RNA-Seq reveals the molecular mechanism of stipe elongation in the straw mushroom Volvariella volvacea

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• 英文论文题名：RNA-Seq reveals the molecular mechanism of stipe elongation in the straw mushroom Volvariella volvacea
• 论文作者：Yongxin Tao ; Arend F.van Peer ; Yuji Jiang ; Jian Zhu ; Baogui Xie
• 英文论文作者：Yongxin Tao ; Arend F.van Peer ; Yuji Jiang ; Jian Zhu ; Baogui Xie ; Mycological Research Center ; Fujian Agriculture and Forestry University
• 年：2016
• 作者机构：Mycological Research Center,Fujian Agriculture and Forestry University;
• 英文论文关键词：Volvariella volvacea ; Stipe elongation ; Cell division ; Cell wall degradation enzymes
• 会议召开时间：2016-08-19
• 会议录名称：中国菌物学会2016年学术年会论文摘要集
• 英文会议录名称：Abstracts of 2016 Mycological Society of China Annual Meeting
• 语种：英文
• 分类号：S646.13
• 学会代码：ZGVL
• 会议名称：中国菌物学会2016年学术年会
• 会议地点：中国福建福州
• 主办单位：中国菌物学会
• 学会名称：中国菌物学会
• 页数：1
• 文件大小：803k
• 原文格式：D
• 会议级别：全国

摘要

The edible straw mushroom Volvariella volvacea is cultivated on a large scale for its unique flavor and nutritious value.Its characteristic five-step stages:primordia(PR),button stage(BU),egg stage(EG),elongation stage(EL) and maturation stage(MA),make it a suitable model for studies on fruiting body development.One of the main factors that negatively affect yield and value is the rapid transition from EG to EL.To understand deep the stipe development,we analyzed genes differential expression in stipe tissue of V.volvacea using RNA-Seq.Stipe development turned out to be fairly complex,and regulation of stage differences is mediated mainly by changes in expression levels of genes,rather than on/off modulation.Most explicit is the strong up-regulation of cell division from BU to EG,and the very strong down-regulation hereof from EG to EL,that continues in the MA.BU and EG share cell division as means of growth,followed by a major developmental shift towards the EL with inactivation of cell division.Examination of regulatory genes up-regulated from EG to EL,identified three potential high upstream regulators for this switch.Therefore,V.volvacea stipe elongation was the result of vertical elongation of stipe cell,not the cell mitosis.In addition,stipe elongation is related to reconstruction of stipe cell walls.Total sixteen carbohydrate-active enzymes up-regulated during stipe elongation were identified.Their specific expression pattern and molecular functions hinted that they could hydrolyze glucan or chitin of cell wall to participate in the reconstruction of cell wall as the ‘final operators' during the rapid stipe elongation.In conclusion,the new insights in stipe dynamics,together with a series of new target genes involved in stipe elongation,helps to construct a draft model of molecular mechanism of V.volvacea stipe elongation.
The edible straw mushroom Volvariella volvacea is cultivated on a large scale for its unique flavor and nutritious value.Its characteristic five-step stages:primordia(PR),button stage(BU),egg stage(EG),elongation stage(EL) and maturation stage(MA),make it a suitable model for studies on fruiting body development.One of the main factors that negatively affect yield and value is the rapid transition from EG to EL.To understand deep the stipe development,we analyzed genes differential expression in stipe tissue of V.volvacea using RNA-Seq.Stipe development turned out to be fairly complex,and regulation of stage differences is mediated mainly by changes in expression levels of genes,rather than on/off modulation.Most explicit is the strong up-regulation of cell division from BU to EG,and the very strong down-regulation hereof from EG to EL,that continues in the MA.BU and EG share cell division as means of growth,followed by a major developmental shift towards the EL with inactivation of cell division.Examination of regulatory genes up-regulated from EG to EL,identified three potential high upstream regulators for this switch.Therefore,V.volvacea stipe elongation was the result of vertical elongation of stipe cell,not the cell mitosis.In addition,stipe elongation is related to reconstruction of stipe cell walls.Total sixteen carbohydrate-active enzymes up-regulated during stipe elongation were identified.Their specific expression pattern and molecular functions hinted that they could hydrolyze glucan or chitin of cell wall to participate in the reconstruction of cell wall as the ‘final operators' during the rapid stipe elongation.In conclusion,the new insights in stipe dynamics,together with a series of new target genes involved in stipe elongation,helps to construct a draft model of molecular mechanism of V.volvacea stipe elongation.

引文

Kamada T,Takemaru T(1977)Stipe elongation during basidiocarp maturation in Coprinus macrorhizus:mechanical properties of stipe cell wall.Plant Cell Physiol 18:831-840.
    Muraguchi H,Kamada T(2000)A mutation in the eln2 gene encoding a cytochrome P450 of Coprinus cinereus affects mushroom morphogenesis.Fungal Genet Biol 29:49-59.
    Shioya T,Nakamura H,Ishii N,Takahashi N,Sakamoto Y,et al.(2013)The Coprinopsis cinerea septin Cc.Cdc3 is involved in stipe cell elongation.Fungal Genet Biol 58-59:80-90.