结核病流行病学抽样调查报告.[J].中华结核和呼吸杂志.2002,25(1):3-7
[120]程渝,李茂全.结核分枝杆菌的实验室诊断进展.[J].国外医学临床生物化学与检验学分册.2002,23(6):342-343
[121]周丽荣,罗永爱.结核病血清学诊断的新进展.[J].中国实用内科杂志.2005,3(25):296-271
[122]姜永忠.结核病的特异性免疫诊断.[J].国外医学预防、诊断、治疗用生物品分册.2001, 24 (3): 116-120.
[123] Cole ST, Brosch R, Parkhill J, et al. Deciphering the biology of mycobacterium tuberculosis from the complete genome sequence .Nature.1998, 393(6685):537-544
[124] Alito A, Me Nair J, Girvin RM. Identification of Mycobacterium bovis antigens by analysis of bovin T-cell responses after infection with a virulent strain, Braz.[J].Med Bio Res. 2003, 36: 1523-1531.
[125] Laurens A. H.van Pinxteren, Pernille Ravn, Elea Marie Agger, et al. Diagnosis of Tuberculosis based on the Two Specific Antigens ESAT-6 and CFP10 [J].Clin Diagn Lab Immunol, 2000, 7: 155-160
[126] HarboeM, OettingerT, WikerHG, et al. Evidence for occurence of the ESAT-6 protein in mycobacterium tuberculosis and virulent Mycobacterium bovis and for its absence in Mycobacterium bovis BCG [J]. Infect Immun. 1996, 64 (1):16-22
[127] Mahairas G G, Sabo P J, hickey M J, et al. Molecular analysis of genetic differences between Mycobacterium bovis BCG and virulent M. bovis [J]. Bacteriol ,1996 ,178 :1274～1282
[128] Sandra M Arend , Peter Andersen , Krista E. van Meijgaarden , et al. Detection of active tuberculosis infection by T-cell response to early-secreted antigenic target 62kDa protein and culture filtrate protein 10 [J] . The Journal of Infectious Diseases , 2000 , 181 :1850～1854
[129]李娟,吴雪琼,张俊仙等.结核分枝杆菌CFP10-ESAT6融合蛋白在大肠杆菌中的高效表达.[J].中国防痨杂志.2004,26(4):204-208
[130] Dillon DC, Alderson MR , Day CH , et al . Molecular and immunological characterization of Mycobacterium tuberculosis CFP– 10, An Immunodiagnostic Antigen Missing in Mycobacterium bovis BCG .[J].Clin Micro biol , 2000 , 38 (9) :3285
[131] Wiliins E G, Ivanyi J. Potential value of serology for diagnosis of extrapulmonary tuberculosis [J ].Medicine Science ,1990 ,336 :641～644
[132] Zhou A T, Ma WL ,Zhang P Y, et al . Detection of pulmonary and extrapulmonary tuberculosis patients with the 38 - kilodalton antigen from Mycobacterium tuberculosis in a rapid membrane based assay[J].Clinic Diagnostic Laboratory Immunology ,1996 ,3 :337～341
[133] Nakamura RM, Velmonte MA, Kawajiri K, et al.MPB64 mycobacterial antigen: a new skin-test reagent through patch method for rapid diagnosis of active tuberculosis. Int [J]. Tuberc. Lung Dis, 1998, 2(7):541
[134]李香兰,吴雪琼,张广宇等.结核分支杆菌分泌蛋白Mtb81在大肠杆菌中的高效表达. [J].河北医科大学学报.2005,26(1):25-28
[135] Raymond L , Houghton M, Davin C , et al . Use of multiepitope polyproteins in serodignosis of active tuberculosis[J ] . Clin Dinga Lab Immunol , 2002 , 9 (4) : 883-891.
[136] Lim JH, Park JK, et al. Purification and immunoreactivity of three components from the 30/32-kilodalton antigen 85 complex in Mycobacterium tuberculosis.[J]. Infect Immum, 1999, 67 (11): 6187
[137] Wiker H G, Harboe M. The antigen 85 complex: A major secretion product of Mycobacterium tuberculosis. [J]. Microbiol Rev, 1992, 56:648-661
[138]范雄林,徐志凯,李元等.结核分枝杆菌Ag85B分泌蛋白基因疫苗的构建和免疫原性的研究. [J].中华结核和呼吸杂志,2001,24:548-550
[139] Xinmiao Fu, Zengyi Chang. Temperature-dependent subunit exchange and chaperone -like activities of Hsp16. 3, a small heatshock protein from Mycobacterium tuberculosis [J]. Biochemical and Biophysical Res Commun, 2004, 316 (4) : 291.
