Identification and Characterization of Chlamydia abortion Isolates from Yaks in Qinghai,China
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- 英文论文题名：Identification and Characterization of Chlamydia abortion Isolates from Yaks in Qinghai,China
- 论文作者：Zhaocai Li ; Xiaoan Cao ; Baoquan Fu ; Yilin Chao ; Jinshan Cai ; Jizhang Zhou
- 英文论文作者：Zhaocai Li ; Xiaoan Cao ; Baoquan Fu ; Yilin Chao ; Jinshan Cai ; Jizhang Zhou ; State Key Laboratory of Veterinary Etiological Biology ; Lanzhou Veterinary Research Institute ; Chinese Academy of Agricultural Sciences ; Center for Animal Disease Control and Prevention in Qinghai Province
- 年：2016
- 作者机构：State Key Laboratory of Veterinary Etiological Biology,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences;Center for Animal Disease Control and Prevention in Qinghai Province;
- 会议召开时间：2016-07-20
- 会议录名称：中国畜牧兽医学会兽医公共卫生学分会第五次学术研讨会论文集
- 语种：英文
- 分类号：S858.23
- 学会代码：SYGW
- 会议名称：中国畜牧兽医学会兽医公共卫生学分会第五次学术研讨会
- 会议地点：中国内蒙古通辽
- 主办单位：中国畜牧兽医学会兽医公共卫生学分会
- 学会名称：中国畜牧兽医学会兽医公共卫生学分会
- 页数：11
- 文件大小：648k
- 原文格式：D
- 会议级别：全国
摘要
Chlamydia abortus(C.abortus) is an obligate intracellular bacterium responsible for producing disorders in domestic and wild mammals.Recent years,the yak(Bos grunniens)population demonstrated the problem of reproductive disorders,which is considered to be associated with C.abortus in Qinghai,China.Therefore,this study was conducted to identify C.abortus by PCR and culture in aborted yaks.During the birth season of 2012,a total of 9 aborted foetuses(each from a different herd) and 126 vaginal swab samples from the 9 herds were collected and analyzed.C.abortus DNA was detected from all of the 9 aborted foetuses and 30 of the 126 vaginal swab samples(23.81%) from yak cows in the selected herds.4 C.abortus strains were isolated from embryonated egg yolk sacs inoculated with foetal organ suspensions.In order to differentiate from C.psittaci and C.pecorum,the isolated C.abortus strains were further identified by PCR-based RFLP analysis.All isolates showed identical restriction profiles with the C.abortus reference strain using Alu I restriction enzyme in the RFLP test.Moreover,the isolated C.abortus strains and C.abortus-positive vaginal swab samples were genotyped by multi-loci variable number of tandem repeat analysis(MLVA) and all were belonged to the genotype 2 group.These findings suggested that C.abortus played a substantial role in yak abortion in Qinghai,China.
Chlamydia abortus(C.abortus) is an obligate intracellular bacterium responsible for producing disorders in domestic and wild mammals.Recent years,the yak(Bos grunniens)population demonstrated the problem of reproductive disorders,which is considered to be associated with C.abortus in Qinghai,China.Therefore,this study was conducted to identify C.abortus by PCR and culture in aborted yaks.During the birth season of 2012,a total of 9 aborted foetuses(each from a different herd) and 126 vaginal swab samples from the 9 herds were collected and analyzed.C.abortus DNA was detected from all of the 9 aborted foetuses and 30 of the 126 vaginal swab samples(23.81%) from yak cows in the selected herds.4 C.abortus strains were isolated from embryonated egg yolk sacs inoculated with foetal organ suspensions.In order to differentiate from C.psittaci and C.pecorum,the isolated C.abortus strains were further identified by PCR-based RFLP analysis.All isolates showed identical restriction profiles with the C.abortus reference strain using Alu I restriction enzyme in the RFLP test.Moreover,the isolated C.abortus strains and C.abortus-positive vaginal swab samples were genotyped by multi-loci variable number of tandem repeat analysis(MLVA) and all were belonged to the genotype 2 group.These findings suggested that C.abortus played a substantial role in yak abortion in Qinghai,China.
