棉花中水溶性总糖含量的提取及测定方法研究
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- 英文论文题名：Study on Extraction and Determination Method of Total Water-Soluble Sugars in Cotton
- 论文作者：王延琴 ; 周大云 ; 匡猛 ; 马磊 ; 徐双娇 ; 方丹 ; 王爽
- 英文论文作者：Wang Yanqin ; Zhou Dayun ; Kuang Meng ; Ma Lei ; Xu ShuangJiao ; Fang Dan ; Wang Shuang ; Institute of Cotton Research of Chinese Academy of Agricultural Sciences
- 年：2017
- 作者机构：中国农业科学院棉花研究所/农业部棉花遗传改良重点开放实验室;
- 论文关键词：棉花 ; 水溶性总糖 ; 提取 ; 测定
- 英文论文关键词：cotton ; water-soluble sugar content ; extraction ; determination
- 会议召开时间：2017-08-07
- 会议录名称：中国农学会棉花分会2017年年会暨第九次会员代表大会论文汇编
- 语种：中文
- 分类号：S562
- 学会代码：MHXB
- 会议名称：中国农学会棉花分会2017年年会暨第九次会员代表大会
- 会议地点：中国河南郑州
- 主办单位：中国农学会棉花分会
- 学会名称：《棉花学报》编辑部
- 页数：9
- 文件大小：896k
- 原文格式：O
- 会议级别：全国
摘要
通过对蒽酮比色法测定棉花中水溶性总糖含量的几个问题进行了探讨,优化了蒽酮比色法测定棉花中水溶性总糖含量的反应条件及棉花中水溶性总糖的提取条件。结果表明,蒽酮比色法测定棉花水溶性总糖的适宜测定条件为:波长575 nm,显色剂添加量5 mL,显色时间10 min,静置时间10min~1 h均可,在此条件下葡萄糖标准品浓度与吸光度之间具有良好的线性关系(C=0.131 8A+0.000 4,R~2=0.999 2)。采用此优化条件测定棉花中水溶性总糖含量,灵敏度高(可达到0.003 g·L~(-1)的检出限),具有良好的精度(重复性标准偏差为0.04)和准确性(平均回收率为100.3%)。该测定方法简单、经济、快捷、准确,特别适合大批量样品,尤其是资金有限的实验室。
The reaction conditions and extracting conditions of the anthrone colorimetric method to determine the soluble sugar content of cotton was investigated in this paper.The results showed that the optimum determination conditions were as follows:detector wavelength of 575 nm,reaction time for 10 min,color-display reagent consumption for 5 mL,reaction stalling time for 20-60 min.Under these conditions,there was a good linear relationship between the concentration of glucose and absorbance(C= 0.131 8A+0.000 4,R~2=0.999 2).The limit of detection(LOD) for glucose of the proposed method is 0.003 g·L~(-1).The relative standard deviation(RSD) and the average recovery rate of the method was 0.04 and 100.3%,respectively,which denoted high repeatability and precision.Hence the established method is simple,economical,rapid and accurate,and can be suitable to routine analysis of bulk samples.
The reaction conditions and extracting conditions of the anthrone colorimetric method to determine the soluble sugar content of cotton was investigated in this paper.The results showed that the optimum determination conditions were as follows:detector wavelength of 575 nm,reaction time for 10 min,color-display reagent consumption for 5 mL,reaction stalling time for 20-60 min.Under these conditions,there was a good linear relationship between the concentration of glucose and absorbance(C= 0.131 8A+0.000 4,R~2=0.999 2).The limit of detection(LOD) for glucose of the proposed method is 0.003 g·L~(-1).The relative standard deviation(RSD) and the average recovery rate of the method was 0.04 and 100.3%,respectively,which denoted high repeatability and precision.Hence the established method is simple,economical,rapid and accurate,and can be suitable to routine analysis of bulk samples.
引文
[1]连素梅,袁志清.国内外棉花糖分与粘性检测方法分析[J].中国纤检,2005(4):30-32.
[2]杨长琴,刘瑞显,张国伟,等.花铃期渍水对棉纤维糖代谢的影响及其与纤维比强度的关系[J].作物学报,2014,40(9):1612-1618.
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[8]郭玉华,杨伟华,郁有祝,等.蒽酮比色法测定棉花成熟纤维中水溶性总糖含量[J].2011,48(12):23-26.
