野生百合花器官愈伤组织诱导及生根研究
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- 英文论文题名：Induction and Rooting of the Callus from Floral Tissues of Wild Lilium Species
- 论文作者：孙道阳 ; 牛立新 ; 张延龙 ; 张新果 ; 李绍华
- 英文论文作者：SUN Dao-yang ; NIU Li-xin ; ZHANG Yan-long ; ZHANG Xin-guo ; LI Shao-hua ; College of Landscape Architecture and Arts ; Northwest A&F University
- 年：2016
- 作者机构：西北农林科技大学风景园林艺术学院;
- 论文关键词：野生百合 ; 花器官 ; 愈伤组织 ; 生根
- 英文论文关键词：Wild Lilium species ; Floral tissues ; Callus ; Rooting
- 会议召开时间：2016-07-19
- 会议录名称：中国观赏园艺研究进展2016
- 语种：中文
- 分类号：S682.29
- 学会代码：EGYP
- 会议名称：2016年中国观赏园艺学术研讨会
- 会议地点：中国湖南长沙
- 主办单位：中国园艺学会观赏园艺专业委员会、国家花卉工程技术研究中心
- 学会名称：中国园艺学会
- 页数：6
- 文件大小：535k
- 原文格式：O
- 会议级别：全国
摘要
以野生岷江百合、淡黄花百合、卷丹、宜昌百合、川百合、野百合以及东方百合杂种系‘西伯利亚'和‘索邦'的花丝和子房为外植体,以毒莠定(Picloram)为愈伤组织诱导剂,以吲哚丁酸(IBA)为生根剂,分别研究了不同浓度的Picloram和IBA对愈伤组织诱导和生根的影响。结果表明:花丝和子房为外植体都可诱导出黄色疏松的胚性愈伤组织,是愈伤组织诱导的良好材料;两个栽培品种比6个野生百合种更容易诱导出愈伤组织和生根;野生百合中,最高的平均诱导率发生在岷江百合花丝,培养基为MS+1.0mg/L Picloram,诱导率为79.3%±8.5%,子房的最高平均诱导率发生在野百合,培养基为MS+2.0mg/L Picloram,诱导率为78.0%±8.3%;最高平均生根率发生在野百合愈伤组织,培养基为MS+0.1mg/L IBA,生根率为79.5%±4.8%。
The filament and ovary of wild Lilium species,including L.regale,L.sulphureum,L.lancifolium,L.leucanthum,L.davidii and L.brownii,and Oriental Lily Hybrids‘Siberia' and‘Sorbonne' were used as explants.Picloram and IBA were used for induction and rooting of callus,respectively.The effect of different concentrations of Picloram and IBA on callus induction and rooting were studied.The results indicated that the filament and ovary were excellent materials to successfully induce yellow and loose embryogenic callus.Two cultivars were easier for callus induction and rooting than six wild Lilium species.Of the six wild Lilium species,the maximum average induction rate(79.3%±8.5%) occurred in L.regale filament and the used medium was MS+1.0mg/L Picloram.The maximum average induction rate(78.0%±8.3%)of ovary occurred in L.brownii and the used medium was MS+2.0mg/L Picloram.The highest average rooting rate(79.5%±4.8%) was found in callus of L.brownii and MS+0.1mg/L IBA was used as medium.
The filament and ovary of wild Lilium species,including L.regale,L.sulphureum,L.lancifolium,L.leucanthum,L.davidii and L.brownii,and Oriental Lily Hybrids‘Siberia' and‘Sorbonne' were used as explants.Picloram and IBA were used for induction and rooting of callus,respectively.The effect of different concentrations of Picloram and IBA on callus induction and rooting were studied.The results indicated that the filament and ovary were excellent materials to successfully induce yellow and loose embryogenic callus.Two cultivars were easier for callus induction and rooting than six wild Lilium species.Of the six wild Lilium species,the maximum average induction rate(79.3%±8.5%) occurred in L.regale filament and the used medium was MS+1.0mg/L Picloram.The maximum average induction rate(78.0%±8.3%)of ovary occurred in L.brownii and the used medium was MS+2.0mg/L Picloram.The highest average rooting rate(79.5%±4.8%) was found in callus of L.brownii and MS+0.1mg/L IBA was used as medium.
引文
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8.Qi Y,Du L,Quan Y,Tian F,Liu Y,Wang Y.2014.Agrobacterium-mediated transformation of embryogenic cell suspension cultures and plant regeneration in Lilium tenuifolium Oriental x Trumpet'Robina'[J].Acta Physiologiae Plantarum,36(8):2047-2057.