Chlamydia abortus(C.abortus) is an obligate intracellular bacterium responsible for producing disorders in domestic and wild mammals.Recent years,the yak(Bos grunniens)population demonstrated the problem of reproductive disorders,which is considered to be associated with C.abortus in Qinghai,China.Therefore,this study was conducted to identify C.abortus by PCR and culture in aborted yaks.During the birth season of 2012,a total of 9 aborted foetuses(each from a different herd) and 126 vaginal swab samples from the 9 herds were collected and analyzed.C.abortus DNA was detected from all of the 9 aborted foetuses and 30 of the 126 vaginal swab samples(23.81%) from yak cows in the selected herds.4 C.abortus strains were isolated from embryonated egg yolk sacs inoculated with foetal organ suspensions.In order to differentiate from C.psittaci and C.pecorum,the isolated C.abortus strains were further identified by PCR-based RFLP analysis.All isolates showed identical restriction profiles with the C.abortus reference strain using Alu I restriction enzyme in the RFLP test.Moreover,the isolated C.abortus strains and C.abortus-positive vaginal swab samples were genotyped by multi-loci variable number of tandem repeat analysis(MLVA) and all were belonged to the genotype 2 group.These findings suggested that C.abortus played a substantial role in yak abortion in Qinghai,China.
引文
[1]E.Campos-Hernandez,J.C.Vazquez-Chagoyan,A.Z.Salem,J.A.Saltijeral-Oaxaca,C.Escalante-Ochoa,S.M.Lopez-Heydeck,R.M.de Oca-Jimenez,“Prevalence and molecular identification of Chlamydia abortus in commercial dairy goat farms in a hot region in Mexico”,Tropical animal health and production,vol.46,no.6,pp.1-6,2014.
[2]H.Kalender,A.Kilic,H.Eroksuz,A.Muz,U.Kilinc,B.Tademir,“Identification of Chlamydophila abortus infection in aborting ewes and goats in Eastern Turkey”,Revue Med Vet,vol.164,no.6,pp.295-301,2013.
[3]N.Borel,R.Thoma,P.Spaeni,R.Weilenmann,K.Teankum,E.Brugnera,D.Zimmermann,L.Vaughan,A.Pospischil,“Chlamydia-related abortions in cattle from Graubunden,Switzerland”.Veterinary Pathology Online,vol.43,no.5,pp.702-708,2006.
[4]K.D.Everett,R.M.Bush,A.A.Andersen,“Emended description of the order Chlamydiales,proposal of Parachlamydiaceae fam.nov.and Simkaniaceae fam.nov.,each containing one monotypic genus,revised taxonomy of the family Chlamydiaceae,including a new genus and five new species,and standards for the identification of organisms”,International Journal of Systematic Bacteriology,vol.49,no.2,pp.415-440,1999.
[5]A.Pospischil,R.Thoma,M.Hilbe,P.Grest,J.O.Gebbers,“Abortion in woman caused by caprine Chlamydophila abortus(Chlamydia psittaci serovar 1)”,Swiss Med Wkly,vol.132,no.5-6,pp.64-66,2002.
[6]G.Walder,H.Hotzel,C.Brezinka,W.Gritsch,R.Tauber,R.Würzner,F.Ploner,“An unusual cause of sepsis during pregnancy:recognizing infection with Chlamydophila abortus”,Obstetrics&Gynecology,vol.106,no.5,pp.1215-1217,2005.
[7]M.Sait,E.M.Clark,N.Wheelhouse,L.Spalding,M.Livingstone,K.Sachse,B.K.Markey,S.Magnino,V.I.Siarkou,E.Vretou,“Genetic variability of Chlamydophila abortus strains assessed by PCR-RFLP analysis of polymorphic membrane protein-encoding genes”,Veterinary microbiology,vol.151,no.3,pp.284-290,2011.
[8]K.Laroucau,F.Vorimore,C.Bertin,K.Y.Mohamad,S.Thierry,W.Hermann,C.Maingourd,C.Pourcel,D.Longbottom,S.Magnino,K.Sachse,E.Vretou,A.Rodolakis,“Genotyping of Chlamydophila abortus strains by multilocus VNTR analysis”,Vet Microbiol,vol.137,no.3-4,pp.335-344,2009.
