Imbalanced expression of Bcl-xL and Bax in platelets treated with plasma from immune thrombocytopenia

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• 英文论文题名：Imbalanced expression of Bcl-xL and Bax in platelets treated with plasma from immune thrombocytopenia
• 论文作者：Qiao Jianlin ; Wu Yulu ; Li Xiaoqian ; Li Mingfeng ; Li Wen ; Ding Lan ; Zeng Lingyu ; Xu Kailin
• 英文论文作者：Qiao Jianlin ; Wu Yulu ; Li Xiaoqian ; Li Mingfeng ; Li Wen ; Ding Lan ; Zeng Lingyu ; Xu Kailin ; Department of Hematology ; the Affiliated Hospital of Xuzhou Medical University
• 年：2016
• 作者机构：Department of Hematology,the Affiliated Hospital of Xuzhou Medical University;
• 英文论文关键词：Apoptosis ; Bax ; Bcl-xL ; Caspase-3 ; Immune thrombocytopenia
• 会议召开时间：2016-11-04
• 会议录名称：第十一届全国免疫学学术大会摘要汇编
• 英文会议录名称：Abstracts of 2016 CSI Congress on Immunology
• 语种：英文
• 分类号：R558.2
• 学会代码：IGMD
• 会议名称：第十一届全国免疫学学术大会
• 会议地点：中国安徽合肥
• 主办单位：中国免疫学会
• 学会名称：中国免疫学会
• 页数：2
• 文件大小：1333k
• 原文格式：D
• 会议级别：全国

摘要

Aims: To evaluate the expression profile of Bcl-x L and Bax in platelets treated with ITP plasma. Methods:Normal washed platelets were treated with plasma from 20 active ITP patients or 10 age and gender-matched control to mimic the ITP in vivo environment. Mitochondrial depolarization, platelet apoptosis and activation were measured by flow cytometry. Expression of Bcl-x L, Bax and caspase-3 were also measured by quantitative real-time PCR and western blot. Results:Our study demonstrated increased mitochondrial depolarization, platelet apoptosis and activation in platelets after treated with ITP plasma in comparison to control. In addition, decreased expression of Bcl-x L, increased expression of Bax and activity of caspase-3 were also observed. Furthermore, a negative correlation of Bcl-x L with Bax was found in platelets treated with ITP plasma. Conclusions: Imbalanced expression of Bcl-x L and Bax might be associated with platelet apoptosis in ITP and therapeu-tically targeting them might be a novel approach in the treatment of ITP.
Aims: To evaluate the expression profile of Bcl-x L and Bax in platelets treated with ITP plasma. Methods:Normal washed platelets were treated with plasma from 20 active ITP patients or 10 age and gender-matched control to mimic the ITP in vivo environment. Mitochondrial depolarization, platelet apoptosis and activation were measured by flow cytometry. Expression of Bcl-x L, Bax and caspase-3 were also measured by quantitative real-time PCR and western blot. Results:Our study demonstrated increased mitochondrial depolarization, platelet apoptosis and activation in platelets after treated with ITP plasma in comparison to control. In addition, decreased expression of Bcl-x L, increased expression of Bax and activity of caspase-3 were also observed. Furthermore, a negative correlation of Bcl-x L with Bax was found in platelets treated with ITP plasma. Conclusions: Imbalanced expression of Bcl-x L and Bax might be associated with platelet apoptosis in ITP and therapeu-tically targeting them might be a novel approach in the treatment of ITP.

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