MAP4K4 deficiency in CD4~+ T cells aggravates lung damage induced by ozone-oxidized black carbon particles

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• 英文论文题名：MAP4K4 deficiency in CD4~+ T cells aggravates lung damage induced by ozone-oxidized black carbon particles
• 论文作者：Ming Jin ; Hongqian Chu ; Yuan Li ; Xi Tao ; Zhiyuan Cheng ; Yao Pan ; Qinghe Meng ; Leilei Li ; Xiaohong Hou ; Yueyue Chen ; Hongpeng Huang ; Guang jia ; Jing Shang ; Tong Zhu ; Lanqin Shang ; Weidong Hao ; Xuetao Wei
• 英文论文作者：Ming Jin ; Hongqian Chu ; Yuan Li ; Xi Tao ; Zhiyuan Cheng ; Yao Pan ; Qinghe Meng ; Leilei Li ; Xiaohong Hou ; Yueyue Chen ; Hongpeng Huang ; Guang jia ; Jing Shang ; Tong Zhu ; Lanqin Shang ; Weidong Hao ; Xuetao Wei ; Department of Toxicology ; School of Public Health ; Peking University ; Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety ; State Key Joint Laboratory of Environmental Simulation and Pollution Control ; College of Environmental Sciences and Engineering ; Peking University ; Department of Occupational and Environmental Health Sciences ; School of Public Health ; Peking University
• 年：2016
• 作者机构：Department of Toxicology,School of Public Health,Peking University;Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety;State Key Joint Laboratory of Environmental Simulation and Pollution Control,College of Environmental Sciences and Engineering,Peking University;Department of Occupational and Environmental Health Sciences,School of Public Health,Peking University;
• 英文论文关键词：ozone-oxidized black carbon ; MAP4K4 ; CD4~+ T cell ; CD4~+IL17~+ T cell ; lung inflammation
• 会议召开时间：2016-11-16
• 会议录名称：第六届中国毒理学会免疫毒理专业委员会换届及学术会议资料汇编
• 语种：英文
• 分类号：R563
• 学会代码：ZGDV
• 会议名称：第六届中国毒理学会免疫毒理专业委员会换届及学术会议
• 会议地点：中国河南开封
• 主办单位：中国毒理学会免疫毒理专业委员会
• 学会名称：中国毒理学会
• 页数：12
• 文件大小：2690k
• 原文格式：O
• 会议级别：全国

摘要

As the main composition of combustion,black carbon(BC) is becoming more and more noticeable at home and abroad.Ozone-oxidized black carbon(oBC)was produced through aging of ozone,one of the near-surface pollutants,to black carbon.And oBC was found to be more oxidation and cell toxicity when compared with BC.Besides,as a key cell of immunity,whether CD4~+ T cell would involve in lung inflammation induced by particular matter is still unclear.This study aims to observe the effect of oBC on lung damage in mice and discuss how the functional MAP4K4 defect CD4~+ T cells(conditional knockout of MAP4K4) presents its role in this process.In our study,MAP4K4 deletion in CD4~+ T cells(MAP4K4cKO) could increase cell number of macrophages,lymphocytes and neutrophils in bronchoalveolar lavage fluid(BALF) exposed to oBC.MAP4K4 deletion in CD4~+ T cell also affected CD4~+ T cell differentiation in mediastinal lymph nodes after oBC stimulation.The number of CD4~+ IL17~+ T cell increased obviously.The levels of IL-6mRNA of lung in MAP4K4 cKO mice was higher than that in wild type mice after exposed to oBC,while the level of IL-6 in BALF had the same trend.Histological examination showed that MAP4K4 deletion in CD4~+ T cells affected lung inflammation induced by oBC.Results indicated that MAP4K4 cKO in CD4~+ T cells upgraded the level of inflammation in lung when exposed to oBC,which may be connected to the CD4~+ T cell differentiation and JNK,ERK and P38 pathways.
As the main composition of combustion,black carbon(BC) is becoming more and more noticeable at home and abroad.Ozone-oxidized black carbon(oBC)was produced through aging of ozone,one of the near-surface pollutants,to black carbon.And oBC was found to be more oxidation and cell toxicity when compared with BC.Besides,as a key cell of immunity,whether CD4~+ T cell would involve in lung inflammation induced by particular matter is still unclear.This study aims to observe the effect of oBC on lung damage in mice and discuss how the functional MAP4K4 defect CD4~+ T cells(conditional knockout of MAP4K4) presents its role in this process.In our study,MAP4K4 deletion in CD4~+ T cells(MAP4K4cKO) could increase cell number of macrophages,lymphocytes and neutrophils in bronchoalveolar lavage fluid(BALF) exposed to oBC.MAP4K4 deletion in CD4~+ T cell also affected CD4~+ T cell differentiation in mediastinal lymph nodes after oBC stimulation.The number of CD4~+ IL17~+ T cell increased obviously.The levels of IL-6mRNA of lung in MAP4K4 cKO mice was higher than that in wild type mice after exposed to oBC,while the level of IL-6 in BALF had the same trend.Histological examination showed that MAP4K4 deletion in CD4~+ T cells affected lung inflammation induced by oBC.Results indicated that MAP4K4 cKO in CD4~+ T cells upgraded the level of inflammation in lung when exposed to oBC,which may be connected to the CD4~+ T cell differentiation and JNK,ERK and P38 pathways.

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