[9]M.Li,“A diagnosis study of brucellosis and Chlamydia infection in yak”,Proceedings of the third international congress on yak,in Lhasa,China,International Livestock Research Institute(ILRI),Nairobi,pp.463-464,2000.
[10]S.Bandyopadhyay,D.Sasmal,T.Biswas,I.Samanta,M.Ghosh,“Serological evidence of antibodies against Chlamydophila abortus in free-ranging yak(Poephagus grunniens)in Arunachal Pradesh,India”,Revue scientifique et technique(International Office of Epizootics),vol.28,no.3,pp.1051-1055,2009.
[11]Q.Chen,X.Gong,F.Zheng,X.Cao,Z.Li,J.Zhou,“Seroprevalence of Chlamydophila abortus infection in yaks(Bos grunniens)in Qinghai,China”,Tropical animal health and production,vol.46,no.3,pp.503-507,2014.
[12]H.Geilhausen,“Serological survey on infectious diseases of a white yak herd in the Gansu Province”,In:Proceedings of the 3rd International congress on yaks held in Lhasa(September 4-9 2000)Nairobi,Kenya:2000;2000.
[13]K.Laroucau,A.Souriau,A.Rodolakis,“Improved sensitivity of PCR for Chlamydophila using pmp genes”,Veterinary Microbiology,vol.82,no.2,pp.155-164,2001.
[14]J.Hartley,S.Kaye,S.Stevenson,J.Bennett,G.Ridgway,“PCR Detection and Molecular Identification of Chlamydiaceae Species”,Journal of clinical microbiology,vol.39,no.9,pp.3072-3079,2001.
[15]J.L Creelan,S.J.Mc Cullough,“Evaluation of strain‐specific primer sequences from an abortifacient strain of ovine Chlamydophila abortus(Chlamydia psittaci)for the detection of EAE by PCR”,FEMS microbiology letters,vol.190,no.1,pp.103-108,2000.
[16]C.Qiu,J.Zhou,S.Cheng,X.Cao,S.Gao,W.Jia,“Development of inactivated vaccine against chlamydiosis in dairy cattle”,Veterinary Science in China,vol.36,no.5,pp.343-34,2006.
[17]C.Qiu,J.Zhou,X.Cao,G.Lin,F.Zheng,X.Gong.“Immunization trials with an avian chlamydial MOMP gene recombinant adenovirus”,Bioeng Bugs,vol.1,no.4,pp.267-273,2010.
[18]K.Laroucau,S.Thierry,F.Vorimore,K.Blanco,E.Kaleta,R.Hoop,S.Magnino,D.Vanrompay,K.Sachse,G.S.Myers,“High resolution typing of Chlamydophila psittaci by multilocus VNTR analysis(MLVA)”,Infection,Genetics and Evolution,vol.8,no.2,pp.171-181,2008.
[19]X.Lang,H.Qu,Z.Bu,Y.Yang,C.Sun,X.Li,J.Wang,J.Cai,R.Ma,Y.Fu,X.Wang,“Seroprevalence of Brucella infection in yaks(Bos grunniens)on the Qinghai-Tibet plateau of China”,Tropical animal health and production,vol.43,no.2,pp.305-306,2011.
[20]Q.Liu,J.Cai,Q.Zhao,L.Shang,R.Ma,X.Wang,J.Li,G.Hu,H.Jin,H.Gao,“Seroprevalence of Toxoplasma gondii infection in yaks(Bos grunniens)in northwestern China”,Tropical animal health and production,vol.43,no.4,pp.741-743,2011.
[21]J.Gao,M.Liu,X.Meng,Z.Han,D.Zhang,B.Hou,K.Zhang,S.SIzhu,J.Li,“Seroprevalence of bovine viral diarrhea infection in Yaks(Bos grunniens)on the Qinghai-Tibetan Plateau of China”,Tropical animal health and production,vol.45,no.3,pp.791-793,2013.
[22]Q.Zhu,Y.Liu,“Seroprevalence of Chlamydia infection in yask in Delingha,Qinghai”,The Chinese Livestock and Poultry Breeding,vol.29,no.12,pp.41-42,2012.
