包埋辅酶Q10的纳米结构脂质载体的制备及性能研究

详细信息    查看官网全文

• 英文论文题名：Study of Preparation and Properties of CoQ10-Loaded Nanostructured Lipid Carriers
• 论文作者：吴丽娜 ; 梁蓉 ; 杨成
• 英文论文作者：Wu lina ; Liang Rong ; Yang Cheng ; School of Chemical and Material Engineering ; Jiangnan Univeristy ; The Key Laboratory of Food Colloids and Biotechnology ; Ministry of Education ; Jiangnan Univeristy
• 年：2016
• 作者机构：江南大学化学与材料工程学院;江南大学食品胶体与生物技术教育部重点实验室;
• 论文关键词：纳米结构脂质载体 ; 辅酶Q10 ; 制备 ; 体外透皮实验
• 英文论文关键词：Nanostructured Lipid Carriers(NLC) ; Coenzyme Q10 ; Preparation ; In-vitro Transdermal Study
• 会议召开时间：2016-06-15
• 会议录名称：第十一届中国化妆品学术研讨会论文集
• 英文会议录名称：The Proceedings of the 11th Seminar on Cosmetic China
• 语种：中文
• 分类号：TQ460.1;TQ658
• 学会代码：ZGXE
• 会议名称：第十一届中国化妆品学术研讨会
• 会议地点：中国上海
• 主办单位：中国香料香精化妆品工业协会
• 学会名称：中国香料香精化妆品工业协会
• 页数：10
• 文件大小：1341k
• 原文格式：O
• 会议级别：全国

摘要

本文采用高压均质法制备了运载辅酶Q10的纳米结构脂质载体(CoQ10-NLC),通过粒径、电位、包封率等测试手段对其制备条件进行优化,通过差示扫描热量仪(DSC)和透射电镜(TEM)对其结构和形貌进行表征,并通过体外模拟透皮实验考察了CoQ10-NLC的皮肤吸收效果。结果表明,CoQ10-NLC的最佳制备条件为:固液脂质的质量比为1:1,表面活性剂的质量分数为1.5%,高压均质压力为1000bar,循环次数为7次,并且制得的CoQ10-NLC能够增加CoQ10在皮肤中的渗透量。
In this study,CoQ10-loaded nanostructured lipid carriers(CoQ10-NLC) were prepared by high pressure homogenization method.The formulations were optimized by the determination of particle size,Zeta potential,encapsulation efficiency,ect.The structure and morphology of CoQ10-NLC were characterized by differential scanning calorimetry(DSC) and transmission electron microscopy(TEM).The skin absorption effect of CoQ10-NLC was assessed by in-vitro transdermal study.The optimized preparation conditions were that the mass ratio of solid and liquid lipid was 1:1,the mass fraction of surfactant was 1.5%,the preparation pressure was 1000 bar,the cycles were 7 times,and in-vitro transdermal study indicated CoQ10-NLC enhanced the permeation of CoQ10 in skin.

