细菌胁迫下刺参程序性细胞死亡因子4的克隆表达及功能分析

详细信息    查看官网全文

• 英文论文题名：Programmed cell death 4 in bacterially-challenged Apostichopus japonicus: Molecular cloning, expression analysis and functional characterization
• 论文作者：吕志猛 ; 李成华 ; 邵铱娜 ; 张卫卫 ; 王振辉 ; 王海宏
• 英文论文作者：LV Zhimeng ; LI Chenghua ; SHAO Yina ; ZHANG Weiwei ; WANG Zhenhui ; WANG Haihong ; School of Marine Sciences ; Ningbo University
• 年：2016
• 作者机构：海洋学院宁波大学;
• 论文关键词：刺参 ; 程序性死亡因子4 ; ROS产生 ; DNA损伤 ; p105
• 英文论文关键词：Apostichopus japonicus ; Programmed cell death 4 ; ROS production ; DNA damage ; p105
• 会议召开时间：2016-11-02
• 会议录名称：2016年中国水产学会学术年会论文摘要集
• 语种：中文
• 分类号：S917.4
• 学会代码：OGSB
• 会议名称：2016年中国水产学会学术年会
• 会议地点：中国四川成都
• 主办单位：中国水产学会、四川省水产学会
• 学会名称：中国水产学会
• 页数：1
• 文件大小：577k
• 原文格式：D
• 会议级别：全国

摘要

程序性细胞死亡因子4(PDCD4)在调节细胞信号过程中,尤其在TOLL信号通路参与的免疫反应中,起着至关重要的作用。在此研究中,通过RACE技术克隆获得一个新的刺参PDCD4同源基因(记为AjPDCD4)。AjPDCD4全长cDNA编码450个氨基酸残基,有两个典型的MA3结构域。系统发育树分析表明,AjPDCD4属于无脊椎动物PDCD4家族。组织表达分析表明AjPDCD4 mRNA在触手中高水平表达、肌肉细胞中低水平表达。灿烂弧菌的胁迫与LPS暴露可显著下调AjPDCD4 mRNA的表达。更重要的是,我们发现,紫外线(UV)可以诱导ROS的产生,AjPDCD4敲低后细胞水平的DNA损伤得到显著抑制。同时,在相同的条件下,NF-κB的同系物,p105表达上调。所有这些表明,AjPDCD4能是通过影响TLR信号通路,参与调节刺参DNA损伤和ROS的产生。
Programmed cell death 4(PDCD4) plays a crucial role in modulating cellular signals,mainly via TOLL cascades during the immune response. In the present study, a novel PDCD4 homologue gene(denoted as AjPDCD4) was cloned from the sea cucumber Apostichopus japonicus using RACE. The full-length AjPDCD4 cDNA encoded a 450 amino acid residue protein with two typical MA3 domains. Phylogenetic analysis revealed that AjPDCD4 belonged to the invertebrate PDCD4 family. Spatial expression analysis indicated that AjPDCD4 mRNA transcripts are expressed at a high level in the tentacles and at a low level in muscle compared with coelomocytes. Vibrio splendidus challenge and LPS exposure could both significantly down-regulate AjPDCD4 mRNA expression. More importantly, we found that ultraviolet(UV)-induced ROS production and DNA damage were greatly repressed in AjPDCD4-knockdown coelomocytes. Meanwhile, the expression levels of the NF-kappa B homologue, p105, were synchronously up-regulated in the same conditions. All of these results indicated that AjPDCD4 is involved in modulating DNA damage and ROS production in sea cucumber, perhaps by affecting the TLR pathway.

引文