摘要
2015年8月从辽宁盘锦、营口地区的26个发病的凡纳滨对虾养殖池塘中随机采集对虾样本,用PCR方法检测对虾传染性皮下与造血组织坏死病毒(IHHNV)、白斑综合征病毒(WSSV)和桃拉综合征病毒(TSV),用LAMP技术检测虾肝肠胞虫(EHP)的携带情况。结果显示,IHHNV和EHP的检出率较高,分别为65.38%和34.62%;WSSV和TSV的检出率较低,分别为19.23%和7.69%。EHP的检出与对虾大小密切相关,在生长缓慢对虾样本的检测阳性率高达100%。26份对虾样本中有7份样本同时携带EHP和IHHNV。结果表明,2015年8月该地区凡纳滨对虾携带的病原主要是IHHNV和EHP。
In order to investigate the infection rates of White Spot Syndrome Virus(WSSV),Infectious Hypodermal and Hematopoietic Necrosis Virus(IHHNV), Taura Syndrome Virus(TSV)and Enterocytozoon hepatopenaei(EHP) in diseased Litopenaeus vannamei farmed areas of Liaoning province in August 2015, the shrimp samples from Yingkou and Panjin aquaculture ponds were detected according to Polymerase Chain Reaction and Loop-mediated Isothermal Amplication. Detection results showed that IHHNV and EHP have a high infection rate, reached at65.38% and 34.62%, respectively. While the positive results of WSSV and TSV were 19.23% and7.69%, resepectively. All the small shrimp samples showed EHP positive. There were 7 samples were with the mixed infection of both EHP and IHHNV. All of the results showed that high positive rates of IHHNV and EHP were detected in this shrimp farmed area in August 2015.
引文