引文

[1]刘凯,孙进,何仲贵.新型纳米结构脂质载体系统的研究进展[J].沈阳药科大学学报,2008,25(3):236-242.
    [2]李静静,贾运涛,田睿,等.柚皮素纳米结构脂质载体的处方优化和初步评价[J].中草药,2015,46(2):211-215.
    [3]Pardeike J,Hommoss A,M(u|¨)ller RH.Lipid Nanoparticles(SLN,NLC)in Cosmetic and Pharmaceutical Dermal Products[J].International Journal of Pharmaceutics,2009,366(1):170-184.
    [4]H MR,D PR,A H,et al.Nanostructured Lipid Carriers(NLC)in Cosmetic Dermal Products.[J].Advanced Drug Delivery Reviews,2007,59(6):522-530.
    [5]Mitri K,Shegokar R,Gohla S,et al.Lipid Nanocarriers for Dermal Delivery of Lutein:Preparation,Characterization,Stability and Performance[J].International Journal of Pharmaceutics,2011,414(1):267-275.
    [6]冷福建.利多卡因固体脂质纳米粒的研究[D].武汉:华中科技大学,2012.
    [7]钱雪,王祖巧,韩国平,等.辅酶Q_(10)的药理与应用[J].食品与药品,2006,8(1):16-19.
    [8]薛建安,王可莉,魏敏吉.辅酶Q_(10)的抗氧化与抗疲劳作用及临床应用[J].中国医药导刊,2014,16(7):1167-1168.
    [9]Emrah K,H GE,Ozgen O.Development and Evaluation of Coenzyme Q10 Loaded Solid Lipid Nanoparticle Hydrogel for Enhanced Dermal Delivery.[J].Acta Pharmaceutica(zagreb,Croatia),2014,63(4):517-529.
    [10]段鹏杰,罗漾,姜同英,等.辅酶Q_(10)的溶解度测定及稳定生考察[J].中国药剂学杂志(网络版),2008,6(6):370-376.
    [11]黄丽君.高含量辅酶q10微囊的研究[D].济南:山东大学,2014.
    [12]夏书芹,许时婴.辅酶Q_(10)纳米脂质体的制备[J].食品工业科技,2006,27(2):164-168.
    [13]祝青哲.辅酶Q_(10)纳米脂质体的应用及辅酶Q_(10)前体脂质体的研究[D].无锡:江南大学,2008.
    [14]Farboud E,Nasrollahi S,Tabbakhi Z.Novel Formulation and Evaluation of a Q10-loaded Solid Lipid Nanoparticle Cream:in Vitro and in Vivo Studies.[J].International Journal of Nanomedicine,2011,(6):611-617.
    [15]Gao Y,Cheng X,Wang Z,et al.Transdermal Delivery of 10,11-methylenedioxycamptothecin By Hyaluronic Acid Based Nanoemulsion for Inhibition of Keloid fibroblast[J].Carbohydrate Polymers,2014,112(4):376-386.
    [16]Schwarz JC,Klang V,Karall S,et al.Optimisation of Multiple W/o/w Nanoemulsions for Dermal Delivery of Aciclovir[J].International Journal of Pharmaceutics,2012,435(1):69-75.
    [17]Justyna F,Marie-alexandrine B,Jocelyne P,et al.Topical Delivery of Lipophilic Drugs From O/w Pickering Emulsions.[J].International Journal of Pharmaceutics,2009,371(1):56-63.
    [18]Justyna F,Marie-alexandrine B,Jean-pierre V,et al.Pickering W/o Emulsions:Drug Release and Topical Delivery.[J].International Journal of Pharmaceutics,2008,368(1):7-15.
    [19]Andrade LM,Reis CDF,Maione-silva L,et al.Impact of Lipid Dynamic Behavior on Physical Stability,in Vitro Release and Skin Permeation of Genistein-loaded Lipid Nanoparticles[J].European Journal of Pharmaceutics and Biopharmaceutics,2014,(88):40-47.
    [20]Klang V,Schwarz JC,Lenobel B,et al.In Vitro Vs.in Vivo Tape Stripping:Validation of the Porcine Ear Model and Penetration Assessment of Novel Sucrose Stearate Emulsions[J].European Journal of Pharmaceutics and Biopharmaceutics,2012,80(3):604-614.
    [21]Combrinck J,Otto A,Plessis J.Whey Protein/polysaccharide-stabilized Emulsions:Effect of Polymer Type and Ph on Release and Topical Delivery of Salicylic Acid[J].Aaps Pharmscitech,2014,15(3):588-600.
    [22]阮婧华,肖吉娇,朱迪,等.口服槲皮素纳米结构脂质载体的制备及体外评价[J].华西药学杂志,2010,25(4):403-405.
    [23]Radheshyam T,Kamla P.Nanostructured Lipid Carrier Versus Solid Lipid Nanoparticles of Simvastatin:Comparative Analysis of Characteristics,Pharmacokinetics and Tissue Uptake.[J].International Journal of Pharmaceutics,2011,415(1):232-242.
    [24]ang X,Zhao L,Almasy L,et al.Preparation and Characterization of 4-dedimethylamino Sancycline(cmt-3)Loaded Nanostructured Lipid Carrier(cmt-3/NLC)Formulations[J].International Journal of Pharmaceutics,2013,450(1):225-234.
    [25]Ridolfi DM,Marcato PD,Justo GZ,et al.Chitosan-solid Lipid Nanoparticles as Carriers for Topical Delivery of Tretinoin[J].Colloids and Surfaces B:Biointerfaces,2012,93:36-40.
    [26]Teeranachaideekul V,Souto EB,Junyaprasert VB,et al.Cetyl Palmitate-based NLC for Topical Delivery of Coenzyme Q 10-Development,Physicochemical Characterization and in Vitro Release Studies[J].European Journal of Pharmaceutics and Biopharmaceutics,2007,67(1):141-148.
    [27]Jie L,Wen H,Huabing C,et al.Isotretinoin-loaded Solid Lipid Nanoparticles with Skin Targeting for opical Delivery.[J].International Journal of Pharmaceutics,2006,328(2):5-191.