9.Rao J,Liu D,Zhang N,He H,Ge F,Chen C.2013.Identification of genes differentially expressed in a resistant reaction to Fusarium oxysporum in Lilium regale by SSH[J].Ieri Procedia,5(5):95-101.
10.Sharma A,Mahinghara B K,Singh A K,Kulshrestha S,Raikhy G,Singh L,Hallan V,Verma N,Raja R,Zaidi A A.2005.Identification,detection and frequency of lily viruses in Northern India[J].Scientia Horticulturae,106(2):213-227.
11.Tang YP,UuXQ,Gituru RW,Chen L Q.2010.Callus induction and plant regeneration from in vitro cultured leaves,petioles and scales of Lilium leucanthum(baker)baker[J].Biotechnology&Biotechnological Equipment,24(4):2071-2076.
12.王冬梅,黄学林,黄上志.1996.细胞分裂素类物质在植物组织培养中的作用机制[J].植物生理学报,32(5):373-377.
13.杨柏云,杨慧琴,蔡奇英,等.2005.龙牙百合体细胞胚的诱导及植株再生[J].南昌大学学报:理科版,29(6):536-539.
14.Yuan LL,LiuQ,Liu Q L,GrassottiA,Burchi G.2011.Conservation,evaluation and enhancement of wild lily germplasm in China[J].Acta Horticulturae,900(900):53-57.
15.袁雪,钟雄辉,李晓昕,等.2012.铁炮百合的胚性愈伤组织诱导和植株再生[J].核农学报,26(3):454-460.
16.张艺萍,吴丽芳,吴学尉,等.2008.东方百合胚性愈伤组织诱导和植株再生研究[J].江西农业学报,20(12):33-36.
2.Chandler S F,Brugliera F.2011.Genetic modification in floriculture[J].Biotechnology Letters,33(33):207-214.
3.高洁,王元忠,黄衡宇.2016.绿花百合胚性愈伤组织诱导与植株再生研究[J].植物研究,5(1):52-57.
4.Hoshi Y,Kondo M S,Adachi Y,Nakano M,Kobayashi H.2004.Production of transgenic lily plants by Agrobacteriummediated transformation[J].Plant Cell Reports,22(6):359-364.
5.胡凤荣,席梦利,刘光欣,等.2007.东方百合的器官发生与体胚发生研究[J].南京林业大学学报:自然科学版,31(2):5-8.
6.李莺,李星,李生玲,等.2013.‘黄天霸'百合花器官愈伤组织诱导及植株再生[J].热带作物学报,34(8):1507-1512.
7.农艳丰,杨美纯,宁云芬,等.2016.东方百合“甜梦”花器官组织培养再生植株研究[J].农业研究与应用,1:1-15.
8.Qi Y,Du L,Quan Y,Tian F,Liu Y,Wang Y.2014.Agrobacterium-mediated transformation of embryogenic cell suspension cultures and plant regeneration in Lilium tenuifolium Oriental x Trumpet'Robina'[J].Acta Physiologiae Plantarum,36(8):2047-2057.
9.Rao J,Liu D,Zhang N,He H,Ge F,Chen C.2013.Identification of genes differentially expressed in a resistant reaction to Fusarium oxysporum in Lilium regale by SSH[J].Ieri Procedia,5(5):95-101.
10.Sharma A,Mahinghara B K,Singh A K,Kulshrestha S,Raikhy G,Singh L,Hallan V,Verma N,Raja R,Zaidi A A.2005.Identification,detection and frequency of lily viruses in Northern India[J].Scientia Horticulturae,106(2):213-227.
11.Tang YP,UuXQ,Gituru RW,Chen L Q.2010.Callus induction and plant regeneration from in vitro cultured leaves,petioles and scales of Lilium leucanthum(baker)baker[J].Biotechnology&Biotechnological Equipment,24(4):2071-2076.
12.王冬梅,黄学林,黄上志.1996.细胞分裂素类物质在植物组织培养中的作用机制[J].植物生理学报,32(5):373-377.
13.杨柏云,杨慧琴,蔡奇英,等.2005.龙牙百合体细胞胚的诱导及植株再生[J].南昌大学学报:理科版,29(6):536-539.
14.Yuan LL,LiuQ,Liu Q L,GrassottiA,Burchi G.2011.Conservation,evaluation and enhancement of wild lily germplasm in China[J].Acta Horticulturae,900(900):53-57.
15.袁雪,钟雄辉,李晓昕,等.2012.铁炮百合的胚性愈伤组织诱导和植株再生[J].核农学报,26(3):454-460.
16.张艺萍,吴丽芳,吴学尉,等.2008.东方百合胚性愈伤组织诱导和植株再生研究[J].江西农业学报,20(12):33-36.
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