[9]周炜,李卫东,周兆懿,等.铁氰化钾法测棉花含糖量的影响因素研究[J].上海毛麻科技,2014(2):29-31.
[10]中华人民共和国国家质量监督检验检疫总局.进出口棉纤维含糖量检验方法第2部分色卡比色法:SN/T 0311.2-2010[S].北京:中国标准出版社,2010.
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[12]刘萍,李明军.植物生理学实验技术[M].北京:科技出版社,2007:86-88.
[13]文赤夫,董爱文,李国章,等.蒽酮比色法测定紫花地丁中总糖及还原糖含量[J].现代食品科技,2005,21(3):122-123.
[14]刘海英,王华华,崔长海,等.可溶性糖含量测定(蒽酮法)实验的改进[J].实验室科学,2013,16(2):19-20.
[15]李晓旭,李家政.优化蒽酮比色法测定甜玉米中可溶性糖的含量[J].保鲜与加工,2013,13(4):24-27.
[16]丁雪梅,张晓君,赵云,等.蒽酮比色法测定可溶性糖含量的试验方法改进[J].实验室检测,2014(12):230-233.
[17]Ah Moradi,Omid Younesi.Influence of arbuscular mycorrhiza on membrane hpid peroxidation and soluble sugar content of soybean under salt stress[J].Agriculturae Conspectus Scientificus,2015,79(4):227-232.
[18]Nayer Mohammadkhani,Reza Heidari.Drought-induced accumulation of soluble sugars and proline in two maize varieties[J].World Apphed Sciences Journal,2008,3(3):448-453.
[19]郭晓蕾,朱思潮,翟旭峰,等.硫酸蒽酮法与硫酸苯酚法测定灵芝多糖含量比较[J].中华中医药学刊,2010,28(9):22-24.
[20]Marjorie R R,Patrick A J,Buddhi P L,et al.Total water soluble sugars quantification in bio-oil using the phenol sulfuric acid assay[J].Journal of Analytical and Apphed Pyrolysis,2013,104:194-201.
[21]祝义伟,周令国,叶宸志,等.香菇柄中可溶性糖的测定[J].农产品加工,2015(3):43-45.
[22]罗志刚,曾满枝,凌晨,等.3,5-二硝基水杨酸比色法测定烟草中水溶性总糖[J].中国烟草科学,2000(2):34-36.
[23]尹建雄,卢红,谢强,等.3,5-二硝基水杨酸比色法快速测定烟草水溶性总糖、还原糖及淀粉的探讨[J].云南农业大学学报,2007,22(6):829-833.
[24]郑荣庆.烟叶中还原糖的斐林试液比色测定法[J].烟草科技,1985(2):26-31.
[25]Liu H R Song H X,Liu D P,et al.Dynamics changes of soluble sugar and free amino acid contents in stem and leaf of different oilseed rape varieties[J].Acta Agriculturae Boreali-Occidentalis Sinica,2007,16(1):123-126.
[26]Kato-Noguchi Hisashi,Yasuda Yukihiro,Sasaki Ryosuke.Soluble sugar availability of aerobically germinated barley,oat and rice coleoptiles in anoxia[J].Journal of Plant Physiology,2010,167(18):1571-1576.
[27]孟利,刘峰,付海燕,等.甜高粱秸秆中可溶性糖提取及组成分析[J].哈尔滨商业大学学报(自然科学版),2015,31(2):166-169.
[28]刘广平.绣球可溶性糖提取与测定方法研究[J].江苏农业科学,2012,40(4):253-255.
[29]胡文冉,范玲,马盾,等.棉纤维糖分不同提取方法的比较[J].新疆农业科学,2010,47(2):308-311.
[2]杨长琴,刘瑞显,张国伟,等.花铃期渍水对棉纤维糖代谢的影响及其与纤维比强度的关系[J].作物学报,2014,40(9):1612-1618.
[3]涂程,费魏鹤,籍晓萍,等.棉纤维含糖量测试方法的研究[J].中国纤检,2012(13):60-62.
[4]姚穆,杨建忠.棉花粘性与含糖的测试技术及发展趋势[J].西北纺织工学院学报,2001,15(2):198-200.[S]Donald,E.Brushwood.Modification of the potassium ferricyanide reducing sugar test for sugars from extracts of cotton fiber[J].The Journal of Cotton Science,2000(4):202-209.
