鸭短喙-侏儒综合征及其病原的初步研究

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• 英文论文题名：Case study on duck short beak and dwarfism syndrome
• 论文作者：崔元 ; 王建昌 ; 李玉保 ; 经美 ; 张姗 ; 陈萍 ; 孙继国 ; 袁万哲
• 英文论文作者：CUI Yuan ; WANG Jianchang ; LI Yubao ; JING Mei ; ZHANG Shan ; Chen Ping ; SUN Ji-guo ; YUAN Wan-zhe ; College of Animal Medicine ; Agricultural University of Hebei ; Agricultural College ; Liaocheng University ; Inspection and Quarantine Technical Center of Hebei Entry-Exit Inspection and Quarantine Bureau
• 年：2016
• 作者机构：河北农业大学动物医学院;河北出入境检验检疫局检验检疫技术中心;聊城大学农学院;
• 论文关键词：鸭短喙-侏儒综合征 ; 细小病毒 ; PCR
• 英文论文关键词：Beak atrophy and dwarfism syndrome(BADS) ; Parvovirus ; PCR
• 会议召开时间：2016-07-20
• 会议录名称：中国畜牧兽医学会兽医公共卫生学分会第五次学术研讨会论文集
• 语种：中文
• 分类号：S858.32
• 学会代码：SYGW
• 会议名称：中国畜牧兽医学会兽医公共卫生学分会第五次学术研讨会
• 会议地点：中国内蒙古通辽
• 主办单位：中国畜牧兽医学会兽医公共卫生学分会
• 学会名称：中国畜牧兽医学会兽医公共卫生学分会
• 页数：6
• 文件大小：654k
• 原文格式：D
• 会议级别：全国

摘要

2014年11月以来,我国部分地区所饲养的肉鸭发生了以雏鸭发育迟缓,上下喙萎缩,舌头外伸为特征的疾病。根据病变,暂将该病称为鸭短喙-侏儒综合征。本研究从山东聊城发病鸭群采集14份病料样品,其中包括舌及各脏器组织样品。经PCR方法检测,所有发病鸭肝、脾中均可检出细小病毒,将其中5份阳性病料处理后接种9日龄鸭胚,分离得到1株鸭细小病毒分离株(命名为SD)。此外,该分离株的659bp的Rep和VP1核苷酸序列与鹅细小病毒和番鸭细小病毒进行分析表明,SD株与鹅细小病毒具有相近的遗传进化关系。将分离株人工感染1日龄樱桃谷肉鸭,可复制出相似病例。结果表明,从鸭短喙-侏儒综合征自然病例分离到的一种鸭源鹅细小病毒可能与鸭短喙-侏儒综合征有关。
Since November in 2014,an infectious disease has emerged in many duck farms in some regions of China,which characterized by serious growth retardation and short bills with protruding tongues.Based on the pathological changes,the disease was tentatively designated beak atrophy and dwarfism syndrome(BADS).In this study,fourteen samples including tongue and organ tissues were collected from the diseased ducks in Liao Cheng,Shandong province,and all liver and spleen samples were duck parvovirus positive detected by PCR.In addition,a virus strain was isolated from spleen tissue sample with 9-day-old duck embryos,and PCR results showed that the isolate was positive for duck parvovirus,designated SD.Sequence analysis based on a 659 bp fragment of the virus DNA,partial of Rep and VP3 gene sequence of SD strain revealed that the isolate was most closely related to goose parvovirus.Moreover,the clinical signs of BADS were able to be reproduced in one-day-old Cherry Valley Peking ducks by artificial infection with the isolate of a duck parvovirus.These evidences indicated that the SD strain was isolated from ducks with BADS,which might be caused by duck parvovirus infection.

引文

[1]Villatte,D.Maladie de Derzsy ou hepatonephrite-ascite de l’oison et du caneton de Barbarie(h.n.a.)ou parvovirose[J].In Manuel pratique des maladies des palmipedes,1989,1:114-117.
    [2]Samorek-Salamonowicz E,Budzyk J,Tomczyk G.Syndrom karlowatosci i skroconego dzioba u kaczek mulard[J].?ycie Weterynaryjne,1995,2(70):56-7.
    [3]Palya V,Zolnai A,Benyeda Z,et al.Short beak and dwarfism syndrome of mule duck is caused by a distinct lineage of goose parvovirus[J].Avian Pathology,2009,38(2):175-180.
    [4]Tatar-Kis T,Mato T,Markos B,Palya V.Phylogenetic analysis of Hungarian goose parvovirus isolates and vaccine strains[J].Avian pathology:journal of the WVPA,2004;33(4):438-44.
    [5]Lu,Y.S.,Lin,D.F.,Lee,Y.L,et,al.Infectious bill atrophy syndrome caused by parvovirus in a co-outbreak with duck viral hepatitis in duckings in Taiwan[J].Avian Diseases,1993,37:591-596.
    [6]宁康,王丹,王府民等.樱桃谷北京鸭短喙和侏儒综合征的初步研究[J].中国兽医学报,2015,10(51):7~10
    [7]马磊,董浩,蒋运博等.鹅细小病毒研究进展[J].中国畜牧兽医,2011(6):165-168.
    [8]Chen Hao,Dou Yan-guo,Tang Yi,et al.Isolation and Genomic Characterization of a Duck-Origin GPV-Related Parvovirus from Cherry Valley Ducklings in China[J].PLo SONE,2015,10(10):e0140284
    [9]徐浩.鹅细小病毒的分离鉴定及其全基因测序及结构蛋白的表达[D].吉林农业大学动物科学技术学院,2014,4.
    [10]王欣.番鸭细小病毒的分离鉴定、vp基因的克隆及其原核表达[D].西南大学,2008,5.