阳离子脂质体与基因复合物的结合动力学
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- 英文论文题名：The binding kinetics of cationic liposome and gene complex
- 论文作者：田甜 ; 赵轶男 ; 陈会英 ; 崔韶晖 ; 宋晨曦 ; 张树彪
- 英文论文作者：Tian Tian ; Yinan Zhao ; Huiying Chen ; Shaohui Cui ; Chenxi Song ; Shubiao Zhang ; Key Laboratory of Biotechnology and Bioresources Utilization-The State Ethnic Affairs Commission-Ministry of Education ; Dalian Minzu University
- 年：2016
- 作者机构：大连民族大学生物技术与资源利用国家民委-教育部重点实验室;
- 论文关键词：阳离子脂质体 ; 非病毒基因载体 ; 动力学 ; 荧光共振能量转移
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第二十八分会：化学生物学
- 语种：中文
- 分类号：R450
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第二十八分会 ; 化学生物学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：191k
- 原文格式：D
- 会议级别：全国
摘要
基因治疗作为一种全新的医疗手段,其有效性已得到临床试验的验证。基因递送载体技术是基因治疗的核心技术之一。阳离子脂质体作为一种重要的非病毒基因载体,在基因治疗领域具有广泛的应用前景。带正电荷的阳离子脂质体通过静电吸引作用与带负电荷的DNA结合,形成脂质体/DNA复合物。阳离子脂质体有效地与DNA结合是实现基因高效转运的必要条件,但另一方面,脂质体与DNA结合过于紧密,又不利于在细胞内载体对DNA的释放。本实验利用琼脂糖凝胶电泳技术检测脂质体与DNA的结合能力,通过荧光共振能量转移技术,即以上转换纳米晶与脂质体结合作为能量供体,以染料标记的探针DNA作为能量受体,建立荧光共振能量转移体系,利用荧光光谱测量体系的发光强度,研究脂质体与DNA的结合动力学,揭示阳离子脂质体和DNA结合的紧密程度与基因转运效率之间的内在联系。
As an important non-viral gene vector,cationic liposome had shown a potential application in gene transfer and disease treatment.It was necessary to combine cationic liposome with DNA efficiently for gene transfer.However,it was not conducive for intracellular release of DNA if cationic liposome was combined with DNA too tightly.In this study,agarose gel electrophoresis was used to evaluate the DNA binding ability of liposome.Fluorescence resonance energy transfer technology and fluorescence emission intensity spectrum were used to inspect the binding kinetics of cationic liposome and gene complexes.To establishmented of fluorescence resonance energy transfer system,upconversion nanoparticles combining with cationic liposome were acted as energy donor,and the labeled DNA was energy receptor.The luminescence intensity of fluorescence spectrum measurement system was used to study the binding kinetics of cationic liposome and gene complex.
As an important non-viral gene vector,cationic liposome had shown a potential application in gene transfer and disease treatment.It was necessary to combine cationic liposome with DNA efficiently for gene transfer.However,it was not conducive for intracellular release of DNA if cationic liposome was combined with DNA too tightly.In this study,agarose gel electrophoresis was used to evaluate the DNA binding ability of liposome.Fluorescence resonance energy transfer technology and fluorescence emission intensity spectrum were used to inspect the binding kinetics of cationic liposome and gene complexes.To establishmented of fluorescence resonance energy transfer system,upconversion nanoparticles combining with cationic liposome were acted as energy donor,and the labeled DNA was energy receptor.The luminescence intensity of fluorescence spectrum measurement system was used to study the binding kinetics of cationic liposome and gene complex.
引文
[1]王汝涛,陈涛,王昭,张阳.药物分析杂志,2009,11:1785
[2]Gopal,V.;Xavier,J.;Kamal,M Z.;et al.Bioconjugate chemistry,2011,22(11):2244.
[3]周继寒,牛林,梁德海.高分子通报,2014,08:34
[2]Gopal,V.;Xavier,J.;Kamal,M Z.;et al.Bioconjugate chemistry,2011,22(11):2244.
[3]周继寒,牛林,梁德海.高分子通报,2014,08:34
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