[6]徐樽,丁泳,周新文,等.棉纤维含糖量分析测定的研究[J].塔里木农垦大学学报,1994,6(1):1-8.
[7]范玲,闰建庆,郑春霞,等.3,5-二硝基水杨酸(DNS)比色法在分析棉花纤维还原糖含量中的应用研究[J].棉花学报,1996,8(3):151-154.
[8]郭玉华,杨伟华,郁有祝,等.蒽酮比色法测定棉花成熟纤维中水溶性总糖含量[J].2011,48(12):23-26.
[9]周炜,李卫东,周兆懿,等.铁氰化钾法测棉花含糖量的影响因素研究[J].上海毛麻科技,2014(2):29-31.
[10]中华人民共和国国家质量监督检验检疫总局.进出口棉纤维含糖量检验方法第2部分色卡比色法:SN/T 0311.2-2010[S].北京:中国标准出版社,2010.
[11]中华人民共和国国家质量监督检验检疫总局.棉纤维含糖试验方法定量法:GB/T 16258—2008[S].北京:中国标准出版社,2008.
[12]刘萍,李明军.植物生理学实验技术[M].北京:科技出版社,2007:86-88.
[13]文赤夫,董爱文,李国章,等.蒽酮比色法测定紫花地丁中总糖及还原糖含量[J].现代食品科技,2005,21(3):122-123.
[14]刘海英,王华华,崔长海,等.可溶性糖含量测定(蒽酮法)实验的改进[J].实验室科学,2013,16(2):19-20.
[15]李晓旭,李家政.优化蒽酮比色法测定甜玉米中可溶性糖的含量[J].保鲜与加工,2013,13(4):24-27.
[16]丁雪梅,张晓君,赵云,等.蒽酮比色法测定可溶性糖含量的试验方法改进[J].实验室检测,2014(12):230-233.
[17]Ah Moradi,Omid Younesi.Influence of arbuscular mycorrhiza on membrane hpid peroxidation and soluble sugar content of soybean under salt stress[J].Agriculturae Conspectus Scientificus,2015,79(4):227-232.
[18]Nayer Mohammadkhani,Reza Heidari.Drought-induced accumulation of soluble sugars and proline in two maize varieties[J].World Apphed Sciences Journal,2008,3(3):448-453.
[19]郭晓蕾,朱思潮,翟旭峰,等.硫酸蒽酮法与硫酸苯酚法测定灵芝多糖含量比较[J].中华中医药学刊,2010,28(9):22-24.
[20]Marjorie R R,Patrick A J,Buddhi P L,et al.Total water soluble sugars quantification in bio-oil using the phenol sulfuric acid assay[J].Journal of Analytical and Apphed Pyrolysis,2013,104:194-201.
[21]祝义伟,周令国,叶宸志,等.香菇柄中可溶性糖的测定[J].农产品加工,2015(3):43-45.
[22]罗志刚,曾满枝,凌晨,等.3,5-二硝基水杨酸比色法测定烟草中水溶性总糖[J].中国烟草科学,2000(2):34-36.
[23]尹建雄,卢红,谢强,等.3,5-二硝基水杨酸比色法快速测定烟草水溶性总糖、还原糖及淀粉的探讨[J].云南农业大学学报,2007,22(6):829-833.
[24]郑荣庆.烟叶中还原糖的斐林试液比色测定法[J].烟草科技,1985(2):26-31.
[25]Liu H R Song H X,Liu D P,et al.Dynamics changes of soluble sugar and free amino acid contents in stem and leaf of different oilseed rape varieties[J].Acta Agriculturae Boreali-Occidentalis Sinica,2007,16(1):123-126.
[26]Kato-Noguchi Hisashi,Yasuda Yukihiro,Sasaki Ryosuke.Soluble sugar availability of aerobically germinated barley,oat and rice coleoptiles in anoxia[J].Journal of Plant Physiology,2010,167(18):1571-1576.
[27]孟利,刘峰,付海燕,等.甜高粱秸秆中可溶性糖提取及组成分析[J].哈尔滨商业大学学报(自然科学版),2015,31(2):166-169.
[28]刘广平.绣球可溶性糖提取与测定方法研究[J].江苏农业科学,2012,40(4):253-255.
[29]胡文冉,范玲,马盾,等.棉纤维糖分不同提取方法的比较[J].新疆农业科学,2010,47(2):308-311.
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