[140] Agrewala JN, Wilkinson RJ. Differential regulation of Th1 and Th2 cell by p91-110 and p21-40 peptides of the 16-kD alpha-crystallin antigen of Mycobacterium tuberculosis. [J]. Clin Exp Immunol.1998,114 (3) : 392-397.
[141]徐磊,李慧云,马立恒.四种结核分枝杆菌特异性抗原的克隆表达与血清鉴定.[J].中华结核和呼吸杂志. 2004,27(3):188-190
[142] Lyashchenko K,Colangeli R , Houde M , et al. Heterogeneous antibody responses in tuberculosis. Infect Immun ,1998 ,66 (8) :3936-3940
[143]吴雪琼,张俊仙,夏湘萱等.结核分支杆菌MPT63抗原的表达及其血清学诊断价值的研究.[J].中国现代医学杂志.2001,11(4):8-11
[144] Li H, U lstrup JC, Tonassen TO , et al. Evidence for the absence of the M PB64 gene in some substrains of M ycobacterium bovis BCG . Infect Immun, 1993, 61: 1730-1734.
[145]吴雪琼,张俊仙,李洪敏等.结核分枝杆菌MPT64蛋白的表达,纯化及初步应用[J].中国防痨杂志,2001,23(2):85-88
[146]熊志红,庄玉辉,张晓刚等.结核分支杆菌MPT64基因的克隆及其在大肠肝菌中的表达、纯化和诊断运用.[J].中国现代医学杂志.2004,14(21):48-51
[147] Okud Y, Maekura R, Hirotani A, et al. Rapid serodiagnosis of active pulmonary Mycobacterium tuberculosis by analysis of results from multiple antigen-specific tests.[J].Clin Microbiol, 2004,42(3):1136
[148]崔君兆.弓形虫病研究九十年[J].实用寄生虫病杂志,2000,8(2).
[149]于恩庶主编.弓形虫病学(第1版)[M].福州,福建科学技术出版社,1992.
[150] Savva, D., Morris, J. C., Johnson, J. D., and Hillman. Polymerase chain reaction for detection of Toxoplasma gondii. [J]. Med. Microbiol,1990,32:25–31.
[151]程玉芝,韩广东.弓形虫病的临床诊断. [J].中国寄生虫病防治杂志,1990,3(2):157
[152]吕元聪,崔君兆.我国部分地区弓形虫病流行病学监测.[J].实用寄生虫病杂志,1994,2(3):19-22
[153]林敏,吴少庭,高世同,等.深圳人群弓形虫感染的血清流行病学调查研究[J].寄生虫与医学昆虫学报,2000,7(3):135-139
[154]赵玉强,郑雅梅,闫歌,等.山东地区饲养动物与接触动物职业者弓形虫感染调查.[J].中国热带医学.2001,1(1):69
[155]王崇功,周永华.弓形虫感染对优生优育危害性及其控制的研究[J].中国血吸虫病防治杂志,1998,10(6):362-365
[156]刘爱芹,张唯哲,王光岳等.不明原因智力低下学生弓形虫感染的调查.[J].哈尔滨医科大学学报,1998,12(6):409
[157]王海琦,杜青,倪黎等.孕妇感染弓形虫影响胎儿发育跟踪研究[J].中国人兽共患病杂志,1997,13(2):48-50
[158]邹孟红,周雅,徐惠颖等.未婚妊娠妇女的心理行为和社会因素调查[J].中国心理卫生杂志.1997,11(1):15-17
[159]杨迎工,韩胜凯,高虹等.不孕症与弓形虫感染的检测意义[J].实用男科杂志. 1995,1(1):49-50.
[160]马济宏,郭汉琴,蔡云平.不孕症患者弓形虫抗体检测与分析[J].中国人兽共患病杂志.1996,12(5):封3.
[161]周永华,陆永娟,王瑞兵,等.苏州市农村不孕症夫妇弓形虫感染调查[J].中华男科学,2002,8(5):350-352
[162]黄自平,黄征杰,郭淮,等.140例男性不育病人精浆抗弓形虫抗体检测结果分析[J].中国计划生育学杂志,1998,4:160-161
[163] Marrinez AJ,Sell M,Mitrovices T,et al. The neuropathology and epidemiology of AIDS. A review of 200 cases. [J].Pathol Res Pract,1995,191:427-443
[164] Carey RM,Kimball AC,Armstring D,et al.Toxoplasmosis clinical experience in a cancer hospital . [J].Am J Med,1973,54:30-38
[165]彭丽娟,鲍杨漪,许兰芳等.恶性肿瘤患者弓形虫抗体的调查[J].中国寄生虫学与寄生虫病杂志.1996,14:25
[166]王宝林,潘孝彰,尹有宽等.免疫缺陷性疾病患者弓形虫抗体的调查[J].中国寄生虫学与寄生虫病杂志.2000,18(4):224-226
[167]刘爱芹,李雅杰.不同临床类型的恶性肿瘤患者弓形虫感染调查[J].中国人兽共患病杂志.1998,14(6):73-74
[168]卢致民,张振明,牛发良,等.肿瘤患者弓形虫感染调查[J].中国寄生虫病防治杂志,2003,16(4):203,207
[169]卢慎,郭琴荣.弓形虫病的实验室诊断.[J].医师进修杂志,1987,10(7):28
[170]李允鹤主编.寄生虫病免疫学及免疫诊断(第1版)[M].江苏,江苏科学技术出版社.1991.
