基于形态鉴定及叶片解剖结构对云南白莺山古茶树资源的聚类分析
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- 英文论文题名：Clustering analysis of ancient tea plants in Yunnan Baiying mountain based on morphological identification and leaf anatomical structure
- 论文作者：李崇兴
- 英文论文作者：LI Chong-xing ; Tea Research Institute of Lincang
- 年：2017
- 作者机构：临沧市茶叶科学研究所;
- 论文关键词：古茶树 ; 种质资源 ; 形态 ; 解剖结构 ; 聚类分析
- 英文论文关键词：Ancient tea plant ; Germplasm resource ; Morphology ; Anatomical structure ; Clustering analysis
- 会议召开时间：2017-09-25
- 会议录名称：第七届云南省科协学术年会论文集——专题一：科普助力精准扶贫
- 语种：中文
- 分类号：S571.1
- 学会代码：SSAD
- 会议名称：第七届云南省科协学术年会
- 会议地点：中国云南普洱
- 主办单位：云南省科学技术协会、中共普洱市委、普洱市人民政府
- 学会名称：云南省机械工程学会
- 页数：13
- 文件大小：683k
- 原文格式：D
- 会议级别：地方
摘要
对云南白莺山地区12种类型古茶树资源共60份材料进行形态及叶片解剖结构研究,在此基础上,构建系统聚类图分析这12类茶树的亲缘关系,旨在为今后深入研究白莺山古茶树资源的起源与演化、加快珍稀古茶树资源的开发利用提供形态学和细胞学的参考依据。研究结果表明,12类古茶树可分为两大类和四小类,野生型的本山茶与3个半野生型的黑条子茶、二嘎子茶和大叶本山白芽口茶归为第Ⅰ类,为乔木或小乔木,树型高大,芽叶茸毛无或稀少,叶片大,角质层较厚,栅栏细胞1层,石细胞多且粗大;其他8个类型归为第Ⅱ类,其中4个栽培类型包括勐库大叶茶、勐库小叶茶、白芽子茶和豆蔑茶归为第Ⅱ-a类,为小乔木或灌木,芽叶茸毛较多,叶片栅栏细胞1-2层,石细胞少且小;柳叶茶、贺庆茶、藤子茶3个半野生类型资源归为第Ⅱ-b类,为灌木,树型开张,芽叶茸毛中等,中叶,具有1-2层栅栏细胞,石细胞少且小;红芽茶单独成第Ⅱ-c类,为半野生型灌木,芽叶呈鲜艳紫红色,大叶,石细胞形态和大小多样。
In this study,the morphology and leaf anatomical structure of 12 kinds of ancient tea plants including 60 germplasms were identified and analyzed.On this base,a dendrogram of these ancient tea plants was established and genetic relationship among them was analyzed,which would provide morphological and cytological evidences for lucubrating their origination and evolution,as well as accelerating their development and utilization.The results indicate that,12 kinds of gemplasms are clustered into two main groups and four subclasses.Group Ⅰ consists of one wild type( Benshan) and three semi-wild types( Heitiaozi,Ergazi and Dayebenshanbaiyakou),which are tall arbors or semi-arbors with absent or sparse young shoot pubescences,large leaves,thicker cuticles,one lay of palisade cells,and many bulky sclereids.Four cultivated types( Menkudaye,Menkuxiaoye,Baiyazi and Doumie) belong to group Ⅱ-a,which are semi-arbors or shrubs with denser young shoot pubescences,one to two layers of palisade cells,as well as few small sclereids.Three semi-wild types( Liuye,Heqing and Tengzi) are classed into group Ⅱ-b,which are horizontal spreading shrubs with medium young shoot pubescences,medium leaves,one to two layers of palisade cells,and few small sclereids.Group Ⅱ-c has only one member,Hongya,which is semi-wild shrub with bright reddish violet young shoots,large leaves,and diversifying sclereids in morphology and size.
In this study,the morphology and leaf anatomical structure of 12 kinds of ancient tea plants including 60 germplasms were identified and analyzed.On this base,a dendrogram of these ancient tea plants was established and genetic relationship among them was analyzed,which would provide morphological and cytological evidences for lucubrating their origination and evolution,as well as accelerating their development and utilization.The results indicate that,12 kinds of gemplasms are clustered into two main groups and four subclasses.Group Ⅰ consists of one wild type( Benshan) and three semi-wild types( Heitiaozi,Ergazi and Dayebenshanbaiyakou),which are tall arbors or semi-arbors with absent or sparse young shoot pubescences,large leaves,thicker cuticles,one lay of palisade cells,and many bulky sclereids.Four cultivated types( Menkudaye,Menkuxiaoye,Baiyazi and Doumie) belong to group Ⅱ-a,which are semi-arbors or shrubs with denser young shoot pubescences,one to two layers of palisade cells,as well as few small sclereids.Three semi-wild types( Liuye,Heqing and Tengzi) are classed into group Ⅱ-b,which are horizontal spreading shrubs with medium young shoot pubescences,medium leaves,one to two layers of palisade cells,and few small sclereids.Group Ⅱ-c has only one member,Hongya,which is semi-wild shrub with bright reddish violet young shoots,large leaves,and diversifying sclereids in morphology and size.