[23]L.Zhang,X.Wang,M.Zhang,H.La,X.You,“Seroprevalence investigation of Chlamydia in livestocks in Qinghai Province”,Chinese Journal of Veterinary Medicine,vol.50,no.4,pp.38-39,2014.
[24]Y.Shuai,Y.Li,K.Dan,Y.Duan,G.Cheng,Z.Yang Ziping,X.Yang,Y.Diao,G.Ygu,X.Li,Y.Zhao,Q.Ga,H.Jiao,“Diagnosis of enzootic abortion in yak”,Scientia Agricultura Sinica,vol.21,no.4,pp.76-81,1988.
[25]K.D.Everett,“Chlamydia and Chlamydiales:more than meets the eye”,Veterinary microbiology,vol.75,no.2,pp.109-126,2000.
[26]A.Rodolakis,A.Souriau,“Variations in the virulence of strains of Chlamydia psittaci for pregnant ewes”,The Veterinary record,vol.125,no.4,pp.87-90,1989.
[27]H.Ongor,B.Cetinkaya,M.Acik,M.Karahan,H.Bulut,“Detection of Chlamydophila abortus in Ovine Milk by Immunomagnetic Separation-Polymerase Chain Reaction”,Journal of Veterinary Medicine,Series B,vol.51,no.1,pp.43-45,2004.
[28]S.Wu,S.Huang,M.Xu,D.Zhou,H.Song,X.Zhu,“Chlamydia felis exposure in companion dogs and cats in Lanzhou,China:a public health concern”,BMC veterinary research,vol.104,no.9,pp.1-5,2013.
[29]A.Rodolakis,F.Bernard,Vaccination with temperature-sensitive mutant of Chlamydia psittaci against enzootic abortion of ewes,Veterinary Record,vol.114,no.8,pp.193-194,1984.
[30]W.Cong,S.Huang,X.Zhang,D.Zhou,M.Xu,Q.Zhao,H.Song,X.Zhu,A.Qian,“Seroprevalence of Chlamydia psittaci infection in market-sold adult chickens,ducks and pigeons in north-western China”,Journal of medical microbiology,vol.62,no,Pt 8,pp.1211-1214,2013.
[31]K.Dan,Y.Shuai,Y.Li,X.Yang,Y.Duan,S.Gao,G.Cheng,X.Yang,“Field test of inactivated vaccine against chlamydiosis in sheep”,Chinese Journal of Preventive Veterinary,vol.13,no.2,pp.6-8,1991.
[32]V.Siarkou,A.F.Lambropoulos,S.Chrisafi,A.Kotsis,O.Papadopoulos,“Subspecies variation in Greek strains of Chlamydophila abortus”,Veterinary Microbiology,vol.85,no.2,pp.145-157,2002.
[33]A.Bouakane,A.Rekiki,A.Rodolakis,“Protection of pregnant mice against placental and splenic infection by three strains of Chlamydophila abortus with a live 1B vaccine”,Veterinary Record,vol.157,no.24,pp.771-774,2005.
[2]H.Kalender,A.Kilic,H.Eroksuz,A.Muz,U.Kilinc,B.Tademir,“Identification of Chlamydophila abortus infection in aborting ewes and goats in Eastern Turkey”,Revue Med Vet,vol.164,no.6,pp.295-301,2013.
[3]N.Borel,R.Thoma,P.Spaeni,R.Weilenmann,K.Teankum,E.Brugnera,D.Zimmermann,L.Vaughan,A.Pospischil,“Chlamydia-related abortions in cattle from Graubunden,Switzerland”.Veterinary Pathology Online,vol.43,no.5,pp.702-708,2006.
[4]K.D.Everett,R.M.Bush,A.A.Andersen,“Emended description of the order Chlamydiales,proposal of Parachlamydiaceae fam.nov.and Simkaniaceae fam.nov.,each containing one monotypic genus,revised taxonomy of the family Chlamydiaceae,including a new genus and five new species,and standards for the identification of organisms”,International Journal of Systematic Bacteriology,vol.49,no.2,pp.415-440,1999.
[5]A.Pospischil,R.Thoma,M.Hilbe,P.Grest,J.O.Gebbers,“Abortion in woman caused by caprine Chlamydophila abortus(Chlamydia psittaci serovar 1)”,Swiss Med Wkly,vol.132,no.5-6,pp.64-66,2002.