[171]李观娣,杜重波,陈义民,等.弓形虫在培养细胞中生物学特性的观察.[J].中国兽医科技,1986,4:10
[172]魏梅雄,张述义,郑思民,等.弓形虫病直接凝集试验的研究.[J].中国人兽共患病杂志,1996,12(1):19
[173]杜惠芬等.弓形虫金标检测卡研制,未发表资料.
[174]陈晓光,刘国章,王洵章,等.含弓形虫P30基因插入昆虫杆状病毒的研究.[J].中国寄生虫学与寄生虫病杂志,1996,14(2):270-273
[175]陈晓光,刘国章,唐银明,等.弓形虫巢式PCR体系的建立及其对人、鼠弓形虫病的检测.[J].中国寄生虫病防治杂志,1996,9(1):53-54
[176]孙丽萍,马静.人H5N1病毒感染及其防治现状[J]..中国预防医学杂志,2007, 8(6): 764-769.
[177] Capua I, Alexander DJ. Human health implications of avian influenza viruses and paramyxoviruses [J]. Eur J Clin Microbiol Infect Dis, 2004, 23:1-6.
[178]廖华乐.禽流感病毒与人类健康[J].中国食品卫生杂志,,1998,10(5):
[179]马鸣潇,金宁一,王振国,等.检测禽流感病毒RT-PCR一步法的建立及H5、H9亚型的鉴定[J].中国生物制品学杂志,2005, 18(5): 415-417.
[180]魏泉德,谭爱军.禽流感病毒实验检测研究进展[J].微生物学通报,2007, 34(5): 986-990.
[181]刘维全,刘海鹏,江禹,等.禽流感病毒、新城疫病毒、猪瘟病毒和口蹄疫病毒多联RT-PCR诊断技术的建立[J].农业生物技术学报,2005, 13(1): 92-95.
[182]韩剑锋,宁宜宝,宋立,等.实时荧光PCR技术快速鉴别检测H5、H9、H7亚型禽流感灭活疫苗的研究[J].生物工程学报,2007, 23(5): 953-957.
[183]韩刚毅.胶体金标记抗体技术[J].生物化学与生物物理进展,1989, 16(1): 31-35.
[184] Houiman RE, Raymond R, Renton N, et al. The diagnosis of toxoplasmosis using IgG avidity[J]. Epidemiol Infect, 1994, 112: 399-408.
[185]邵军军.胶体金免疫层析试纸条在病原体检测及医学诊断中的应用[J].实用诊断与治疗杂志,2008, 22(1): 39-40.
[186] Kumar H, Malhotra D, Goswami S, et al. How far have we reached in Tuberculosis vaccine development[J]. Crit rev microbiol, 2003, 29 (4): 297-312.
[187] Anderson AB, Hansen EB. Structure and mapping of antigenic domains of protein antigen b, a 38 kb molecular weight protein of Mycobacterium tuberculosis[J].. Infect Immun, 1989, 57: 2481-2488.
[188] Ivanyi J, BothamLey GH, Jackett PS, et al. Immuno-diagnostic assays for tuberculosis and leprosy[J]. Br Med Bull, 1988, 44: 635-649.
[189] Kingston AE, Salgame PR, Mitchisoa A, et al. Immunological activity of a 14 kilodalton recombinant protein of Mycobacterium tuberculosis H37RV[J]. Infect Immun, 1987, 55: 3149-3154.
[190] Lee BY, Hefta SA, Brennan PJ. Characterization of the major membrane protein of virulent Mycobacterium tuberculosis[J]. Infect Immun, 1992, 60: 2066-2074.
[191] Fu X, Chang Z. Temperature-dependent subunit exchange and chaperone-like activities ofHsp16.3, a small heatshock protein from Mycobacterium tuberculosis[J]. Biochem Biophys Res Commun, 2004, 316 (2): 291-299.
[192] Verstijnen CP, Scokmingh R, Kuijper S, et al. Rapid identification of cultural Mycobacterium tuberculosis with a panel of monoclonal antibodies in Western blot and immunofluorescence [J]. Res Microbiol, 1989, 140: 653-666.