引文
[1]唐一春,王平盛,许玫,等.云南云县白莺山古茶资源考察[J].中国茶业,2007,5:24-25
[2]张颖君,杨崇仁,曾恕芬,等.白莺山古茶的化学成分分析与栽培茶树的起源[J].云南植物研究,2010,32(1):77-82
[3]陈亮,杨亚军,虞富莲.中国茶树种质资源研究的主要进展和展望[J].植物遗传资源学报,2004,5(4):389-392
[4]Liu B Y,Song W X,Sun X M.Documentation concordance,sharing and utilization of tea germplasm resources in Yunnan[J].Agricultural Science&Technology,2011,12(12):1842-1848
[5]束际林.茶树叶片解剖结构鉴定的原理与技术[J].中国茶业,1995,1:2-4
[6]束际林.云南省三大古茶树叶片解剖结构及花粉形态的研究[J].中国茶业,1994,3:15-17
[7]房用,孟振农,李秀芬,等.山东茶树叶片解剖结构分析[J].茶叶科学,2004,24(3):190-196
[8]李瑞,肖斌,宋红霞,等.50份茶树种质资源农艺性状及其聚类分析[J].西北农业学报,2011,20(10):107-111
[9]王小萍,唐晓波,王迎春,等.52份茶树资源生化组分的表型多样性分析[J].茶叶科学,2012,32(2):129-134
[10]蒋会兵,矣兵,梁名志,等.云南茶树种质资源形态性状多样性分析[J].云南农业大学学报,2011,26(6):833-840
[11]刘宝祥,刘湘呜.云南茶树原产地考察[J].茶业通报,1981,1:11-15
[12]严学成.茶树形态结构与品质鉴定[M].北京:农业出版社,1989,106-122
[13]张宏达.山茶属植物的系统研究[J].中山大学学报(自然科学版)论丛,1981(1):1-124
[14]闵天禄.山茶属茶组植物的订正[J].云南植物研究,1992,14(2):115-132
[15]陈亮,虞富莲,童启庆.关于茶组植物分类与演化的讨论[J].茶叶科学,2000,20(2):89-94
[16]束际林,陈亮,虞富莲,等.茶树优质资源花粉的遗传稳定性[J].茶叶科学.2000,20(1):12-18
[17]Ma JQ,Zhou YH,Ma CL et al.Identification and characterization of 74 novel polymorphic EST-SSR markers in the tea plant,Camellia sinensis(Theaceae)[J].American journal of botany,2010,97(12):e153-e156
[18]Ma JQ,Ma CL,Yao MZ et al.Microsatellite markers from tea plant expressed sequence tags(ESTs)and their applicability for cross-species/genera amplification and genetic mapping[J].Scientia horticulturae,2012,134:167–175
[19]Fang W,Cheng H,Duan Y,et al.Genetic diversity and relationship of clonal tea(Camellia sinensis)cultivars in China as revealed by SSR markers[J].Plant Systematics and Evolution,2012,298:469-483
[20]Yao MZ,Ma CL,Qiao TT,et al.Diversity distribution and population structure of tea germplasms in China revealed by EST-SSR markers[J].Tree Genetics&Genomes,2012,8:205-220
[2]张颖君,杨崇仁,曾恕芬,等.白莺山古茶的化学成分分析与栽培茶树的起源[J].云南植物研究,2010,32(1):77-82
[3]陈亮,杨亚军,虞富莲.中国茶树种质资源研究的主要进展和展望[J].植物遗传资源学报,2004,5(4):389-392
[4]Liu B Y,Song W X,Sun X M.Documentation concordance,sharing and utilization of tea germplasm resources in Yunnan[J].Agricultural Science&Technology,2011,12(12):1842-1848
[5]束际林.茶树叶片解剖结构鉴定的原理与技术[J].中国茶业,1995,1:2-4
[6]束际林.云南省三大古茶树叶片解剖结构及花粉形态的研究[J].中国茶业,1994,3:15-17
[7]房用,孟振农,李秀芬,等.山东茶树叶片解剖结构分析[J].茶叶科学,2004,24(3):190-196
[8]李瑞,肖斌,宋红霞,等.50份茶树种质资源农艺性状及其聚类分析[J].西北农业学报,2011,20(10):107-111
[9]王小萍,唐晓波,王迎春,等.52份茶树资源生化组分的表型多样性分析[J].茶叶科学,2012,32(2):129-134
[10]蒋会兵,矣兵,梁名志,等.云南茶树种质资源形态性状多样性分析[J].云南农业大学学报,2011,26(6):833-840
[11]刘宝祥,刘湘呜.云南茶树原产地考察[J].茶业通报,1981,1:11-15
[12]严学成.茶树形态结构与品质鉴定[M].北京:农业出版社,1989,106-122
[13]张宏达.山茶属植物的系统研究[J].中山大学学报(自然科学版)论丛,1981(1):1-124
[14]闵天禄.山茶属茶组植物的订正[J].云南植物研究,1992,14(2):115-132
[15]陈亮,虞富莲,童启庆.关于茶组植物分类与演化的讨论[J].茶叶科学,2000,20(2):89-94
[16]束际林,陈亮,虞富莲,等.茶树优质资源花粉的遗传稳定性[J].茶叶科学.2000,20(1):12-18
[17]Ma JQ,Zhou YH,Ma CL et al.Identification and characterization of 74 novel polymorphic EST-SSR markers in the tea plant,Camellia sinensis(Theaceae)[J].American journal of botany,2010,97(12):e153-e156
[18]Ma JQ,Ma CL,Yao MZ et al.Microsatellite markers from tea plant expressed sequence tags(ESTs)and their applicability for cross-species/genera amplification and genetic mapping[J].Scientia horticulturae,2012,134:167–175
[19]Fang W,Cheng H,Duan Y,et al.Genetic diversity and relationship of clonal tea(Camellia sinensis)cultivars in China as revealed by SSR markers[J].Plant Systematics and Evolution,2012,298:469-483
[20]Yao MZ,Ma CL,Qiao TT,et al.Diversity distribution and population structure of tea germplasms in China revealed by EST-SSR markers[J].Tree Genetics&Genomes,2012,8:205-220
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