[6]G.Walder,H.Hotzel,C.Brezinka,W.Gritsch,R.Tauber,R.Würzner,F.Ploner,“An unusual cause of sepsis during pregnancy:recognizing infection with Chlamydophila abortus”,Obstetrics&Gynecology,vol.106,no.5,pp.1215-1217,2005.
[7]M.Sait,E.M.Clark,N.Wheelhouse,L.Spalding,M.Livingstone,K.Sachse,B.K.Markey,S.Magnino,V.I.Siarkou,E.Vretou,“Genetic variability of Chlamydophila abortus strains assessed by PCR-RFLP analysis of polymorphic membrane protein-encoding genes”,Veterinary microbiology,vol.151,no.3,pp.284-290,2011.
[8]K.Laroucau,F.Vorimore,C.Bertin,K.Y.Mohamad,S.Thierry,W.Hermann,C.Maingourd,C.Pourcel,D.Longbottom,S.Magnino,K.Sachse,E.Vretou,A.Rodolakis,“Genotyping of Chlamydophila abortus strains by multilocus VNTR analysis”,Vet Microbiol,vol.137,no.3-4,pp.335-344,2009.
[9]M.Li,“A diagnosis study of brucellosis and Chlamydia infection in yak”,Proceedings of the third international congress on yak,in Lhasa,China,International Livestock Research Institute(ILRI),Nairobi,pp.463-464,2000.
[10]S.Bandyopadhyay,D.Sasmal,T.Biswas,I.Samanta,M.Ghosh,“Serological evidence of antibodies against Chlamydophila abortus in free-ranging yak(Poephagus grunniens)in Arunachal Pradesh,India”,Revue scientifique et technique(International Office of Epizootics),vol.28,no.3,pp.1051-1055,2009.
[11]Q.Chen,X.Gong,F.Zheng,X.Cao,Z.Li,J.Zhou,“Seroprevalence of Chlamydophila abortus infection in yaks(Bos grunniens)in Qinghai,China”,Tropical animal health and production,vol.46,no.3,pp.503-507,2014.
[12]H.Geilhausen,“Serological survey on infectious diseases of a white yak herd in the Gansu Province”,In:Proceedings of the 3rd International congress on yaks held in Lhasa(September 4-9 2000)Nairobi,Kenya:2000;2000.
[13]K.Laroucau,A.Souriau,A.Rodolakis,“Improved sensitivity of PCR for Chlamydophila using pmp genes”,Veterinary Microbiology,vol.82,no.2,pp.155-164,2001.
[14]J.Hartley,S.Kaye,S.Stevenson,J.Bennett,G.Ridgway,“PCR Detection and Molecular Identification of Chlamydiaceae Species”,Journal of clinical microbiology,vol.39,no.9,pp.3072-3079,2001.
[15]J.L Creelan,S.J.Mc Cullough,“Evaluation of strain‐specific primer sequences from an abortifacient strain of ovine Chlamydophila abortus(Chlamydia psittaci)for the detection of EAE by PCR”,FEMS microbiology letters,vol.190,no.1,pp.103-108,2000.
[16]C.Qiu,J.Zhou,S.Cheng,X.Cao,S.Gao,W.Jia,“Development of inactivated vaccine against chlamydiosis in dairy cattle”,Veterinary Science in China,vol.36,no.5,pp.343-34,2006.
[17]C.Qiu,J.Zhou,X.Cao,G.Lin,F.Zheng,X.Gong.“Immunization trials with an avian chlamydial MOMP gene recombinant adenovirus”,Bioeng Bugs,vol.1,no.4,pp.267-273,2010.
[18]K.Laroucau,S.Thierry,F.Vorimore,K.Blanco,E.Kaleta,R.Hoop,S.Magnino,D.Vanrompay,K.Sachse,G.S.Myers,“High resolution typing of Chlamydophila psittaci by multilocus VNTR analysis(MLVA)”,Infection,Genetics and Evolution,vol.8,no.2,pp.171-181,2008.
