H9N2亚型禽流感病毒感染TC-1细胞诱导的免疫反应

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• 英文论文题名：Induced immune response of TC-1 cells in H9N2 avian influenza virus infection
• 论文作者：刘纪园 ; 孟丹 ; 黄蓉 ; 郝梦婵 ; 韦良孟 ; 柴同杰
• 英文论文作者：LIU Ji-yuan ; MENG Dan ; HUANG Rong ; HAO Meng-chan ; WEI Liang-meng ; CHAI Tongjie ; Sina-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province ; College of Animal Science and Veterinary Medicine ; Shandong Agricultural University ; Collaborative Innovation Centre for the Origin and Control of Emerging Infectious Diseases of Taishan Medical College ; College of Medical Test ; Xin Xiang Medical University
• 年：2016
• 作者机构：山东农业大学动物医学院山东省中德动物源人兽共患病合作研究中心;泰山医学院新发传染病溯源与控制协同创新中心;新乡医学院医学检验学院;
• 论文关键词：H9N2亚型禽流感病毒 ; TC-1细胞 ; 模式识别受体 ; 细胞因子 ; 趋化因子
• 英文论文关键词：H9N2 subtype avian influenza virus ; TC-1 cells ; pattern recognition receptors ; cytokines ; chemokines
• 会议召开时间：2016-11-04
• 会议录名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会论文集
• 语种：中文
• 分类号：S855.3
• 学会代码：ZGXJ
• 会议名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会
• 会议地点：中国山东青岛
• 主办单位：中国畜牧兽医学会动物微生态学分会
• 学会名称：中国畜牧兽医学会
• 页数：1
• 文件大小：81k
• 原文格式：O
• 会议级别：全国

摘要

为了更好地了解宿主机体对禽流感病毒感染后的免疫反应,本实验以TC-1细胞为模型,感染H9N2亚型禽流感病毒。通过荧光定量PCR(qRT-PCR)和酶联免疫吸附试验(ELISA)的方法检测了3种模式识别受体(TLR-3、RIG-I和MDA-5)和5种炎性细胞因子(IL-6、RANTES、IP-10、TNF-α和IFN-β)。结果显示:3种模式识别受体中MDA-5上调最为明显,5种炎性因子中RANTES和IP-10上调最为明显;而IL-6、TNF-α和IFN-β上调相对较平缓。本研究的结果表明:在病毒感染的过程中MDA-5的反应较TLR-3和RIG-I更为强烈,而在炎性反应的过程中RANTES和IP-10的反应更加剧烈,IL-6、TNF-α和IFN-β的反应则相对较温和一些。本研究在一定程度上反映了机体对流感病毒的免疫反应机制,为在生产中疫苗的使用具有一定的指导意义。
In order to know more about the immune response of the host organism to the infection of avian influenza virus,TC-1 cells were set as a model,which infected by H9N2 subtype AIV.3 kinds of pattern recognition receptors(TLR-3,RIG-I and MDA-5) and 5 kinds of inflammatory cytokines(IL-6,RANTES,IP-10,TNF-α and IFN-β) were detected by quantitative real-time polymerase chain reaction(qRT-PCR) and enzyme-linked immunosorbent assay(ELISA) methods.The results showed that:there were obvious up-regulation in MDA-5,RANTES and IP-10 among the 3 kinds of pattern recognition receptors and 5 kinds of inflammatory cytokines,while IL-6,TNF-α and IFN-β were just mild increase.The results of this study indicated that:MDA-5 played more important role in the identification of the virus TLR-3 and RIG-I,meanwhile,IP-10 and RANTES activated more intense reaction in the inflammatory reaction,the response of IL-6,TNF-αand IFN-β were relatively modest.To a certain extent,this study reflects the mechanism of the host immune response to influenza virus,and it has a certain guiding significance for the use of the vaccine in the production.

引文

[1]Alexander D J.A review of avian influenza in different bird species[J].Vet Microbiol,2000,74:3-13.
    [2]Peiris J S,Guan Y,Markwell D,et al.Cocirculation of avian H9N2 and contemporary"human"H3N2 influenza A viruses in pigs in southeastern China:potential for genetic reassortment?[J]J Virol,2001,75:9679-9686.