应用PCR-DGGE和Real-Time PCR分析健康与腹泻獭兔盲肠菌群

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• 英文论文题名：Application of PCR-DGGE and Real-Time PCR to analyze the cecum microflora of health and diarrhea Rex Rabbit
• 论文作者：周毅 ; 文斌 ; 孙豪 ; 傅祥超 ; 段玲 ; 曾东 ; 倪学勤 ; 汪平
• 英文论文作者：Zhou Yi ; Wen Bin ; Sun Hao ; Fu Xiang-chao ; Duan Ling ; Zeng Dong ; Ni Xue-qin ; Wang Ping ; College of Veterinary Medicine ; Sichuan Agricultural University ; Sichuan Academy of Grassland Science ; College of Life science & Technology ; Southwest University for Nationalities
• 年：2016
• 作者机构：四川农业大学动物医学院;四川省草原科学研究院;西南民族大学生命科学与技术学院;
• 论文关键词：腹泻 ; 肠道菌群 ; PCR-DGGE ; Real-Time PCR ; 獭兔
• 英文论文关键词：Diarrhea ; Intestinal microflora ; PCR-DGGE ; Real-Time PCR ; Rex Rabbit
• 会议召开时间：2016-11-04
• 会议录名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会论文集
• 语种：中文
• 分类号：S858.291
• 学会代码：ZGXJ
• 会议名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会
• 会议地点：中国山东青岛
• 主办单位：中国畜牧兽医学会动物微生态学分会
• 学会名称：中国畜牧兽医学会
• 页数：1
• 文件大小：90k
• 原文格式：O
• 会议级别：全国

摘要

研究獭兔腹泻发生后盲肠菌群结构的变化情况,为微生态防治断兔奶腹泻提供理论基础。分别提取5只健康和腹泻獭兔盲肠内容物总DNA,应用PCR-DGGE及Real-Time PCR技术比较分析獭兔盲肠中菌群结构的差异。腹泻獭兔PCR-DGGE图谱条带丰富度、均匀度、多样性指数与健康獭兔相比差异均不显著,但聚类分析和主成分分析(PCA)能够将腹泻组和健康组区分开。Real-Time PCR检测显示,腹泻獭兔盲肠正常肠道菌群普雷沃氏菌、拟球梭菌、梭菌类群I数量与健康獭兔相比没有变化(P>0.05);链球菌属、梭菌类群Ⅳ和ⅩⅣa、白色瘤胃球菌、溶纤维丁酸弧菌、普拉梭杆菌等有益微生物数量显著降低(P<0.01),而埃希氏大肠杆菌数量却显著升高(P<0.05)。结果表明,獭兔腹泻发生后盲肠有益微生物数量降低,有害微生物数量升高;菌群结构有差异但不显著(P>0.05),腹泻獭兔盲肠菌群结构有复杂化的趋势。
To provide theoretical basis for using probiotics to prevent and treat the diar-rhea weaning rabbit,the experime nts were performed to explore the structural changes of cecum m-icroflora in the diarrhea Rex rabbit.Total DNA were extracted from the cecum contents of 5 healthy and 5 diarrhea Rex Rabbits respectively.PCR-DGGE and Real-Time PCR were applied to analyze the differences of Cecum microflora between the healthy and diarrhea Rex Rabbits.Through the analysis we found that the richness.evenness and diversity index of PCR-DGGE profiles bands were not significant difference.But clustering analysis and Principal Component Analysis(PCA) could distinguish diarrhea group from healthy group.Real-Time PCR detection showed that in the diarrhea rabbit,despite the number of the population of normal intestinal flora including Prevotella Clostridium cluster I was not changed(P>0.01),the population of Streptococcus spp.Clostridium leptum group Bifidobacterium spp.Clostridium coccoides Ruminococcus albus Butyrivibrio flbrisolvens Fusobacterium rausnitzii were sharply decreased(P<0.01).However,the number of Escherichia coll which belongs to the Enterobacteriacae family,was greatly increased(P<0.05).The results prove that the population of probiotics decrease,some harmful microorganism quantity increase in the cecum of diarrhea Rex Rabbit;the cecum flora structure is difference but not significant(P>0.05),indeed the structure of flora has a tendency to complicate in the diarrhea rabbit.

引文

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