[19]X.Lang,H.Qu,Z.Bu,Y.Yang,C.Sun,X.Li,J.Wang,J.Cai,R.Ma,Y.Fu,X.Wang,“Seroprevalence of Brucella infection in yaks(Bos grunniens)on the Qinghai-Tibet plateau of China”,Tropical animal health and production,vol.43,no.2,pp.305-306,2011.
[20]Q.Liu,J.Cai,Q.Zhao,L.Shang,R.Ma,X.Wang,J.Li,G.Hu,H.Jin,H.Gao,“Seroprevalence of Toxoplasma gondii infection in yaks(Bos grunniens)in northwestern China”,Tropical animal health and production,vol.43,no.4,pp.741-743,2011.
[21]J.Gao,M.Liu,X.Meng,Z.Han,D.Zhang,B.Hou,K.Zhang,S.SIzhu,J.Li,“Seroprevalence of bovine viral diarrhea infection in Yaks(Bos grunniens)on the Qinghai-Tibetan Plateau of China”,Tropical animal health and production,vol.45,no.3,pp.791-793,2013.
[22]Q.Zhu,Y.Liu,“Seroprevalence of Chlamydia infection in yask in Delingha,Qinghai”,The Chinese Livestock and Poultry Breeding,vol.29,no.12,pp.41-42,2012.
[23]L.Zhang,X.Wang,M.Zhang,H.La,X.You,“Seroprevalence investigation of Chlamydia in livestocks in Qinghai Province”,Chinese Journal of Veterinary Medicine,vol.50,no.4,pp.38-39,2014.
[24]Y.Shuai,Y.Li,K.Dan,Y.Duan,G.Cheng,Z.Yang Ziping,X.Yang,Y.Diao,G.Ygu,X.Li,Y.Zhao,Q.Ga,H.Jiao,“Diagnosis of enzootic abortion in yak”,Scientia Agricultura Sinica,vol.21,no.4,pp.76-81,1988.
[25]K.D.Everett,“Chlamydia and Chlamydiales:more than meets the eye”,Veterinary microbiology,vol.75,no.2,pp.109-126,2000.
[26]A.Rodolakis,A.Souriau,“Variations in the virulence of strains of Chlamydia psittaci for pregnant ewes”,The Veterinary record,vol.125,no.4,pp.87-90,1989.
[27]H.Ongor,B.Cetinkaya,M.Acik,M.Karahan,H.Bulut,“Detection of Chlamydophila abortus in Ovine Milk by Immunomagnetic Separation-Polymerase Chain Reaction”,Journal of Veterinary Medicine,Series B,vol.51,no.1,pp.43-45,2004.
[28]S.Wu,S.Huang,M.Xu,D.Zhou,H.Song,X.Zhu,“Chlamydia felis exposure in companion dogs and cats in Lanzhou,China:a public health concern”,BMC veterinary research,vol.104,no.9,pp.1-5,2013.
[29]A.Rodolakis,F.Bernard,Vaccination with temperature-sensitive mutant of Chlamydia psittaci against enzootic abortion of ewes,Veterinary Record,vol.114,no.8,pp.193-194,1984.
[30]W.Cong,S.Huang,X.Zhang,D.Zhou,M.Xu,Q.Zhao,H.Song,X.Zhu,A.Qian,“Seroprevalence of Chlamydia psittaci infection in market-sold adult chickens,ducks and pigeons in north-western China”,Journal of medical microbiology,vol.62,no,Pt 8,pp.1211-1214,2013.
[31]K.Dan,Y.Shuai,Y.Li,X.Yang,Y.Duan,S.Gao,G.Cheng,X.Yang,“Field test of inactivated vaccine against chlamydiosis in sheep”,Chinese Journal of Preventive Veterinary,vol.13,no.2,pp.6-8,1991.
[32]V.Siarkou,A.F.Lambropoulos,S.Chrisafi,A.Kotsis,O.Papadopoulos,“Subspecies variation in Greek strains of Chlamydophila abortus”,Veterinary Microbiology,vol.85,no.2,pp.145-157,2002.
[33]A.Bouakane,A.Rekiki,A.Rodolakis,“Protection of pregnant mice against placental and splenic infection by three strains of Chlamydophila abortus with a live 1B vaccine”,Veterinary Record,vol.157,no.24,pp.771-774,2005.
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