Identification of regulatory DNA elements using genome-wide mapping of DNase I hypersensitive sites during tomato fruit development

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• 英文论文题名：Identification of regulatory DNA elements using genome-wide mapping of DNase I hypersensitive sites during tomato fruit development
• 论文作者：Zhengkun Qiu ; Ren Li ; Shuaibin Zhang ; Ketao Wang ; Meng Xu ; Jiayang Li ; Yongchen Du ; Hong Yu ; Xia Cui
• 英文论文作者：Zhengkun Qiu ; Ren Li ; Shuaibin Zhang ; Ketao Wang ; Meng Xu ; Jiayang Li ; Yongchen Du ; Hong Yu ; Xia Cui ; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture ; Sino-Dutch Joint Laboratory of Horticultural Genomics ; Institute of Vegetables and Flowers ; Chinese Academy of Agricultural Sciences ; State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing) ; Institute of Genetics and Developmental Biology ; Chinese Academy of Sciences
• 年：2016
• 作者机构：Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture,Sino-Dutch Joint Laboratory of Horticultural Genomics,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences;State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing),Institute of Genetics and Developmental Biology,Chinese Academy of Sciences;
• 英文论文关键词：DNase I hypersensitive sites ; cis-regulatory element ; gene expression ; fruit development ; tomato
• 会议召开时间：2016-10-09
• 会议录名称：2016年全国植物生物学大会摘要集
• 语种：英文
• 分类号：S641.2;Q943.2
• 学会代码：ZGZO
• 会议名称：2016年全国植物生物学大会
• 会议地点：中国湖北武汉
• 主办单位：中国遗传学会、中国细胞生物学学会、中国植物学会、中国植物生理与植物分子生物学学会、中国作物学会
• 学会名称：中国植物学会
• 页数：1
• 文件大小：904k
• 原文格式：D
• 会议级别：全国

摘要

Development and ripening of tomato fruit are precisely controlled by transcriptional regulation,which depends on the orchestrated accessibility of regulatory proteins to promoters and other cis-regulatory DNA elements. This accessibility and its effect on gene expression play a major role in defining the developmental process. To understand the regulatory mechanism and functional elements modulating morphological and anatomical changes during fruit development,we generated genomewide high-resolution maps of DNase I hypersensitive sites(DHSs) from the fruit tissues of the tomato cultivar ?Moneymaker? at 20 days post anthesis as well as break stage. By exploring variation of DHSs across fruit development stages,we pinpointed the most likely hypersensitive sites related to development-specific genes. By detecting binding motifs on DHSs of these development-specific genes or genes in the ascorbic acid biosynthetic pathway,we revealed the common regulatory elements contributing to coordinating gene transcription of plant ripening and specialized metabolic pathways. Our results contribute to a better understanding of the regulatory dynamics of genes involved in tomato fruit development and ripening.
Development and ripening of tomato fruit are precisely controlled by transcriptional regulation,which depends on the orchestrated accessibility of regulatory proteins to promoters and other cis-regulatory DNA elements. This accessibility and its effect on gene expression play a major role in defining the developmental process. To understand the regulatory mechanism and functional elements modulating morphological and anatomical changes during fruit development,we generated genomewide high-resolution maps of DNase I hypersensitive sites(DHSs) from the fruit tissues of the tomato cultivar ?Moneymaker? at 20 days post anthesis as well as break stage. By exploring variation of DHSs across fruit development stages,we pinpointed the most likely hypersensitive sites related to development-specific genes. By detecting binding motifs on DHSs of these development-specific genes or genes in the ascorbic acid biosynthetic pathway,we revealed the common regulatory elements contributing to coordinating gene transcription of plant ripening and specialized metabolic pathways. Our results contribute to a better understanding of the regulatory dynamics of genes involved in tomato fruit development and ripening.

引文