Effect of low temperatures on BAX and BCL2 proteins in rats with spinal cord ischemia reperfusion injury

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• 英文论文题名：Effect of low temperatures on BAX and BCL2 proteins in rats with spinal cord ischemia reperfusion injury
• 论文作者：Zhu Ping ; Zhao M.y. ; Li X.h. ; Fu Q. ; Zhou Z.f. ; Huang C.f. ; Zhang X.s. ; Huang H.l. ; Tan Y. ; Li J.x. ; Li J.n. ; Huang S. ; Ashraf M. ; Lu C. ; Chen J.m. ; Zhuang J. ; Guo H.m.
• 英文论文作者：Zhu Ping ; Zhao M.y. ; Li X.h. ; Fu Q. ; Zhou Z.f. ; Huang C.f. ; Zhang X.s. ; Huang H.l. ; Tan Y. ; Li J.x. ; Li J.n. ; Huang S. ; Ashraf M. ; Lu C. ; Chen J.m. ; Zhuang J. ; Guo H.m. ; Department of Cardiovascular Surgery ; Guangdong General Hospital ; Guangdong Cardiovascular Institute ; Guangdong Academy of Medical Sciences ; Department of Pediatrics ; The Third Xiangya Hospital ; Central South University ; Department of Pharmacology ; UIC College of Medicine
• 年：2016
• 作者机构：Department of Cardiovascular Surgery,Guangdong General Hospital,Guangdong Cardiovascular Institute,Guangdong Academy of Medical Sciences;Department of Pediatrics,The Third Xiangya Hospital,Central South University;Department of Pharmacology,UIC College of Medicine;
• 英文论文关键词：Hypothermia ; Spinal cord ischemia/reperfusion injury ; BAX ; BCL2
• 会议召开时间：2016-11-04
• 会议录名称：第十一届全国免疫学学术大会摘要汇编
• 英文会议录名称：Abstracts of 2016 CSI Congress on Immunology
• 语种：英文
• 分类号：R651.2
• 学会代码：IGMD
• 会议名称：第十一届全国免疫学学术大会
• 会议地点：中国安徽合肥
• 主办单位：中国免疫学会
• 学会名称：中国免疫学会
• 页数：1
• 文件大小：314k
• 原文格式：D
• 会议级别：全国

摘要

Objectives: Surgery on the chest often requires blocking of the abdominal aorta. However, the associated spinal cord ischemia/reperfusion injury(SCII) can cause paraplegia in severe cases. Conducting heart operations at low temperatures as a means of protecting the central nervous system. We report our preliminary investigation of the neuroprotective effect of different temperatures on the rat spinal SCII model. Our Objective was to determine whether the protective effect is closely related to the inhibition of apoptosis in nerve cells to improve the SCII protection method. Methods: We evaluated changes in BAX and BCL2 expression levels after spinal cord ischemia/reperfusion injury(SCII) and hypothermia during operations in rats. Eighty rats were divided into four groups: Group A(N = 20, 18°C); GroupB(N = 20, 28°C); Group C(N = 20, room temperature); and Group D(N = 20, sham operation control). Spinal cord ischemia was induced for 90 min. Hypothermia was induced 15 min before, and maintained during ischemia, followed by heating to normothermia for 30 min after reperfusion. Motor function of the lower limbs was evaluated according to the Tarlov score at 72 and 168 h. For each rat, spinal cord samples were taken at 6, 24, 72 h, and 1 week to evaluate the histopathological changes, neuronal apoptosis, and BAX and BCL2 expression levels. Reaults: Compared with normothermia, hypothermia significantly improved hind limb function; GroupB achieved a higher score than Group A. Group D showed no neurologic deficiency, while the other groups showed various degrees. Group C exhibited greater neuronal apoptosis, higher BAX expression, but lower BCL2 expression than the other groups. Compared with Group A, BAX was expressed less and BCL2 more in GroupB, and there was less apoptosis in GroupB. Conclusion: Hypothermia preserves hind limb motor function and reduces neuronal death, thereby protecting rats from SCII. The spinal cord may be protected from SCII by inhibition of BAX and activation of BCL2.
Objectives: Surgery on the chest often requires blocking of the abdominal aorta. However, the associated spinal cord ischemia/reperfusion injury(SCII) can cause paraplegia in severe cases. Conducting heart operations at low temperatures as a means of protecting the central nervous system. We report our preliminary investigation of the neuroprotective effect of different temperatures on the rat spinal SCII model. Our Objective was to determine whether the protective effect is closely related to the inhibition of apoptosis in nerve cells to improve the SCII protection method. Methods: We evaluated changes in BAX and BCL2 expression levels after spinal cord ischemia/reperfusion injury(SCII) and hypothermia during operations in rats. Eighty rats were divided into four groups: Group A(N = 20, 18°C); GroupB(N = 20, 28°C); Group C(N = 20, room temperature); and Group D(N = 20, sham operation control). Spinal cord ischemia was induced for 90 min. Hypothermia was induced 15 min before, and maintained during ischemia, followed by heating to normothermia for 30 min after reperfusion. Motor function of the lower limbs was evaluated according to the Tarlov score at 72 and 168 h. For each rat, spinal cord samples were taken at 6, 24, 72 h, and 1 week to evaluate the histopathological changes, neuronal apoptosis, and BAX and BCL2 expression levels. Reaults: Compared with normothermia, hypothermia significantly improved hind limb function; GroupB achieved a higher score than Group A. Group D showed no neurologic deficiency, while the other groups showed various degrees. Group C exhibited greater neuronal apoptosis, higher BAX expression, but lower BCL2 expression than the other groups. Compared with Group A, BAX was expressed less and BCL2 more in GroupB, and there was less apoptosis in GroupB. Conclusion: Hypothermia preserves hind limb motor function and reduces neuronal death, thereby protecting rats from SCII. The spinal cord may be protected from SCII by inhibition of BAX and activation of BCL2.

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