糖尿病大鼠胃平滑肌中PDE3的表达及活性的改变

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• 英文论文题名：Expression and activity of PDE3 in the diabetic rats gastric smooth muscle
• 论文作者：赵洪伟 ; 郑嫦 ; 李成浩
• 英文论文作者：ZHAO Hong-wei ; ZHENG Chang ; LI Cheng-hao ; Department of Anatomy ; Yanbian University Medical College ; Yanbian University Hospital
• 年：2017
• 作者机构：延边大学医学院解剖学教研室;延边大学附属医院;
• 论文关键词：糖尿病 ; 胃动力障碍 ; PDE3
• 英文论文关键词：diabetic ; gastric motility disorder ; PDE3
• 会议召开时间：2017-08-07
• 会议录名称：中国解剖学会第六届全国解剖学技术学术会议论文汇编
• 语种：中文
• 分类号：R587.2
• 学会代码：ZGJP
• 会议名称：中国解剖学会第六届全国解剖学技术学术会议
• 会议地点：中国江西九江
• 主办单位：中国解剖学会科技开发和咨询工作委员会
• 学会名称：中国解剖学会
• 页数：3
• 文件大小：1873k
• 原文格式：D
• 会议级别：全国

摘要

目的观察糖尿病胃平滑肌组织中磷酸二酯酶3(PDE3)的表达及活性变化。方法腹腔注射链脲佐菌素制备糖尿病模型,造模成功4周后利用免疫组化、western blot和高效液相色谱(HPLC)检测胃平滑肌组织中PDE3表达和活性变化。结果免疫组化和western blot结果PDE3在胃平滑肌中均表达,且正常组与糖尿病组之间无明显的改变;且其活性显著降低。结论胃平滑肌组织中PDE3活性降低是糖尿病胃动力障碍机制之一。
Objective In order to explore the expression and activity of phosphodiesterase-3(PDE3)in the diabetic rats gastric smooth muscle.Method The diabetic model by intraperitoneal injection of STZ,after 4 weeks PDE3 expression was detected by immunohistochemistry and Western blot,PDE3 activity of gastric smooth muscle tissue was detected by HPLC.Results Immunohistochemistry and Western blot results showed that the expression of PDE3 in gastric smooth muscle was normal,but there was no significant difference between normal group and diabetic group.Conclusion The decrease of PDE3 activity in gastric smooth muscle is one of the mechanisms of diabetic gastric motility disorder

引文

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