[193] Hu Y, Philip D. Protein synthesis is shutdown in dormat Mycobacterium tuberculosis and is reversed by oxygen or heat shock[J]. FEMS Microbiol Lett, 1998, 158 (1): 139.
[194] BothamLey GH, Rudd R, Festenstein F, et al. Clinical value of the measurement of Mycobacterium tuberculosis specific antibody in pulmonary tuberculosis[J]. Thorax, 1992, 47(4): 270
[195]熊志红,庄玉辉,张晓刚,等.结核分枝杆菌16000抗原基因在大肠杆菌中的高效表达及其产物纯化[J].中华结核和呼吸杂志,1999, 22(11): 645-647.
[196]杨军兰,杨侠.结核分枝杆菌16KD蛋白的基因克隆及表达与纯化[J].实用医技杂志,2008, 15(29): 4001-4003.
[197] Yong D, Kent L. Immunoligic activty of a 38-kilodaton protein purified from Mycobacterium tuberculosis[J]. Infect Immun, 1986, 54(1): 177-183.
[198] Andersen AB, Ljungqvist L, Olsen M, et al. Evidence that protein anigen of mycobacterium tuberculosis is invloved in phosphste metabolism[J]. Gen microbiol, 1990, 136: 477-480.
[199] Chang Z, Abha C, Raju L, et al. The immunodominant 38 kDa liproprotein antigen of Mycobacterium tuberculosis is a phosphate binding protein. J Biol Chem, 1994, 269: 1956-1958.
[200] Kadival GV, Chaparas SD, Hussong D. Characterization of serologic and cell mediated reactivity of a 38 kDa antigen isolated from Mycobacterium tuberculosis[J]. J Immun, 1987, 139: 2447-2451.
[201] Singh M, Andersen AB, Mclarthy JE, et al. The Mycobacterium tuberculosis 38 kDa antigen: overexpression in Escherichia coli, purification and characterization[J]. Gene, 1992, 117: 53-60.
[202] Souza CD, Kadival GV, Samuel AM. Use of the recombinant 38 kDa antigen of Mycobacterium tuberculosis as an immunogen for specific antisera production[J]. Microbiol Immun, 1994, 38: 797-800.
[203] Haslov K, Andersen AB, Ljungqvist L, et al. Comparison of the immunological activity of five defined antigens from Mycobacterium tuberculosis in seven inbred guinea pig strains, the 38 kDa antigen is immunodominant[J]. Scand J Immun, 1990, 31: 503-514.
[204] Wilkinson RJ, Haslov K, Rappuoli R, et al. Evaluation of the recombinant 38 kilodalton antigen of Mycobacterium tuberculosis as a potential immunodiagnostic reagent[J]. J Clin Microbiol, 1997, 35: 553-557.
[205]何秀云,庄玉辉,张晓刚,等.结核分枝杆菌38000蛋白质抗原在大肠杆菌中高效表达[J].中华结核和呼吸杂志,1999, 22 (3): 138-141.
[206]吴燕,朱中元,王海波.结核分枝杆菌38KD蛋白基因克隆及在大肠杆菌中的表达[J].中国热带医学,2006, 5(9):1786-1789.
[207]苏明权,岳乔红,周萍等,结核分枝杆菌重组蛋白的制备及抗原性分析[J].第四军医大学学报,2006,27(11): 987-990.
[208]周丽蓉,徐敬华,罗永爱,等.结核分枝杆菌38 ku蛋白的制备及抗原性研究[J].中国实用内科杂志,2007,27(24): 1955-1957.
[209]刘贤杰,朱中元,陈奎霖.重组结核分支杆菌38KDa蛋白抗原制备及其血清学诊断价值[J].中国热带医学,2008, 8(8): 1275-1277.
[210] He XY, Luo YA, Zhang XG, et al. Clinical evaluation of the recombinant 38 kDa protein of Mycobacterium tuberculosis[J]. Scand J Infect Dis, 2008, 40(2): 121-126.
[211]朱中元,王海波,刘海国,等.抗结核分支杆菌多种抗原的抗体检测蛋白芯片研究[J].中国热带医学,2004, 4(6): 907-910.
[212]张小刚,庄玉辉,何秀云,等.重组结核分支杆菌38kD和16kD蛋白用于结核病血清学诊断的价值[J].中国防痨杂志,2001, 23 (5): 183.
[213]汪明.兽医寄生虫学[M].第三版,北京:中国农业出版社, 2005.
[214]刘德纯,林清森,Seshan V.获得性免疫缺陷综合症(AIDS)合并播散性弓形虫病尸检的病理学研究[J].中华病理学杂志, 1994, 23(3): 166-169.
[215] Tsuda Y, Sakoda Y, Sakabe S, et al. Development of an immunochromatographic kit for rapid diagnosis of h5 avian influenza virus infection[J]. Micrology and immunology, 2007, 51: 903-907
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |